CN113973999B

CN113973999B - Fermented compound traditional Chinese medicine additive for improving water content and PSY of sows and sheep and preparation method thereof

Info

Publication number: CN113973999B
Application number: CN202111256952.6A
Authority: CN
Inventors: 何余湧; 陆伟; 曹远东; 吴华东; 练小华; 郭起山; 谢益根; 邹莞杰
Original assignee: Jiangxi Agricultural University
Current assignee: Jiangxi Agricultural University
Priority date: 2021-10-27
Filing date: 2021-10-27
Publication date: 2023-07-07
Anticipated expiration: 2041-10-27
Also published as: CN113973999A

Abstract

The invention discloses a fermented compound traditional Chinese medicine additive for improving the water content of sows and the number of weaned Pigs (PSY) and a preparation method thereof. The fermented compound traditional Chinese medicine additive reasonably selects various traditional Chinese medicine components according to the medicinal components and pharmacological characteristics of the traditional Chinese medicine, has various medicinal components with the effects of resisting oxidation, resisting bacteria, diminishing inflammation, protecting cardiovascular diseases, accelerating blood circulation and increasing vascular permeability, moderately increases the sheep water quantity of pregnant sows under the synergistic effect of the medicinal components, ensures that the birth canal is more lubricated, shortens the sow birth process, can improve the milk quality of the sows, improves the vitality and survival rate of piglets, and achieves the aim of improving the head number of weaned piglets in the litter of the sows.

Description

Fermented compound traditional Chinese medicine additive for improving water content and PSY of sows and sheep and preparation method thereof

Technical Field

The invention belongs to the technical field of feed additives, and in particular relates to a fermented compound traditional Chinese medicine additive for improving the water content and PSY of sows and sheep and a preparation method thereof.

Background

Sow labor refers to the time it takes a sow to expel all the fetus from the body to the outside of the body at the time of delivery. In actual production, the time required for normal delivery of the sow (labor) was on average 4 hours (calculated as delivery of 12 piglets), and the average delivery interval between the two piglets was 20 minutes. However, in our investigation, it was found that the phenomenon of too long a sow in the pig farm (especially in summer) is very common, with a labor time of up to 5-8 hours, and even a fraction of sows up to 24-48 hours.

A series of hazards can be generated when the sow is in too long a labor course: firstly, the harm to sows is that the sows are extremely easy to generate delivery syndrome (extremely tired, severe pain and metabolic disturbance) after delivery, the delivery syndrome is manifested by poor postpartum mental state caused by excessive physical energy consumption, birth canal bleeding and vaginal household edema, and even death caused by vaginal and uterine prolapse or uterine rupture and massive hemorrhage; the sow has the advantages of no postpartum fever, difficult appetite recovery, lochia inexhaustibility, postpartum infection and the like, has high incidence rate of endometritis, greatly prolongs the recovery time of the normal function of uterus, and does not have oestrus for a long time after weaning; affecting the recovery of the normal appetite of the sow, resulting in the milk deficiency of the sow. Secondly, the harm to piglets is that the sow has too long a labor, and the longer the fetus is extruded in the uterus and the birth canal. Each time of uterine contraction and knoop can cause temporary interruption of blood supply of the fetus, long-time uterine contraction and knoop can cause persistent hypoxia of the fetus, and the intrauterine embarrassment and asphyxia of the fetus are caused, so that the weaned pigs and the shaggy pigs are obviously increased, death occurs in the delivery process, and the death rate is increased. Weak piglets tend to have poor vitality after birth, enough colostrum is not eaten, enough maternal protection can not be obtained, the conditions of diarrhea and yellow-white dysentery in lactation are increased, and the survival rate is low.

There are many reasons for the too long labor of sows, of which hypoamniotic fluid is one of the most important reasons. Amniotic fluid refers to a fluid filled around a fetus during gestation and surrounded by amniotic membrane (see FIG. 1), which consists essentially of placental permeate, fetal alveolar fluid and fetal urine [ Setnikar, et al, the fetalus lung, a source of amniotic fluid, proc Soc Exp Biol Med 1959, 101:842-845; brace, et al Regulation of amniotic fluid volume: insights derived from amniotic fluid volume function cultures.Am J Physiol Regul Integr Comp Physiol.2018,315:R777-R789. When amniotic fluid is excessive, normal water balance can be maintained by swallowing and intra-amniotic absorption of the fetus [ bell, et al regulation of amniotic fluid volume Plamenta, 2008, 28:824-832; brace, et al Regulation of amniotic fluid volume: insights derived from amniotic fluid volume function cultures.Am J Physiol Regul Integr Comp Physiol.2018,315:R777-R789. Normal amniotic fluid volume in the amniotic cavity may provide protection for fetal movement, growth and health [ Dubil and mangen. Ambaritic fluid as a vital sign for fetal wellbearing. Australias J Ultrasound med.2013,16 (2): 62-70 ]. Fetal swallowing of amniotic fluid helps reduce the incidence of necrotic enteritis in newborn piglets [ Good, et al, ampholytic fluid attenuates experimental murine and piglet necrotizing enterocolitis via reduced TLR signaling, gastroenterology,2013,144 (5): S314-S314 ] ], whereas fetal swallowing of amniotic fluid is almost zero when the amount of amniotic fluid is well below normal [ brain, et al, fatal swallowing as a protective mechanism against oligohydramnios and polyhydramnios in late gestation light, reprod sci.2013,20 (3): 326-330 ] ], and therefore, maintaining normal amniotic fluid is very important for fetal health and development. The lack of amniotic fluid can increase the mortality of fetuses and newborns, and can also obstruct the growth of fetuses, so that the fetuses are underdeveloped, pulmonary diseases are very easy to occur after the fetuses are born, and even the neonates die. Insufficient amniotic fluid during delivery may make the fetus difficult to break the amniotic membrane, prolonging the delivery time [ thibult, et al, neonaal pulmonary hypoplasia with premature rupture of fetal membranes and oligohydrammos.J Pediatr.1985, 107:273-277; kitterman, et al, effects of oligohydramnios on lung growth and maturation in the fetal at the rate of Am J Physiol Lung Cell Mol physiol 2002,282:L431-L439 ], in addition, because the amniotic fluid contains a large amount of organic matters, when the amniotic fluid is too little, the amniotic fluid in the amniotic cavity becomes viscous, the too little amniotic fluid and the relatively high viscosity thereof cause the increase of resistance of the birth canal, the piglets cannot be rapidly discharged from the birth canal, the hypoxia in the birth canal is caused, and the hypoxia can cause the serious reduction of the vigor of the primary piglets or the choking death in the birth canal of a fetus, so that the number of the produced and alive piglets of the sow is reduced. Studies have shown that the amniotic fluid in each amniotic cavity in a sow slowly increases from an average of 1.7 ml at 30 days of pregnancy to 201.3 ml at 85 days, then decreases to 107.4 ml at 105 days, and then approximately 26.3 ml of amniotic fluid at 112 days [ Goldstein, et al, hybridization of changes in volume, osmolarity and electrolyte composition of porcine fetal fluids during gel station. Biol reprod.1980,22 (5): 1168-1180 ].

In the middle and late gestation period, amniotic fluid volume mainly depends on urine excretion and alveolar fluid secretion of a fetus, proline in fetal urine is the most important amino acid required for fetal lung maturation, and insufficient proline content is likely to cause fetal lung hypoplasia when amniotic fluid is too low [ Harman. Amniotic fluids abominable chemicals. Semin Perinant.2008, 32:288-294; wu, et al Pulmonary Hypoplasia Induced by Oligohydramnios: findings from Animal Models and a Population-Based student, pedia Neonatl.2017, 58:3-7; weiner, et al, amniotic fluid volume at presentation with early preterm prelabor rupture of membranes and association with severe neonatal respiratory mobility, ultrasonic bond Obstet Gynecol.2019,54:767-773 ] is prone to respiratory disease after birth and even causes death of the neonate due to respiratory abnormalities [ Tanahashi, et al, polyhydroamnios in Lrp4 knockout mice with bilateral kidney agenesis: defects in the pathways of amniotic fluid clearance.Sci Rep.2016,6:20241 ]. In the middle and late gestation, the fetus can ingest nutrients, active factors and precursors of synthetic antibacterial substances by swallowing amniotic fluid, thereby promoting its own growth and intestinal maturation [ Li, et al, permeation in the biochemical composition of fetal fluids are apparent in surviving bovine somatic cell nuclear transfer pregnancies in the first half of station. Biol reprod.2005,73 (1): 139-148.; buddington, et al, prenatal gastrointestinal development in the pig and responses after preterm birth.J Anim Sci.2012; 90:290-298; wu, et al biological mechanisms for nutritional regulation of maternal health and fetal development, paediatr Perinat epidemic 2012;26:4-26.]. Amniotic fluid plays a more remarkable role in the last stage of pregnancy as a nutritional pathway of the fetus, and if this pathway is blocked, it leads to retarded intrauterine growth of the fetus (intrauterine growth retardation, IUGR) [ Wang, et al, tempororal proteomic analysis reveals defects in small-intestinal development of porcine fetuses with intrauterine growth, restriction.j Nutr biochem.2014,25 (7): 785-795 ].

In a large-scale pig farm, in order to pursue a large primary weight, the sow is subjected to fetal tapping in the later period of pregnancy, namely, the feeding amount and the nutrition concentration of the pregnant sow are increased, so that the nutrition substances such as protein and the like fed by the pregnant sow far exceed the normal requirement. In addition, the sow feed often contains mycotoxin, and long-term ingestion of the feed can cause accumulation of mycotoxin in a body, so that cardiovascular and fetal lung and urinary organs of the sow are damaged, and the blood supply of the sow to the fetus is insufficient, and the fetal alveolar fluid and urine are formed insufficiently.

How to increase the amount of amniotic fluid in the antenatal sow is a urgent problem to be solved in the current pig farm production, because sufficient amniotic fluid can enable the sow birth canal to be smooth during delivery, the formed water sac has the functions of softening and expanding the cervix and the birth canal of the parent, so that the fetus can be conveniently and smoothly produced, and the harm of dystocia to the sow and the piglet can be greatly reduced; in addition, sufficient amniotic fluid can inhibit bacteria to reduce birth canal infection.

Uterine-vaginal-mastitis can reduce the annual average weaned pig count (PSY) of a Sow, because long-term uterine vaginitis of the Sow can easily cause weak implantation of fertilized eggs, so that symptoms such as increased embryonic death, blocked intrauterine development of a fetus or Sow oestrus return occur, and the like, while mastitis can easily cause the milk yield and quality of the lactating Sow to be reduced, and the survival rate of the lactating pig is reduced. Studies show that sows are susceptible to uterine vaginitis and diarrhea in piglets after infection with escherichia coli, staphylococcus aureus, salmonella and streptococcus.

At present, there are reports of using traditional Chinese medicine feed additives to shorten sow labor, but the reports of the documents do not relate to the traditional Chinese medicine feed additives to shorten sow labor, which is related to the increase of sow amniotic fluid, and also do not relate to the increase of possible effective components and potential mechanisms of the sow amniotic fluid in the traditional Chinese medicine feed additives.

Disclosure of Invention

Aiming at the problems of low sheep water content in the antenatal body of the sow and low average weaned piglet number in the year in the background technology, the invention provides a fermentation compound traditional Chinese medicine additive for improving the sheep water content and PSY of the sow and a preparation method thereof.

The invention is realized by the following technical scheme:

a fermented compound traditional Chinese medicine additive for improving the water content and PSY of sows and sheep comprises the following components in parts by mass:

10-65 parts of cordate houttuynia, 5-35 parts of mugwort leaf, 6-60 parts of thymifoious euphorbia herb, 2-25 parts of cowherb seed, 1-30 parts of discolor cinquefoil herb and 10-60 parts of acalypha australis; 5-25 parts of sugar, 5-40 parts of wheat, 5-25 parts of soybean meal, 0.02-0.10 part of aspergillus niger and 0.5-2.0 parts of saccharomycetes.

Further, the fermented compound traditional Chinese medicine additive comprises the following components in parts by mass:

18-25 parts of cordate houttuynia, 20-25 parts of mugwort leaf, 32-45 parts of thymifoious euphorbia herb, 7-12 parts of cowherb seed, 20-27 parts of discolor cinquefoil herb and 35-50 parts of acalypha australis; 8-15 parts of sugar, 20-30 parts of wheat, 10-20 parts of soybean meal, 0.02-0.05 part of aspergillus niger and 1.0-1.8 parts of saccharomycetes.

Further, the traditional Chinese medicine components, wheat and bean pulp are crushed and sieved by a 80-mesh sieve.

A fermentation preparation method of a fermentation compound traditional Chinese medicine additive for improving the water content and PSY of sows and sheep comprises the following steps:

1) Weighing the raw materials according to a formula for standby;

2) Dissolving sugar, aspergillus niger and saccharomycetes in hot water, standing and uniformly stirring to obtain a mixed solution;

3) Mixing the mixed solution obtained in the step 2) with the rest raw materials, stirring uniformly, fermenting at constant temperature, taking out, vacuum sealing, and standing at room temperature for 13-17 days to obtain the fermented compound traditional Chinese medicine additive.

Further, the hot water temperature of step 2) is 40 ℃.

Further, the constant temperature fermentation temperature in the step 3) is 43 ℃ and the time is 36 hours.

Further, the fermented compound traditional Chinese medicine additive is applied to increasing the sheep water quantity in the antenatal of pregnant sows and improving the PSY of the sows.

Further, the application method comprises the following steps: from the 104 th day of pregnancy of the sow, 50g of the fermented compound traditional Chinese medicine additive is fed to each sow every day until the lactation of the sow is finished.

Compared with the prior art, the invention has the beneficial effects that:

1. the fermented compound traditional Chinese medicine additive reasonably selects various traditional Chinese medicine components according to the medicinal components and pharmacological characteristics of the traditional Chinese medicine, has various medicinal components for resisting oxidation, resisting bacteria and diminishing inflammation, protecting cardiovascular diseases, accelerating blood circulation and increasing vascular permeability, moderately increases the sheep water quantity of pregnant sows under the synergistic effect of the medicinal components, so that the birth canal is kept more lubricated, the sow birth process is shortened, piglets can be discharged from the birth canal to the outside of the birth canal more quickly, the probability of hypoxia or choking of fetuses in the birth canal is reduced, the vitality of piglets and the number of litters of the sows are improved, and in addition, the survival rate of the suckling piglets can be improved by improving the milk quality of the sows, thereby achieving the purpose of improving the number of weaned piglets per litter.

2. The plant active ingredients in the traditional Chinese medicine can play a role in inhibiting bacteria and sterilizing by inhibiting extracellular polymer production, reducing bacterial adhesion, destroying the integrity and permeability of bacterial cell membranes, inhibiting the activity of enzymes in bacteria, changing cell metabolism, inhibiting nucleic acid synthesis, enhancing animal organism immunity and the like, thereby improving the average weaned piglet number of sows.

Drawings

FIG. 1 is a schematic diagram of amniotic fluid source and balance.

Fig. 2 is a diagram of a bacteriostasis zone of a concentrated solution of the fermented compound traditional Chinese medicine feed additive A, B, C prepared by the invention.

FIG. 3 is a schematic diagram of fetal alveolar fluid and urine formation.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described in the following examples. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.

1A fermented compound Chinese medicinal feed additive composition

Example 1

Weighing 20 parts of cordate houttuynia, 23 parts of mugwort leaf, 40 parts of thymifoious euphorbia herb, 8 parts of cowherb seed, 25 parts of discolor cinquefoil herb, 45 parts of acalypha australis, 10 parts of white sugar, 26 parts of wheat, 15 parts of soybean meal, 0.03 part of aspergillus niger and 1.5 parts of saccharomycetes for standby; wherein the traditional Chinese medicine components, wheat and bean pulp are crushed and sieved by a 80-mesh sieve.

Comparative example 1

Weighing 20 parts of cordate houttuynia, 15 parts of mugwort leaf, 40 parts of thymifoious euphorbia herb, 25 parts of discolor cinquefoil herb, 45 parts of acalypha australis, 18 parts of white sugar, 8 parts of wheat, 8 parts of soybean meal, 0.05 part of aspergillus niger and 1 part of microzyme for standby; wherein the traditional Chinese medicine components, wheat and bean pulp are crushed and sieved by a 80-mesh sieve.

Comparative example 2

Weighing 25 parts of mugwort leaf, 25 parts of thymifoious euphorbia herb, 12 parts of cowherb seed, 30 parts of discolor cinquefoil herb, 30 parts of acalypha australis, 25 parts of white sugar, 10 parts of soybean meal, 0.02 part of aspergillus niger and 0.7 part of saccharomycetes for standby; wherein the traditional Chinese medicine components and the bean pulp are crushed and pass through a 80-mesh sieve.

2 fermentation of compound Chinese medicine feed additive

The total weight of the raw materials weighed in the above examples and comparative examples 1 and 2 is measured, the same weight of water is measured, the water is heated to 40 ℃, white sugar, black yeast and saccharomycetes are dissolved in hot water, the mixture is stirred uniformly after standing for 30 minutes, the obtained mixture is mixed with other raw materials and stirred uniformly, then the mixture is put into a baking oven at 43 ℃ for fermentation for 36 hours, the mixture is taken out, split charging is carried out in a vacuum bag, vacuum sealing is carried out, and the mixture is placed at room temperature for 15 days to obtain the fermented compound traditional Chinese medicine feed additive A, B, C.

3 in vitro bacteriostasis test of ferment compound Chinese medicine additive

3.1 preparation of fermented Compound Chinese medicinal additive concentrate

Opening a vacuum packaging bag, respectively weighing 50g of the fermented compound traditional Chinese medicine feed additive A, B, C into a beaker, adding 500mL of distilled water, soaking for 1 hour, heating to boiling by an electric furnace, slowly boiling by slow fire for 1 hour, standing, and filtering by using 8 layers of gauze; pouring the filter residue into a beaker, adding 300mL of distilled water, heating and slowly boiling for 1 hour, standing, filtering with 8 layers of gauze, combining the two liquid medicines, concentrating the liquid medicine to 25mL in a drying oven at 65 ℃ to ensure that the liquid medicine concentration is 2g/mL, and finally sterilizing the liquid medicine by high-pressure steam to obtain the fermented compound traditional Chinese medicine feed additive A, B, C concentrated solution. The sample was stored in a-20deg.C refrigerator for subsequent testing.

3.2 cultivation of bacterial liquid

Four bacteria were purchased from the Guangdong province microorganism collection as lyophilizates, streptococcus agalactiae (GDMCC 1.768), escherichia coli (GDMCC 1.176), staphylococcus aureus (GDMCC 1.174) and Salmonella choleraesuis subspecies choleraesuis (GDMCC 1.163). 0.3mL of brain-heart infusion (Brain Heart Infusion, BHI) broth was pipetted into a lyophilization tube containing streptococcus agalactiae lyophilized powder, gently shaken until it dissolved, the entire suspension was pipetted, inoculated into 5mL of BHI broth, and incubated in an incubator at 37℃for 48 hours. 0.3mL of Lennox Broth (LB) was taken up with a sterile pipette, dropped into a lyophilization tube containing lyophilized powder of Escherichia coli, staphylococcus aureus and Salmonella choleraesuis subsp. Suis, gently shaken until dissolved, and the whole suspension in each tube was taken up, inoculated into 5mL of LB broth, and cultured in an incubator at 37℃for 24 hours. The OD value of the bacterial liquid is measured at 660nm wavelength to make the bacterial content reach 1.5X10 ⁸ CFU/mL。

3.3 in vitro bacteriostasis test

The preparation method comprises the steps of adopting an oxford cup method, pouring sterilized tryptone soybean agar culture medium into a culture dish, uniformly coating 0.1mL of escherichia coli bacterial suspension (salmonella and staphylococcus aureus bacterial suspension are also coated according to the method) on the culture dish after the culture medium is solidified, then placing A, B and C together with 3 sterile oxford cups (7.8x6 x 10 mm) on the culture medium, weighing 200 mu L of fermented compound traditional Chinese medicine feed additive A, B and C concentrated solution, respectively placing the fermented compound traditional Chinese medicine feed additive A, B and the C concentrated solution into the corresponding oxford cups, and repeating each bacterial culture for 3 times. The petri dish was placed in a constant temperature incubator at 37 ℃ for 18 hours, and after taking out, the diameter of the inhibition zone of each liquid medicine was measured, and the average value was obtained. Pouring the sterilized blood plate culture medium into a culture dish, uniformly coating 0.1mL of streptococcus agalactiae suspension on the blood plate culture medium after the blood plate culture medium is solidified, and the rest steps are the same as the measurement steps of the colibacillus inhibition zone. The bacteriostatic effect is judged according to the following criteria: the diameter of the inhibition zone is more than 20mm and is extremely sensitive, the diameter of the inhibition zone is 15-19mm and is highly sensitive, the diameter of the inhibition zone is 10-14mm and is moderately sensitive, the diameter of the inhibition zone is less than 10mm and is low and the inhibition zone is not sensitive.

4 UHPLC-QE-MS traditional Chinese medicine non-target metabonomics detection

4.1 major instrumentation

1290-ultra high performance liquid chromatography (Agilent, usa), waters ACQUITY UPLC BEH C column (1.7 μm,2.1mm x 100 mm), Q exact Focus quadrupole tandem orbitrap high resolution mass spectrometry (Thermo Fisher Scientific, usa).

4.2 chromatographic conditions

Mobile phase: water (0.1% formic acid) and acetonitrile (0.1% formic acid), column oven temperature: 55 ℃, flow rate: 0.5mL min ^-1 Sample injection amount: 5. Mu.L. Gradient elution procedure: 0-11min,85% -25% aqueous solution; 11-12min,25% -2% aqueous solution; 12-14min,2% -2% aqueous solution; 14-14.1min,2% -85% aqueous solution; 14.1-15min,85% -85% aqueous solution; 15-16min,85% -85% aqueous solution.

4.3 Mass Spectrometry conditions

Ion source: an electrospray ionization source; alternately scanning positive and negative ions; the scan pattern is: full scan/ddMS2; mass scan range: m/z is 50-1000Da; capillary temperature: 400 ℃; spray voltage: 4000V positive ion mode and 3600V negative ion mode; gas flow rate: sheath gas 35Arb, auxiliary gas 10Arb; the parent ions are subjected to secondary fragmentation by adopting low, medium and high 3 collision energies: 15V, 30V and 45V; mass spectrum resolution: primary mass spectrum (Full Scan) 70000 FWHM (Full Width at Half Maximum), secondary mass spectrum (MS/MS) 17500 FWHM.

4.4 data processing

Extracting components of data generated by LC-QE by using XCMS software, and finally obtaining a two-dimensional data array comprising retention time, mass-to-charge ratio and peak area; importing data into SIMCA-P software (version 16.0.2, sartorius Stedim Data Analytics AB, umea, sweden), performing data processing, and establishing pattern recognition to obtain variables contributing to the packet; and performing spectrum library matching through the HMDB and the KEGG component. The MS2 score > 0.80 substance is selected as the reliable active ingredient of the traditional Chinese medicine.

5 test of feeding sow with fermented compound traditional Chinese medicine feed additive

Pregnant binary crossbred (Changbai×Dabai) sows with 20 heads, which have similar body conditions and gestational ages and are the same as those of the matched boars, are equally divided into two groups of 10 heads in each of a test group and a control group. On the 104 th day of pregnancy, 50g of the fermented compound traditional Chinese medicine feed additive A is fed to each sow of the test group every day until the lactation of the sows is finished, and the fermented traditional Chinese medicine feed additive is not fed to the sows of the control group. The amniotic fluid discharged by each sow is collected by a urine pad and weighed during sow delivery, and the time spent by each sow for farrowing, the number of born alive piglets, the weight of primary litter, the daily number of diarrhea of the suckling piglets, the death rate of the suckling piglets, the number of weaned piglets and the weight of weaned litter are recorded.

6 test results

6.1 test of antibacterial Effect of fermented Compound traditional Chinese medicine additive

As can be seen from the results of fig. 2 and table 1, the ability of the 3 fermented compound traditional Chinese medicine feed additive concentrates to inhibit the growth of escherichia coli, salmonella, staphylococcus aureus and streptococcus agalactiae is: a concentrate > B concentrate > C concentrate. The A concentrated solution has the best antibacterial effect on staphylococcus aureus, has the inferior antibacterial effect on salmonella and escherichia coli and has the worst antibacterial effect on streptococcus agalactiae, the B concentrated solution has no antibacterial effect on escherichia coli, salmonella, staphylococcus aureus and streptococcus agalactiae, and the C concentrated solution has no antibacterial effect on escherichia coli but has a certain antibacterial effect on salmonella, staphylococcus aureus and streptococcus agalactiae.

TABLE 1 in vitro bacteriostatic effect of fermented Compound traditional Chinese medicine concentrate

Note that: the difference is extremely remarkable (P < 0.01) when different capital letters exist between the same column of digital shoulder marks, the difference is remarkable (P < 0.05) when different lowercase letters exist, and the difference is not remarkable (P > 0.05) when the same letters or no letters exist. The table below is the same.

6.2 main active ingredients of the fermented Compound Chinese medicinal concentrated solution

The main active substances in the concentrated solution of the fermented compound traditional Chinese medicine feed additive A are shown in table 2.

TABLE 2 Main active ingredients of fermented Compound traditional Chinese medicine concentrate

6.3 Effect of sow supplementary feeding fermentation Compound traditional Chinese medicine additive on sow amniotic fluid and labor

The results in Table 3 show that the average amount of sheep water in the test group is significantly more than that in the control group (P < 0.05), and the average output time of piglets in the test group is also less than that in the control group (P > 0.05), which indicates that the supplementary feeding of pregnant sows with the fermented compound traditional Chinese medicine feed additive is beneficial to increasing the amount of sheep water and shortening the output time of piglets. Although the average time to yield per pig in the test group was only 1.65 minutes less than in the control group, the effect of 1.65 minutes on the piglets in the birth canal was sometimes very great, and 1.6 minutes more may lead to hypoxia or asphyxiation of the piglets and death.

TABLE 3 Effect of supplementary feeding fermented Compound traditional Chinese medicine feed additive on sheep water yield and labor

6.4 influence of sow supplementary feeding fermentation compound traditional Chinese medicine additive on sow reproductive performance

The data in Table 4 show that the average litter live piglet number, weaned piglet number and weaned piglet survival rate of the test group are respectively higher than those of the control group (P is more than 0.05), which indicates that the sow supplementary feeding fermentation compound traditional Chinese medicine feed additive is beneficial to improving the litter live piglet number and the weaned piglet survival rate, thereby improving the litter weaned piglet number of the sow.

TABLE 4 Effect of supplementary feeding fermentation Compound Chinese medicinal additives on reproductive performance of sow

6.5 influence of sow supplementary feeding fermentation compound traditional Chinese medicine additive on production performance of suckling piglets

As can be seen from Table 5, the average primary weight, average weaning weight, average daily gain and average diarrhea rate of piglets in the test group were lower than those in the control group, respectively, but there was no significant difference (P > 0.05) between the test group and the control group. The diarrhea rate of the test group of suckling pigs is lower than that of the control group, which indicates that the milk quality of the test group of sows is better than that of the control group.

TABLE 5 Effect of supplementary feeding fermentation Compound Chinese medicinal additives on production performance of suckling piglets

7 elucidating the mechanism of increasing amniotic fluid by certain Chinese medicinal components

Reducing inflammation in sows, protecting organs and blood vessels, increasing blood flow and membrane permeability are important ways to increase fetal urine and alveolar fluid production. The data in Table 2 show that the fermented compound traditional Chinese medicine feed additive contains a plurality of medicinal components with the effects of resisting oxidation, resisting bacteria, diminishing inflammation, protecting cardiovascular diseases, accelerating blood circulation and increasing vascular permeability. Ellagic acid, gallic acid, methyl gallate, ethyl gallate and kaempferide have effects of resisting oxidation and reducing injury, and can keep the morphological structure of heart, lung, kidney and blood vessel intact, and exert physiological function to the maximum extent; 7-hydroxycoumarin has diuretic effect; 7, 8-dihydroxyflavone and ellagic acid have the effects of dilating blood vessel, and increasing vascular permeability and blood flow; kaempferol, caffeic acid and salidroside have protective effect on normal development of fetal pulmonary artery blood vessel, thereby ensuring normal blood supply of lung; protocatechuic aldehyde has the functions of protecting internal organs, promoting microcirculation and dilating blood vessels; genistein has the effect of protecting glomerulus; kaempferol can alleviate nephritis and repair kidney injury. These pharmacodynamic components allow more blood to flow into the capillaries of the alveolar and glomerular surfaces, thereby producing more alveolar fluid and urine (see figure 3). Therefore, under the synergistic effect of the medicinal components, the average amniotic fluid quantity in the amniotic cavity of the pregnant sow of the test group is obviously higher than that of the control group. The moderate increase of the sheep water quantity can not only reduce the viscosity of the amniotic fluid, but also ensure that the birth canal is fully kept in a lubrication state, so that the piglets can be rapidly discharged outside the body through the birth canal under normal conditions, and the birth interval is shortened.

8 elucidate the antibacterial and anti-inflammatory effects of certain Chinese medicinal components

Studies have shown that the compounds in table 2 have various degrees of antibacterial and anti-inflammatory effects. Wherein, ellagic acid has stronger inhibition effect on staphylococcus aureus, salmonella, escherichia coli, streptococcus and bacillus cereus; purine and amino acid can inhibit the growth and reproduction of bacteria in bacteria by forming a riboswitch, thereby achieving the antibacterial and bactericidal effects; camphor inhibits salmonella growth and rejects animal endotoxin; pyrogallic acid and demethylwedelolactone have the function of inhibiting staphylococcus aureus, escherichia coli and salmonella; kaempferin, gallic acid and quercetin have good antibacterial effects on staphylococcus aureus, escherichia coli, bacillus dysenteriae, pseudomonas aeruginosa and streptococcus; the ethyl gallate can inhibit the growth of staphylococcus aureus; eriodictyol and inositol have good inhibition effects on escherichia coli, staphylococcus aureus and pseudomonas aeruginosa; the dihydroxyflavone, asiatic acid and the microorganisms such as shigella dysenteriae, salmonella, streptococcus, staphylococcus aureus, escherichia coli and the like of the isomouse Li Sudui all have good inhibiting or killing effects. The plant active ingredients play a role in bacteriostasis and sterilization mainly through the ways of inhibiting the generation of Extracellular Polymers (EPS), reducing the adhesion of bacteria, destroying the integrity and permeability of bacterial membranes, inhibiting the activity of enzymes in bacteria, changing the metabolism of cells, inhibiting the synthesis of nucleic acid, enhancing the immunity of animal bodies and the like.

9 medicinal components in the Chinese medicinal materials

The main effective components of the traditional Chinese medicine raw materials used in the embodiment 1 of the invention are shown in table 6.

TABLE 6 pharmaceutical compositions

The embodiments described above are only preferred embodiments of the present invention and are not intended to limit the present invention. Various changes and modifications may be made by those skilled in the art without departing from the spirit and principles of the invention, and it is intended that all such modifications, equivalents, and improvements fall within the scope of the invention.

Claims

1. Application of a fermented compound Chinese medicinal additive in increasing sheep water amount in pregnant sow antenatal body and improving PSY of sow;

the fermented compound traditional Chinese medicine additive is prepared from the following components:

10-65 parts of cordate houttuynia,

5-35 parts of folium artemisiae argyi,

6-60 parts of small euphorbia herb,

2-25 parts of semen vaccariae,

1-30 parts of discolor cinquefoil herb,

10-60 parts of acalypha australis;

5-25 parts of sugar, and the like,

5-40 parts of wheat, wherein the wheat is selected from the group consisting of wheat, corn, wheat bran,

5-25 parts of soybean meal,

0.02-0.10 part of Aspergillus niger,

0.5-2.0 parts of saccharomycetes.

2. The use according to claim 1, characterized in that the fermented compound traditional Chinese medicine additive is made of the following components:

18-25 parts of cordate houttuynia,

20-25 parts of folium artemisiae argyi,

32-45 parts of small euphorbia herb,

7-12 parts of semen vaccariae,

20-27 parts of discolor cinquefoil herb,

35-50 parts of acalypha australis;

8-15 parts of sugar, and the like,

20-30 parts of wheat, wherein the wheat is selected from the group consisting of wheat, corn, wheat bran,

10-20 parts of soybean meal,

0.02-0.05 part of Aspergillus niger,

1.0-1.8 parts of saccharomycetes.

3. The use according to claim 1, wherein the components of the traditional Chinese medicine, the wheat and the bean pulp are crushed and pass through a 80-mesh sieve.

4. The use according to claim 1, characterized in that the method for the fermentative preparation of the fermentative compound traditional Chinese medicine additive comprises the following steps:

1) Weighing the raw materials according to a formula for standby;

5. The use according to claim 4, wherein the hot water temperature of step 2) is 40 ℃.

6. The use according to claim 4, wherein the isothermal fermentation temperature in step 3) is 43 ℃ for 36 hours.

7. The use according to claim 1, characterized in that the application method is: from the 104 th day of pregnancy of the sow, 50g of the fermented compound traditional Chinese medicine additive is fed to each sow every day until the lactation of the sow is finished.