CN102559535A - Grease degrading strain - Google Patents
Grease degrading strain Download PDFInfo
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- CN102559535A CN102559535A CN2011101689874A CN201110168987A CN102559535A CN 102559535 A CN102559535 A CN 102559535A CN 2011101689874 A CN2011101689874 A CN 2011101689874A CN 201110168987 A CN201110168987 A CN 201110168987A CN 102559535 A CN102559535 A CN 102559535A
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Abstract
The invention relates to a grease degrading strain. The grease degrading strain B5 belongs to a new strain of Paucimonas-lemoignei and was collected in China Center for Type Culture Collection (CCTCC) on May 30th, 2011, the collection number is CCTCC No: M2011523, and the collection address is Wuhan University in Wuhan. The grease degrading strain B5 has a biological strengthened treatment function of greasy wastewater under a low-temperature condition, the grease degrading rate of the strain can reach 95 percent, and secondary pollution to the environment is not produced; and the grease degrading strain is helpful to solve the problems of a greasy wastewater biological treatment system under the low-temperature condition, and has high application value.
Description
Technical field
The present invention relates to a kind of grease degradation bacteria.
Background technology
Grease is an important chemical material, is mainly used in glycerine and produces the preparation with higher fatty acid, and oil compounds can cause severe contamination and edible waste oil to abandon oil to ecotope and can threaten to HUMAN HEALTH.Therefore, the biological degradation of research oil compounds is significant for protection environment and raising human health.
The grease-contained waste water of low temperature belongs to one of unmanageable waste water in the industry.Domestic research to low temperature grease efficient degradation mikrobe is rarely found; Be directed to this; Grease efficient degrading bacteria under the screening coldcondition is also understood its degradation characteristic; The industrial treatment that can be low temperature oils waste water provides the microbiobacterial agent resource, thereby the problem that solves low temperature oils biological treatment of waste water system is laid the foundation.
Summary of the invention
The invention provides a kind of grease degradation bacteria.
The grease degradation bacteria, it belongs to Paucimonas-lemoignei and belongs to novel species for grease degraded B5; In China's typical culture collection center preservation; Deposit number is CCTCC No:M2011523, and the preservation address is a Wuhan City Wuhan University, and preservation date is on May 30th, 2011; It is the Gram-negative aerobic bacteria, and the thalline size is 0.8 μ m * 3.0 μ m; It forms circular bacterium colony on the PDA solid medium, pale pink, translucent, and smooth surface, it is wavy that the edge is, and is difficult for provoking; Enough utilize glucose, can not oxidation ethanol or aldose.
Grease degradation bacteria B5 of the present invention, the catalase reacting positive, produce indole test positive, methyl red test reaction negative, the starch hydrolysis is negative, fat hydrolysis is positive, nitrate reduction reaction negative, gelatine liquefication are negative, Hydrocerol A utilization feminine gender, sugar fermentating test are neither produced also aerogenesis not of acid.
Grease degradation bacteria B5 of the present invention compares through 16SrDNA order-checking and homology, and obtaining the kind the most close with it is Paucimonas-lemoignei, and homology reaches 98%; In conjunction with ne ar characteristic, growth conditions, Physiology and biochemistry qualification result, confirm that grease degradation bacteria B5 is the novel bacterial that Paucimonas-lemoignei belongs to.
Grease degradation bacteria B5 belongs to Paucimonas-lemoignei and belongs to novel species, and in China's typical culture collection center preservation, deposit number is CCTCC No:M2011523, and the preservation address is a Wuhan City Wuhan University, and preservation date is on May 30th, 2011.
Grease degradation bacteria B5 of the present invention has the biological reinforced processing capacity of oils waste water under the coldcondition, and the oil and grease degradation rate of bacterial strain can reach 95%, does not cause secondary environmental pollution; Problem for solving oils biological treatment of waste water system under the coldcondition is helpful, has higher using value.
Description of drawings
Fig. 1 is the atom mechanics microscopic examination figure of grease degradation bacteria B5 in the embodiment one, and Fig. 2 is the atom mechanics microscopic examination figure of grease degradation bacteria B5 in the embodiment one.
Embodiment
Embodiment one: this embodiment grease degradation bacteria B5; Belong to Paucimonas-lemoignei and belong to novel species; In China's typical culture collection center preservation; Deposit number is CCTCC No:M2011523, and the preservation address is a Wuhan City Wuhan University, and preservation date is on May 30th, 2011; It is the Gram-negative aerobic bacteria, and the thalline size is 0.8 μ m * 3.O μ m; It forms circular bacterium colony on the PDA solid medium, pale pink, translucent, and smooth surface, it is wavy that the edge is, and is difficult for provoking; Enough utilize glucose, can not oxidation ethanol or aldose.
Grease degradation bacteria B5 in this embodiment, its form is as illustrated in fig. 1 and 2; Its growth temperature is 5～15 ℃, and growth pH value is 5.5～9.5; Optimum growth temperature is 5 ℃, and the righttest growth pH value is 7.0.
According to " uncle Jie Shi bacteriology identification handbook " grease degradation bacteria B5 is carried out conventional Physiology and biochemistry and identifies that experimental result is: the catalase reacting positive, produce indole test positive, methyl red test reaction negative, the starch hydrolysis is negative, fat hydrolysis is positive, nitrate reduction reaction negative, gelatine liquefication are negative, Hydrocerol A utilization feminine gender, sugar fermentating test are neither produced also aerogenesis not of acid.
This embodiment grease degradation bacteria B5 is process domestication in the sewage water sample from the community resident living quarters, again through the overactivation primary dcreening operation, and then carries out activation and sieve again obtaining, and process is following:
The domestication process is that picking one collarium bacterial classification inoculation is in slant medium; Under the temperature of 15 ℃～5 ℃ of gradual changes, place illumination box to cultivate; Behind growth cycle of every cultivation again the inoculation in this slant medium of picking on other slant medium; Subculture rests on 5 ℃ gradually under the temperature of gradual change.5 ℃ of inoculation culture 2 months repeatedly, accomplish whole domestication process, obtain the facultative psychrophilic bacteria that 8 strains can stable growth breeding under 5 ℃ of conditions.1 ℃ of left and right sides preservation bacterial classification.
Primary dcreening operation is that the 8 strain bacterium that obtain are inoculated in respectively in the grease neutral red culture, carries out 5 ℃ low temperature and cultivates activation, after 2～3 activation, confirms to be inoculated in once more in the grease neutral red culture after bacterial classification has been replied vigor, cultivates after 4 days and observes.Can the meta-bolites that produce when observing colony growth in the substratum make the difference of toluylene red colour-change situation judge degrade grease or degrade greasy preliminary situation of this kind bacterium.The 8 strain bacterial strains that domestication obtains are cultivated the identical time under the same conditions; Then prove the growth that this bacterium can be good at low temperatures and have the greasy effect of degraded if wherein bigger the and color of single colony growth of a strain bacterium is scarlet, thereby rough determination goes out bacterial strain to greasy degraded situation.To this 8 strain bacterium random number 1-8 number, and carry out record, so that convenient record result multiple sieve the time.
Multiple sieve is the 8 strain bacterium that primary dcreening operation is used; Be inoculated in respectively with peanut oil is in the no carbon source substratum of sole carbon source; After taming several weeks, be inoculated in once more in the grease liquid nutrient medium and cultivate, after 4 days, choose the 3 strain bacterium that grow fine through observation; Continuation is that sole carbon source is cultivated with peanut oil; Activation utilizes 752 type ultraviolet spectrophotometers to filter out the bacterial strain preferably of growing at wavelength for the 580nm place surveys its OD value after 2 weeks, confirmed that through concrete experimental data 1 strain bacterium is a dominant bacteria, being numbered No. 5 of this bacterial classification.
Wherein every liter of slant medium is made up of 5gNaCl, 10g peptone and 5g Carnis Bovis seu Bubali cream, and the pH value is 7.0～7.2; Every liter of grease neutral red culture is made up of 5gNaCl, 10g peptone, 10g peanut oil, 5g Carnis Bovis seu Bubali cream, 20g agar and lmL toluylene red (1.6% neutral red aqueous solution), and the pH value is 7.2; The TSB substratum is made up of 30gTSB commercially available culture medium powder and 20g agar; Every liter is sieved substratum again by 5g (NH
4)
2SO
4, 2gK
2HPO
4, 0.5gMgSO
47H
2O, 2gNaCl, 2gNH
4H
2PO
4Form with 20g peanut oil.
The bacterium that filters out carries out conventional Physiology and biochemistry according to " uncle Jie Shi bacteriology identification handbook " to it identifies, adopts the CTAB method to carry out the extraction of bacteria total DNA.Gained bacterial genomes DNA is carried out pcr amplification with bacterial 16 S rDNA universal primer; The PCR product is reclaimed in rubber tapping behind the electrophoresis; Through Sherlock lipid acid identification systems No. 5 bacterial strains are detected evaluation and carry out homology relatively, find that the most close kind of bacterium that the screening of this embodiment obtains is Paucimonas-lemoignei, homology reaches 98%; In conjunction with ne ar characteristic, growth conditions, Physiology and biochemistry qualification result; Find to have any different, confirm as the novel bacterial that Paucimonas-lemoignei belongs to, called after grease degradation bacteria B5 with other bacterial classification of this genus.
In this embodiment, below the 1500mg/L grease concentration, when cultivating 24h, the degrading aniline rate reaches 40%; When cultivating 84h, the degrading aniline rate can reach more than 73.5%, and when cultivating 96h, the degrading aniline rate reaches 95%; Experiment proof grease efficient degrading bacteria B5 has the function of subzero treatment oil-containing waste water, and need not to add other chemicals, has broad application prospects.
Claims (1)
1. grease degradation bacteria; It is characterized in that grease degradation bacteria B5; Belong to Paucimonas-lemoignei and belong to novel species, in China's typical culture collection center preservation, deposit number is CCTCC No:M2011523; The preservation address is a Wuhan City Wuhan University, and preservation date is on May 30th, 2011.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101689874A CN102559535A (en) | 2011-06-22 | 2011-06-22 | Grease degrading strain |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101689874A CN102559535A (en) | 2011-06-22 | 2011-06-22 | Grease degrading strain |
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| CN102559535A true CN102559535A (en) | 2012-07-11 |
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| CN2011101689874A Pending CN102559535A (en) | 2011-06-22 | 2011-06-22 | Grease degrading strain |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105907666A (en) * | 2016-04-22 | 2016-08-31 | 浙江大学 | Preparation method and application of grease degrading bacterium community |
| CN106635871A (en) * | 2016-10-08 | 2017-05-10 | 浙江双良商达环保有限公司 | Complex bacteria and application thereof |
-
2011
- 2011-06-22 CN CN2011101689874A patent/CN102559535A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105907666A (en) * | 2016-04-22 | 2016-08-31 | 浙江大学 | Preparation method and application of grease degrading bacterium community |
| CN106635871A (en) * | 2016-10-08 | 2017-05-10 | 浙江双良商达环保有限公司 | Complex bacteria and application thereof |
| CN106635871B (en) * | 2016-10-08 | 2020-02-04 | 浙江双良商达环保有限公司 | Complex microbial inoculant and application thereof |
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Application publication date: 20120711 |