CN110127858B - Method for treating organic wastewater by using high-salt-resistant strain under low-temperature condition - Google Patents
Method for treating organic wastewater by using high-salt-resistant strain under low-temperature condition Download PDFInfo
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- CN110127858B CN110127858B CN201910398554.4A CN201910398554A CN110127858B CN 110127858 B CN110127858 B CN 110127858B CN 201910398554 A CN201910398554 A CN 201910398554A CN 110127858 B CN110127858 B CN 110127858B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
Abstract
The invention discloses a method for treating organic wastewater by using high-salt-resistant strains under a low-temperature condition, which comprises two parts of culturing low-temperature high-salt-resistant strains and treating organic wastewater by using high-salt-resistant strains, wherein the step of culturing the low-temperature high-salt-resistant strains is to add purified water into an incubator, then add activated sludge of a municipal sewage plant for stuffy aeration for 15-25 h, and control the temperature in the incubator to be 32-35 ℃; cooling to 15-20 ℃, then performing closed aeration circulation for 2-4 days, adding carbohydrase and protease, and continuing the closed aeration circulation for 3-5 days; cooling to 5-10 ℃, continuing to perform aeration for 2-4 days, and adding carbohydrase and protease to obtain low-temperature high-salt-resistant strains and biochemical solution containing the low-temperature high-salt-resistant strains; the microbial film can be quickly established for biochemical reaction, industrial wastewater is treated, and the biochemical period can be shortened to 7-9 days from 9-15 days; and can propagate and survive at 5-15 ℃, and can prolong the relative age. Under the action of several enzymes, it is especially suitable for northern cold area.
Description
Technical Field
The invention belongs to the technical field of wastewater treatment, relates to a method for treating organic wastewater by using sludge, and particularly relates to a method for treating organic wastewater by using high-salt-resistant strains under a low-temperature condition.
Background
In the sewage treatment, the optimal water temperature is 20-35 ℃, the water temperature is below 15 ℃, namely the temperature is low, and compared with inorganic wastewater, organic wastewater is more influenced by the temperature. At present, the treatment methods of organic wastewater comprise an adsorption method, an extraction method, a concentration method, an incineration method, a Fenton oxidation method, an electrochemical oxidation method, an ozone oxidation method, an aerobic biofilm method and the like, but most of the methods have the defects that the cost investment is large, the period is long and the like, so that the application of the methods is limited, especially plants which are easy to generate organic wastewater are provided, most of the plants are low in technological degree and profit, and the popularization is more difficult.
Disclosure of Invention
In order to solve the problems in the prior art, the invention discloses a method for treating organic wastewater by using high-salt-resistant strains under a low-temperature condition, which can quickly establish a microbial film for biochemical reaction and treat industrial wastewater, wherein the biochemical period can be shortened to 7-9 days from 9-15 days; and can propagate and survive at 5-15 ℃, and can prolong the relative age. Under the action of several enzymes, it is especially suitable for northern cold area.
The invention is realized by the following technical scheme:
on one hand, the invention discloses a method for cultivating low-temperature high-salt-resistant strains, which is realized by the following steps:
(1) adding purified water into an incubator, then adding activated sludge of a municipal sewage plant for stuffy aeration for 15-25 h, and controlling the temperature in the incubator at 32-35 ℃;
(2) cooling to 15-20 ℃, then performing closed aeration circulation for 2-4 days, adding carbohydrase and protease, and continuing the closed aeration circulation for 3-5 days;
(3) cooling to 5-10 ℃, continuing to perform aeration for 2-4 days, and adding carbohydrase and protease to obtain low-temperature high-salt-resistant strains and biochemical solution containing the low-temperature high-salt-resistant strains;
and the concentration of carbohydrase in step (3) is lower than the concentration of carbohydrase in step (2), and the concentration of protease in step (3) is lower than the concentration of protease in step (2).
As a preferred embodiment, in the step (1), activated sludge is added into purified water to obtain the sludge concentration of 2500-3500 mg/L, and the aeration is performed for 1.5-2.5 h in the closed aeration process and 7-9 h in the aeration process, and the circulation is performed for 2 times.
In a preferred embodiment, in step (2), the aeration is performed for 3 days and then for 4 days, and the concentration of the carbohydrase and the protease is 5/1000 mg/L.
Further, in the step (3), the aeration is carried out for 3 days, and the concentration of carbohydrase and protease is 1/10000-5/10000 mg/L.
On the other hand, the invention also discloses a method for treating organic wastewater by using high-salt-resistant strains under low temperature, and the biochemical liquid containing the low-temperature high-salt-resistant strains obtained by the cultivation method and industrial wastewater are mixed according to the ratio of 1-2: 1, culturing for 8-10 days in an incubator at 10 +/-2 ℃, then adding low-temperature and high-salt-resistant biochemical liquid, stopping the aeration for 40-55 hours, precipitating and removing sludge; and the adding amount of the biochemical liquid containing low-temperature high-salt resistance is equal to that of the biochemical liquid twice.
Further, mixing biochemical liquid containing low-temperature high-salt-resistant strains and industrial wastewater according to the ratio of 1:1, culturing for 9 days in an incubator at 10 +/-2 ℃, then adding low-temperature and high-salt-resistant biochemical liquid, stopping aeration for 46-50 hours, precipitating and removing sludge.
Compared with the prior art, the invention has the following beneficial effects:
(1) the invention can quickly establish a microbial film for biochemical reaction and treat industrial wastewater, and the biochemical period can be shortened to 7-9 days from 9-15 days;
(2) the low-temperature high-salt-resistant strain can propagate and survive at 5-15 ℃, can prolong the relative age, can perform biochemical reaction under the condition of inferior pollution with salt content of 6-10%, and is particularly suitable for northern cold regions under the action of several enzymes.
Detailed Description
The following description is presented to disclose the invention so as to enable any person skilled in the art to practice the invention. The preferred embodiments in the following description are given by way of example only, and other obvious variations will occur to those skilled in the art.
Example 1
A method for cultivating low-temperature high-salt-resistant strains is realized by the following steps:
(1) adding 2/3 purified water into an incubator, then adding activated sludge of a municipal sewage plant to obtain sludge with the concentration of 3000mg/L, performing aeration for 20 hours, wherein the aeration lasts for 2 hours and 8 hours, circulating for 2 times, and controlling the temperature in the incubator to be 32-35 ℃;
(2) cooling to 15-20 ℃, then performing closed aeration circulation for 3 days, wherein the increase of the fungus death rate can be observed under a microscope, adding carbohydrase and protease to continue the closed aeration circulation for 4 days, and at the moment, observing that only 3-4 single-cell strains exist under an electron microscope, wherein the concentrations of the carbohydrase and the protease are 5/1000mg/L respectively;
(3) and (3) cooling to 5-10 ℃, continuing to perform closed aeration for 3 days, and adding carbohydrase and protease to obtain low-temperature high-salt-resistant strains and biochemical liquid containing the low-temperature high-salt-resistant strains, wherein the concentrations of the carbohydrase and the protease are both 1/10000 mg/L.
A method for utilizing high-salt resistant bacterial to treat organic waste water under the condition of low temperature, mix biochemical fluid and industrial waste water containing low-temperature high-salt resistant bacterial that the above-mentioned cultivation method obtains according to the volume ratio of 1:1, cultivate in 10 duC incubator for 9 days, then add biochemical fluid containing low-temperature high-salt resistant, stop the machine and aerate 48h, precipitate and remove the mud and carry on the chemical analysis to the waste water, the result is shown in table 1; wherein, the adding amount of the biochemical liquid containing low-temperature high-salt resistance is equal to that of the biochemical liquid twice.
| COD（mg/L） | NH3.N mg/L | |
| Original industrial wastewater sample | 7000-9000 | 150-200 |
| Industrial waste Water sample biochemically treated in example 1 | 30-50 | 5-15 |
As can be seen from Table 1, the industrial wastewater treated in this example 1 completely meets the water use standard.
Example 2
A method for cultivating low-temperature high-salt-resistant strains is realized by the following steps:
(1) adding 3/5 purified water into the incubator, then adding activated sludge of a municipal sewage plant to obtain the sludge concentration of 2500mg/L, performing aeration for 17h, wherein the aeration is performed for 1.5h and 7h, the circulation is performed for 2 times, and the temperature in the incubator is controlled to be 32-35 ℃;
(2) cooling to 15-18 ℃, then carrying out closed aeration circulation for 2 days, adding carbohydrase and protease, and continuing the closed aeration circulation for 5 days, wherein the concentrations of the carbohydrase and the protease are both 5/1000 mg/L;
(3) and (3) cooling to 5-8 ℃, continuing to perform closed aeration for 2 days, and adding carbohydrase and protease to obtain low-temperature high-salt-resistant strains and biochemical liquid containing the low-temperature high-salt-resistant strains, wherein the concentrations of the carbohydrase and the protease are both 5/10000 mg/L.
A method for utilizing high-salt resistant bacterial to treat organic waste water under the condition of low temperature, mix biochemical fluid and industrial waste water containing low-temperature high-salt resistant bacterial that the above-mentioned cultivation method obtains according to the volume ratio of 2:1, cultivate in the incubator of 12 duC for 8 days, then add biochemical fluid containing low-temperature high-salt resistant, stop aerating for 40h, precipitate and remove the mud; and the adding amount of the biochemical liquid containing low-temperature high-salt resistance is equal to that of the biochemical liquid twice.
Example 3
A method for cultivating low-temperature high-salt-resistant strains is realized by the following steps:
(1) adding 3/4 purified water into an incubator, then adding activated sludge of a municipal sewage plant to obtain the sludge concentration of 3500mg/L, performing aeration for 23h, wherein the aeration is performed for 2.5h and 9h, the circulation is performed for 2 times, and the temperature in the incubator is controlled to be 32-35 ℃;
(2) cooling to 17-20 ℃, then carrying out closed aeration circulation for 4 days, adding carbohydrase and protease, and continuing the closed aeration circulation for 3 days, wherein the concentrations of the carbohydrase and the protease are both 5/1000 mg/L;
(3) and (3) cooling to 7-10 ℃, continuing to perform closed aeration for 4 days, and adding carbohydrase and protease to obtain low-temperature high-salt-resistant strains and biochemical liquid containing the low-temperature high-salt-resistant strains, wherein the concentrations of the carbohydrase and the protease are both 5/10000 mg/L.
A method for treating organic wastewater by using high-salt-resistant strains at low temperature comprises the steps of mixing biochemical liquid containing low-temperature high-salt-resistant strains obtained by the cultivation method with industrial wastewater according to the volume ratio of 1:1, cultivating the mixture in an incubator at 8 ℃ for 10 days, then adding the biochemical liquid containing low-temperature high-salt-resistant strains, stopping the machine, aerating the mixture for 55 hours, precipitating the mixture and removing sludge; and the adding amount of the biochemical liquid containing low-temperature high-salt resistance is equal to that of the biochemical liquid twice.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (3)
1. The method for cultivating the low-temperature high-salt-resistant strain is characterized by comprising the following steps of:
(1) adding purified water into an incubator, then adding activated sludge of a municipal sewage plant for stuffy aeration for 15-25 h, and controlling the temperature in the incubator at 32-35 ℃;
(2) cooling to 15-20 ℃, then performing closed aeration circulation for 2-4 days, adding carbohydrase and protease, and continuing the closed aeration circulation for 3-5 days;
(3) cooling to 5-10 ℃, continuing to perform aeration for 2-4 days, and adding carbohydrase and protease to obtain low-temperature high-salt-resistant strains and biochemical solution containing the low-temperature high-salt-resistant strains;
the concentration of the carbohydrase in the step (3) is lower than that in the step (2), and the concentration of the protease in the step (3) is lower than that in the step (2);
wherein in the step (1), the activated sludge is added into the purified water to obtain the sludge concentration of 2500-3500 mg/L, and the aeration is performed for 2 times in a closed aeration mode for 1.5-2.5 hours and 7-9 hours; in the step (2), the aeration is firstly carried out for 3 days, then the aeration is carried out for 4 days, and the concentration of carbohydrase and protease is 5/1000 mg/L; in the step (3), the aeration is carried out for 3 days, and the concentration of carbohydrase and protease is 1/10000-5/10000 mg/L.
2. A method for treating organic wastewater by using high-salt-resistant strains at low temperature is characterized by comprising the following steps: mixing the biochemical liquid containing the low-temperature high-salt-resistant strain obtained by the cultivation method of claim 1 with industrial wastewater according to a volume ratio of 2-1: 1, cultivating the mixture in an incubator at 10 +/-2 ℃ for 8-10 days, then adding the biochemical liquid containing the low-temperature high-salt-resistant strain, stopping the incubator, aerating the incubator for 40-55 hours, precipitating and removing sludge; and the adding amount of the biochemical liquid containing low-temperature high-salt resistance is equal to that of the biochemical liquid twice.
3. The method for treating organic wastewater by using high-salt-resistant strains under the low-temperature condition as claimed in claim 2, wherein the biochemical liquid containing the low-temperature high-salt-resistant strains is mixed with the industrial wastewater according to the volume ratio of 1:1, the mixture is cultured in an incubator at 10 +/-2 ℃ for 9 days, then the biochemical liquid containing the low-temperature high-salt-resistant strains is added, the machine is stopped for aeration for 46-50 hours, and precipitation and sludge removal are carried out.
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| US5863434A (en) * | 1994-12-14 | 1999-01-26 | University Of Ottawa/Universite D'ottawa | Psychrophilic anaerobic treatment of waste in a sequencing semibatch/batch bioreactor |
| CN1602677A (en) * | 2004-11-12 | 2005-04-06 | 哈尔滨工业大学 | Low temperature resistant bacteria strain culture method suitable for low temperature daily sewage treatment |
| CN102286390A (en) * | 2010-06-18 | 2011-12-21 | 辽宁北方环境保护有限公司 | Method for screening low temperature resisting nitrifying bacteria |
| CN104164386A (en) * | 2014-07-02 | 2014-11-26 | 中国科学院成都生物研究所 | Method of medium-temperature enrichment culturing and low-temperature application of low-temperature resistant nitrobacterium flora |
| CN108862569A (en) * | 2018-06-15 | 2018-11-23 | 南京理工大学 | The acclimation method of active nitrifying sludge under a kind of low temperature |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5863434A (en) * | 1994-12-14 | 1999-01-26 | University Of Ottawa/Universite D'ottawa | Psychrophilic anaerobic treatment of waste in a sequencing semibatch/batch bioreactor |
| CN1602677A (en) * | 2004-11-12 | 2005-04-06 | 哈尔滨工业大学 | Low temperature resistant bacteria strain culture method suitable for low temperature daily sewage treatment |
| CN102286390A (en) * | 2010-06-18 | 2011-12-21 | 辽宁北方环境保护有限公司 | Method for screening low temperature resisting nitrifying bacteria |
| CN104164386A (en) * | 2014-07-02 | 2014-11-26 | 中国科学院成都生物研究所 | Method of medium-temperature enrichment culturing and low-temperature application of low-temperature resistant nitrobacterium flora |
| CN108862569A (en) * | 2018-06-15 | 2018-11-23 | 南京理工大学 | The acclimation method of active nitrifying sludge under a kind of low temperature |
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