CN108041348A - A kind of preparation method of sea cucumber solid fermentation bait - Google Patents
A kind of preparation method of sea cucumber solid fermentation bait Download PDFInfo
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- CN108041348A CN108041348A CN201711327396.0A CN201711327396A CN108041348A CN 108041348 A CN108041348 A CN 108041348A CN 201711327396 A CN201711327396 A CN 201711327396A CN 108041348 A CN108041348 A CN 108041348A
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- sea cucumber
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- lactic acid
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- 238000000855 fermentation Methods 0.000 title claims abstract description 25
- 230000004151 fermentation Effects 0.000 title claims abstract description 25
- 241000251511 Holothuroidea Species 0.000 title claims abstract description 17
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 239000007787 solid Substances 0.000 title claims abstract description 12
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 30
- 241000894006 Bacteria Species 0.000 claims abstract description 15
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 15
- 239000004310 lactic acid Substances 0.000 claims abstract description 15
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 14
- 239000000843 powder Substances 0.000 claims abstract description 12
- 108091005508 Acid proteases Proteins 0.000 claims abstract description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 9
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 9
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 7
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 241000512259 Ascophyllum nodosum Species 0.000 claims abstract description 5
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 5
- 230000001580 bacterial effect Effects 0.000 claims abstract description 5
- 239000004467 fishmeal Substances 0.000 claims abstract description 5
- 239000012530 fluid Substances 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims abstract description 5
- 239000000203 mixture Substances 0.000 claims abstract description 5
- 238000004806 packaging method and process Methods 0.000 claims abstract description 5
- 241000195474 Sargassum Species 0.000 claims abstract description 4
- 235000012054 meals Nutrition 0.000 claims abstract description 3
- 241000238557 Decapoda Species 0.000 claims description 4
- 241000194108 Bacillus licheniformis Species 0.000 claims description 3
- 235000013351 cheese Nutrition 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 2
- 230000004083 survival effect Effects 0.000 abstract description 2
- 241000143060 Americamysis bahia Species 0.000 abstract 1
- 241000965254 Apostichopus japonicus Species 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Marine Sciences & Fisheries (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of sea cucumber preparation methods of solid fermentation bait, comprise the following steps：1. by zymophyte, water, is added in container and is uniformly mixed, standing for fermentation activates bacterial strain, and zymotic fluid is made；The zymophyte is made of according to mass fraction bacillus 45~60%, lactic acid bacteria 23~30%, saccharomyces cerevisiae 17~25%；2. is by fermented bean dregs；Fish meal；Shrimp med；Sargassum powder；Soy meal；Degumming Kelp Powder；Acid protease is uniformly mixed；3. 1. zymocyte liquid for preparing step is added in 2. mixture that step is prepared, it is uniformly mixed；It is sealed by fermentation 4. 3. mixed feed that obtains step is put into packaging bag；25~30 DEG C ferment 68 days.Simple production process of the present invention is easily operated, extensive easy to spread, the with short production cycle, good product quality at low cost of raw material sources, easy to spread and application.It is applied in holothruian cultures, sea cucumber survival rate and growth performance can be improved.
Description
Technical field
The present invention relates to a kind of sea cucumber preparation methods of solid fermentation bait.
Background technology
In recent years, apostichopus japonicus culture industry is grown rapidly in the coastal various regions of northern China, has huge market potential, and gradually
One of important industry as China's sea-farming achieves significant economic benefit and social benefit.In traditional breeding process
In, often added in feed anti-to reduce the carrying amount of microorganism or the effect of raising disease treatment, raiser in feed
Raw element, the use of a large amount of antibiotic not only make immunity degradation, the induction pathogenic bacteria generation drug resistance of stichopus japonicus in itself, but also band
Carry out the serious problems such as medicament residue, jeopardize food security and human health.
The content of the invention
To solve the above-mentioned problems, the present invention provides a kind of preparation method of sea cucumber solid fermentation bait.
The invention is realized by the following technical scheme：
A kind of sea cucumber preparation method of solid fermentation bait, comprises the following steps：
1. by 25-35 parts of zymophyte, 280~360 parts of water, is added in container and is uniformly mixed, stand according to parts by weight
Fermentation 60~80 minutes, fermentation temperature are 32~35 DEG C, activate bacterial strain, and zymotic fluid is made；The zymophyte is according to quality point
Number is made of bacillus 45~60%, lactic acid bacteria 23~30%, saccharomyces cerevisiae 17~25%；
2. is in parts by weight, by 260~350 parts of fermented bean dregs；100~120 parts of fish meal；40~60 parts of shrimp med；Horse
20~40 parts of tail algae powder；60~100 parts of soy meal；80~120 parts of degumming Kelp Powder；9~10 parts of acid protease is uniformly mixed；
3. 1. zymocyte liquid for preparing step is added in 2. mixture that step is prepared, it is uniformly mixed；
It is sealed by fermentation 4. 3. mixed feed that obtains step is put into packaging bag；25~30 DEG C ferment 6-8 days.
Preferably, the bacillus is selected from bacillus subtilis and/or bacillus licheniformis.
Preferably, bacillus 2 × 107~2 × 108CFU/g, lactic acid bacteria 1 × 107~1 × 108CFU/g, saccharomyces cerevisiae 1
×107~1 × 108CFU/g；Acid protease 1 × 108~1 × 109IU/g。
Preferably, step 1. described in lactic acid bacteria be selected from cheese lactic acid bacteria.
Invention advantageous effect
The present invention improves the digestive utilization ratio of sea cucumber bait and the growth performance of sea cucumber using microbial fermentation technology；
Simple production process of the present invention is easily operated, and raw material sources are extensive, at low cost easy to spread, with short production cycle, product matter
It measures, be easy to apply and promote；After the product is applied in holothruian cultures, sea cucumber survival rate and growth performance can be improved, can be changed
The ecological environment of kind aquaculture pond water body purifies water, and is a kind of preferable, high-quality microbial fermentation bait for sea cucumbers.
Specific embodiment
Following non-limiting examples can make those of ordinary skill in the art be more fully understood the present invention, but not with
Any mode limits the present invention.
Embodiment 1
A kind of sea cucumber preparation method of solid fermentation bait, comprises the following steps：
1. by 25 parts of zymophyte, 280 parts of water, adds in container and is uniformly mixed, be left to ferment 60 points according to parts by weight
Clock, fermentation temperature are 32~35 DEG C, activate bacterial strain, and zymotic fluid is made；The zymophyte is according to mass fraction by bacillus
45%th, lactic acid bacteria 30%, saccharomyces cerevisiae 25% forms；
2. is in parts by weight, by 260 parts of fermented bean dregs；100 parts of fish meal；40 parts of shrimp med；20 parts of sargassum powder；Greatly
60 parts of bean powder；80 parts of degumming Kelp Powder；9 parts of acid protease is uniformly mixed；
3. 1. zymocyte liquid for preparing step is added in 2. mixture that step is prepared, it is uniformly mixed；
It is sealed by fermentation 4. 3. mixed feed that obtains step is put into packaging bag；25~30 DEG C ferment 6-8 days.
Bacillus is selected from bacillus subtilis.
Bacillus 2 × 107~2 × 108CFU/g, lactic acid bacteria 1 × 107~1 × 108CFU/g, saccharomyces cerevisiae 1 × 107~1
×108CFU/g；Acid protease 1 × 108~1 × 109IU/g。
Embodiment 2
A kind of sea cucumber preparation method of solid fermentation bait, comprises the following steps：
1. by 35 parts of zymophyte, 360 parts of water, adds in container and is uniformly mixed, be left to ferment 80 points according to parts by weight
Clock, fermentation temperature are 32~35 DEG C, activate bacterial strain, and zymotic fluid is made；The zymophyte is according to mass fraction by bacillus
60%th, lactic acid bacteria 23%, saccharomyces cerevisiae 17% forms；
2. is in parts by weight, by 350 parts of fermented bean dregs；120 parts of fish meal；60 parts of shrimp med；40 parts of sargassum powder；Greatly
100 parts of bean powder；20 parts of degumming Kelp Powder；10 parts of acid protease is uniformly mixed；
3. 1. zymocyte liquid for preparing step is added in 2. mixture that step is prepared, it is uniformly mixed；
It is sealed by fermentation 4. 3. mixed feed that obtains step is put into packaging bag；25~30 DEG C ferment 6-8 days.
Bacillus is selected from bacillus licheniformis.
Preferably, bacillus 2 × 107~2 × 108CFU/g, lactic acid bacteria 1 × 107~1 × 108CFU/g, saccharomyces cerevisiae 1
×107~1 × 108CFU/g；Acid protease 1 × 108~1 × 109IU/g。
The lactic acid bacteria is selected from cheese lactic acid bacteria.
Claims (4)
1. a kind of sea cucumber preparation method of solid fermentation bait, it is characterised in that comprise the following steps：
1. by 25~35 parts of zymophyte, 280~360 parts of water, is added in container and is uniformly mixed, be left to ferment according to parts by weight
60~80 minutes, fermentation temperature was 32~35 DEG C, activates bacterial strain, and zymotic fluid is made；The zymophyte according to mass fraction by
Bacillus 45~60%, lactic acid bacteria 23~30%, saccharomyces cerevisiae 17~25% form；
2. is in parts by weight, by 260~350 parts of fermented bean dregs；100~120 parts of fish meal；40~60 parts of shrimp med；Sargassum
20~40 parts of powder；60~100 parts of soy meal；80~120 parts of degumming Kelp Powder；9~10 parts of acid protease is uniformly mixed；
3. 1. zymocyte liquid for preparing step is added in 2. mixture that step is prepared, it is uniformly mixed；
It is sealed by fermentation 4. 3. mixed feed that obtains step is put into packaging bag；25~30 DEG C ferment 6-8 days.
2. according to the claim 1 sea cucumber preparation method of solid fermentation bait, it is characterised in that：The bacillus is selected from withered
Careless bacillus and/or bacillus licheniformis.
3. according to the claim 1 sea cucumber preparation method of solid fermentation bait, it is characterised in that：Bacillus 2 × 107~2 ×
108CFU/g, lactic acid bacteria 1 × 107~1 × 108CFU/g, saccharomyces cerevisiae 1 × 107~1 × 108CFU/g；Acid protease 1 × 108
~1 × 109IU/g。
4. according to the claim 1 sea cucumber preparation method of solid fermentation bait, it is characterised in that：Step 1. described in lactic acid bacteria
Selected from cheese lactic acid bacteria.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711327396.0A CN108041348A (en) | 2017-12-13 | 2017-12-13 | A kind of preparation method of sea cucumber solid fermentation bait |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711327396.0A CN108041348A (en) | 2017-12-13 | 2017-12-13 | A kind of preparation method of sea cucumber solid fermentation bait |
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| Publication Number | Publication Date |
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| CN108041348A true CN108041348A (en) | 2018-05-18 |
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| CN201711327396.0A Pending CN108041348A (en) | 2017-12-13 | 2017-12-13 | A kind of preparation method of sea cucumber solid fermentation bait |
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| Country | Link |
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| CN (1) | CN108041348A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113974024A (en) * | 2021-11-01 | 2022-01-28 | 中国热带农业科学院热带生物技术研究所 | Sargassum liquid fermented feed additive and preparation method thereof and application in aquaculture |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102258146A (en) * | 2011-08-19 | 2011-11-30 | 大连理工大学 | Sea cucumber microorganism fermented feed and preparation method thereof |
| KR20140026985A (en) * | 2012-08-26 | 2014-03-06 | 전라남도 | Formulated feeds for sea cucumber |
| CN105211550A (en) * | 2015-10-16 | 2016-01-06 | 大连工业大学 | A kind of preparation method of mixed culture solid state fermentation sea cucumber bait |
-
2017
- 2017-12-13 CN CN201711327396.0A patent/CN108041348A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102258146A (en) * | 2011-08-19 | 2011-11-30 | 大连理工大学 | Sea cucumber microorganism fermented feed and preparation method thereof |
| KR20140026985A (en) * | 2012-08-26 | 2014-03-06 | 전라남도 | Formulated feeds for sea cucumber |
| CN105211550A (en) * | 2015-10-16 | 2016-01-06 | 大连工业大学 | A kind of preparation method of mixed culture solid state fermentation sea cucumber bait |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113974024A (en) * | 2021-11-01 | 2022-01-28 | 中国热带农业科学院热带生物技术研究所 | Sargassum liquid fermented feed additive and preparation method thereof and application in aquaculture |
| CN113974024B (en) * | 2021-11-01 | 2024-03-19 | 中国热带农业科学院热带生物技术研究所 | Sargassum liquid fermentation feed additive, preparation method thereof and application thereof in aquaculture |
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Application publication date: 20180518 |
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