CN111606509A - Low-temperature bacterium proportioning culture method for sewage treatment - Google Patents
Low-temperature bacterium proportioning culture method for sewage treatment Download PDFInfo
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- 239000010865 sewage Substances 0.000 title claims abstract description 59
- 241000894006 Bacteria Species 0.000 title claims abstract description 36
- 238000012136 culture method Methods 0.000 title claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 23
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 13
- 239000011550 stock solution Substances 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 17
- 229910052760 oxygen Inorganic materials 0.000 claims description 17
- 239000001301 oxygen Substances 0.000 claims description 17
- 238000004062 sedimentation Methods 0.000 claims description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
- 230000001105 regulatory effect Effects 0.000 claims description 8
- 238000005273 aeration Methods 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 4
- 230000003213 activating effect Effects 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 230000001546 nitrifying effect Effects 0.000 claims description 3
- 230000001502 supplementing effect Effects 0.000 claims description 3
- 241001148470 aerobic bacillus Species 0.000 claims 2
- 239000000243 solution Substances 0.000 claims 1
- 239000010802 sludge Substances 0.000 abstract description 10
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- 230000015556 catabolic process Effects 0.000 abstract description 4
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- 238000010438 heat treatment Methods 0.000 abstract description 4
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- 241000233866 Fungi Species 0.000 description 2
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- 230000009286 beneficial effect Effects 0.000 description 2
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- 235000009508 confectionery Nutrition 0.000 description 2
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- 235000015097 nutrients Nutrition 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000131970 Rhodospirillaceae Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
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- 238000010276 construction Methods 0.000 description 1
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- 241000894007 species Species 0.000 description 1
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F7/00—Aeration of stretches of water
Abstract
The invention discloses a low-temperature bacterium proportioning culture method for sewage treatment, and belongs to the technical field of sewage treatment. It comprises the following steps: (1) building sewage treatment equipment; (2) adding strains for one time; (3) adding strains for the second time; (4) adding the stock solution of photosynthetic bacteria. The reasonable strain proportion and the proper activation and domestication method generate high-efficiency and low-temperature-resistant low-temperature strains; the low-temperature strain has higher treatment efficiency for treating domestic sewage in a low-temperature environment, and compared with a common heat preservation or heating method, the energy consumption cost is much lower; the low-temperature bacterium proportioning culture method can solve the problem of sludge bulking caused by low temperature; the combination of different strains can more effectively strengthen the denitrification and COD degradation performance of the system.
Description
Technical Field
The invention relates to a low-temperature bacterium proportioning culture method for sewage treatment, and belongs to the technical field of sewage treatment.
Background
In sewage treatment, the microorganism is mainly different from other chemical agents and is characterized by activity, so that the microorganism is greatly influenced by temperature in the aspects of growth, reproduction, metabolism and the like, and the temperature is one of the most important environmental factors influencing the growth of the microorganism. The requirements of different microorganisms on temperature are different, and in an improper temperature environment, the activity of the microorganisms is reduced, the sewage treatment effect is poor, and even the condition that the microorganisms can not grow normally and the quality of effluent is seriously influenced occurs.
The method has the advantages that the width of our country is wide, four seasons are clear, northern areas are cold and long in winter, and the activity of various microorganisms in the sewage treatment process in the areas is reduced under severe weather conditions, so that the sludge expansion phenomenon can occur, and the sewage treatment effect is influenced. At present, in engineering practice in cold regions in China, the treatment effect of sewage is generally improved by prolonging the retention time of the sewage, reducing the sludge load, increasing the sludge backflow and the like, and the heat preservation or heating mode of a process unit is also common, but the measures can greatly increase the engineering cost and also increase the technical difficulty of sewage treatment plants and the like for temperature control.
A limiting factor in the growth of microorganisms in low temperature environments is whether the cells are able to transport external nutrients into the cells at low temperatures. In general, mesophilic microorganisms cannot metabolize exogenous substances at a temperature lower than 5 ℃, and only cryobacteria can normally transport external glucose and other nutrients into the body at a temperature of 2 ℃. Under a low-temperature environment, the biological activity of other microorganisms which cannot be replaced by the low-temperature bacteria can improve the sewage treatment efficiency, and the low-temperature bacteria have wide application prospects; the research on the proportioning mode of the low-temperature bacteria not only can make a great breakthrough in the research aspect of low-temperature microorganisms, but also has great help on production practice and environmental protection, and can further reduce the construction and operation costs of sewage treatment projects in cold regions. Therefore, from the biological perspective, on the basis of mastering the influence of low temperature on the performance of activated sludge, a low-temperature bacterium capable of normally and efficiently treating sewage in a low-temperature environment is researched, and the method are fundamental measures and methods for really and effectively solving the adverse influence of temperature on the sewage treatment effect.
Therefore, the low-temperature bacteria proportioning culture method for sewage treatment is designed, the concentration of dissolved oxygen in a water body can be controlled to be at a higher level, and the influence caused by insufficient temperature is compensated by high dissolved oxygen.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: provides a low-temperature bacteria proportioning culture method for sewage treatment, which solves the problems that the activity of microorganisms is reduced, the sludge bulking phenomenon can occur and the sewage treatment effect is influenced under the low-temperature condition of the existing sewage treatment equipment.
The technical problem to be solved by the invention is realized by adopting the following technical scheme:
a low-temperature bacterium proportioning culture method for sewage treatment comprises the following steps:
(1) building sewage treatment equipment, wherein the sewage treatment equipment comprises a regulating tank, an integrated sewage treatment device and a disinfection unit, the integrated sewage treatment device is sequentially provided with an anoxic zone, an aerobic zone and a sedimentation zone along the sewage treatment flow direction, the regulating tank is connected to the bottom of the anoxic zone through a water pipe and a water suction pump, the anoxic zone overflows and is connected to the bottom of the aerobic zone, the aerobic zone overflows and is connected to the bottom of the sedimentation zone, and supernatant of the sedimentation zone is connected to the disinfection unit; the anoxic zone and the aerobic zone are both provided with fillers for storing the microorganism sludge;
(2) firstly adding strains, namely increasing the dissolved oxygen in an aerobic zone to be more than 6mg/L, then adding 28-35g of aerobic strains in each cubic meter of the aerobic zone, activating in a stuffy aeration mode, keeping the dissolved oxygen concentration to be more than 6mg/L, and properly feeding water according to the biological condition in the aerobic zone;
(3) adding strains for the second time, after culturing for 2-4 days, adding 6-9g of aerobic strains in each cubic meter of aerobic area, simultaneously supplementing a proper amount of nitrogen source, and continuing to perform closed aeration for 2-4 days under the condition of maintaining the dissolved oxygen concentration to be more than 6 mg/L;
(4) adding photosynthetic bacteria stock solution, adding 1.5-2kg of photosynthetic bacteria stock solution into the aerobic zone at the final stage, and adding the photosynthetic bacteria stock solution after dilution.
As a preferred example, the liquid on the upper layer of the aerobic zone in the step (1) is connected to the anoxic zone through a water pump and a nitrifying liquid return pipe.
As a preferred example, 32g of aerobic bacterial seeds are fed into each cubic meter of aerobic zone in the step (2).
As a preferable example, 8g of aerobic bacterial seeds are added in each cubic meter of aerobic zone in the step (3).
As a preferable example, 1.87kg of photosynthetic bacteria stock solution is added into the aerobic zone in the step (4).
The scheme takes AO water treatment process equipment as a culture environment, an AO water treatment process (AO is an abbreviation of anaerobic Oxic) is also called an anaerobic-aerobic process method, and A (anaerobic) is an anaerobic section and is used for removing nitrogen and phosphorus; o (oxic) is an aerobic section for removing organic matter from water. It mainly includes equalizing basin (district), oxygen deficiency pond (district), good oxygen pond (district), sedimentation tank (district), disinfection unit:
a regulating pool: domestic sewage is treated by a grid and then enters a regulating tank for regulating and homogenizing water quantity and water quality, the balance and stability of the water quantity and the water quality of a subsequent biochemical treatment system are ensured, and a pre-aeration system is arranged for oxygenation and stirring to prevent suspended particles in the sewage from precipitating and smelling, and also plays a certain role in degrading organic matters in the sewage, so that the impact resistance and the treatment effect of the whole system are improved;
an anoxic tank: the sewage is further mixed, the high-efficiency biological elastic filler in the pool is fully utilized as a bacterial carrier, indissolvable organic matters in the sewage are converted into soluble organic matters by facultative microorganisms, macromolecular organic matters are hydrolyzed into micromolecular organic matters so as to be beneficial to further oxidative decomposition of a subsequent aerobic pool, and partial nitrification and denitrification can be carried out under the action of nitrobacteria through the returned nitrocarbon nitrogen to remove ammonia nitrogen;
an oxidation pond: the pool is a core part of the sewage treatment and is divided into two sections, wherein the former section is under higher organic load, and various organic substances in the sewage are removed through the biochemical degradation and adsorption action of a large number of microbial communities of different species attached to the filler under the joint participation, so that the content of the organic substances in the sewage is greatly reduced; under the condition of lower organic load, the later stage degrades ammonia nitrogen in the sewage under the condition of sufficient oxygen content through the action of nitrobacteria, and simultaneously reduces the COD value in the sewage to a lower level, so that the sewage is purified;
a sedimentation tank: carrying out solid-liquid separation to remove suspended sludge peeled off from the biochemical tank so as to really purify the sewage;
a disinfection unit: the effluent flows into a disinfection tank for disinfection, so that the effluent quality meets the requirement of sanitary index and is discharged out in a qualified mode.
The invention has the beneficial effects that: the reasonable strain proportion and the proper activation and domestication method generate high-efficiency and low-temperature-resistant low-temperature strains; the low-temperature strain has higher treatment efficiency for treating domestic sewage in a low-temperature environment, and compared with a common heat preservation or heating method, the energy consumption cost is much lower; the low-temperature bacterium proportioning culture method can solve the problem of sludge bulking caused by low temperature; the combination of different strains can more effectively strengthen the denitrification and COD degradation performance of the system.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
In the figure: the device comprises a regulating tank 1, an integrated sewage treatment device 2, a disinfection unit 3, an anoxic zone 4, an aerobic zone 5, a settling zone 6 and a nitrifying liquid return pipe 7.
Detailed Description
In order to make the technical means, the original characteristics, the achieved purpose and the efficacy of the invention easy to understand, the invention is further described with reference to the specific drawings.
Examples
As shown in figure 1, a low-temperature bacteria ratio culture method for sewage treatment comprises the following steps:
(1) building sewage treatment equipment, wherein the sewage treatment equipment comprises an adjusting tank 1, an integrated sewage treatment device 2 and a disinfection unit 3, the integrated sewage treatment device 2 is sequentially provided with an anoxic zone 4, an aerobic zone 5 and a sedimentation zone 6 along the sewage treatment flow direction, the adjusting tank 1 is connected to the bottom of the anoxic zone 4 through a water pipe and a water suction pump, the anoxic zone 4 overflows and is connected to the bottom of the aerobic zone 5, the aerobic zone 5 overflows and is connected to the bottom of the sedimentation zone 6, supernatant of the sedimentation zone 6 is connected to the disinfection unit 3, and supernatant of the aerobic zone 5 is connected to the anoxic zone 4 through a water pump and a nitrification liquid return pipe 7;
(2) firstly adding strains, namely increasing the dissolved oxygen in the aerobic zone 5 to be more than 6mg/L, then adding 32g of aerobic strains in each cubic meter of the aerobic zone, activating in a stuffy aeration mode, keeping the dissolved oxygen concentration to be more than 6mg/L, and properly feeding water according to the biological condition in the aerobic zone 5;
(3) adding strains for the second time, after culturing for 3 days, adding 8g of aerobic strains into each cubic meter of aerobic zone according to 5g of aerobic strains, simultaneously supplementing a proper amount of nitrogen source, and continuing to perform closed aeration for 3 days under the condition of maintaining the dissolved oxygen concentration to be more than 6 mg/L;
(4) adding a photosynthetic bacteria stock solution, adding 1.87kg of photosynthetic bacteria stock solution into the aerobic zone 5 at the final stage, and adding the photosynthetic bacteria stock solution after dilution.
In the step (2) and the step (3), the aerobic strains adopt commercially available composite strains with common brands of rich sweetness, sweetness and the like, the growth and propagation characteristics of microorganisms such as bacillus, lactic acid bacteria, saccharomycetes and nitrobacteria are combined, the methods such as microorganism activation, domestication, culture and the like are improved and optimized, the living environment of the microorganisms is improved by high dissolved oxygen, and the activity of the microorganisms is improved, so that the adverse effect of temperature on the sewage treatment effect is practically and effectively solved. The specific adding mode is that the strains are added in one time by adopting the sweet fungus, and the strains are added in the second time by adopting the sweet fungus.
Among them, photosynthetic bacteria (Photosynthetlc bacteria) are a general term for bacteria that do not release oxygen and perform photosynthesis in anaerobic environment, wherein some strains of rhodospirillaceae can rapidly decompose low molecular organic matters under anaerobic illumination or aerobic dark conditions. Therefore, different strains are domesticated and cultured in a proper proportion mode to make up for the influence of low temperature on the process treatment effect.
The composite strains are cultured according to a certain number of times and proportion, the concentration of the dissolved oxygen in the water body is controlled to be at a higher level, the problem of unsatisfactory process treatment effect caused by insufficient temperature is solved through high dissolved oxygen, and the method is applied to the buried intensive integrated sewage treatment device 2 to improve the treatment performance of the device.
The reasonable strain proportion and the proper activation and domestication method generate high-efficiency and low-temperature-resistant low-temperature strains; the low-temperature strain has higher treatment efficiency for treating domestic sewage in a low-temperature environment, and compared with a common heat preservation or heating method, the energy consumption cost is much lower; the low-temperature bacterium proportioning culture method can solve the problem of sludge bulking caused by low temperature; the combination of different strains can more effectively strengthen the denitrification and COD degradation performance of the system.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (5)
1. A low-temperature bacterium proportioning culture method for sewage treatment is characterized by comprising the following steps:
(1) building sewage treatment equipment, wherein the sewage treatment equipment comprises a regulating tank, an integrated sewage treatment device and a disinfection unit, the integrated sewage treatment device is sequentially provided with an anoxic zone, an aerobic zone and a sedimentation zone along the sewage treatment flow direction, the regulating tank is connected to the bottom of the anoxic zone through a water pipe and a water suction pump, the anoxic zone overflows and is connected to the bottom of the aerobic zone, the aerobic zone overflows and is connected to the bottom of the sedimentation zone, and supernatant of the sedimentation zone is connected to the disinfection unit;
(2) firstly adding strains, namely increasing the dissolved oxygen in an aerobic zone to be more than 6mg/L, then adding 28-35g of aerobic strains in each cubic meter of the aerobic zone, activating in a stuffy aeration mode, keeping the dissolved oxygen concentration to be more than 6mg/L, and properly feeding water according to the biological condition in the aerobic zone;
(3) adding strains for the second time, after culturing for 2-4 days, adding 6-9g of aerobic strains in each cubic meter of aerobic area, simultaneously supplementing a proper amount of nitrogen source, and continuing to perform closed aeration for 2-4 days under the condition of maintaining the dissolved oxygen concentration to be more than 6 mg/L;
(4) adding photosynthetic bacteria stock solution, adding 1.5-2kg of photosynthetic bacteria stock solution into the aerobic zone at the final stage, and adding the photosynthetic bacteria stock solution after dilution.
2. The method for culturing the low-temperature bacteria according to the claim 1, wherein the liquid on the upper layer of the aerobic zone in the step (1) is connected to the anoxic zone through a water pump and a nitrifying liquid return pipe.
3. The method for culturing the low-temperature bacteria according to the claim 1, wherein 32g of aerobic bacteria are added to each cubic meter of aerobic zone in the step (2).
4. The method for culturing the low-temperature bacteria according to the claim 1, wherein 8g of aerobic bacteria are added in each cubic meter of aerobic zone in the step (3).
5. The method for culturing the low-temperature bacteria in proportion for sewage treatment as claimed in claim 1, wherein 1.87kg of raw solution of photosynthetic bacteria is added into the aerobic zone in the step (4).
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114163074A (en) * | 2021-12-03 | 2022-03-11 | 南京大学 | Method for treating tail water of sewage treatment plant by adopting constructed wetland |
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2020
- 2020-05-20 CN CN202010428292.4A patent/CN111606509A/en active Pending
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Application publication date: 20200901 |