CN102250805A - High-density culture method of composite microbial agent for papermaking wastewater treatment - Google Patents
High-density culture method of composite microbial agent for papermaking wastewater treatment Download PDFInfo
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- CN102250805A CN102250805A CN 201110183629 CN201110183629A CN102250805A CN 102250805 A CN102250805 A CN 102250805A CN 201110183629 CN201110183629 CN 201110183629 CN 201110183629 A CN201110183629 A CN 201110183629A CN 102250805 A CN102250805 A CN 102250805A
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Abstract
The invention provides a high-density culture method of a composite microbial agent for papermaking wastewater treatment. The high-density culture method comprises the steps of: sequentially inoculating a high-density fermentation base culture medium with Phanerochaete chrysosporium ATCC20696, Nocardia coralline ACCC 40100 and Pseudomonas aeruginosa and carrying out fermentation culture under a certain condition for 80-100 hours. The high-density culture method provided by the invention has the characteristics that: the designed culture medium has low price (which is only about 20% of an LB culture medium) and meets the industrial high-density fermentation requirements of the composite microbial agent; the designed fermentation process control conditions reach the high-density production requirements of the composite microbial agent and achieve the purposes that the investment in the equipment and other production factors is saved and the strain ratio and activity of the composite microbial agent after fermentation meet the requirements; and according to the control points of the high-density culture method, the three strains can completely meet the required proportion after the fermentation is finished and the three strains are adapted to each other and maintain the respective activity and pollutant degrading capability, thus laying a foundation for the large-scale production and application of the composite microbial agent.
Description
(1) technical field
The present invention relates to a kind of papermaking wastewater and handle the high-density cultivation method of using composite fungus agent.
(2) background technology
Papermaking wastewater is handled the general second-stage treatment technology of using physical treatment process and biological treatment to combine, and wherein biological treatment mainly is to utilize active sludge to come pollution composition in the degradation of sewage.In real production implementation process, some inconvenience or defective that activated sludge process usually runs into, such as:
1. winter low temperature, toxicant, load is high, and high salinity often causes system crash.
2. nitrobacteria is difficult to cultivate, the nitrification instability, and the water outlet ammonia nitrogen is not up to standard.
3. complicated organic compositions such as aromatic series, xylogen can't be by efficient degradation in the water.
4. the biochemical system impact resistance is low, poor stability.
In order to address the above problem, the bio-synergistic technology is applied to the various sewage treatment process that comprise paper-making industrial waste water.Bio-synergistic technology (Bioaugmentation technique) is a kind ofly to have the biological flora of specific degradation capability by adding, strengthens the high-tech biotechnology of Sewage treatment systems self treatment effect.The bacterial strain that has specific degradation function by interpolation has been strengthened the ability of indigenous microorganism anti-shock loading and some compound of degrading to strengthen the effect of native bacterium.The key of bio-synergistic technology is to screen the corresponding microorganism of corresponding specific pollutants and realize suitability for industrialized production, and the research of bacterial screening aspect at present is more, but generally rests on laboratory stage, applies to industrial few.So the large-scale production research to specific microbial inoculum is still waiting further reinforcement.
The high-density culture technology, also claim the high density fermentation technology in the industrial microorganism field, be a kind of microorganism culturing technology that the certain culture technology and equipment improves thalline biomass and target product space-time yield of using, now be successfully applied in the production of genetic engineering bacterium and product thereof.The approach that realizes high density fermentation mainly contains: 1. the optimization of substratum composition; 2. the interpolation of particular nutrient; 3. the accumulation of restriction metabolic by-prods; 4. flow feeding strategy etc.The large-scale production that develops into various microbial inoculums of high density fermentation technology provides feasible approach, so will become a focus direction in microbial inoculum research and development field at the high density fermentation technical study of different purposes microorganisms.
At present, the production method of composite fungus agent mainly is by each composition bacterial classification that ferments respectively, and then is mixed and made into microbial inoculum according to a certain percentage.The advantage of this production method is: according to the characteristics of different strain, select different substratum and fermentation condition, can reach the production optimization of various bacterium, and the component proportions of final microbial inoculum is easy to control; But this method also has certain deficiency, as: the wherein each kind of difference such as bacterium active condition of many, the mixed microbial inoculums of production factors such as equipment that needs will have one period adaptive phase or occur producing microbial inoculum viable bacteria ratio owing to competitive power is different bigger variation etc. to occur after adding.
(3) summary of the invention
The objective of the invention is to design a kind of high-density culture technology that is exclusively used in the biological potentiating agent of papermaking wastewater processing, comprise a kind of fermentating controling process condition that is suitable for the substratum of this synergistic agent of suitability for industrialized production and produces this synergistic agent, thereby reach the investment of production factors such as saving equipment and the effects such as stability of raising composite fungus agent.
The technical solution used in the present invention is:
A kind of papermaking wastewater is handled the high-density cultivation method with composite fungus agent, described method comprises: inoculate Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC 20696, coral promise cassette bacterium (Nocardia coralline) ACCC40100 successively in basic medium and Pseudomonas aeruginosa (Pseudomonas aeruginosa) CGMCC No.1.239 carries out fermentation culture at high density fermentation, controlled temperature is that 28～32 ℃, pH5.0～7.5, dissolved oxygen are 1～5mgL
-1, in the fermenting process in batches stream to add cumulative volume be high density fermentation with the glucose solution of the mass concentration 50～60% of basic medium volume 10～20%, fermentation total time is 80～100h; Described high density fermentation is composed as follows with basic medium: glucose 10～15gL
-1, soy peptone 10～15gL
-1, SODIUM PHOSPHATE, MONOBASIC 8～12gL
-1, vitamins B
12～5mgL
-1, vitamin H 0.1～0.5mgL
-1, micro-mother liquor 1～3mLL
-1, fermentation is with defoamer (the efficient polyether antifoam agent of PPE, Zhejiang University chemical plant) 0.1mLL
-1, regulate pH to 5.5 with phosphoric acid; Described micro-mother liquor is composed as follows: C
oCl
26H
2O 1mgml
-1, MnCl
24H
2O 6mgml
-1, CuCl
22H
2O 0.6mgml
-1, H
3BO
41.2mgml
-1, Na
2M
oO
42H
2O 1mgml
-1, Zn (CH
3COO)
22H
2O5.2mgml
-1, FeC
6H
5O
75H
2O 40mgml
-1Composite fungus agent contains 3 kinds of microorganisms, all is to have the ability of specific pollutants in the papermaking wastewater of degrading and nuisanceless.The bacterial classification component proportions (w/w) that obtains after the fermentation is: Phanerochaete chrysosporium 15～25%; Pseudomonas aeruginosa 65～75%; Coral promise cassette bacterium 5～15%.The best preferred value of the content of above-mentioned three kinds of bacterium: Phanerochaete chrysosporium content 20%; Pseudomonas aeruginosa content 70%; Coral promise cassette bacterial content 10%.
Concrete, cultivate 24h behind the inoculation Phanerochaete chrysosporium, inoculate coral promise cassette bacterium, inoculate Pseudomonas aeruginosa after continuing to cultivate 24h.
Described glucose solution begins stream and adds behind inoculation Pseudomonas aeruginosa 24h, stream adds and finishes in 36h, but the mode that the fed-batch mode selectivity index increases.
Preferably, control pH inoculates behind the coral promise cassette bacterium until fermentation ends control pH 7.0 ± 0.3 5.5 ± 0.3 behind the yellow archespore hair lead fungi of inoculation, and pH regulator liquid is ammoniacal liquor in the fermenting process.
Described high density fermentation becomes with the basic medium best group: glucose 10gL
-1, soy peptone 10gL
-1, SODIUM PHOSPHATE, MONOBASIC 10gL
-1, vitamins B
13mgL
-1, vitamin H 0.4mgL
-1, micro-mother liquor 2.5mLL
-1, fermentation is with defoamer (the efficient polyether antifoam agent of PPE, Zhejiang University chemical plant) 0.1mLL
-1, regulate pH to 5.5 with phosphoric acid.
Fermentation technology process is as follows:
(1) flat board of bacterial classification activation:
Take out being kept at-80 ℃ of bacterial classifications in the refrigerator, melt on ice, aseptic technique is inoculated in the corresponding plate culture medium respectively, and 30 ℃ of constant temperature culture 48～72h are standby.
(2) seed liquor preparation:
The single bacterium colony on the picking plate culture medium respectively, aseptic inoculation in the triangular flask that corresponding substratum is housed, 30 ℃, on horizontal shaking table, cultivate 24～36h with suitable rotating speed.
(3) go up a jar fermentation
At first the 6.5L basic medium is put into the 10L fermentor tank, 121 ℃ of sterilization 25min, treat substratum cooling after, feed sterile air and stir 5min with the rotating speed of 180rpm.Stop ventilation, the dissolved oxygen levels that be set this moment is 100% (to be about 7～8mgL
-1).
The Phanerochaete chrysosporium seed liquor is inoculated in the fermentor tank according to about 2% volume ratio, control pH (is provided with the fermentor tank sequence of control in 5.5 ± 0.3 level, determine the interpolation speed of ammoniacal liquor by the peristaltic pump of on-line detecting system control), 30 ℃ of temperature begin fermentation; Inoculate coral promise cassette bacterium by same inoculum size again behind the 24h, regulate pH to 7.0 ± 0.3 before the inoculation, continue fermentation 24h; Inoculate pseudomonas aeruginosa then, begin feed supplement behind the cultivation 24h.Three kinds of microorganisms of feed supplement mode reference enter the opportunity of logarithmic phase, add according to the speed of exponential form, and the feed supplement time that the feed supplement amount of 1L is set is 36h, after feed supplement finishes, continue to finish fermentation culture behind the fermentation 2h.In the whole fermentation culture process, control dissolved oxygen levels by the mode that changes rotating speed of agitator and feeding pressurized air or pure oxygen and (be about 2mgL 30%
-1) more than.After the fermentation ends, dry cell weight reaches 22.46gL in the fermented liquid
-1And more than.
Beneficial effect of the present invention is mainly reflected in: the substratum that designs among the present invention has cheap (only for LB substratum about 20%), satisfies the industrialization high density fermentation requirement of this composite fungus agent; The high density production that the zymotechnique control condition of design has reached composite fungus agent requires that (after the fermentation ends, dry cell weight reaches 22.46gL in the fermented liquid
-1And more than), the investment that has realized production factors such as saving equipment is (if three kinds of compositions are produced respectively, at least want three cover fermentation equipment to use simultaneously or same set of equipment wants three production cycles just can reach identical effect, that is to say that the investment of the production means such as equipment can be saved more than 60%); Mix bacterium agent fermentation each bacterial classification ratio of back and all satisfactory purpose of activity: according to the Control essentials of present technique; three kinds of compositions can meet the requirements of ratio fully after the fermentation ends; and three kinds of bacterium adapted to mutually and keep separately activity and the ability of degradation of contaminant, for the large-scale production and the application of composite fungus agent are laid a good foundation.
(4) description of drawings
Fig. 1 is the growth curve of Phanerochaete chrysosporium in selected substratum;
Fig. 2 is the growth curve of Pseudomonas aeruginosa in selected substratum;
Fig. 3 is the growth curve of coral promise cassette bacterium in selected substratum;
Fig. 4 is a microbial biomass growth curve in the mixed strains process of high-density fermentation.
(5) embodiment
The present invention is described further below in conjunction with specific embodiment, but protection scope of the present invention is not limited in this:
Embodiment 1:
1. the activation of bacterial classification
(1) activation of Phanerochaete chrysosporium ATCC 20696: will be kept at-80 ℃ of bacterial classifications in the refrigerator and take out, melt on ice, aseptic technique is inoculated on the plate culture medium, 30 ℃ of constant temperature culture 48～72h, when treating that single bacterium colony appearance and size are fit to the picking inoculation, after sealing film phonograph seal, 4 ℃ of refrigerator preservations are standby.Plate culture medium: wort extractive substance 30gL
-1, agar powder 15gL
-1, with regulating pH to 5.5 behind the deionized water dissolving, 121 ℃ of sterilization 25min, it is standby to pour in the plate cooling under the aseptic condition into.
(2) activation of pseudomonas aeruginosa ACCC 40100: will be kept at-80 ℃ of bacterial classifications in the refrigerator and take out, melt on ice, aseptic technique is inoculated on the plate culture medium, 30 ℃ of constant temperature culture 24～36h, when treating that single bacterium colony appearance and size are fit to the picking inoculation, after sealing film phonograph seal, 4 ℃ of refrigerator preservations are standby.Beef extract-peptone nutrient agar: extractum carnis 3gL
-1, peptone 10gL
-1, sodium-chlor 5gL
-1, agar powder 15gL
-1, with regulating pH to 7.0 behind the deionized water dissolving, 121 ℃ of sterilization 25min, it is standby to pour in the plate cooling under the aseptic condition into.
(3) activation of coral promise cassette bacterium CGMCC No.1.239: will be kept at-80 ℃ of bacterial classifications in the refrigerator and take out, melt on ice, aseptic technique is inoculated on the plate culture medium, 30 ℃ of constant temperature culture 48～72h, when treating that single bacterium colony appearance and size are fit to the picking inoculation, after sealing film phonograph seal, 4 ℃ of refrigerator preservations are standby.GY substratum: glucose 10gL
-1, yeast extract paste 10gL
-1, agar powder 15gL
-1, regulate pH to 7.0 behind the deionized water dissolving, 121 ℃ of sterilization 25min, it is standby to pour in the plate cooling under the aseptic condition into.
2. the preparation of seed liquor
(1) preparation of Phanerochaete chrysosporium seed liquor: get the dull and stereotyped bacterial classification of cultivating of refrigerator preservation, aseptic technique picking list colony inoculation in the 500ml triangular flask that the 200ml malt extract medium is housed, 30 ℃, 100rpm constant temperature culture 24～36h.The corresponding substratum that the seed liquor substratum uses when being above-mentioned actication of culture does not add agar powder.
(2) preparation of pseudomonas aeruginosa seed liquor: get the dull and stereotyped bacterial classification of cultivating of refrigerator preservation, aseptic technique picking list colony inoculation in the 500ml triangular flask that the 200ml beef-protein medium is housed, 30 ℃, 180rpm constant temperature culture 24～36h.The corresponding substratum that the seed liquor substratum uses when being above-mentioned actication of culture does not add agar powder.
(3) preparation of coral promise cassette bacterium seed liquor: get the dull and stereotyped bacterial classification of cultivating of refrigerator preservation, aseptic technique picking list colony inoculation in the 500ml triangular flask that 200ml GY substratum is housed, 30 ℃, 100rpm constant temperature culture 24～36h.The corresponding substratum that the seed liquor substratum uses when being above-mentioned actication of culture does not add agar powder.
3. go up a jar fermentation
(1) go up a jar preparation work:
1. sterilization filter in the fermentor tank blow and vent system and corresponding flexible pipe are pulled down, wrapped up the back together with the feed supplement bottle that supplemented medium is housed with as standby behind 120 ℃ of sterilizations of the empty bottle of adorning pH regulator liquid 25min with gauze and kraft paper.The supplemented medium composition: carbon source is that massfraction is 60% glucose solution.
2. 6.5L is dissolved good basic medium and put into the 10L fermentor tank, 121 ℃ of sterilization 25min.Basic medium: glucose 10gL
-1, soy peptone 10gL
-1, SODIUM PHOSPHATE, MONOBASIC 10gL
-1, vitamins B
13mgL
-1, vitamin H 0.4mgL
-1, the trace element (400 *) 2.5mLL
-1, fermentation is with defoamer (the efficient polyether antifoam agent of PPE, Zhejiang University chemical plant) 0.1mLL
-1, regulate pH to 5.5 with phosphoric acid
3. after treating substratum cooling, load onto sterilization filter and corresponding flexible pipe, feed sterile air and stir 5min with the rotating speed of 180rpm.Stop ventilation, the dissolved oxygen levels that be set this moment is 100%.
4. under the aseptic condition, ammoniacal liquor is packed in the good empty bottle of sterilization, and itself and feed supplement bottle are connected to fermentor tank well (peristaltic pump links to each other with on-line detecting system, and can regulate application of sample speed automatically according to the detection data) through flexible pipe by peristaltic pump respectively.
(2) fermenting process
During the fermentation, temperature whole process is controlled at 30 ℃; The The Control of Dissolved Oxygen target is more than 30%, and when dissolved oxygen was not enough, first selection was to improve stirring velocity.When speed reaches 300rpm and can't satisfy the dissolved oxygen needs, begin to feed aseptic compressed air, stirring rake speed falls after rise to the 150rpm; Along with thalline increases the aerobic rising caused, can increase dissolved oxygen by strengthening air flow, when oxygen requirement further increases, can consider to feed aseptic pure oxygen and compressed-air actuated mixed gas increases dissolved oxygen levels.The vapor pipe of fermentor tank has unidirectional stop valve, a little higher than normal atmosphere of air pressure at control fermentor tank top, and prevent the air admission fermentor tank of outside.
1. Phanerochaete chrysosporium seed liquor 140ml aseptic technique is inoculated in the fermentor tank, control pH is that 150rpm begins fermentation at 5.5 ± 0.3 level (the fermentor tank sequence of control is set, is determined the interpolation speed of ammoniacal liquor by the peristaltic pump of on-line detecting system control), 30 ℃ of temperature, rotating speed of agitator.
2. behind the fermentation beginning 24h, aseptic technique is inoculated coral promise cassette bacterium by same inoculum size again, regulates pH to 7.0 ± 0.3 before the inoculation, continues fermentation 24h.
3. last aseptic technique inoculation pseudomonas aeruginosa seed liquor 140ml begins feed supplement after continuing to cultivate 24h.The feed supplement mode: three kinds of microorganisms of reference enter the opportunity of logarithmic phase, add according to the speed of exponential form, and the feed supplement time that the feed supplement amount of 1L is set is 36h, after feed supplement finishes, continue fermentation 2h, the end fermenting process.
4. close vent valve,, fermented liquid is discharged from discharge port by the pressure that ventilation produces.
5. after getting the fermented liquid dilution certain multiple of mixing, microscopy, the quantity of observing three kinds of bacterium is: 43 of Phanerochaete chrysosporiums, 139 of pseudomonas aeruginosas, 23 of coral promise cassette bacterium confirm that each bacterial classification ratio is in span of control.
6. after the fermentation ends, get the 10ml fermented liquid and put into weighing bottle, be dried to constant weight and its quality of weighing in 100 ℃ of baking ovens, obtain after the calculating that dry cell weight reaches 22.46gL in the fermented liquid
-1
Embodiment 2:
The difference of present embodiment and embodiment 1 is that the basic medium prescription is: glucose 15gL
-1, soy peptone 15gL
-1, SODIUM PHOSPHATE, MONOBASIC 8gL
-1, vitamins B
12mgL
-1, vitamin H 0.1mgL
-1, the trace element (400 *) 1mLL
-1, fermentation uses defoamer 0.1mLL
-1, regulate pH to 5.5 with phosphoric acid.Other steps and selected parameter are identical with embodiment 1, and fermentation ends obtains that dry cell weight reaches 24.7gL in the fermented liquid after as calculated
-1
Embodiment 3:
The difference of present embodiment and embodiment 1 is that the basic medium prescription is: glucose 10gL
-1, soy peptone 15gL
-1, SODIUM PHOSPHATE, MONOBASIC 12gL
-1, vitamins B
15mgL
-1, vitamin H 0.5mgL
-1, the trace element (400 *) 3mLL
-1, fermentation uses defoamer 0.1mLL
-1, regulate pH to 5.5 with phosphoric acid.Other steps and selected parameter are identical with embodiment 1, and fermentation ends obtains that dry cell weight reaches 23.1gL in the fermented liquid after as calculated
-1
Embodiment 4:
Use and biochemical section aeration tank, certain paper mill according to the composite fungus agent that above embodiment 1 produces: this sewage treatment process is day output 15000m
3/ D, influent quality (monthly average): chemical oxygen demand (COD) (COD) 3213mgL
-1, biological oxygen demand (BOD
5) 1200mgL
-1, suspended solids content (SS) 105mgL
-1Use the preceding effluent quality (monthly average) of this composite fungus agent: chemical oxygen demand (COD) (COD) 100mgL
-1, biological oxygen demand (BOD
5) 45mgL
-1, suspended solids content (SS) 20mgL
-1Effluent quality (monthly average) behind the use composite fungus agent: chemical oxygen demand (COD) (COD) 60mgL
-1, biological oxygen demand (BOD
5) 20mgL
-1, suspended solids content (SS) 17mgL
-1, as seen the composite fungus agent that cultivate to obtain by the inventive method to the gain of papermaking wastewater treatment effect clearly.
Claims (5)
1. a papermaking wastewater is handled the high-density cultivation method of using composite fungus agent, described method comprises: inoculate Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC 20696, coral promise cassette bacterium (Nocardia coralline) ACCC 40100 successively in basic medium and Pseudomonas aeruginosa (Pseudomonas aeruginosa) CGMCC No.1.239 carries out fermentation culture at high density fermentation, controlled temperature is that 28～32 ℃, pH5.0～7.5, dissolved oxygen are 1～5mgL
-1, in the fermenting process in batches stream to add cumulative volume be high density fermentation with the glucose solution of the mass concentration 50～60% of basic medium volume 10～20%, fermentation total time is 80～100h; Described high density fermentation is composed as follows with basic medium: glucose 10～15gL
-1, soy peptone 10～15gL
-1, SODIUM PHOSPHATE, MONOBASIC 8～12gL
-1, vitamins B
12～5mgL
-1, vitamin H 0.1～0.5mgL
-1, micro-mother liquor 1～3mLL
-1, fermentation uses defoamer 0.1mL.L
-1, regulate pH to 5.5 with phosphoric acid; Described micro-mother liquor is composed as follows: C
OCl
26H
2O1mgml
-1, MnCl
24H
2O 6mgml
-1, CuCl
22H
2O 0.6mgml
-1, H
3BO
41.2mgml
-1, Na
2M
oO
42H
2O 1mgml
-1, Zn (CH
3COO)
22H
2O 5.2mgml
-1, FeC
6H
5O
75H
2O 40mgml
-1
2. the method for claim 1 is characterized in that: cultivate 24h behind the inoculation Phanerochaete chrysosporium, inoculate coral promise cassette bacterium, inoculate Pseudomonas aeruginosa after continuing to cultivate 24h.
3. the method for claim 1 is characterized in that: described glucose solution begins stream and adds behind inoculation Pseudomonas aeruginosa 24h, and stream adds and finishes in 36h.
4. the method for claim 1 is characterized in that: control pH inoculates behind the coral promise cassette bacterium until fermentation ends control pH 7.0 ± 0.3 5.5 ± 0.3 behind the yellow archespore hair lead fungi of inoculation.
5. the method for claim 1 is characterized in that: described high density fermentation is composed as follows with basic medium: glucose 10gL
-1, soy peptone 10gL
-1, SODIUM PHOSPHATE, MONOBASIC 10gL
-1, vitamins B
13mgL
-1, vitamin H 0.4mgL
-1, micro-mother liquor 2.5mLL
-1, fermentation uses defoamer 0.1mLL
-1, regulate pH to 5.5 with phosphoric acid.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102643748A (en) * | 2012-05-09 | 2012-08-22 | 刘建伦 | Sludge-reduced microorganism combined dominant population in sewage processing |
| CN103159329A (en) * | 2013-04-02 | 2013-06-19 | 江苏艾特克环境工程有限公司 | Method for in-situ enhancement of microbial activity |
| CN104419643A (en) * | 2013-09-03 | 2015-03-18 | 大连大孤山污水处理有限公司 | Culture process of novel biological flora |
| CN105368748A (en) * | 2015-12-04 | 2016-03-02 | 无锡拓能自动化科技有限公司 | Urban sewage treatment compound bacteria and preparation method thereof |
| CN107164276A (en) * | 2017-06-21 | 2017-09-15 | 北京大学 | One plant tolerance zinc ion toxicity pseudomonas aeruginosa and its application |
| CN107227282A (en) * | 2017-08-08 | 2017-10-03 | 苏州克莱尔环保科技有限公司 | A kind of extraordinary flora of sewage disposal and its cultural method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1699220A (en) * | 2005-05-23 | 2005-11-23 | 南京工业大学 | Method and apparatus for immobilizing cells to treat wastewater |
| CN101973795A (en) * | 2010-10-21 | 2011-02-16 | 哈尔滨工业大学 | Sludge aerobic composting method by utilizing composting compound microbial inoculum |
-
2011
- 2011-07-01 CN CN 201110183629 patent/CN102250805B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1699220A (en) * | 2005-05-23 | 2005-11-23 | 南京工业大学 | Method and apparatus for immobilizing cells to treat wastewater |
| CN101973795A (en) * | 2010-10-21 | 2011-02-16 | 哈尔滨工业大学 | Sludge aerobic composting method by utilizing composting compound microbial inoculum |
Non-Patent Citations (2)
| Title |
|---|
| 《WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY》 20081231 Peng Wang "Effect of culture conditions on the production of ligninolytic enzymes by white rot fungi Phanerochaete chrysosporium (ATCC 20696) and separation of its lignin peroxidase." 1-5 第24卷, 第10期 * |
| 《中国优秀硕士学位论文全文数据库（工程科技Ⅰ辑）》 20100615 于鹏 "造纸废水生物强化活性炭脱色深度处理研究" 1-5 , 第6期 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102643748A (en) * | 2012-05-09 | 2012-08-22 | 刘建伦 | Sludge-reduced microorganism combined dominant population in sewage processing |
| CN103159329A (en) * | 2013-04-02 | 2013-06-19 | 江苏艾特克环境工程有限公司 | Method for in-situ enhancement of microbial activity |
| CN103159329B (en) * | 2013-04-02 | 2014-12-10 | 江苏艾特克环境工程有限公司 | Method for in-situ enhancement of microbial activity |
| CN104419643A (en) * | 2013-09-03 | 2015-03-18 | 大连大孤山污水处理有限公司 | Culture process of novel biological flora |
| CN105368748A (en) * | 2015-12-04 | 2016-03-02 | 无锡拓能自动化科技有限公司 | Urban sewage treatment compound bacteria and preparation method thereof |
| CN107164276A (en) * | 2017-06-21 | 2017-09-15 | 北京大学 | One plant tolerance zinc ion toxicity pseudomonas aeruginosa and its application |
| CN107164276B (en) * | 2017-06-21 | 2020-08-04 | 北京大学 | Zinc ion toxicity-resistant pseudomonas aeruginosa and application thereof |
| CN107227282A (en) * | 2017-08-08 | 2017-10-03 | 苏州克莱尔环保科技有限公司 | A kind of extraordinary flora of sewage disposal and its cultural method |
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