CN107619314A - A kind of chicken manure quick composting technique - Google Patents
A kind of chicken manure quick composting technique Download PDFInfo
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- CN107619314A CN107619314A CN201710194857.5A CN201710194857A CN107619314A CN 107619314 A CN107619314 A CN 107619314A CN 201710194857 A CN201710194857 A CN 201710194857A CN 107619314 A CN107619314 A CN 107619314A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The present invention is directed to a large amount of chicken manures of output in large-scale poultry place and accumulated for a long time, decomposed period length, the problems such as decomposed effect difference, there is provided a kind of new microorganism rapid decomposition method.The present invention uses the fresh chicken manure of chicken farm output, mixes the chicken house bedding and padding such as rice husk, peanut shell, stalk according to a certain percentage, adjusts water content and pH etc., carries out the fermentation of aerobic and anaerobism two benches.Corresponding strain is inoculated with fermentation process respectively and is carried out according to certain turning technique.Technique used in the present invention and method can complete the decomposed of chicken manure at 20 days or so, while removed the stink being pungent in chicken manure and ammonia taste, kill roundworm egg and pathogenic microorganism therein, obtain the chicken manure decomposing agent with light Earthy Taste.Probiotics rich content therein, nutritional ingredient are balanced, have obvious preventive and therapeutic effect to pest and disease damage in soil.
Description
Technical field
The present invention relates to a kind of using the fermentation of microbial bacterial agent progress segmented, the technology of quick composting chicken manure, belong to foster
Grow offal treatment and bio-feritlizer manufacturing technology field.
Background technology
With the fast development of aquaculture, the yield of feces of livestock and poultry is huge, most due to lacking effective processing skill
The reasons such as art fail to make full use of, and a large amount of prolonged accumulations are discarded to cause serious pollution to environment, endangers the body of people
Body health.
Fowl and animal excrement contains the nutriments such as substantial amounts of protein, if can be subject to scientific utilization can just be turned waste into wealth.
Therefore, it is to solve problem of environmental pollution harmless treatment and application to be carried out to fowl and animal excrement with the method for science, realizes cultivation
Industry sustainable development, the unique channel for increasing culture benefit.
The content of the invention
The present invention is in view of the above-mentioned problems, disclose a kind of quick based on the interim inoculation of microbial bacterial agent, the chicken manure to ferment
Decomposed technique.
1st, prepared by shake-flask seed
It is prepared by bacillus shake-flask seed：
Bacillus is inoculated into liquid shaking bottle, liquid amount is to fill liquid 80-220ml per 1L shaking flasks, and Medium Proportion is：Dregs of beans
1.00%-5.00%, corn flour 0.80%-3.50%, glucose 0.30%-1.00%, manganese sulfate 0.01%-0.05%, pH is adjusted before sterilizing
To 7.5-9.5, shaking table culture condition：35 DEG C -40 DEG C of temperature, rotating speed 150-250rpm.Incubation time 8-20 hours, obtain viable bacteria
Content reaches 2 × 10^8-10 × 10^8cfu/ml shake-flask seed.
It is prepared by streptomyces microflavus shake-flask seed：
Streptomyces microflavus is inoculated into liquid shaking bottle, liquid amount is to fill liquid 80-220ml per 1L shaking flasks, and Medium Proportion is：Greatly
Bean powder 0.1%-0.9%, glucose 2.0%-2.5%, ammonium sulfate 1.0%-1.9%, sodium chloride 0.2%-0.8%, pH 6.0-8.0, shake
Bed condition of culture：26 DEG C -32 DEG C of cultivation temperature, rotating speed 150-200 rpm.Incubation time 65-80 hours, obtain viable bacteria content and reach
To 5 × 10^8-15 × 10^8cfu/ml shake-flask seed.
It is prepared by Trichoderma harzianum shake-flask seed liquid：
Trichoderma harzianum is inoculated into liquid shaking bottle, liquid amount is to fill liquid 80-220ml per 1L shaking flasks, uses liquid PDA culture medium
Culture.Shaking table culture condition：26 DEG C -32 DEG C of temperature, rotating speed 150-250rpm.Incubation time 48-96 hours, obtain spore content
Reach 5 × 10^8-15 × 10^8cfu/ml shake-flask seed.
It is prepared by lactic acid bacteria shake-flask seed：
A kind of in Bifidobacterium, streptococcus lactis, Lactococcus lactis is inoculated into the examination equipped with 5-10mlMRS fluid nutrient mediums
Guan Zhong, 37 DEG C of quiescent cultures 24 hours, the primary seed solution in test tube is inoculated into according to 1%-5% inoculum concentration and trained equipped with MRS
In the blue mouth bottle for supporting base, 37 DEG C of quiescent cultures 24 hours.Obtain the seed that viable bacteria amount reaches 1 × 10^10-310^10cfu/ml
Liquid.Wherein, the proportioning of MRS culture mediums is：Peptone 0.6%-1.5%, beef extract 0.6%-1.5%, yeast extract 0.2%-0.7%, Portugal
Grape sugar 1.5%-2.5%, Tween 80 0.05%-0.15%, sodium acetate 0.25%-0.6%, magnesium sulfate 0.01%-0.032%, manganese sulfate：
0.002%-0.007%, dipotassium hydrogen phosphate 0.12%-0.27%, natural pH.
It is prepared by saccharomycete shake-flask seed：
Brewer's yeast or Saccharomyces cerevisiae are inoculated into liquid shaking bottle, liquid amount is to fill liquid 80-220ml per 1L shaking flasks, uses liquid
Body YPD medium cultures.Shaking table culture condition：26 DEG C -32 DEG C of temperature, rotating speed 150-250rpm.Incubation time 48-96 hours,
Obtain the shake-flask seed that amount of yeast reaches 1 × 10^8-15 × 2.5^8cfu/ml.
It is prepared by nitrobacteria shake-flask seed：
Hydrogenlike silicon ion or Rhodospirillum rubrum are inoculated into liquid shaking bottle, liquid amount is to fill liquid 100-250ml per 1L shaking flasks, is shaken
Bed condition of culture：25 DEG C -32 DEG C of temperature, rotating speed 80-250rpm.Incubation time 4-5 days.Obtain OD520It is worth for 0.5-0.8 concentration
Seed liquor.Its Medium Proportion is：Potassium nitrite 0.15%-0.2%, epsom salt 0.08%-0.11%, ferrous sulfate heptahydrate
0.1%-0.15%, sodium acid carbonate 0.16%-0.22%, three water dipotassium hydrogen phosphate 0.11%-0.14%, sodium chloride 0.15%-0.22%, carbon
Sour calcium 0.4%-0.55%, ammonium sulfate 0.15%-0.23%, zinc sulfate 0.00011%-0.00013%, cupric sulfate pentahydrate 0.00007%-
0.00009%, pH7.5-8.5.
2nd, the compounding and preparation of decomposing microbial inoculum
By a kind of 1-4 parts in Bifidobacterium seed liquid, streptococcus lactis seed liquor, Lactococcus lactis seed liquor, brewer's yeast
Bacterium seed liquor 3-4.5 parts, bread microzyme seed liquor 3-4.5 parts fully mix, and are configured to anaerobism decomposing microbial inoculum.
By bacillus subtilis seed liquor, bacillus licheniformis seed liquor, bacillus megaterium seed liquor, natto gemma bar
A kind of 3-5 parts in bacterium seed liquor, bacillus amyloliquefaciens seed liquor, hydrogenlike silicon ion seed liquor or Rhodospirillum rubrum seed liquor
0.5-1 parts, Trichoderma harzianum 2-3 parts, streptomyces microflavus seed liquor 2-4 parts fully mix, and are configured to aerobic decomposing microbial inoculum.
3rd, the compounding of first time raw material and pH regulations
Take chicken manure 2-4 parts, a kind of 6-8 parts in rice husk, peanut shell, stalk, put into batch mixer, and according to total material
1.0%-3.5% amount adds one kind or several in the nutriments such as waste saccharide liquid, aminoacids solution, starch wastewater, manioc waste, mushroom residue
Kind, mixing is sufficiently stirred, its pH to 4.0-7.0 is adjusted with phosphoric acid, hydrochloric acid, acetic acid, sulfuric acid etc..
4th, inoculation and anaerobism are decomposed for the first time
According to mixed anaerobism decomposing microbial inoculum is matched somebody with somebody before 1.0%-5.0% inoculum concentration sprinkling access, running water is filled into decomposed thing
Water content reaches 45%-60% in material, is placed in after fully mixing in heat preservation tank and compresses sealed fermenting.Whole anaerobism decomposed period about 10 days
Left and right, temperature gradually rises during this, up to about 60-75 DEG C, and temperature keeps constant or even slow decline afterwards.
5th, second of raw material compounding
Corn steep liquor, glucose, corn are added in the material that addition according to 0.5%-2.0% obtains into anaerobism digest process
One or more in the nutriments such as powder, sucrose, dregs of beans, maltodextrin, fully mix, adjust material pH to 5.0-7.0.
6th, secondary inoculation and aerobic decomposed
According to mixed aerobic fermentation microbial inoculum is matched somebody with somebody before 1.0%-5.0% inoculum concentration sprinkling access, by material code after fully mixing
Into bar pile, temperature rises to 60 DEG C of turnings and is cooled to less than 58 DEG C in heap.This supports well digest process and lasts about 10 days or so, in temperature
Early stage, fluctuation-type rose, and later stage fluctuation-type declines, and normal temperature is down at the end of decomposed；On smell early stage have strongly pungent ammonia taste and
Larger stink.
With decomposed progress, ammonia taste and stink gradually mitigate, and to decomposed end, ammonia taste and stink substantially eliminate, material
With light soil breath.
Advantages of the present invention：The present invention carries out anaerobism and aerobic two stage decomposed by being inoculated with different strain, develops
A kind of complete chicken manure quick composting handling process, solve poultry enterprise chicken manure bulk deposition occupied ground, pollution environment etc.
Problem, while obtain a kind of prebiotic bacterial content height, odorless, tasteless, the high added value organic fertilizer that can directly apply.
Embodiment
The present invention is further described with reference to specific embodiment, so that those skilled in the art knows more about
The present invention, but and it is not so limited the present invention.
Embodiment 1
1st, prepared by shake-flask seed
It is prepared by bacillus shake-flask seed：
Bacillus subtilis is inoculated into liquid shaking bottle, liquid amount is to fill liquid 150ml per 1L shaking flasks, and Medium Proportion is：Beans
The dregs of rice 3. 0%, corn flour 1.50%, glucose 0.3%, manganese sulfate 0.01%, pH is adjusted to 8.0 before sterilizing, shaking table culture condition：Temperature
35 DEG C, rotating speed 150rpm.Incubation time 10 hours, obtain the shake-flask seed that viable bacteria content reaches 2 × 10^8cfu/ml.
It is prepared by streptomyces microflavus shake-flask seed：
Streptomyces microflavus is inoculated into liquid shaking bottle, liquid amount is to fill liquid 150ml per 1L shaking flasks, and Medium Proportion is：Soybean
Powder 0.2%, glucose 2.0%, ammonium sulfate 1.0%, sodium chloride 0.2%, pH 7.0, shaking table culture condition：28 DEG C of cultivation temperature, turn
Fast 150rpm.Incubation time 65 hours, obtain the shake-flask seed that viable bacteria content reaches 5 × 10^8cfu/ml.
It is prepared by Trichoderma harzianum shake-flask seed liquid：
Trichoderma harzianum is inoculated into liquid shaking bottle, liquid amount is to fill liquid 150ml per 1L shaking flasks, is trained using liquid PDA culture medium
Support.Shaking table culture condition：28 DEG C of temperature, rotating speed 150rpm.Incubation time 96 hours, obtains spore content and reaches 5 × 10^
8cfu/ml shake-flask seed.
It is prepared by lactic acid bacteria shake-flask seed：
Bifidobacterium single bacterium colony is inoculated into the test tube equipped with 5mlMRS fluid nutrient mediums, 37 DEG C of quiescent cultures 24 hours, pressed
The primary seed solution in test tube is inoculated into the blue mouth bottle equipped with MRS culture mediums according to 1%-5% inoculum concentration, 37 DEG C stand training
Support 24 hours.Obtain the seed liquor that viable bacteria amount reaches 1 × 10^10cfu/ml.Wherein, the proportioning of MRS culture mediums is：Peptone
0.6%, beef extract 0.6%, yeast extract 0.2%, glucose 1.5%, Tween 80 0.05%, sodium acetate 0.25%, magnesium sulfate 0.01%, sulphur
Sour manganese：0.002%, dipotassium hydrogen phosphate 0.12%, natural pH.
It is prepared by saccharomycete shake-flask seed：
Saccharomyces cerevisiae/brewer's yeast is inoculated into liquid shaking bottle, liquid amount is to fill liquid 150ml per 1L shaking flasks, uses liquid YPD
Medium culture.Shaking table culture condition：28 DEG C of temperature, rotating speed 150rpm.Incubation time 48 hours, obtains amount of yeast and reaches 1
× 10^8cfu/ml shake-flask seed.
It is prepared by nitrobacteria shake-flask seed：
Hydrogenlike silicon ion, Rhodospirillum rubrum are inoculated into liquid shaking bottle respectively, liquid amount is to fill liquid 130ml per 1L shaking flasks, is shaken
Bed condition of culture：29 DEG C of temperature, rotating speed 170rpm.Incubation time 5 days.Obtain OD520It is worth for the seed liquor of 0.6 concentration.It is cultivated
Basigamy ratio is：Potassium nitrite 0.2%, epsom salt 0.08%, ferrous sulfate heptahydrate 0.1%, sodium acid carbonate 0.16%, three water phosphoric acid
Hydrogen dipotassium 0.11%, sodium chloride 0.15%, calcium carbonate 0.4%, ammonium sulfate 0.15%, zinc sulfate 0.00011%, cupric sulfate pentahydrate
0.00007%, pH7.5.
2nd, the compounding and preparation of decomposing microbial inoculum
By 4 parts of Bifidobacterium seed liquid, 3 parts of brewer's yeast seed liquor, 3 parts of Saccharomyces cerevisiae seed liquor fully mixes, and is configured to detest
Oxygen decomposing microbial inoculum.
By 5 parts of bacillus subtilis seed liquor, 0.5 part of Rhodospirillum rubrum seed liquor, 2 parts of Trichoderma harzianum, streptomyces microflavus
2.5 parts of seed liquor fully mixes, and is configured to aerobic decomposing microbial inoculum.
3rd, the compounding of first time raw material and pH regulations
2 parts of chicken manure is taken, 8 parts of rice husk, is put into batch mixer, and battalion is used as according to the 1. 5% amount addition aminoacids solution of total material
Material is supported, mixing is sufficiently stirred, its pH to 7.0 is adjusted with phosphoric acid.
4th, inoculation and anaerobism are decomposed for the first time
According to mixed anaerobism decomposing microbial inoculum is matched somebody with somebody before 1.8% inoculum concentration sprinkling access, fill into running water and contain into decomposed material
Water reaches 45%-60%, is placed in after fully mixing in heat preservation tank and compresses sealed fermenting.Whole anaerobism decomposed period totally 8 days, this process
Middle temperature gradually rises, up to 70 DEG C, and temperature keeps constant or even slow decline afterwards.
5th, second of raw material compounding
Corn steep liquor is added in the material obtained according to 1.5% addition into anaerobism digest process, is fully mixed, adjusts material
PH to 7.0.
6th, secondary inoculation and aerobic decomposed
According to mixed aerobic fermentation microbial inoculum match somebody with somebody before 1.9% inoculum concentration sprinkling access, by material code into bar fully after mixing
Pile, temperature rises to 60 DEG C of turnings and is cooled to less than 58 DEG C in heap.This supports well digest process and lasts about 9 days, fluctuation early stage in temperature
Formula rises, and later stage fluctuation-type declines, and temperature is down to 30 DEG C at the 10th day, and no longer heats up.
At the end of decomposed, material ammonia taste, stink substantially eliminate, and have light soil breath.
Embodiment 2
1st, prepared by shake-flask seed
It is prepared by bacillus shake-flask seed：
Bacillus amyloliquefaciens are inoculated into liquid shaking bottle, liquid amount is to fill liquid 140ml per 1L shaking flasks, and Medium Proportion is：
Dregs of beans 2.0%%, corn flour 1.2%, glucose 0.60%, manganese sulfate 0.03%, pH is adjusted to 8.5 before sterilizing, shaking table culture condition：Temperature
34 DEG C of degree, rotating speed 170rpm.Incubation time 15 hours, obtain the shake-flask seed that viable bacteria content reaches 4.5 × 10^8cfu/ml.
It is prepared by streptomyces microflavus shake-flask seed：
Streptomyces microflavus is inoculated into liquid shaking bottle, liquid amount is to fill liquid 140ml per 1L shaking flasks, and Medium Proportion is：Soybean
Powder 0.7%, glucose 21%, ammonium sulfate 1.4%, sodium chloride 0.6%, pH 8.0, shaking table culture condition：28 DEG C of cultivation temperature, turn
180 rpm of speed.Incubation time 76 hours, obtain the shake-flask seed that viable bacteria content reaches 8 × 10^8cfu/ml.
It is prepared by Trichoderma harzianum shake-flask seed liquid：
Trichoderma harzianum is inoculated into liquid shaking bottle, liquid amount is to fill liquid 1200ml per 1L shaking flasks, is trained using liquid PDA culture medium
Support.Shaking table culture condition：26 DEG C of temperature, rotating speed 150rpm.Incubation time 72 hours, obtains spore content and reaches 7 × 10^
8cfu/ml shake-flask seed.
It is prepared by lactic acid bacteria shake-flask seed：
Streptococcus lactis single bacterium colony is inoculated into the test tube equipped with 10mlMRS fluid nutrient mediums, 24 hours 37 DEG C of quiescent cultures,
It is inoculated into according to 2% inoculum concentration in the blue mouth bottle equipped with MRS culture mediums, 24 hours 37 DEG C of quiescent cultures.Viable bacteria amount is obtained to reach
1.5 × 10^10cfu/ml seed liquor.Wherein, the proportioning of MRS culture mediums is：Peptone 0.8%, beef extract 0.8%, yeast extract
0.5%, glucose 1.8%, Tween 80 0.06%, sodium acetate 0.28%, magnesium sulfate 0.02%, manganese sulfate：0.003%, dipotassium hydrogen phosphate
0.18%, natural pH.
It is prepared by saccharomycete shake-flask seed：
Brewer's yeast, Saccharomyces cerevisiae are inoculated into liquid shaking bottle respectively, liquid amount is to fill liquid 130ml per 1L shaking flasks, uses liquid
Body YPD medium cultures.Shaking table culture condition：28 DEG C of temperature, rotating speed 210rpm.Incubation time 68 hours, obtains amount of yeast
Reach 1.6 × 10^8cfu/ml shake-flask seed.
It is prepared by nitrobacteria shake-flask seed：
Rhodospirillum rubrum is inoculated into liquid shaking bottle, liquid amount is to fill liquid 250ml, shaking table culture condition per 1L shaking flasks：Temperature
32 DEG C, rotating speed 250rpm.Incubation time 4 days.Obtain OD520It is worth for the seed liquor of 0.8 concentration.Its Medium Proportion is：Nitrous acid
Potassium 0.2%, epsom salt 0.11%, ferrous sulfate heptahydrate 0.1%, sodium acid carbonate 0.16%, three water dipotassium hydrogen phosphates 0.14%, chlorine
Change sodium 0.22%, calcium carbonate 0.4%, ammonium sulfate 0.18%, zinc sulfate 0.00012%, cupric sulfate pentahydrate 0.00009%, pH7.9.
2nd, the compounding and preparation of decomposing microbial inoculum
By 3 parts of streptococcus lactis seed liquor, 3 parts of brewer's yeast seed liquor, 4 parts of Saccharomyces cerevisiae seed liquor fully mixes, and is configured to
Anaerobism decomposing microbial inoculum.
By 3 parts of bacillus amyloliquefaciens seed liquor, 1 part of Rhodospirillum rubrum seed liquor, 3 parts of Trichoderma harzianum, streptomyces microflavus
3 parts of seed liquor fully mixes, and is configured to aerobic decomposing microbial inoculum.
3rd, the compounding of first time raw material and pH regulations
4 parts of chicken manure is taken, 6 parts of stalk, is put into batch mixer, and waste saccharide liquid is added as nutrients according to 3.5% amount of total material
Matter, mixing is sufficiently stirred, its pH to 5.5 is adjusted with hydrochloric acid.
4th, inoculation and anaerobism are decomposed for the first time
According to mixed anaerobism decomposing microbial inoculum is matched somebody with somebody before 2.0% inoculum concentration sprinkling access, fill into running water and contain into decomposed material
Water is placed in incubation chamber after fully mixing up to 60% and compresses sealed fermenting.Whole anaerobism decomposed period is 12 days, during this
Temperature gradually rises, and temperature rises to highest, about 68 DEG C at the 9th day, is gradually reduced afterwards, 59 DEG C were down to the 12nd day.
5th, second of raw material compounding
Corn steep liquor is added in the material obtained according to 2.5% addition into anaerobism digest process as quick-acting carbon sources, it is fully mixed
It is even, adjust material pH to 6.6.
6th, secondary inoculation and aerobic decomposed
According to mixed aerobic fermentation microbial inoculum match somebody with somebody before 2.0% inoculum concentration sprinkling access, by material code into bar fully after mixing
Pile, temperature rises to 60 DEG C of turnings and is cooled to less than 58 DEG C in heap.This supports well digest process and lasts about 11 days, fluctuation early stage in temperature
Formula rises, and later stage fluctuation-type declines, and no longer rises to temperature at the 11st day, is maintained at 27 DEG C.
At the end of decomposed, ammonia taste and stink disappear substantially, and material carries light soil breath.
Embodiment 3
1st, prepared by shake-flask seed
It is prepared by bacillus shake-flask seed：
Bafillus natto is inoculated into liquid shaking bottle, liquid amount is to fill liquid 180ml per 1L shaking flasks, and Medium Proportion is：Beans
The dregs of rice 4.0%, corn flour 2.50%, glucose 0.5%, manganese sulfate 0.4%, pH is adjusted to 9.5 before sterilizing, shaking table culture condition：Temperature 40
DEG C, rotating speed 250rpm.Incubation time 20 hours, obtain the shake-flask seed that viable bacteria content reaches 7 × 10^8cfu/ml.
It is prepared by streptomyces microflavus shake-flask seed：
Streptomyces microflavus is inoculated into liquid shaking bottle, liquid amount is to fill liquid 160ml per 1L shaking flasks, and Medium Proportion is：Soybean
Powder 0.3%, glucose 2.0%, ammonium sulfate 1.7%, sodium chloride 0.7%, pH 8.0, shaking table culture condition：32 DEG C of cultivation temperature, turn
200 rpm of speed.Incubation time 80 hours, obtain the shake-flask seed that viable bacteria content reaches 12 × 10^8cfu/ml.
It is prepared by Trichoderma viride shake-flask seed liquid：
Trichoderma viride is inoculated into liquid shaking bottle, liquid amount is to fill liquid 130ml per 1L shaking flasks, is trained using liquid PDA culture medium
Support.Shaking table culture condition：32 DEG C of temperature, rotating speed 250rpm.Incubation time 96 hours, obtains spore content and reaches 13 × 10^
8cfu/ml shake-flask seed.
It is prepared by lactic acid bacteria shake-flask seed：
Picking Lactococcus lactis single bacterium colony is inoculated into the test tube equipped with 8mlMRS fluid nutrient mediums, 37 DEG C of standing trainings in 24 hours
Support, be inoculated into according to 4% inoculum concentration in the blue mouth bottle equipped with MRS culture mediums, 24 hours 37 DEG C of quiescent cultures.Obtain viable bacteria amount
Reach 2 × 10^10cfu/ml seed liquor.Wherein, the proportioning of MRS culture mediums is：Peptone 1.5%, beef extract 0.9%, yeast
Cream 0.5%, glucose 1.9%, Tween 80 0.13%, sodium acetate 0.34%, magnesium sulfate 0.02%, manganese sulfate：0.005%, phosphoric acid hydrogen two
Potassium 0.22%, natural pH.
It is prepared by saccharomycete shake-flask seed：
Brewer's yeast, Saccharomyces cerevisiae are inoculated into liquid shaking bottle respectively, liquid amount is to fill liquid 130ml per 1L shaking flasks, uses liquid
Body YPD medium cultures.Shaking table culture condition：32 DEG C of temperature, rotating speed 250rpm.Incubation time 96 hours, obtains amount of yeast
Reach 1.4 × 10^8cfu/ml shake-flask seed.
It is prepared by nitrobacteria shake-flask seed：
Hydrogenlike silicon ion is inoculated into liquid shaking bottle, liquid amount is to fill liquid 170ml, shaking table culture condition per 1L shaking flasks：Temperature
30 DEG C, rotating speed 130rpm.Incubation time 5 days.Obtain OD520It is worth for the seed liquor of 0.75 concentration.Its Medium Proportion is：Nitrous
Sour potassium 0.19, epsom salt 0.09%, ferrous sulfate heptahydrate 0.13%, sodium acid carbonate 0.18%, three water dipotassium hydrogen phosphates 0.12%,
Sodium chloride 0.16%, calcium carbonate 0.48%, ammonium sulfate 0.20%, zinc sulfate 0.00012%, cupric sulfate pentahydrate 0.00008%, pH8.0.
2nd, the compounding and preparation of decomposing microbial inoculum
2 parts of Lactococcus lactis seed liquor, 4.5 parts of saccharomyces cerevisiae seed liquor, 3.5 parts of bread microzyme seed liquor is fully mixed
It is even, it is configured to anaerobism decomposing microbial inoculum.
By 4 parts of bafillus natto seed liquor, 0.8 part of hydrogenlike silicon ion seed liquor, 2.7 parts of Trichoderma harzianum, thin yellow strepto-
2.5 parts of bacterium seed liquor fully mixes, and is configured to aerobic decomposing microbial inoculum.
3rd, the compounding of first time raw material and pH regulations
3 parts of chicken manure is taken, 7 parts of peanut shell, is put into batch mixer, and mushroom residue is added as nutrition according to 2.5% amount of total material
Material, mixing is sufficiently stirred, its pH to 6.5 is adjusted with sulfuric acid etc..
4th, inoculation and anaerobism are decomposed for the first time
According to mixed anaerobism decomposing microbial inoculum is matched somebody with somebody before 5.0% inoculum concentration sprinkling access, fill into running water and contain into decomposed material
Water is placed in incubation chamber after fully mixing up to 55% and compresses sealed fermenting.Entirely anaerobism is decomposed lasts about 8 days, this process medium temperature
Degree gradually rises, and 65 DEG C of maximum temperature is risen at the 7th day, temperature is down to 62 DEG C within the 8th day.
5th, second of raw material compounding
Corn flour is added in the material obtained according to 2.5% addition into anaerobism digest process as quick-acting carbon sources, it is fully mixed
It is even, adjust material pH to 6.8 or so.
6th, secondary inoculation and aerobic decomposed
According to mixed aerobic fermentation microbial inoculum match somebody with somebody before 3.0% inoculum concentration sprinkling access, by material code into bar fully after mixing
Pile, temperature rises to 60 DEG C of turnings and is cooled to less than 58 DEG C in heap.This supports well digest process and lasts about 10 days or so, early stage in temperature
Fluctuation-type rises, and later stage fluctuation-type declines, and no longer rises to the tenth day temperature, stable at 28 DEG C.
At the end of decomposed, ammonia taste and stink almost disappear, and have light soil delicate fragrance.
Claims (9)
1. a kind of chicken manure quick composting technique, it is characterised in that the decomposing microbial inoculum includes anaerobism decomposing microbial inoculum and aerobic decomposed
Microbial inoculum.
2. as claimed in claim 1, anaerobism decomposing microbial inoculum includes Bifidobacterium, streptococcus lactis, Lactococcus lactis, brewer's yeast
Bacterium, bread microzyme.
3. as claimed in claim 1, aerobic decomposing microbial inoculum include bacillus subtilis, bacillus licheniformis, bacillus megaterium,
Bafillus natto, bacillus amyloliquefaciens, hydrogenlike silicon ion, Rhodospirillum rubrum, Trichoderma harzianum, streptomyces microflavus.
4. as claimed in claim 1, zymotechnique comprises the following steps：
Shake-flask seed preparation, the compounding of decomposing microbial inoculum and preparation, the compounding of first time raw material and pH regulations, for the first time inoculation and anaerobism
Decomposed, second raw material compounding and pH regulations, second of inoculation and aerobic decomposed.
5. as claimed in claim 1, the compounding of decomposing microbial inoculum and preparation method are as follows：
Take a kind of 1-4 parts in Bifidobacterium seed liquid, streptococcus lactis seed liquor, Lactococcus lactis seed liquor, brewer's yeast
Bacterium seed liquor 3-4.5 parts, bread microzyme seed liquor 3-4.5 parts fully mix, and are configured to anaerobism decomposing microbial inoculum.
Take bacillus subtilis seed liquor, bacillus licheniformis seed liquor, bacillus megaterium seed liquor, bafillus natto kind
A kind of 3-5 parts in sub- liquid, bacillus amyloliquefaciens seed liquor, in hydrogenlike silicon ion seed liquor, Rhodospirillum rubrum seed liquor
A kind of 0.5-1 parts, Trichoderma harzianum 2-3 parts, streptomyces microflavus seed liquor 2-4 parts fully mix, and are configured to aerobic decomposing microbial inoculum.
6. as claimed in claim 1, the compounding of first time raw material and pH adjusting methods are as follows：
Take chicken manure 2-4 parts, a kind of 6-8 parts in rice husk, peanut shell, stalk, put into batch mixer, and according to total material
1.0%-3.5% amount adds the one kind of nutriment or several such as waste saccharide liquid, aminoacids solution, starch wastewater, manioc waste, mushroom residue
Kind, mixing is sufficiently stirred, its pH to 4.0-7.0 is adjusted with phosphoric acid, hydrochloric acid, acetic acid, sulfuric acid etc..
7. as claimed in claim 1, inoculation and the decomposed method of anaerobism are as follows for the first time：
According to mixed anaerobism decomposing microbial inoculum is matched somebody with somebody before 1.0%-5.0% inoculum concentration sprinkling access, running water is filled into decomposed thing
Water content reaches 45%-60% in material, is placed in after fully mixing in heat preservation tank and compresses sealed fermenting.
8. as claimed in claim 1, second of raw material compounding and pH adjusting methods are as follows：
Corn steep liquor, glucose, corn are added in the material that addition according to 0.5%-2.0% obtains into anaerobism digest process
One or more in the nutriments such as powder, sucrose, dregs of beans maltodextrin, fully mix, adjust material pH to 5.0-7.0.
9. as claimed in claim 1, second of inoculation and aerobic decomposed method are as follows：
According to mixed aerobic fermentation microbial inoculum is matched somebody with somebody before 1.0%-5.0% inoculum concentration sprinkling access, by material code after fully mixing
Into bar pile, temperature rises to 60 DEG C of turnings and is cooled to less than 58 DEG C in heap.
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| CN109370885A (en) * | 2018-12-14 | 2019-02-22 | 莱西市产业技术研究院 | A kind of chicken farm anaerobic fermentation system and its application method |
| CN109369228A (en) * | 2018-11-20 | 2019-02-22 | 自贡禾稷旭茂农业开发有限公司 | A kind of method that can promote the decomposed speed of solid organic fertilizer |
| CN112279695A (en) * | 2020-10-22 | 2021-01-29 | 广西晋有有机肥有限公司 | Biological organic fertilizer |
| CN115746857A (en) * | 2022-11-08 | 2023-03-07 | 甘肃省农业科学院旱地农业研究所 | Preparation method of soil conditioner |
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