CN108244355A - A kind of method for improving quality of mutton - Google Patents

A kind of method for improving quality of mutton Download PDF

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Publication number
CN108244355A
CN108244355A CN201711470350.4A CN201711470350A CN108244355A CN 108244355 A CN108244355 A CN 108244355A CN 201711470350 A CN201711470350 A CN 201711470350A CN 108244355 A CN108244355 A CN 108244355A
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strain
cordyceps
mutton
cordyceps sinensis
fermentation
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李晓祥
徐自奥
曹赞
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HEFEI MICRO BIOLOGICAL ENGINEERING Co Ltd
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HEFEI MICRO BIOLOGICAL ENGINEERING Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/02—Breeding vertebrates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/26—Compounds containing phosphorus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The present invention provides a kind of method for improving quality of mutton, this method includes feeding mutton sheep using Cordyceps strain fermentation object as feed addictive.Present invention discover that feeding mutton sheep using Cordyceps strain fermentation object, the content of protein in mutton can be significantly improved, reduces the pH value and drip loss of Boar taint substances, the content of cholesterol and fat and mutton in mutton, and then realize the raising of quality of mutton.

Description

A kind of method for improving quality of mutton
Technical field
The present invention relates to livestock and poultry cultivation technical fields, and in particular to a kind of method for improving quality of mutton.
Background technology
With the development of society, living standards of the people be continuously improved, diet idea also gradually changes, and people are to meat The requirement of class product quality is also higher and higher, and meat products is not only needed to have high nutrition, low fat, low cholesterol, green peace Congruent feature, while it is also required to that there is good taste and flavor.Mutton is all the time because of its tender delicious, full of nutrition, wind Taste uniqueness for the advantages that be deeply loved by the public；But mutton has special flavor, that is, the smell of mutton being commonly called as, in different journeys The quality of quality of mutton is affected on degree, this is also the main reason for mutton consumption figure is relatively low.In addition, the raising mould of early weaning Formula also has lamb Slaughter and quality of mutton certain influence, therefore gropes also inquiring into always about yard feeding pattern Among.Based on this, how to improve quality of mutton and flavor is that mutton sheep cultivates faced major issue.
Sensu lato cordyceps sinensis refers to that entomogenous fungi infects the fungus sporophore (son formed after insect or arthropod Seat) and the complex of insect or arthropod.There are about more than 400 to plant for Cordyceps sinensis fungus, wherein most rare is cordyceps sinensis (Cordyceps sinensis) is that the linear Cordyceps sinensis fungus of Clavicipitaceae (Ophiocordyceps) parasitizes bat moth category The complex formed in the larva body of (Hepialus spp.) is the endemic species of Qinghai-Tibet high altitude localities, referred to as " worm Grass " is the rare Chinese medicine of Chinese tradition.
At present, in the world it has been found that the cordyceps sinensis for belonging to " Cordyceps " has as many as 350 kinds, wherein having been sent out in China It now crosses more than 60 to plant, includes cordyceps sinensis (C.sinensis), northern Chinese caterpillar Fungus (C.militaris), agglomerate cordyceps sinensis (C.ophioglossoides), Cordyceps hawkesii Gary (C.hawkesii), Cordyceps gunnii (Berk.) Berk (C.gunnii), coral cordyceps sinensis (martialis), sickle shaped cordyceps sinensis (C.falcata), mountain Tai aweto (C.taishanensis), Shanxi cordyceps sinensis (C.shanxiensis), liangshan cordyceps herb (C.liangshanensis), Xinjiang cordyceps sinensis (C.gracilis), incense stick cordycep (C.barnesii), cicada fungus (C.sobolifera) etc..
Now, it has been found that cordyceps sinensis it is various in style and most cordyceps sinensis plays the role of very strong raising immunocompetence.It is logical Above-mentioned effect is crossed, researchers successively successfully develop：(1) paecilomyces hepiall chen (1987 list), by from Cordyceps of Qinghai Province In separating obtained Paecilomyces hepiali chen (Paecilomyces hepiali) Cs-4 bacterial strains be prepared；(2) Bailing capsule, with A kind of fungi Hirsutella sinensis (also known as Chinese synnema spore (Synnematum sinense that Shen Nanying etc. is isolated in nineteen eighty-three Y in et Shen sp.now), aweto-cephalosporin, Hirsutella hapial) it produces to obtain for strain；(3) zhiling capsules are used The mycelium that mortierella sp (Mortierella sp) fermentation for isolating and purifying out from cordyceps sinensis obtains is made；(4) calming heart Health-care capsule, by Clavicipitaceae fungi cordyceps sinensis cephalo (Cephalosporium sinensis isolated in fresh cordyceps sinensis Chen.sp.nov) through obtained by liquid deep layer fermenting；(5) Xinganbao Jrao nang was isolated by 1986 in cordyceps sinensis fresh specimens The caterpillar fungus cephalosporin come is made, which is a kind of fungi autoeciousness bacterium Gliocladiumroseum [Gliocladium roseum (Link)Bain]。
In addition, application of the worm grass product on animal also obtains some achievements.Mortierella Diding culture has a variety of lifes Object function, such as：Wei Jianzhong etc. is in document《Mortierella Diding culture is to the shadow of child care weaned piglets and immune level It rings》In disclose mortierella Diding culture and can improve the production performance and immune level of child care piglet；And Li Yang etc. is by document 《Effects of acremonium terricola culture on growth performance,antioxidant status and immune functions in weaned calves》、《Effects of acremonium terricola culture supplementation on apparent digestibility,rumen fermentation,and blood parameters in dairy cows》It is published in respectively《Livestock Science》 With《Animal Feed Science and Technology》In, mainly elaborate that Cordyceps strain fermentation object grows calf Influence in terms of performance, cow in milk production performance and immunity.Application publication number is the application for a patent for invention of CN 101797014A A kind of production method of nutritive eggs with low cholesterol and products thereof is elaborated in document；Application publication number is CN 104472904A Application for a patent for invention document in elaborate that Cordyceps strain fermentation object is improving cow's milk flavor substance, vitamin or required amino Application in acid content, application publication number are to elaborate Cordyceps strain in the application for a patent for invention document of CN 104522313A Application of the fermentate in Methods of Milk Somatic Cell Images quantity is reduced.
However, there is presently no the reports applied in terms of quality of mutton is improved about cordyceps sinensis.
Invention content
The present invention provides a kind of method for improving quality of mutton, this method adds using Cordyceps strain fermentation object as feed Agent is added to feed mutton sheep, protein content in mutton can be significantly improved, reduces containing for Boar taint substances in mutton, cholesterol and fat Amount and the pH value and drip loss for reducing mutton, and then realize the raising of quality of mutton.
Particular content is as follows：
A kind of method for improving quality of mutton, including：Mutton sheep is fed using Cordyceps strain fermentation object as feed addictive.
Cordyceps strain fermentation object of the present invention is obtained by Cordyceps sinensis fungus after liquid fermentation or solid fermentation；The worm It can be liquid fermentation bacterium solution or the bacterium powder being prepared into using liquid fermentation solid content that grass, which belongs to strain fermentation object, also It can be the solid fermentation object further prepared using liquid fermentation bacterium solution.
Meaning Cordyceps sinensis fungus of the invention refers to isolated parasitical fungi from cordyceps sinensis.
Further, the Cordyceps sinensis fungus is cordyceps sinensis (C.sinensis) strain, northern Chinese caterpillar Fungus (C.militaris) strain, agglomerate cordyceps sinensis (C.ophioglossoides) strain, Cordyceps hawkesii Gary (C.hawkesii) strain, Cordyceps gunnii (Berk.) Berk (C.gunnii) strain, coral cordyceps sinensis (C.martialis) strain, sickle shaped cordyceps sinensis (C.falcata) strain, Thailand Mountain cordyceps sinensis (C.taishanensis) strain, Shanxi cordyceps sinensis (C.shanxiensis) strain, liangshan cordyceps herb (C.liangshanensis) strain, Xinjiang cordyceps sinensis (C.gracilis) strain, incense stick cordycep (C.barnesii) strain, cicada fungus (C.sobolifera) at least one of strain.
Further, the Cordyceps sinensis fungus is mortierella Diding (Acremonium terricola), Hirsutella sinensis (Synnematium sinense sp.), pupa draw up mould (Paecilomyces militaris), Paecilomyces hawkesii (Paecilomyces hawkesii), Paraisaria mould (Paraisaria sp.), false truffle (Elaphomycesgranulatus), at least one of cicada muscardine (Beauveria sobolifera).
Experiments have shown that compared with the fermentate of other Cordyceps sinensis fungus, mortierella Diding (Acremonium terricola) Quality of mutton can more effectively be improved；Therefore preferably, the Cordyceps sinensis fungus is mortierella Diding (Acremonium terricola)。
Further, the Cordyceps sinensis fungus is mortierella Diding (Acremonium terricola) MKL18；It is true with this Bacterium can significantly improve the quality of mutton as feed addictive.
The liquid fermentation includes：(1) Cordyceps sinensis fungus is activated；(2) Cordyceps sinensis fungus is taken to be inoculated in seed culture In base, seed culture is carried out；It is forwarded to and expands in culture medium again, be enlarged culture, obtain liquid fermentation bacterium solution.
The solid fermentation includes：After liquid fermentation bacterium solution is taken to be mixed with solid medium, fermentation obtains solid culture Object.
The liquid fermentation medium and solid fermentation culture medium that the present invention uses are conventional medium, without special Chinese medicine Ingredients are waited, Cordyceps sinensis fungus and its fermentating metabolism product are only contained in the fermentate of acquisition, the present invention is to utilize Cordyceps sinensis fungus The tunning that itself fermentation generates realizes the raising of quality of mutton.
Above-mentioned conventional medium includes nitrogen source, carbon source and trace element；Wherein, preferably, nitrogen source is dried silkworm chrysalis meal, albumen At least one of peptone, dusty yeast and analysis for soybean powder, carbon source be white granulated sugar, analysis for soybean powder, corn flour, malt flour and rice meal in extremely Few one kind, trace element are MgSO4、KH2PO4、NaH2PO4And VBAt least one of.
Further, it is found through experiment that, the ingredient of Cordyceps strain fermentation object that the fermentation of various conventional culture medium obtains contains Amount is different, and different component contents can influence quality of mutton.
Preferably, in the culture medium of liquid fermentation, the nitrogen source includes the dried silkworm chrysalis meal and 5~20g/L of 3~30g/L Peptone (on the basis of culture medium).
Further, the liquid fermentation includes seed culture and expands to cultivate；In terms of 1L culture mediums, the seed training It supports and the culture medium of expansion culture is：20~60g white granulated sugars, 3~30g dried silkworm chrysalis meals, 5~30g analysis for soybean powder, 5~20g peptones, 3 ~40g dusty yeasts, 0.01~1.0g MgSO4, 0.01~1.0g KH2PO4。
Preferably, in terms of the quality of culture medium, the culture medium of the solid fermentation includes：Corn flour 30~80%, cotton Seed powder 5~20%, wheat wheat-middlings 2~15%, dregs of beans 15~40%, wheat bran 5~20%, magnesium sulfate 0.01~1.0%, zinc sulfate 0.01~1.0%, potassium dihydrogen phosphate 0.01~1.0%, sodium dihydrogen phosphate 0.01~1.0%.
Further, Cordyceps sinensis polysaccharide, adenosine and ergosterol are contained in the Cordyceps strain fermentation object.
The results showed Cordyceps sinensis polysaccharide, adenosine and ergosterol that different types of Cordyceps strain fermentation object obtains Content is different, and then influences the quality of mutton.
In terms of mass fraction, containing 0.5~40% Cordyceps sinensis polysaccharide in the Cordyceps strain fermentation object, 0.005~0.4% Adenosine and 0.001~0.6% ergosterol；Further, it is more containing 2~10% cordyceps sinensis in the Cordyceps strain fermentation object Sugar, 0.005~0.2% adenosine and 0.001~0.3% ergosterol.
Further, the mutton sheep be wean after Xinjiang Small-fat-tail sheep, boer goat, Poll Dorset C1-esteraseremmer-N sheep, Suffolk Sheep, Du Boyang, Charolais, German Mutton Merino etc..
Include Boar taint substances in mutton, protein, cholesterol and fat for characterizing the index of the quality of mutton Content, pH value and drip loss.
Further, the Cordyceps strain fermentation object, which has, improves protein content in mutton, reduces smell of mutton in mutton The function of substance, the pH value of the content of cholesterol and fat and reduction mutton and drip loss.
Wherein, the Boar taint substances include the fatty acids substances such as 4- ethyls octanoic acid, 4- methyl nonanoic acids and 4- methyloctanoic acids.
Preferably, the method includes：After the Cordyceps strain fermentation object is mixed with basal diet, then feed meat Sheep；In terms of the dry weight of the basal diet, the additive amount of the Cordyceps strain fermentation object is 0.005~1%.
In the range of the scale of feeding, mutton sheep can reach best meat；Due to the Cordyceps strain fermentation object pair of the present invention Mutton sheep is simultaneously without any side effects, so any adverse effect will not be generated in itself to mutton sheep by increasing scale of feeding.
Cordyceps strain fermentation object of the present invention can also be mixed separately as feed addictive with other feed addictives It uses；It is better if coordinating rational basal diet formula.
Compared with prior art, beneficial effects of the present invention are：
Present invention discover that feeding mutton sheep using Cordyceps strain fermentation object, protein content in mutton can be significantly improved, The pH value and drip loss of Boar taint substances, the content of cholesterol and fat and mutton in mutton are reduced, and then realizes mutton The raising of quality.
Specific embodiment
The present invention is described in further detail With reference to embodiment.
Embodiment 1
1st, zymocyte liquid
(1) Cordyceps gunnii (Berk.) Berk strain：Mortierella Diding (Acremonium terricola) MKL18 (is purchased from Hefei Mai Keluo Bioengineering Co., Ltd)；
(2) seed culture：
The culture medium of seed culture is：600g white granulated sugars, 300g dried silkworm chrysalis meals, 150g peptones, 200g analysis for soybean powder, 100g ferment Female powder, 10g MgSO4, 10g KH2PO4, it is dissolved in 20L water, natural pH；121 DEG C of high pressure steam sterilization 30min.
The condition of seed fermentation is：Temperature is 25 DEG C, and ventilatory capacity is 1.00L/ (Lmin), and inoculum concentration is 5% (quality point Number), 30L airlift fermentors liquid amount 67%, fermentation time is for 24 hours.
(3) expand culture：
Expanding the culture medium cultivated is：6000g white granulated sugars, 3000g dried silkworm chrysalis meals, 1500g peptones, 2000g analysis for soybean powder, 1000g dusty yeasts, 100g MgSO4, 100g KH2PO4, it is dissolved in 200L water, natural pH；121 DEG C of high pressure steam sterilization 30min.
Expanding the condition cultivated is：Temperature is 25 DEG C, and ventilatory capacity is 1.00L/ (Lmin), and inoculum concentration is 10% (quality Score), 300L airlift fermentors liquid amount 67%, fermentation time is for 24 hours.
After the completion of seed culture and expansion culture, mortierella Diding bacterium solution is obtained.
2nd, zymophyte powder
(1) mortierella Diding bacterium solution obtained as process object, pumps out bacterium solution from fermentation tank using in the present embodiment 1, from The heart detaches, and obtains mycelia and solid content；
(2) above-mentioned mycelia and solid content are collected, heated-air drying is down to 8wt% hereinafter, crushing pack to moisture at 45 DEG C, Sealing, is protected from light storage, obtains zymophyte powder.
3rd, solid culture
(1) by (inoculum concentration 15%, matter after zymocyte liquid obtained in the present embodiment 1 and solid medium stirring inoculation Measure score), tray top fermentation, material thickness 3cm are laid in, constant temperature is 25 DEG C, humidity 70%, and fermentation time is 120h obtains solid culture；
Wherein, the culture medium of solid fermentation is：Corn flour 45%, cotton seed meal 5%, wheat wheat-middlings 4%, dregs of beans 30%, wheat Bran 15.8%, magnesium sulfate 0.05%, zinc sulfate 0.05%, potassium dihydrogen phosphate 0.05%, sodium dihydrogen phosphate 0.05%；Natural pH, 121 DEG C of steam sterilizing 1h.
(2) solid culture is collected, heated-air drying is down to 8wt% hereinafter, crushing pack to moisture at 60 DEG C, sealing, It is protected from light storage.
The component in zymocyte liquid, zymophyte powder and solid culture that the present embodiment prepares is measured, as a result such as Shown in table 1.
Compound mensuration result in 1 mortierella Diding zymocyte liquid of table, zymophyte powder and solid culture
Embodiment 2
The present embodiment ferments using mortierella Diding (Acremonium terricola) MKL18 as fermentation object, obtains Obtain zymocyte liquid, zymophyte powder and solid culture；In addition to the culture medium of the liquid fermentation of use is different from embodiment 1, Remaining content is same as Example 1.
The culture medium of seed culture is：600g white granulated sugars, 550g dusty yeasts, 200g analysis for soybean powder, 10g MgSO4, 10g KH2PO4, it is dissolved in 20L water, natural pH；121 DEG C of high pressure steam sterilization 30min.
Expanding the culture medium cultivated is：6000g white granulated sugars, 5500g dusty yeasts, 2000g analysis for soybean powder, 100g MgSO4, 100g KH2PO4, it is dissolved in 200L water, natural pH；121 DEG C of high pressure steam sterilization 30min.
The component in zymocyte liquid, zymophyte powder and solid culture that the present embodiment prepares is measured, as a result such as Shown in table 2.
Compound mensuration result in 2 mortierella Diding zymocyte liquid of table, zymophyte powder and solid culture
Embodiment 3
The present embodiment ferments using mortierella Diding (Acremonium terricola) MKL18 as fermentation object, obtains Obtain zymocyte liquid, zymophyte powder and solid culture；In addition to the culture medium of the liquid fermentation of use is different from embodiment 1, Remaining content is same as Example 1.
The culture medium of seed culture is：600g white granulated sugars, 400g dusty yeasts, 200g analysis for soybean powder, 150g peptones, 10g MgSO4, 10g KH2PO4, it is dissolved in 20L water, natural pH；121 DEG C of high pressure steam sterilization 30min.
Expanding the culture medium cultivated is：6000g white granulated sugars, 4000g dusty yeasts, 2000g analysis for soybean powder, 1500g peptones, 100g MgSO4, 100g KH2PO4, it is dissolved in 200L water, natural pH；121 DEG C of high pressure steam sterilization 30min.
The component in zymocyte liquid, zymophyte powder and solid culture that the present embodiment prepares is measured, as a result such as Shown in table 3.
Compound mensuration result in 3 mortierella Diding zymocyte liquid of table, zymophyte powder and solid culture
Embodiment 4
The present embodiment ferments using mortierella Diding (Acremonium terricola) MKL18 as fermentation object, obtains Obtain zymocyte liquid, zymophyte powder and solid culture；In addition to the culture medium of the liquid fermentation of use is different from embodiment 1, Remaining content is same as Example 1.
The culture medium of seed culture is：600g white granulated sugars, 250g dusty yeasts, 200g analysis for soybean powder, 300g dried silkworm chrysalis meals, 10g MgSO4, 10g KH2PO4, it is dissolved in 20L water, natural pH；121 DEG C of high pressure steam sterilization 30min.
Expanding the culture medium cultivated is：6000g white granulated sugars, 2500g dusty yeasts, 2000g analysis for soybean powder, 3000g dried silkworm chrysalis meals, 100g MgSO4, 100g KH2PO4, it is dissolved in 200L water, natural pH；121 DEG C of high pressure steam sterilization 30min.
The component in zymocyte liquid, zymophyte powder and solid culture that the present embodiment prepares is measured, as a result such as Shown in table 4.
Compound mensuration result in 4 mortierella Diding zymocyte liquid of table, zymophyte powder and solid culture
Component (Cordyceps sinensis polysaccharide, adenosine by table 1~4 it is found that different culture media ferments in the mortierella Diding fermentate obtained With ergosterol) content is different, wherein, Cordyceps sinensis polysaccharide, gland in the fermentate obtained using the liquid fermentation medium of embodiment 1 The equal highest of the content of glycosides and ergosterol.
Embodiment 5
The present embodiment draws up mould (Paecilomyces militaris) MKL32 with pupa and (is purchased from Anhui nova biology Engineering Co., Ltd) it ferments as fermentation object, obtain zymocyte liquid, zymophyte powder and solid culture；Preparation side Method is same as Example 1.
The component in zymocyte liquid, zymophyte powder and solid culture that the present embodiment prepares is measured, as a result such as Shown in table 5.
5 pupa of table draws up the compound mensuration result in mould zymocyte liquid, zymophyte powder and solid culture
Embodiment 6
The present embodiment (is purchased from Anhui nova biology with Hirsutella sinensis (Synnematium sinense sp.) MKL25 Engineering Co., Ltd) it ferments as fermentation object, obtain zymocyte liquid, zymophyte powder and solid culture；Preparation side Method is same as Example 1.
The component in zymocyte liquid, zymophyte powder and solid culture that the present embodiment prepares is measured, as a result such as Shown in table 6.
Compound mensuration result in 6 Hirsutella sinensis zymocyte liquid of table, zymophyte powder and solid culture
Component (Cordyceps sinensis polysaccharide, adenosine and ergot steroid by table 1,5 and 6 it is found that different strain ferments in the fermentate obtained Alcohol) content is also different；Wherein, the fermentation obtained using the mortierella Diding of embodiment 1 (Acremonium terricola) MKL18 The content highest of Cordyceps sinensis polysaccharide, adenosine and ergosterol in object.
Embodiment 7
1st, test process：
Test plan is carried out in the large-scale mutton sheep cultivation base in Hainan, is adopted in -2017 years on the 3rd July in 2017 of August 30 For the Cordyceps strain fermentation object prepared by the use of Examples 1 to 6 as feed addictive, wherein 3-July 10 July is preliminary trial period, 50 days are experimental period afterwards.
It is as follows to test particular content：
350 hairs is selected to educate the Small-fat-tail sheep that normal, weight approaches and is in a good state of health, weight 10kg ± 0.5kg, with Machine is divided into 7 groups (test group 1~6 and control groups), respectively：
Control group：Basal diet is only fed, the composition of basal diet is shown in Table 7；
Test group：Respectively with embodiment 1 (test group 1), embodiment 2 (test group 2), embodiment 3 (test group 3), implementation The Cordyceps strain solid culture that example 4 (test group 4), embodiment 5 (test group 5) and embodiment 6 (test group 6) prepare For feed addictive, it is added in basal diet, obtained feed feeding sheep；In terms of the quality of basal diet, in preliminary trial period Interior, the additive amount of each Cordyceps strain solid culture is 0.1%；Within experimental period, each Cordyceps strain solid culture Additive amount is 0.2%.
7 basal diet of table forms and trophic level (air bells dry basis)
2nd, the acquisition of index and detection method
During off-test, test group and each random each 5 progress of the examination sheep for selecting weight and being in a good state of health of control group It butchers, weighs before butchering, water 16h is prohibited in fasting, and next day takes jugular vein bloodletting hair to be butchered.
It butchers rear every examination sheep and takes eye muscle (longissimus dorsi muscle between the 1st and the 2nd rib cage) and gluteus, measure following index：
(1) drip loss：2 pieces of eye muscle is taken, length × width x thickness is 5cm × 3cm × 2cm, hangs on disposable paper respectively In cup and mutton sheep does not contact with wall of cup, is placed in 4 DEG C of refrigerators, taking-up blots surface moisture with blotting paper and weighs afterwards for 24 hours：
Drip loss (%)=100 × (first sample weight-last sample weight)/first sample weight,
(2) Boar taint substances, cholesterol, fat, the content of protein and pH value (acidometer PHS-2 types) in mutton.
The assay method of cholesterol：Mutton sheep grinding is acquired, 4 DEG C of refrigerated centrifuge, 3000r/min, centrifugation 20min are discarded Upper-layer fat and the precipitation at centrifuge tube bottom, take central, clear liquid to be measured with RNA isolation kit；Fat：GB/T6433-1994 Soxhlets are taken out Formulation；Protein：GB/T6432-1994 Kjeldahl's methods.
Boar taint substances assay method：Thermo ISQ1301, gas chromatograph for determination.
3rd, result of the test
Concrete outcome is shown in Table shown in 8~9.
Mutton Boar taint substances in 8 different tests group of table, cholesterol, fat, protein, pH value and drip loss situation
As shown in Table 8, compared with the control group, test group 1~6 significantly reduces Boar taint substances in mutton sheep, cholesterol, fat Content and pH value and drip loss, significantly improve the content of protein；Wherein, the best results of test group 1.Thus it says It is bright：Using different culture media and different strain carry out the solid culture of fermentation acquisition in terms of improve quality of mutton on exist Difference, and above-mentioned difference is likely to be constituent content difference (especially Cordyceps sinensis polysaccharide, adenosine and ergosterol in fermentate Content) caused by.

Claims (9)

  1. A kind of 1. method for improving quality of mutton, which is characterized in that including：Using Cordyceps strain fermentation object as feed addictive Feed mutton sheep.
  2. 2. the method as described in claim 1, which is characterized in that the Cordyceps strain fermentation object is by Cordyceps sinensis fungus through liquid It is obtained after fermentation or solid fermentation.
  3. 3. method as claimed in claim 2, which is characterized in that the Cordyceps sinensis fungus is cordyceps sinensis (C.sinensis) bacterium Kind, northern Chinese caterpillar Fungus (C.militaris) strain, agglomerate cordyceps sinensis (C.ophioglossoides) strain, Cordyceps hawkesii Gary (C.hawkesii) strain, Cordyceps gunnii (Berk.) Berk (C.gunnii) strain, coral cordyceps sinensis (C.martialis) strain, sickle shaped cordyceps sinensis (C.falcata) strain, mountain Tai aweto (C.taishanensis) strain, Shanxi cordyceps sinensis (C.shanxiensis) strain, the Liangshan Mountain Cordyceps sinensis (C.liangshanensis) strain, Xinjiang cordyceps sinensis (C.gracilis) strain, incense stick cordycep (C.barnesii) strain, At least one of cicada fungus (C.sobolifera) strain.
  4. 4. method as claimed in claim 3, which is characterized in that the Cordyceps sinensis fungus is mortierella Diding (Acremonium Terricola), Hirsutella sinensis (Synnematium sinense sp.), pupa draw up mould (Paecilomyces Militaris), Paecilomyces hawkesii (Paecilomyces hawkesii), Paraisaria mould (Paraisaria sp.), big At least one of group capsule bacterium (Elaphomyces granulatus), cicada muscardine (Beauveria sobolifera).
  5. 5. method as claimed in claim 2, which is characterized in that the Cordyceps sinensis fungus is mortierella Diding (Acremonium terricola)。
  6. 6. method as claimed in claim 2, which is characterized in that the culture medium of the liquid fermentation includes：Nitrogen source, carbon source and micro- Secondary element；
    Wherein, nitrogen source is at least one of for dried silkworm chrysalis meal, peptone, dusty yeast and analysis for soybean powder, carbon source is white granulated sugar, analysis for soybean powder, At least one of corn flour, malt flour and rice meal, trace element are MgSO4、KH2PO4、NaH2PO4And VBIn at least one Kind.
  7. 7. method as claimed in claim 2, which is characterized in that in terms of mass fraction, contain in the Cordyceps strain fermentation object There are 0.5~40% Cordyceps sinensis polysaccharide, 0.005~0.4% adenosine and 0.001~0.6% ergosterol.
  8. 8. the method as described in claim 1, which is characterized in that the index for characterizing the quality of mutton includes：In mutton Boar taint substances, protein, cholesterol and fat content, pH value and drip loss.
  9. 9. the method as described in claim 1, which is characterized in that mix the Cordyceps strain fermentation object with basal diet Afterwards, then mutton sheep is fed；
    In terms of the dry weight of the basal diet, the additive amount of the Cordyceps strain fermentation object is 0.005~1%.
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