CN102352314B - Composite bacillus cottonseed meal fermenting agent and preparation method thereof - Google Patents
Composite bacillus cottonseed meal fermenting agent and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a composite bacillus cottonseed meal fermenting agent and a preparation method thereof. The preparation method comprises the following steps of: performing expanding culture on strains of bacillus subtilis, bacillus natto, brevibacillus laterosporus and bacillus coagulans by a liquid fermentation method; inoculating into a fermentation tank to ferment; adding a carrier to adsorb; drying at low temperature; and mixing in a ratio to prepare the composite bacillus cottonseed meal fermenting agent, the total microbial content of the fermenting agent is more than or equal to 100*108 CFU/G. The composite bacillus cottonseed meal fermenting agent can effectively inhibit growth of miscellaneous bacteria in the fermentation process of the cottonseed meal, reduce the free gossypol content of the cottonseed meal and increase the protein content and the free amino acid content of the cottonseed meal, and has the characteristics of simple preparation process, stable and reliable production quality, low cost and the like.
Description
Technical field:
The invention belongs to the microbial fermentation technology field, relate to a kind of preparation method of cotton dregs starter, particularly a kind of preparation method of composite bacillus cotton dregs starter.
Background technology:
Cotton dregs are the by products after the cottonseed oil expression, account for China and produce about 30% of various grouts total amounts per year.Cotton dregs are a kind of good protein feed resources, and its output is nutritious greatly.Cottonseed meal protein contnt as feedstuff raw material is generally 44.32% at present, is only second to the protein contnt 49.48% of dregs of beans, and is higher than the protein contnt 36.04% of rapeseed meal.Can obtain 17 seed amino acids after the cotton dregs hydrolysis, wherein lysine content is lower, is 1.4-2.13%, and arginine content is higher, is 3.94-4.98%, and the ratio of Methionin and l-arginine was considerably beyond 100: 120 ideal value.From 8 kinds of indispensable amino acids, cottonseed protein and dregs of beans albumen are more approaching, see that from VITAMINs and mineral substance aspect cotton dregs contain abundant vitamin E and vitamin B group, and phosphorus content is more than the 0.83-1.04%.Therefore, cotton dregs are one of important vegetable protein resources.But owing to contain the deleterious free gossypol of animal in the cotton dregs, thereby influence its feeding amount, it is not very high causing its ratio shared in protein raw material.
At present, domestic detoxification to cotton cake dregs mainly is to adopt the biological fermentation detoxicity method, and the microbe species of use is different because of different fermentations technology.The ultimate principle of utilizing the detoxification of microbial fermentation cotton cake dregs is to utilize mikrobe in the growth metabolism process, to produce the free gossypol in some enzyme degraded cotton cake dregs, thereby reaches the purpose of detoxification.If about microbial host yeast that domestic cotton dregs microbial detoxification adopted, milk-acid bacteria, EM microbial inoculum, mould (aspergillus oryzae, black mold, Aspergillus batatae, monascus etc.), subtilis etc.Because above-mentioned bacterial classification can not produce gemma during the fermentation mostly, exist the fermenting agent viable count of producing not high, the preservation time does not grow and ferment effect is difficult to stablize, and perhaps fermented bacterium is single, shortcomings such as complex manufacturing and production cost height.For adopting bacillus natto, Bacillus coagulans, side gemma spore bacillus and subtilis to form the technology that the complex ferment microbial inoculum fermented cotton dregs of rice carry out detoxification treatment, do not see bibliographical information both at home and abroad.
Summary of the invention:
Technical problem to be solved by this invention is: to the deficiency of above-mentioned prior art; A kind of composite bacillus cotton dregs starter and preparation method thereof is provided, and this starter preparation technology is simple, cost and energy consumption is low, flexibility strong, the preservation time is long, be easy to produce in batches, detoxification efficiency is stable and effective.
In order to solve the problems of the technologies described above, the technical scheme that the present invention adopted is: a kind of composite bacillus cotton dregs starter contains Bacillus coagulans (Bacillus coagulans) 3-5 * 10 in this starter
8CFU/g, side gemma spore bacillus (Bacillus lateraporus) 25-35 * 10
8CFU/g, bacillus natto (Bacillus natto) 30-40 * 10
8CFU/g, and subtilis (Bacillus subtilis) 25-35 * 10
8CFU/g, total count>=100 * 10
8CFU/g.
The preparation method of above-mentioned composite bacillus cotton dregs starter comprises the steps:
(1) preparation liquid spawn: get respectively subtilis, side gemma spore bacillus, bacillus natto, and each 1 strain of Bacillus coagulans inoculate a ring slant strains cell respectively in four liquid spawn culture mediums; At 37 ℃-40 ℃; Under the 160-200r/min condition, shaking table shaking culture 48-60h makes the cell gemma rate of formation in each seed liquor reach 90-95%; Handle 10-15min in 80 ℃ of waters bath with thermostatic control again, obtain four kinds of liquid spawns;
The aforesaid liquid bacterium culture medium consists of to add in every premium on currency has 30-40g glucose, 10-15g soybean cake powder, 2-6g (NH
4)
2SO
4, 2-4g KH
2PO
4, 1-2.5g MgSO
47H
2O, 3-5g NaCl, and 2-4g CaCO
3, original ph is 7.2-7.5.
(2) fermentation: the inoculum size of above-mentioned each liquid spawn by 3-6% (V/V) is inoculated in respectively in the corresponding fermention medium,, cultivates 48-72h under the stirring velocity 160-180r/min condition at culture temperature 30-40 ℃;
Wherein: the fermention medium of Bacillus coagulans consists of to add in every premium on currency has 15-25g wheat bran, 7-10g soybean cake powder, 5-10g steeping water, 2-4g KH
2PO
4, 1-2.5g MgSO
47H
2O, 0.1-0.3gMnSO
4H
2O, and 3-5gCaCO
3, original ph is 7.2-7.5.
The fermention medium of side gemma spore bacillus and bacillus natto consists of to add in every premium on currency has 25-35g glucose, 10-20g soybean cake powder, 4-6g fish meal, 2-4g KH
2PO
4, 1-2g MgSO
47H
2O, 0.1-0.5gMnSO
4H
2O, and 3-5gCaCO
3, original ph is 7.2-7.5.
The fermention medium of subtilis consists of to add in every premium on currency has 30-40g glucose, 4-6g (NH
4)
2SO
4, 6-10g wheat bran, 2-4g KH
2PO
4, 1-2.5g MgSO
47H
2O, 1-2.5g MnSO
4H
2O, 0.1-0.5g FeSO
47H
2O, and 3-5gCaCO
3, original ph is 7.2-7.5.
(3) adsorption dry: get CaCO by weight percentage
320-25%, and wheat bran 75-80% mixing as carrier (sorbent material); With above-mentioned each fermented liquid and carrier in 1: 1 ratio (i.e. 1 milliliter of fermented liquid: 1 gram carrier) adsorb respectively; Again respectively at 45-55 ℃ of oven dry; Drying time is 1.5-2.5h, is respectively 8-12% to the water cut of each single bacterium leavening agent, obtains each single bacterium leavening agent;
The temperature of above-mentioned oven drying at low temperature is 45-55 ℃, and the time is 1.5-2.5h, and the water cut of each single bacterium leavening agent of oven dry back is respectively 8-12%.
(4) get above-mentioned each single bacterium leavening agent by Bacillus coagulans 3-5 * 10
8CFU/g, side gemma spore bacillus 25-35 * 10
8CFU/g, bacillus natto 30-40 * 10
8CFU/g, and subtilis 25-35 * 10
8The mixed of CFU/g is even, obtains composite bacillus cotton dregs starter, and total count>=100 * 10 in this cotton dregs starter
8CFU/g.
The bacterial classification of using among the present invention is prior art, and preserving number is respectively: subtilis (Bacillus subtilis): ACCC11062, CGMCC2548 or CGMCC1222; Side gemma spore bacillus (Bacillus lateraporus): CGMCC1755 or CGMCC9701; Bacillus natto (Bacillus natto): CCTCC M207147, CGMCC 0724 or ATCC 6051; Bacillus coagulans (Bacillus coagulans): CICC20092, CGMCC1.2407 or ATCC7050.
Cotton dregs starter of the present invention is that the 0.8-1.5% by cotton dregs weight joins in the cotton dregs with the fermentation cotton dregs, removes free gossypol.
Compared with prior art, the present invention has the following advantages:
(1) bacterial classification that the inventive method adopted all is a genus bacillus, has strong stress resistance, is easy to characteristics such as preservation.The side gemma spore bacillus of adopting can produce antibacterial substance in process of growth, can suppress the growth of other assorted bacterium, and Bacillus coagulans can produce lactic acid simultaneously, can improve the palatability of cotton dregs;
(2) bacillus natto, Bacillus coagulans, side gemma spore bacillus and four kinds of mikrobes of subtilis of choosing of the inventive method; Utilize its comprehensive action; ANFs in the cotton dregs is eliminated from the content of gossypol in degradable cotton dregs middle reaches, improves proteinic utilization ratio; Have the effect of probiotics simultaneously concurrently, have effects such as the animal intestinal of improvement microecological balance, raising animal immunizing power;
(3) the present invention has that production technique is simple, energy consumption is low, the no three wastes, investment are little, be easy to characteristics such as large-scale production.
Embodiment:
Embodiment 1:
1, prepare each liquid spawn:
Liquid spawn culture medium (4 parts): adding in every premium on currency has glucose 30g, soybean cake powder 15g, (NH
4)
2SO
44g, KH
2PO
44g, MgSO
47H
2O 1.5g, NaCl5g, and CaCO
32g, initial pH7.2;
Get subtilis 1 strain inoculation one ring slant strains cell in a liquid spawn culture medium, at 37 ℃, under the 200r/min condition, shaking table shaking culture 60h makes the cell gemma rate of formation in the seed liquor reach 94.7%, 80 ℃ of water bath with thermostatic control processing 12min;
Get side gemma spore bacillus 1 strain inoculation one ring slant strains cell in liquid spawn culture medium, at 37 ℃, under the 200r/min condition, shaking table shaking culture 60h makes the cell gemma rate of formation in the seed liquor reach 92.6%, 80 ℃ of water bath with thermostatic control processing 12min;
Get bacillus natto 1 strain inoculation one ring slant strains cell in liquid spawn culture medium, at 37 ℃, under the 200r/min condition, shaking table shaking culture 60h makes the cell gemma rate of formation in the seed liquor reach 93%, 80 ℃ of water bath with thermostatic control processing 12min;
Get Bacillus coagulans 1 strain inoculation one ring slant strains cell in liquid spawn culture medium, at 37 ℃, under the 200r/min condition, shaking table shaking culture 60h makes the cell gemma rate of formation in the seed liquor reach 91.5%, 80 ℃ of water bath with thermostatic control processing 12min;
(2) fermentation:
In the Bacillus coagulans fermention medium, culture temperature is 40 ℃, inoculum size 5%, stirring velocity 180r/min, fermented incubation time 48h with above-mentioned Bacillus coagulans liquid-spawn inoculation,
The Bacillus coagulans fermention medium: adding in every premium on currency has wheat bran 15g, soybean cake powder 10g, steeping water 10g, KH
2PO
43g, MgSO
47H
2O 1.5g, MnSO
4H
2O 0.1g, and CaCO
34g, initial pH7.2;
Side gemma spore bacillus and bacillus natto liquid spawn are inoculated in respectively in side gemma spore bacillus fermentation substratum and the bacillus natto to ferment substratum, and culture temperature is 30 ℃, and inoculum size is 3%, stirring velocity 160r/min, fermentation time 72h;
Side gemma spore bacillus and bacillus natto to ferment substratum: adding in every premium on currency has glucose 30g, soybean cake powder 15g, fish meal 4g, KH
2PO
42.5g, MgSO
47H
2O 1g, MnSO
4H
2O 0.1g, and CaCO
34g, initial pH7.3;
With above-mentioned subtilis liquid-spawn inoculation in the fermentation of bacillus subtilis substratum, 30 ℃ of culture temperature, inoculum size 4%, stirring velocity 160r/min, fermented incubation time 72h,
The fermentation of bacillus subtilis substratum: adding in every premium on currency has glucose 30g, (NH
4)
2SO
46g, wheat bran 8g, KH
2PO
42.5g, MgSO
47H
2O 1.5, MnSO
4H
2O 1g, FeSO
47H
2O 0.1g, and CaCO
33g, initial pH7.2;
(3) adsorption dry: get 25%CaCO by weight percentage
3Make absorption carrier with 75% wheat bran mixing; Respectively with above-mentioned Bacillus coagulans fermented liquid, side gemma spore bacillus fermentation liquid, bacillus natto to ferment liquid, and fermentation of bacillus subtilis liquid and carrier in 1 milliliter: 1 ratio that restrains is adsorbed respectively; Respectively at 55 ℃ of dry 1.5h, obtain each single bacterium leavening agent again;
(4) cotton dregs starter preparation: is 4.5 * 10 with each dried single bacterium leavening agent by Bacillus coagulans
8CFU/g, side gemma spore bacillus are 32 * 10
8CFU/g, bacillus natto are 31.4 * 10
8CFU/g, and subtilis be 33.6 * 10
8The CFU/g mixed is even, and composite bacillus cotton dregs starter total count is 101.5 * 10
8CFU/g.
(5) the cotton dregs starter of the present invention that in cotton dregs, adds 1% weight is with the fermentation cotton dregs; Cotton dregs water cut 54%; Fermentation time 5d records free gossypol clearance 95.7% in the leavened prod after the oven dry, protein contnt improves 5.48%; Total free amino acid content improves 8.57%, and water cut is 10.3%.
Embodiment 2:
1, prepare each liquid spawn:
Liquid spawn culture medium (4 parts): adding in every premium on currency has glucose 35g, soybean cake powder 12g, (NH
4)
2SO
46g, KH
2PO
43g, MgSO
47H
2O 2.5g, NaCl3g, and CaCO
34g, initial pH7.5;
Get subtilis 1 strain inoculation one ring slant strains cell in a liquid spawn culture medium, at 40 ℃, under the 180r/min condition, shaking table shaking culture 48h makes the cell gemma rate of formation in the seed liquor reach 92.7%, 80 ℃ of water bath with thermostatic control processing 10min;
Get side gemma spore bacillus 1 strain inoculation one ring slant strains cell in liquid spawn culture medium, at 40 ℃, under the 180r/min condition, shaking table shaking culture 48h makes the cell gemma rate of formation in the seed liquor reach 91.3%, 80 ℃ of water bath with thermostatic control processing 10min;
Get bacillus natto 1 strain inoculation one ring slant strains cell in liquid spawn culture medium, at 40 ℃, under the 180r/min condition, shaking table shaking culture 48h makes the cell gemma rate of formation in the seed liquor reach 94.2%, 80 ℃ of water bath with thermostatic control processing 10min;
Get Bacillus coagulans 1 strain inoculation one ring slant strains cell in liquid spawn culture medium, at 40 ℃, under the 180r/min condition, shaking table shaking culture 48h makes the cell gemma rate of formation in the seed liquor reach 90.6%, 80 ℃ of water bath with thermostatic control processing 10min;
(2) fermentation:
In the Bacillus coagulans fermention medium, culture temperature is 30 ℃, inoculum size 6%, stirring velocity 160r/min, fermented incubation time 72h with above-mentioned Bacillus coagulans liquid-spawn inoculation,
The Bacillus coagulans fermention medium: adding in every premium on currency has wheat bran 25g, soybean cake powder 8g, steeping water 5g, KH
2PO
42g, MgSO
47H
2O 1g, MnSO
4H
2O 0.3g, and CaCO
33.5g, initial pH7.4;
Side gemma spore bacillus and bacillus natto liquid spawn are inoculated in respectively in side gemma spore bacillus fermentation substratum and the bacillus natto to ferment substratum, and culture temperature is 32 ℃, and inoculum size is 5%, stirring velocity 180r/min, fermentation time 60h;
Side gemma spore bacillus and bacillus natto to ferment substratum: adding in every premium on currency has glucose 25g, soybean cake powder 10g, fish meal 5g, KH
2PO
42g, MgSO
47H
2O 1.5g, MnSO
4H
2O 0.25g, and CaCO
33g, initial pH7.2;
With above-mentioned subtilis liquid-spawn inoculation in fermention medium, 32 ℃ of culture temperature, inoculum size 5%, stirring velocity 180r/min, fermented incubation time 64h,
The fermentation of bacillus subtilis substratum: adding in every premium on currency has glucose 35g, (NH
4)
2SO
44g, wheat bran 10g, KH
2PO
43g, MgSO
47H
2O 2.5g, MnSO
4H
2O1.5g, FeSO
47H
2O 0.3g, and CaCO
34g, initial pH7.5;
(3) adsorption dry: get 20%CaCO by weight percentage
3Make absorption carrier with 80% wheat bran mixing, with above-mentioned each fermented liquid and carrier by volume 1: 1 ratio of weight ratio (V/W) adsorb respectively, again respectively at 45 ℃ of dry 2.5h, obtain each single bacterium leavening agent;
(4) cotton dregs starter preparation: is 5 * 10 with each dried single bacterium leavening agent by Bacillus coagulans
8CFU/g, side gemma spore bacillus are 25.7 * 10
8CFU/g, bacillus natto are 36.8 * 10
8CFU/g, and subtilis be 34.6 * 10
8The CFU/g mixed is even, and composite bacillus cotton dregs starter total count is 102.1 * 10
8CFU/g.
(5) the cotton dregs starter of the present invention that in cotton dregs, adds 0.8% weight is with the fermentation cotton dregs; Cotton dregs water cut 48%; Fermentation time 5.5d records free gossypol clearance 94.61% in the leavened prod after the oven dry, protein contnt improves 6.13%; Total free amino acid content improves 9.21%, and water cut is 8.51%.
Embodiment 3:
1, prepare each liquid spawn:
Liquid spawn culture medium (4 parts): adding in every premium on currency has glucose 40g, soybean cake powder 10g, (NH
4)
2SO
42g, KH
2PO
42g, MgSO
47H
2O 1g, NaCl4g, and CaCO
33g, initial pH7.4;
Get subtilis, side gemma spore bacillus, bacillus natto, and each 1 strain of Bacillus coagulans inoculate a ring slant strains cell branch respectively in liquid spawn culture medium; At 39 ℃; Under the 160r/min condition, shaking table shaking culture 52h makes the cell gemma rate of formation in the seed liquor reach 95%; 15min is handled in 80 ℃ of waters bath with thermostatic control, makes each liquid spawn respectively;
(2) fermentation:
In the Bacillus coagulans fermention medium, culture temperature is 37 ℃, inoculum size 4%, stirring velocity 170r/min, fermented incubation time 50h with above-mentioned Bacillus coagulans liquid-spawn inoculation,
The Bacillus coagulans fermention medium: adding in every premium on currency has wheat bran 22g, soybean cake powder 7g, steeping water 6g, KH
2PO
44g, MgSO
47H
2O 2.5g, MnSO
4H
2O 0.2g, and CaCO
35g, initial pH7.3;
Side gemma spore bacillus and bacillus natto liquid spawn are inoculated in respectively in side gemma spore bacillus fermentation substratum and the bacillus natto to ferment substratum, and culture temperature is 40 ℃, and inoculum size is 6%, stirring velocity 170r/min, fermentation time 50h;
Side gemma spore bacillus and bacillus natto to ferment substratum: adding in every premium on currency has glucose 35g, soybean cake powder 18g, fish meal 5.5g, KH
2PO
43g, MgSO
47H
2O 2g, MnSO
4H
2O 0.3g, and CaCO
34.5g, initial pH7.4;
With above-mentioned subtilis liquid-spawn inoculation in fermention medium, 40 ℃ of culture temperature, inoculum size 6%, stirring velocity 170r/min, fermented incubation time 50h,
The fermentation of bacillus subtilis substratum: adding in every premium on currency has glucose 40g, (NH
4)
2SO
44g, wheat bran 6g, KH
2PO
42g, MgSO
47H
2O 1g, MnSO
4H
2O 2g, FeSO
47H
2O 0.1g, and CaCO
33.5g, initial pH7.3;
(3) adsorption dry: get 22%CaCO by weight percentage
3Make absorption carrier with 78% wheat bran mixing, with above-mentioned each fermented liquid and carrier by volume 1: 1 ratio of weight ratio (V/W) adsorb respectively, again respectively at 50 ℃ of dry 2h, obtain each single bacterium leavening agent;
(4) cotton dregs starter preparation: is 3 * 10 with each dried single bacterium leavening agent by Bacillus coagulans
8CFU/g, side gemma spore bacillus are 34.4 * 10
8CFU/g, bacillus natto are 39.8 * 10
8CFU/g, and subtilis be 27 * 10
8The CFU/g mixed is even, and composite bacillus cotton dregs starter total count is 104.2 * 10
8CFU/g.
(5) the cotton dregs starter of the present invention that in cotton dregs, adds 1.2% weight is with the fermentation cotton dregs; Cotton dregs water cut 51.3%; Fermentation time 6d records free gossypol clearance 96.27% in the leavened prod after the oven dry, protein contnt improves 5.92%; Total free amino acid content improves 9.03%, and water cut is 11.4%.
Claims (6)
1. a composite bacillus cotton dregs starter is characterized in that, contains Bacillus coagulans (Bacillus coagulans) 3-5 * 10 in the said starter
8CFU/g, side gemma spore bacillus (Bacillus lateraporus) 25-35 * 10
8CFU/g, bacillus natto (Bacillus natto) 30-40 * 10
8CFU/g, and subtilis (Bacillus subtilis) 25-35 * 10
8CFU/g, total count>=100 * 10
8CFU/g.
2. one kind prepares the method for composite bacillus cotton dregs starter according to claim 1, and it is characterized in that: this method comprises the steps:
(1) preparation liquid spawn: get subtilis, side gemma spore bacillus, bacillus natto, and each 1 strain of Bacillus coagulans inoculate a ring slant strains cell respectively in liquid spawn culture medium; At 37 ℃-40 ℃; Under the 160-200r/min condition, shaking table shaking culture 48-60h makes the cell gemma rate of formation in each seed liquor reach 90-95%; Handle 10-15min in 80 ℃ of waters bath with thermostatic control again, obtain four kinds of liquid spawns;
(2) fermentation: the inoculum size of above-mentioned each liquid spawn by 3-6% (V/V) is inoculated in respectively in the corresponding fermention medium,, cultivates 48-72h under the stirring velocity 160-180r/min condition at culture temperature 30-40 ℃;
(3) adsorption dry: get CaCO by weight percentage
320-25%, and wheat bran 75-80% mixing as carrier; With above-mentioned each fermented liquid and carrier respectively in 1mL: the ratio of 1g is adsorbed respectively, and again respectively at 45-55 ℃ of oven dry, drying time is 1.5-2.5h; Water cut to each single bacterium leavening agent is respectively 8-12%, obtains each single bacterium leavening agent;
(4) get above-mentioned each single bacterium leavening agent by Bacillus coagulans 3-5 * 10
8CFU/g, side gemma spore bacillus 25-35 * 10
8CFU/g, bacillus natto 30-40 * 10
8CFU/g, and subtilis 25-35 * 10
8The mixed of CFU/g is even, obtains composite bacillus cotton dregs starter, and total count>=100 * 10 in this cotton dregs starter
8CFU/g.
3. the preparation method of composite bacillus cotton dregs starter as claimed in claim 2 is characterized in that: the liquid spawn culture medium in the said step (1) consists of to add in every premium on currency has 30-40g glucose, 10-15g soybean cake powder, 2-6g (NH
4)
2SO
4, 2-4g KH
2PO
4, 1-2.5g MgSO
47H
2O, 3-5gNaCl, and 2-4gCaCO
3, original ph is 7.2-7.5.
4. the preparation method of composite bacillus cotton dregs starter as claimed in claim 2 is characterized in that: the fermention medium of said Bacillus coagulans consists of to add in every premium on currency has 15-25g wheat bran, 7-10g soybean cake powder, 5-10g steeping water, 2-4g KH
2PO
4, 1-2.5g MgSO
47H
2O, 0.1-0.3g MnSO
4H
2O, and 3-5gCaCO
3, original ph is 7.2-7.5.
5. the preparation method of composite bacillus cotton dregs starter as claimed in claim 2 is characterized in that: the fermention medium of said side gemma spore bacillus and bacillus natto consists of to add in every premium on currency has 25-35g glucose, 10-20g soybean cake powder, 4-6g fish meal, 2-4g KH
2PO
4, 1-2g MgSO
47H
2O, 0.1-0.5gMnSO
4H
2O, and 3-5gCaCO
3, original ph is 7.2-7.5.
6. the preparation method of composite bacillus cotton dregs starter as claimed in claim 2 is characterized in that: the fermention medium of said subtilis consists of to add in every premium on currency has 30-40g glucose, 4-6g (NH
4)
2SO
4, 6-10g wheat bran, 2-4g KH
2PO
4, 1-2.5g MgSO
47H
2O, 1-2.5g MnSO
4H
2O, 0.1-0.5g FeSO
47H
2O, and 3-5gCaCO
3, original ph is 7.2-7.5.
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| CN102924181A (en) * | 2012-11-22 | 2013-02-13 | 塔城市星河生物工程有限责任公司 | Vegetable amino acid liquid fertilizer and preparing method |
| CN104605139A (en) * | 2015-01-08 | 2015-05-13 | 河北农业大学 | Bacillus subtilis-acanthopanax senticosus polysaccharide synbiotics, preparation method thereof and application of bacillus subtilis-acanthopanax senticosus polysaccharide synbiotics as feed additive |
| CN105685490B (en) * | 2016-01-26 | 2020-06-30 | 曲阜师范大学 | Stevia rebaudiana waste residue fermentation product compound detoxified cottonseed kernel feed and preparation method thereof |
| CN106071106B (en) * | 2016-06-17 | 2019-12-13 | 四川伯禹生物工程有限公司 | Fermented cottonseed protein and method for preparing fermented cottonseed protein by solid-state aerobic fermentation |
| CN107683954B (en) * | 2017-09-17 | 2018-07-31 | 浙江大学 | An a kind of step adds the method that bacterium step fermentation prepares low anti-nutritional factors acidifying feed |
| CN113604404B (en) * | 2021-08-31 | 2022-08-16 | 四川润格生物科技有限公司 | Bacillus coagulans YSF17 and application thereof |
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| CN101444252A (en) * | 2008-11-18 | 2009-06-03 | 广东省农业科学院农业生物技术研究所 | Method for preparing fermented virus-free cottonseed meal feed protein |
| CN101810244A (en) * | 2010-04-29 | 2010-08-25 | 湖南农业大学 | Biological detoxification method of cottonseed meal |
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| CN101444252A (en) * | 2008-11-18 | 2009-06-03 | 广东省农业科学院农业生物技术研究所 | Method for preparing fermented virus-free cottonseed meal feed protein |
| CN101810244A (en) * | 2010-04-29 | 2010-08-25 | 湖南农业大学 | Biological detoxification method of cottonseed meal |
Non-Patent Citations (1)
| Title |
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| 叶明强等.固体发酵棉籽粕菌种组合的筛选.《中国饲料》.2009,(第15期),8-10,14. * |
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