CN108085276A - For breeding pollution and the micro-ecological complex bacterial agent as enteron aisle improver and its application simultaneously - Google Patents
For breeding pollution and the micro-ecological complex bacterial agent as enteron aisle improver and its application simultaneously Download PDFInfo
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- CN108085276A CN108085276A CN201711433290.9A CN201711433290A CN108085276A CN 108085276 A CN108085276 A CN 108085276A CN 201711433290 A CN201711433290 A CN 201711433290A CN 108085276 A CN108085276 A CN 108085276A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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Abstract
The invention discloses for breeding pollution and the micro-ecological complex bacterial agent as enteron aisle improver and its application simultaneously, the composite bacteria agent are combined in proportion by Bacillus foecalis alkaligenes, bacillus, Bifidobacterium, Bacillus acidi lactici, saccharomycete.The bacillus includes but not limited to bacillus subtilis, bacillus stearothermophilus, bacillus licheniformis, bacillus amyloliquefaciens, bacillus pumilus etc..Mass percent of each microorganism in microbial total quality is in the composite bacteria agent：Bacillus foecalis alkaligenes 5 ~ 15%, bacillus 10 ~ 20%, Bifidobacterium 25 ~ 45%, Bacillus acidi lactici 12 ~ 46%, saccharomycete 8 ~ 20%.The micro-ecological complex bacterial agent can be established and recover the dominant microflora ecological balance in enteron aisle, inhibit the growth of spoilage organisms in enteron aisle, reduce conversion of the protein to amine and ammonia, decline the content of ammonia and amine in enteral and blood, reduce the pernicious gases such as the ammonia excreted with excrement, air quality in house is improved, reduces environmental pollution.
Description
Technical field
The invention belongs to complex microorganism preparations technical fields, and in particular to one kind is used as intestines for breeding pollution and simultaneously
The micro-ecological complex bacterial agent of road improver and its application.
Background technology
Since the 1990s, the development of large-scale cultivation is solving animal product supply and is driving rural economy
Also the problem of environmental pollution of getting worse is brought while development.Untreated feces of livestock and poultry is arbitrarily accumulated, sewage excrement
Arbitrarily discharge, so as to bring a series of environmental problem.Polluted by nitrogen problem is the most prominent wherein in livestock breeding wastewater, Duo Shuoyang
The ammonia nitrogen grown in waste water is all exceeded, and the nitrate content in underground water can be caused excessively high, equally easily causes the rich battalion of water body
Fosterization makes water quality deterioration.Nitrogen is to the problem of environmental pollution that the pollution of water body has not been a part, but one influences large watershed
The problem of environment, it endangers the eutrophic extent of river mouth, offshore sea waters, also serious to destroy entire ecological environment.Livestock and poultry
The problem of polluted by nitrogen problem is common concern both at home and abroad in waste water.At present, microbial method and object in terms of environmental pollution by water processing
Reason, chemical method compare, with its high efficiency, low cost, non-secondary pollution etc. it is incomparable the advantages of recognized extensively by people
Can, become environmental science and the main contents of water treatment field concern and research.
So-called complex microorganism preparations refer to by two or more microorganism through co-incubation, mutual between each other
Phase separation can reach the micropopulation for playing maximum function advantage.Complex microorganism preparations have the huge of reality and potentiality
Purposes has been widely used in the every field such as agricultural, industry, medicine and environmental protection, in field of environment protection, complex microorganism tool
Have quick, small investment, it is easy to operate, the advantages such as secondary pollution will not be caused, show its huge development prospect.
It is apparent that the usage amount of Environmental Protection in China microbial bacterial agent expands trend, according to incompletely statistics, microbial inoculum dosage in recent years
For average year speedup up to more than 15%, environmental protection microbial inoculum in domestic market mainly has 3 sources：One kind is external import, and share accounts for
More than the 60% of total amount represents producer as Novi of Denmark letter, Foshan Bi Wofeng（U.S. Bioform）, Japanese university of Ryukyu（EM
Bacterium）Deng；One kind produces for domestic enterprise, accounts for 30% of the market share or so, and representing producer has Guangzhou agriculture hat（Taiwan investment）Biological section
Skill Co., Ltd, Guang Yu Bioisystech Co., Ltd etc.；3rd is domestic universities' research institutes development and production, account for 5%~
10% share, main representative are the colleges and universities such as institute of microbiology of the Chinese Academy of Sciences, Tsinghua University.It specializes in environmental protection and uses microorganism in the country
Microbial inoculum production enterprise have 35 or so, wherein have more than 80% producer production microbial inoculum be by Japan introduce EM bacterium with
And based on the compounding of related microbial inoculum；There are 10% or so enterprise and domestic scientific research institutes to combine the exploitation for carrying out environmentally friendly special bacteria agent
With production.In terms of present situation, the enterprise that the country is engaged in the production of environmental protection microbial inoculum is more, but production scale of enterprise is smaller, and about 5~50
T/, the concentration degree of industry is low, and the microbial inoculum produced does not have import based on the EM bacterium of imitated Japan to the treatment effect of waste water
Microbial inoculum is targeted.The environmentally friendly special bacteria agent of domestic universities' research institutes research and development, effect can match in excellence or beauty with import microbial inoculum, but product turns
Change is slower, is also in the stage of marketing one's own products substantially, fails real formation scale.
The content of the invention
Breeding pollution and the simultaneously micro-ecological composite bacterium as enteron aisle improver are used for the object of the present invention is to provide a kind of
Agent and its application, the micro-ecological complex bacterial agent can be established and recover the dominant microflora ecological balance in enteron aisle, and profitable strain exists
Enteron aisle generates the antibacterial substances such as organic acid, hydrogen peroxide and bacteriocin, so as to inhibit the growth of spoilage organisms in enteron aisle, reduces
Urease activity, reduces conversion of the protein to amine and ammonia, declines the content of ammonia and amine in enteral and blood, so
Just reduce the pernicious gases such as the ammonia excreted with excrement, improve air quality in house, reduce environmental pollution.
To achieve these goals, the technical solution adopted by the present invention is：
It is a kind of for the breeding pollution and micro-ecological complex bacterial agent as enteron aisle improver, the composite bacteria agent produce alkali by excrement simultaneously
Bacillus, bacillus, Bifidobacterium, Bacillus acidi lactici, saccharomycete are combined in proportion.
The bacillus includes but not limited to bacillus subtilis, bacillus stearothermophilus, bacillus licheniformis, solution
Bacillus amyloliquefaciens, bacillus pumilus etc..
Mass percent of each microorganism in microbial total quality is in the composite bacteria agent：Bacillus foecalis alkaligenes 5 ~
15%th, bacillus 10 ~ 20%, Bifidobacterium 25 ~ 45%, Bacillus acidi lactici 12 ~ 46%, saccharomycete 8 ~ 20%.
It is further preferred that mass percent of each microorganism in microbial total quality is in the composite bacteria agent：
Bacillus foecalis alkaligenes 6%, bacillus 10%, Bifidobacterium 30%, Bacillus acidi lactici 34%, saccharomycete 20%.
It is further preferred that mass percent of each microorganism in microbial total quality is in the composite bacteria agent：
Bacillus foecalis alkaligenes 10%, bacillus 15%, Bifidobacterium 40%, Bacillus acidi lactici 25%, saccharomycete 10%.
It is further preferred that the bacillus is bacillus subtilis and bacillus licheniformis in mass ratio 1:1 mixing
It forms.
It is described for breeding pollution and the micro-ecological complex bacterial agent as enteron aisle improver is added as feed simultaneously
Agent or the application of breeding pollution renovation agent.
The pollution that complex microbial inoculum of the present invention mitigates odorant in fecaluria is to initially set up and recover dominant bacteria in enteron aisle
Group and microecological balance, since profitable strain is in antibacterial substances such as enteron aisle generation organic acid, hydrogen peroxide and bacteriocins, thus
The growth of spoilage organisms in enteron aisle is inhibited, reduces urease activity, reduces conversion of the protein to amine and ammonia, makes enteral
Decline with the content of ammonia in blood and amine, which reduces the pernicious gases such as the ammonia excreted with excrement, improve house
Interior air quality, reduces environmental pollution.
Complex microbial inoculum of the present invention can not only generate alkalescence, neutral or acid protease, amylase and lipase, together
When also generate the enzymes of complex carbohydrates in degradable plant feed.Probiotics by supplementing alimentary canal profitable strain,
Increase body endogenous enzymes, so as to directly participate in the metabolism of nitrogen substance, have notable work to the amount for reducing nitrogen substance in excrement
With.
Alcaligenes faecalis is added in compound bacteria of the present invention, which can colonize in the enteric cavity of animal, can inhibit golden yellow Portugal
The growth of the microorganisms such as grape coccus, Pseudomonas aeruginosa, proteus, thus feed addictive can be used as harmless to growth of animal,
Meanwhile the bacterial strain can also be applied in wastewater treatment, Bacillus foecalis alkaligenes has nitrogen phosphorus certain removal effect, available for cultivating
Pollution is handled, and has obvious effect to N, P pollution for reducing feces of livestock and poultry.
Add bacillus for example：Bacillus stearothermophilus, bacillus licheniformis, bacillus subtilis and short and small gemma
Bacillus etc. can not only generate alkalescence, neutral or acid protease, amylase and lipase, while also generate degradable plant feed
The enzyme of middle complex carbohydrates promotes vivo protein to absorb, is largely discharged from excrement so as to reduce nitrogen substance, reduces
Pollution.Protease, lipase, amylase can equally consume livestock and poultry and be discharged to external without digesting complete N, P, reduce and support
Grow pollution.
Addition Bifidobacterium and Bacillus acidi lactici can be established and recover the dominant microflora ecological balance in enteron aisle, profitable strain
The antibacterial substances such as organic acid, hydrogen peroxide and bacteriocin are generated in enteron aisle, so as to inhibit the growth of spoilage organisms in enteron aisle, drop
Low urease activity, reduces conversion of the protein to amine and ammonia, declines the content of ammonia and amine in enteral and blood, this
Sample just reduces the pernicious gases such as the ammonia excreted with excrement.
Bifidobacterium can prevent decarboxylation of the pathogenic bacteria to amino acid, reduce the concentration of intestinal contents amine and hydrogen sulfide,
And then the odorant discharged with excrement is reduced, it preserves the ecological environment.
Addition saccharomycete can reduce the concentration of ammoniacal nitrogen in cud, improve non-ammoniacal nitrogen in duodenum and be particularly micro- life
The concentration of object nitrogen, and 12 fat intestines amino acid also change, the wherein content of methionine, sarcosine, serine and threonine
It improves.
Heretofore described Bacillus foecalis alkaligenes, bacillus, Bifidobacterium, the bacterial strains such as Bacillus acidi lactici, saccharomycete are this
Common bacterial strain in field feed addictive and pollution processing.
Advantageous effect：
The micro-ecological complex bacterial agent of the present invention can be used for breeding pollution to handle simultaneously as enteron aisle improver, can establish and recover
The dominant microflora ecological balance in enteron aisle as feed addictive, can promote to cultivate while as animal intestinal tract improver
The processing of pollution, pig farm is using after composite bacteria agent of the present invention, and the pig house ammonia removal rate of dry cleaning up excrement structure is 87.2%, pig manure
Just N, P contaminant capacity reduce by more than 50 percent in pollutant.Complex microbial inoculum of the present invention is added in chicken feed, is removed
Smelly significant effect, except ammonia rate is up to 79.7%.
Specific embodiment
Bacterial strain described in following embodiment is commercial sources purchase gained, following to provide bacterial strain uses therefor in embodiment
Source and model only as the embodiment of the present invention is preferably explained, are not particularly limited bacterial strain.
The bacillus subtilis is specially bacillus subtilis 1.1086（In China General Microbiological culture presevation management
The heart）；Bacillus licheniformis is specially bacillus licheniformis（CICC20085）, purchased from CICC, (Chinese industrial microorganism fungus kind protects
Hide administrative center)；Bacillus amyloliquefaciens（CICC20164）Purchased from CICC (in the management of Chinese industrial Microbiological Culture Collection
The heart), Bacillus foecalis alkaligenes（BNCC186639）Biology is received purchased from north；Bifidobacterium is bifidobacterium coryneforme 1.2279（It is Chinese common
Microbiological Culture Collection administrative center）；Bacillus acidi lactici（BNCC106633）Biology is received purchased from north；Saccharomycete is specially Candida utilis
Yeast BNCC188871 receives biology purchased from north.The bacterial strain is cultivated under respective optimum condition obtains thalline, and alkali bar is produced according to excrement
Bacterium 5 ~ 15%, bacillus 10 ~ 20%, Bifidobacterium 25 ~ 45%, Bacillus acidi lactici 12 ~ 46%, the ratio of saccharomycete 8 ~ 20% are compound to be obtained
To the micro-ecological complex bacterial agent of the present invention.
Embodiment 1
It is a kind of for breeding pollution and the micro-ecological complex bacterial agent as enteron aisle improver simultaneously, each microorganism in composite bacteria agent
Mass percent in microbial total quality is：Bacillus foecalis alkaligenes 6%, bacillus 10%, Bifidobacterium 30%, Bacillus acidi lactici
34%th, saccharomycete 20%.Wherein described bacillus is bacillus subtilis and bacillus licheniformis in mass ratio 1:1 mixing and
Into.
By above-mentioned composite bacteria agent in mass ratio 1:50 and pannage（Honest feed）Mixing, for the nursing of pig, pig farm makes
After composite bacteria agent of the present invention, the pig house ammonia removal rate for doing cleaning up excrement structure is 87.2%, and N, P pollute in swine excrement pollutant
Amount reduces by 58.5% and 69.4%.
Embodiment 2
It is a kind of for breeding pollution and the micro-ecological complex bacterial agent as enteron aisle improver simultaneously, each microorganism in composite bacteria agent
Mass percent in microbial total quality is：Quality of each microorganism in microbial total quality in the composite bacteria agent
Percentage is：Bacillus foecalis alkaligenes 10%, bacillus 15%, Bifidobacterium 40%, Bacillus acidi lactici 25%, saccharomycete 10%.It is wherein described
Bacillus is bacillus subtilis and bacillus amyloliquefaciens in mass ratio 1:1 mixes.
By above-mentioned composite bacteria agent in mass ratio 1:50 and poultry feed（Honest feed）Mixing, for feeding for chicken farm chicken
It supports, complex microbial inoculum of the present invention is added in chicken feed, deodorizing effect is notable, except ammonia rate is up to 79.7%.Chicken manure is dirty
N, P contaminant capacity reduce by 45.8% and 57.9% respectively in dye object.
Embodiment 3
It is a kind of for breeding pollution and the micro-ecological complex bacterial agent as enteron aisle improver simultaneously, each microorganism in composite bacteria agent
Mass fraction in microbial total quality is as shown in table 1.By the compound bacteria in mass ratio 1 of lower column number 1-5:50 and pannage
（Honest feed）Mixing for the nursing of pig, after composite bacteria agent of the present invention, does the ammonia removal rate such as table 1 of cleaning up excrement structure
It is shown.
Table 1：
Claims (7)
1. a kind of be used for breeding pollution and the simultaneously micro-ecological complex bacterial agent as enteron aisle improver, which is characterized in that described multiple
Microbial inoculum is closed by Bacillus foecalis alkaligenes, bacillus, Bifidobacterium, Bacillus acidi lactici, saccharomycete are combined in proportion.
2. according to claim 1 be used for breeding pollution and the simultaneously micro-ecological complex bacterial agent as enteron aisle improver,
Be characterized in that, the bacillus include but not limited to bacillus subtilis, bacillus stearothermophilus, bacillus licheniformis,
Bacillus amyloliquefaciens.
3. according to claim 1 be used for breeding pollution and the simultaneously micro-ecological complex bacterial agent as enteron aisle improver,
It is characterized in that, mass percent of each microorganism in microbial total quality is in the composite bacteria agent：Bacillus foecalis alkaligenes 5 ~
15%th, bacillus 10 ~ 20%, Bifidobacterium 25 ~ 45%, Bacillus acidi lactici 12 ~ 46%, saccharomycete 8 ~ 20%.
4. according to claim 1 be used for breeding pollution and the simultaneously micro-ecological complex bacterial agent as enteron aisle improver,
It is characterized in that, mass percent of each microorganism in microbial total quality is in the composite bacteria agent：Bacillus foecalis alkaligenes 6%,
Bacillus 10%, Bifidobacterium 30%, Bacillus acidi lactici 34%, saccharomycete 20%.
5. according to claim 1 be used for breeding pollution and the simultaneously micro-ecological complex bacterial agent as enteron aisle improver,
It is characterized in that, mass percent of each microorganism in microbial total quality is in the composite bacteria agent：Bacillus foecalis alkaligenes
10%th, bacillus 15%, Bifidobacterium 40%, Bacillus acidi lactici 25%, saccharomycete 10%.
6. according to claim 2 be used for breeding pollution and the simultaneously micro-ecological complex bacterial agent as enteron aisle improver,
It is characterized in that, the bacillus is bacillus subtilis and bacillus licheniformis in mass ratio 1:1 mixes.
7. the Tiny ecosystem for breeding pollution and simultaneously as enteron aisle improver in claim 1-6 described in any one is compound
Microbial inoculum is in the application as feed addictive or breeding pollution renovation agent.
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| CN201711433290.9A CN108085276B (en) | 2017-12-26 | 2017-12-26 | Micro-ecological composite microbial inoculum for breeding pollution and serving as intestinal tract improver at the same time and application thereof |
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| CN201711433290.9A CN108085276B (en) | 2017-12-26 | 2017-12-26 | Micro-ecological composite microbial inoculum for breeding pollution and serving as intestinal tract improver at the same time and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111302501A (en) * | 2020-02-20 | 2020-06-19 | 广东海洋大学深圳研究院 | Method for treating eutrophic seawater by using composite bacteria |
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| JPS63287599A (en) * | 1987-05-20 | 1988-11-24 | Komatsugawa Kakoki Kk | Method for purifying water |
| CN101341928A (en) * | 2008-08-29 | 2009-01-14 | 罗志诣 | Microbial feed additive powder agent for pig and preparation technique |
| CN101341927A (en) * | 2008-08-29 | 2009-01-14 | 罗志诣 | Microbial feed additive liquid agent for pig and preparation technique |
| CN101653200A (en) * | 2009-09-09 | 2010-02-24 | 宝鸡博迪生物科技有限责任公司 | Composite bioactivity feed additive and preparation method thereof |
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-
2017
- 2017-12-26 CN CN201711433290.9A patent/CN108085276B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63287599A (en) * | 1987-05-20 | 1988-11-24 | Komatsugawa Kakoki Kk | Method for purifying water |
| CN101341928A (en) * | 2008-08-29 | 2009-01-14 | 罗志诣 | Microbial feed additive powder agent for pig and preparation technique |
| CN101341927A (en) * | 2008-08-29 | 2009-01-14 | 罗志诣 | Microbial feed additive liquid agent for pig and preparation technique |
| CN101653200A (en) * | 2009-09-09 | 2010-02-24 | 宝鸡博迪生物科技有限责任公司 | Composite bioactivity feed additive and preparation method thereof |
| CN105779335A (en) * | 2016-03-15 | 2016-07-20 | 甘肃省农业科学院生物技术研究所 | Formula and preparation method of micro-ecological deodorant |
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| Title |
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| 付明星: "《淡水养殖动物疾病防治技术》", 30 November 2012, 武汉：湖北科学技术出版社 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111302501A (en) * | 2020-02-20 | 2020-06-19 | 广东海洋大学深圳研究院 | Method for treating eutrophic seawater by using composite bacteria |
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