CN103190576A - Cordyceps militaris soybean and preparation method thereof - Google Patents
Cordyceps militaris soybean and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a cordyceps militaris soybean. A preparation method comprises the steps of soaking soybeans with water; carrying out sterilization on the soybeans after soaking; after cooling, feeding seed liquid of cordyceps bacteria for fermental cultivation; and then after fermentation, air-drying to obtain the cordyceps militaris soybean. When biological transformation is carried out on cordyceps in the soybeans, the seed coats of the soybeans have proper inhibition effect on the biological transformation. Therefore, after peeling treatment is carried out on the soaked soybeans, the cordycepin content after fermentation can be improved. Moreover, the inoculation area is increased and uniform, and the biological transformation amount of cordyceps cordycepin can be improved. Therefore, through cellulose treatment, the peeling rate of the soaked soybeans is improved; and since the soybeans is subjected to peeling, the transformation amount of cordycepin in the cordyceps militaris soybeans is improved.
Description
Technical field
The invention belongs to technical field of food preparation, be specifically related to a kind of Cordyceps militaris soya bean and preparation method thereof.
Background technology
Cordyceps militaris (Cordyceps militaris) is Ascomycotina, gang pyrenomycetes, Hypocreales, Clavicipitaceae, kind of Cordyceps, with Cordyceps sinensis (Cordyceps sinensis) for belonging to the xenogenesis fungi together, be commonly referred to northern Chinese caterpillar fungus.Owing to have identical medicinal and nourishing function with Cordyceps sinensis, and be easier to carry out artificial cultivation than Cordyceps sinensis, therefore Cordyceps militaris is chosen as the best substitute of Cordyceps sinensis and carries out a large amount of artificial cultures, and comes into the market as the approval that new resource food and a kind new medicine have obtained the Ministry of Public Health and State Food and Drug Administration.
The active component of Cordyceps militaris comprises cordycepin, adenosine, Cordyceps sinensis polysaccharide and cordycepic acid etc., wherein to the research of cordycepin, becomes focus both domestic and external in recent years.Cordycepin (Cordycepin) claim cordycepin again, namely 3 '-desoxyadenossine (3 '-Deoxyadenosine), be a kind of nucleoside antibiotic, have multiple physiologically active, as antibacterial, antitumor, inhibition virus function, and strengthen effects such as immunologic function and control cardiovascular and cerebrovascular disease.
The special medical health care of cordycepin has caused domestic and international expert's great attention, has much to put on market based on health products, health food, cosmetics, the medicine of cordycepin.It is reported, cordycepin is entered clinic trial as anticancer new antiviral drug in the U.S..Also will enter the first phase clinic trial by the synthetic leukemic new drug for the treatment of of cordycepin in China.
The pure fructification of the artificial Cordyceps militaris of cultivating, be used for human body as food, be completely after deliberation, can be widely used in health food, health diet and other nourishing based food, for example make cordycepin beverage, health care wine, nutritious health-care vinegar etc., but mostly be to be raw material with fructification or solid medium leaching liquor, the technology relative complex, cost is higher.Along with the progress of foods processing technique and people to the deepening continuously of Chinese caterpillar fungus research, the Chinese caterpillar fungus functional food should develop to the direction of diversification, processes meticulousr, fill a prescription more science, clearer and more definite, the more remarkable effect of function.And the approach by bio-transformation generates the cordycepin that does not have in the original food, and it is processed into food, meets the 21 century people to the requirement of food, and development prospect is wide.
Patent CN101171968A discloses by cereal solid-state fermentation Chinese caterpillar fungus and ganoderma lucidum product and manufacture method thereof, be to utilize different cereal such as heart of a lotus seed benevolence, rice, wheat, oat or feed as the tunning of Cordyceps sinensis or glossy ganoderma solid state fermentation, with the dry pulverize of this tunning, can add flour and flavor enhancement more in addition, namely can be made into health-care components such as being rich in dietary fiber, polysaccharide, and the Cordyceps sinensis or glossy ganoderma lotus-seed-heart powder, ground rice, wheat flour or the oatmeal that make things convenient for the consumer to brew to drink.
At present, with Chinese caterpillar fungus soya bean is carried out bio-transformation, namely produce the research of cordycepin in the solid fermentation of soya bean, at home and abroad not seeing has report.In China built a well-off society in an all-round way, if by the bio-transformation of soya bean and the functional components such as approach picked-up cordycepin of serial converted products thereof, to improving human health, significant, prospect was very wide.
Summary of the invention
The purpose of this invention is to provide a kind of Cordyceps militaris soya bean and preparation method thereof, thereby remedy the deficiencies in the prior art.
Cordyceps militaris soya bean of the present invention be with soya bean as solid medium, the inoculation cordyceps is cultivated preparation.
Cordyceps militaris soya bean of the present invention, its a kind of preparation method is as follows: the soya bean water is soaked, and soaked soybean is sterilized, and cooling back is inserted the seed liquor of cordyceps and is carried out fermented and cultured, air-dryly after the fermentation ends again obtains the Cordyceps militaris soya bean.
The seed liquor of cordyceps of the present invention, the seed culture medium of its preparation usefulness is composed as follows: potato 18～22%, glucose 1～3%, peptone 0.1～0.5%, potassium dihydrogen phosphate 0.03～0.1%, magnesium sulfate 0.05～0.5%, surplus is water.
Above-mentioned seed liquor also is added with Chinese true eupolyphoge sinensis peptide mixer, and the concentration of interpolation is 0.005%.
The true eupolyphoge sinensis peptide mixer of described China is after the true eupolyphoge sinensis powder of China is added distilled water, to carry out enzymolysis with papain, the preparation of enzymolysis liquid filtration drying.
Above-mentioned fermented and cultured is after 22 ℃ of following darkrooms are cultivated 5～8 days, then cultivates in bright chamber and finishes fermentation in 3～6 days.
In order to obtain better ferment effect, the soya bean of soaking is handled with enzyme before sterilization;
Describedly handling with enzyme, is the soya bean of soaking to be added the citric acid-sodium hydrogen phosphate buffer solution (pH is 4.5) that is added with cellulase carry out enzymolysis;
Described cellulose enzyme concentration is 8000～12000U/g soya bean, carries out enzyme under 50 ℃ and handles 1～3 hour.
The present invention also provides a kind of Cordyceps militaris culture mix, is Cordyceps militaris soya bean and Cordyceps militaris peanut mixt.
When Cordyceps militaris carried out bio-transformation in soya bean, the kind skin of soya bean had inhibitory action to it.Therefore, remove the peel processing to soaking soya bean, can improve the cordycepin content after its fermentation.So handle by cellulase, reach the purpose of soya bean peeling, thereby improve the cordycepin inversion quantity in the Cordyceps militaris soya bean.
The specific embodiment
Cordyceps of the present invention (Cordyceps militaris) can be selected the bacterial classification of the cordyceps of using in arbitrary field of food for use.And in order to make the vigor of cordyceps in culture medium higher, the present invention has carried out long term studies to the component of culture medium, and a kind of culture medium that can obviously strengthen the cordyceps vigor is provided.
Below in conjunction with embodiment Cordyceps militaris soya bean of the present invention and preparation method thereof is described in detail.
Embodiment 1
1) soybean soaking
Take by weighing the soya bean of 250g, added the water of 500g, at room temperature soak 12h, make the control of soaked soybean water content in 100～120% scopes.
2) sterilization
After soaked soybean drained, divide to install in the triangular flask, build stopper, under 121 ℃ of temperature, sterilized 30 minutes, wait after the cooling to inoculate.
3) the Cordyceps militaris spawn inclined-plane is cultivated
The Cordyceps militaris spawn of keeping is inoculated at slant medium, under 23 ℃ temperature, cultivated 7～9 days, consisting of of its slant medium: potato 18～22%, glucose 1～3%, peptone 0.1～1.0%, potassium dihydrogen phosphate 0.1～0.5%, magnesium sulfate 0.1～0.3%, Cobastab
10.008～
0.02%, agar 1～3%.Wherein concrete a kind of prescription is as follows: potato 20%, glucose 2%, peptone 0.5%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.25%, Cobastab
10.01%, agar 2%.The method of preparation is with the 20g potato cutting, adds 100mL water, boiled 20 minutes, boil with cooling procedure in constantly moisturizing, filter after finally transferring to 100mL.With 2g glucose, 0.5g peptone, 0.3g potassium dihydrogen phosphate, 0.25g magnesium sulfate, 0.01g Cobastab
1, 2g agar is dissolved in 100mL potato filtrate.
4) cultivation of Cordyceps militaris liquid seeds liquid
Get area and reach 10～30 ㎜
2The inclined-plane Cordyceps militaris spawn, receive in the seed liquor culture medium, under 20～25 ℃ temperature, cultivate and obtained seed liquor in 2～6 days.The mass percent of its liquid seed culture medium consists of: potato 18～22%, glucose 1～3%, peptone 0.1～0.5%, potassium dihydrogen phosphate 0.03～0.1%, magnesium sulfate 0.05～0.5%.
Wherein concrete a kind of prescription is as follows: potato 20%, glucose 2%, peptone 0.2%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.1%.The method of preparation is with the 20g potato cutting, adds 100mL water, boiled 20 minutes, boil with cooling procedure in constantly moisturizing, filter after finally transferring to 100mL.With 2g glucose, 0.2g peptone, 0.05g potassium dihydrogen phosphate, 0.1g magnesium sulfate, be dissolved in 100mL potato filtrate.
In order better to improve the fermentative activity of the cordyceps of cultivating, in the seed liquor culture medium, add Chinese true eupolyphoge sinensis peptide mixer, add quality concentration of volume percent 0.005%, namely add the Chinese true eupolyphoge sinensis peptide mixer of 0.005g in the 100ml culture medium.Wherein a kind of preparation method of Chinese true eupolyphoge sinensis peptide mixer is as follows:
Get the Chinese true eupolyphoge sinensis powder of 20g after freeze drying, add 400ml distilled water, regulate pH value to 8.2 with 10%NaOH, press the 300U/g raw material again and add papain, at 37 ℃ of following enzymolysis 5h.Enzymolysis liquid under 80 ℃, behind the enzyme 15min that goes out, with the centrifugal 15min of the rotating speed of 10000r/min, is got supernatant, obtain Chinese true eupolyphoge sinensis enzymolysis liquid.
For obtaining the low-molecular-weight peptide mixer, the supernatant of enzymolysis liquid is filtered with 2500Da molecular weight milipore filter, remove macro-molecular protein, collect filtered solution, filter with 250Da molecular weight milipore filter again, remove the small molecule salt class, collect trapped fluid and concentrate, freeze drying obtains molecular weight less than the Chinese true eupolyphoge sinensis mixtures of polypeptides of 2500Da.
5) expansion of Cordyceps militaris liquid seeds liquid is cultivated
With above-mentioned Cordyceps militaris liquid seeds liquid, receive in the expansion liquid seeds liquid culture medium by 8～15% volume ratio, under 20～25 ℃ temperature, carry out Cordyceps militaris and enlarge cultivation 2～6 days.
6) solid culture of Cordyceps militaris soya bean
Cultured Cordyceps militaris liquid is enlarged seed liquor, receive on the good soya bean of sterilization back cooling by 10～25%(v/w) inoculum concentration, after 22 ℃ of following darkrooms are cultivated 5～8 days, then cultivated 3～6 days in bright chamber; The brightness of described bright chamber is exactly 16 watts of the incandescent lamp power that arrange in the IS-RDU3 model isothermal vibration device.In the incubation of bright chamber, suitably stir.
7) drying
Cordyceps militaris soya bean after the fermentation ends, be yellow～orange-yellow, dry in the shade with natural wind, obtain Cordyceps militaris soya bean finished product.
8) cordycepin content assaying method
Adopt high performance liquid chromatography.
The extraction of cordycepin: after with the Cordyceps militaris soya bean that crushes, sieve (60 order), the solid-liquid ratio of pressing 1:20 adds 20% ethanol, extracts 30～60 minutes in ultrasonic device (40KHz, 40 ℃).After cordycepin extract to finish, under the 10000rpm rotating speed centrifugal 10 minutes.To utilize supernatant behind the 0.22um membrane filtration as the testing sample of measuring cordycepin.
Liquid-phase condition is that pillar uses KnauerC
18Column temperature, room temperature; Detect wavelength, 260nm; Mobile phase, acetonitrile: water=10:90; Flow velocity: 0.4mL/min.
Cordycepin content in the Cordyceps militaris soya bean of present embodiment preparation is at 10～13g/kg, compares with the cordycepin content in the silkworm chrysalis Cordyceps sinensis with the rice Cordyceps militaris and is increased to 2～5 times and 15～20 times respectively.
And by adding Chinese true eupolyphoge sinensis peptide mixer, make the cordycepin in the soya bean of subsequent preparation obtain increasing, and testing result shows, adds Chinese true eupolyphoge sinensis peptide mixer than under the situation of not adding, the content of the cordycepin in the soya bean has 25～40% lifting.
The kind of nitrogenous source, concentration and catabolite thereof all can the appreciable impact cometabolisms in the culture medium, and contain nitrogen-atoms in the molecular structure of many secondary metabolites, therefore nitrogenous source is except as the material that constitutes somatic cells, also can be directly as the precursor of secondary metabolite.Inorganic nitrogen-sourced because its composition is single, significant adverse is synthetic in cell growth and metabolite, during the employing compound nitrogen source, because of complexity and the changeability of its composition, the anabolism of cordycepin is had great importance.
Embodiment 2
1) soybean soaking
Take by weighing the soya bean of 250g, add water in the ratio of 1:2～1:5, at room temperature soak 10～16h, make the control of soya bean water content 100～120%.
2) the soya bean cellulase is handled
With soaked soya bean, be encased in after draining in the triangular flask and add citric acid-sodium hydrogen phosphate buffer solution by 1:3～5 solid-liquid ratios, carrying out cellulase under 50 ℃ handled 1～3 hour, enzyme concentration is 8000～12000U/g soya bean, drain after 10～20 minutes with flowing water flushing, drying in the shade dry 5～8 hours of place.Cellulase derives from green wooden enzyme, and vigor is 11000U/mg.
3) sterilization
10～15 orders peeling soya bean particle divides to install in the triangular flask, builds stopper, under 121 ℃ of temperature, sterilizes 30 minutes, waits after the cooling to inoculate.
4) the Cordyceps militaris spawn inclined-plane is cultivated
The Cordyceps militaris spawn of keeping is inoculated at slant medium, under 23 ℃ temperature, cultivated 7～9 days, consisting of of its slant medium: potato 18～22%, glucose 1～3%, peptone 0.1～1.0%, potassium dihydrogen phosphate 0.1～0.5%, magnesium sulfate 0.1～0.3%, Cobastab
10.008～
0.02%, agar 1～3%.Wherein concrete a kind of prescription is as follows: potato 20%, glucose 2%, peptone 0.5%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.25%, Cobastab
10.01%, agar 2%.The method of preparation is with the 20g potato cutting, adds 100mL water, boiled 20 minutes, boil with cooling procedure in constantly moisturizing, filter after finally transferring to 100mL.With 2g glucose, 0.5g peptone, 0.3g potassium dihydrogen phosphate, 0.25g magnesium sulfate, 0.01g Cobastab
1, 2g agar is dissolved in 100mL potato filtered fluid.
5) cultivation of Cordyceps militaris liquid seeds liquid
Get area and reach 10～30 ㎜
2The inclined-plane Cordyceps militaris spawn, receive in the seed liquor culture medium, under 20～25 ℃ temperature, cultivate and obtained seed liquor in 2～6 days.The mass percent of its liquid seed culture medium consists of: potato 18～22%, glucose 1～3%, peptone 0.1～0.5%, potassium dihydrogen phosphate 0.03～0.1%, magnesium sulfate 0.05～0.5%.
Wherein concrete a kind of prescription is as follows: potato 20%, glucose 2%, peptone 0.2%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.1%.The method of preparation is with the 20g potato cutting, adds 100mL water, boiled 20 minutes, boil with cooling procedure in constantly moisturizing, filter after finally transferring to 100mL.With 2g glucose, 0.2g peptone, 0.05g potassium dihydrogen phosphate, 0.1g magnesium sulfate, be dissolved in 100mL potato filtered fluid.
6) expansion of Cordyceps militaris liquid seeds liquid is cultivated
With above-mentioned Cordyceps militaris liquid seeds liquid, receive in the expansion liquid seeds liquid culture medium by 8～15% volume ratio, under 20～25 ℃ temperature, carry out Cordyceps militaris and enlarge cultivation 2～6 days.
In order better to improve the fermentative activity of the cordyceps of cultivating, in the seed liquor culture medium, add Chinese true eupolyphoge sinensis peptide mixer, add mass percent concentration 0.005%, namely add the Chinese true eupolyphoge sinensis peptide mixer of 0.005g in the 100ml culture medium.Wherein a kind of preparation method of Chinese true eupolyphoge sinensis peptide mixer is as follows:
Get the Chinese true eupolyphoge sinensis powder of 20g after freeze drying, add 400ml distilled water, regulate pH value to 8.2 with 10%NaOH, press the 300U/g raw material again and add papain, at 37 ℃ of following enzymolysis 5h.Enzymolysis liquid under 80 ℃, behind the enzyme 15min that goes out, with the centrifugal 15min of the rotating speed of 10000r/min, is got supernatant, obtain Chinese true eupolyphoge sinensis enzymolysis liquid.
For obtaining the low-molecular-weight peptide mixer, the supernatant of enzymolysis liquid is filtered with 2500Da molecular weight milipore filter, remove macro-molecular protein, collect filtered solution, filter with 250Da molecular weight milipore filter again, remove the small molecule salt class, collect trapped fluid and concentrate, freeze drying obtains molecular weight less than the Chinese true eupolyphoge sinensis mixtures of polypeptides of 2500Da.
7) solid culture of Cordyceps militaris soya bean
Cultured Cordyceps militaris liquid is enlarged seed liquor, receive on the good soya bean of sterilization back cooling by 10～25%(v/w) inoculum concentration, after 22 ℃ of following darkrooms are cultivated 5～8 days, then cultivated 3～6 days in bright chamber; The brightness of described bright chamber is exactly 16 watts of the incandescent lamp power that arrange in the IS-RDU3 model isothermal vibration device.In the incubation of bright chamber, suitably stir.
8) drying
Cordyceps militaris soya bean after the fermentation ends, be yellow～orange-yellow, dry in the shade with natural wind, obtain Cordyceps militaris soya bean finished product.
Cordycepin content in the Cordyceps militaris soya bean of utilization peeling soya bean preparation is not compared with removing the peel soya bean at 15～17g/kg, and cordycepin content promotes 30～50%.
Embodiment 3
1) soybean soaking
Take by weighing the soya bean of 250g, add water in the ratio of 1:2～1:5, at room temperature soak 10～16h, make the control of soya bean water content 100～120%.
2) the soya bean cellulase is handled
With soaked soya bean, be encased in after draining in the triangular flask and add citric acid-sodium hydrogen phosphate buffer solution by 1:3～5 solid-liquid ratios, carrying out cellulase under 50 ℃ handled 1～3 hour, enzyme concentration is 8000～12000U/g soya bean, drain after 10～20 minutes with flowing water flushing, drying in the shade dry 5～8 hours of place.
3) coupling of two kinds of composition solid mediums
After the ratio mixing of the peanut after the soya bean after cellulase is handled and the peeling in 1:0.1～1.0, the ratio in 2:0.5～2.5 adds the water mixing again, and is subject to sterilization.
4) sterilization
With two kinds of composition solid mediums that match by a certain percentage, build stopper, under 121 ℃ of temperature, sterilized 30 minutes, wait after the cooling to inoculate.
5) the Cordyceps militaris spawn inclined-plane is cultivated
The Cordyceps militaris spawn of keeping is inoculated at slant medium, under 23 ℃ temperature, cultivated 7～9 days, consisting of of its slant medium: potato 18～22%, glucose 1～3%, peptone 0.1～1.0%, potassium dihydrogen phosphate 0.1～0.5%, magnesium sulfate 0.1～0.3%, Cobastab
10.008～
0.02%, agar 1～3%.Wherein concrete a kind of prescription is as follows: potato 20%, glucose 2%, peptone 0.5%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.25%, Cobastab
10.01%, agar 2%.The method of preparation is with the 20g potato cutting, adds 100mL water, boiled 20 minutes, boil with cooling procedure in constantly moisturizing, filter after finally transferring to 100mL.With 2g glucose, 0.5g peptone, 0.3g potassium dihydrogen phosphate, 0.25g magnesium sulfate, 0.01g Cobastab
1, 2g agar is dissolved in 100mL potato filtered fluid.
6) cultivation of Cordyceps militaris liquid seeds liquid
Get area and reach 10～30 ㎜
2The inclined-plane Cordyceps militaris spawn, receive in the seed liquor culture medium, under 20～25 ℃ temperature, cultivate and obtained seed liquor in 2～6 days.The mass percent of its liquid seed culture medium consists of: potato 18～22%, glucose 1～3%, peptone 0.1～0.5%, potassium dihydrogen phosphate 0.03～0.1%, magnesium sulfate 0.05～0.5%.
Wherein concrete a kind of prescription is as follows: potato 20%, glucose 2%, peptone 0.2%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.1%.The method of preparation is with the 20g potato cutting, adds 100mL water, boiled 20 minutes, boil with cooling procedure in constantly moisturizing, filter after finally transferring to 100mL.With 2g glucose, 0.2g peptone, 0.05g potassium dihydrogen phosphate, 0.1g magnesium sulfate, be dissolved in 100mL potato filtered fluid.
7) expansion of Cordyceps militaris liquid seeds liquid is cultivated
With above-mentioned Cordyceps militaris liquid seeds liquid, receive in the expansion liquid seeds liquid culture medium by 8～15% volume ratio, under 20～25 ℃ temperature, carry out Cordyceps militaris and enlarge cultivation 2～6 days.
In order better to improve the fermentative activity of the cordyceps of cultivating, in the seed liquor culture medium, add Chinese true eupolyphoge sinensis peptide mixer, add mass percent concentration 0.005%.Wherein the preparation method of the Chinese true eupolyphoge sinensis peptide mixer of Tian Jiaing is as follows:
Get the degreasing China true eupolyphoge sinensis powder of 20g after freeze drying, press the 1:20 material-water ratio and add distilled water, regulate pH value to 8.2 with 10%NaOH, press the 300U/g raw material again, add papain, at 37 ℃ of following enzymolysis 5h.Enzymolysis liquid under 80 ℃, behind the enzyme 15min that goes out, with the centrifugal 15min of the rotating speed of 10000r/min, is got supernatant, obtain Chinese true eupolyphoge sinensis enzymolysis liquid.
For obtaining the low-molecular-weight peptide mixer, the centrifugal supernatant that obtains of enzymolysis liquid is filtered with 2500Da molecular weight milipore filter, remove macro-molecular protein, collect filtered solution, filter with 250Da molecular weight milipore filter again, remove the small molecule salt class, collect trapped fluid and concentrate, freeze drying obtains molecular weight less than the Chinese true eupolyphoge sinensis mixtures of polypeptides of 2500Da.
8) with the peanut be the Cordyceps militaris soya bean solid culture of auxiliary material
Cultured Cordyceps militaris liquid is enlarged seed liquor, receive on the good soya bean of sterilization back cooling by 10～25%(v/w) inoculum concentration, after 22 ℃ of following darkrooms are cultivated 5～8 days, then cultivated 3～6 days in bright chamber; The brightness of described bright chamber is exactly 16 watts of the incandescent lamp power that arrange in the IS-RDU3 model isothermal vibration device.In the incubation of bright chamber, suitably stir.
8, drying
Cordyceps militaris soya bean after the fermentation ends, be yellow～orange-yellow, dry in the shade with natural wind, obtain Cordyceps militaris soya bean finished product.
The Cordyceps militaris soya bean of the present invention's preparation improves immunity and antioxidant effect detects:
Mouse feeding respectively contains the 0(basal diet), the daily ration of 3%, 6%, 12% Cordyceps militaris soya bean (embodiment 2 preparation) and 12% common soya bean, with MDA content in mice plasma and the liver, CAT, GSH-Px, T-SOD vigor and inhibition hydroxy radical ability are index, study antioxidation activity (injected in mice D-galactolipin is set up aging model) in its body; Measure immunoglobulin G, M content and cell factor interleukin-4, gamma interferon content in blood plasma and the liver organization, the research immune function of mice.The result shows, add the Cordyceps militaris soya bean and can significantly improve CAT, GSH-Px in mice plasma and the liver, T-SOD (P＜0.05) vigor, the content that suppresses hydroxy radical ability and IgG, IgM, IL-4, IFN-γ (P＜0.05), mouse spleen and liver index be can significantly improve and MDA (P＜0.05) content, the effect that has delaying senility and improve body's immunity significantly reduced.
Claims (10)
1. Cordyceps militaris soya bean, described Cordyceps militaris soya bean be with soya bean as solid medium, inoculate cordyceps and cultivate preparation.
2. the preparation method of the described Cordyceps militaris soya bean of claim 1 is that the soya bean water is soaked, and soaked soybean is sterilized, and inserts the seed liquor of cordyceps and carries out fermented and cultured, more air-dry obtaining.
3. preparation method as claimed in claim 2, the seed liquor that it is characterized in that described cordyceps, the seed culture medium of its preparation usefulness is composed as follows: potato 18～22%, glucose 1～3%, peptone 0.1～0.5%, potassium dihydrogen phosphate 0.01～0.06%, magnesium sulfate 0.1～0.3%, surplus is water.
4. preparation method as claimed in claim 3 is characterized in that, described seed culture medium is added with Chinese true eupolyphoge sinensis peptide mixer, and the concentration of interpolation is 0.005%.
5. preparation method as claimed in claim 4 is characterized in that the true eupolyphoge sinensis peptide mixer of described China, be after the true eupolyphoge sinensis powder of China is added distilled water, to carry out enzymolysis with papain, the preparation of enzymolysis liquid filtration drying.
6. preparation method as claimed in claim 2 is characterized in that described fermented and cultured, is after 22 ℃ of following darkrooms are cultivated 5～8 days, then finishes in 3～6 days in the cultivation of bright chamber.
7. preparation method as claimed in claim 2 is characterized in that the soya bean of described immersion is handled with enzyme before sterilization.
8. preparation method as claimed in claim 7 is characterized in that describedly handling with enzyme, is the soya bean of soaking to be added the citric acid-sodium hydrogen phosphate buffer solution that is added with cellulase carry out enzymolysis.
9. preparation method as claimed in claim 8 is characterized in that described cellulose enzyme concentration is 8000～12,000 ten thousand U/g soya beans; Carrying out enzyme under 50 ℃ handled 1～3 hour.
10. Cordyceps militaris culture mix, described mixture is the mixture of the described Cordyceps militaris soya bean of claim 1 and Cordyceps militaris peanut.
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| CN104824728A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis sesames |
| CN104824574A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis broad bean |
| CN104824586A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis cassava |
| CN104824588A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis potatoes |
| CN105685255A (en) * | 2016-02-22 | 2016-06-22 | 遵义医学院 | Method for manufacturing cordyceps militaris tofu |
| CN108684824A (en) * | 2018-06-12 | 2018-10-23 | 徐州工程学院 | A kind of Cordyceps militaris fermentation soybean soya-bean milk and preparation method thereof |
| CN108841904A (en) * | 2018-07-20 | 2018-11-20 | 徐州工程学院 | A kind of Cordyceps militaris fermentation soybean peptide and preparation method thereof |
| CN108850176A (en) * | 2018-06-12 | 2018-11-23 | 徐州工程学院 | A kind of Cordyceps militaris spawn fermentation soybean soymilk and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1266645A (en) * | 2000-03-02 | 2000-09-20 | 王养军 | Process for preparing fungal flour, fungal bean powder nad fungal bean-milk powder as food or medicine |
| KR100796303B1 (en) * | 2007-06-26 | 2008-01-21 | 박동기 | Manufactruring method of the germiated soybean cordyceps militaris having a bird flu preventing function |
| CN101991045A (en) * | 2009-08-19 | 2011-03-30 | 朴美子 | Cordyceps militaris rice and solid fermentation preparation method thereof |
| CN102274271A (en) * | 2010-06-13 | 2011-12-14 | 深圳市每人康生物科技有限公司 | Soybean plantworms mycelium powder and preparation method thereof |
| CN102334678A (en) * | 2011-07-11 | 2012-02-01 | 范根生 | Method for producing cordyceps mycelium powder by utilizing sprouted soybeans |
-
2013
- 2013-04-27 CN CN201310150267.4A patent/CN103190576B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1266645A (en) * | 2000-03-02 | 2000-09-20 | 王养军 | Process for preparing fungal flour, fungal bean powder nad fungal bean-milk powder as food or medicine |
| KR100796303B1 (en) * | 2007-06-26 | 2008-01-21 | 박동기 | Manufactruring method of the germiated soybean cordyceps militaris having a bird flu preventing function |
| CN101991045A (en) * | 2009-08-19 | 2011-03-30 | 朴美子 | Cordyceps militaris rice and solid fermentation preparation method thereof |
| CN102274271A (en) * | 2010-06-13 | 2011-12-14 | 深圳市每人康生物科技有限公司 | Soybean plantworms mycelium powder and preparation method thereof |
| CN102334678A (en) * | 2011-07-11 | 2012-02-01 | 范根生 | Method for producing cordyceps mycelium powder by utilizing sprouted soybeans |
Non-Patent Citations (4)
| Title |
|---|
| 彭志妮等: "蛹虫草固体发酵大豆基质的成分及抗氧化活性变化研究", 《菌物学报》 * |
| 王懋存等: "中华真地鳖抗氧化肽的分离及其抗氧化活性的研究", 《中国食品学报》 * |
| 王菊凤等: "蛹虫草的光温反应及生长发育特性", 《山地农业生物学报》 * |
| 相沢孝亮等: "《酶应用手册》", 28 February 1989, 上海科学技术出版社 * |
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| CN104824728A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis sesames |
| CN104824574A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis broad bean |
| CN104824586A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis cassava |
| CN104824588A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis potatoes |
| CN105685255A (en) * | 2016-02-22 | 2016-06-22 | 遵义医学院 | Method for manufacturing cordyceps militaris tofu |
| CN108684824A (en) * | 2018-06-12 | 2018-10-23 | 徐州工程学院 | A kind of Cordyceps militaris fermentation soybean soya-bean milk and preparation method thereof |
| CN108850176A (en) * | 2018-06-12 | 2018-11-23 | 徐州工程学院 | A kind of Cordyceps militaris spawn fermentation soybean soymilk and preparation method thereof |
| CN108841904A (en) * | 2018-07-20 | 2018-11-20 | 徐州工程学院 | A kind of Cordyceps militaris fermentation soybean peptide and preparation method thereof |
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