CN107439797B - A kind of additive with livestock and poultry deodorization and its preparation method and application - Google Patents
A kind of additive with livestock and poultry deodorization and its preparation method and application Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/00—Animal feeding-stuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
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- A61L9/00—Disinfection, sterilisation or deodorisation of air
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
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Abstract
The present invention relates to a kind of additive with livestock and poultry deodorization and its preparation method and application, belong to livestock and poultry cultivation administrative skill field, the additive is made of bacillus subtilis bacterium powder, Bacillus subtilis subspecies bacterium powder, Bacillus licheniformis powder, blown-ball Azotobacter bacterium powder and wheat bran powder；The bacteria concentration of each bacterium is 2 × 10 in the additive10‑8×1010Cfu/g.The additive both can be used for the preparation of feed, again can be directly as livestock and poultry deodorant, when the feed prepared with the additive uses at the same time with the additive, before achievable feed promotes animal and bird intestines to digest, excreta is arranged while deodorization, additive works deodorization after row and bio-fertilizer conversion, or the enteron aisle that only improves for overcoming existing Chinese medicine deodorizing agent and biological deodorant carrys out deodorization, or only to the deficiency in the deodorization of excreta.
Description
Technical field
The invention belongs to livestock and poultry cultivation administrative skill field, and in particular to a kind of additive with livestock and poultry deodorization and
Its preparation method and application.
Background technology
In animal-breeding field, livestock and poultry cultivation development in China's is swift and violent, and breeding way is from free-ranging to scale, standardization cultivation
Mode changes, so that the discharged waste that animal husbandry produces compares concentration, and measures also very big, it is difficult to handle, discharged waste is
Through can not thoroughly be administered as a large amount of foul gas and sewage of important environomental pollution source, generation, become typical environment
One of public hazards.Foul gas not only pollutes air, and ecological environment is impacted, and poisonous and harmful, can cause person poultry disease
Propagation, influence surrounding resident life and livestock and poultry healthy growth.A large amount of serious sewage accumulations, also result in environment and river
Pollution, therefore, the prevention for the discharged waste that animal husbandry produces has caused the great attention of China and countries in the world government.Mesh
Before, chemical deodorizing agent, EM bacterium solutions biological deodorant and Chinese medical extract deodorant etc. have all been unable to reach a large amount of livestock and poultry excretions
The composite request prevented and control of waste, therefore, is badly in need of a kind of processing method of efficient, green a large amount of livestock and poultry discharged wastes.
The content of the invention
In view of this, it is an object of the invention to：(1) a kind of additive with livestock and poultry deodorization is provided；(2) provide
A kind of preparation method of the additive with livestock and poultry deodorization；(3) a kind of additive with livestock and poultry deodorization is provided to exist
Prepare the application in feed；(4) a kind of application of additive with livestock and poultry deodorization in deodorant is prepared is provided；(5)
There is provided one kind has livestock and poultry deodorizing method；
To reach above-mentioned purpose, the present invention provides following technical solution：
1st, a kind of additive with livestock and poultry deodorization, the additive is by bacillus subtilis bacterium powder, withered grass gemma
Bacillus withered grass subspecies bacterium powder, Bacillus licheniformis powder, blown-ball Azotobacter bacterium powder and wheat bran powder composition；It is each in the additive
The bacteria concentration of bacterium is 2 × 1010-8×1010Cfu/g；The blown-ball Azotobacter bacterium powder is made by following methods：By yucca, paddy
Bud and the extracting solution of radix glycyrrhizae add in the culture medium of the blown-ball Azotobacter fermented and cultured for being used for the bacterium, the fermented and cultured knot
Shu Hou, carries out separation of solid and liquid to zymotic fluid, is freezed after gained solid matter is stirred evenly with skimmed milk power and protective agent
After drying, crush and obtain blown-ball Azotobacter bacterium powder.
Further, the blown-ball Azotobacter bacterium powder is made by following methods：
(1) 3-5 in mass ratio:3-5:3-5 weighs yucca, rice sprout and radix glycyrrhizae, adds equivalent to 3 taste medicinal material gross weight 6-8
The water of amount decocts 2-3 times again, decocts 2-3h every time, merges decoction liquor three times and is concentrated, and gained concentrate quality is primary
0.5-1.5 times of medicine gross mass, obtains the extracting solution of yucca, rice sprout and radix glycyrrhizae；
(2) blown-ball Azotobacter is seeded in the fermentation tank of the culture medium equipped with the extracting solution containing yucca, rice sprout and radix glycyrrhizae
Fermented and cultured is carried out, after the fermented and cultured, separation of solid and liquid is carried out to zymotic fluid, by gained solid matter and skimmed milk power
After being freeze-dried after being stirred evenly with protective agent, crush and obtain blown-ball Azotobacter bacterium powder；It is described to contain extracting solution in step (1)
Culture medium by mass percentage, be made of following component：The extracting solution 4-8% of yucca, rice sprout and radix glycyrrhizae, yeast extract
0.04-0.08%, mannitol 0.8-1.5%, potassium dihydrogen phosphate 0.01-0.04%, anhydrous magnesium sulfate 0.01-0.04%, lightweight
Calcium carbonate 0.6-1.2%, ferric trichloride 0.003-0.008%, defoamer 0.06-0.15%, pH=7.0-7.5.
2nd, the preparation method of a kind of additive with livestock and poultry deodorization, includes the following steps：
(1) bacillus subtilis is seeded in the fermentation tank containing culture medium and carries out fermented and cultured, the fermented and cultured
After, separation of solid and liquid is carried out to zymotic fluid, is carried out after gained solid matter is stirred evenly with skimmed milk power and protective agent cold
It is lyophilized it is dry after, crush and obtain bacillus subtilis bacterium powder；
Preferably, the bacillus subtilis of exponential phase is seeded to the hair containing culture medium for 5-15% by volume
It is 28-30 DEG C in temperature in fermentation tank, ventilation ratio 1:0.6-1:1.5, normal pressurize is the 24- that ferments under the conditions of 0.04-0.06MPa
48h, and stir 5min, mixing speed 80-160r/min every 2h；
Preferably, the bacillus subtilis of exponential phase is seeded to the fermentation tank containing culture medium for 10% by volume
In, it is 30 DEG C in temperature, ventilation ratio 1:0.8, normal pressurize is the 30h that ferments under the conditions of 0.05MPa, and stirs 5min every 2h, is stirred
Mix 80r/min；
Preferably, the culture medium of bacillus subtilis by mass percentage, is made of following component：Peptone 2-3%,
Glucose 0.8-1.5%, sodium chloride 0.3-0.6%, dregs of beans 1-2%, manganese sulfate 0.003-0.01%, defoamer 0.08-
0.15%, pH=7.0-7.5；
Preferably, the culture medium of bacillus subtilis by mass percentage, is made of following component：Peptone 2.5%,
Glucose 1%, sodium chloride 0.5%, dregs of beans 1.5%, manganese sulfate 0.006%, defoamer 0.1%, pH=7.0；
(2) bacillus subtilis subspecies are seeded in the fermentation tank containing culture medium and carry out fermented and cultured, the fermentation
After culture, separation of solid and liquid is carried out to zymotic fluid, gained solid matter is stirred evenly with skimmed milk power and protective agent laggard
After row freeze-drying, crush and obtain bacillus subtilis subspecies bacterium powder；
Preferably, the bacillus subtilis subspecies of exponential phase are seeded to containing culture medium for 5-15% by volume
Fermentation tank in, temperature be 28-30 DEG C, ventilation ratio 1:0.6-1:1.5, normal pressurize is fermented under the conditions of being 0.04-0.06MPa
24-48h, and stir 5min, mixing speed 80-160r/min every 2h；
Preferably, the bacillus subtilis subspecies of exponential phase are seeded to the hair containing culture medium for 10% by volume
It is 30 DEG C in temperature in fermentation tank, ventilation ratio 1:0.8, normal pressurize is the 30h that ferments under the conditions of 0.05MPa, and is stirred every 2h
5min, stirs 80r/min；
Preferably, the culture medium of bacillus subtilis subspecies by mass percentage, is made of following component：Peptone 1-
2%, glucose 0.8-1.5%, wheat bran 0.8-1.5%, sodium chloride 0.3-0.6%, dregs of beans 1-2%, manganese sulfate 0.003-
0.01%, defoamer 0.08-0.15%, pH=7.0-7.5；
Preferably, the culture medium of bacillus subtilis subspecies by mass percentage, is made of following component：Peptone
1.5%, glucose 1%, wheat bran 1%, sodium chloride 0.5%, dregs of beans 1.5%, manganese sulfate 0.006%, defoamer 0.1%, pH=
7.0；
(3) bacillus licheniformis is seeded in the fermentation tank containing culture medium and carries out fermented and cultured, the fermented and cultured
After, separation of solid and liquid is carried out to zymotic fluid, is carried out after gained solid matter is stirred evenly with skimmed milk power and protective agent cold
It is lyophilized it is dry after, crush and obtain Bacillus licheniformis powder；
Preferably, the bacillus licheniformis of exponential phase is seeded to the hair containing culture medium for 5-15% by volume
It is 28-30 DEG C in temperature in fermentation tank, ventilation ratio 1:0.6-1:1.5, normal pressurize is the 24- that ferments under the conditions of 0.04-0.06MPa
48h, and stir 5min, mixing speed 80-160r/min every 2h；
Preferably, the bacillus licheniformis of exponential phase is seeded to the fermentation tank containing culture medium for 10% by volume
In, it is 30 DEG C in temperature, ventilation ratio 1:0.8, normal pressurize is the 30h that ferments under the conditions of 0.05MPa, and stirs 5min every 2h, is stirred
Mix 80r/min；
Preferably, the culture medium of bacillus licheniformis by mass percentage, is made of following component：Peptone 2-3%,
Anhydrous magnesium sulfate 0.1-0.3%, dregs of beans 1-2%, manganese sulfate 0.003-0.01%, defoamer 0.08-0.15%, pH=7.0-
7.5；
Preferably, the culture medium of bacillus licheniformis by mass percentage, is made of following component：Peptone 2.5%,
Anhydrous magnesium sulfate 0.2%, dregs of beans 1.5%, manganese sulfate 0.006%, defoamer 0.1%, pH=7.0；
(4) blown-ball Azotobacter is seeded in the fermentation tank of the culture medium equipped with the extracting solution containing yucca, rice sprout and radix glycyrrhizae
Fermented and cultured is carried out, after the fermented and cultured, separation of solid and liquid is carried out to zymotic fluid, by gained solid matter and skimmed milk power
After being freeze-dried after being stirred evenly with protective agent, crush and obtain blown-ball Azotobacter bacterium powder；
Preferably, the blown-ball Azotobacter of exponential phase is seeded to equipped with containing yucca, rice sprout for 5-15% by volume
It is 28-30 DEG C in temperature in the fermentation tank of the culture medium of the extracting solution of radix glycyrrhizae, ventilation ratio 1:0.6-1:1.5, normal pressurize is
Ferment 30-52h under the conditions of 0.04-0.06MPa, stirs 5min every 2h, stirs 80r/min；
Preferably, by the blown-ball Azotobacter of exponential phase by volume for 10% be seeded to equipped with containing yucca, rice sprout and
It is 30 DEG C in temperature in the fermentation tank of the culture medium of the extracting solution of radix glycyrrhizae, ventilation ratio 1:1, under the conditions of normal pressurize is 0.05MPa
Ferment 48h, stirs 5min every 2h, stirs 80-160r/min；
Preferably, 3-5 in mass ratio:3-5:3-5 weighs yucca, rice sprout and radix glycyrrhizae, adds equivalent to 3 taste medicinal material gross weights
The 6-8 times of water measured decocts 2-3 times, decocts 2-3h every time, merges decoction liquor three times and is concentrated, and gained concentrate quality is original
0.5-1.5 times of crude drug gross mass, obtains the extracting solution of yucca, rice sprout and radix glycyrrhizae, obtains the extraction of yucca, rice sprout and radix glycyrrhizae
Liquid；
Preferably, in mass ratio 4:3:3 weigh yucca, rice sprout and radix glycyrrhizae, add for the first time equivalent to 3 taste medicinal material gross weights
8 times of water, decocts 3h, filtering, collects decoction liquor；The dregs of a decoction add the water equivalent to 36 times of taste medicinal material gross weight, decoct 2h, mistake
Filter, collects decoction liquor；The dregs of a decoction add the water equivalent to 35 times of taste medicinal material gross weight, decoct 1h, filtering, collects decoction liquor；Close
And decoction liquor and concentrated three times, gained concentrate quality is 1.0 times of crude drug in whole gross mass, obtains yucca, rice sprout and sweet
The extracting solution of grass；
Preferably, the culture medium of blown-ball Azotobacter by mass percentage, is made of following component：Yucca, rice sprout and sweet
The extracting solution 4-8%, yeast extract 0.04-0.08%, mannitol 0.8-1.5% of grass, potassium dihydrogen phosphate 0.01-0.04%, nothing
Water magnesium sulfate 0.01-0.04%, precipitated calcium carbonate 0.6-1.2%, ferric trichloride 0.003-0.008%, defoamer 0.06-
0.15%, pH=7.0-7.5；
Preferably, the culture medium of blown-ball Azotobacter by mass percentage, is made of following component：Yucca, rice sprout and sweet
The extracting solution 5% of grass, yeast extract 0.05%, mannitol 1.0%, potassium dihydrogen phosphate 0.03%, anhydrous magnesium sulfate 0.02%, gently
Matter calcium carbonate 0.8%, ferric trichloride 0.005%, defoamer 0.1%, pH=7.2；
(5) by each bacterium powder in step (1)-(4) and wheat bran powder after mixing, adding with livestock and poultry deodorization is made
Add agent.
3rd, application of a kind of additive with livestock and poultry deodorization in feed is prepared.
Further, additive amount of the additive in feed is 0.1-0.2wt%.
4th, application of a kind of additive with livestock and poultry deodorization in deodorant is prepared.
5th, a kind of method for handling a large amount of livestock and poultry discharged wastes, the method are specially：, will be described during its feeding
A kind of additive with livestock and poultry deodorization is mixed by the dosage of 1-2g/kg with bedding and padding obtains mixture, in the mixture
Moisture is 40-50wt%, while feeds the feed containing a kind of additive with livestock and poultry deodorization.
The beneficial effects of the present invention are：The present invention provides a kind of additive with livestock and poultry deodorization and its preparation
Methods and applications, the additive not only can be used for the preparation of feed, but also can be directly as livestock and poultry deodorant, when with the addition
Feed prepared by agent is with the additive at the same time in use, deodorization before the digestion of feed promotion animal and bird intestines, excreta row can be realized
Meanwhile additive works deodorization after row and bio-fertilizer conversion, overcomes existing Chinese medicine deodorizing agent and biological deodorant
Only improve enteron aisle and carry out deodorization, or only to the deficiency in the deodorization of excreta.
In the additive in the preparation process of blown-ball Azotobacter bacterium powder, trained using the extracting solution of yucca, rice sprout and radix glycyrrhizae as it
Support base component one cultivates it, can effectively strengthen the nitrogen fixing capacity of the bacterium, the adaptability in animal and bird intestines and steady
Qualitative genetic breeding ability, wherein, the material such as the yucconin from yucca, free saponin, carbohydrate complexes exists in extracting solution
Blown-ball Azotobacter nitrogen fixing capacity can be improved in fermentation process, reduces the percentage that enteron aisle nitrogen substance is converted into ammonia, methylamine etc.
Than；Protein from rice sprout, fat oil, starch, amylase, maltose in extracting solution etc. can improve the numerous of blown-ball Azotobacter
Reproductive growth ability and the ability that its bioactivity is kept in enteron aisle；The glycyrrhizic acid from radix glycyrrhizae in extracting solution, it is liquiritin, sweet
The materials such as careless flavonoids can prevent the nongenetic of bacterium from making a variation in blown-ball Azotobacter fermentation, and it is numerous to make it have stability heredity
Ability is grown, and the toxic side effect of blown-ball Azotobacter metabolite can be reduced.Correspondingly, extracting solution ferments through blown-ball Azotobacter
Afterwards, the material such as the tannin in extracting solution, protein, amino acid, polysaccharide can be converted into beneficial to the active organic of livestock and poultry absorption
Thing, wherein, after the fermentation of materials such as yucconin, free saponin, liquiritin, flavonoids of Glycyrrhiza, urease inhibitor, extraction can be made
The adsorption capacity and increased activity of active ingredient in liquid, and the acidic materials energy that blown-ball Azotobacter produces during the fermentation
Enough improve the acid-base balance of intestines and stomach, higher activity can be kept in intestines and stomach, promotion digestion is played, it is dirty to reduce ammonia nitrogen
Contaminate the effect of the generation of thing.Finally, to carrying out freeze-drying process after final zymotic fluid separation of solid and liquid, make in obtained bacterium powder both
Containing considerable amount of thalline, and containing the active ingredient in the extracting solution after blown-ball Azotobacter ferments, it ensure that blown-ball Azotobacter
The activity of active ingredient in bacterium work amount and extracting solution.
Embodiment
The preferred embodiment of the present invention will be described in detail below.
Bacillus subtilis (Bacillus subtilis ACCC11025) in the strain used in the present invention, in being purchased from
Agricultural Microbiological Culture Collection administrative center of state (ACCC)；Bacillus licheniformis (Bacillus licheniformis
ACCC06149), it is purchased from Chinese agriculture Microbiological Culture Collection administrative center (ACCC)；Blown-ball Azotobacter (Azotobacter
Chroococcum CICC 21685), it is purchased from Chinese industrial Microbiological Culture Collection administrative center (CICC)；Bacillus subtilis
Withered grass subspecies (Bacillus subtilis subsp.Subtilis CICC 10366), are purchased from Chinese industrial microorganism fungus kind
Preservation administrative center (CICC).
Embodiment 1
1st, a kind of additive with livestock and poultry deodorization is prepared
(1) prepared by bacillus subtilis bacterium powder
It is 2m that the bacillus subtilis of exponential phase is seeded to the volume containing culture medium for 10% by volume3Hair
It is 30 DEG C in temperature in fermentation tank, ventilation ratio 1:0.8, temperature is reduced to 26 DEG C after fermentation 30h under the conditions of normal pressurize is 0.05MPa,
Period every 2h stir 5min, stir 80r/min, wherein, the culture medium of bacillus subtilis by mass percentage, by as follows
Component forms：Peptone 2.5%, glucose 1%, sodium chloride 0.5%, dregs of beans 1.5%, manganese sulfate 0.006%, defoamer
0.1%, pH=7.0.After fermented and cultured, separation of solid and liquid is carried out to zymotic fluid, by gained solid matter and skimmed milk power and
After protective agent is freeze-dried after stirring evenly under the conditions of -35 DEG C, crushes and obtain bacillus subtilis bacterium powder, in the bacterium powder
Bacterial content is 2 × 1011Cfu/g。
(2) prepared by bacillus subtilis subspecies bacterium powder
It is 2m that the bacillus subtilis subspecies of exponential phase are seeded to the volume containing culture medium for 10% by volume3
Fermentation tank in, temperature be 30 DEG C, ventilation ratio 1:0.8, under the conditions of normal pressurize is 0.05MPa temperature is reduced after fermentation 30h extremely
26 DEG C, 5min during which is stirred every 2h, stirs 80r/min, wherein, the culture medium of bacillus subtilis subspecies is by mass percentage
Meter, is made of following component：Peptone 1.5%, glucose 1%, wheat bran 1%, sodium chloride 0.5%, dregs of beans 1.5%, manganese sulfate
0.006%, defoamer 0.1%, pH=7.0.After fermented and cultured, separation of solid and liquid is carried out to zymotic fluid, by gained solid
After matter is freeze-dried after being stirred evenly with skimmed milk power and protective agent under the conditions of -35 DEG C, crushes and obtain bacillus subtilis
Bacterium subspecies bacterium powder, bacterial content is 2 × 10 in the bacterium powder11Cfu/g。
(3) prepared by Bacillus licheniformis powder
It is 2m that the bacillus licheniformis of exponential phase is seeded to the volume containing culture medium for 10% by volume3Hair
It is 30 DEG C in temperature in fermentation tank, ventilation ratio 1:0.8, temperature is reduced to 26 DEG C after fermentation 30h under the conditions of normal pressurize is 0.05MPa,
Period every 2h stir 5min, stir 80r/min, wherein, the culture medium of bacillus licheniformis by mass percentage, by as follows
Component forms：Peptone 2.5%, anhydrous magnesium sulfate 0.2%, dregs of beans 1.5%, manganese sulfate 0.006%, defoamer 0.1%, pH=
7.0.After fermented and cultured, separation of solid and liquid is carried out to zymotic fluid, gained solid matter and skimmed milk power and protective agent are stirred
After being freeze-dried after uniformly under the conditions of -35 DEG C, crush and obtain Bacillus licheniformis powder, bacterial content is in the bacterium powder
2×1011Cfu/g。
(4) prepared by blown-ball Azotobacter bacterium powder
In mass ratio 4:3:3 weigh yucca, rice sprout and radix glycyrrhizae, add for the first time equivalent to 38 times of taste medicinal material gross weights
Water, decocts 3h, filtering, collects decoction liquor；The dregs of a decoction add the water equivalent to 36 times of taste medicinal material gross weight, decoct 2h, filter, and receive
Collect decoction liquor；The dregs of a decoction add the water equivalent to 35 times of taste medicinal material gross weight, decoct 1h, filtering, collects decoction liquor；Merge three times
Decoction liquor is simultaneously concentrated, and gained concentrate quality obtains yucca, rice sprout and radix glycyrrhizae equivalent to 1.0 times of crude drug in whole gross mass
Extracting solution；
It is 2m that the blown-ball Azotobacter of exponential phase is seeded to the volume containing culture medium for 10% by volume3Fermentation
It is 30 DEG C in temperature in tank, ventilation ratio 1:1, temperature is reduced to 26 DEG C after fermentation 48h under the conditions of normal pressurize is 0.05MPa, during which
Every 2h stir 5min, stir 80r/min, wherein, the culture medium of blown-ball Azotobacter by mass percentage, by following component group
Into：The extracting solution 5% (per L equivalent to crude drug in whole 1kg) of yucca, rice sprout and radix glycyrrhizae, yeast extract 0.05%, mannitol 1.0%,
Potassium dihydrogen phosphate 0.03%, anhydrous magnesium sulfate 0.02%, precipitated calcium carbonate 0.8%, ferric trichloride 0.005%, defoamer
0.1%, pH=7.2.After fermented and cultured, separation of solid and liquid is carried out to zymotic fluid, by gained solid matter and skimmed milk power and
After protective agent is freeze-dried after stirring evenly under the conditions of -35 DEG C, crushes and obtain blown-ball Azotobacter bacterium powder, bacterium in the bacterium powder
Content is 2 × 1011Cfu/g。
(5) by bacillus subtilis bacterium powder produced above, bacillus subtilis subspecies bacterium powder, Bacillus licheniformis
Powder, blown-ball Azotobacter bacterium powder and wheat bran powder in mass ratio 1：1：1：1：6 after mixing, is made with livestock and poultry deodorization
Additive, then the bacteria concentration of each bacterium is 2 × 10 in the additive10Cfu/g。
2nd, prepare containing the feed with livestock and poultry deodorization additive
By the additive and normal diet in mass ratio 1 with livestock and poultry deodorization of above-mentioned preparation：500 are mixed,
Mixed feed is made.
Embodiment 2
Additive with livestock and poultry deodorization and the answering in livestock and poultry deodorization with the mixed feed of additive preparation
With
1st, the application in swine rearing
Choose 4, pig farm behaviour area and excretion distinguishes colony house, each colony house raises life of 10 weight in 30kg-40kg
Long fattening pig, is divided into as follows labeled as control group 1#, control group 2# and test group 3#, test group 4#, each group condition setting：
Control group 1#：Discharge area is rinsed with the water of 30L daily and presses 1.2-1.5kg/ to scratch pool, while with common pannage
Store pig in the scale of feeding breeding pen in head/day.
Control group 2#：Every 3 days to discharge area spreading 100kg with bedding and padding made of chaff powder, sawdust, straw powder etc., will pad
Moisture in material is controlled in 40-50%, while with common pannage by raw in the scale of feeding breeding pen in 1.2-1.5kg/ heads/day
Long fattening pig.
Test group 3#：Every 3 days to discharge area spreading 100kg with bedding and padding made of chaff powder, sawdust, straw powder etc., but should
The additive with livestock and poultry deodorization prepared in 0.2kg embodiments 1 is mixed with bedding and padding, the moisture control in bedding and padding is existed
40-50%, while with common pannage by store pig in the scale of feeding breeding pen in 1.2-1.5kg/ heads/day.
Test group 4#：Every 3 days to discharge area spreading 100kg with bedding and padding made of chaff powder, sawdust, straw powder etc., but should
The additive with livestock and poultry deodorization prepared in 0.2kg embodiments 1 is mixed with bedding and padding, the moisture control in bedding and padding is existed
40-50%, while the mixed feed to be prepared in embodiment 1 is by raw in the scale of feeding breeding pen in 1.2-1.5kg/ heads/day
Long fattening pig, the mixed feed is the additive with livestock and poultry deodorization and common pannage in mass ratio 1 at this time：500
Mix.
Above-mentioned four groups of feeding times 30 days, every 7 days respectively to the instant excrement, urine and the pad of discharge area of four groups of test pigs
It is separately sampled to expect that mixture carries out, testing result is shown in Table 1：
The testing result of the instant excrement of 1 four groups of test pigs of table, urine and the bedding and padding of discharge area mixture
As shown in Table 1, in control group 2# the deodorization leading indicator of excreta mixture compared with control group 1# almost without
What change；COD, BOD in the deodorization leading indicator of excreta mixture in test group 3#5, NH3-N, TP and H2S and control group
1# is compared and be have dropped 27.48%, 21.37%, 21.30%, 18.96%, 27.16% respectively, and total nitrogen index TN rises
19.69%；COD, BOD in the deodorization leading indicator of pig manure in test group 4#5, NH3-N, TP and H2S distinguishes compared with control group 1#
29.91%, 31.31%, 39.23%, 29.91%, 31.22% is have dropped, and total nitrogen index TN rises 22.14%；Experiment
COD, BOD in the deodorization leading indicator that pig urinates in group 4#5, NH3-N, TP and H2S have dropped respectively compared with control group 1#
34.43%th, 28.13%, 36.96%, 36.00%, 35.14%, and total nitrogen index TN rises 23.12%；In test group 4#
COD, BOD in the deodorization leading indicator of excreta mixture5, NH3-N, TP and H2S have dropped respectively compared with control group 1#
33.68%th, 32.84%, 34.35%, 31.28%, 24.57%, and total nitrogen index TN rises 28.54%.Therefore, by right
Than understanding, in test group 3#, by using the additive in the present invention, fermented and adsorbed using microorganism species in the additive
Effect, can substantially reach deodorizing effect, and by the nitrogen fixation of wherein blown-ball Azotobacter, significantly improve total nitrogen index,
Particularly, in test group 4#, while during using the additive in the present invention with feed containing the additive, deodorizing effect and solid
Nitrogen effect is more notable, otherwise the enteron aisle that only improves for overcoming existing Chinese medicine deodorizing agent and biological deodorant carrys out deodorization, or only
To the deficiency in the deodorization of excreta.
2nd, the application in fowl raising
1 colony house of chicken house is chosen, amounts to 400 squares, every 100 squares turn to a region, are divided into 4 regions, Mei Gequ
120 age in days laying hens of about 6500, domain, every chicken average weight in 1.2-1.5kg, be respectively labeled as control group 1#, control group 2#,
Test group 3# and test group 4#, each group condition setting are as follows：
Control group 1#：Any measure is not taken to the region, is raised with common chicken feed by 90-120g/ pcs/day of scale of feeding
Support laying hen in the region.
Control group 2#：Every 3 days to ground excreta spreading 200kg with bedding and padding made of chaff powder, sawdust, straw powder etc.,
Moisture in bedding and padding is controlled in 40-50%, while with common chicken feed and raises the region by 90-120g/ pcs/day of scale of feeding
Middle laying hen.
Test group 3#：Every 3 days to ground excreta spreading 200kg with bedding and padding made of chaff powder, sawdust, straw powder etc.,
But the additive with livestock and poultry deodorization prepared in 0.2kg embodiments 1 is mixed with the bedding and padding, by the moisture control in bedding and padding
System raises laying hen in the region in 40-50%, while with common chicken feed by 90-120g/ pcs/day of scale of feeding.
Test group 4#：Every 3 days to ground excreta spreading 200kg with bedding and padding made of chaff powder, sawdust, straw powder etc.,
But the additive with livestock and poultry deodorization prepared in 0.2kg embodiments 1 is mixed with the bedding and padding, by the moisture control in bedding and padding
System is in 40-50%, while the mixed feed to be prepared in embodiment 1 raises the region by 90-120g/ pcs/day of scale of feeding
Middle laying hen, the mixed feed is the additive with livestock and poultry deodorization and common chicken feed in mass ratio 1 at this time：500 is mixed
Conjunction forms.
Above-mentioned four groups of feeding times 30 days, every 7 days instant excrement to four groups of test chickens and the bedding and padding of discharge area respectively
Mixture progress is separately sampled, and testing result is shown in Table 2.
As shown in Table 2, in control group 2# the deodorization leading indicator of excreta mixture compared with control group 1# almost without
What change；COD, BOD in the deodorization leading indicator of excreta mixture in test group 3#5, NH3-N, TP and H2S and control group
1# is compared and be have dropped 18.07%, 19.15%, 21.53%, 16.09%, 13.17% respectively, and total nitrogen index TN rises
13.17%；COD, BOD in the deodorization leading indicator of chicken manure in test group 4#5, NH3-N, TP and H2S distinguishes compared with control group 1#
28.91%, 26.82%, 28.63%, 26.70%, 26.86% is have dropped, and total nitrogen index TN rises 21.33%；Experiment
Group 4# in excreta mixture deodorization leading indicator in COD, BOD5, NH3-N, TP and H2S declines respectively compared with control group 1#
28.47%, 30.85%, 29.67%, 28.76%, 26.86%, and total nitrogen index TN rises 22.36%.Therefore, pass through
Contrast is understood, in test group 3#, by using the additive in the present invention, is fermented and inhaled using microorganism species in the additive
Attached effect, can substantially reach deodorizing effect, and by the nitrogen fixation of wherein blown-ball Azotobacter, total nitrogen index is substantially carried
Height, particularly, in test group 4#, while during using the additive in the present invention with feed containing the additive, deodorizing effect
, or the enteron aisle that only improves for overcoming existing Chinese medicine deodorizing agent and biological deodorant carrys out deodorization and nitrogen fixation effect is more notable
Only to the deficiency in the deodorization of excreta.
The testing result of the instant excrement of 2 four groups of test chickens of table and the bedding and padding mixture of discharge area
Finally illustrate, preferred embodiment above is merely illustrative of the technical solution of the present invention and unrestricted, although logical
Cross above preferred embodiment the present invention is described in detail, however, those skilled in the art should understand that, can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (7)
1. a kind of additive with livestock and poultry deodorization, it is characterised in that the additive is by bacillus subtilis bacterium powder, withered
Careless subtilis subspecies bacterium powder, Bacillus licheniformis powder, blown-ball Azotobacter bacterium powder and wheat bran powder composition；The addition
The bacteria concentration of each bacterium is 2 × 10 in agent10 -8×1010Cfu/g；The blown-ball Azotobacter bacterium powder is made by following methods：Will
The extracting solution of yucca, rice sprout and radix glycyrrhizae adds in the culture medium of the blown-ball Azotobacter fermented and cultured for being used for the bacterium, the hair
After ferment culture, separation of solid and liquid is carried out to zymotic fluid, after gained solid matter and skimmed milk power and protective agent are stirred evenly
After being freeze-dried, crush and obtain blown-ball Azotobacter bacterium powder；The preparation method of the extracting solution of the yucca, rice sprout and radix glycyrrhizae is such as
Under：3-5 in mass ratio:3-5:3-5 weighs yucca, rice sprout and radix glycyrrhizae, adds equivalent to 3 6-8 times of water measured of taste medicinal material gross weight
Decoct 2-3 times, decoct 2-3h every time, merge decoction liquor three times and concentrated, gained concentrate quality is crude drug in whole gross mass
0.5-1.5 times, obtain the extracting solution of yucca, rice sprout and radix glycyrrhizae.
A kind of 2. additive with livestock and poultry deodorization as claimed in claim 1, it is characterised in that the blown-ball Azotobacter
Bacterium powder is made by following methods：
（1）3-5 in mass ratio:3-5:3-5 weighs yucca, rice sprout and radix glycyrrhizae, adds and is measured equivalent to 3 6-8 times of taste medicinal material gross weights
Water decoct 2-3 time, decoct 2-3h every time, merge decoction liquor three times and concentrated, gained concentrate quality is that crude drug in whole is total
0.5-1.5 times of quality, obtains the extracting solution of yucca, rice sprout and radix glycyrrhizae；
（2）Blown-ball Azotobacter is seeded in the fermentation tank of the culture medium equipped with the extracting solution containing yucca, rice sprout and radix glycyrrhizae and is carried out
Fermented and cultured, after the fermented and cultured, carries out separation of solid and liquid, by gained solid matter and skimmed milk power and guarantor to zymotic fluid
After shield agent is freeze-dried after stirring evenly, crushes and obtain blown-ball Azotobacter bacterium powder；It is described to contain step（1）The training of middle extracting solution
Foster base by mass percentage, is made of following component：The extracting solution 4-8% of yucca, rice sprout and radix glycyrrhizae, yeast extract 0.04-
0.08%, mannitol 0.8-1.5%, potassium dihydrogen phosphate 0.01-0.04%, anhydrous magnesium sulfate 0.01-0.04%, precipitated calcium carbonate 0.6-
1.2%, ferric trichloride 0.003-0.008%, defoamer 0.06-0.15%, pH=7.0-7.5.
A kind of 3. preparation method of additive with livestock and poultry deodorization described in claim 1 or 2, it is characterised in that bag
Include following steps：
（1）Bacillus subtilis is seeded in the fermentation tank containing culture medium and carries out fermented and cultured, the fermented and cultured terminates
Afterwards, separation of solid and liquid is carried out to zymotic fluid, it is dry that freezing is carried out after gained solid matter and skimmed milk power and protective agent are stirred evenly
After dry, crush and obtain bacillus subtilis bacterium powder；
（2）Bacillus subtilis subspecies are seeded in the fermentation tank containing culture medium and carry out fermented and cultured, the fermented and cultured
After, separation of solid and liquid is carried out to zymotic fluid, is carried out after gained solid matter is stirred evenly with skimmed milk power and protective agent cold
It is lyophilized it is dry after, crush and obtain bacillus subtilis subspecies bacterium powder；
（3）Bacillus licheniformis is seeded in the fermentation tank containing culture medium and carries out fermented and cultured, the fermented and cultured terminates
Afterwards, separation of solid and liquid is carried out to zymotic fluid, it is dry that freezing is carried out after gained solid matter and skimmed milk power and protective agent are stirred evenly
After dry, crush and obtain Bacillus licheniformis powder；
（4）Blown-ball Azotobacter is seeded in the fermentation tank of the culture medium equipped with the extracting solution containing yucca, rice sprout and radix glycyrrhizae and is carried out
Fermented and cultured, after the fermented and cultured, carries out separation of solid and liquid, by gained solid matter and skimmed milk power and guarantor to zymotic fluid
After shield agent is freeze-dried after stirring evenly, crushes and obtain blown-ball Azotobacter bacterium powder；
（5）By step（1）-（4）In each bacterium powder and wheat bran powder after mixing, the addition with livestock and poultry deodorization is made
Agent.
A kind of 4. application of additive with livestock and poultry deodorization in feed is prepared described in claim 1 or 2.
5. application as claimed in claim 4, it is characterised in that additive amount of the additive in feed is 0.1-
0.2wt%。
A kind of 6. application of additive with livestock and poultry deodorization in deodorant is prepared described in claim 1 or 2.
A kind of 7. method for handling a large amount of livestock and poultry discharged wastes, it is characterised in that the method is specially：During its feeding,
A kind of additive with livestock and poultry deodorization described in claim 1 or 2 is mixed into acquisition with bedding and padding by the dosage of 1-2g/kg
Mixture, moisture is 40-50wt% in the mixture, while feeds and have poultry containing one kind described in claim 1 or 2
The feed of the additive of fowl deodorization.
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| CN108101326A (en) * | 2017-12-26 | 2018-06-01 | 安徽仁维环保工程科技有限公司 | A kind of microbial deodorant |
| CN108902493A (en) * | 2018-06-26 | 2018-11-30 | 北京鹤来科技有限公司 | A kind of herd boar concentrated feed |
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