CN108887487A - A kind of perfect compound feed meeting adult alpaca mineral matter element calcium demand - Google Patents

A kind of perfect compound feed meeting adult alpaca mineral matter element calcium demand Download PDF

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CN108887487A
CN108887487A CN201810751998.7A CN201810751998A CN108887487A CN 108887487 A CN108887487 A CN 108887487A CN 201810751998 A CN201810751998 A CN 201810751998A CN 108887487 A CN108887487 A CN 108887487A
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calcium
alpaca
feed
group
diet
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王述柏
郝薪云
谭子超
于超
刘晓龙
周传凤
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Qingdao Agricultural University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/10Feeding-stuffs specially adapted for particular animals for ruminants
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/26Compounds containing phosphorus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/30Oligoelements
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The present invention provides a kind of perfect compound feed for meeting adult alpaca mineral matter element calcium demand, and the additive amount of Mineral Elements in Jadeite Shellfish calcium is 0.77%.Feed provided by the present invention, a kind of composition and ratio are as follows:Corn 30.02%, dregs of beans 2.50%, clover 16.44%, peanut vine 48.3%, salt 0.15%, calcium monohydrogen phosphate 1.26%, potassium dihydrogen phosphate 0.33%, premix 1%.Full price ration formulation provided by the present invention is remarkably improved the whole average daily gain of raising, reduces feed-weight ratio, improves the apparent digestibility of each conventional nutrients.And feed formula of the invention is free of antibiotic and other drugs feed addictive, and the adult production performance of alpaca, price of deed etc. can be allowed to reach optimum level.

Description

A kind of perfect compound feed meeting adult alpaca mineral matter element calcium demand
Technical field
The invention belongs to technical field of animal, and in particular to a kind of to meet alpaca mineral matter element calcium demand of growing up Perfect compound feed.
Background technique
Alpaca (alpaca) category Mammalia, Artiodactyla, Camelidae, yamma category originate in the Andes of South America With Alps contour ground region, there is good adaptability to High aititude and high and cold, drought environment.Alpaca fibre is in state Be known as on border " soft gold, meat, milk also have very high nutritive value, since Agricultural University Of Shanxi in 2002 introduces alpaca, China's alpaca industry is quickly grown, it has also become a novel industry and rising industry in China's animal husbandry.Alpaca disposition is docile, resists Inverse property and premunition are strong, and resistance to crude feed drafts a document food small ruminant.Report so far in relation to ruminant calcium trophic level is more, But less, the corresponding data parameters of different physiological stage alpaca nutritional requirements shortages are reported the research of alpaca, sheep in production There are blindness and randomness for the feed formulation of camel.With the fast development of China's alpaca large-scale cultivation, establishes and be suitble to China The demand of the alpaca trophic level system of natural environment is increasingly urgent to.But at present both at home and abroad in terms of related alpaca calcium nutritional need Research has not been reported, and the feed formulation of alpaca lacks relevant reference standard in production.Calcium is the important of animal skeleton and tooth Component part, it is closely bound up with the physiological activities such as nerve conduction, energetic supersession, suitable calcium level is provided in feed to remaining dynamic The normal growth and development of object is most important.Calcium excessively may make certain microelements invalid in daily ration;Conversely, calcium deficiency is easily led Young animal suffers from rickets, adults suffer from osteoporosis for cause.But at present still for calcium ion demand in alpaca feed Referring to the standard of other cultivated animals, lead to the nutritional need of current alpaca feed and inadaptable adult alpaca.
Summary of the invention
The object of the present invention is to provide a kind of perfect compound feeds for meeting adult alpaca mineral matter element calcium demand, thus It makes up alpaca feed formula and studies insufficient blank.
The feed provided by the present invention for meeting adult alpaca mineral matter element calcium demand, the battalion of Mineral Elements in Jadeite Shellfish calcium Supporting horizontal is 0.77%;
The mineral matter element calcium is provided by calcium monohydrogen phosphate;
The specific record of one kind as embodiment, provided feed, composition and ratio are as follows:Corn 30.02%, beans The dregs of rice 2.50%, clover 16.44%, peanut vine 48.3%, salt 0.15%, calcium monohydrogen phosphate 1.26%, potassium dihydrogen phosphate 0.33%, Premix 1%.
Used premix group becomes (every kg contains):VA7500IU, VD2250IU, VE250 IU, Na2SO41.8g MgO 0.5g, CuSO417.9mg ZnSO450.5mg MnSO468.9mg, KI 20mg, Na2SeO3 50mg, CoCl2 38.3mg。
Full price ration formulation provided by the present invention is remarkably improved the whole average daily gain of raising, reduces feed-weight ratio, mentions The apparent digestibility of high each conventional nutrients.And feed formula of the invention is free of antibiotic and other drugs feed addition Agent can allow the growth and development of alpaca better.
Specific embodiment
The present invention detects feeding effect by feeding experiment and digestion trial, obtains alpaca diet mineral matter element calcium Optimum content, reference frame is provided for the reasonable preparation of its diet.
The present invention is described in detail below with reference to embodiment.
The screening of the adult alpaca mineral matter element calcium dosage of embodiment 1
1.1 select the healthy adult ram of 28-30 monthly age, weight close (60.88 ± 1.75kg) 4 hunchbacked, feed respectively The different full price mixed ration of calcium content.Advance referring to the diet nutritional requirements of the ruminants such as sheep, and in on-test Row is pre- to raise test, determine full price mixed ration with corn-soybean meal basis, two kinds of mixing clover, peanut vine coarse fodders be stirred and At other trophic level are consistent.Diet calcium level is respectively set to 0.57%, 0.67%, 0.77%, 0.87%, Middle calcium monohydrogen phosphate provides 17% calcium constituent, and corn provides 55% calcium constituent, and dregs of beans provides 11% calcium constituent, and peanut vine mentions For 11% calcium constituent, clover provides 6% calcium constituent.Diet composition and trophic level are shown in Table 1.Test uses 4 × 4 Latin squares Experimental design carries out the feeding experiment in 4 periods, and each cycle 27 days, wherein pre-feeding period 7 days, positive to try the phase 20 days.
Table 1:The content of diet composition (air bells dry basis) and calcium constituent of the invention
1) group of premix becomes (every kg contains):VA 7 500 IU, VD 2 250 IU, VE 250 IU, Na2SO4 1.8g, MgO 0.5g, CuSO417.9mg ZnSO450.5mg MnSO468.9mg, KI 20mg, Na2SeO350mg, CoCl2 38.3mg。
The feeding management of 1.2 alpacas
Alpaca house, hopper, sink etc. were cleaned before on-test, sterilized, daily timing feeding three after on-test It is secondary, it is freely eaten, drinking-water.Alpaca house ground, passageway are cleaned during test daily, disinfection is primary weekly, keeps alpaca house clean Health;Sink is scrubbed once daily, keeps hopper clean.
1.3 Testing index and method
1.3.1 alpaca growth performance detects
After each breeding cycle just phase of trying starts, daily accurate recording feeding capacity and surplus doses calculate feed intake.Each feeding The period is supported respectively at the phase begins, the end of term is in the morning 8:It weighs on an empty stomach before 30 feedings, calculates the average daily gain (ADG) of different phase
Daily dry matter intake=air-dry the daily remaining doses × dry matter of diet scale of feeding × dry matter content-daily Content
Alpaca average daily gain=(opisthosoma experimental period, initial body weight-phase weight)/experimental period number of days
Feed-weight ratio=average daily gain/average daily gain
1.3.2 Diet Nutrient apparent digestibility detects
Start digestion trial in just examination end of term phase, alpaca is raised in adapting to 7d in dedicated metabolic cage, then using complete It receives excrement method and continuously collects excrement 7d, correct amount adds 10mL10% hydrochloric acid solution fixed nitrogen by every 100g excrement sample, freezes in -20 DEG C It is to be measured.Daily correct amount record inventory and surplus doses during test, and daily surplus material is collected, wind is made in 65 DEG C of drying It is saved backup after dry sample.
The detection of the nutrient content of feed and excrement sample:Dry matter (DM) content is measured using oven drying method;Thick protein (CP) contains Amount is measured using Kjeldahl's method (GB/T6432-1994);Crude fat (EE) content is surveyed using remnants method GB/T6433-2006 It is fixed;Acid detergent fiber (ADF) content is measured using NY/T1459-2007 method;Crude fibre (CF) content uses ANKOM A200i The semi-automatic fibre analyser measurement of type;GB/T20806- is respectively adopted in neutral detergent fiber (NDF), calcium (Ca) and phosphorus (P) content 2006, GB/T6436-2002 and GB/T6437-2002) method measurement.
The apparent digestibility of calcium=(amount of eating of calcium-fecal calprotectin the discharge rate)/calcium amount of eating × 100%
1.3.3 Biochemical Indices In Serum detects
After each breeding cycle closing day, every alpaca weighing, empty stomach jugular vein blood collection 5ml stands 3500r/ after 1h Min be centrifuged 10min, separation serum be placed in -20 DEG C of refrigerators save it is to be measured.Serum glutamic pyruvic transminase, glutamic-oxalacetic transaminease and alkalinity Phosphatase activity, triglycerides, blood glucose, blood calcium, urea, albumin, globulin, total protein content etc. are raw using Hitachi 7600 Change fully-automatic analyzer to be detected.
1.4 data processings and statistical analysis
Edit is carried out to test data using Excel2016 software, statistical is carried out using 20.0 software of SPSS Analysis, is carried out group difference conspicuousness with Duncan's method and compared, as a result indicated with " average value scholar standard deviation ".
2 results and analysis
Influence of the 2.1 diet difference calcium levels to adult alpaca growth performance
Influence of the diet calcium level to ewe camel apparent digestibility is shown in Table 2..Feed-weight ratio with 0.57% group of highest, with 0.87% is all significantly higher than 0.77% group of (P<0.05).Average 0.57%, 0.67% two group of feed intake is slightly above 0.77% combination 0.87% group (P > 0.05).Average daily gain with 0.77% group of highest, but with the difference on other three groups of inanimate object statistical significances Different conspicuousness (P > 0.05).
Table 2:Influence of the calcium level to ewe camel weight and feed-weight ratio
Note:Data shoulder mark same letter of going together indicates that difference is not significant (P > 0.05) without shoulder mark, shoulder mark difference small letter Matrix shows significant difference (P < 0.05) that different capitalizations indicate the extremely significant (P of difference<0.01).
Influence of the 2.2 diet difference calcium levels to alpaca Diet Nutrient apparent digestibility
Influence of the diet calcium level to ewe camel apparent digestibility is shown in Table 3.Crude protein, phosphorus and neutral detergent fiber it is apparent Digestibility, 0.77% group is significantly higher than 0.87% group of (P<0.05).The apparent digestibility of calcium, 0.77% group of highest, and it is extremely significant Higher than 0.57%, 0.67% group (P<0.01).The digestibility of acid detergent fiber, 0.77% group of highest, be significantly higher than other three Group (P<0.05).Crude fat and coarse-fibred apparent digestibility are not significantly different (P > 0.05) between four groups.
Table 3:Influence of the calcium level to ewe camel diet nutriment apparent digestibility
Influence of the 2.3 diet difference calcium levels to alpaca Biochemical Indices In Serum
Influence of the diet calcium level to ewe camel Biochemical Indices In Serum is shown in Table 4.Third turn of ewe camel alkaline phosphatase, paddy ammonia Without significant difference (P > 0.05) between enzyme, glutamic-oxalacetic transaminease activity each group.The total calcium of triglycerides, blood glucose, blood, serium inorganic phosphorus, urea etc. Also without significant difference (P > 0.05) between content each group.Total protein content with 0.77% group of highest, be significantly higher than 0.67% group and 0.87% group of (P<0.05).Without significant difference between parathyroid hormone cellulose content each group, but with the raising of calcium concentration, content is in Reveal the trend (P > 0.05) gradually increased.
Table 4:Influence of the calcium level to ewe camel Biochemical Indices In Serum
The above results can be seen that 0.77% group of adult ewe camel feed-weight ratio of diet calcium level be substantially less than 0.57% group and 0.87% group, and 0.77% group of average daily gain is also higher than other three groups;It is increased with diet calcium level, alpaca is to the apparent of calcium Digestibility significantly increases, and 0.77% group and 0.87% group is much higher than 0.57% and 0.67% group;Crude protein, total phosphorus, acidic cleaning The apparent digestibility of fiber and alkaline washing fiber is also with 0.77% group of highest;Crude fat and coarse-fibred apparent digestibility each group Difference is not significant between though, and 0.77% group has higher than other group trend;The diet calcium level of 0.57%-0.87% is to alpaca 11 Biochemical Indices In Serum does not make significant difference.Comprehensive analysis, finishing period ram camel diet calcium level are preferred with 0.77%.
Embodiment 2:The test in Qingdao Agricultural University's experiment and demonstration base
In Qingdao Agricultural University's experiment and demonstration base, verified referring to calcium level set in embodiment 1, other nutrition Level is consistent, and diet composition and trophic level are shown in Table 5.Test is designed using 4 × 4 latin square experiments, carries out 4 periods Feeding experiment, each cycle 27 days, wherein pre-feeding period 7 days, positive to try the phase 20 days.
Table 5:Diet composition and trophic level (air bells dry basis)
1) premix provides for every kilogram:VA 7 500 IU, VD 2 250 IU, VE 250 IU, Na2SO41.8g, MgO 0.5g, CuSO417.9mg ZnSO450.5mg MnSO468.9mg, KI 20mg, Na2SeO350mg, CoCl2 38.3mg。
The feeding management of 1.2 alpacas
Alpaca house, hopper, sink etc. were cleaned before on-test, sterilized, daily timing feeding three after on-test It is secondary, it is freely eaten, drinking-water.Alpaca house ground, passageway are cleaned during test daily, disinfection is primary weekly, keeps alpaca house clean Health;Sink is scrubbed once daily, keeps hopper clean.
1.3 Testing index and method
1.3.1 alpaca growth performance detects
After each breeding cycle just phase of trying starts, daily accurate recording feeding capacity and surplus doses calculate feed intake.Each feeding The period is supported respectively at the phase begins, the end of term is in the morning 8:It weighs on an empty stomach before 30 feedings, calculates the average daily gain (ADG) of different phase
Daily dry matter intake=air-dry the daily remaining doses × dry matter of diet scale of feeding × dry matter content-daily Content
Alpaca average daily gain=(opisthosoma experimental period, initial body weight-phase weight)/experimental period number of days
Feed-weight ratio=average daily gain/average daily gain
1.3.2 Diet Nutrient apparent digestibility detects
Start digestion trial in just examination end of term phase, alpaca is raised in adapting to 7d in dedicated metabolic cage, then using complete It receives excrement method and continuously collects excrement 7d, correct amount adds 10mL10% hydrochloric acid solution fixed nitrogen by every 100g excrement sample, freezes in -20 DEG C It is to be measured.Daily correct amount record inventory and surplus doses during test, and daily surplus material is collected, wind is made in 65 DEG C of drying It is saved backup after dry sample.
The detection of the nutrient content of feed and excrement sample:Dry matter (DM) content is measured using oven drying method;Thick protein (CP) contains Amount is measured using Kjeldahl's method (GB/T6432-1994);Crude fat (EE) content is surveyed using remnants method GB/T6433-2006 It is fixed;Acid detergent fiber (ADF) content is measured using NY/T1459-2007 method;Crude fibre (CF) content uses ANKOM A200i The semi-automatic fibre analyser measurement of type;GB/T20806- is respectively adopted in neutral detergent fiber (NDF), calcium (Ca) and phosphorus (P) content 2006, GB/T6436-2002 and GB/T6437-2002) method measurement.
The apparent digestibility of calcium=(amount of eating of calcium-fecal calprotectin the discharge rate)/calcium amount of eating × 100%
1.3.3 Biochemical Indices In Serum detects
After each breeding cycle closing day, every alpaca weighing, empty stomach jugular vein blood collection 5ml stands 3500r/ after 1h Min be centrifuged 10min, separation serum be placed in -20 DEG C of refrigerators save it is to be measured.Serum glutamic pyruvic transminase, glutamic-oxalacetic transaminease and alkalinity Phosphatase activity, triglycerides, blood glucose, blood calcium, urea, albumin, globulin, total protein content etc. are raw using Hitachi 7600 Change fully-automatic analyzer to be detected.
1.4 data processings and statistical analysis
Edit is carried out to test data using Excel2016 software, statistical is carried out using 20.0 software of SPSS Analysis, is carried out group difference conspicuousness with Duncan's method and compared, as a result indicated with " average value scholar standard deviation ".
2 results and analysis
Influence of the 2.1 diet difference calcium levels to adult alpaca growth performance
Influence of the diet calcium level to ewe camel apparent digestibility is shown in Table 6.Feed-weight ratio is with 0.57% group of highest, with 0.87% All it is significantly higher than 0.77% group of (P<0.05).Average 0.57%, 0.67% two group of feed intake is slightly above 0.77% combination 0.87% Group (P > 0.05).Average daily gain with 0.77% group of highest, but with the significant difference on other three groups of inanimate object statistical significances Property (P > 0.05).
Table 6:Influence of the calcium level to ewe camel weight and feed-weight ratio
Note:Data shoulder mark same letter of going together indicates that difference is not significant (P > 0.05) without shoulder mark, shoulder mark difference small letter Matrix shows significant difference (P < 0.05) that different capitalizations indicate the extremely significant (P of difference<0.01).
Influence of the 2.2 diet difference calcium levels to alpaca Diet Nutrient apparent digestibility
Influence of the diet calcium level to ewe camel apparent digestibility is shown in Table 7.Crude protein, phosphorus and neutral detergent fiber it is apparent Digestibility, 0.77% group is significantly higher than 0.87% group of (P<0.05).The apparent digestibility of calcium, 0.77% group of highest, and it is extremely significant Higher than 0.57%, 0.67% group (P<0.01).The digestibility of acid detergent fiber, 0.77% group of highest, be significantly higher than other three Group (P<0.05).Crude fat and coarse-fibred apparent digestibility are not significantly different (P > 0.05) between four groups.
Table 7:Influence of the calcium level to ewe camel diet nutriment apparent digestibility
Influence of the 2.3 diet difference calcium levels to alpaca Biochemical Indices In Serum
Influence of the diet calcium level to ewe camel Biochemical Indices In Serum is shown in Table 8.Third turn of ewe camel alkaline phosphatase, paddy ammonia Without significant difference (P > 0.05) between enzyme, glutamic-oxalacetic transaminease activity each group.The total calcium of triglycerides, blood glucose, blood, serium inorganic phosphorus, urea etc. Also without significant difference (P > 0.05) between content each group.Total protein content with 0.77% group of highest, be significantly higher than 0.67% group and 0.87% group of (P<0.05).Without significant difference between parathyroid hormone cellulose content each group, but with the raising of calcium concentration, content is in Reveal the trend (P > 0.05) gradually increased.
Table 8:Influence of the calcium level to ewe camel Biochemical Indices In Serum
The above results can be seen that 0.77% group of adult ewe camel feed-weight ratio of diet calcium level be substantially less than 0.57% group and 0.87% group, and 0.77% group of average daily gain is also higher than other three groups;It is increased with diet calcium level, alpaca is to the apparent of calcium Digestibility significantly increases, and 0.77% group and 0.87% group is much higher than 0.57% and 0.67% group;Crude protein, total phosphorus, acidic cleaning The apparent digestibility of fiber and alkaline washing fiber is also with 0.77% group of highest;Crude fat and coarse-fibred apparent digestibility each group Difference is not significant between though, and 0.77% group has higher than other group trend;The diet calcium level of 0.57%-0.87% is to alpaca 11 Biochemical Indices In Serum does not make significant difference.Comprehensive analysis, finishing period ram camel diet calcium level are preferred with 0.77%.
Embodiment 3:
In Qingdao City's livestock technology Demonstration Garden, verified referring to calcium level set in embodiment 1, other trophic level It is consistent, diet composition and trophic level are shown in Table 9.Test is designed using 4 × 4 latin square experiments, carries out the raising in 4 periods Test, each cycle 27 days, wherein pre-feeding period 7 days, positive to try the phase 20 days.
Table 9:Diet composition and trophic level (air bells dry basis)
1) premix be every kilogram include:VA 7 500 IU, VD 2 250 IU, VE 250 IU, Na2SO41.8g MgO 0.5g, CuSO417.9mg ZnSO450.5mg MnSO468.9mg, KI 20mg, Na2SeO350mg, CoCl2 38.3mg。
The feeding management of 1.2 alpacas
Alpaca house, hopper, sink etc. were cleaned before on-test, sterilized, daily timing feeding three after on-test It is secondary, it is freely eaten, drinking-water.Alpaca house ground, passageway are cleaned during test daily, disinfection is primary weekly, keeps alpaca house clean Health;Sink is scrubbed once daily, keeps hopper clean.
1.3 Testing index and method
1.3.1 alpaca growth performance detects
After each breeding cycle just phase of trying starts, daily accurate recording feeding capacity and surplus doses calculate feed intake.Each feeding The period is supported respectively at the phase begins, the end of term is in the morning 8:It weighs on an empty stomach before 30 feedings, calculates the average daily gain (ADG) of different phase
Daily dry matter intake=air-dry the daily remaining doses × dry matter of diet scale of feeding × dry matter content-daily Content
Alpaca average daily gain=(opisthosoma experimental period, initial body weight-phase weight)/experimental period number of days
Feed-weight ratio=average daily gain/average daily gain
1.3.2 Diet Nutrient apparent digestibility detects
Start digestion trial in just examination end of term phase, alpaca is raised in adapting to 7d in dedicated metabolic cage, then using complete It receives excrement method and continuously collects excrement 7d, correct amount adds 10mL10% hydrochloric acid solution fixed nitrogen by every 100g excrement sample, freezes in -20 DEG C It is to be measured.Daily correct amount record inventory and surplus doses during test, and daily surplus material is collected, wind is made in 65 DEG C of drying It is saved backup after dry sample.
The detection of the nutrient content of feed and excrement sample:Dry matter (DM) content is measured using oven drying method;Thick protein (CP) contains Amount is measured using Kjeldahl's method (GB/T6432-1994);Crude fat (EE) content is surveyed using remnants method GB/T6433-2006 It is fixed;Acid detergent fiber (ADF) content is measured using NY/T1459-2007 method;Crude fibre (CF) content uses ANKOM A200i The semi-automatic fibre analyser measurement of type;GB/T20806- is respectively adopted in neutral detergent fiber (NDF), calcium (Ca) and phosphorus (P) content 2006, GB/T6436-2002 and GB/T6437-2002) method measurement.
The apparent digestibility of calcium=(amount of eating of calcium-fecal calprotectin the discharge rate)/calcium amount of eating × 100
1.3.3 Biochemical Indices In Serum detects
After each breeding cycle closing day, every alpaca weighing, empty stomach jugular vein blood collection 5ml stands 3500r/ after 1h Min be centrifuged 10min, separation serum be placed in -20 DEG C of refrigerators save it is to be measured.Serum glutamic pyruvic transminase, glutamic-oxalacetic transaminease and alkalinity Phosphatase activity, triglycerides, blood glucose, blood calcium, urea, albumin, globulin, total protein content etc. are raw using Hitachi 7600 Change fully-automatic analyzer to be detected.
1.4 data processings and statistical analysis
Edit is carried out to test data using Excel2016 software, statistical is carried out using 20.0 software of SPSS Analysis, is carried out group difference conspicuousness with Duncan's method and compared, as a result indicated with " average value scholar standard deviation ".
2 results and analysis
Influence of the 2.1 diet difference calcium levels to adult alpaca growth performance
Influence of the diet calcium level to ewe camel apparent digestibility is shown in Table 10.Feed-weight ratio with 0.57% group of highest, with 0.87% is all significantly higher than 0.77% group of (P<0.05).Average 0.57%, 0.67% two group of feed intake is slightly above 0.77% combination 0.87% group (P > 0.05).Average daily gain with 0.77% group of highest, but with the difference on other three groups of inanimate object statistical significances Different conspicuousness (P > 0.05).
Table 10:Influence of the calcium level to ewe camel weight and feed-weight ratio
Note:Data shoulder mark same letter of going together indicates that difference is not significant (P > 0.05) without shoulder mark, shoulder mark difference small letter Matrix shows significant difference (P < 0.05) that different capitalizations indicate the extremely significant (P of difference<0.01).
Influence of the 2.2 diet difference calcium levels to alpaca Diet Nutrient apparent digestibility
Influence of the diet calcium level to ewe camel apparent digestibility is shown in Table 11.The table of crude protein, phosphorus and neutral detergent fiber Digestibility is seen, 0.77% group is significantly higher than 0.87% group of (P<0.05).The apparent digestibility of calcium, 0.77% group of highest, and it is extremely aobvious It writes and is higher than 0.57%, 0.67% group of (P<0.01).The digestibility of acid detergent fiber, 0.77% group of highest, is significantly higher than other Three groups of (P<0.05).Crude fat and coarse-fibred apparent digestibility are not significantly different (P > 0.05) between four groups.
Table 11:Influence of the calcium level to ewe camel diet nutriment apparent digestibility
Influence of the 2.3 diet difference calcium levels to alpaca Biochemical Indices In Serum
Influence of the diet calcium level to ewe camel Biochemical Indices In Serum is shown in Table 12.Third turn of ewe camel alkaline phosphatase, paddy ammonia Without significant difference (P > 0.05) between enzyme, glutamic-oxalacetic transaminease activity each group.The total calcium of triglycerides, blood glucose, blood, serium inorganic phosphorus, urea etc. Also without significant difference (P > 0.05) between content each group.Total protein content with 0.77% group of highest, be significantly higher than 0.67% group and 0.87% group of (P<0.05).Without significant difference between parathyroid hormone cellulose content each group, but with the raising of calcium concentration, content is in Reveal the trend (P > 0.05) gradually increased.
Table 12:Influence of the calcium level to ewe camel Biochemical Indices In Serum
The above results can be seen that 0.77% group of adult ewe camel feed-weight ratio of diet calcium level be substantially less than 0.57% group and 0.87% group, and 0.77% group of average daily gain is also higher than other three groups;It is increased with diet calcium level, alpaca is to the apparent of calcium Digestibility significantly increases, and 0.77% group and 0.87% group is much higher than 0.57% and 0.67% group;Crude protein, total phosphorus, acidic cleaning The apparent digestibility of fiber and alkaline washing fiber is also with 0.77% group of highest;Crude fat and coarse-fibred apparent digestibility each group Difference is not significant between though, and 0.77% group has higher than other group trend;The diet calcium level of 0.57%-0.87% is to alpaca 11 Biochemical Indices In Serum does not make significant difference.Comprehensive analysis, finishing period ram camel diet calcium level are preferred with 0.77%.

Claims (4)

1. a kind of perfect compound feed for meeting adult alpaca mineral matter element calcium demand, which is characterized in that in the feed The trophic level of mineral matter element calcium is 0.77%.
2. perfect compound feed as described in claim 1, which is characterized in that the mineral matter element calcium is by calcium monohydrogen phosphate It provides.
3. perfect compound feed as described in claim 1, which is characterized in that the feed, composition and ratio are as follows:It is beautiful Rice 30.02%, dregs of beans 2.50%, clover 16.44%, peanut vine 48.3%, salt 0.15%, calcium monohydrogen phosphate 1.26%, phosphoric acid Potassium dihydrogen 0.32%, premix 1%.
4. perfect compound feed as claimed in claim 3, which is characterized in that the every kg of the premix contains following group Point:VA7500IU, VD2250IU, VE250IU, Na2SO41.8g, MgO 0.5g, CuSO417.9mg ZnSO450.5mg, MnSO468.9mg, KI 20mg, Na2SeO3 50mg, CoCl2 38.3mg。
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CN104489256A (en) * 2014-11-30 2015-04-08 王旭 Concentrate feed for alpaca
CN104719676A (en) * 2015-04-03 2015-06-24 沈阳旭达冠养殖厂 Complete pellet feed for fattening barn feeding lambs
CN105124271A (en) * 2015-08-28 2015-12-09 明光市田地农产品贸易有限公司 Low-cost corn straw feed for alpacas and preparation method of low-cost corn straw feed

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