CN1602677A - Low temperature resistant bacteria strain culture method suitable for low temperature daily sewage treatment - Google Patents
Low temperature resistant bacteria strain culture method suitable for low temperature daily sewage treatment Download PDFInfo
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- CN1602677A CN1602677A CN 200410044041 CN200410044041A CN1602677A CN 1602677 A CN1602677 A CN 1602677A CN 200410044041 CN200410044041 CN 200410044041 CN 200410044041 A CN200410044041 A CN 200410044041A CN 1602677 A CN1602677 A CN 1602677A
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Abstract
Be fit for the low temperature effluent disposing the way to cultivate this germ. This invention relates to the method to deal with low temperature effluent, to choose low temperature term and cultivate it with human operation. We accomplish it through: a) when the temperature of effluent is below 15 deg.C, fetch the mud under the examining well to the cultivate reactor. b) Add fresh deject water and the dilute it. Put out the gas change water every day. C) Repeat the b process, after 8 days, the germ mass formed. D) Change the cycle period of the system and change the proportion of nutritional liquor, and then decrease the effluent temperature in vessel. E) Repeat the d process ten times. When the temperature of the water is below 10 deg.C, change the cycle period of the system and change the proportion of nutritional liquor and then decrease the temperature of effluent. Accomplish the germ cultivation. This invention discloses easy to operate and is convenient to choose the materials. All the process could achieve in aquarium and plastic vessels.
Description
Technical field:
The present invention relates to a kind of method that low-temperature sewage is handled, filtered out low temperature resistant bacterial classification and cultivated and tame from occurring in nature that can be applicable under artificial condition.
Background technology:
At present, the sanitary sewage disposal technology that is applied to China adopts activated sludge process mostly, this method is to utilize to carry out metabolism attached to the bacterium on the activated sludge and other microorganisms, utilize organic substance in the sewage as the nutrient source of self growth and breeding, thereby organic pollution materials is removed.The northern China cold district is positioned to the north of 40 ° of the north latitude mostly, and winter climate cold reaches 3～6 months ice period, and the minimum air temperature is generally below-30 ℃, and the average water temperature of sanitary sewage in winter is not higher than 10 ℃, and minimum water temperature can reach 5 ℃.The aeration treating apparatus of sanitary sewage disposal factory adopts open-air the layout usually, there are problems such as quantity is few, metabolic activity is low, generation time is long in low temperature resistant bacterial classification that can degradable organic pollutant in the ubiquity activated sludge during winter operation, and this has directly influenced the effect that sewage treatment plant moves in the winter time.General employing reduces measures such as sludge loading, increase mud capacity of returns, increase dwell time of sewage in engineering at present, some sewage treatment project even will take the way that is incubated or heats up, be used for overcoming the characteristics that water temperature is low, conventional microorganism of using is difficult for survival of sewage, but this can increase construction investment and operating cost undoubtedly, and the effect of handling also is difficult to be guaranteed fully.
Summary of the invention:
The present invention is directed to present the northern area of China sanitary sewage disposal factory winter operation weak effect, water outlet problem not up to standard, a kind of low temperature resistant culture of strains method that is applicable to that low-temperature sewage is handled is provided.The present invention cultivates low temperature resistant bacterial classification like this: a, when the sewage temperature mean value in the sanitary sewage drainage pipeline is lower than 15 ℃, get the bed mud of inspection shaft, bed mud is added in the reactor in the biochemical incubator of low temperature after treatment, carries out vexed exposing to the sun under 15 ℃; B, fresh ight soil water and thin up to the BOD concentration of adding are that 800mg/L, ammonia nitrogen concentration are that 40mg/L, phosphorus concentration are 8mg/L, and regulating the pH value is 7.0, slow aeration, and control DO concentration is 2.0～4.0mg/L, changes water every day 1 time; C, repetition b step, the zoogloea of bacterium forms after 10 days; D, change the proportioning of system's cycle of operation and water inlet nutrient solution, reactor moves 3 cycles every day, and control pH value is 7.0, and regulating aeration rate DO is 2.0mg/L, simultaneously with sewage temperature in average per 2 days 1 ℃ speed reduction reactors; When reducing to 10 ℃, water temperature changes the proportioning of the cycle of operation and water inlet nutrient solution behind e, the 10d once more, reactor moves 2 cycles every day, control pH value is 7.5～8.0, DO is 2.0mg/L, reduce sewage temperature in the reactor gradually with average per 7 days speed of 1 ℃ simultaneously, time water temperature through 5 week is reduced to 5 ℃, finishes culture of strains and domestication.The present invention adopts under artificial condition the method that filters out low temperature resistant bacterial classification (the journal vol.34.No.4 of Harbin Institute of Technology in August, 2002: the separation of Psychrotrophs and in the low-temperature sewage Application on Treatment) and cultivated and tame from occurring in nature, the low temperature resistant efficient composite flora that preparation has greater activity, can be added in the aeration basin when needed, increase effective bacterial classification quantity, thereby overcome the harmful effect of low temperature the biological cleaning effect.Use the resulting low temperature resistant bacterial classification of this method and when water temperature is low, be added in the aeration basin, can make system increase to 75～80% by original 40～45% at 5 ℃ COD clearance; The sludge loading of system can increase to 0.75kgBOD/kgMLSSd by 0.15～0.25kgBOD/kgMLSSd; The initial stage absorption property of activated sludge increases, and initial stage COD clearance can be increased to about 40% by 23.6%.This method is easy and simple to handle, and material selection is convenient, and the process of cultivation and domesticated strain can be carried out in the reactor of being made by lucite and plastics.
Embodiment:
Embodiment one: present embodiment is achieved in that a, when the sewage temperature mean value in the sanitary sewage drainage pipeline is lower than 15 ℃, gets the bed mud of inspection shaft, and bed mud is added to after treatment in the biochemical incubator of low temperature and carries out vexed exposing to the sun under 15 ℃; B, fresh ight soil water and thin up to the BOD concentration of adding are that 800mg/L, ammonia nitrogen concentration are that 40mg/L, phosphorus concentration are 8mg/L, and regulating the pH value is 7.0, slow aeration, and control DO concentration is 2.0～4.0mg/L, changes water every day 1 time; C, repetition b step, the zoogloea of bacterium forms after 10 days; D, change the proportioning of system's cycle of operation and water inlet nutrient solution, reactor moves 3 cycles every day, and control pH value is 7.0, and regulating aeration rate DO is 2.0mg/L, simultaneously with sewage temperature in average per 2 days 1 ℃ speed reduction reactors; When reducing to 10 ℃, water temperature changes the proportioning of the cycle of operation and water inlet nutrient solution behind e, the 10d once more, reactor moves 2 cycles every day, control pH value is 7.5～8.0, DO is 2.0mg/L, reduce sewage temperature in the reactor gradually with average per 7 days speed of 1 ℃ simultaneously, time water temperature through 5 week is reduced to 5 ℃, finishes culture of strains and domestication.
Embodiment two: present embodiment is cultivated and is tamed bacterial classification like this: a, when the sewage temperature mean value in the sanitary sewage drainage pipeline is lower than 15 ℃, get the bed mud of inspection shaft, bed mud is added in the reactor of the biochemical incubator of low temperature after filtering, precipitate, eluriating, and carries out vexed exposing to the sun under 15 ℃; B, adding fresh ight soil water and thin up to BOD concentration then is 800mg/L, ammonia nitrogen concentration is 40mg/L, phosphorus concentration is 8mg/L, be that the nutrients proportioning is BOD: N: P=100: 5: 1, regulating the pH value is 7.0, slow aeration, control DO concentration is in 2.0～4.0mg/L, change water every day 1 time, elder generation's quiescent setting is 0.9 hour when changing water, treat that newly-generated zoogloea is deposited to reactor bottom, do not precipitate in residual body of the bacterium that dies and the mineral colloid water in suspension, zoogloea and top sewage the interface is arranged comparatively clearly the time, discharge the supernatant of pond volume 50%; C, and then add fresh ight soil water and thin up to BOD concentration is 800mg/L, ammonia nitrogen concentration is 40mg/L, phosphorus concentration is 8mg/L, regulating the pH value is 7.0, slow aeration, control DO concentration is in 2.0～4.0mg/L, elder generation's quiescent setting is 0.9 hour when changing water, treat newly-generated zoogloea post precipitation, discharge the supernatant of pond volume 50%, the rest may be inferred, mud is pitchy in the starting stage reactor of cultivating, find that by sediments microscope inspection the main microbial body that exists is a free bacteria in the reactor, and move about on a small quantity flagellate and ameba arranged, mud wadding grain is little, settling ratio is near about 3%, the free bacteria and the flagellate quantity of moving about reduce after 8 days, have infusorians protozoas such as handle campanularian, mud wadding grain increases, and the zoogloea of bacterium begins to form when being suitable for 15 ℃, and sludge settling ratio increases gradually near 20%, late stage of culture, mud wadding grain is closely knit, edge clear, and settleability is good, campanularian, tired branch worm, wheel animalcule occurs in a large number, mud becomes pale brown flco after 10 days, and biological good, sludge settling ratio rises to about 30%; D, the zoogloea of bacterium forms when being suitable for 15 ℃, then change the proportioning of system's cycle of operation and nutrient solution, reactor moves 3 cycles every day, phase water inlet 1h, aeration 4h, precipitation 1h, draining 1h, idle 2h weekly, the water inlet volume is a pond volume 40%, control pH value is 7.0, and control DO is 2.0mg/L, and the proportioning mode of the nutrient solution of intaking is: in BOD: N: P=100: 5: 1 ratio adding COD=10
5The beer 50ml of mg/L, urea 0.6g, KH
2PO
40.24g, mix mutually with sanitary sewage then, be deployed into the nutrient solution of COD concentration 800mg/L, reduce sewage temperature in the reactor gradually with average per 2 days speed of 1 ℃ simultaneously, utilize COD on-line determination instrument continuous detecting water outlet COD value, find that the COD of system clearance remains in 83～90% scopes, sludge settling property descends to some extent, microscopy observation finds that the volume of activated sludge flco is big and structure is comparatively closely knit; When reducing to 10 ℃, water temperature changes the proportioning of the cycle of operation and nutrient solution behind e, the 10d (promptly cultivating 18 days) once more, reactor moves 2 cycles every day, phase water inlet 1h, aeration 8h, precipitation 2h, draining 1h, idle 1h weekly, the water inlet volume is a pond volume 40%, control pH value is 7.5～8.0, DO is 2.0mg/L, and the proportioning mode of nutrient solution is: in BOD: N: P=100: 10: 1 ratio adding COD=10
5The beer 50ml of mg/L, urea 1.2g, KH
2PO
40.24g, mix mutually with sanitary sewage then, be deployed into the nutrient solution of COD concentration 800mg/L, in order to a large amount of breeding problems of mold fungi that suppress to cause by low temperature, continue to reduce reactor temperature, 1 ℃ of system of every reduction remains on stable operation 7d under this temperature, reduce to 5 ℃ through the time water temperature in 5 week, microscopy observation finds that the activated sludge flco is loosely organized, smaller volume, the mud colour-darkening, the settling property variation, but the COD clearance still can remain between 75～80%, this shows that low temperature resistant efficient composite flora forms, and finishes spawn culture and domestication work.
Claims (7)
1, is applicable to the low temperature resistant culture of strains method that low-temperature sewage is handled, it is characterized in that it carries out according to following step: a, when the sewage temperature mean value in the sanitary sewage drainage pipeline is lower than 15 ℃, get the bed mud of inspection shaft, bed mud is added to after treatment in the reactor in the biochemical incubator of low temperature and carries out vexed exposing to the sun under 15 ℃; B, fresh ight soil water and thin up to the BOD concentration of adding are that 800mg/L, ammonia nitrogen concentration are that 40mg/L, phosphorus concentration are 8mg/L, and regulating the pH value is 7.0, slow aeration, and control DO concentration is 2.0～4.0mg/L, changes water every day 1 time; C, repetition b step, the zoogloea of bacterium when formation is suitable for 15 ℃ after 8 days; D, change the proportioning of system's cycle of operation and water inlet nutrient solution, reactor moves 3 cycles every day, and control pH value is 7.0, and regulating aeration rate DO is 2.0mg/L, simultaneously with sewage temperature in average per 2 days 1 ℃ speed reduction reactors; When reducing to 10 ℃, water temperature changes the proportioning of the cycle of operation and water inlet nutrient solution behind e, the 10d once more, reactor moves 2 cycles every day, control pH value is 7.5～8.0, DO is 2.0mg/L, reduce sewage temperature in the reactor gradually with average per 7 days speed of 1 ℃ simultaneously, time water temperature through 5 week is reduced to 5 ℃, finishes culture of strains and domestication.
2, the low temperature resistant culture of strains method that is applicable to that low-temperature sewage is handled according to claim 1, elder generation's quiescent setting is 0.9 hour when it is characterized in that changing water, discharges the supernatant of pond volume 50% then.
3, the low temperature resistant culture of strains method that is applicable to that low-temperature sewage is handled according to claim 1 is characterized in that in the d step phase water inlet 1h, aeration 4h, precipitation 1h, draining 1h, idle 2h weekly.
4, the low temperature resistant culture of strains method that is applicable to that low-temperature sewage is handled according to claim 1 is characterized in that the water inlet nutrient solution is allocated according to following step in the d step: by BOD: N: P=100: 5: 1 ratio adding COD=10
5The beer 50ml of mg/L, urea 0.6g, KH
2PO
40.24g, mix with sanitary sewage then, be deployed into the nutrient solution that COD concentration is 800mg/L.
5, the low temperature resistant culture of strains method that is applicable to that low-temperature sewage is handled according to claim 1 is characterized in that in the e step phase water inlet 1h, aeration 8h, precipitation 2h, draining 1h, idle 1h weekly.
6, the low temperature resistant culture of strains method that is applicable to that low-temperature sewage is handled according to claim 1 is characterized in that the water inlet nutrient solution is allocated according to following step in the e step: by BOD: N: P=100: 10: 1 ratio adding COD=10
5The beer 50ml of mg/L, urea 1.2g, KH
2PO
40.24g, mix with sanitary sewage then, be deployed into the nutrient solution that COD concentration is 800mg/L.
7, according to claim 3 or the 5 described low temperature resistant culture of strains methods that are applicable to that low-temperature sewage is handled, it is characterized in that into the water volume is a pond volume 40%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103803700A (en) * | 2012-11-07 | 2014-05-21 | 北京市市政四建设工程有限责任公司 | Enrichment culture method of activated sludge in sewage treatment plant in plateau alpine region |
| CN105236572A (en) * | 2015-10-27 | 2016-01-13 | 深圳德玛克环保科技有限公司 | Method for culturing phosphorus-accumulating particle sludge through low-temperature SBR technology and application |
| CN110127858A (en) * | 2019-05-14 | 2019-08-16 | 山西振钢化工有限公司 | The method of high salt tolerance strain processing organic wastewater is utilized under a kind of cryogenic conditions |
| CN115959766A (en) * | 2023-02-07 | 2023-04-14 | 广州众行环保科技有限公司 | Method for culturing activated sludge in multiple times |
-
2004
- 2004-11-12 CN CN 200410044041 patent/CN1288236C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103803700A (en) * | 2012-11-07 | 2014-05-21 | 北京市市政四建设工程有限责任公司 | Enrichment culture method of activated sludge in sewage treatment plant in plateau alpine region |
| CN103803700B (en) * | 2012-11-07 | 2015-06-03 | 北京市市政四建设工程有限责任公司 | Enrichment culture method of activated sludge in sewage treatment plant in plateau alpine region |
| CN105236572A (en) * | 2015-10-27 | 2016-01-13 | 深圳德玛克环保科技有限公司 | Method for culturing phosphorus-accumulating particle sludge through low-temperature SBR technology and application |
| CN105236572B (en) * | 2015-10-27 | 2017-11-24 | 深圳信息职业技术学院 | The method of low temperature SBR process culture polyphosphate particle sludge and application |
| CN110127858A (en) * | 2019-05-14 | 2019-08-16 | 山西振钢化工有限公司 | The method of high salt tolerance strain processing organic wastewater is utilized under a kind of cryogenic conditions |
| CN110127858B (en) * | 2019-05-14 | 2021-08-17 | 山西振钢生物科技股份有限公司 | Method for treating organic wastewater by using high-salt-resistant strain under low-temperature condition |
| CN115959766A (en) * | 2023-02-07 | 2023-04-14 | 广州众行环保科技有限公司 | Method for culturing activated sludge in multiple times |
| CN115959766B (en) * | 2023-02-07 | 2024-03-19 | 广州众行环保科技有限公司 | Fractional culture method of activated sludge |
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| CN1288236C (en) | 2006-12-06 |
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