CN114988588B - Method for sewage denitrification by microorganism domestication - Google Patents
Method for sewage denitrification by microorganism domestication Download PDFInfo
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F3/28—Anaerobic digestion processes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N1/36—Adaptation or attenuation of cells
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention is applicable to the technical field of sewage denitrification, and provides a method for sewage denitrification by microbial domestication, which comprises the following steps: s1, preparing a nutrient solution, and adding denitrifying bacteria; s2, initial domestication of low-carbon denitrification strains; s3, secondary domestication of low-carbon denitrification strains; s4, sewage denitrification, the method for sewage denitrification by microbial domestication provided by the invention can activate microorganisms such as anaerobic ammonia oxidation strains and denitrification strains in a biochemical tank, fully utilize carbon sources (internal carbon sources) in the biochemical tank, participate in denitrification reaction through a small amount of external carbon sources added by an electronic device under the condition of insufficient internal carbon sources, save 40-80% of the added external carbon sources, effectively improve the activity of the microorganisms, effectively remove ammonia nitrogen and total nitrogen, improve the biological activity, and simultaneously improve the stability, the effectiveness and the impact resistance of a biochemical system.
Description
Technical Field
The invention relates to the technical field of sewage denitrification, in particular to a method for sewage denitrification by microbial domestication.
Background
Excessive nitrogen and phosphorus nutrients in the sewage enter the water body to cause the pollution of the water body environment and the water quality eutrophication to become serious, so that the abnormal propagation of algae and other microorganisms in the water body to cause the aging of the water body, serious losses are caused to agriculture fishery, aquatic organisms, particularly algae, are propagated in a large quantity, a large number of dead aquatic organisms are decomposed by the microorganisms, a large amount of dissolved oxygen is consumed in the decomposition process, and the concentration of the dissolved oxygen in the water is rapidly reduced, so that the survival of aquatic organisms such as fishes is influenced.
Most of the existing sewage denitrification is treated by chemical means, and chemical agents are added into the water body, so that other chemical substances can be introduced into the water body by the treatment mode, the ecological balance of the water body is affected, the treatment cost is high, and the treatment effect is poor.
Disclosure of Invention
The invention aims to provide a method for sewage denitrification by microbial domestication, which aims to solve the problems that in the prior art, most of sewage denitrification is treated by chemical means, other chemical substances are introduced into a water body to influence the ecological balance of the water body, the treatment cost is high and the treatment effect is poor.
In order to achieve the above purpose, the present invention provides the following technical solutions: a method for sewage denitrification by microorganism domestication, comprising the following steps:
s1, preparing a nutrient solution, and adding denitrifying bacteria:
preparing a nutrient solution with the concentration of C, N and P of 100:5:1, and placing the nutrient solution in a range of 1-5 m 3 Adding denitrifying bacteria 1-10%, sealing and stirring, and the pH value is 7.0-8.5;
s2, initial domestication of low-carbon denitrification strains:
s21, sucking air through an exhaust fan, performing magnetic field treatment, and slowly introducing the air subjected to the magnetic field treatment into the container;
s22, when the total nitrogen removal rate in the nutrient solution reaches more than 95%, adding additional nutrient solution into the container, enabling the ratio of C to N to P in the container to be 80:5:1, when the total nitrogen removal rate in the nutrient solution reaches more than 95%, adding additional nutrient solution into the container again, and repeating the operation until the ratio of C to N to P in the container is 60:5:1, 50:5:1 and 40:5:1 in sequence, wherein the ratio of C to N to P in the container is 30:5:1;
s23, the whole domestication process is carried out for 7-10 days, so as to obtain low-carbon denitrification strains;
s3, secondary domestication of low-carbon denitrification strains:
adding low-carbon denitrification strains into a denitrification tank, an aeration aerobic tank and a sedimentation tank in a biochemical system, and further domesticating the low-carbon denitrification strains by wastewater to be treated and activated sludge to be domesticated in the denitrification tank, the aeration aerobic tank and the sedimentation tank, wherein the low-carbon denitrification strains are specifically as follows:
s31, adding the low-carbon denitrification strains into a denitrification tank of a biochemical system according to the addition amount of 10-20% of the tank capacity of the denitrification tank every 24-48 h, and slowly introducing air subjected to magnetic field treatment into the denitrification tank to enable the low-carbon denitrification strains to gradually adapt to the living environment of the biochemical system;
s32, the residence time of the low-carbon denitrification strain in the denitrification tank is 6-8 h, the residence time of the aeration aerobic tank is 24-48 h, the residence time of the sedimentation tank is 3-4 h, and if the circulation is repeated, the domestication time is 10-20 d;
s4, sewage denitrification:
after the domestication is finished, the wastewater to be treated enters water according to the treatment capacity of 10-20% per day until the wastewater runs at full load, and a small amount of external carbon source is added through an electronic device to participate in denitrification reaction under the condition of insufficient internal carbon source.
Preferably, the stirring speed in the step S1 is 50-100 rpm/min.
Preferably, the magnetic field strength in S2 is 10. Mu.T to 1000. Mu.T.
Preferably, in the step S2, air subjected to magnetic field treatment is introduced into the container, and dissolved oxygen in the nutrient solution is ensured to be less than or equal to 0.5mg/L.
Preferably, the pH value in the domestication process in the S23 is kept between 7.0 and 8.5, and the temperature is between 25 and 32 ℃.
The invention has at least the following beneficial effects:
(1) The method for sewage denitrification by microbial domestication provided by the invention can activate microorganisms such as anaerobic ammonia oxidation strains and denitrification strains in a biochemical tank, fully utilize carbon sources (internal carbon sources) in the biochemical tank, participate in denitrification reaction by a small amount of external carbon sources added by an electronic device under the condition of insufficient internal carbon sources, and save 40-80% of the added external carbon sources;
(2) The method for sewage denitrification by microbial domestication provided by the invention effectively improves the activity of microorganisms, effectively removes ammonia nitrogen and total nitrogen, improves the biological activity, and simultaneously improves the stability, the effectiveness and the impact resistance of a biochemical system;
(3) According to the method for sewage denitrification by microbial domestication, provided by the invention, the addition amount of an external carbon source is reduced, so that the generated sludge is also greatly reduced, and the cost of sludge disposal is saved.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Examples
A method for sewage denitrification by microorganism domestication, comprising the following steps:
s1, preparing a nutrient solution, and adding denitrifying bacteria:
preparing a nutrient solution with the concentration of C, N and P of 100:5:1, and placing the nutrient solution in a range of 1-5 m 3 Adding denitrifying bacteria 1-10%, sealing and stirring at 50-100 rpm/min and pH value of 7.0-8.5;
s2, initial domestication of low-carbon denitrification strains:
s21, sucking air through an exhaust fan, treating the air by a magnetic field with the magnetic field strength of 10 mu T-1000 mu T, slowly introducing the air subjected to the magnetic field treatment into a container, ensuring that dissolved oxygen in the nutrient solution is less than or equal to 0.5mg/L, generating certain electromagnetic waves and charged air particles by the air subjected to the electromagnetic field treatment, and enabling the electromagnetic waves to stimulate the growth and propagation of microorganisms in the nutrient solution to decompose and digest the nutrient solution in the container;
s22, when the total nitrogen removal rate in the nutrient solution reaches more than 95%, adding additional nutrient solution into a container, enabling the total nitrogen removal rate in the container to be more than 95% as C: N: P in the container is 80:5:1, adding additional nutrient solution into the container again, repeating the operation, and enabling the total nitrogen removal rate in the container to be obviously reduced until the total nitrogen removal rate in the nutrient solution C: N: P is 30:5:1, wherein the total nitrogen removal rate is 60:5:1, 50:5:1 and 40:5:1 in sequence until the total nitrogen removal rate in the nutrient solution C: N: P is 30:5:1, wherein charged air particles can replace a carbon source as an electron donor to stimulate the propagation metabolism of microorganisms in cooperation with electromagnetic waves due to the reduction of the carbon source, but the microorganisms can preferentially select the carbon source as the electron donor to consume organic matters under the condition that the carbon source is sufficient;
s23, maintaining the pH value of the domestication process at 7.0-8.5 and the temperature at 25-32 ℃, wherein the domesticated denitrifying bacteria are suitable for denitrification reaction with low carbon concentration and denitrification process by using charged air particles as electron donors, and the whole domestication process is 7-10 d to obtain the low carbon denitrifying bacteria;
s3, secondary domestication of low-carbon denitrification strains:
adding low-carbon denitrification strains into a denitrification tank, an aeration aerobic tank and a sedimentation tank in a biochemical system, and further domesticating the low-carbon denitrification strains by wastewater to be treated and activated sludge to be domesticated in the denitrification tank, the aeration aerobic tank and the sedimentation tank, wherein the low-carbon denitrification strains are specifically as follows:
s31, adding the low-carbon denitrification strains into a denitrification tank of a biochemical system according to the addition amount of 10-20% of the tank capacity of the denitrification tank every 24-48 h, and slowly introducing air subjected to magnetic field treatment into the denitrification tank to enable the low-carbon denitrification strains to gradually adapt to the living environment of the biochemical system;
s32, the residence time of the low-carbon denitrification strain in the denitrification tank is 6-8 h, the residence time of the aeration aerobic tank is 24-48 h, the residence time of the sedimentation tank is 3-4 h, and if the circulation is repeated, the domestication time is 10-20 d;
s4, sewage denitrification:
after the domestication is finished, the wastewater to be treated enters water according to the treatment capacity of 10-20% per day until the wastewater runs at full load, and a small amount of external carbon source is added through an electronic device to participate in denitrification reaction under the condition of insufficient internal carbon source.
The method for sewage denitrification by microbial domestication provided by the invention fully utilizes the carbon source in the biochemical tank, improves the bioactivity, and simultaneously improves the stability, the effectiveness and the impact resistance of the biochemical system, thereby greatly reducing the generated sludge and saving the cost of sludge disposal.
While the fundamental and principal features of the invention and advantages of the invention have been shown and described, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing exemplary embodiments, but may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (4)
1. A method for sewage denitrification by microbial domestication, which is characterized by comprising the following steps:
s1, preparing a nutrient solution, and adding denitrifying bacteria:
preparing a nutrient solution with the concentration of C, N and P of 100:5:1, and placing the nutrient solution in a range of 1-5 m 3 Adding denitrifying bacteria 1-10%, sealing and stirring, and the pH value is 7.0-8.5;
s2, initial domestication of low-carbon denitrification strains:
s21, sucking air through an exhaust fan, performing magnetic field treatment, and slowly introducing the air subjected to the magnetic field treatment into the container;
s22, when the total nitrogen removal rate in the nutrient solution reaches more than 95%, adding additional nutrient solution into the container, enabling the ratio of C to N to P in the container to be 80:5:1, when the total nitrogen removal rate in the nutrient solution reaches more than 95%, adding additional nutrient solution into the container again, and repeating the operation until the ratio of C to N to P in the container is 60:5:1, 50:5:1 and 40:5:1 in sequence, wherein the ratio of C to N to P in the container is 30:5:1;
s23, the whole domestication process is carried out for 7-10 days, the pH value in the domestication process is kept at 7.0-8.5, and the temperature is 25-32 ℃, so that the low-carbon denitrification strain is obtained;
s3, secondary domestication of low-carbon denitrification strains:
adding low-carbon denitrification strains into a denitrification tank, an aeration aerobic tank and a sedimentation tank in a biochemical system, and further domesticating the low-carbon denitrification strains by wastewater to be treated and activated sludge to be domesticated in the denitrification tank, the aeration aerobic tank and the sedimentation tank, wherein the low-carbon denitrification strains are specifically as follows:
s31, adding the low-carbon denitrification strains into a denitrification tank of a biochemical system according to the addition amount of 10-20% of the tank capacity of the denitrification tank every 24-48 h, and slowly introducing air subjected to magnetic field treatment into the denitrification tank to enable the low-carbon denitrification strains to gradually adapt to the living environment of the biochemical system;
s32, the residence time of the low-carbon denitrification strain in the denitrification tank is 6-8 h, the residence time of the aeration aerobic tank is 24-48 h, the residence time of the sedimentation tank is 3-4 h, and if the circulation is repeated, the domestication time is 10-20 d;
s4, sewage denitrification:
after the domestication is finished, the wastewater to be treated enters water according to the treatment capacity of 10-20% per day until the wastewater runs at full load, and a small amount of external carbon source is added through an electronic device to participate in denitrification reaction under the condition of insufficient internal carbon source.
2. The method for sewage denitrification by microbial domestication according to claim 1, wherein: the stirring speed in the step S1 is 50-100 rpm/min.
3. The method for sewage denitrification by microbial domestication according to claim 1, wherein: the magnetic field intensity in the S2 is 10 mu T-1000 mu T.
4. The method for sewage denitrification by microbial domestication according to claim 1, wherein: and (2) introducing air subjected to magnetic field treatment into the container in the step (S2), and ensuring that the dissolved oxygen in the nutrient solution is less than or equal to 0.5mg/L.
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