CN108617861A - A kind of perfect compound feed meeting finishing period alpaca lysine demand - Google Patents

A kind of perfect compound feed meeting finishing period alpaca lysine demand Download PDF

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CN108617861A
CN108617861A CN201810443427.7A CN201810443427A CN108617861A CN 108617861 A CN108617861 A CN 108617861A CN 201810443427 A CN201810443427 A CN 201810443427A CN 108617861 A CN108617861 A CN 108617861A
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feed
alpaca
lysine
group
diet
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王述柏
谭子超
郝薪云
刘晓龙
于超
姜建阳
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Qingdao Agricultural University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/10Feeding-stuffs specially adapted for particular animals for ruminants
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A23K10/38Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/26Compounds containing phosphorus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/30Oligoelements
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The present invention provides a kind of feed meeting finishing period alpaca lysine demand, and the wherein trophic level of lysine is 1.00%.Further, calcium constituent trophic level is 0.55% in the feed;Trophic level of the methionine in feed is 0.40% in the feed.Full price ration formulation provided by the present invention is remarkably improved the whole average daily gain of raising, reduces feed-weight ratio, improves the apparent digestibility of each conventional nutrients.And the feed formula of the present invention is free of antibiotic and other medicated feed additives, and the growth and development of alpaca, feed efficiency can be allowed more preferable.

Description

A kind of perfect compound feed meeting finishing period alpaca lysine demand
Technical field
The invention belongs to perfect compound feed technical fields, and in particular to meet finishing period alpaca lysine demand to a kind of Perfect compound feed.
Background technology
Alpaca (alpaca) is also known as llama, belongs to Mammalia, Camelidae, unique outlook, similar to camel, the bodily form is similar continuous on head Sheep.Alpaca originates in South America, and the whole world is existing ten thousand about more than 400, and Agricultural University Of Shanxi is in 2002 for the first time from Australia Introduce a fine variety alpaca, filled up the blank of Chinese alpaca aquaculture, at present multiple provinces such as Shanxi, Xinjiang, Shandong introduce a fine variety with Raising.Alpaca fibre fineness, intensity and elasticity are superior to wool, and in the world, textile industry is enjoyed high reputation, and woolen is expensive, quilt It is described as " soft gold ".Also it is at the zoo by the favorite animal for display of people with tourist attractions alpaca.Alpaca aquaculture is at me State's herding field belongs to new industry, and development time is shorter, and there are very big gaps compared with external.The cultivation of China's alpaca in recent years Quantity increases sharply, but the report of the research in relation to its nutritional need is less, and the country lacks the nutrition that alpaca feed formulation can refer to Standard.Lysine (lysine, Lys) is the first or second limiting amino acid in animal routine diet, most important physiology Function is to participate in the synthesis of body protein, is added in right amount in diet, the nutrient efficiency etc. that can promote growth of animal, improve diet. Lysine is the important substance for maintaining body vital movement, can improve immunity of organisms, promotes development, lysine is insufficient, sheep The production efficiency and reproductive performance of camel are difficult to normally play, conversely, excessive can lead to poisoning and feed resource waste.China so far It related finishing period alpaca Dietary Lysine optimal level and and daily is had not been reported suitable for pay standard.Therefore, finishing period is inquired into Suitable lysine level in alpaca diet and daily suitable supply amount, provide theoretical foundation for the reasonable preparation of its diet, need Rationally scientifically to screen the type and dosage of Diet Formula.
Invention content
The object of the present invention is to provide a kind of feeds meeting finishing period alpaca lysine demand, are matched with making up alpaca feed System and the insufficient blank of nutrition supply research on standard.
The feed provided by the present invention for meeting finishing period alpaca lysine demand, the trophic level of wherein lysine are 1.00%;
The trophic level of methionine is 0.40% in the feed;
Further, the trophic level of calcium constituent is 0.55% in the feed;
Feed shown in preferably, composition and ratio are as follows:Corn 26.40%, wheat bran 2.00%, dregs of beans 2.00%, rapeseed dregs 2.80%, Cottonseed Meal 2.00%, salt 0.15%, calcium monohydrogen phosphate 0.50%, clover 14.56%, peanut vine 34.55%, corn distiller's dried grain and its soluble matter 3.40%, wheat-middlings 10.00%, DL- METHIONINE 0.15%, DL-lysine 1.00%, premix 1.00%.
The pre-mixing agent of the present invention is usual title in feed companies, and ingredient includes mainly trace element, vitamin etc., Commercial product can be selected, can also be prepared according to nutritional need, premixed feed group of the invention becomes and (contains per kg):VA 7500IU, VD2250IU, VE250IU, Na2SO41.8g, MgO 0.5g, CuSO417.9mg ZnSO450.5mg MnSO4 68.9mg, KI 20mg, Na2SeO3 50mg, CoCl2 38.3mg。
Full price ration formulation provided by the present invention is remarkably improved the whole average daily gain of raising, reduces feed-weight ratio, carries The apparent digestibility of high each conventional nutrients.And the feed formula of the present invention is added without antibiotic and other pharmaceutical feeds Agent can allow the growth and development of alpaca better.
Specific implementation mode
The present invention is described in detail with reference to embodiment.
The screening of 1 finishing period alpaca lysine dosage of embodiment
The screening of 1 finishing period alpaca lysine dosage
1.1 experimental design
It selects the healthy finishing period ram of 11-13 monthly ages, weight close (45.0 ± 2.5) 9 hunchbacked, feeds lysine respectively The different full price mixed ration of content.By Dietary Lysine level be respectively set to 0.50% (1 group), 1.00% (2 groups), 1.50% (3 groups), wherein corn provide 6.3% lysine, and dregs of beans provides 5.3% lysine, and wheat bran provides 1.1% Lysine, rapeseed meal provide 8% lysine, and cotton dregs provide 5% lysine, and remaining lysine content is carried by lysine hydrochloride For.The diet is based on Corn-soybean meal diet, and two kinds of mixing clover, peanut vine coarse fodders are stirred, other nutrition Level is consistent, and diet composition and trophic level are shown in Table 1.Wherein pre-feeding period 7 days, it is positive to try 45 days phases.Alpaca is equal during experiment It is freely eaten and drinks water.
1 diet of table forms and trophic level (air bells dry basis)
1) group of premix becomes:VA7 500IU, VD 2 250IU, VE 250IU, Na2SO41.8g, MgO 0.5g, CuSO417.9mg ZnSO450.5mg MnSO468.9mg, KI 20mg, Na2SeO350mg, CoCl2 38.3mg。
The feeding management of 1.2 alpacas
Alpaca house, hopper, sink etc. were cleaned before on-test, sterilized, daily timing feeding three after on-test It is secondary, it is freely eaten, drinking-water.Alpaca house ground, passageway are cleaned during experiment daily, disinfection is primary weekly, keeps alpaca house clean Health;Sink is scrubbed once daily, keeps hopper clean.
1.3 Testing index and method
1.3.1 alpaca growth performance detects
After each breeding cycle just phase of trying starts, daily accurate recording feeding capacity and surplus doses calculate feed intake.It is each to raise The period is supported respectively at the phase begins, the end of term is in the morning 8:It weighs on an empty stomach before 30 feedings, calculates the average daily gain (ADG) of different phase
Daily dry matter intake=remaining doses * dry matters contain air-dried diet scale of feeding * dry matter contents-daily daily Amount
Alpaca average daily gain=(opisthosoma experimental period, initial body weight-phase weight)/experimental period number of days
Feed-weight ratio=average daily gain/average daily gain
1.3.2 Diet Nutrient apparent digestibility detects
Start digestion trial in just examination end of term phase, alpaca is raised in dedicated metabolic cage endoadaptation 7d, then uses complete It receives excrement method and continuously collects excrement 7d, correct amount adds 10mL10% hydrochloric acid solution fixed nitrogen by every 100g excrement sample, in -20 DEG C of freezings It is to be measured.Daily correct amount record inventory and surplus doses during experiment, and daily surplus material is collected, wind is made in 65 DEG C of drying It is saved backup after dry sample.
Feed and each nutrient content detection of excrement sample:Dry matter (DM) content is measured using oven drying method;Thick protein (CP) contains Amount is measured using Kjeldahl's method (GB/T6432-1994);Crude fat (EE) content is surveyed using remaining method GB/T6433-2006 It is fixed;Acid detergent fiber (ADF) content is measured using NY/T1459-2007 methods;Crude fibre (CF) content uses ANKOM A200i The semi-automatic fibre analyser of type measures;GB/T20806- is respectively adopted in neutral detergent fiber (NDF), calcium (Ca) and phosphorus (P) content 2006, GB/T6436-2002 and GB/T6437-2002) method measurement.
The amount of eating * 100 of the apparent digestibility of lysine=(amount of eating of lysine-fecal calprotectin discharge rate)/lysine
1.3.3 Biochemical Indices In Serum detects
In each breeding cycle closing day, after every alpaca is weighed, empty stomach jugular vein blood collection 5ml stands 3500r/ after 1h Min centrifuge 10min, separation serum be placed in -20 DEG C of refrigerators preserve it is to be measured.Serum glutamic pyruvic transminase, glutamic-oxalacetic transaminease and alkalinity Phosphatase activity, triglycerides, blood glucose, blood calcium, urea, albumin, globulin, total protein content etc. are given birth to using Hitachi 7600 Change fully-automatic analyzer to be detected.
1.4 data processings and statistical analysis
Edit is carried out to test data using Excel2016 softwares, statistical is carried out using 20.0 softwares of SPSS Analysis, is carried out group difference conspicuousness with Duncan's methods and compared, as a result indicated with " average value scholar standard deviation ".
2 results and analysis
Influence of the 2.1 diet difference lysine levels to finishing period alpaca growth performance
Influence of the Dietary Lysine level to alpaca production performance is shown in Table 2.Three groups of alpaca experimental period initial body weights, end of term bodies Weight, feed-weight ratio are without significant difference (P > 0.05);Average daily gain and average daily gain, 1.00% group is significantly higher than 0.50% Group and 1.50% group of (P<0.05), it is significantly higher than 0.50% group of (P for 1.50% group<0.05);1.00% group, 1.50% group of wool production It is significantly higher than 0.50% group of (P<0.05), it is slightly above 1.50% group (P > 0.05) for 1.00% group.
Table 2:Influence of the lysine level to alpaca production performance in diet
Note:Data shoulder mark same letter of going together indicates that difference is not notable (P > 0.05) without shoulder mark, shoulder mark difference small letter Matrix shows that significant difference (P < 0.05), different capitalizations indicate difference extremely significantly (P<0.01).
Influence of the 2.2 diet difference lysine levels to alpaca Diet Nutrient apparent digestibility
Feed lysine level is shown in Table 3 to alpaca Nutrient Apparent Digestibility.1.00% group of alpaca is to crude protein, thick Fat, calcium, crude fibre, acid detergent fiber and lysine apparent digestibility be significantly higher than 0.50% group of (P<0.05), to calcium, Crude fibre, acid detergent fiber apparent digestibility be significantly higher than 1.50% group of (P<0.05).1.50% group of crude fat, thick fibre Peacekeeping lysine apparent digestibility is significantly higher than 0.50% group of (P<0.05).The apparent digestibility three of total phosphorus and neutral detergent fiber Group difference is not notable (P > 0.05).The regression analysis of Dietary Lysine level and thick protein, the apparent digestibility of lysine Equation is respectively:Y=-4.6263x2+10.364x+78.249(R2=1), y=-11.89x2+29.666x+75.746(R2= 1), extreme value calculating learns that lysine additive amount is respectively that 1.12% and 1.25% can obtain thick protein and lysine respectively most High digestibility 84.05% and 94.25%.
Table 3:Influence of the lysine level to alpaca Nutrient Apparent Digestibility
Influence of the 2.3 diet difference lysine levels to alpaca Biochemical Indices In Serum
Influence of the Dietary Lysine level to alpaca Biochemical Indices In Serum is shown in Table 4.Alpaca serum glutamic pyruvic transminase, millet straw turn Without significance difference between ammonia enzyme and 3 groups of alkaline phosphatase activities, serum glucose, urea, total protein, albumin and globulin content Different (P > 0.05), but increase serum triglyceride, glucose, total protein, albumin and globulin content with lysine additive amount It is in raising trend.
Table 4:Influence of the lysine to alpaca Biochemical Indices In Serum in diet
The above results can be seen that average daily gain and the daily ingestion amount of finishing period alpaca with 1.00% Dietary Lysine water Group highest is equalled, unit body surface area wool production 1.00% and 1.5% group are significantly higher than 0.5% group;Nutrient Apparent Digestibility with 1.00% group is best, so that it is determined that finishing period alpaca Dietary Lysine level is 1.00%.
Embodiment 2:The experiment that Qingdao Agricultural University's experiment and demonstration base carries out
In Qingdao Agricultural University's experiment and demonstration base, verified with reference to the level of lysine in embodiment 1, other nutrition Level is consistent, and diet composition and trophic level are shown in Table 5.Wherein pre-feeding period 7 days, it is positive to try 45 days phases.Alpaca is equal during experiment It is freely eaten and drinks water.
5 diet of table forms and trophic level (air bells dry basis)
1) premix be every kilogram include:VA7 500IU, VD 2 250IU, VE 250IU, Na2SO41.8g, MgO 0.5g, CuSO417.9mg ZnSO450.5mg MnSO468.9mg, KI 20mg, Na2SeO350mg, CoCl2 38.3mg。
The feeding management of 1.2 alpacas
Alpaca house, hopper, sink etc. were cleaned before on-test, sterilized, daily timing feeding three after on-test It is secondary, it is freely eaten, drinking-water.Alpaca house ground, passageway are cleaned during experiment daily, disinfection is primary weekly, keeps alpaca house clean Health;Sink is scrubbed once daily, keeps hopper clean.
1.3 Testing index and method
1.3.1 alpaca growth performance detects
After each breeding cycle just phase of trying starts, daily accurate recording feeding capacity and surplus doses calculate feed intake.It is each to raise The period is supported respectively at the phase begins, the end of term is in the morning 8:It weighs on an empty stomach before 30 feedings, calculates the average daily gain (ADG) of different phase
Daily dry matter intake=remaining doses * dry matters contain air-dried diet scale of feeding * dry matter contents-daily daily Amount
Alpaca average daily gain=(opisthosoma experimental period, initial body weight-phase weight)/experimental period number of days
Feed-weight ratio=average daily gain/average daily gain
1.3.2 Diet Nutrient apparent digestibility detects
Start digestion trial in just examination end of term phase, alpaca is raised in dedicated metabolic cage endoadaptation 7d, then uses complete It receives excrement method and continuously collects excrement 7d, correct amount adds 10mL10% hydrochloric acid solution fixed nitrogen by every 100g excrement sample, in -20 DEG C of freezings It is to be measured.Daily correct amount record inventory and surplus doses during experiment, and daily surplus material is collected, wind is made in 65 DEG C of drying It is saved backup after dry sample.
Feed and each nutrient content detection of excrement sample:Dry matter (DM) content is measured using oven drying method;Thick protein (CP) contains Amount is measured using Kjeldahl's method (GB/T6432-1994);Crude fat (EE) content is surveyed using remaining method GB/T6433-2006 It is fixed;Acid detergent fiber (ADF) content is measured using NY/T1459-2007 methods;Crude fibre (CF) content uses ANKOM A200i The semi-automatic fibre analyser of type measures;GB/T20806- is respectively adopted in neutral detergent fiber (NDF), calcium (Ca) and phosphorus (P) content 2006, GB/T6436-2002 and GB/T6437-2002) method measurement.
The amount of eating * 100 of the apparent digestibility of lysine=(amount of eating of lysine-fecal calprotectin discharge rate)/lysine
1.3.3 Biochemical Indices In Serum detects
In each breeding cycle closing day, after every alpaca is weighed, empty stomach jugular vein blood collection 5ml stands 3500r/ after 1h Min centrifuge 10min, separation serum be placed in -20 DEG C of refrigerators preserve it is to be measured.Serum glutamic pyruvic transminase, glutamic-oxalacetic transaminease and alkalinity Phosphatase activity, triglycerides, blood glucose, blood calcium, urea, albumin, globulin, total protein content etc. are given birth to using Hitachi 7600 Change fully-automatic analyzer to be detected.
1.4 data processings and statistical analysis
Edit is carried out to test data using Excel2016 softwares, statistical is carried out using 20.0 softwares of SPSS Analysis, is carried out group difference conspicuousness with Duncan's methods and compared, as a result indicated with " average value scholar standard deviation ".
2 results and analysis
Influence of the 2.1 diet difference lysine levels to finishing period alpaca growth performance
Influence of the diet difference lysine level to finishing period alpaca growth performance index is shown in Table 6.Three groups of alpaca experimental periods Initial body weight, end of term weight, feed-weight ratio are without significant difference (P > 0.05);Average daily gain and average daily gain, 1.00% group aobvious It writes and is higher than 0.50% group and 1.50% group of (P<0.05), it is significantly higher than 0.50% group of (P for 1.50% group<0.05);1.00% group, 1.50% group of wool production is significantly higher than 0.50% group of (P<0.05), it is slightly above 1.50% group (P > 0.05) for 1.00% group.
Table 6:Influence of the lysine level to alpaca production performance in diet
Note:Data shoulder mark same letter of going together indicates that difference is not notable (P > 0.05) without shoulder mark, shoulder mark difference small letter Matrix shows that significant difference (P < 0.05), different capitalizations indicate difference extremely significantly (P<0.01).
Influence of the 2.2 diet difference lysine levels to alpaca Diet Nutrient apparent digestibility
Influence of the feed difference lysine level to alpaca Nutrient Apparent Digestibility is shown in Table 7.1.00% group of alpaca is to diet Thick protein, crude fat, calcium, crude fibre, acid detergent fiber apparent digestibility significantly in 0.50% group of (P<0.05), right Calcium, crude fibre, acid detergent fiber apparent digestibility significantly in 1.50% group of (P<0.05).It is 1.50% group of crude fat, thick Fiber apparent digestibility is significantly higher than 0.50% group of (P<0.05).It is poor between total phosphorus and three groups of the apparent digestibility of neutral detergent fiber Different not notable (P > 0.05).
Table 7:Influence of the lysine level to alpaca Nutrient Apparent Digestibility
Influence of the 2.3 diet difference lysine levels to alpaca Biochemical Indices In Serum
Influence of the diet difference lysine level to alpaca Biochemical Indices In Serum is shown in Table 8.As shown in Table 8, alpaca serum paddy Pyruvic transaminase, glutamic-oxalacetic transaminease and alkaline phosphatase activities, serum glucose, urea, total protein, albumin and globulin content Without significant difference (P > 0.05) between 3 groups, but increase serum triglyceride, glucose, total protein, white with lysine additive amount Albumen and globulin content are in raising trend.
Table 8:Influence of the lysine to alpaca Biochemical Indices In Serum in diet
The above results can be seen that average daily gain and the daily ingestion amount of finishing period alpaca with 1.00% Dietary Lysine water Group highest is equalled, unit body surface area wool production 1.00% and 1.5% group are significantly higher than 0.5% group;Nutrient Apparent Digestibility with 1.00% group is best, it is seen that finishing period alpaca Dietary Lysine level is advisable with 1.00%.
Embodiment 3:The experiment that Qingdao City's livestock technology Demonstration Garden carries out
In Qingdao City's livestock technology Demonstration Garden, verified with reference to the level of lysine in embodiment 1, other trophic level It is consistent, diet composition and trophic level are shown in Table 9.Wherein pre-feeding period 7 days, it is positive to try 45 days phases.Alpaca is free during experiment Feeding and drinking-water.
Table 9:Diet forms and trophic level (air bells dry basis)
1) premix be every kilogram include:VA7 500IU, VD 2 250IU, VE 250IU, Na2SO41.8g, MgO 0.5g, CuSO417.9mg ZnSO450.5mg MnSO468.9mg, KI 20mg, Na2SeO350mg, CoCl2 38.3mg。
The feeding management of 1.2 alpacas
Alpaca house, hopper, sink etc. were cleaned before on-test, sterilized, daily timing feeding three after on-test It is secondary, it is freely eaten, drinking-water.Alpaca house ground, passageway are cleaned during experiment daily, disinfection is primary weekly, keeps alpaca house clean Health;Sink is scrubbed once daily, keeps hopper clean.
1.3 Testing index and method
1.3.1 alpaca growth performance detects
After each breeding cycle just phase of trying starts, daily accurate recording feeding capacity and surplus doses calculate feed intake.It is each to raise The period is supported respectively at the phase begins, the end of term is in the morning 8:It weighs on an empty stomach before 30 feedings, calculates the average daily gain (ADG) of different phase
Daily dry matter intake=air-dry the daily remaining doses × dry matter of diet scale of feeding × dry matter content-daily Content
Alpaca average daily gain=(opisthosoma experimental period, initial body weight-phase weight)/experimental period number of days
Feed-weight ratio=average daily gain/average daily gain
1.3.2 Diet Nutrient apparent digestibility detects
Start digestion trial in just examination end of term phase, alpaca is raised in dedicated metabolic cage endoadaptation 7d, then uses complete It receives excrement method and continuously collects excrement 7d, correct amount adds 10mL10% hydrochloric acid solution fixed nitrogen by every 100g excrement sample, in -20 DEG C of freezings It is to be measured.Daily correct amount record inventory and surplus doses during experiment, and daily surplus material is collected, wind is made in 65 DEG C of drying It is saved backup after dry sample.
Feed and each nutrient content detection of excrement sample:Dry matter (DM) content is measured using oven drying method;Thick protein (CP) contains Amount is measured using Kjeldahl's method (GB/T6432-1994);Crude fat (EE) content is surveyed using remaining method GB/T6433-2006 It is fixed;Acid detergent fiber (ADF) content is measured using NY/T1459-2007 methods;Crude fibre (CF) content uses ANKOM A200i The semi-automatic fibre analyser of type measures;GB/T20806- is respectively adopted in neutral detergent fiber (NDF), calcium (Ca) and phosphorus (P) content 2006, GB/T6436-2002 and GB/T6437-2002) method measurement.
The amount of eating * 100 of the apparent digestibility of lysine=(amount of eating of lysine-fecal calprotectin discharge rate)/lysine
1.3.3 Biochemical Indices In Serum detects
In each breeding cycle closing day, after every alpaca is weighed, empty stomach jugular vein blood collection 5ml stands 3500r/ after 1h Min centrifuge 10min, separation serum be placed in -20 DEG C of refrigerators preserve it is to be measured.Serum glutamic pyruvic transminase, glutamic-oxalacetic transaminease and alkalinity Phosphatase activity, triglycerides, blood glucose, blood calcium, urea, albumin, globulin, total protein content etc. are given birth to using Hitachi 7600 Change fully-automatic analyzer to be detected.
1.4 data processings and statistical analysis
Edit is carried out to test data using Excel2016 softwares, statistical is carried out using 20.0 softwares of SPSS Analysis, is carried out group difference conspicuousness with Duncan's methods and compared, as a result indicated with " average value scholar standard deviation ".
2 results and analysis
Influence of the 2.1 diet difference lysine levels to finishing period alpaca growth performance
Influence of the diet difference lysine level to finishing period alpaca growth performance index is shown in Table 10.Three groups of alpaca experimental periods Initial body weight, end of term weight, feed-weight ratio are without significant difference (P > 0.05);Average daily gain and average daily gain, 1.00% group aobvious It writes and is higher than 0.50% group and 1.50% group of (P<0.05), it is significantly higher than 0.50% group of (P for 1.50% group<0.05);1.00% group, 1.50% group of wool production is significantly higher than 1 group of (P<0.05), it is slightly above 1.50% group (P > 0.05) for 1.00% group.
Influence of the lysine level to alpaca production performance in 10 diet of table
Note:Data shoulder mark same letter of going together indicates that difference is not notable (P > 0.05) without shoulder mark, shoulder mark difference small letter Matrix shows that significant difference (P < 0.05), different capitalizations indicate difference extremely significantly (P<0.01).
Influence of the 2.2 diet difference lysine levels to alpaca Diet Nutrient apparent digestibility
Influence of the feed difference lysine level to alpaca Nutrient Apparent Digestibility is shown in Table 11.1.00% group of alpaca is to diet Thick protein, crude fat, calcium, crude fibre, acid detergent fiber apparent digestibility significantly in 1 group of (P<0.05), to calcium, thick fibre It ties up, the apparent digestibility of acid detergent fiber is significantly in 1.50% group of (P<0.05).1.50% group of crude fat, crude fibre are apparent Digestibility is significantly higher than 0.50% group of (P<0.05).Three group difference of apparent digestibility of total phosphorus and neutral detergent fiber is not notable (P > 0.05).
Influence of 11 lysine level of table to alpaca Nutrient Apparent Digestibility
Influence of the 2.3 diet difference lysine levels to alpaca Biochemical Indices In Serum
Influence of the diet difference lysine level to alpaca Biochemical Indices In Serum is shown in Table 12.As shown in Table 12, alpaca serum Glutamic-pyruvic transaminase, glutamic-oxalacetic transaminease and alkaline phosphatase activities, serum glucose, urea, total protein, albumin and globulin contain Without significant difference (P > 0.05) between measuring 3 groups, but with lysine additive amount increase serum triglyceride, glucose, total protein, Albumin and globulin content are in raising trend.
Influence of the lysine to alpaca Biochemical Indices In Serum in 12 diet of table
The above results can be seen that average daily gain and the daily ingestion amount of finishing period alpaca with 1.00% Dietary Lysine water Group highest is equalled, unit body surface area wool production 1.00% and 1.5% group are significantly higher than 0.5% group;Nutrient Apparent Digestibility with 1.00% group is best, it is seen that finishing period alpaca Dietary Lysine level is advisable with 1.00%.

Claims (6)

1. a kind of feed for finishing period alpaca, which is characterized in that the trophic level of the Dietary Lysine is 1.00%.
2. feed as described in claim 1, which is characterized in that the trophic level of calcium constituent is 0.55% in the feed.
3. feed as claimed in claim 2, which is characterized in that the trophic level of methionine is 0.40% in the feed.
4. feed as described in claim 1, which is characterized in that the composition and ratio of the feed are as follows:Corn 26.40%, wheat bran 2.00%, dregs of beans 2.00%, rapeseed dregs 2.80%, Cottonseed Meal 2.00%, salt 0.15%, calcium monohydrogen phosphate 0.50%, clover 14.56%, peanut vine 34.55%, corn distiller's dried grain and its soluble matter 3.40%, wheat-middlings 10.00%, DL- eggs Propylhomoserin 0.15%, DL-lysine 1.00%, premix 1.00%.
5. feed as claimed in claim 4, which is characterized in that the feed pre-mixing material contains following component per kg:VA 7500IU, VD2250IU, VE250IU, Na2SO41.8g, MgO 0.5g, CuSO417.9mg ZnSO450.5mg MnSO4 68.9mg, KI 20mg, Na2SeO3 50mg, CoCl2 38.3mg。
6. a kind of method for breeding of finishing period alpaca, which is characterized in that the method for breeding be feed it is described in claim 1 Feed.
CN201810443427.7A 2018-05-10 2018-05-10 A kind of perfect compound feed meeting finishing period alpaca lysine demand Withdrawn CN108617861A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114145384A (en) * 2021-11-29 2022-03-08 中农华威生物制药(湖北)有限公司 Fermentation method for increasing lysine content of traditional Chinese medicine feed additive

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114145384A (en) * 2021-11-29 2022-03-08 中农华威生物制药(湖北)有限公司 Fermentation method for increasing lysine content of traditional Chinese medicine feed additive
CN114145384B (en) * 2021-11-29 2024-02-13 中农华威生物制药(湖北)有限公司 Fermentation method for increasing lysine content of traditional Chinese medicine feed additive

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