CN111500481A - Domestication culture method of sulfur autotrophic denitrifying bacteria and application of domestication culture method - Google Patents
Domestication culture method of sulfur autotrophic denitrifying bacteria and application of domestication culture method Download PDFInfo
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- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 title claims abstract description 57
- 230000001651 autotrophic effect Effects 0.000 title claims abstract description 53
- 229910052717 sulfur Inorganic materials 0.000 title claims abstract description 51
- 239000011593 sulfur Substances 0.000 title claims abstract description 51
- 241000894006 Bacteria Species 0.000 title claims abstract description 47
- 238000012136 culture method Methods 0.000 title claims abstract description 29
- 239000010802 sludge Substances 0.000 claims abstract description 26
- 229910002651 NO3 Inorganic materials 0.000 claims abstract description 13
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 11
- 239000011259 mixed solution Substances 0.000 claims abstract description 11
- 239000000243 solution Substances 0.000 claims abstract description 10
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000012258 culturing Methods 0.000 claims abstract description 7
- 239000000758 substrate Substances 0.000 claims abstract description 5
- 230000000737 periodic effect Effects 0.000 claims abstract description 4
- 239000001963 growth medium Substances 0.000 claims description 14
- 229910052799 carbon Inorganic materials 0.000 claims description 9
- 239000010865 sewage Substances 0.000 claims description 7
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 239000007789 gas Substances 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 claims description 4
- 229910020350 Na2WO4 Inorganic materials 0.000 claims description 3
- 239000005864 Sulphur Substances 0.000 claims description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 3
- 229910052782 aluminium Inorganic materials 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 229920005549 butyl rubber Polymers 0.000 claims description 3
- 239000000920 calcium hydroxide Substances 0.000 claims description 3
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims description 3
- 239000000306 component Substances 0.000 claims description 3
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 3
- 229910000366 copper(II) sulfate Inorganic materials 0.000 claims description 3
- 238000006477 desulfuration reaction Methods 0.000 claims description 3
- 230000023556 desulfurization Effects 0.000 claims description 3
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 3
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000008188 pellet Substances 0.000 claims description 3
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 3
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 3
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Inorganic materials [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 3
- XMVONEAAOPAGAO-UHFFFAOYSA-N sodium tungstate Chemical compound [Na+].[Na+].[O-][W]([O-])(=O)=O XMVONEAAOPAGAO-UHFFFAOYSA-N 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- DHCDFWKWKRSZHF-UHFFFAOYSA-N sulfurothioic S-acid Chemical compound OS(O)(=O)=S DHCDFWKWKRSZHF-UHFFFAOYSA-N 0.000 claims description 3
- 239000002351 wastewater Substances 0.000 claims description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
- 239000011686 zinc sulphate Substances 0.000 claims description 3
- 239000008187 granular material Substances 0.000 claims 2
- 229910019626 (NH4)6Mo7O24 Inorganic materials 0.000 claims 1
- 235000019341 magnesium sulphate Nutrition 0.000 claims 1
- 235000009529 zinc sulphate Nutrition 0.000 claims 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000002245 particle Substances 0.000 description 4
- MWUXSHHQAYIFBG-UHFFFAOYSA-N nitrogen oxide Inorganic materials O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 229910018143 SeO3 Inorganic materials 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 150000004763 sulfides Chemical class 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
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Abstract
The invention discloses a domestication culture method of sulfur autotrophic denitrifying bacteria and application thereof, wherein the bacteria adopt a culture mode of periodic operation, and the method comprises the following steps of adding an electron donor and inoculated sludge into a culture substrate with a pre-prepared nitrate concentration of 40 mg/L to obtain a mixed solution, culturing and domesticating the sulfur autotrophic denitrifying bacteria under the culture condition of pH7.0-7.3, a temperature of 30 +/-5 ℃, a rotating speed of 150rpm, measuring the nitrate concentration and ammonia nitrogen removal efficiency of the mixed solution every 5d, recording the time after the ammonia nitrogen removal rate is stabilized to 90%, ending a domestication period, increasing the concentration of the nitrate solution in the culture substrate, and carrying out domestication in the next period according to the gradient of 40, 60, 80, 100 and 120 mg/L until the domestication is completed.
Description
Technical Field
The invention belongs to the technical field of eutrophic water body treatment, and particularly relates to a domestication culture method of sulfur autotrophic denitrifying bacteria and application thereof.
Background
The biofilm method is widely used for treating micro-polluted eutrophic water as a low-cost and high-efficiency sewage treatment method. The widely accepted metabolic pathways for nitrogen removal in biofilm reactors mainly include two types of nitrification and denitrification. Traditionally, nitrification has been accomplished primarily by autotrophic bacteria in an aerobic environment, while denitrification has been accomplished primarily by heterotrophic bacteria in an anoxic or anaerobic environment by electron donors. Previous studies have shown that efficient nitrification is easy to achieve but also very likely to result in the accumulation of nitrate and nitrite nitrogen in a biofilm reactor. In addition, studies have now shown that denitrification is divided into two types, heterotrophic and autotrophic, with heterotrophic denitrification relying primarily on available carbon sources as electron donors and autotrophic denitrification relying primarily on iron, hydrogen or sulfides as electron donors. In the treatment process of the micro-polluted eutrophic water body, denitrification is often the limiting factor for realizing efficient synchronous nitrification and denitrification, and the denitrification effect is not ideal due to the low C/N, insufficient carbon source and the like. Therefore, in recent years, efficient denitrification through external carbon sources has become a hot point of research, but the addition of external reinforcement carbon sources has the defect that the effective carbon sources released by the materials are often not stable enough. Therefore, the research on the transfer of a large part of the gravity center to the optimization of the performance of the synchronous nitrification and denitrification through the screening of the solid carbon source. In recent years, the combination of autotrophic denitrification and the realization of an efficient denitrification function becomes a great research hotspot, compared with single heterotrophic denitrification, the autotrophic denitrification can get rid of the limitation of available organic matters, reduce the generation amount of sludge and the release of nitrogen oxides, and greatly improve the denitrification performance, such as adding sulfur or sulfide into a bioreactor of autotrophic denitrification, although the denitrification performance is greatly improved. One of the most important limiting factors is the activity of the sulfur autotrophic denitrifying bacteria, which greatly influences the TN removal rate of the sulfur autotrophic denitrifying bacteria in denitrification.
Disclosure of Invention
The invention aims to provide a domestication culture method of sulfur autotrophic denitrifying bacteria and application thereof, which can effectively carry out domestication culture and enrichment on the sulfur autotrophic denitrifying bacteria and improve the performance of the sulfur autotrophic denitrifying bacteria in denitrification.
The invention aims to provide a domestication culture method of sulfur autotrophic denitrifying bacteria, which adopts a culture mode of periodic operation and comprises the following steps:
adding an electron donor and inoculated sludge into a culture medium with a pre-prepared nitrate concentration of 40 mg/L to obtain a mixed solution, culturing and domesticating sulfur autotrophic denitrifying bacteria under the culture condition of pH7.0-7.3, at the temperature of 30 +/-5 ℃, at the rotating speed of 150rpm, measuring the nitrate concentration and ammonia nitrogen removal efficiency of the mixed solution every 5d, recording the time after the ammonia nitrogen removal rate is stabilized to 90%, and finishing a domestication period;
and step two, after the mixed solution is precipitated, pouring out the supernatant, increasing the concentration of the nitrate solution in the culture substrate, and performing domestication for the next period according to the gradient of 40, 60, 80, 100 and 120 mg/L until the domestication is finished.
Further, the domestication culture method of the sulfur autotrophic denitrifying bacteria comprises the following culture medium components: sulphur pellets 40g, NaNO340-120g/L， NH4HCO30.41g/L，K2HPO40.25g/L，Ca(OH)20.005g/L，NaHCO32.70g/L，MgSO4•7H2O0.10 g/L, and 2m L microelement solution, wherein the microelement solution is FeSO 2.800 g/L4•7H2CoSO of O, 2.360 g/L4•7H2O，ZnSO4•7H2O0.106 g/L, Na 0.1 g/L2SeO3•5H2O, 0.415 g/L MnSO4•H2O, 0.157 g/L CuSO4•5H2O, 0.050 g/L (NH)4)6Mo7O24•H2O, 0.500 g/L Na2WO4•2H2O or a combination of several O.
Further, according to the acclimatization and culture method of the sulfur autotrophic denitrifying bacteria, the inoculated sludge is prepared according to the following method, a proper amount of anaerobic sludge of a sewage plant is taken, the mixture is kept stand for 4 hours, then supernatant is discarded, 300m L of concentrated sludge of the sewage plant is placed in a 1L anaerobic culture bottle, a culture medium of 400m L is added into the anaerobic culture bottle, and then sulfur particles with the particle size of 1-3mm are added.
Further, the domestication and culture method of the sulfur autotrophic denitrifying bacteria further comprises a pretreatment step of sterilizing the culture medium added into the anaerobic culture bottle, deoxidizing the upper space of the anaerobic culture bottle, and placing the anaerobic culture bottle after the pretreatment step into a constant temperature shaking table for dark culture at the temperature of 30 +/-5 ℃ and the rotating speed of 150rpm before the culture is started.
Further, in the acclimatization and culture method of the sulfur autotrophic denitrifying bacteria, the deoxidation treatment is to introduce N into an anaerobic culture bottle2And CO2The mixed gas is discharged for 2-4min, the bottle mouth is immediately plugged by a butyl rubber plug after oxygen in the upper space of the anaerobic culture bottle is discharged, and then the bottle is further sealed by an aluminum seal.
Further, the acclimatization and culture method of the sulfur autotrophic denitrifying bacteria of the present invention, N2And CO2N in the mixed gas of2And CO2Is 4: 1.
Further, in the acclimatization and culture method of the sulfur autotrophic denitrifying bacteria, under an anaerobic condition, the electron donor is one or a combination of more of thiosulfate, elemental sulfur or sulfide.
The invention also aims to apply the sulfur autotrophic denitrifying bacteria to the sulfur autotrophic denitrification denitrogenation and desulfurization of the wastewater, wherein the sulfur autotrophic denitrifying bacteria are prepared according to the method.
Has the advantages that: the enrichment acclimation shows that the characters of the inoculated sludge are obviously changed from the original dark black and scattered sludge to light grey and flocculent sludge which is in an aerobic sludge state compared with the characters before the acclimation, so that the denitrification effect of the activated sludge in the aerobic denitrification is stable, and the TN removal rate is ensured to be more than 50 percent.
Detailed Description
The method for acclimatizing and culturing sulfur autotrophic denitrifying bacteria according to the present invention will be described in detail with reference to the following embodiments.
The domestication culture method of the sulfur autotrophic denitrifying bacteria adopts a culture mode of periodic operation, and comprises the following steps:
adding an electron donor and inoculated sludge into a culture medium with a pre-prepared nitrate concentration of 40 mg/L to obtain a mixed solution, culturing and domesticating sulfur autotrophic denitrifying bacteria under the culture condition of pH7.0-7.3, at the temperature of 30 +/-5 ℃, at the rotating speed of 150rpm, measuring the nitrate concentration and ammonia nitrogen removal efficiency of the mixed solution every 5d, recording the time after the ammonia nitrogen removal rate is stabilized to 90%, and finishing a domestication period;
and step two, after the mixed solution is precipitated, pouring out the supernatant, increasing the concentration of the nitrate solution in the culture substrate, and performing domestication for the next period according to the gradient of 40, 60, 80, 100 and 120 mg/L until the domestication is finished.
Further, the domestication culture method of the sulfur autotrophic denitrifying bacteria comprises the following culture medium components: sulphur pellets 40g, NaNO340-120g/L，NH4HCO30.41g/L，K2HPO40.25g/L，Ca(OH)20.005g/L，NaHCO32.70g/L，MgSO4•7H2O0.10 g/L, and 2m L microelement solution, wherein the microelement solution is FeSO 2.800 g/L4•7H2CoSO of O, 2.360 g/L4•7H2O，ZnSO4•7H2O0.106 g/L, Na 0.1 g/L2SeO3•5H2O, 0.415 g/L MnSO4•H2O, 0.157 g/L CuSO4•5H2O, 0.050 g/L (NH)4)6Mo7O24•H2O, 0.500 g/L Na2WO4•2H2O or a combination of several O.
Further, according to the acclimatization and culture method of the sulfur autotrophic denitrifying bacteria, the inoculated sludge is prepared according to the following method, a proper amount of anaerobic sludge of a sewage plant is taken, the mixture is kept stand for 4 hours, then supernatant is discarded, 300m L of concentrated sludge of the sewage plant is placed in a 1L anaerobic culture bottle, a culture medium of 400m L is added into the anaerobic culture bottle, and then sulfur particles with the particle size of 1-3mm are added.
Further, the domestication and culture method of the sulfur autotrophic denitrifying bacteria further comprises a pretreatment step of sterilizing the culture medium added into the anaerobic culture bottle, deoxidizing the upper space of the anaerobic culture bottle, and placing the anaerobic culture bottle after the pretreatment step into a constant temperature shaking table for dark culture at the temperature of 30 +/-5 ℃ and the rotating speed of 150rpm before the culture is started.
Further, in the acclimatization and culture method of the sulfur autotrophic denitrifying bacteria, the deoxidation treatment is to introduce N into an anaerobic culture bottle2And CO2The mixed gas is discharged for 2-4min, the bottle mouth is immediately plugged by a butyl rubber plug after oxygen in the upper space of the anaerobic culture bottle is discharged, and then the bottle is further sealed by an aluminum seal.
Further, the acclimatization and culture method of the sulfur autotrophic denitrifying bacteria of the present invention, N2And CO2N in the mixed gas of2And CO2Is 4: 1.
Further, in the acclimatization and culture method of the sulfur autotrophic denitrifying bacteria, under an anaerobic condition, the electron donor is one or a combination of more of thiosulfate, elemental sulfur or sulfide.
The invention also aims to apply the sulfur autotrophic denitrifying bacteria to the sulfur autotrophic denitrification denitrogenation and desulfurization of the wastewater, wherein the sulfur autotrophic denitrifying bacteria are prepared according to the method.
The domestication and culture method of the sulfur autotrophic denitrifying bacteria is simple and convenient to operate, the domesticated sulfur autotrophic denitrifying bacteria have high activity, and the character of the inoculated sludge is obviously changed from the original dark black and scattered sludge to light grey and flocculent sludge and is in an aerobic sludge state through enrichment and domestication compared with the sludge before domestication, so that the denitrification effect of the activated sludge in the aerobic denitrification is stable, and the ammonia nitrogen removal rate is ensured to be over 90 percent.
Claims (8)
1. A domestication culture method of sulfur autotrophic denitrifying bacteria is characterized in that a culture mode of periodic operation is adopted, and the domestication culture method comprises the following steps:
adding an electron donor and inoculated sludge into a culture medium with a pre-prepared nitrate concentration of 40 mg/L to obtain a mixed solution, culturing and domesticating sulfur autotrophic denitrifying bacteria under the culture condition of pH7.0-7.3, at the temperature of 30 +/-5 ℃, at the rotating speed of 150rpm, measuring the nitrate concentration and ammonia nitrogen removal efficiency of the mixed solution every 5d, recording the time after the ammonia nitrogen removal rate is stabilized to 90%, and finishing a domestication period;
and step two, after the mixed solution is precipitated, pouring out the supernatant, increasing the concentration of the nitrate solution in the culture substrate, and performing domestication for the next period according to the gradient of 40, 60, 80, 100 and 120 mg/L until the domestication is finished.
2. The acclimatization and culture method of sulfur autotrophic denitrifying bacteria according to claim 1, wherein the culture medium components comprise: sulphur pellets 40g, NaNO340-120g/L， NH4HCO30.41g/L，K2HPO40.25g/L，Ca(OH)20.005g/L，NaHCO32.70g/L，MgSO4•7H2O0.10 g/L, and 2m L microelement solution, wherein the microelement solution is FeSO 2.800 g/L4•7H2CoSO of O, 2.360 g/L4•7H2O，ZnSO4•7H2O0.106 g/L, Na2SeO 0.1 g/L3•5H2O, 0.415 g/L MnSO4. H2O, 0.157 g/L CuSO4•5H2O, 0.050 g/L of (NH4)6Mo7O24•H2O, 0.500 g/L Na2WO4•2H2O or a combination of several O.
3. The acclimatization and culture method of sulfur autotrophic denitrifying bacteria according to claim 1, wherein the inoculated sludge is prepared by taking a proper amount of anaerobic sludge from sewage plants, standing for 4h, then discarding the supernatant, taking 300m L concentrated sludge from sewage plants, placing the sludge in a 1L anaerobic culture bottle, adding culture medium 400m L into the anaerobic culture bottle, and then adding sulfur granules with the granule diameter of 1-3 mm.
4. The acclimatization and culture method of sulfur autotrophic denitrifying bacteria according to claim 1, further comprising a pretreatment step of sterilizing the culture medium added to the anaerobic culture bottle and deoxidizing the upper space of the anaerobic culture bottle before the start of culture, wherein the anaerobic culture bottle after the pretreatment step is placed in a constant temperature shaking table for dark culture at a temperature of 30 ± 5 ℃ and a rotation speed of 150 rpm.
5. The method for acclimatizing and culturing sulfur autotrophic denitrifying bacteria according to claim 1, wherein said culture medium,
the deoxidation treatment is to introduce N into an anaerobic culture bottle2And CO2The mixed gas is discharged for 2-4min, the bottle mouth is immediately plugged by a butyl rubber plug after oxygen in the upper space of the anaerobic culture bottle is discharged, and then the bottle is further sealed by an aluminum seal.
6. The method for acclimatizing and culturing sulfur autotrophic denitrifying bacteria according to claim 1, wherein said culture medium,
N2and CO2N in the mixed gas of2And CO2Is 4: 1.
7. The acclimatization and culture method of sulfur autotrophic denitrifying bacteria according to claim 1, wherein the electron donor is one or a combination of thiosulfate, elemental sulfur or sulfide under anaerobic conditions.
8. The sulfur autotrophic denitrifying bacteria are applied to the sulfur autotrophic denitrification denitrogenation and desulfurization of wastewater, and are characterized in that the sulfur autotrophic denitrifying bacteria are prepared according to the domestication culture method of the sulfur autotrophic denitrifying bacteria of any one of claims 1 to 8.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113087142A (en) * | 2021-04-16 | 2021-07-09 | 北京涞澈科技发展有限公司 | Rapid start accelerator for sulfur autotrophic microorganisms in high-nitrogen domestic sewage |
| CN114437981A (en) * | 2022-02-15 | 2022-05-06 | 科盛环保科技股份有限公司 | Fermentation method for rapidly culturing sulfur autotrophic denitrifying bacteria |
| CN114988588A (en) * | 2022-05-31 | 2022-09-02 | 江西调水人生态环境工程有限公司 | Method for sewage denitrification through microorganism domestication |
| CN115536144A (en) * | 2021-06-29 | 2022-12-30 | 中国石油化工股份有限公司 | Rapid enrichment method and device for sulfur autotrophic denitrifying bacteria |
| CN117303558A (en) * | 2023-11-15 | 2023-12-29 | 广东顺控自华科技有限公司 | Method for denitrification by sulfur autotrophy-heterotrophy synergy under low sulfur load condition |
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| CN101434905A (en) * | 2007-11-15 | 2009-05-20 | 中国石油化工股份有限公司 | Method for screening nitrification bacteria |
| US20110084022A1 (en) * | 2009-10-09 | 2011-04-14 | Leaderman & Associates Co., Ltd. | Process for treating nitrogenous wastewater with simultaneous autotrophic denitrification, hetertrophic denitrification and COD removal |
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| CN115536144B (en) * | 2021-06-29 | 2024-05-03 | 中国石油化工股份有限公司 | Method and device for rapidly enriching sulfur autotrophic denitrifying bacteria |
| CN114437981A (en) * | 2022-02-15 | 2022-05-06 | 科盛环保科技股份有限公司 | Fermentation method for rapidly culturing sulfur autotrophic denitrifying bacteria |
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| CN114988588B (en) * | 2022-05-31 | 2023-09-19 | 江西调水人生态环境工程有限公司 | Method for sewage denitrification by microorganism domestication |
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