CN108371282A - A kind of cicada fungus rice and preparation method thereof - Google Patents

A kind of cicada fungus rice and preparation method thereof Download PDF

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Publication number
CN108371282A
CN108371282A CN201610889790.2A CN201610889790A CN108371282A CN 108371282 A CN108371282 A CN 108371282A CN 201610889790 A CN201610889790 A CN 201610889790A CN 108371282 A CN108371282 A CN 108371282A
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culture
rice
preparation
medium
strain
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CN201610889790.2A
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CN108371282B (en
Inventor
吴凯强
范冬晓
陈冬冬
李成
彭国杰
徐煜
孙长胜
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Zhejiang Panya Health Food Co., Ltd.
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Zhejiang Faya Biological Pharmaceutical Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

The present invention relates to a kind of cicada fungus rice and preparation method thereof.This method is that the Cordyceps cicadae strain liquid Jing Guo Liquid Culture is inoculated into rice solid medium, by the solid fermentation of light culture, obtains a kind of colored cicada fungus rice.Cicada fungus rice prepared by the present invention is in aubergine, and nutritive value is high.

Description

A kind of cicada fungus rice and preparation method thereof
Technical field
The present invention relates to a kind of cicada fungus rice and preparation method thereof, belong to biotechnology.
Background technology
Cicada fungus is traditional rare Chinese medicine of medicine-food two-purpose, protein content 39.35%, polyoses content 4.44%, Fat content is 6.97%, and contains various trace elements.And with the continuous development of biotechnology, the research master of cicada fungus at present It concentrates on and cultivates quality strains, high yield is obtained using new type of rearing, it is less for the deep processing research of cicada fungus.It is how sharp It is problem to be solved by this invention with cicada fungus exploitation nutritive value height and edible safety, convenient product.
Invention content
The purpose of the present invention is to provide a kind of preparation method of cicada fungus rice, this method is by the cicada Jing Guo Liquid Culture Flower strain liquid is inoculated into rice solid medium, by the solid fermentation of light culture, obtains a kind of colored cicada fungus rice.This hair The cicada fungus rice of bright preparation is in aubergine, and nutritive value is high, rich in dietary fiber, ferro element, vitamin B6, vitamin C with And the amino acid such as aspartic acid, threonine, glycine, arginine, it is a kind of edible safety, conveniently, various people can be met and wanted The nutrient rice asked.
The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of preparation method of cicada fungus rice, this method comprises the following steps:
Step a, the culture of strain:Cordyceps cicadae strain is accessed in fluid nutrient medium and is cultivated, strain liquid is obtained;
Step b, the processing of rice:Rice plus water are steamed;
Step c, inoculation:The step a strain liquids cultivated are inoculated into the rice that step b processing obtains;
Step d, culture:It carries out the rice after step c inoculations to be protected from light culture;
Step e is dry.
Preferably, whole preparation process will ensure sterile, and all equipment are into sterilization treatment excessively, the method packet of the sterilizing Include 121 DEG C, 30min autoclave sterilizations and 30min superclean bench ultraviolet sterilizations.
Preferably, fluid nutrient medium described in step a is the fluid nutrient medium of this field routine, such as potato-sucrose-agar Culture medium (PSA), Potato-dextrose-agar medium (PDA), Potato-dextrose-water culture medium (PSB), yeast leaching Go out powder-compound amino acid-sucrose culture medium (SAAY), yeast extract powder-white granulated sugar-soy bean protein hydrolysate culture medium, wheat bran boils Any one in juice-white sand sugar culture-medium, fish meal protein peptone-yeast extract powder-dextrose culture-medium;It is further preferred that The fluid nutrient medium is fish meal protein peptone-yeast extract powder-dextrose culture-medium;It is furthermore preferred that the Liquid Culture basigamy Fang Wei:Fish meal protein peptone 10g/L, yeast extract powder 10g/L, glucose 40g/L.
Preferably, cultural method described in step a is shaking table culture, and shaking table culture condition is:22~25 DEG C of temperature, 150~ 180rmp/min is protected from light 60~72h of culture.
Preferably, it is about 15-18% to steam rice to water content described in step b;
Preferably, step c inoculum concentrations are rice (g) and strain liquid (ml) ratio is (4~6):1;
Preferably, the inoculation method described in step c is to pour into strain liquid in rice, and stirring makes strain liquid and rice fill Divide mixing.
Preferably, step d condition of culture is:20~25 DEG C of temperature, incubation time 2~7 days;It is further preferred that culture It to be sufficiently stirred once every 10~12h in the process.
Invention further provides the cicada fungus rice that the above method is prepared.
Beneficial effects of the present invention:1. cultural method is simple, raw material only needs Cordyceps cicadae strain and big in solid fermentation process Meter, it is not necessary that any nutrient solution is added in incubation;2. beautiful appearance, the solid culture stage of the present invention only carry out light culture without Optical culture is carried out, the cicada fungus rice of preparation remains the color of CSM treated body, is in aubergine, more compared to traditional white rice It is ornamental;3. rich in nutrition content, cicada fungus rice its dietary fiber prepared by the present invention, ferro element, vitamin B6, vitamin The amino acid contents such as C and aspartic acid, threonine, glycine, arginine are above general rice, especially arginine content It is higher by 116.67% than standard.
Specific implementation mode
The Cordyceps cicadae strain used in following embodiment is Paecilomyces cicadae bacterial strain disclosed in CN102851353A [Paecilomyces cicadae (Miq.) Samson], the bacterial strain are protected on November 18th, 2009 in Chinese microorganism strain It hides administration committee's common micro-organisms center (abbreviation CGMCC) and registers preservation, preserving number is CGMCC No.3453.Cicada fungus bacterial strain Not as limiting the scope of the invention, cicada fungus bacterial strain disclosed in commercially available or other prior arts can realize the present invention's Effect.
Embodiment 1
Step a, the culture of strain
The configuration of fluid nutrient medium:Weigh yeast extract powder 5g respectively, fish meal protein peptone 5g, glucose 20g are added water to 500mL is stirred well to after being completely dissolved, in packing to the conical flask of 4 250mL.By sterile water and culture medium newspaper After wrapping, 121 DEG C of sterilizing 30min in autoclave sterilization pot are placed in, after taking-up to be cooled, are placed in superclean bench ultraviolet sterilization 30min。
Inoculation:2 Cordyceps cicadae strain liquid are taken, after being separately added into sterile water, conidium is scraped with oese, shakes up, pour into In conical flask, 2 conical flasks of one of inclined plane inoculating.
Shaking table culture:Conical flask is placed in shaking table and is cultivated, wherein condition of culture is 22 DEG C, 150rmp/min, light culture 60h, after culture, refrigeration is spare.
Step b, the processing of rice
1600g rice is weighed, is eluriated;With electromagnetic oven by clear water it is boiled after, in steaming 3-5min on food steamer, until rice is aqueous Amount can be taken off 15~18%.In packing to the happy button box of autoclave sterilization, per box 400g rice.It is placed in superclean bench Ultraviolet sterilization 30min.
Step c, inoculation
Strain liquid 100mL is added in the strain liquid for taking step a to cultivate in each happy button box, after mixing well, and Rice is laid on happy button box.
Step d, culture
The rice that finishes of inoculation is placed in constant humidity cabinet and is cultivated, condition of culture is 22 DEG C, light culture 48h, in incubation Rice need to be stirred every 8-12h 1 time, and stirring rice need to be in superclean bench, should be in advance to superclean bench ultraviolet sterilization 30min。
Step e is dry
Cultured rice is placed under dry environment and is dried, rice need to be crumpled every 2-3h, drying finished big Rice packs after crossing 10 mesh screens, every bag of about 500g cicada fungus rice, is preserved after vacuum sealing.Cicada fungus rice appearance of the present invention is purplish red Color.
Composition detection is carried out to cicada fungus rice manufactured in the present embodiment, the results are shown in Table 1-2.
1 nutritional ingredient testing result of table
2 amino acid content testing result of table
By table 1~2 as it can be seen that its protein of cicada fungus rice manufactured in the present embodiment, dietary fiber, ferro element, vitamin B6, Vitamin C content is above commercially available rice, especially Vitamin C content and is higher by 29 times compared with standard, and iron content is higher by 1.67 Times;In addition, rice fat content manufactured in the present embodiment and saturated fatty acid content are substantially reduced;Cicada fungus manufactured in the present embodiment The a variety of amino acid contents of rice are above standard, especially arginine content and than standard are higher by 116.67% (arginine is in cell Key player is all play during division, wound recovery, discharge ammonia, immune function, secreting hormone, protein etc., as one The precursor of nitrogen oxide, arginine can assist vasodilator, powerful to hyperammonemia, hepatic dysfunction etc.).
Embodiment 2
Substantially the same manner as Example 1, difference lies in fluid nutrient medium is Potato-dextrose-agar medium.
Embodiment 3
Substantially the same manner as Example 1, difference lies in fluid nutrient medium is yeast extract powder-compound amino acid-sucrose culture Base.
Embodiment 4
Substantially the same manner as Example 1, difference lies in inoculum concentration is to be inoculated with 100ml strain liquids per 500g rice.
Embodiment 5
Substantially the same manner as Example 1, difference lies in inoculum concentration is to be inoculated with 100ml strain liquids per 600g rice.
Embodiment 6
Substantially the same manner as Example 1, difference lies in the light culture time is 72h.

Claims (10)

1. a kind of preparation method of cicada fungus rice, which is characterized in that described method includes following steps:
Step a, the culture of strain:Cordyceps cicadae strain is accessed in fluid nutrient medium and is cultivated, strain liquid is obtained;
Step b, the processing of rice:Rice plus water are steamed;
Step c, inoculation:The step a strain liquids cultivated are inoculated into the rice that step b processing obtains;
Step d, culture:It carries out the rice after step c inoculations to be protected from light culture;
Step e is dry.
2. preparation method as described in claim 1, which is characterized in that fluid nutrient medium described in step a is selected from potato-sugarcane Sugar-agar medium, Potato-dextrose-agar medium, Potato-dextrose-water culture medium, yeast extract powder-are compound Amino acid-sucrose culture medium, yeast extract powder-white granulated sugar-soy bean protein hydrolysate culture medium, wheat bran liquor-white sand sugar culture-medium, Any one in fish meal protein peptone-yeast extract powder-dextrose culture-medium.
3. preparation method as claimed in claim 2, which is characterized in that the fluid nutrient medium is fish meal protein peptone-yeast leaching Go out powder-dextrose culture-medium.
4. preparation method as claimed in claim 3, which is characterized in that the Liquid Culture based formulas is:Fish meal protein peptone 10g/L, yeast extract powder 10g/L, glucose 40g/L.
5. preparation method as described in claim 1, which is characterized in that cultural method described in step a is shaking table culture, shaking table training Foster condition is:22~25 DEG C of temperature, 150~180rmp/min are protected from light 60~72h of culture.
6. preparation method as described in claim 1, which is characterized in that it is 15- to steam rice to water content described in step b 18%.
7. preparation method as described in claim 1, which is characterized in that step c inoculum concentrations are the weighing body of rice and strain liquid Product is than being (4~6):1.
8. preparation method as described in claim 1, which is characterized in that step c inoculation methods are to pour into strain liquid in rice, And stirring makes strain liquid and rice mix well.
9. preparation method as described in claim 1, which is characterized in that step d condition of culture is:20~25 DEG C of temperature, culture 2~7 days time.
10. the cicada fungus rice being prepared such as claim 1-9 any one of them preparation methods.
CN201610889790.2A 2016-10-12 2016-10-12 Cordyceps sobolifera rice and preparation method thereof Active CN108371282B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109259198A (en) * 2018-10-16 2019-01-25 镇江叶脉康食品生物科技有限公司 The method for preparing blood sugar reducing food using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050002704A (en) * 2004-11-22 2005-01-10 문창현 Food compositions comprising Isaria sinclairii as an effective component
CN102132881A (en) * 2011-03-29 2011-07-27 胡海波 Method for preparing lucid ganoderma rice food
CN102160642A (en) * 2011-03-17 2011-08-24 李佃场 Method for preparing Cordyceps rice food
CN102172275A (en) * 2011-03-29 2011-09-07 胡海波 Method for producing agaricus blazei rice
CN102242154A (en) * 2010-05-14 2011-11-16 浙江泛亚生物医药股份有限公司 Liquid fermentation method for producing paecilomyces cicadae mycelia and application of culture product
CN102552335A (en) * 2011-02-22 2012-07-11 上海泛亚生命科技有限公司 Traditional Chinese medicine health care product, its preparation method and its application
CN102805335A (en) * 2012-07-20 2012-12-05 黄晓青 Method for preparing edible fungus health-care product
CN103392500A (en) * 2013-06-24 2013-11-20 浙江大学 Culture method for cordyceps sobolifera

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050002704A (en) * 2004-11-22 2005-01-10 문창현 Food compositions comprising Isaria sinclairii as an effective component
CN102242154A (en) * 2010-05-14 2011-11-16 浙江泛亚生物医药股份有限公司 Liquid fermentation method for producing paecilomyces cicadae mycelia and application of culture product
CN102552335A (en) * 2011-02-22 2012-07-11 上海泛亚生命科技有限公司 Traditional Chinese medicine health care product, its preparation method and its application
CN102160642A (en) * 2011-03-17 2011-08-24 李佃场 Method for preparing Cordyceps rice food
CN102132881A (en) * 2011-03-29 2011-07-27 胡海波 Method for preparing lucid ganoderma rice food
CN102172275A (en) * 2011-03-29 2011-09-07 胡海波 Method for producing agaricus blazei rice
CN102805335A (en) * 2012-07-20 2012-12-05 黄晓青 Method for preparing edible fungus health-care product
CN103392500A (en) * 2013-06-24 2013-11-20 浙江大学 Culture method for cordyceps sobolifera

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109259198A (en) * 2018-10-16 2019-01-25 镇江叶脉康食品生物科技有限公司 The method for preparing blood sugar reducing food using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder

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