CN107683954B - An a kind of step adds the method that bacterium step fermentation prepares low anti-nutritional factors acidifying feed - Google Patents

An a kind of step adds the method that bacterium step fermentation prepares low anti-nutritional factors acidifying feed Download PDF

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CN107683954B
CN107683954B CN201710836699.9A CN201710836699A CN107683954B CN 107683954 B CN107683954 B CN 107683954B CN 201710836699 A CN201710836699 A CN 201710836699A CN 107683954 B CN107683954 B CN 107683954B
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fermentation
zjuaf
seed liquor
hours
feedstuff
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CN107683954A (en
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汪以真
王成
路则庆
王凤芹
张煜
石常友
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Zhejiang University ZJU
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/26—Compounds containing phosphorus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The invention discloses an a kind of steps to add the method that bacterium step fermentation prepares low anti-nutritional factors acidifying feed, and feedstuff and the mass ratio of second order fermentation seed liquor are pressed 2:1 stirs and evenly mixs, and anaerobic fermentation is carried out after aerobic fermentation 18 ~ 20 hours 40 ~ 46 hours to get to finished product；The preparation of second order fermentation seed liquor：The bacillus subtilis ZJUAF 3 of fresh cultured and bacillus coagulans ZJUAF 4 are inoculated with respectively in 5mL LB liquid mediums, cultivates 4 ~ 6 hours, makes OD600Reach 0.5, obtain 4 one grade fermemtation seed liquor of 3 primary seed solutions of ZJUAF and ZJUAF, one grade fermemtation seed liquor is separately added into 37 DEG C of cultures in feedstuff leaching juice with whole quality 2% ~ 3% and obtains second order fermentation seed liquor in 8 hours.The present invention utilizes the bacterial strain of efficient compatibility, improves production technology, connects bacterium stirring fermentation by a step, greatly improves production efficiency, and fermentation verification obtains good result in different feeds.The fermented feed product resultant effect of production is good, while being suitble to siccative and wet feed sale, has good production application foreground.

Description

An a kind of step adds the method that bacterium step fermentation prepares low anti-nutritional factors acidifying feed
Technical field
The present invention relates to feed, microbial fermentation biotechnologies, and in particular to synchronize connect bacterium step fermentation prepare it is low The method of anti-nutritional factors acidifying feed.
Background technology
Food security and problems of ecological security were more and more of interest by society of various countries and government in recent years concerning the people's livelihood matter of fundamental importance.It supports The final goal for growing industry is exactly the animal products such as the meat, eggs and milk of the high-quality green of production safety, is promoted the well-being of mankind.Feedstuff industry is as foster The upstream industry for growing industry plays an important role in entire industrial chain.Antibiotic in aquaculture apply the past very Long a period of time has pushed the development of aquaculture, but abuse of antibiotics is reported again and again in recent years, and remaining antibiotic passes through Food chain reaches human body, can seriously damage the health of people, cause the generation of drug tolerant bacteria, while polluting environment.1986 auspicious Allusion quotation takes the lead in forbidding the use of growth promotion antibiotic, and European Union completely forbids the use of growth promotion antibiotic, the U.S. in 2014 within 2006 It announces gradually to forbid the use for promoting to grow antibiotic at following 3 years, on April 1st, 2017, China announced to forbid sulfuric acid viscous Bacillin addition is used as growth promoter in feed.The useless dirty inappropriate discharge of farm's exhaust gas simultaneously, affects ecological ring Border, government increasingly advocate green cultivation.Therefore safety is urgently developed for anti-and green high-efficient novel fodder.
China is populous nation, and cultivation big country.85,400,000 tons of China's meat total output in 2016, pork yield 5299 Ten thousand tons；7,170,000 tons of beef production, 4,590,000 tons of Mutton yield, 18,880,000 tons of poultry yield, 30,950,000 tons of birds, beasts and eggs yield, former milk production Measure 38,000,000 tons.2.9 hundred million tons of China's commercial feed yield in 2016.
Wean be to young animal it is huge stress, the early weaned technology of scale livestock farming is even more that exacerbate stress journey Degree.Young animal growth metabolism is vigorous, but intestinal growth is unsound, and gastric acid secretion is insufficient, easily causes to digest to protide feed Bad, for the bad product of generation to causing intestinal mucosa to stimulate, intestinal villus is impaired, especially easy to vegetable protein and bad oligosaccharides Allergic reaction is generated, oxidative stress is produced, and high molecular weight protein and non-starch polysaccharide etc. are easy to cause young animal indigestion, Eventually lead to diarrhea.Female livestock breed performance is directly related to the economic benefit of farm, and the dam gestational period does not control nutrition Intake is easy to influence its lactation period output of milk, product subnumber and litter weight.Therefore, trophic factors, easy to digest is resisted in exploitation, and palatability is good Cub and dam feed rich in probiotic lactic acid and small peptide are of great significance.
Improve feed nutritive value by the means of microbial fermentation, produce green high-efficient feed have become at present it is foster Grow the hot spot that industry is studied under " nonreactive " background.Fermentative feedstuff of microbe can not only provide beneficial bacterium and its metabolite, adjust Gastrointestinal tract pH safeguards that intestinal flora balances, reduces the use of antibiotic, improves nutrient digestibility, moreover it is possible to develop and use new feeding Expect resource, alleviates Food Security.The lactic acid that probiotics generates simultaneously can also inhibit the pathogenic bacterias such as Escherichia coli in feed With the growth and breeding of pathogen, extend the feed holding time.The fermented feed principal mode of solid-state production at present has aerobic fermentation And anaerobic fermentation, aerobic fermentation are conducive to anti-nutritional factors and macro-nutrients in degradation feed, improve animal digestion and inhale Receipts ability；Anaerobic fermentation is then conducive to generate bionic acid, inhibits living contaminants, adjusts animal gastrointestinal tract pH, and it is agreeable to the taste to improve feed Property.The sale form of fermented feed mainly has siccative sale and wet feed sale.Siccative sale is not easy secondary microbiological contamination corruption, but sets Standby input is big, and drying course is easy to kill probiotics and its metabolite of poor heat resistance；Wet feed is sold：Equipment investment is small, makes Probiotics and its metabolite are not inactivated, but it is rotten to be easy to cause secondary microbiological contamination.
Wang is really to wait in " bacillus of efficient degradation dregs of beans antigen protein and the method for bacterium enzyme mixed fermentation "（The patent No. ZL201610728880.3）Describe the method for preparing low anti-nutritional factors acidification mixed feed in detail, but above-mentioned patent system is standby Method has the following disadvantages：
1. since bacillus subtilis is aerobic bacteria, and lactic acid bacteria is anaerobic bacteria, the former wants air agitation in culture, Inoculum density is can reach within general 6 hours, and the latter needs deep drainpipe, and incubation time needs 12 hours.In addition, two class bacterium Culture medium it is also different, the former provides nitrogen source and inorganic salts, and the latter requires supplementation with carbon source.Prepare before being fermented When, it needs to prepare 3 groups of culture tanks, it is cumbersome.
2. since two class bacterium are to oxygen demand difference, to achieve the purpose that fully degraded antigen protein, which is divided Step plus bacterium, step fermentation.But the equipment of middle-size and small-size Feed Enterprise is generally difficult to support substep plus bacterium, increases production cost.
3. the lactic acid bacteria used in the patented method is not easy to survive in feed drying processing, it is unfavorable for feeding subsequent supervention Wave the probiotic effects of bacterial strain itself.
Invention content
For overcome the deficiencies in the prior art, the present invention provides one kind one step add bacterium step fermentation prepare low anti-nutrition because The method of sub- acidifying feed.
An a kind of step adds the method that bacterium step fermentation prepares low anti-nutritional factors acidifying feed, and feedstuff and two level are sent out The mass ratio of ferment seed liquor presses 2:1 stirs and evenly mixs, anaerobic fermentation is carried out after aerobic fermentation 18 ~ 20 hours 40 ~ 46 hours to get To finished product；The preparation of the second order fermentation seed liquor：By the bacillus subtilis ZJUAF-3 and bacillus coagulans of fresh cultured ZJUAF-4 is inoculated with respectively in 5mL LB liquid mediums, is cultivated 4 ~ 6 hours, is made OD600Reach 0.5, obtains ZJUAF-3 level-ones Seed liquor and ZJUAF-4 one grade fermemtation seed liquors, one grade fermemtation seed liquor are separately added into feedstuff with whole quality 2% ~ 3% and soak juice It is cultivated 8 hours for 37 DEG C in liquid, so that OD600 is reached 0.5, obtain ZJUAF-3 second order fermentations seed liquor and ZJUAF-4 second order fermentation kinds Sub- liquid.
Bacillus subtilis ZJUAF-3 and bacillus coagulans ZJUAF-4 be all existing bacterium, wherein ZJUAF-3 for the first time It is disclosed in CN106167782 A（CN201610728303.4）, it is common to be preserved in China Committee for Culture Collection of Microorganisms Microorganism center, deposit number are CGMCC NO：12825, bacillus coagulans ZJUAF-4 are from Chinese Microbiological Culture Collection pipe The common micro-organisms center purchase of the reason committee, preserving number are CGMCC NO：1.2009, there is preferable lactic acid producing ability.
The ZJUAF-3 second order fermentations seed liquor and ZJUAF-4 second order fermentation seed liquors, by volume 1:1~1:1.5 Ratio be added in feedstuff and stir and evenly mix.
The preparation of the feedstuff leaching juice：By mass percentage, 6% feedstuff powder, 0.8% Na2HPO4· 12H2O, 0.4% (NH4)2SO4, 0.15% NH4Cl is added water and stirs 10min, after the completion of preparation, 90 DEG C of sterilizing 30min.
The feedstuff is corn, dregs of beans, wheat bran and corn DDGS is one or more and/or above-mentioned raw materials are puffing It is one or more in product.
30 ~ 40 DEG C of the fermentation temperature.
Beneficial effects of the present invention：
It is fermented using the bacterial strain compatibility of the present invention, the production procedure that original multistep can be connect the stirring of bacterium multistep is reduced to one Step plus bacterium stirring, substantially increase production efficiency, reduce production cost.And in single raw material（Dregs of beans）, mixed fodder（It is beautiful Rice-dregs of beans）With low value vinasse raw material（DDGS）On demonstrate the effect of the method, it was demonstrated that the practicability of the method.Feed is former Material can pack transport after the aerobic fermentation of short period, need not additionally add enzyme preparation, reduce production cost, generate A large amount of lactic acid inhibit harmful varied bacteria growing, alleviate feed conveying and preserve in living contaminants the problem of.
Two plants of bacterium compatibilities are good, and resistance is strong, can resist the harsh environments such as high temperature drying, smoothly reach animal intestinal tract Play prebiotic effect.Find, fermented feed produced by the invention, golden yellow color that there is sour fragrance, anti-nutritional factors drop after testing Inefficient fruit is good, is rich in prebiotic bacterium number, and ferment small peptide, lactic acid and other somatomedins, can reduce young animal diarrhea, improves female Reproductive performance is raiseeed, and at the same time being suitble to siccative and wet feed sale, is had a good application prospect.
Description of the drawings
Fig. 1 is the method for the present invention（Left half）And ZL201610728880.3（Right half）Fermented feed production technology stream Journey comparison diagram；
Fig. 2 is present invention process fermentation different feeds raw material anti-nutritional factors degradation effect electrophoretogram；
Fig. 3 is compatibility bacterial strain of the present invention compatibility strain fermentation forage protein degradation effect electrophoretogram different from other；
Fig. 4 is the lactic acid and pH variation diagrams of the fermented maize dregs of beans mixed fodder produced using the method for the present invention；
Fig. 5 is the micro organism quantity variation diagram of the fermented maize dregs of beans mixed fodder produced using the method for the present invention；
Fig. 6 is the fermented feed color and luster produced using the method for the present invention.
Specific implementation mode
Add bacterium step fermentation to prepare low anti-nutritional factors acidification one step of the present invention below by way of embodiments and drawings to raise
The method of material is further elaborated.
As shown in Figure 1, the fermented feed technological process of production that the present invention improves patent will add bacterium stirring step by step twice before Production technology be reduced to a step bacterium added to stir, substantially increase production efficiency, reduce production cost；
As shown in Figure 2, the fermented feed technological process of production provided by the invention, there is good fermentation in different feeds Effect demonstrates the practicability and versatility of this method.
Embodiment 1
One, formula feed raw material soaks juice（Activation medium）：By mass percentage, 4% bean cake powder, 3% corn flour, 0.8% Na2HPO4·12H2O, 0.4% (NH4)2SO4, 0.15% NH4Cl is added water and stirs 10min, after the completion of preparation, filters off insoluble matter Matter, 90 DEG C sterilizing 30min after cooled to room temperature.
Two, second order fermentation seed liquor is prepared：By the bacillus subtilis ZJUAF-3 of fresh cultured and condensation gemma bar ZJUAF-4 is inoculated with respectively in 5 mL LB liquid mediums（There are two pipes, each bacterium 5 mL mono- pipe）, cultivate 4 ~ 6 hours, make OD600Reach 0.5, obtains ZJUAF-3 primary seed solutions and ZJUAF-4 primary seed solutions, the primary seed solution after being cultivated by 1mL It is separately added into 37 DEG C of amplifications in feedstuff leaching juice with whole quality 2% ~ 3% to cultivate 8 hours, makes OD600Reach 0.5, obtains respectively Secondary seed solution after it is spare.
Three, fermented maize bean pulp type mixed fodder：200Kg corns, 200Kg dregs of beans are mixed, 100 kilograms of wheat bran conducts are added Auxiliary material is made into mixed fodder, and 250 kilograms of second order fermentation seed liquor, the secondary seed solution of wherein ZJUAF-3 and ZJUAF-4 is added By 1:1.5 ratio addition, it is about the 40% of total fermented quality to make water content（Feedstuff contains about 10% moisture）, it is mixed After uniformly, fermentation mixed fodder 1 is obtained within 24 hours in room temperature aerobic fermentation.Then fermentation mixed fodder 1 is packaged in anaerobism hair In ferment device.Fermentation obtains finished product after 48 hours.
To 1 fermented bean dregs of embodiment carry out nutritional ingredient detection, and with existing patented method, certain commercial strain fermentation side Method effect compares.As a result as described in Table 1：
As shown in Table 1 the embodiment of the present invention 1 method fermentation mixed fodder feed antigen protein degradation, small peptide improve, PH, prebiotic bacterium number etc. effect are better than ZL201610728880.3, and are substantially better than certain commercial strain ferment effect.
As shown in figure 3, the bacterial strain compatibility used in the present invention is better than the ferment effect of feed the hair with other bacterial strain compatibilities Ferment corn bean pulp type mixed fodder effect, illustrates the well-formedness and high efficiency of bacterial strain uses therefor compatibility of the present invention.
Embodiment 2
One, formula feed raw material soaks juice（Activation medium）：By mass percentage, 5% bean cake powder, 0.8% Na2HPO4· 12H2O, 0.4% (NH4)2SO4, 0.15% NH4Cl is added water and stirs 10min, after the completion of preparation, culture medium is heated to 90 DEG C Cooled to room temperature afterwards.
Two, second order fermentation seed liquor liquid is prepared：By the bacillus subtilis ZJUAF-3 of fresh cultured and condensation gemma bar ZJUAF-4 is inoculated with respectively in two pipes, 5 mL LB liquid mediums, cultivates 4 ~ 6 hours, OD600 is made to reach 0.5, is cultivated by 1mL Primary seed solution afterwards is separately added into 37 DEG C of amplifications in feedstuff leaching juice with whole quality 2% ~ 3% and cultivates 8 hours, makes OD600It reaches To 0.5, obtain spare after respective secondary seed solution.
Three, fermented bean dregs：Take 500Kg dregs of beans, be added 250 kilograms of second order fermentation seed liquor, wherein ZJUAF-3 and The secondary seed solution of ZJUAF-4 presses 1:1 ratio addition, it is the 40% of total fermented quality to make water content, after being mixed evenly, Fermented bean dregs 1 are obtained in room temperature aerobic fermentation within 24 hours, then will obtain finished product after 1 anaerobic fermentation of fermented bean dregs 48 hours.
To 2 fermented bean dregs of embodiment carry out nutritional ingredient detection, and with existing patented method, certain commercial strain fermentation side Method effect compares.As a result as described in Table 2：
The method fermented bean dregs of the embodiment of the present invention 2 are in the degradation of feed antigen protein, small peptide raising, pH, benefit as shown in Table 2 The ferment effect with obvious effects for being better than ZL201610728880.3 technical solutions above aerobic plate count.
As shown in Figure 4, dregs of beans pH is gradually reduced after present invention fermentation, and lactic acid content gradually accumulates, sour fragrance.
Escherichia coli Growth is suppressed after the completion of illustrating fermented bean dregs such as Fig. 5, is less than 1.0 × 103CFU/g, is conducive to Feed preserves, and for flora based on bacillus subtilis and bacillus coagulans, prebiotic effect is strong in feed.
Embodiment 3
One, formula feed raw material soaks juice（Activation medium）：By mass percentage, 5% corn DDGS powder, 0.8% Na2HPO4·12H2O, 0.4% (NH4)2SO4, 0.15% NH4Cl is added water and stirs 10min, after the completion of preparation, by culture medium plus Heat is to cooled to room temperature after 90 DEG C.
Two, second order fermentation seed liquor is prepared：By the bacillus subtilis ZJUAF-3 of fresh cultured and condensation gemma bar ZJUAF-4 is inoculated with respectively in two pipes, 5 mL LB liquid mediums, cultivates 4 ~ 6 hours, OD600 is made to reach 0.5, is cultivated by 1mL Primary seed solution afterwards is separately added into 37 DEG C of amplifications in feedstuff leaching juice with whole quality 2% ~ 3% and cultivates 8 hours, makes OD600It reaches To 0.5, obtain spare after respective secondary seed solution.
Three, fermented maize DDGS:500Kg corn DDGSs are taken, 250 kilograms of second order fermentation seed liquor is added, wherein The secondary seed solution of ZJUAF-3 and ZJUAF-4 presses 1:1 ratio addition, it is the 40% of total fermented quality to make water content, and mixing is stirred After mixing uniformly, fermentation DDGS1 is obtained within 24 hours in room temperature aerobic fermentation, is then after 48 hours by fermentation DDGS1 anaerobic fermentations Obtain finished product.
Nutritional ingredient detection is carried out to 3 fermented maize DDGS of embodiment, and is carried out with certain commercial strain fermentation process effect Compare.As a result as described in Table 3：
The method fermented maize DDGS effects of the embodiment of the present invention 3 are better than certain commercial strain ferment effect as shown in Table 3.
Fig. 6 shows in fermented feed provided by the invention that for fermented product in golden yellow, business sense organ value is high.

Claims (1)

1. an a kind of step adds the method that bacterium step fermentation prepares low anti-nutritional factors acidifying feed, which is characterized in that by feed original Material and the mass ratio of second order fermentation seed liquor press 2:1 stirs and evenly mixs, carried out after aerobic fermentation 18 ~ 20 hours anaerobic fermentation 40 ~ 46 hours to get to finished product；
The preparation of the second order fermentation seed liquor：By the bacillus subtilis ZJUAF-3 and bacillus coagulans of fresh cultured ZJUAF-4 is inoculated with respectively in 5mL LB liquid mediums, is cultivated 4 ~ 6 hours, is made OD600Reach 0.5, obtains ZJUAF-3 level-ones Seed liquor and ZJUAF-4 one grade fermemtation seed liquors, one grade fermemtation seed liquor are separately added into feedstuff with whole quality 2% ~ 3% and soak juice It is cultivated 8 hours for 37 DEG C in liquid, makes OD600Reach 0.5, obtains ZJUAF-3 second order fermentations seed liquor and ZJUAF-4 second order fermentation kinds Sub- liquid；
The ZJUAF-3 second order fermentations seed liquor and ZJUAF-4 second order fermentation seed liquors, by volume 1:1~1:1.5 ratio Example, which is added in feedstuff, to be stirred and evenly mixed；
The preparation of the feedstuff leaching juice：By mass percentage, 6% feedstuff powder, 0.8% Na2HPO4·12H2O, 0.4% (NH4)2SO4, 0.15% NH4Cl is added water and stirs 10min, after the completion of preparation, 90 DEG C of sterilizing 30min；
The feedstuff is corn, dregs of beans, wheat bran and corn DDGS is one or more and/or above-mentioned raw materials dilated product In it is one or more；
30 ~ 40 DEG C of the fermentation temperature.
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CN1570082A (en) * 2004-04-27 2005-01-26 包头市天赋食品有限公司 Multiple strain microorganism ferment production method and its uses in cow fine fodder
CN102008006A (en) * 2009-09-08 2011-04-13 中国农业科学院饲料研究所 Preparation method of polypeptide for fermented feed with molecular weight of less than 15KDa
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