CN101603018A - Degraded oil, restoring petroleum polluted soil ecology bacteria preparation and preparation method thereof - Google Patents
Degraded oil, restoring petroleum polluted soil ecology bacteria preparation and preparation method thereof Download PDFInfo
- Publication number
- CN101603018A CN101603018A CNA200910069620XA CN200910069620A CN101603018A CN 101603018 A CN101603018 A CN 101603018A CN A200910069620X A CNA200910069620X A CN A200910069620XA CN 200910069620 A CN200910069620 A CN 200910069620A CN 101603018 A CN101603018 A CN 101603018A
- Authority
- CN
- China
- Prior art keywords
- preparation
- bacteria preparation
- polluted soil
- culture
- humic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of degraded oil, restoring petroleum polluted soil ecology bacteria preparation and preparation method thereof.The composite flora that the bacteria preparation of degraded oil, restoring petroleum polluted soil ecology is made up of through optimum combination bacillus megaterium, Pseudomonas fluorescens, streptococcus faecium and candida tropicalis, wherein the proportioning of each component is 1～2: 1: 1: 0.5～1.Positively effect of the present invention is: can the little ecological dominance flora of very fast formation after bacteria preparation is manured into soil.If add and (cultivate layer with respect to soil table soil, about 15～20cm) 2%～5% heavy microbial inoculums, effective colony number height of oil removal rate in three months 〉=75% (methylene chloride reflux is extracted, weighting method) bacteria preparation, adaptability is strong, and the restoring petroleum polluted soil ecology environment is had tangible effect.
Description
Technical field
Biological technical field the present invention relates to bacteria preparation of a kind of degraded oil, restoring petroleum polluted soil ecology and preparation method thereof.
Background technology
In oil production, transportation and use, inevitably can cause pollution, especially pollution to be on the rise to soil ecosystem to surrounding environment.For the reparation of petroleum polluted soil ecology, more existing both at home and abroad researchs still, yet there are no high-density, high reactivity, the report of the degraded oil that energy original position big area is used, the bacteria preparation of restoring petroleum polluted soil ecology.
Summary of the invention
Technical problem to be solved by this invention is: bacteria preparation of a kind of high-density, highly active degraded oil, restoring petroleum polluted soil ecology and preparation method thereof is provided.
The present invention solves this technical problem the technical scheme that is adopted:
Utilize subtilis (Bacillus subtilis), Pseudomonas fluorescens (Pseudomonas fluorescens), streptococcus faecium (Enteococccus faecalis) and candida tropicalis (Candida tropicalis), it all is the bacterium that can produce oxide compound enzyme, desaturase etc. and lipopolysaccharides tensio-active agent, can be near degraded oil component progressively when being adsorbed in oil on the soil particle, thus reach the purpose of degraded oil; Because these four kinds of bacterium all come from soil, thus can very fast formation dominant microflora after entering soil, occupy certain ecological niche.These four kinds of bacterium also have the effect that promotes plant-root growth, improves the rhizosphere ecotope, therefore, if adopt microbial inoculum-plant combined recovery technique, will promote the reparation of petroleum polluted soil ecology.With the activation of four kinds of bacterium through three grades of fluid enlargement culture and adsorb, be immobilized onto and be prepared into bacteria preparation on the humic acid.Four strain bacterial classifications are all purchased the DSMZ in Institute of Microorganism, Academia Sinica.
The bacteria preparation of degraded oil, restoring petroleum polluted soil ecology is the floras of above-mentioned four kinds of bacterium through the composition of optimum combination, and wherein the proportioning of each component is 1: 1: 1 basically: 1. these four kinds of bacterium all are safe, the harmless bacterium that the Ministry of Agriculture allows use.
The preparation method of the bacteria preparation of degraded oil, restoring petroleum polluted soil ecology comprises the steps:
1. each slant strains activation, the shake-flask culture respectively that will preserve; Minimum medium: Zulkovsky starch culture medium culturing, pH7.0～7.2,0.1Mpa, 121 ℃ of autoclaving 20～30min; Cultivate 12～24h for 28～30 ℃;
2. each flora with above-mentioned shake-flask culture mixes, secondary fluid enlargement culture (20L jar), and inoculum size 5%～10% is cultivated 12～24h for 28～30 ℃;
3. again through three grades of fluid enlargement culture (200L jar), inoculum size 5%～10% is cultivated 12～24h for 28～30 ℃;
4. be that carrier evenly adsorbs mixed bacterial, is immobilized onto on 3 ~ 5 times of humic acid with the humic acid powder.
5. be sealed in the packing bag of lined with polyethylene room temperature preservation, 1 year quality guaranteed period.
Beneficial effect of the present invention
1) composite bacteria is immobilized onto on the humic acid, makes effective bacterium colony number (cfu) of every gram bacteria preparation can be up to 100 more than hundred million (promptly 1 ~ 3 * 10
10/ g), almost reached most probable number MPN, so far yet there are no the report of high bacteria containing amount bacteria preparation like this. humic acid can not only provide some nutritive ingredients by being cultivated bacterium, also because its reticulated structure, has bigger specific surface area, so can in unit volume, adhere to, thalline that adsorption of immobilization is more.Therefore, in a single day a certain amount of effective bacteria agent is manured into soil can very fast formation dominant microflora, the effect of performance degraded oil component, restoring petroleum polluted soil ecology.
2) because the petroleum component of contaminated soil complicated component not only, and because of oil is adsorbed on the soil particle securely, single bacterium or a spot of bacterium are difficult to carry out the petroleum component degraded.The degraded fully of petroleum component must could eventual degradation be water and carbonic acid gas by multiple microorganisms plurality of enzymes.Especially the polycyclic aromatic hydrocarbons of difficult degradation must could open loop by bacteriogenic dioxygenase and mycetogenetic monooxygenase effect, otherwise polycyclic aromatic hydrocarbons just is difficult to begin degraded.
3) mensuration of oil removal rate is with 54 ℃ of backflows of methylene dichloride, 12 ~ 20h, the rough vacuum rotary evaporation is removed methylene dichloride, weighting method is weighed and is calculated and adds microbial inoculum and not with the oil length of microbial inoculum pedotheque, the difference of the two is exactly an oil removal rate, if add and (cultivate layer with respect to soil table soil, about 15～20cm) 2%～5% heavy microbial inoculums, oil removal rate in three months 〉=75% (methylene chloride reflux is extracted, weighting method).
4) soil ecology reparation PCR-DGGE (DNA cloning-denaturing gradient gel electrophoresis) method evaluation, the result shows that microbial diversity obviously increases, as shown in Figure 1 and Figure 2, Fig. 1 denaturing gradient gel electrophoresis figure, Fig. 2 is the Quantity one software processes figure of Fig. 1.
Description of drawings
Fig. 1 denaturing gradient gel electrophoresis figure
Fig. 2 is the Quantity one software processes figure of Fig. 1
1-0d, 2-8d, 3-16d, 4-32d, the electrophoretic band of expression different number of days.
Embodiment
Follow these steps to carry out:
1) four kinds of strain inclined planes with 4 ℃ of preservations activate, respectively shake-flask culture.With the Zulkovsky starch culture medium culturing, it is as follows specifically to fill a prescription: Zulkovsky starch 20g, KNO
31g, NaCl 0.5g, K
2HPO
40.5g, MgSO
4.7H
2O 0.5g, FeSO
40.01g, distilled water 1000mL, pH7.2,0.1Mpa, 121 ℃ of autoclaving 30min.Cultivate 24h for 28 ℃.
2) secondary fluid enlargement culture (6L) replaces Zulkovsky starch in the above-mentioned substratum with Semen Maydis powder, and adds 0.1% humic acid (massfraction), and inoculum size is cultivated 24h for 5%, 28 ℃.。
3) three grades of fluid enlargement culture (60L), substratum is the same; Inoculum size is cultivated 24h for 10%, 28 ℃.
4) with the humic acid carrier thereon with liquid-solid fixed, the absorption of bacterium.Be sealed in the polyethylene bag room temperature preservation.
Follow these steps to carry out:
1) four kinds of strain inclined planes with 4 ℃ of preservations activate, respectively shake-flask culture.With Zulkovsky starch substratum enlarged culturing, it is as follows specifically to fill a prescription: Zulkovsky starch 20g, KNO
31g, NaCl 0.5g, K
2HPO
40.5g, MgSO
4.7H
2O 0.5g, FeSO
40.01g, distilled water 1000mL, pH7.0,0.1Mpa, 121 ℃ of autoclaving 30min.Cultivate 12h for 28 ℃.
2) secondary fluid enlargement culture (10L) replaces Zulkovsky starch in the above-mentioned substratum with Semen Maydis powder, and adds 0.1% humic acid (massfraction), and inoculum size is cultivated 12h for 5%, 28 ℃.。
3) three grades of fluid enlargement culture (100L), substratum is the same, and inoculum size is cultivated 12h for 10%, 28 ℃.
4) be carrier with the humic acid with bacterium liquid-solid fixed, be adsorbed on the humic acid.Be sealed in the polyethylene bag room temperature preservation.
Follow these steps to carry out:
1) three kinds of strain inclined planes with 4 ℃ of preservations activate, respectively shake-flask culture.With Zulkovsky starch substratum enlarged culturing, it is as follows specifically to fill a prescription: Zulkovsky starch 20g, KNO
31g, NaCl 0.5g, K
2HPO
40.5g, MgSO
4.7H
2O 0.5g, FeSO
40.01g, distilled water 1000mL, pH7.2,0.1Mpa, 121 ℃ of autoclaving 30min.Cultivate 12 ~ 24h for 28 ℃.
2) secondary fluid enlargement culture (20L) replaces Zulkovsky starch in the above-mentioned substratum with Semen Maydis powder, and adds 0.1% humic acid (massfraction), and inoculum size is cultivated 24h for 5%, 28 ℃.。
3) three grades of fluid enlargement culture (200L), substratum is the same, and inoculum size is cultivated 12 ~ 24h for 10%, 28 ℃.
4) be carrier with the humic acid with bacterium liquid-solid fixed, be adsorbed on the humic acid.Be sealed in the polyethylene bag room temperature preservation.
Claims (2)
- One kind can the degraded oil restoring petroleum polluted soil ecology bacteria preparation, it is characterized in that: the composite flora that this bacteria preparation is made up of through optimum combination subtilis (Bacillus subtilis), Pseudomonas fluorescens (Pseudomonas fluorescens), streptococcus faecium (Enterococcusfaecalis) and candida tropicalis (Candida tropicalis), wherein the proportioning of each bacterium component is 1～2: 1: 1: 0.5～1.
- 2. the preparation method of the bacteria preparation of degraded oil restoring petroleum polluted soil ecology as claimed in claim 1 is characterized in that it comprises the steps:1) with subtilis (Bacillus subtilis), Pseudomonas fluorescens (Pseudomonasfluorescens), streptococcus faecium (Enterococcus faecalis) and candida tropicalis (Candida tropicalis) the face actication of culture preserved, the difference shake-flask culture; Minimum medium: Zulkovsky starch culture medium culturing, pH7.0～7.2,0.1Mpa, 121 ℃ of autoclaving 20～30min; Cultivate 12～24h for 28～30 ℃;2) each flora with above-mentioned shake-flask culture mixes, the secondary fluid enlargement culture, cumulative volume 5～20L, with the Zulkovsky starch in the Semen Maydis powder replacement Zulkovsky starch substratum, and add 0.1% humic acid, other compositions are identical with the Zulkovsky starch substratum, and the inoculum size volume percent is 5%～10%, 28～30 ℃ and cultivates 12～24h;3) again through three grades of fluid enlargement culture, 10 times of cumulative volumes are to 2), the enlarged culturing base is with 2), the inoculum size volume percent is 5%～10%, 28～30 ℃ and cultivates 12～24h;4) be that carrier evenly adsorbs mixed bacterial, is immobilized onto on the humic acid with the humic acid, the enlarged culturing liquid weight ratio of humic acid and step 3) is 3 ~ 5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200910069620XA CN101603018A (en) | 2009-07-07 | 2009-07-07 | Degraded oil, restoring petroleum polluted soil ecology bacteria preparation and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200910069620XA CN101603018A (en) | 2009-07-07 | 2009-07-07 | Degraded oil, restoring petroleum polluted soil ecology bacteria preparation and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101603018A true CN101603018A (en) | 2009-12-16 |
Family
ID=41468921
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA200910069620XA Pending CN101603018A (en) | 2009-07-07 | 2009-07-07 | Degraded oil, restoring petroleum polluted soil ecology bacteria preparation and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101603018A (en) |
Cited By (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101890426A (en) * | 2010-07-19 | 2010-11-24 | 南开大学 | Method for restoring petroleum-polluted saline alkali soil by ryegrass and high efficiency microbes |
| CN101973641A (en) * | 2010-10-09 | 2011-02-16 | 江苏博大环保股份有限公司 | Hyposmosis/super-hyposmosis oil extraction sewage reinjection method in oil field |
| CN101993177A (en) * | 2010-10-13 | 2011-03-30 | 江苏博大环保股份有限公司 | Method for treating polymer-containing sewage of oil field |
| CN102190373A (en) * | 2010-03-08 | 2011-09-21 | 江苏中超环保有限公司 | Aerobic and facultative anaerobic microbial activated bactericide for water treatment and application thereof in contact oxidation process |
| CN102250801A (en) * | 2011-06-22 | 2011-11-23 | 中国农业科学院农业环境与可持续发展研究所 | Microbial agent, and its preparation method and application |
| CN102453678A (en) * | 2010-10-26 | 2012-05-16 | 中国科学院生态环境研究中心 | Microorganism composite bacterial agent for restoration of saline alkali soil polluted by petroleum |
| CN102659252A (en) * | 2012-06-05 | 2012-09-12 | 山东大学 | Treatment method of wastewater containing petroleum |
| CN102925390A (en) * | 2012-11-05 | 2013-02-13 | 上海交通大学 | Pseudomonas fluorescens capable of decomposing petroleum substances and preparation method and application thereof |
| CN101857840B (en) * | 2010-05-12 | 2013-02-27 | 上海交通大学 | Biological flora suspending agent for degrading petroleum and preparation method and application method thereof |
| CN103045582A (en) * | 2013-01-21 | 2013-04-17 | 南开大学 | Method for preparing anti-cropping ecological fungicide for repairing panax notoginseng continuous cropping soil |
| RU2499636C1 (en) * | 2012-03-20 | 2013-11-27 | Общество с ограниченной ответственностью "Уралэкоресурс" (ООО "Уралэкоресурс") | Method of biological reclamation of oil-contaminated soils |
| CN103480644A (en) * | 2013-10-12 | 2014-01-01 | 南开大学 | Plant-microorganism combined method for enhanced repairing of petroleum-contaminated spetroleum |
| RU2529735C1 (en) * | 2013-01-30 | 2014-09-27 | Общество с ограниченной ответственностью "Уралэкоресурс" (ООО "Уралэкоресурс") | Method of producing biopreparation for cleaning and restoring fertility of soil contaminated with petroleum products |
| CN104140962A (en) * | 2013-10-29 | 2014-11-12 | 中国石油化工集团公司 | Immobilization method for petroleum degrading bacterial group |
| CN104774797A (en) * | 2015-04-30 | 2015-07-15 | 大连民族学院 | Low-temperature bacterium BW02 with petroleum degradation function, purpose thereof and seafloor sediment petroleum degradation bacterium agents |
| CN104774832A (en) * | 2015-04-30 | 2015-07-15 | 大连民族学院 | Seabed sedimentation oil decomposing bactericide and preparation method thereof |
| CN104830721A (en) * | 2015-04-30 | 2015-08-12 | 大连民族学院 | Bacterium AJ07 having petroleum degradation function, use thereof, and seabed settlement petroleum degradation bacterium agent |
| CN104830720A (en) * | 2015-04-30 | 2015-08-12 | 大连民族学院 | Bacterial strain AW29 suitable for petroleum degradation, use thereof, and seabed settlement petroleum degradation bacterial agent |
| CN105483058A (en) * | 2016-01-09 | 2016-04-13 | 国家海洋局北海环境监测中心 | Petroleum-degrading bactericide and preparation method thereof |
| CN105567580A (en) * | 2015-12-31 | 2016-05-11 | 沈阳化工大学 | Sophorolipid producing microzyme, and preparation method and application thereof |
| CN106635871A (en) * | 2016-10-08 | 2017-05-10 | 浙江双良商达环保有限公司 | Complex bacteria and application thereof |
| CN106734185A (en) * | 2016-12-28 | 2017-05-31 | 深圳市中兰环保科技股份有限公司 | A kind of soil remediation method |
| CN107475163A (en) * | 2017-09-28 | 2017-12-15 | 无锡盛雅生物科技有限公司佛山分公司 | The method that microbe handles oil pollution soil |
| CN108753658A (en) * | 2018-06-19 | 2018-11-06 | 南京工程学院 | A kind of benzo [a] pyrene degradation bacteria and their usage and use and the minimal medium and its preparation method for preparing the bacterial strain |
| CN109182211A (en) * | 2018-10-16 | 2019-01-11 | 中国石油化工股份有限公司 | The composite bacteria agent and application method of SRB are prevented and treated under wide temperature environment |
| CN110591985A (en) * | 2019-10-29 | 2019-12-20 | 浙江黛君生物医药科技有限公司 | Microbial symbiotic culture microbial inoculum and preparation method and application thereof |
| CN113617829A (en) * | 2021-08-01 | 2021-11-09 | 重庆工商大学 | Biological oil removal method for soil containing heavy metals and waste engine oil |
-
2009
- 2009-07-07 CN CNA200910069620XA patent/CN101603018A/en active Pending
Cited By (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102190373A (en) * | 2010-03-08 | 2011-09-21 | 江苏中超环保有限公司 | Aerobic and facultative anaerobic microbial activated bactericide for water treatment and application thereof in contact oxidation process |
| CN102190373B (en) * | 2010-03-08 | 2012-12-26 | 江苏中超环保有限公司 | Aerobic and facultative anaerobic microbial activated bactericide for water treatment and application thereof in contact oxidation process |
| CN101857840B (en) * | 2010-05-12 | 2013-02-27 | 上海交通大学 | Biological flora suspending agent for degrading petroleum and preparation method and application method thereof |
| CN101890426A (en) * | 2010-07-19 | 2010-11-24 | 南开大学 | Method for restoring petroleum-polluted saline alkali soil by ryegrass and high efficiency microbes |
| CN101890426B (en) * | 2010-07-19 | 2011-11-30 | 南开大学 | Method for restoring petroleum-polluted saline alkali soil by ryegrass and high efficiency microbes |
| CN101973641A (en) * | 2010-10-09 | 2011-02-16 | 江苏博大环保股份有限公司 | Hyposmosis/super-hyposmosis oil extraction sewage reinjection method in oil field |
| CN101973641B (en) * | 2010-10-09 | 2012-12-05 | 江苏博大环保股份有限公司 | Hyposmosis/super-hyposmosis oil extraction sewage reinjection method in oil field |
| CN101993177A (en) * | 2010-10-13 | 2011-03-30 | 江苏博大环保股份有限公司 | Method for treating polymer-containing sewage of oil field |
| CN101993177B (en) * | 2010-10-13 | 2013-05-01 | 江苏博大环保股份有限公司 | Method for treating polymer-containing sewage of oil field |
| CN102453678A (en) * | 2010-10-26 | 2012-05-16 | 中国科学院生态环境研究中心 | Microorganism composite bacterial agent for restoration of saline alkali soil polluted by petroleum |
| CN102250801A (en) * | 2011-06-22 | 2011-11-23 | 中国农业科学院农业环境与可持续发展研究所 | Microbial agent, and its preparation method and application |
| CN102250801B (en) * | 2011-06-22 | 2013-11-06 | 中国农业科学院农业环境与可持续发展研究所 | Microbial agent, and its preparation method and application |
| RU2499636C1 (en) * | 2012-03-20 | 2013-11-27 | Общество с ограниченной ответственностью "Уралэкоресурс" (ООО "Уралэкоресурс") | Method of biological reclamation of oil-contaminated soils |
| CN102659252A (en) * | 2012-06-05 | 2012-09-12 | 山东大学 | Treatment method of wastewater containing petroleum |
| CN102925390A (en) * | 2012-11-05 | 2013-02-13 | 上海交通大学 | Pseudomonas fluorescens capable of decomposing petroleum substances and preparation method and application thereof |
| CN103045582A (en) * | 2013-01-21 | 2013-04-17 | 南开大学 | Method for preparing anti-cropping ecological fungicide for repairing panax notoginseng continuous cropping soil |
| RU2529735C1 (en) * | 2013-01-30 | 2014-09-27 | Общество с ограниченной ответственностью "Уралэкоресурс" (ООО "Уралэкоресурс") | Method of producing biopreparation for cleaning and restoring fertility of soil contaminated with petroleum products |
| CN103480644A (en) * | 2013-10-12 | 2014-01-01 | 南开大学 | Plant-microorganism combined method for enhanced repairing of petroleum-contaminated spetroleum |
| CN104140962A (en) * | 2013-10-29 | 2014-11-12 | 中国石油化工集团公司 | Immobilization method for petroleum degrading bacterial group |
| CN104830720B (en) * | 2015-04-30 | 2017-12-29 | 大连民族学院 | Strains A W29 and its purposes and seabed sedimentation oil degradation microbial inoculum suitable for oil degradation |
| CN104774832A (en) * | 2015-04-30 | 2015-07-15 | 大连民族学院 | Seabed sedimentation oil decomposing bactericide and preparation method thereof |
| CN104830721A (en) * | 2015-04-30 | 2015-08-12 | 大连民族学院 | Bacterium AJ07 having petroleum degradation function, use thereof, and seabed settlement petroleum degradation bacterium agent |
| CN104830720A (en) * | 2015-04-30 | 2015-08-12 | 大连民族学院 | Bacterial strain AW29 suitable for petroleum degradation, use thereof, and seabed settlement petroleum degradation bacterial agent |
| CN104774797A (en) * | 2015-04-30 | 2015-07-15 | 大连民族学院 | Low-temperature bacterium BW02 with petroleum degradation function, purpose thereof and seafloor sediment petroleum degradation bacterium agents |
| CN105567580A (en) * | 2015-12-31 | 2016-05-11 | 沈阳化工大学 | Sophorolipid producing microzyme, and preparation method and application thereof |
| CN105567580B (en) * | 2015-12-31 | 2019-04-02 | 沈阳化工大学 | One plant of production sophorolipid saccharomycete and the preparation method and application thereof |
| CN105483058A (en) * | 2016-01-09 | 2016-04-13 | 国家海洋局北海环境监测中心 | Petroleum-degrading bactericide and preparation method thereof |
| CN105483058B (en) * | 2016-01-09 | 2019-09-17 | 国家海洋局北海环境监测中心 | A kind of oil degradation microbial inoculum and preparation method thereof |
| CN106635871A (en) * | 2016-10-08 | 2017-05-10 | 浙江双良商达环保有限公司 | Complex bacteria and application thereof |
| CN106734185A (en) * | 2016-12-28 | 2017-05-31 | 深圳市中兰环保科技股份有限公司 | A kind of soil remediation method |
| CN107475163A (en) * | 2017-09-28 | 2017-12-15 | 无锡盛雅生物科技有限公司佛山分公司 | The method that microbe handles oil pollution soil |
| CN108753658A (en) * | 2018-06-19 | 2018-11-06 | 南京工程学院 | A kind of benzo [a] pyrene degradation bacteria and their usage and use and the minimal medium and its preparation method for preparing the bacterial strain |
| CN108753658B (en) * | 2018-06-19 | 2021-11-12 | 南京工程学院 | Benzo [ a ] pyrene degrading bacterium, use method and application thereof, inorganic salt culture medium for preparing strain and preparation method of inorganic salt culture medium |
| CN109182211A (en) * | 2018-10-16 | 2019-01-11 | 中国石油化工股份有限公司 | The composite bacteria agent and application method of SRB are prevented and treated under wide temperature environment |
| CN109182211B (en) * | 2018-10-16 | 2021-09-07 | 中国石油化工股份有限公司 | Composite biological agent for preventing and treating SRB in wide-temperature environment and use method |
| CN110591985A (en) * | 2019-10-29 | 2019-12-20 | 浙江黛君生物医药科技有限公司 | Microbial symbiotic culture microbial inoculum and preparation method and application thereof |
| CN113617829A (en) * | 2021-08-01 | 2021-11-09 | 重庆工商大学 | Biological oil removal method for soil containing heavy metals and waste engine oil |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101603018A (en) | Degraded oil, restoring petroleum polluted soil ecology bacteria preparation and preparation method thereof | |
| CN100487108C (en) | Solid microbe agent for degrading petroleum pollution, and petroleum products, and preparation method | |
| CN102888374B (en) | Biological agent for processing industrial sewage and preparation method and application | |
| CN103436464B (en) | Low temperature-resistant petroleum-degrading bacillus sp. strain, culture method and application thereof | |
| CN101134955A (en) | Solid composite micro-organism micro-balloon for organic contaminant biodegradation and method for preparing the same | |
| CN102533619B (en) | Preparation method of hydrochloric ether efficient aerobic degradation mixed bacteria and application thereof | |
| CN101230322A (en) | Novel medical stone micro-ecological repairing additive and preparation method thereof | |
| CN103215204B (en) | Arthrobacter strain highly effectively degrading phenanthrene, and application thereof | |
| CN101724582A (en) | Immobilized microbial inoculum for remediating PAHs contaminated soil and preparation method thereof | |
| CN108587972B (en) | Bacillus thuringiensis MRP-3 for adsorbing heavy metals and application thereof | |
| CN101914444A (en) | Ecological microbial inoculum for remedying oil-polluted soils and preparation method thereof | |
| CN102277312A (en) | Strain for degrading polycyclic aromatic hydrocarbons at low temperature and application in bioremediation of groundwater in petroleum hydrocarbon polluted places thereof | |
| Sharma et al. | Effect of grain size on bacterial penetration, reproduction, and metabolic activity in porous glass bead chambers | |
| CN103045579A (en) | Microbial remediation curing adsorbing bacterial preparation applicable to marine environment petroleum pollution as well as preparation method and application of same | |
| Nwankwegu et al. | Microbial cell immobilization: a renaissance to bioaugmentation inadequacies. A review | |
| CN108441451A (en) | Complex microorganism soil-repairing agent and its preparation method and application | |
| CN104649848A (en) | Solid bacterial fertilizer for remedying petroleum polluted saline alkali soil and preparation method of solid bacterial fertilizer | |
| CN108467844B (en) | Pseudomonas putida MRP-2 and application thereof | |
| Wyss et al. | Micro-encapsulated organic phase for enhanced bioremediation of hydrophobic organic pollutants | |
| CN104789506B (en) | The sea rotation bacterium of polycyclic aromatic hydrocarbon and its application in one plant of degradable salt environment | |
| CN102286399B (en) | Rhodococcus sp.P52 capable of degrading dibenzofuran and use thereof | |
| CN101935631B (en) | Ralstoniasp. and application thereof in bioremediation of petroleum-contaminated saline-alkali soil | |
| CN103013885A (en) | Benzene composite degrading microbe and immobilized benzene composite microbial inoculum, and preparation method thereof | |
| CN104988087B (en) | Oil contaminated soil complex microorganism renovation agent and preparation method thereof | |
| CN101531974A (en) | Arctic bacteria strain for highly efficiently degrading crude oil and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20091216 |