CN106306358A - Formula and production technology of fermentation traditional Chinese medicine feed additive - Google Patents
Formula and production technology of fermentation traditional Chinese medicine feed additive Download PDFInfo
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Abstract
The invention provides a fermentation traditional Chinese medicine feed additive and a production technology thereof, and relates to the field of feed additives. The formula of the fermentation traditional Chinese medicine feed additive consists of the following raw materials in parts by weight: 10-20 parts of wild chrysanthemums, 10-20 parts of glossy privet fruits, 10-20 parts of haws, 10-20 parts of motherwort, 10-20 parts of folium isatidis, 10-20 parts of honeysuckle stems, 10-20 parts of radix scutellariae, and 2-10 parts of liquid spawn. According to the fermentation traditional Chinese medicine feed additive disclosed by the invention, the specificity and the nonspecific immunity of animal organisms can be effectively improved, the disease resistance of the organisms can be strengthened, the dosage during a breeding period is reduced, and the drug residues are reduced; balance of gastrointestinal bacterial flora of animals is adjusted, comprehensive absorption of nutrients is facilitated, and the breeding cost such as the feed conversion and the feed-to-egg ratio is reduced; the breeding environment is improved, harmful gas such as ammonia is reduced, mycotoxin is removed, and damage caused by mildew and rot of the feed is reduced; and the quality of animal products is improved, and the breeding benefits are increased.
Description
Technical field
The present invention relates to feed additive field, concrete finger one fermented tcm feed addictive prescription and production work thereof
Skill.
Background technology
Along with the development of society, the continuous of population increases, more and more higher to the demand of social materials nutrition.As human body
Necessary nutrition, protein is necessary to maintenance people survive orthobiosis in other words.In life in the source of protein, domestic animal
Poultry or poultry are as the main source of protein.It is apparent that in the face of the hugest demand, if simple dependence nature
Put in a suitable place to breed and can not meet people's demand to protein at all.
Therefore, cultivation batch production slowly becomes provides the main solution of people's protein requirement.But along with supporting
Grow batch production and come, owing in plant, animal consumption nutrient is single, either with or without enough activity spaces, therefore,
It is single often to there is nutrient in the protein product of standardization cultivation, or due to the various additive even right and wrong not conforming to specification
The use of method additive causes protein product can not meet the demand of people.Further, due to the battalion of Long term Animal in plant
Support Deficiency of Intake, also result in growth rate slow, the appearance of the problems such as body constitution is weak, the most sick.
Therefore it provides a kind of feed additive that can solve the problem that the problems referred to above has higher economic worth and society's valency
Value.
Summary of the invention
In order to solve the problems referred to above, the present invention provides a kind of fermented tcm feed addictive prescription and production technology thereof.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of fermented tcm feed addictive prescription, is made up of by weight following raw materials: Flos Chrysanthemi Indici 10-20 part, Fructus Ligustri Lucidi
Sub-10-20 part, Fructus Crataegi 10-20 part, Herba Leonuri 10-20 part, Folium Isatidis 10-20 part, Caulis Lonicerae 10-20 part, Radix Scutellariae 10-20 part,
Liquid spawn 2-10 part.
As preferably, a kind of fermented tcm feed addictive prescription, it is made up of by weight following raw materials: Herba Dendranthematis indici
Spend 15 parts, Fructus Ligustri Lucidi 15 parts, Fructus Crataegi 15 parts, Herba Leonuri 15 parts, Folium Isatidis 15 parts, Caulis Lonicerae 15 parts, Radix Scutellariae 15 parts, liquid spawn
5 parts.
Further, described liquid spawn is made up of by weight following raw materials: Enterococcus faecalis fermentation liquid 1-1000
Part, bacillus subtilis 0.1-1 part, Bacillus licheniformis 0.1-1 part, Clostridium butyricum 0.1-1 part, saccharomyces cerevisiae 0.1-1 part, Portugal
Grape sugar 20-30 part, cold water 1-1000 part.
Further, the preparation method of described Enterococcus faecalis fermentation liquid is:
(1) on one-level solid medium, enterococcus faecalis is inoculated, according to strain: solid medium is 1-2ml:100-by volume
Ratio inoculation (wherein strain concentration is 1-10 hundred million/ml) of 150ml, cultivates 18-24 hour, obtains primary cultivation for 35-37 DEG C
(described solid culture based raw material and preparation method be strain: takes peptone 0.5 1.5% yeast powder 0.3-by weight proportion
1.0% lactose 1-2%, agar powder 1.5-2.0%, surplus is water, and above-mentioned each raw material is made aqueous solution, adjusts PH to 6.5-7.5,
Subpackage in eggplant-shape bottle, 115 DEG C of sterilizings 30 minutes, is placed and is i.e. obtained solid medium)；
(2) primary strain of cultivating that step (1) obtains is taken according to strain: fluid medium connects by weight the ratio of 0.5:100
Plant in fluid medium, cultivate 8-16 hour, during wherein fluid medium is placed on fermentation tank, keep tank pressure for 35-37 DEG C
0.03 ± 0.01MPa, (described liquid medium starting material and preparation method are to take by weight proportion to obtain secondary cultivation strain
Peptone 1.0-2.5%, yeast powder 0.3-1.0%, glucose 1.0-3.0%；Sodium acetate 0.2-0.7%, potassium dihydrogen phosphate 0.2-
0.5%, ammonium citrate 0.2-0.5%, surplus is water, and above-mentioned each raw material is made aqueous solution, adjusts PH to 6.5-7.5,100-121
DEG C sterilizing 15-20 minute, is cooled to 35-37 DEG C and i.e. obtains fluid medium)；
(3) the secondary strain of cultivating step (2) obtained cultivates strain according to secondary: fermentation medium is by weight 10:100's
Ratio is inoculated in fermentation medium, cultivates 14-24 hour for 35-37 DEG C, during wherein fluid medium is placed on fermentation tank, keeps tank
Pressure 0.03 ± 0.01MPa, (described fermentation culture based raw material and preparation method are, by weight to obtain Enterococcus faecalis fermentation liquid
Example takes peptone 1.0-4.0%, yeast powder 0.3-1.0%, glucose 1.0-5.0%；Sodium acetate 0.2-0.7%, potassium dihydrogen phosphate
0.2-0.5%, ammonium citrate 0.2-0.5%, calcium carbonate 0.6-1.6%, surplus is water, and above-mentioned each raw material is made aqueous solution, adjusts
PH to 6.5-7.5,100-121 DEG C of sterilizing 15-20 minute, is cooled to 35-37 DEG C and i.e. obtains fermentation medium).
A kind of fermented tcm feed addictive prescription and production technology thereof, described production technology is for take phase according to above-mentioned formula
Answer the Flos Chrysanthemi Indici of weight, Fructus Ligustri Lucidi, Fructus Crataegi, Herba Leonuri, Folium Isatidis, Caulis Lonicerae, Radix Scutellariae, each component is inserted grinder-mixer
In, it is pulverized and mixed uniformly, adds the liquid spawn of corresponding weight, mix homogeneously, subpackage is to the one-way breather valve fermentation bag sealed
In, 30-37 DEG C ferments 48-120 hour, gets product.
The present invention also includes the application in field of fodder of the fermented tcm feed additive, and application process is according to 1000-
The ratio of 3000g/ ton feedstuff is applied to conventional feed.
The invention has the beneficial effects as follows: the present invention can improve animal body specificity and non-specific immunity, strengthen
Body resistance against diseases, reduces dosage during cultivation, reduces drug residue；Regulation animal gastrointestinal tract colony balance, promotes nutrition
Overall absorption, reduces the aquaculture cost such as feedstuff-meat ratio, feedstuff-egg ratio；Improve breeding environment, reduce the harmful gass such as ammonia, release mycete
Toxin, reduces the harm that feed mold causes；Improve animal product quality, improve culture benefit.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is made further:
Embodiment one
A kind of fermented tcm feed addictive prescription, is made up of by weight following raw materials: Flos Chrysanthemi Indici 10 parts, Fructus Ligustri Lucidi 10
Part, Fructus Crataegi 10 parts, Herba Leonuri 10 parts, Folium Isatidis 10 parts, Caulis Lonicerae 10 parts, Radix Scutellariae 10 parts, liquid spawn 2 parts.
Further, described liquid spawn is made up of by weight following raw materials: Enterococcus faecalis fermentation liquid 1 part, withered
Grass bacillus cereus 0.1 part, Bacillus licheniformis 0.1 part, Clostridium butyricum 0.1 part, saccharomyces cerevisiae 0.1 part, glucose 20 parts, waking up the patient from unconsciousness by clearing away heat
1 part of water.
Wherein, the preparation method of described Enterococcus faecalis fermentation liquid is: (1) inoculates excrement on one-level solid medium 100ml
Enterococcus 1ml(wherein strain concentration is 100,000,000/ml), cultivate 18 hours, obtain primary cultivation strain (described solid for 35 DEG C
Culture medium raw material and preparation method be: takes peptone 0.5% yeast powder 0.3% lactose 1% by weight proportion, and agar powder 1.5% is remaining
Above-mentioned each raw material for water, is made aqueous solution by amount, adjusts PH to 6.5, in subpackage to eggplant-shape bottle, and 115 DEG C of sterilizings 30 minutes, place
Obtain solid medium)；
(2) primary strain of cultivating that step (1) obtains is taken according to strain: fluid medium connects by weight the ratio of 0.5:100
Plant in fluid medium, cultivate 8 hours for 35 DEG C, during wherein fluid medium is placed on fermentation tank, keep tank pressure 0.02MPa,
(described liquid medium starting material and preparation method are to take peptone 1.0%, yeast by weight proportion to obtain secondary cultivation strain
Powder 0.3%, glucose 1.0%；Sodium acetate 0.2%, potassium dihydrogen phosphate 0.2%, ammonium citrate 0.2%, surplus is water, by above-mentioned each former
Aqueous solution made by material, adjusts PH to 6.5,100 DEG C of sterilizings 15 minutes, is cooled to 35 DEG C and i.e. obtains fluid medium)；
(3) the secondary strain of cultivating step (2) obtained cultivates strain according to secondary: fermentation medium is by weight 10:100's
Ratio is inoculated in fermentation medium, cultivates 14 hours for 35 DEG C, during wherein fluid medium is placed on fermentation tank, keeps tank pressure
0.02MPa, (described fermentation culture based raw material and preparation method are to take albumen by weight proportion to obtain Enterococcus faecalis fermentation liquid
Peptone 1.0%, yeast powder 0.3%, glucose 1.0%；Sodium acetate 0.2%, potassium dihydrogen phosphate 0.2%, ammonium citrate 0.2%, calcium carbonate
0.6%, surplus is water, and above-mentioned each raw material makes aqueous solution, adjusts PH to 6.5,100 DEG C of sterilizings 15 minutes, is cooled to 35 DEG C i.e.
Obtain fermentation medium).
Embodiment two
A kind of fermented tcm feed addictive prescription, is made up of by weight following raw materials: Flos Chrysanthemi Indici 20 parts, Fructus Ligustri Lucidi 20
Part, Fructus Crataegi 20 parts, Herba Leonuri 20 parts, Folium Isatidis 20 parts, Caulis Lonicerae 20 parts, Radix Scutellariae 20 parts, liquid spawn 10 parts.
Further, described liquid spawn is made up of by weight following raw materials: Enterococcus faecalis fermentation liquid 1000 parts,
Bacillus subtilis 1 part, Bacillus licheniformis 1 part, Clostridium butyricum 1 part, saccharomyces cerevisiae 1 part, glucose 30 parts, cold water 1000
Part.
Wherein, the preparation method of described Enterococcus faecalis fermentation liquid is: (1) inoculates excrement on one-level solid medium 150ml
Enterococcus 2ml(wherein strain concentration is 1,000,000,000/ml), cultivate 24 hours for 37 DEG C, (described consolidates to obtain primary cultivation strain
Body culture medium raw material and preparation method be: takes peptone 1.5% yeast powder 1.0% lactose 2% by weight proportion, agar powder 2.0%,
Surplus is water, and above-mentioned each raw material makes aqueous solution, adjusts PH to 7.5, in subpackage to eggplant-shape bottle, and 115 DEG C of sterilizings 30 minutes, put
Put and i.e. obtain solid medium)；
(2) primary strain of cultivating that step (1) obtains is taken according to strain: fluid medium connects by weight the ratio of 0.5:100
Plant in fluid medium, cultivate 16 hours for 37 DEG C, during wherein fluid medium is placed on fermentation tank, keep tank pressure
0.04MPa, (described liquid medium starting material and preparation method are to take peptone by weight proportion to obtain secondary cultivation strain
2.5%, yeast powder 1.0%, glucose 3.0%；Sodium acetate 0.7%, potassium dihydrogen phosphate 0.5%, ammonium citrate 0.5%, surplus is water, will
Aqueous solution made by above-mentioned each raw material, adjusts PH to 7.5,121 DEG C of sterilizings 20 minutes, is cooled to 37 DEG C and i.e. obtains fluid medium)；
(3) the secondary strain of cultivating step (2) obtained cultivates strain according to secondary: fermentation medium is by weight 10:100's
Ratio is inoculated in fermentation medium, cultivates 24 hours for 37 DEG C, during wherein fluid medium is placed on fermentation tank, keeps tank pressure
0.04MPa, (described fermentation culture based raw material and preparation method are to take albumen by weight proportion to obtain Enterococcus faecalis fermentation liquid
Peptone 4.0%, yeast powder 1.0%, glucose 5.0%；Sodium acetate 0.7%, potassium dihydrogen phosphate 0.5%, ammonium citrate 0.5%, calcium carbonate
1.6%, surplus is water, and above-mentioned each raw material makes aqueous solution, adjusts PH to 7.5,121 DEG C of sterilizings 20 minutes, is cooled to 37 DEG C
Obtain fermentation medium).
Embodiment three
A kind of fermented tcm feed addictive prescription, is made up of by weight following raw materials: Flos Chrysanthemi Indici 15 parts, Fructus Ligustri Lucidi 15
Part, Fructus Crataegi 15 parts, Herba Leonuri 15 parts, Folium Isatidis 15 parts, Caulis Lonicerae 15 parts, Radix Scutellariae 15 parts, liquid spawn 5 parts.
Further, described liquid spawn is made up of by weight following raw materials: Enterococcus faecalis fermentation liquid 500 parts,
Bacillus subtilis 0.5 part, Bacillus licheniformis 0.5 part, Clostridium butyricum 0.5 part, saccharomyces cerevisiae 0.5 part, glucose 25 parts, cool
500 parts of boiled water.
Wherein, the preparation method of described Enterococcus faecalis fermentation liquid is: (1) inoculates excrement on one-level solid medium 130ml
Enterococcus 1.5ml(wherein strain concentration is 500,000,000/ml), cultivate 21 hours for 36 DEG C, (described consolidates to obtain primary cultivation strain
Body culture medium raw material and preparation method be: takes peptone 1% yeast powder 0.6% lactose 1.5% by weight proportion, agar powder 1.7%,
Surplus is water, and above-mentioned each raw material makes aqueous solution, adjusts PH to 7, in subpackage to eggplant-shape bottle, and 115 DEG C of sterilizings 30 minutes, place
Obtain solid medium)；
(2) primary strain of cultivating that step (1) obtains is taken according to strain: fluid medium connects by weight the ratio of 0.5:100
Plant in fluid medium, cultivate 12 hours for 36 DEG C, during wherein fluid medium is placed on fermentation tank, keep tank pressure 0.03MPa,
(described liquid medium starting material and preparation method are to take peptone 1.8%, yeast by weight proportion to obtain secondary cultivation strain
Powder 0.7%, glucose 2%；Sodium acetate 0.5%, potassium dihydrogen phosphate 0.3%, ammonium citrate 0.4%, surplus is water, by above-mentioned each raw material
Make aqueous solution, adjust PH to 7,110 DEG C of sterilizings 17 minutes, be cooled to 36 DEG C and i.e. obtain fluid medium)；
(3) the secondary strain of cultivating step (2) obtained cultivates strain according to secondary: fermentation medium is by weight 10:100's
Ratio is inoculated in fermentation medium, cultivates 18 hours for 36 DEG C, during wherein fluid medium is placed on fermentation tank, keeps tank pressure
0.03MPa, (described fermentation culture based raw material and preparation method are to take albumen by weight proportion to obtain Enterococcus faecalis fermentation liquid
Peptone 2.5%, yeast powder 0.7%, glucose 3.5%；Sodium acetate 0.5%, potassium dihydrogen phosphate 0.4%, ammonium citrate 0.4%, calcium carbonate
1.1%, surplus is water, and above-mentioned each raw material makes aqueous solution, adjusts PH to 7,110 DEG C of sterilizings 18 minutes, is cooled to 36 DEG C and get final product
Fermentation medium).
It is last it should be noted that detailed description of the invention is to further illustrate the present invention and unrestricted, to this area
For those of ordinary skill, can do in the case of without departing from flesh and blood of the present invention and convert further, and all these change
Change the protection domain that all should belong to claims of the present invention.
Claims (6)
1. a fermented tcm feed addictive prescription, it is characterised in that: it is made up of by weight following raw materials: Flos Chrysanthemi Indici
10-20 part, Fructus Ligustri Lucidi 10-20 part, Fructus Crataegi 10-20 part, Herba Leonuri 10-20 part, Folium Isatidis 10-20 part, Caulis Lonicerae 10-20 part,
Radix Scutellariae 10-20 part, liquid spawn 2-10 part.
A kind of fermented tcm feed addictive prescription the most according to claim 1, it is characterised in that: pressed by following raw materials
Parts by weight form: Flos Chrysanthemi Indici 15 parts, Fructus Ligustri Lucidi 15 parts, Fructus Crataegi 15 parts, Herba Leonuri 15 parts, Folium Isatidis 15 parts, Caulis Lonicerae 15 parts,
Radix Scutellariae 15 parts, liquid spawn 5 parts.
3. according to the arbitrary described a kind of fermented tcm feed addictive prescription of claim 1 or 2, it is characterised in that: described liquid
Body strain is made up of by weight following raw materials: Enterococcus faecalis fermentation liquid 1-1000 part, bacillus subtilis 0.1-1 part,
Bacillus licheniformis 0.1-1 part, Clostridium butyricum 0.1-1 part, saccharomyces cerevisiae 0.1-1 part, glucose 20-30 part, cold water 1-
1000 parts.
A kind of fermented tcm feed addictive prescription the most according to claim 3, it is characterised in that: described enterococcus faecalis is sent out
The preparation method of ferment liquid is:
(1) on one-level solid medium, enterococcus faecalis is inoculated, according to strain: solid medium is 1-2ml:100-by volume
Ratio inoculation (wherein strain concentration is 1-10 hundred million/ml) of 150ml, cultivates 18-24 hour, obtains primary cultivation for 35-37 DEG C
(described solid culture based raw material and preparation method be strain: takes peptone 0.5 1.5% yeast powder 0.3-by weight proportion
1.0% lactose 1-2%, agar powder 1.5-2.0%, surplus is water, and above-mentioned each raw material is made aqueous solution, adjusts PH to 6.5-7.5,
Subpackage in eggplant-shape bottle, 115 DEG C of sterilizings 30 minutes, is placed and is i.e. obtained solid medium)；
(2) primary strain of cultivating that step (1) obtains is taken according to strain: fluid medium connects by weight the ratio of 0.5:100
Plant in fluid medium, cultivate 8-16 hour, during wherein fluid medium is placed on fermentation tank, keep tank pressure for 35-37 DEG C
0.03 ± 0.01MPa, (described liquid medium starting material and preparation method are to take by weight proportion to obtain secondary cultivation strain
Peptone 1.0-2.5%, yeast powder 0.3-1.0%, glucose 1.0-3.0%；Sodium acetate 0.2-0.7%, potassium dihydrogen phosphate 0.2-
0.5%, ammonium citrate 0.2-0.5%, surplus is water, and above-mentioned each raw material is made aqueous solution, adjusts PH to 6.5-7.5,100-121
DEG C sterilizing 15-20 minute, is cooled to 35-37 DEG C and i.e. obtains fluid medium)；
(3) the secondary strain of cultivating step (2) obtained cultivates strain according to secondary: fermentation medium is by weight 10:100's
Ratio is inoculated in fermentation medium, cultivates 14-24 hour for 35-37 DEG C, during wherein fluid medium is placed on fermentation tank, keeps tank
Pressure 0.03 ± 0.01MPa, (described fermentation culture based raw material and preparation method are, by weight to obtain Enterococcus faecalis fermentation liquid
Example takes peptone 1.0-4.0%, yeast powder 0.3-1.0%, glucose 1.0-5.0%；Sodium acetate 0.2-0.7%, potassium dihydrogen phosphate
0.2-0.5%, ammonium citrate 0.2-0.5%, calcium carbonate 0.6-1.6%, surplus is water, and above-mentioned each raw material is made aqueous solution, adjusts
PH to 6.5-7.5,100-121 DEG C of sterilizing 15-20 minute, is cooled to 35-37 DEG C and i.e. obtains fermentation medium).
5., according to the production technology of the arbitrary described a kind of fermented tcm feed addictive prescription of claim 1 or 2, its feature exists
In: described production technology is, takes the Flos Chrysanthemi Indici of corresponding weight, Fructus Ligustri Lucidi, Fructus Crataegi, Herba Leonuri, Folium Isatidis according to above-mentioned formula, bears
Caulis Lonicerae, Radix Scutellariae, insert each component in grinder-mixer, is pulverized and mixed uniformly, adds the liquid spawn of corresponding weight, and mixing is all
Even, in subpackage to the one-way breather valve fermentation bag sealed, 30-37 DEG C ferments 48-120 hour, gets product.
6. according to the arbitrary described a kind of fermented tcm feed addictive prescription of claim 1 or 2, it is characterised in that: described
Ferment Chinese medicine feed additive is to be applied to routine according to the ratio of 1000-3000g/ ton feedstuff raise at the application process of field of fodder
Material.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106923018A (en) * | 2017-03-24 | 2017-07-07 | 合肥慧谷农业科技有限公司 | A kind of fermented pig feed addictive and preparation method thereof |
| CN108094681A (en) * | 2018-01-04 | 2018-06-01 | 商丘塞弗顿生物工程有限公司 | A kind of de- mould feed addictive and preparation method thereof that detoxifies |
| CN108935962A (en) * | 2018-09-30 | 2018-12-07 | 福建贝迪药业有限公司 | A kind of pulvis and preparation method thereof taking off mould protect liver for livestock and poultry |
| CN110419643A (en) * | 2019-09-04 | 2019-11-08 | 刘泳君 | Fermented tcm preparation and the preparation method and application thereof |
| CN113832059A (en) * | 2021-09-23 | 2021-12-24 | 洛阳欧科拜克生物技术股份有限公司 | Antibacterial bacillus and solid fermentation method thereof |
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| CN101275158A (en) * | 2007-11-05 | 2008-10-01 | 孙卫 | Screening preparation for superior enterococcus faecalis and application thereof |
| CN105724772A (en) * | 2016-02-24 | 2016-07-06 | 济南亿民动物药业有限公司 | Chinese herbal medicine micro-ecological compound feed additive and preparation and use method thereof |
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| CN101275158A (en) * | 2007-11-05 | 2008-10-01 | 孙卫 | Screening preparation for superior enterococcus faecalis and application thereof |
| CN101220342A (en) * | 2007-11-14 | 2008-07-16 | 大连三仪动物药品有限公司 | FQ15 enterococcus faecalis and method for producing somatotrophic feed additive with the bacteria |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106923018A (en) * | 2017-03-24 | 2017-07-07 | 合肥慧谷农业科技有限公司 | A kind of fermented pig feed addictive and preparation method thereof |
| CN108094681A (en) * | 2018-01-04 | 2018-06-01 | 商丘塞弗顿生物工程有限公司 | A kind of de- mould feed addictive and preparation method thereof that detoxifies |
| CN108935962A (en) * | 2018-09-30 | 2018-12-07 | 福建贝迪药业有限公司 | A kind of pulvis and preparation method thereof taking off mould protect liver for livestock and poultry |
| CN110419643A (en) * | 2019-09-04 | 2019-11-08 | 刘泳君 | Fermented tcm preparation and the preparation method and application thereof |
| CN110419643B (en) * | 2019-09-04 | 2023-05-23 | 刘泳君 | Fermented traditional Chinese medicine preparation and preparation method and application thereof |
| CN113832059A (en) * | 2021-09-23 | 2021-12-24 | 洛阳欧科拜克生物技术股份有限公司 | Antibacterial bacillus and solid fermentation method thereof |
| CN113832059B (en) * | 2021-09-23 | 2024-02-09 | 洛阳欧科拜克生物技术股份有限公司 | Antibacterial bacillus and solid fermentation method thereof |
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Application publication date: 20170111 |