CN106399200B - Alcaligenes and application thereof in high-salt high-polymer wastewater - Google Patents
Alcaligenes and application thereof in high-salt high-polymer wastewater Download PDFInfo
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- CN106399200B CN106399200B CN201610979936.2A CN201610979936A CN106399200B CN 106399200 B CN106399200 B CN 106399200B CN 201610979936 A CN201610979936 A CN 201610979936A CN 106399200 B CN106399200 B CN 106399200B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/05—Alcaligenes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Abstract
The invention discloses an alcaligenes and application thereof in high-salinity high-polymer wastewater. The preservation number of the Alcaligenes sp Z-1 is CGMCC No. 12585. The invention can effectively decompose macromolecular polymers such as polyacrylamide, polysulfonate substances and the like into micromolecular easily-degradable substances in the environment of high-salinity high-polymer wastewater, and obtains energy by decomposing organic matters to maintain the growth and the propagation of the polymer. In the high-salt high-polymer wastewater containing polyacrylamide, polysulfones and other organic matters treated by the bacteria, the decomposition rate of high polymers can reach more than 95 percent, the COD of the wastewater can be effectively reduced, and the removal rate of the COD can reach more than 50 percent.
Description
Technical Field
The invention belongs to the field of microorganism and wastewater treatment, and relates to an alcaligenes Z-1 and application thereof in high-salt high-polymer wastewater.
Background
A large amount of high-salt degradation-resistant organic wastewater is produced in the petroleum and chemical industry of China every year, the organic wastewater often contains a large amount of artificially synthesized macromolecular polymer additives, and the polymers have large molecular weight and complicated structure, are difficult to degrade, exist in the environment for a long time and have great harm to the environment and people. The direct discharge of such wastewater causes serious pollution to surface water, underground water, soil, plants and ecological environment, causes many negative impacts on the survival and development of enterprises, and also becomes a problem of high concern to society, public and government departments.
Zhang Shuman, Hojie, etc. found the microbial composite bacterial agent HBS5 in the technical research on composite bacterial agent for treating deep well polysulfonate drilling fluid wastewater, and the optimal conditions for treating the difficult degradable polysulfonate drilling fluid wastewater are as follows: the temperature is above 20 ℃, the adding amount of the microbial inoculum is 0.5g/L (25 to 35 percent of SV 30), the aeration amount is 0.25L/m in, and the COD mass concentration of the initial drilling wastewater is less than or equal to 1100 mg/L.
although many reports are made on the treatment of wastewater containing macromolecular organic matters such as sulfospecies and polyacrylamide at home and abroad, the effect of treating refractory wastewater with microorganisms with the conductance of 15000-38000 us/cm and the COD of 3500-7000 mg/L is not ideal, so that one or more strains which can survive in high-salt wastewater with the conductance of 15000-38000 us/cm and can efficiently degrade sulfospecies and polyacrylamide are found.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a strain capable of effectively decomposing macromolecular polymers such as polyacrylamide, polysulfonates and the like in a high-salt high-polymerization environment.
The invention is realized by the following technical scheme:
The invention provides a strain Z-1 which is identified as Alcaligenes sp by molecular biology, and the strain is preserved in China general microbiological culture Collection center (CGMCC for short) for 2016-6-2 days at the address of No. 3 of No.1 Hopkin, West Lu of Beijing Korean district, No. 3 of the institute of microbiology, China academy of sciences, and the preservation registration number is CGMCC NO. 12585.
The invention also provides application of the Alcaligenes sp Z-1 in high-salt high-polymer wastewater treatment, wherein the dissolved oxygen amount in the high-salt wastewater is 2.0-7.0 mg/L.
In the application of the invention, the inorganic salts in the high-salt high-polymer wastewater are NaCl, CaCl 2 and MgCl 2, the conductance is 15000-38000 us/cm, the COD of the high-salt wastewater is 5000-7000 mg/L, and the COD provided by the polymer accounts for 1-20% of the total COD.
And adding a growth promoter into the high-salt high-COD wastewater, wherein the production promoter is selected from one or more of monosaccharide, disaccharide and polysaccharide. Preferably, the COD provided by the addition amount of the growth promoter is 5 per mill-5% of the COD of the original high-salinity high-COD wastewater.
the Alcaligenes (Alcaligenes sp.) Z-1 is added into high-salinity high-polymer wastewater, and the dosage of the composite biological bacteria/the amount of the organic wastewater is preferably 0.1-2 wt%. After a certain period of aeration, the organic matters in the wastewater can be degraded, and the COD of the wastewater is reduced.
Compared with the prior art, the alcaligenes Z-1 provided by the invention can effectively decompose macromolecular organic matters into micromolecular substances which are easy to degrade, obtains energy by decomposing the organic matters, and maintains the growth and the propagation of the alcaligenes by utilizing various micromolecular organic matters such as monosaccharide, micromolecular organic acid or alcohol in wastewater. When the alkaligenic bacteria Z-1 are used for treating high-salinity wastewater containing organic matters such as polyacrylamide and polysulfones, the decomposition rate of high polymers can reach more than 95 percent, the COD of the wastewater can be effectively reduced, and the removal rate of the COD can reach more than 50 percent. The alcaligenes Z-1 is adopted to treat high-salinity high-polymer wastewater, so that the wastewater treatment cost can be greatly reduced, and the energy consumption and material consumption of the wastewater treatment can be reduced; and along with the increase of the adding amount of the strain alcaligenes Z-1, the degradation efficiency is greatly improved, thereby improving the biochemical efficiency and reducing the biochemical occupied area. In addition, after the biochemical system is impacted, the strain Z-1 is added, so that the recovery time of the biochemical system can be shortened.
Detailed Description
The technical solution of the present invention is further described in detail with reference to examples below. The following examples are intended to illustrate the invention only and do not limit the scope of the invention.
EXAMPLE 1 preparation of microbial Agents and use in waste Water of Polyacrylamide production plants
The strain Z-1 is obtained from the bottom mud of a drilling wastewater storage pool through domestication culture, screening and separation.
Biological characterization of Z-1
Z-1 is a single coccus, gram-negative bacterium. On a solid culture medium, the bacterial colony is round, white, smooth, moist, glossy, neat in edge and non-sticky.
2. The identification of the strain Z-116S rDNA comprises the following specific steps:
Forward primer F1: 5'-AGAGTTTGATCCTGGCTCAG-3', reverse primer R1: 5'-GGTTACCTTGTTACGACTT-3', DNA amplification (PCR) was performed. And (3) PCR reaction conditions: pre-denaturation at 95 deg.C for 5min, denaturation at 95 deg.C for 30s, annealing at 58 deg.C for 30s, extension at 72 deg.C for 90s, 35 cycles, final extension at 72 deg.C for 10min, and storage at 4 deg.C. The PCR product was separated on 0.8% agarose gel, purified and recovered for sequencing. The sequencing used NCBIblast alignment analysis to determine that strain Z-1 is subordinate to Alcaligenes sp.
The 16S rDNA sequence of the Alcaligenes sp Z-1 is shown in a detailed sequence table, the strain is preserved in the common microorganism center of China Committee for culture Collection of microorganisms at 2.6.2016, the address is No. 3 of No.1 Hospital of West Chen of the Korean district in Beijing, and the registration number is CGMCC NO. 12585.
3. The method for culturing the alcaligenes Z-1 strain comprises the following steps:
1) Preparation of seed liquid
A. Solid medium: beef extract: 10g, peptone: 10g, NaCl: 5g, glucose: 10g, distilled water: 1000ml, agar powder: 18g, pH: 7.0-7.2;
B. Liquid culture medium: beef extract: 10g, peptone: 10g, NaCl: 5g, glucose: 10g, distilled water: 1000ml, pH: 7.0-7.2;
C. Sterilizing the culture medium with high temperature steam (121 deg.C, 30min), pouring onto sterilized flat plate in super clean bench, and solidifying the culture medium;
D. Inoculating the bacteria Z-1 on the flat plate by a streaking method, and inversely placing the flat plate in an incubator at 30 ℃ for culturing for 24-36 h until bacterial colonies grow out;
E. Scraping off a well-grown Z-1 colony on an inoculating loop taking flat plate, inoculating the colony in a liquid culture medium, wherein the liquid filling amount of a triangular flask is 60%, and culturing for 24-36 h at the temperature of 30 ℃, the rpm of 120 and the logarithmic phase to obtain a seed solution.
2) Fermenting in a fermentation tank
A. The fermentation liquid culture medium comprises 15g/L glucose, KH 2 PO 4 5g/L, (NH 4) 2 HPO 4 6g/L, 0.9g/L citric acid, 19.5g/L yeast extract, 5mL trace element solution, 0.1% antifoaming agent, 1000mL distilled water, and pH adjusted to 7.0. the trace element solution comprises FeSO 4 & 7H 2 O10 g/L, ZnSO 4 & 7H 2 O5.25 g/L, CuSO 4 & 5H 2 O3 g/L, H 3 BO 3 0.3g/L, CoCl 2.6H 2 O0.04 g, CaCl 2 2.0g/L, Na 2 MoO 4 & 2H 2 O0.15 g/L, 1000mL distilled water, pH7.0.
B. transferring the Z-1 seed solution obtained by the culture to a fermentation tank according to the inoculum size of 2-5% of the total amount of the fermentation liquid, and carrying out fermentation culture under the conditions of the tank temperature of 30 ℃, the tank pressure of 0.03MPa, the ventilation volume of 1:1V/V/min, the stirring speed of 150rpm and the fermentation time of 12-18 h, so that the bacteria concentration in the fermentation liquid is 3 multiplied by 10 9 CFU/mL;
3) preservation of microbial inoculum
And standing and precipitating the obtained zymophyte liquid, concentrating by 3-5 times to prepare a liquid microbial inoculum, and storing in an environment at 4 ℃.
4) Use of microbial Agents
Adding the high-efficiency composite biological agent into tap water for aeration and activation for 0.5-1h during the use period, wherein the adding amount of the agent is as follows: the amount of the bacteria/water is 0.1-2 per mill, and the bacteria/water can be added once or for multiple times.
Treatment of wastewater from a factory producing polyacrylamide with Z-1 bacteria
the conductance of the wastewater in a certain factory for producing polyacrylamide is 45000-60000 us/cm, the inorganic salt is mainly NaCl, and the COD is 12000-18000 mg/L, wherein the COD content of polyacrylamide accounts for 15% -18% of the total amount. Diluting the wastewater by 2-3 times, and treating the wastewater by using an efficient strain Z-1. The water inlet conductance is 16000-25000 us/cm, the COD is 5500-6000 mg/L, the COD content provided by polyacrylamide accounts for 5-6% of the total amount, the adding amount of Z-1 bacteria is 1 per mill, the COD of the effluent reaches below 1500mg/L after aerobic treatment for 24-36 h, and the COD content provided by polyacrylamide accounts for 0.3-0.5% of the total COD of the effluent.
example 2: z-1 bacteria treatment for treating drilling wastewater of certain offshore platform
Different from the embodiment 1, COD in the drilling wastewater of a certain offshore platform is 5000-7000 mg/L, wherein the polyacrylamide provides COD which accounts for about 5% of the total COD, the polysulfonate macromolecular substances provide COD which accounts for about 6-10% of the total COD, and the conductivity of the wastewater is 27000-35000 us/cm. The wastewater is treated by Z-1 bacteria, the adding amount of the bacteria is 2 per mill, wherein after aerobic treatment for 48 hours, the COD of effluent reaches below 1500 mg/L; and adding a growth promoter while adding the strain, wherein the COD provided by the growth promoter is about 200mg/L, the COD of the effluent is less than 500mg/L, and the contents of polyacrylamide and polysulfonates are both less than 0.2%.
example 3: z-1 bacterium treatment of natural gas drilling wastewater
The difference from the embodiment 1 is that the treated wastewater is natural gas drilling wastewater in certain places in Shanxi, the COD of the effluent is 5500mg/L, the conductance is 25000-28000 us/cm, wherein the macromolecules such as polysulfones and the like account for about 8%, 0.5 thousandth of Z-1 bacteria are added for treatment, the COD of the effluent reaches 500mg/L after 48 hours, and the proportion of the macromolecules such as polysulfones and the like is below 0.3%.
Principle of the invention
The alcaligenes Z-1 is a bacterium which can degrade macromolecular organic matters such as polyacrylamide, polysulfones and the like and is separated and screened from bottom mud of a drilling wastewater storage pool. In a high-salinity wastewater living environment with the electric conductivity of 15000-38000 us/cm, firstly, generating a biological hydrolase with a certain degradation capability on a polymer, firstly, attacking a side chain of the polymer, carrying out chain-removing decomposition on the side chain, and maintaining and providing self growth needs by utilizing a carbon source, a nitrogen source and a sulfur source generated in the decomposition process. After the side chain is decomposed, the main chain is exposed and then outside, the main chain of the polymer is gradually broken under the action of monooxygenase secreted by Z-1, and the polymer is broken into substances with smaller molecular weight, so that the microorganism can utilize the polymer macromolecule as a carbon source. The polyacrylamide with long chain is broken into short chain through a series of oxidation reactions in which the multiple microbial enzymes participate, and the short chain is further utilized by the microbes to generate acetic acid, so that macromolecular polymers such as polyacrylamide, polysulfones and the like are utilized by the microbes, and the aim of degrading the COD in the wastewater is fulfilled in the process that the microbes utilize organic matters to maintain self growth and propagation.
SEQUENCE LISTING
<110> Zhonghai oil Tianjin chemical research design institute Co., Ltd
Zhonghai oil energy development Co., Ltd
<120> alcaligenes and application thereof in high-salt high-COD wastewater
<130> THY-16-63
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1387
<212> DNA
<213> Alcaligenes sp.
<400> 1
gagagagctt gctctcttgg tggcgagtgg cggacgggtg agtaatatat cggaacgtgc 60
ccagtagcgg gggataacta ctcgaaagag tggctaatac cgcatacgcc ctacggggga 120
aaggggggga ttcttcggaa cctctcacta ttggagcggc cgatatcgga ttagctagtt 180
ggtggggtaa aggctcacca aggcaacgat ccgtagctgg tttgagagga cgaccagcca 240
cactgggact gagacacggc ccagactcct acgggaggca gcagtgggga attttggaca 300
atgggggaaa ccctgatcca gccatcccgc gtgtatgatg aaggccttcg ggttgtaaag 360
tacttttggc agagaagaaa aggtatctcc taatacgaga tactgctgac ggtatctgca 420
gaataagcac cggctaacta cgtgccagca gccgcggtaa tacgtagggt gcaagcgtta 480
atcggaatta ctgggcgtaa agcgtgtgta ggcggttcgg aaagaaagat gtgaaatccc 540
agggctcaac cttggaactg catttttaac tgccgagcta gagtatgtca gaggggggta 600
gaattccacg tgtagcagtg aaatgcgtag atatgtggag gaataccgat ggcgaaggca 660
gccccctggg ataatactga cgctcagaca cgaaagcgtg gggagcaaac aggattagat 720
accctggtag tccacgccct aaacgatgtc aactagctgt tggggccgtt aggccttagt 780
agcgcagcta acgcgtgaag ttgaccgcct ggggagtacg gtcgcaagat taaaactcaa 840
aggaattgac ggggacccgc acaagcggtg gatgatgtgg attaattcga tgcaacgcga 900
aaaaccttac ctacccttga catgtctgga atcccgaaga gatttgggag tgctcgcaag 960
agaaccggaa cacaggtgct gcatggctgt cgtcagctcg tgtcgtgaga tgttgggtta 1020
agtcccgcaa cgagcgcaac ccttgtcatt agttgctacg caagagcact ctaatgagac 1080
tgccggtgac aaaccggagg aaggtgggga tgacgtcaag tcctcatggc ccttatgggt 1140
agggcttcac acgtcataca atggtcggga cagagggtcg ccaacccgcg agggggagcc 1200
aatctcagaa acccgatcgt agtccggatc gcagtctgca actcgactgc gtgaagtcgg 1260
aatcgctagt aatcgcggat cagaatgtcg cggtgaatac gttcccgggt cttgtacaca 1320
ccgcccgtca caccatggga gtgggttcac cagaagtagg tagcctaacc gaaggaggcg 1380
ctaccac 1387
Claims (5)
1. An Alcaligenes (Alcaligenes sp.) Z-1 is characterized in that the preservation number of the Alcaligenes Z-1 is CGMCC No. 12585.
2. The application of the Alcaligenes sp Z-1 in the treatment of high-salt high-polymer wastewater is characterized in that the dissolved oxygen amount in the high-salt high-polymer wastewater is 2.0-7.0 mg/L, inorganic salts in the high-salt high-polymer wastewater are NaCl, CaCl 2 and MgCl 2, the electric conductivity is 15000-38000 us/cm, the COD is 5000-7000 mg/L, the polymer is polyacrylamide and polysulfonate polymer, and the COD provided by the polymer accounts for 1% -20% of the total COD.
3. The application of the method as claimed in claim 2, wherein a growth promoter is added into the high-salinity high-polymer wastewater, and the growth promoter is selected from one or more of monosaccharide, disaccharide and polysaccharide.
4. The use according to claim 3, wherein the amount of the growth promoter added provides COD 5% o to 5% of the COD of the original high salinity wastewater.
5. The use according to claim 2, wherein the addition amount of the alcaligenes Z-1 is 0.1 to 2 wt% of the high salinity and high polymer wastewater.
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| CN109650557B (en) * | 2019-02-14 | 2021-10-08 | 上海海事大学 | Method for reducing content of calcium and magnesium ions in seawater, microbial scale inhibitor and application |
| KR20230169409A (en) * | 2021-05-28 | 2023-12-15 | 킴벌리-클라크 월드와이드, 인크. | Optimization of halophilic PHB depolymerase for industrial applications |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101407769A (en) * | 2007-10-11 | 2009-04-15 | 同济大学 | Dry powder biological demulsifying agent and Bacillus alcaligenes applied to petroleum extraction |
| CN103013894A (en) * | 2013-01-21 | 2013-04-03 | 东北农业大学 | Lead-tolerant alcaligenes sp. BAPb.1 and application of same |
| CN105923900A (en) * | 2016-05-18 | 2016-09-07 | 杭州富阳高博信息技术服务有限公司 | Sewage treatment method used in the urban green space planning |
| CN106011009A (en) * | 2016-06-15 | 2016-10-12 | 中国科学院生态环境研究中心 | Alcaligenes sp. GTM2 and application thereof to chromium-containing wastewater treatment |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH062059B2 (en) * | 1988-12-13 | 1994-01-12 | 学校法人金井学園 | Method for producing chitosanase |
-
2016
- 2016-11-08 CN CN201610979936.2A patent/CN106399200B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101407769A (en) * | 2007-10-11 | 2009-04-15 | 同济大学 | Dry powder biological demulsifying agent and Bacillus alcaligenes applied to petroleum extraction |
| CN103013894A (en) * | 2013-01-21 | 2013-04-03 | 东北农业大学 | Lead-tolerant alcaligenes sp. BAPb.1 and application of same |
| CN105923900A (en) * | 2016-05-18 | 2016-09-07 | 杭州富阳高博信息技术服务有限公司 | Sewage treatment method used in the urban green space planning |
| CN106011009A (en) * | 2016-06-15 | 2016-10-12 | 中国科学院生态环境研究中心 | Alcaligenes sp. GTM2 and application thereof to chromium-containing wastewater treatment |
Non-Patent Citations (4)
| Title |
|---|
| Maintenance and Induction of Naphthalene Degradation Activity in Pseudomonas putida and an Alcaligenes sp. under Different Culture Conditions;WILLIAM F. GUERIN et al.;《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》;19951130;第61卷(第11期);全文 * |
| 一株聚丙烯酰胺降解菌的分离鉴定及其生物降解;魏利 等;《华东理工大学学报：自然科学版》;20071231(第1期);全文 * |
| 一株聚丙烯酰胺降解菌降解聚丙烯酰胺及原油性能研究;马少华 等;《环境科学学报》;20041130;第24卷(第6期);全文 * |
| 微生物降解直链十二烷基苯磺酸钠的研究;刘秀荣 等;《北京工业大学学报》;19951231;第21卷(第4期);全文 * |
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