CN110407336B - Wet storage and activity recovery method based on MBBR (moving bed biofilm reactor) autotrophic denitrification suspension carrier - Google Patents
Wet storage and activity recovery method based on MBBR (moving bed biofilm reactor) autotrophic denitrification suspension carrier Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/301—Aerobic and anaerobic treatment in the same reactor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
- C02F3/307—Nitrification and denitrification treatment characterised by direct conversion of nitrite to molecular nitrogen, e.g. by using the Anammox process
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/02—Temperature
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/14—NH3-N
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/16—Total nitrogen (tkN-N)
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/22—O2
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Abstract
The invention discloses a wet preservation and activity recovery method based on an MBBR autotrophic nitrogen removal suspended carrier, belonging to the technical field of biological nitrogen removal of sewage. The method solves the technical problems of complex preservation environment, slow activity recovery, poor treatment effect and the like in the prior art. The storage method comprises the following steps: stopping running the reactor containing the MBBR-based autotrophic denitrification suspension carrier, normally opening a water outlet of the reactor, spraying nutrient solution to the suspension carrier every 2-4 days, and maintaining room temperature for storage in a later period, wherein the method for restoring the activity after storage comprises the following steps: nutrient solution is introduced into the reactor, the SBR operation is recovered to 30-34 ℃, then continuous flow operation is recovered, and the water inlet flow and the aeration quantity are slowly increased to the level before preservation, so that the functional microbial activity can be completely recovered. The invention can successfully store the autotrophic nitrogen removal suspended carrier for a long time, has quick activity recovery and can completely recover the treatment effect of the autotrophic nitrogen removal reactor.
Description
Technical Field
The invention relates to the technical field of biological denitrification of sewage, in particular to a preservation and activity recovery method based on an MBBR autotrophic denitrification suspended carrier.
Background
The traditional nitrification and denitrification process needs a large amount of aeration, carbon source addition and energy consumption, the discovery of anaerobic ammonia oxidation provides a new metabolic pathway for the denitrification process, autotrophic denitrification based on the anaerobic ammonia oxidation process is considered to be one of the denitrification processes with the most development prospect, and the process couples short-cut nitrification and anaerobic ammonia oxidation, and compared with the traditional nitrification and denitrification process, the process has the advantages of saving 60% of aeration amount, not needing to add organic carbon source, reducing 90% of sludge yield, relatively reducing the release amount of nitrogen oxides and the like. The MBBR technology comprises the steps of adding a suspension carrier into an autotrophic nitrogen removal system, and enabling two functional microorganisms of autotrophic nitrogen removal to be respectively arranged on an outer layer and an inner layer of a biological membrane, wherein the outer layer of the suspension carrier forms an aerobic biological membrane taking Ammonia Oxidizing Bacteria (AOB) as dominant bacteria, and the inner layer forms an anaerobic/anoxic biological membrane mainly taking anaerobic ammonia oxidizing bacteria (ANAOB). Therefore, a series of defects that the traditional activated sludge method has low tolerance to high dissolved oxygen, needs to be provided with a secondary sedimentation tank to prevent strain loss, is difficult to realize the synergy between the nitrification performance of flocculent sludge and the anaerobic ammonia oxidation performance of granular sludge and the like are overcome, and the CANON form with the most engineering potential is formed.
Since anammox bacteria (AnAOB), one of CANON-functional microorganisms, grow slowly and the doubling time is usually 7-29d, the start-up of the autotrophic denitrification process is limited, and to overcome the economic disadvantage, the start-up time of the reactor is usually shortened by inoculating mature autotrophic denitrification microorganism sources. Therefore, the storage method of the seed source and the rapid recovery of the functional microbial activity after the storage are very important for the engineering application of the autotrophic nitrogen removal process. Regarding the storage method, the current research mostly focuses on the granular sludge type reactor, the focus is mostly on the storage temperature, the substrate concentration and the storage time, and the common problems are that the storage method is complex and the activity recovery effect is poor.
The research reports of the related aspects of the prior art mainly include:
CN 103755109A provides a method for preserving anaerobic ammonium oxidation granular sludge, which comprises the following steps: collecting anaerobic ammonium oxidation granular sludge to be preserved, cleaning the anaerobic ammonium oxidation granular sludge for 3-5 times by using an inorganic salt buffer solution before preservation, selecting a clean and well-sealed organic plastic bottle as a preservation container, adding clean water and dimethyl sulfoxide into the preservation container, putting the preservation container into an environment with the temperature of 0-4 ℃ for refrigeration for 3-6h, transferring the cleaned sludge into the refrigerated sealed container, aerating for 20-30min by argon gas, and then putting the sealed container into the environment with the temperature of 0-4 ℃ for preservation. The reactivation method after storage is as follows: taking out the preservation container, firstly carrying out a temperature adaptation test before activity recovery, after temperature adaptation, washing the preserved granular sludge for 6-10 times by using the inorganic salt buffer solution, and then transferring the granular sludge into an anaerobic reactor to be started for activity recovery. The invention relates to a method for preserving and reactivating autotrophic nitrogen removal functional microorganisms AnAOB, which is characterized in that in the preservation method, a pre-prepared inorganic salt buffer solution, clean water and dimethyl sulfoxide are required, a preservation container is required to be cooled, and the whole preservation environment needs 0-4 ℃, so that the whole preservation process is complicated and tedious, generally, the AnAOB can be in an advantage state when the abundance of the functional microorganisms AnAOB in an autotrophic nitrogen removal reactor reaches more than 20%, and the growth of other miscellaneous bacteria can be inhibited, so that the complicated preservation step is not necessary.
CN 102336505 a discloses a method for preserving anaerobic ammonium oxidation granular sludge, which comprises: storing sludge to be preserved in a closed container, keeping the closed container in an anoxic state, and placing at normal temperature with pH controlled at 7.0-9.0; nutrient solution and fresh anammox granular sludge are added into the container at intervals to ensure the anammox activity of the preserved sludge. The method for preserving the autotrophic nitrogen removal functional microorganism AnAOB needs to continuously add fresh anaerobic ammonium oxidation granular sludge in the preservation process, needs a large amount of anaerobic ammonium oxidation strains in the whole preservation process, and has overhigh cost.
The effect of plum blossom (Li Xiang, Zheng Yuhui, Huang Yong, etc..) on the activity of the anaerobic ammonia oxidation sludge by the storage temperature and the storage time [ D ], 2011.) researches the change rule of the activity of the anaerobic ammonia oxidation sludge of the autotrophic denitrification functional microorganism and the recovery capability of the sludge activity. The result shows that the preservation temperature and the preservation time have obvious influence on the activity of the anaerobic ammonium oxidation sludge. The normal temperature (15 +/-2) DEG C and the low temperature (5 +/-2) DEG C have small influence on the activity of the anaerobic ammonia oxidation sludge, and the medium temperature (30 +/-2) DEG C and the freezing temperature (20 +/-2) DEG C can greatly reduce or even disappear the activity of the anaerobic ammonia oxidation sludge. The anammox sludge activity decreased rapidly and then tended to be slow during the first 30 days of storage. The attenuation index at normal temperature was estimated to be 0.0324 according to the attenuation index model, and the attenuation index was the smallest for other temperatures. And a proper preservation method is provided according to the characteristics of the influence of the preservation temperature and the preservation time on the anaerobic ammonia oxidation sludge, so that the activity of the anaerobic ammonia oxidation sludge can be recovered in a short time. The research adopts a reactor with the effective volume of 0.1L, is difficult to provide guidance for engineering projects, and in addition, the research is carried out by water distribution, and the complex water quality in the actual engineering projects is lack of data support.
Disclosure of Invention
The invention aims to provide a wet storage and activity recovery method based on an MBBR (moving bed biofilm reactor) autotrophic denitrification suspension carrier, which can ensure that the MBBR autotrophic denitrification suspension carrier is stored for a long time and the activity can be completely recovered.
One of the tasks of the invention is to provide a wet preservation method based on an MBBR autotrophic denitrification suspension carrier, which comprises the following steps:
a. stopping the operation of the reactor containing the MBBR-based autotrophic denitrification suspension carrier, and closing related equipment;
b. opening a water outlet of the reactor to discharge the mixed liquid, and storing the autotrophic denitrification suspended carrier in a dark place at normal temperature;
c. and c, spraying nutrient solution into the reactor from which the mixed solution is discharged in the step b every 2-4 days, and keeping a water outlet of the autotrophic denitrification reactor in an open state.
Preferably, the density of the suspension carrier based on MBBR autotrophic denitrification is 0.985-1.005g/cm3Effective specific surface area of 450m or more2/m3The suspended carrier is grown with a biological film, and ammonia oxidizing bacteria and anaerobic ammonia oxidizing bacteria exist in a layered mode in a biological film mode, so that the suspended carrier has an autotrophic nitrogen removal effect and TN removal volume load is more than or equal to 1.0 kgN/(m)3·d)。
Preferably, the relevant equipment of step a comprises a water inlet device, an aeration device, a heating device and a stirring device.
Further preferably, the ammonia nitrogen concentration of the nutrient solution in the step c is 100-250 mg/L.
More preferably, the room temperature in step e is greater than 4 ℃ and not greater than 30 ℃.
The invention discloses a wet preservation method based on an MBBR autotrophic nitrogen removal suspended carrier, which has the following principle:
when the nutrition of the autotrophic denitrification microorganisms is deficient in the surrounding environment, the autotrophic denitrification microorganisms can degrade EPS generated by the autotrophic denitrification microorganisms to maintain slow growth and metabolism, the mixed liquid in the autotrophic denitrification reactor is discharged, so that the surrounding environment is in a nutrition deficient state, the life activities of the autotrophic denitrification microorganisms are reduced, and meanwhile, the autotrophic denitrification suspension carriers in the reactor are regularly sprayed with nutrient solution, so that the aim of maintaining the normal shape of cells is fulfilled, the bacterial cells are prevented from being dried for a long time and losing water, and in addition, the activity of the autotrophic denitrification microorganisms can be maintained to a certain degree and the autotrophic denitrification microorganisms can be kept secreting the EPS for self utilization. Meanwhile, the autotrophic denitrification reactor is provided with a light screen and keeps the system in an anoxic state so as to protect the life activities of the anaerobic ammonium oxidation bacteria from being damaged and avoid the autotrophic denitrification microorganisms from being in an anaerobic environment to cause the decomposition of the bacteria. The preserving method of the invention can preserve the autotrophic denitrification microorganisms for a long time.
The invention also aims to provide an activity recovery method of the suspension carrier obtained by the preservation method of the MBBR-based autotrophic nitrogen removal suspension carrier, which sequentially comprises the following steps:
firstly, filling nutrient solution into a preserved reactor containing the MBBR autotrophic denitrification suspended carrier, and respectively opening a heating device and an aeration device of the reactor filled with the nutrient solution;
secondly, the reactor is in an SBR operation mode, the aeration quantity of the aeration device is controlled to ensure that the dissolved oxygen of the reactor is 0.3-0.6mg/L, and the reactor is operated to the temperature which is recovered to the normal operation temperature of the reactor before the autotrophic denitrification suspended carrier is stored;
thirdly, when the ammonia nitrogen concentration in the reactor is reduced to 30-50mg/L, the operation mode of the reactor is recovered to continuous flow operation, the reactor continuously feeds water and continuously discharges water, and the water feeding adopts water feeding before the reactor stops operating;
fourthly, the water inlet flow of the reactor is adjusted upwards, the up-regulation range is 20% of the water flow before the reactor stops operating, 2 HRTs are operated when the water inlet flow is adjusted upwards once, 10 HRTs are operated in the whole recovery process, and the water inlet flow is adjusted upwards five times;
fifthly, the aeration rate of the reactor is required to be adjusted up after the water inflow rate is adjusted up every time, the aeration rate is adjusted according to the dissolved oxygen in the reactor, and the water inflow rate is required to be adjusted up 5 times while the dissolved oxygen in the reactor is maintained to be respectively: more than 0.8 and less than 1.2mg/L, more than or equal to 1.2 and less than 1.5mg/L, more than or equal to 1.5 and less than 1.8mg/L, more than or equal to 1.8 and less than 2.1mg/L, more than or equal to 2.1 and less than 2.4 mg/L; sixthly, when the volume load of TN removal of the reactor reaches more than 95% before the operation is stopped, the reactor enters into stable operation.
The invention discloses an MBBR-based autotrophic nitrogen removal suspended load activity recovery method, which has the following principle:
in an MBBR-based autotrophic denitrification reactor, biofilms are mainly retained in the reactor by EPS-forming aggregates secreted by the microorganisms attached to a suspension carrier. Under the condition of rich nutrition, EPS can adsorb a large amount of nutrient substances so as to maintain life activities under the condition of nutrient deficiency. In the process of storing the autotrophic denitrification suspended carrier in the reactor, functional microorganisms in the biomembrane enter an endogenous respiration period after being stored for a long time without a matrix, and begin to degrade EPS secreted by the microorganisms to maintain life activities, so that PN and PS components of TB-EPS and LB-EPS of the biomembrane are reduced, and the reduction of the EPS causes the adhesion performance of the biomembrane to be reduced to a certain extent. The temperature is recovered firstly, so that the microbial activity can be recovered, the EPS can be secreted, the adhesion performance of the biological membrane can be enhanced, and the functional microorganisms can be adapted in time by gradually increasing the water inflow and the aeration amount, so that the influence on the functional microorganisms is minimized, and the recovery of the treatment effect of the reactor is facilitated.
Compared with the prior art, the invention has the following beneficial technical effects:
1) the preservation method is simple, only the mixed liquid of the reactor needs to be emptied during preservation, and the reactor is simply shaded, so that the preservation difficulty is low;
2) the energy is saved, the consumption is reduced, the preparation method of the nutrient solution sprayed by the port with fixed time is simple during storage, no special medicament is used, no special requirement on temperature during storage is required, and the cost is saved;
3) the activity is recovered well, the autotrophic nitrogen removal is in an MBBR form, autotrophic nitrogen removal microorganisms can be efficiently enriched, the EPS secretion of the biomembrane is higher than that of activated sludge, the autotrophic nitrogen removal microorganisms can be maintained in a nutrient-deficient state for a long time, and the declaration activity is ensured.
4) The operation stability is strong, and based on the alternate operation of the high ammonia nitrogen wastewater and the mainstream sewage, the high ammonia nitrogen environment is intermittently provided for the anammox bacteria, the functional microorganisms can be rapidly enriched, and the retention of the anammox bacteria is ensured not to be degraded.
Detailed Description
The invention provides a method for preserving in a moist manner and recovering activity based on an MBBR autotrophic denitrification suspension carrier, and in order to make the advantages and technical scheme of the invention clearer and clearer, the invention is explained in detail with reference to specific embodiments.
First, the related art terms involved in the present invention are explained as follows:
1) autotrophic nitrogen removal: it is a general term for nitrosation and anaerobic ammonia oxidation (ANAMMOX), thereby achieving the purpose of denitrification; under aerobic conditions, nitrite bacteria (AOB) oxidize ammonia nitrogen part into nitrous acid, and the produced nitrous acid and part of the rest ammonia nitrogen are subjected to anaerobic ammonia oxidation (ANAMMOX) reaction under the action of anaerobic ammonia oxidation bacteria (ANAOB) to generate nitrogen;
2) HRT: the hydraulic retention time refers to the average retention time of the water to be treated in the reactor, namely the time required by complete water change when the autotrophic denitrification reactor runs at a certain inflow rate, and h; if the water changing period of the reactor is equal to the effective volume of the reactor and divided by the water inlet flow of the reactor;
3) TN removal of volume load: the mass of TN removed per unit volume of reaction cell per day, kgN/(m)3D); if the removal volume load from the first autotrophic denitrification pool to the aerobic nitrification pool TN is equal to (the water inlet TN of the first autotrophic denitrification pool TN-the water outlet TN of the aerobic nitrification pool) + the total pool volume from the first autotrophic denitrification pool to the aerobic nitrification pool TN/the daily water inlet volume of the reaction pool;
4) EPS: extracellular Polymers (EPS) are some high molecular polymers secreted in vitro by microorganisms, mainly bacteria, under certain environmental conditions. The main components are similar to the intracellular components of the microorganism, and are some high molecular substances, such as polysaccharide, protein, nucleic acid and other polymers.
5) The "reactor" referred to herein is an "autotrophic denitrification reactor".
Next, the MBBR-based autotrophic nitrogen removal reactor related to the method for preserving and restoring the activity of the MBBR-based autotrophic nitrogen removal suspension carrier by wetting will be described in detail.
The reactor containing the MBBR-based autotrophic denitrification suspension carrier mainly structurally comprises the following components:
the flow state of the mixed liquid in the reactor is an up-flow type, the bottom of the reactor is provided with a water inlet and a main water inlet pipeline,the upper part is provided with a water outlet and a main water outlet pipeline, an intercepting screen is arranged in front of the water outlet, the bottom of the reactor is respectively provided with a perforated aeration pipe and a microporous aeration pipe, wherein the opening direction of the perforated aeration pipe is downward, the aperture of the opening is 4-6mm, the installation height is 30cm above the bottom of the reactor, the microporous aeration pipe is aerated by a microporous aeration disc or a microporous aeration head, and the opening of the aeration disc or the aeration head is upward. A heating device is arranged in the reactor, and the heating device is a heating rod with the power of 6 kw. The reactor is provided with a stirring device, the stirring device adopts a spiral three-blade stirrer, and the power of the stirrer is 15-50W/m3And (4) selecting the type.
The following is a detailed description of the method for preserving the suspended autotrophic nitrogen removal carrier based on MBBR in the present invention.
A wet preservation method of an MBBR-based autotrophic nitrogen removal suspension carrier specifically comprises the following steps:
a. stopping running the reactor containing the MBBR-based autotrophic denitrification suspension carrier, closing related equipment, and reducing the activity of autotrophic denitrification microorganisms in the reaction tank;
b. opening a water outlet of the reactor to discharge mixed liquor in the reactor, so that the MBBR-based autotrophic nitrogen removal suspended carrier in the system is only kept in a wet state, avoiding cell water absorption and rupture of autotrophic nitrogen removal microorganisms due to low concentration of surrounding mixed liquor matrix and inorganic salt caused by long-time soaking, storing the autotrophic nitrogen removal suspended carrier in a dark place and at normal temperature, ensuring that the system is in an anoxic state, maintaining the life activity of the autotrophic nitrogen removal functional microorganisms, avoiding inhibition of the autotrophic nitrogen removal functional microorganisms ANAOB activity caused by aerobic conditions, and also preventing the autotrophic nitrogen removal microorganisms from being decomposed caused by anaerobic conditions, wherein the ANAOB is a light-sensitive bacterium, and the secretion of autotrophic nitrogen removal related biological enzymes can be inhibited by light irradiation, so that the system is stored in a dark place;
c. spraying nutrient solution into the autotrophic nitrogen removal reactor discharged by the mixed solution in the step b every 2-4 days, and keeping a water discharge port of the autotrophic nitrogen removal reactor in an open state so as to maintain the normal shape of autotrophic nitrogen removal microorganism cells and prevent the cells from losing water;
further preferably, the related equipment in step a comprises a water inlet device, an aeration device, a heating device and a stirring device.
Further preferably, the ammonia nitrogen concentration of the nutrient solution in the step c is 100-250mg/L, and the alkalinity/ammonia nitrogen is more than or equal to 4.4.
The activity recovery method of the suspension carrier preserved by the preservation method comprises the following specific steps:
filling nutrient solution into a preserved autotrophic nitrogen removal reactor containing an MBBR-based autotrophic nitrogen removal suspension carrier; respectively opening a heating device and an aeration device of the autotrophic denitrification reactor filled with nutrient solution;
and secondly, controlling the aeration quantity of the aeration device to ensure that the dissolved oxygen of the reactor is 0.3-0.6mg/L, and operating to the temperature which is recovered to the normal operating temperature of the reactor before the autotrophic denitrification suspended carrier is stored, namely operating to the normal temperature of the reactor if the reactor is operated at normal temperature, and operating to the high temperature of the reactor if the reactor is operated at high temperature. If the autotrophic denitrification reactor is operated at a high inflow rate under the condition of not recovering the temperature, CANON functional microorganisms cannot be fully adapted and exert normal activity, high free ammonia of the system is caused to continuously exist, normal growth of the CANON functional microorganisms is inhibited, certain toxic action is caused to the CANON functional microorganisms, EPS secretion cannot be recovered in time, and obvious biofilm falling phenomenon is easy to occur under aeration disturbance, so that the load of the reactor is reduced.
Thirdly, when the ammonia nitrogen concentration in the reactor is reduced to 30-50mg/L, the operation mode of the reactor is recovered to continuous flow operation, the reactor continuously feeds water and continuously discharges water, and the water feeding adopts water feeding before the reactor stops operating;
fourthly, the water inlet flow of the reactor is adjusted upwards, the up-regulation range is 20% of the water flow before the reactor stops operating, 2 HRTs are operated when the water inlet flow is adjusted upwards once, 10 HRTs are operated in the whole recovery process, and the water inlet flow is adjusted upwards five times;
fifthly, the aeration rate of the reactor is required to be adjusted up after the water inflow is adjusted up every time, the aeration rate is adjusted according to the dissolved oxygen in the reactor, and the water inflow is required to be maintained for 5 times while the dissolved oxygen in the reactor is respectively: more than 0.8 and less than 1.2mg/L, more than or equal to 1.2 and less than 1.5mg/L, more than or equal to 1.5 and less than 1.8mg/L, more than or equal to 1.8 and less than 2.1mg/L, more than or equal to 2.1 and less than 2.4 mg/L;
and sixthly, when the volume load of TN removal of the reactor reaches more than 95% before the operation is stopped, the reactor enters into stable operation.
The invention preferably selects the nutrient solution to be formed by mixing tap water and high ammonia nitrogen sewage, wherein the ammonia nitrogen concentration is 100-250 mg/L.
To facilitate an understanding of the subject innovation of the present application, the present invention is further described below with reference to specific embodiments.
Example 1:
the autotrophic nitrogen removal project of a certain sewage treatment plant mainly treats sludge digestive juice and designs the water quantity to be 200m3D, COD, BOD of the influent water5、NH3TN design values are respectively 420, 190, 450 and 490mg/L, the MBBR-based autotrophic denitrification suspension carrier is adopted for treatment, the filling rate of the suspension carrier is 50 percent, and the total nitrogen removal volume load can reach 1.4 kgN/(m)3D), the ammonia nitrogen of the effluent of the system can be reduced to about 30mg/L and about TN 65mg/L, and the treatment effect is stable. And (3) in a certain period of time, the sludge digestion tank of the sewage treatment plant in which the project is positioned is refitted to cause that the system does not have nitrifying liquid available, and the autotrophic nitrogen removal reactor stops running at the period of time.
The preservation method of the suspension carrier comprises the following steps: emptying the mixed liquid of the autotrophic nitrogen removal reactor, placing the suspended carrier in the autotrophic nitrogen removal reactor, preparing the nitrifying liquid and tap water which are stored in the early stage according to a ratio of 1:2, periodically spraying the nitrifying liquid and the tap water into the autotrophic nitrogen removal reactor, covering a light shielding plate on the upper part of the autotrophic nitrogen removal reactor, maintaining the temperature in the autotrophic nitrogen removal reactor at 19-22 ℃, and recovering the operation after storing for about 60 days.
Firstly, filling a nutrient solution into a preserved autotrophic nitrogen removal reactor containing an MBBR-based autotrophic nitrogen removal suspended carrier, wherein the sum of the volume of the nutrient solution and the volume of the suspended carrier is consistent with the effective volume of the autotrophic nitrogen removal reactor; secondly, heating and aerating the autotrophic denitrification reactor, operating the autotrophic denitrification reactor SBR, controlling the aeration amount to ensure that the dissolved oxygen of the autotrophic denitrification reactor is 0.4-0.5mg/L, and operating for 2 water changing periods until the temperature is restored to 31 ℃; the water inlet flow of the reactor is adjusted up, the up-adjusting range is 20% of the water flow before the reactor stops operating, 2 HRTs are operated when the water inlet flow is adjusted up once, 10 HRTs are operated in the whole recovery process, and the water inlet flow is adjusted up five times; the aeration rate of the reactor is required to be adjusted up after the water inflow rate is adjusted up every time, the aeration rate is adjusted according to the dissolved oxygen in the reactor, and the dissolved oxygen in the reactor is required to be maintained simultaneously when the water inflow rate is adjusted up for 5 times: more than 0.8 and less than 1.2mg/L, more than or equal to 1.2 and less than 1.5mg/L, more than or equal to 1.5 and less than 1.8mg/L, more than or equal to 1.8 and less than 2.1mg/L, more than or equal to 2.1 and less than 2.4mg/L, when the volume load of TN removal of the reactor reaches more than 95% before the operation is stopped, the reactor enters into stable operation.
Example 2:
the process parameters are set as follows:
certain pilot plant, the treated water quality is mainly landfill leachate, and the designed water quantity is 300m3D, COD, BOD of the influent water5、NH3TN design values are 1200, 450, 1800 and 1950mg/L respectively, the suspension carrier filling rate is 45 percent and the total nitrogen removal volume load can reach 1.3kgN/m by adopting the MBBR-based autotrophic denitrification suspension carrier for treatment3And d, the ammonia nitrogen of the effluent of the system can be reduced to about 50mg/L and TN 190mg/L, and the treatment effect is stable. And (4) renovating the autotrophic nitrogen removal reactor at a certain period of time, and stopping the operation of the autotrophic nitrogen removal reactor at the period of time.
The preservation method of the suspension carrier comprises the following steps: emptying the mixed liquid of the autotrophic nitrogen removal reactor, placing a suspended carrier in the autotrophic nitrogen removal reactor, preparing the garbage percolate and tap water which are stored in the early stage according to a ratio of 1:9, periodically spraying the garbage percolate and the tap water into the autotrophic nitrogen removal reactor, covering a light shielding plate on the upper part of the autotrophic nitrogen removal reactor, maintaining the temperature in the autotrophic nitrogen removal reactor at 17-19 ℃, and recovering the operation after storing for about 50 days.
Firstly, filling a mixed solution prepared by 1:9 of landfill leachate and tap water into a preserved autotrophic nitrogen removal reactor containing an MBBR-based autotrophic nitrogen removal suspended carrier, wherein the sum of the mixed volume and the volume of the suspended carrier is consistent with the effective volume of the autotrophic nitrogen removal reactor; secondly, heating and aerating the autotrophic denitrification reactor, operating the autotrophic denitrification reactor SBR, controlling the aeration amount to ensure that the dissolved oxygen of the reactor is 0.5-0.6mg/L, and operating for 1 water changing period until the temperature is recovered to 32 ℃; the water inlet flow of the reactor is adjusted up, the up-adjusting range is 20% of the water flow before the reactor stops operating, 2 HRTs are operated when the water inlet flow is adjusted up once, 10 HRTs are operated in the whole recovery process, and the water inlet flow is adjusted up five times; the aeration rate of the reactor is required to be adjusted up after the water inflow rate is adjusted up every time, the aeration rate is adjusted according to the dissolved oxygen in the reactor, and the dissolved oxygen in the reactor is required to be maintained simultaneously when the water inflow rate is adjusted up for 5 times: more than 0.8 and less than 1.2mg/L, more than or equal to 1.2 and less than 1.5mg/L, more than or equal to 1.5 and less than 1.8mg/L, more than or equal to 1.8 and less than 2.1mg/L, more than or equal to 2.1 and less than 2.4mg/L, when the volume load of TN removal of the reactor reaches more than 95% before the operation is stopped, the reactor enters into stable operation.
The parts which are not described in the invention can be realized by taking the prior art as reference.
It should be understood that equivalents may be substituted by those skilled in the art, given the benefit of this disclosure, which are intended to fall within the scope of the invention.
Claims (5)
1. A wet preservation method based on an MBBR autotrophic denitrification suspension carrier is characterized by sequentially comprising the following steps:
a. stopping the operation of the reactor containing the MBBR-based autotrophic denitrification suspension carrier, and closing related equipment;
b. opening a water outlet of the reactor to discharge the mixed liquid, and storing the autotrophic denitrification suspended carrier in a dark place at normal temperature;
c. spraying nutrient solution into the reactor from which the mixed solution is discharged in the step b every 2-4 days, and keeping a water outlet of the autotrophic denitrification reactor in an open state;
the density of the suspended carrier based on the MBBR autotrophic denitrification is 0.985-1.005g/cm3Effective specific surface area of 450m or more2/m3The suspended carrier is grown with biological membrane and ammoniaThe oxidizing bacteria and the anaerobic ammonium oxidizing bacteria exist in a biofilm mode in a layered mode, so that the suspended carrier has an autotrophic denitrification effect, and TN removal volume load is more than or equal to 1.0 kgN/(m)3·d)。
2. The MBBR autotrophic denitrification suspension carrier-based wet storage method according to claim 1, wherein: the related equipment in the step a comprises a water inlet device, an aeration device, a heating device and a stirring device.
3. The MBBR autotrophic denitrification suspension carrier-based wet storage method according to claim 1, wherein: the ammonia nitrogen concentration of the nutrient solution in the step c is 100-250 mg/L.
4. The MBBR autotrophic denitrification suspension carrier-based wet storage method according to claim 1, wherein: the normal temperature in the step b is more than 4 ℃ and less than or equal to 30 ℃.
5. The method for restoring the activity of a suspended carrier obtained by the MBBR autotrophic denitrification suspended carrier-based wetting preservation method according to any one of claims 1-4, wherein the method comprises the following steps in sequence:
firstly, filling nutrient solution into a preserved reactor containing the MBBR autotrophic denitrification suspended carrier, and respectively opening a heating device and an aeration device of the reactor filled with the nutrient solution;
secondly, the reactor is in an SBR operation mode, the aeration quantity of the aeration device is controlled to ensure that the dissolved oxygen of the reactor is 0.3-0.6mg/L, and the reactor is operated to the temperature which is recovered to the normal operation temperature of the reactor before the autotrophic denitrification suspended carrier is stored;
thirdly, when the ammonia nitrogen concentration in the reactor is reduced to 30-50mg/L, the operation mode of the reactor is recovered to continuous flow operation, the reactor continuously feeds water and continuously discharges water, and the water feeding adopts water feeding before the reactor stops operating;
fourthly, the water inlet flow of the reactor is adjusted upwards, the up-regulation range is 20% of the water flow before the reactor stops operating, 2 HRTs are operated when the water inlet flow is adjusted upwards once, 10 HRTs are operated in the whole recovery process, and the water inlet flow is adjusted upwards five times;
fifthly, the aeration rate of the reactor is adjusted up after the water inflow rate is adjusted up every time, the aeration rate is adjusted according to the dissolved oxygen in the reactor, and the dissolved oxygen in the reactor is maintained to be respectively as follows when the water inflow rate is adjusted up for 5 times: more than 0.8 and less than 1.2mg/L, more than or equal to 1.2 and less than 1.5mg/L, more than or equal to 1.5 and less than 1.8mg/L, more than or equal to 1.8 and less than 2.1mg/L, more than or equal to 2.1 and less than 2.4 mg/L;
sixthly, when the volume load of TN removal of the reactor reaches more than 95% before the operation is stopped, the reactor enters into stable operation.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102173551A (en) * | 2011-03-18 | 2011-09-07 | 东北林业大学 | Storage and quick activity recovery method for aerobic granular sludge in normal temperature |
| CN202744430U (en) * | 2012-06-28 | 2013-02-20 | 杭州师范大学 | Anaerobic ammonia oxidation granule sludge storing device |
| CN103755109A (en) * | 2013-12-29 | 2014-04-30 | 杭州师范大学 | Storage and reactivation methods of anaerobic ammonia oxidation granule sludge |
| CN103787561A (en) * | 2014-01-23 | 2014-05-14 | 杭州师范大学 | Application of trehalose in preservation of anaerobic ammonia oxidation granular sludge |
| CN103880168A (en) * | 2014-03-19 | 2014-06-25 | 北京工业大学 | Aerobic nitration granule sludge long-term storage and activity recovery method |
| CN106434350A (en) * | 2016-10-20 | 2017-02-22 | 北京交通大学 | Preservation method of anaerobic ammonium oxidation strain |
| CN106967634A (en) * | 2017-03-27 | 2017-07-21 | 山东大学 | The store method of sequencing batch activated sludge flora |
-
2019
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Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102173551A (en) * | 2011-03-18 | 2011-09-07 | 东北林业大学 | Storage and quick activity recovery method for aerobic granular sludge in normal temperature |
| CN202744430U (en) * | 2012-06-28 | 2013-02-20 | 杭州师范大学 | Anaerobic ammonia oxidation granule sludge storing device |
| CN103755109A (en) * | 2013-12-29 | 2014-04-30 | 杭州师范大学 | Storage and reactivation methods of anaerobic ammonia oxidation granule sludge |
| CN103787561A (en) * | 2014-01-23 | 2014-05-14 | 杭州师范大学 | Application of trehalose in preservation of anaerobic ammonia oxidation granular sludge |
| CN103880168A (en) * | 2014-03-19 | 2014-06-25 | 北京工业大学 | Aerobic nitration granule sludge long-term storage and activity recovery method |
| CN106434350A (en) * | 2016-10-20 | 2017-02-22 | 北京交通大学 | Preservation method of anaerobic ammonium oxidation strain |
| CN106967634A (en) * | 2017-03-27 | 2017-07-21 | 山东大学 | The store method of sequencing batch activated sludge flora |
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