CN104745494A - Pig bacillus coagulans preparation used for substitution of antibiotics and improvement of intestinal micro-ecological environment and preparation method thereof - Google Patents
Pig bacillus coagulans preparation used for substitution of antibiotics and improvement of intestinal micro-ecological environment and preparation method thereof Download PDFInfo
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- CN104745494A CN104745494A CN201310744727.6A CN201310744727A CN104745494A CN 104745494 A CN104745494 A CN 104745494A CN 201310744727 A CN201310744727 A CN 201310744727A CN 104745494 A CN104745494 A CN 104745494A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
Abstract
The invention belongs to the technical field of microbial application, and in particular relates to a pig bacillus coagulans preparation used for substitution of antibiotics and improvement of intestinal micro-ecological environment and a preparation method thereof. The pig bacillus coagulans preparation is mainly characterized in that: the bacillus coagulans PFK1102A is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 24, 2012, and the preservation number is CGMCCNO.6047. By comprehensive compatibility and combined use of the bacillus coagulans PFK1102A, an intestinal energy providing material and an enzyme preparation, the effects of promoting animal intestinal development, enhancing animal immunity, substituting or reducing the use of the antibiotics, improving the intestinal micro-ecological environment, and meeting the intestinal nutrition physiology and nutrition requirement dynamic variation.
Description
Technical field
The invention belongs to technical field of microbe application, be specifically related to a kind of for substitute antibiotics and the pig Bacillus coagulans preparation improving intestine microenvironment and preparation method thereof.
Background technology
Since nearly half a century, microbiotic adds in feed as antibacterial growth substance and is widely used, and microbiotic, to the generation controlling livestock and poultry, promotes animal growth and development, improves raise benefit and really serves positive effect.But widely use along with antibiotic, people recognize that it also exists some drawbacks gradually, mainly contain following problem:
Antibiotic use destroys original microbial balance in animal body, harmful pathogenic micro-organism is there is in animal body, also there is the microorganism that some are useful, microbiotic is also killed and is inhibit useful microorganism while killing pathogenic micro-organism in animal body and suppressing, and has upset the general layout of restriction mutually between microorganism species, destroy the eubiosis of microorganism, thus cause the disorders of digestion, cause various digestive tract diseases, as chronic diarrhoea etc.; Life-time service microbiotic can make pathogenic micro-organism develop immunity to drugs, and after these have drug-fast pathogenic micro-organism amount reproduction, animal suffering from disease chance is increased, and bring difficulty to the treatment of disease, even make microbiotic ineffective, cause mortality of animals to increase, cause cultivation loss; In animal body, have one period of residence time after microbiotic metabolism, if now eaten by people, then can affect body immunity and disease resistance, long-term taking can cause immune function of human body disorderly, in Disease epizootic, bury huge hidden danger etc.
For this reason, many countries have released one after another and have limited or forbid in feed, add antibiotic a series of compulsory measure; Along with the develop rapidly of China's livestock industry, microbiotic problem also progressively comes into one's own, China has also progressively put into effect some measures and has added antibiotic kind and addition in limiting feed, therefore " nonreactive " feed will become the megatrend of following animal husbandry development, and the research and development of Substitutes For Antibiotic have become the focus of livestock development.
Now there are some researches show, adding useful exogenous microbial by increasing the probiotics in digestive tube, suppressing harmful bacteria, adjustment or maintenance intestinal microflora balance, reach preventing disease, growth promoting effects, improve the effect of efficiency of feed utilization.Therefore inoculating microbe has become research hot topic as Substitutes For Antibiotic.But single use inoculating microbe is as Substitutes For Antibiotic, the object maintaining animal intestinal health can not be reached.
The present invention is directed to above-mentioned situation, develop one and can substitute or reduce antibiotic usage, the pig Bacillus coagulans preparation that animal intestinal is grown, improved immunologic function, nutritional-physiological and nutritional needs dynamic variation can be met again.
Summary of the invention
The object of the invention is that providing a kind of substitutes or reduce microbiotic, and intestinal microflora balance can be improved, strengthen animal immune function, improve animal intestinal health and nutrition absorption efficiency, reduce the pig Bacillus coagulans preparation of nitrogen, phosphorus and obnoxious flavour content in movement.
Technical scheme of the present invention is:
Substitute antibiotics and improve the Bacillus coagulans preparation of intestine microenvironment, its moiety and weight percent are:
Bacillus coagulans PFK1102A 60 ~ 90%;
Glutaminase 0.5 ~ 5%;
L-glutamic acid 0.5 ~ 5%;
Aspartic acid 1 ~ 5%;
Mannase 0.5 ~ 5%;
Proteinase-10 .5 ~ 5%;
Thermostableα-amylase 0.3 ~ 5%;
Aspartic protease 0.3 ~ 5%;
Beta-glucanase 0.3 ~ 3%;
Cellulase 0.3 ~ 3%;
Phytase 0.2 ~ 3%;
Surplus is pharmaceutically acceptable auxiliary material.
The weight percent of moiety described in above-mentioned Bacillus coagulans preparation is preferably: Bacillus coagulans PFK1102A80%, glutaminase 3%, L-glutamic acid 3%, aspartic acid 2.5%, mannase 2%, proteolytic enzyme 2%, thermostableα-amylase 1.5%, aspartic protease 1.5%, beta-glucanase 1.5%, cellulase 1.5%, phytase 1%, surplus is pharmaceutically acceptable auxiliary material.
Described auxiliary material is at least one in the auxiliary materials such as zeolite powder, Zulkovsky starch, sucrose or lactose.
Bacillus coagulans PFK1102A, glutaminase, L-glutamic acid, aspartic acid, mannonase proteolytic enzyme, thermostableα-amylase, aspartic protease, beta-glucanase, cellulase, phytase and auxiliary material is taken according to aforementioned proportion, mix, i.e. obtained Bacillus coagulans preparation of the present invention.
Glutaminase, L-glutamic acid, aspartic acid and auxiliary material described in above-mentioned Bacillus coagulans preparation, all can buy on market.As bought from international ingredient companies of the U.S..
Zymin described in above-mentioned Bacillus coagulans preparation and phytase, can buy on market.As (China) Investment Co., Ltd can be believed from Novi or buy from GuangDong YiDuoLi Biology Science Co., Ltd.
The using method of above-mentioned Bacillus coagulans preparation, above-mentioned probiotics joins in pig feed by the weight percent according to 0.03 ~ 0.15%, mixes.
Wherein, Bacillus coagulans PFK1102A it both possessed that lactobacillus is acidproof, anaerobic metabolism, lactic acid producing all excellent characteristics, possess again the features such as genus bacillus is high temperature resistant, acidproof, have and reduce Animal stress reaction, diseases prevention, health care, growth promoting effects, improve the effects such as breeding environment; And the Pfansteihl of metabolism output during the fermentation, do not produce the material that human body, cultivated animals are harmful to, and there is nontoxic, noresidue, the feature such as have no side effect, and can be widely used in high yield, high-quality, efficient, ecological, safe livestock and poultry cultivation eco-environmental control; Bacillus coagulans PFK1102A or a kind of environmentally friendly microorganism simultaneously, can not to environment.
The main energy supply material of small intestine is glutamine, L-glutamic acid, aspartic acid.Feed diet GLN, L-glutamic acid and aspartic acid 90% is utilized by enteron aisle, wherein 50%-70% is for being oxidized energy supply, if lack, hypoalimentation or all can cause that Small Intestine of Piglets and mucous membrane weight reduce, height of naps declines by enteral nutrition level deficiency, protein and DNA synthesis capability reduces, bacterial translocation.
Fodder enzyme preparation, as the probiotics of a kind of efficient, environmental protection, safety, can supplement the deficiency of animal endogenous enzyme, can eliminate and reduce the undesirable action of antinutritional factor in feed, raising efficiency of feed utilization, growth promoting effects.Nitrogen, phosphorus are the main nutrient elements of growth of animals or poultry, are also resource scarcitys on the one hand at present, but utilize insufficient, excessive excretion to cause two kinds of elements of severe environmental pollution on the other hand.Phytase can not only phytic acid in hydrolysate feed and its esters and improve the utilization ratio of protein and the mineral element such as phosphorus, calcium, and can improve the potential nutritive value of feed, thus reaches saving feed resource, reduces feed cost.
The present invention, by the comprehensive compatible combination of Bacillus coagulans PFK1102A, enteron aisle energy supply material, zymin, reaches the effect substituting or reduce antibiotic usage amount, promote that animal intestinal is grown, improve immunologic function, meet nutritional-physiological and nutritional needs dynamic variation; Wherein Bacillus coagulans PFK1102A energy lactic acid producing, can also secrete a large amount of enzymes, can improve the activity of enzyme; Simultaneously, glutamine, L-glutamic acid and aspartic acid can meet the lasting energy supply of enteron aisle, for probiotics provides power endlessly in the field planting of enteron aisle and product acid, thus reach and improve animal intestinal health, improve nutrition absorption efficiency, reduce the object of nitrogen, phosphorus and obnoxious flavour content in movement.
Compared with prior art, tool of the present invention has the following advantages: (1) the present invention is alternative or reduce antibiotic usage quantity, is conducive to the microbiotic foundation of animal intestinal and maintenance; (2) the present invention can meet the lasting energy supply of enteron aisle, for probiotics provides power endlessly in the field planting of enteron aisle and product acid; (3) the present invention is ecological, safe, without any side effects.
Embodiment
Based on embodiment, the present invention is further illustrated below, but do not form any restriction to the present invention.
The preparation of embodiment 1 Bacillus coagulans preparation of the present invention
Each component is got: Bacillus coagulans PFK1102A80%, glutaminase 3%, L-glutamic acid 3%, aspartic acid 2.5%, mannase 2%, proteolytic enzyme 2%, thermostableα-amylase 1.5%, aspartic protease 1.5%, beta-glucanase 1.5%, cellulase 1.5%, phytase 1%, Zulkovsky starch 0.5% according to following ratio; Put into agitator after proportionally being taken by above-mentioned material again and be fully uniformly mixed to obtain Bacillus coagulans preparation of the present invention.
Test example 1 Bacillus coagulans preparation of the present invention substitute antibiotics is on the impact of Growth Performance of Weaning Piglets and intestinal microflora
(1) feed formulation
Control group feed: existing common cooperation weaning pigs, its material composition and weight percent are: corn 63.065%, soybean meal 26.00%, import steam fish meal 3.00%, whey powder 2.00%, soybean oil 2.00%, secondary calcium phosphate 1.50%, stone flour 1.00%, salt 0.5%, mineral substance premix 0.15%, vitamin premix 0.04%, olaquindox 0.015%, kitasamycin 0.01%, colistine sulfate 0.02%, Methionin 0.40%, Threonine 0.20%, methionine(Met) 0.10%.Put into agitator again after proportionally being taken by above-mentioned material to be fully uniformly mixed.
Test group feed: the Bacillus coagulans preparation of embodiment 1 is substituted olaquindox, its material composition and weight percent are: corn 63.00%, soybean meal 26.00%, import steam fish meal 3.00%, whey powder 2.00%, soybean oil 2.00%, secondary calcium phosphate 1.50%, stone flour 1.00%, salt 0.5%, mineral substance premix 0.15%, vitamin premix 0.04%, execute the Bacillus coagulans preparation 0.08% of example 1, kitasamycin 0.01%, colistine sulfate 0.02%, Methionin 0.40%, Threonine 0.20%, methionine(Met) 0.10%.Put into agitator again after proportionally being taken by above-mentioned material to be fully uniformly mixed.
(2) test grouping and method:
Test grouping: Du × length × Dasanyuan hybridization piglet 80 selecting 28 age in days wean, is divided into 2 process (male or female, P>0.05) at random by body weight, each process 5 hurdle (every hurdle is a repetition), 8, every hurdle.
Feeding and management and diseases prevention and treatment: the method that various feeding and management and diseases prevention and treatment provide by " Beijing six horse standard raise pigs pattern " (Chinese agriculture press, 2006.8) is carried out.Be fed to May from March, 35 days trial periods, respectively empty stomach carried out to every piglet when on-test (the 1st day) and end (the 35th day) and weigh, record charging capacity, clout amount and waste, calculate feed-weight ratio according to average daily gain and average daily ingestion amount; Every day trial period, 7:30-19:00 observed the defecation situation of piglet, ight soil is comparatively sticky, shapeless, comparatively juicy person count diarrhoea, total head number × 100% of diarrhea rate=diarrhoea head number/piglet; Extract 2 pig blood samplings on every hurdle in units of repeating in 35d morning of test, after centrifugal, preserve glucose, total protein, albumin, triglyceride level, serum urea nitrogen, glutamic-oxal(o)acetic transaminase and gpt content in test serum for-20 DEG C; In units of repeating, the fresh excrement sample of piglet discharge is collected morning at the 35d of test, encapsulate with aseptic plastic bag, in aseptic operating platform, accurately take excrement sample 1g, be put in the triangular flask filling 99 mL sterile salines, fully vibrate, get the doubling dilution that diluent carries out 10 times, drawing 0.1 mL diluent is inoculated on selective medium, carries out enumeration to intestinal bacteria, lactobacillus, bifidus bacillus.
(3) test-results is as follows: (table 1, table 2, table 3)
Table 1 Bacillus coagulans preparation of the present invention substitute antibiotics is on the impact of Growth Performance of Weaning Piglets
From table 1, part microbiotic in the alternative diet of Bacillus coagulans preparation of the present invention, the heavy control group in the average end of test group test pig improves 0.13kg; Test group and control group diarrhea rate maintain an equal level, and are 0.60%; The survival rate of test group and control group is 100%.
Table 2 Bacillus coagulans preparation of the present invention substitute antibiotics is on the impact of weanling pig Biochemical Indices In Serum
From table 2, test group and control group glucose in serum, total protein, albumin, triglyceride level, serum urea nitrogen, glutamic-oxal(o)acetic transaminase and gpt difference significantly (P>0.05), do not illustrate part microbiotic in the alternative diet of Bacillus coagulans preparation of the present invention.
Table 3 Bacillus coagulans preparation of the present invention substitute antibiotics is on the impact of weanling pig intestinal microecology.
From table 3, Bacillus coagulans preparation of the present invention substitutes part microbiotic in diet, and test group intestinal bacteria quantity has a declining tendency, the trend that lactobacillus and bifidobacteria are improved, and is more conducive to the balance of intestinal microecology and maintains intestinal health.
Claims (4)
1. the excellent Bacillus coagulans of a strain pig, it is characterized in that: described Bacillus coagulans PFK1102A is that a strain had both possessed that lactobacillus is acidproof, anaerobic metabolism, lactic acid producing all excellent characteristics, possess again genus bacillus high temperature resistant, acidproof, carry out the feature such as choline, long preservative period, have and reduce Animal stress reaction, diseases prevention, health care, growth promoting effects, improve the effects such as breeding environment; This bacterial strain on April 24th, 2012 in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number: CGMCC NO.6047.
2. an application for Bacillus coagulans, is characterized in that: the comprehensive compatible combination of Bacillus coagulans PFK1102A, enteron aisle energy supply material, zymin.
3. the boar preparation of Bacillus coagulans preparation, its moiety and weight percent are: Bacillus coagulans PFK1102A60 ~ 90%; Glutaminase 0.5 ~ 5%; L-glutamic acid 0.5 ~ 5%; Aspartic acid 1 ~ 5%; Mannase 0.5 ~ 5%; Proteinase-10 .5 ~ 5%; Thermostableα-amylase 0.3 ~ 5%; Aspartic protease 0.3 ~ 5%; Beta-glucanase 0.3 ~ 3%; Cellulase 0.3 ~ 3%; Phytase 0.2 ~ 3%; Surplus is pharmaceutically acceptable auxiliary material.
4. pig according to claim 3 Bacillus coagulans preparation, it is characterized in that, described optimum weight per-cent is preferably: Bacillus coagulans PFK1102A80%, glutaminase 3%, L-glutamic acid 3%, aspartic acid 2.5%, mannase 2%, proteolytic enzyme 2%, thermostableα-amylase 1.5%, aspartic protease 1.5%, beta-glucanase 1.5%, cellulase 1.5%, phytase 1%, surplus is pharmaceutically acceptable auxiliary material.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105306060A (en) * | 2015-12-03 | 2016-02-03 | 湖南先步信息股份有限公司 | Anti-interference high-precision analog quantity sampling method and apparatus |
| CN107686826A (en) * | 2017-11-08 | 2018-02-13 | 武汉轻工大学 | The preparation method and pig feed of a kind of bacillus coagulans and its fermentation process, bacillus coagulans preparation |
| CN109619300A (en) * | 2018-12-24 | 2019-04-16 | 江西恒牧科技发展有限公司 | A kind of sucking pig takes orally iron supplementary and preparation method thereof |
| CN115074281A (en) * | 2022-06-27 | 2022-09-20 | 华中农业大学 | Bacillus ginseng strain capable of highly producing organic acid and application thereof in microecological preparation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101544993A (en) * | 2009-01-21 | 2009-09-30 | 江苏省苏微微生物研究有限公司 | Method for producing L-lactic acid by Bacillus coagulans CGMCC No.2602 |
| CN103349186A (en) * | 2013-08-01 | 2013-10-16 | 正大康地（澄海）有限公司 | Weaned piglet feed additive and preparation method thereof |
| CN103589654A (en) * | 2012-08-14 | 2014-02-19 | 湖南普菲克生物科技有限公司 | Bacillus coagulans strain and fast identification of anaerobic metabolism and lactic acid production of the strain in animal digestive tract hindgut segment simulator |
-
2013
- 2013-12-31 CN CN201310744727.6A patent/CN104745494A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101544993A (en) * | 2009-01-21 | 2009-09-30 | 江苏省苏微微生物研究有限公司 | Method for producing L-lactic acid by Bacillus coagulans CGMCC No.2602 |
| CN103589654A (en) * | 2012-08-14 | 2014-02-19 | 湖南普菲克生物科技有限公司 | Bacillus coagulans strain and fast identification of anaerobic metabolism and lactic acid production of the strain in animal digestive tract hindgut segment simulator |
| CN103349186A (en) * | 2013-08-01 | 2013-10-16 | 正大康地（澄海）有限公司 | Weaned piglet feed additive and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 余华: "仔猪断奶应激与调控应激的途径探讨", 《中国畜牧兽医学会动物微生态学分会第三届第八次学术研讨会论文集（2006年广州）》 * |
| 周映华 等: "不同芽孢杆菌对断奶仔猪生产性能的影响", 《饲料工业》 * |
| 张高娜 等: "凝结芽孢杆菌的特性及其研究进展", 《饲料与畜牧》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105306060A (en) * | 2015-12-03 | 2016-02-03 | 湖南先步信息股份有限公司 | Anti-interference high-precision analog quantity sampling method and apparatus |
| CN107686826A (en) * | 2017-11-08 | 2018-02-13 | 武汉轻工大学 | The preparation method and pig feed of a kind of bacillus coagulans and its fermentation process, bacillus coagulans preparation |
| CN107686826B (en) * | 2017-11-08 | 2022-07-12 | 武汉轻工大学 | Bacillus coagulans and fermentation method thereof, preparation method of bacillus coagulans preparation and pig feed |
| CN109619300A (en) * | 2018-12-24 | 2019-04-16 | 江西恒牧科技发展有限公司 | A kind of sucking pig takes orally iron supplementary and preparation method thereof |
| CN115074281A (en) * | 2022-06-27 | 2022-09-20 | 华中农业大学 | Bacillus ginseng strain capable of highly producing organic acid and application thereof in microecological preparation |
| CN115074281B (en) * | 2022-06-27 | 2023-10-10 | 华中农业大学 | Ginseng bacillus capable of producing organic acid at high yield and application of ginseng bacillus in microecological preparation |
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Application publication date: 20150701 |