CN110973362A

CN110973362A - Comprehensive utilization method based on plant leaves and vinasse

Info

Publication number: CN110973362A
Application number: CN201911267280.1A
Authority: CN
Inventors: 张奎昌; 张路
Original assignee: Pizhou Gaxing Medicine Technology Service Co ltd
Current assignee: Pizhou Gaxing Medicine Technology Service Co ltd
Priority date: 2019-12-11
Filing date: 2019-12-11
Publication date: 2020-04-10

Abstract

The invention relates to a comprehensive utilization method based on plant leaves and vinasse, which comprises the steps of drying and crushing the plant leaves and the vinasse into powder, and respectively carrying out enzymolysis treatment by using cellulase, pectinase and α -amylase to respectively prepare a liquid feed additive for animals, a feed additive rich in active saccharomycetes for the animals, a solid microbial feed additive for the animals and a bio-organic fertilizer product.

Description

Comprehensive utilization method based on plant leaves and vinasse

Technical Field

The invention relates to a comprehensive utilization method based on plant leaves and vinasse. Relates to a method for preparing a feed additive for animals by carrying out enzymolysis and fermentation processes on plant leaves and vinasse substances, and then mixing, fermenting and compounding fermented waste residues to prepare a bio-organic fertilizer product.

Background

The plant resources in China are rich, along with the emphasis of China on afforestation and landscaping, the scale planting of numerous arbors, shrubs and rattan plants forms a first-class green Jiangshan, and meanwhile, the scale and facility planting industry is formed, and rich plant leaves are huge resources to be developed and utilized urgently. The plant leaves not only contain rich crude fiber, crude protein and crude fat, but also contain flavonoid, glycosides, bitter substances, saccharides, chlorophyll, vitamins, mineral substances, volatile oil and aromatic substances, and have high nutritional value, and some leaf substances also have good effect of preventing diseases. However, because the plant leaves are not well and fully utilized, a large amount of plant leaves are accumulated every year to cause great waste, and particularly after the large-scale harvest of some common crops, the accumulated fruits, vegetables and leaves, such as melon leaves and radish leaves, can be seen everywhere and can be subjected to self-ridging and rotting accumulation, so that not only is great resource waste caused, but also serious pollution is caused to the surrounding environment and water quality.

Research on efficient comprehensive utilization of plant leaves is a new subject aiming at comprehensive utilization of waste resources in rural areas at present, and the research not only changes waste resources into valuable but also bears corresponding responsibility for environmental protection. In the prior literature and patent technology inquiry, although application reports are provided for waste plants such as straws and plant leaves, the application reports have few related contents for comprehensively and comprehensively utilizing the waste plants. The invention utilizes the method of comprehensively utilizing plant leaves and vinasse, prepares products such as liquid and solid microbial feed additives rich in active saccharomycetes for animals by adopting the plant leaves and the vinasse through enzymolysis and fermentation processes, further prepares a bio-organic fertilizer product by further compounding fermented filter residues, animal manure and oil seed solid meal with compound humic acid salts after being fermented, and therefore realizes the high-efficiency and comprehensive utilization of the plant leaves. The technical scheme of the invention has no relevant literature report or patent application after search.

Disclosure of Invention

The invention aims to provide a method for preparing a corresponding product by processing a mixture formed by matching plant leaves and vinasse.

The invention utilizes the mixture of plant leaves and vinasse to prepare animal feed additives and bio-organic fertilizer products by processing, and the specific technical scheme is as follows:

based on the comprehensive utilization method of plant leaves and vinasse, according to the invention, the liquid feed additive product for animals is prepared by the following steps:

(1) picking plant leaves, drying, crushing into 80-mesh powder, adding water 30 times the weight of the plant leaves, soaking at normal temperature for 6 hours, fully stirring to obtain uniform slurry, adjusting the pH of the slurry to 4.5-5.0 by using a sulfuric acid solution, then adding cellulase with the weight of 0.75-0.95 per thousand and the activity unit of 2000 mu/g and pectinase with the activity unit of 1.25-2.5 per thousand and the activity unit of 20000 mu/g into the slurry, uniformly stirring, heating to 45-50 ℃, carrying out heat preservation and enzymolysis for 3.5-4.5 hours, and slightly stirring for 5 minutes every 60 minutes to obtain enzymolysis slurry;

(2) heating the slurry to 100 ℃, boiling for 30min, naturally cooling, standing for 18h, filtering the standing slurry to obtain a filtrate accounting for 97.5-98% of the total weight of the slurry, and taking the filtrate for later use; filtering residues for later use;

(3) drying vinasse, crushing the vinasse into 80-mesh powder, adding water 30 times the weight of the vinasse into the powder, soaking the vinasse for 4 hours, fully stirring the mixture to form uniform slurry, adjusting the pH to 5.0 by using a sulfuric acid solution, then adding cellulase with the activity unit of 2000 mu/g in a weight ratio of 0.55-0.75 thousandths, pectinase with the activity unit of 30000 mu/g in a weight ratio of 0.25-0.55 thousandths and α -amylase with the activity unit of 6000 mu/g in a weight ratio of 0.4-0.6 thousandths into the slurry, uniformly stirring the mixture, heating the mixture to 50-60 ℃, carrying out heat preservation enzymolysis for 24-30 hours, and carrying out gentle stirring for 10 minutes every 60 minutes during the enzymolysis, thus obtaining enzymolysis slurry;

(4) heating the slurry to 100 ℃, boiling for 30min, naturally cooling, standing for 24h, filtering the slurry after standing to obtain filtrate accounting for 97.2-97.8% of the total weight of the slurry, and taking the filtrate for later use; filtering residues for later use;

(5) mixing the filtrates obtained in step (2) and step (4), boiling for 30min, pouring into a fermentation barrel, sealing the barrel cover, and naturally cooling to below 35 deg.C to obtain fermentation solution;

(6) adding 0.8-1.2% of mixed bacteria into the fermentation solution obtained in the step (5), drilling a small hole in a barrel cover containing the fermentation solution, inserting a plastic hose with the inner diameter of 1.5cm, screwing the barrel cover, inserting the other end of the plastic hose into a water barrel containing clean water, and fermenting for one week at the temperature of 28-30 ℃ to obtain the fermentation solution for later use;

(7) filtering the fermentation liquor obtained in the step (6) to respectively obtain filtrate and solid-phase precipitate, wherein the solid-phase precipitate is reserved; heating and concentrating the filtrate to a concentrated solution with the specific gravity of 2.25-5.25, then adding 5% of Streptococcus lactis (Streptococcus lactis) with the viable bacteria content concentration of not less than 200 hundred million per gram and 5% of Bacillus licheniformis (Bacillus licheniformis) with the viable bacteria content concentration of not less than 200 hundred million per gram according to the weight ratio of the concentrated solution, uniformly mixing, subpackaging, and storing at the temperature of 1-2 ℃ to obtain the liquid feed additive product for animals;

continuing the following steps after the step (7) to prepare an active yeast feed additive product for animals;

(8) and (3) concentrating the concentrated solution with the specific gravity of 2.25-5.25 in the step (7) according to the following concentrated solution: yeast powder weight ratio = 1: 4, uniformly mixing, drying in vacuum at the temperature of 31 ℃, granulating by using a 12-mesh sieve, drying in vacuum at the temperature of 30 ℃ until the water content is less than 7.0 percent, and sieving by using a 16-mesh sieve to obtain the feed additive rich in the active yeast for animals.

Continuing the following steps after the step (7) to obtain a solid microbial feed additive product for animals;

(9) continuously concentrating the concentrated solution with the specific gravity of 2.25-5.25 in the step (7) into a dry extract, wherein the weight ratio of the concentrated solution to the dry extract is as follows: starch: complex microorganism = 1: 4: 1 proportion, granulating by using a 12-mesh sieve, drying by cold air, and sieving by using a 16-mesh sieve to obtain the solid microbial feed additive for animals.

According to the invention, one or more of plant leaves are crushed, and enzymolysis is carried out by using cellulase, so that plant fibers can be fully decomposed, cell walls are damaged, the dissolution of plant cell contents is increased, the content of active ingredients of the plant leaves can be improved, the cellulose can be decomposed into glucose by using the cellulase, and the nutritive value of a feed additive product is improved.

The enzymolysis liquid is further fermented by using mixed bacteria, a plurality of enzymes such as protease, lipase, amylase, chitinase and the like can be generated, B vitamins are generated to form mycoprotein, and macromolecular protein, amino acid and fat in plant leaves and vinasse can be degraded into micromolecular polypeptide and oligosaccharide, so that the protein content in the fermentation liquid reaches 11.6 percent, the protein content in the concentrated liquid reaches 22.8-28 percent, the protein content in the extract material obtained by further concentrating is as high as 38.6 percent, and the nutrition value of the plant leaf and vinasse enzymolysis liquid is reliable.

The additive containing the active yeast prepared by the invention not only can improve B vitamins and enzymes in the yeast, but also is a digestant for animals, promotes the feed intake of the animals, is beneficial to digestion and absorption, and is beneficial to the normal growth of the animals. The solid microbial feed additive for animals prepared by the invention utilizes the composite microbial bacteria which are superior to other single microbial bacteria feed additives and have stronger stress resistance, and the lactobacillus and the bacillus can quickly proliferate under the gastric acid condition and the anaerobic condition of animals, thereby ensuring that the lactobacillus and the bacillus can enter the intestines and the stomach of the animals to take effect. The yeast and the lactobacillus are cultured and fermented together in the treated slurry, on one hand, the product is used as a feed regulator and a therapeutic agent and has certain curative effect on enteritis, diarrhea, constipation, inappetence, dermatitis, urinary system infection and other diseases; as a nutritional agent, the feed additive can improve the weight gain effect of livestock and poultry, and further enhance the disease resistance and disease prevention capability of organisms. On the other hand, the product can be used as a feed additive, can improve the nutrient content of the feed, has the adjusting and treating effects of yeast and lactobacillus on livestock and poultry, and has obvious effects on adjusting the functional disorder of the digestive system or serving as a strengthening factor under the stress condition. Symbiotic culture of yeast and Bacillus (Bacillus). The bacillus has high contents of protease, lipase and amylase, and has strong degradation capability on plant carbohydrates. According to the biological characteristics of the used strains, the feed additive is dried at low temperature and can improve the growth rate of livestock by 3.8 percent. The yeast, the lactic acid bacteria and the bacillus are mixed according to a proportion, so that the effect is better, and the yeast, the lactic acid bacteria and the bacillus are not only used as a feeding nutrient and a feed additive, but also used as a feeding therapeutic agent, and can partially replace antibiotics to be used for treating digestive system diseases. The animal liquid feed additive, the active yeast feed additive and the solid microbial feed additive respectively prepared by the invention have diversity for feeding animals, are beneficial to feed with mixed materials, provide convenience for feeding as drinking water, and have the effects of supplementing nutrition, promoting digestion, helping the treatment of animals and accelerating the recovery of organisms by using the product to be mixed with water for drinking and feeding for sub-health animals suffering from diseases or insufficient digestion.

The additive product prepared by the invention has the functions of increasing the nutrition of animals, promoting digestion, improving the utilization rate of feed and promoting the growth of animals. The animals to be used in the present invention include livestock such as cattle, sheep, pigs, rabbits, poultry such as chickens, ducks, geese, etc., and aquatic animals such as fishes, shrimps, crabs, chelates, etc. The liquid feed additive for animals can be added into water according to the proportion of 0.25-0.5% for livestock or poultry to drink freely; can also be expressed per m³0.5kg of the fertilizer is thrown into a fish pond to improve the nutritional structure of water quality. The feed additive product rich in active saccharomycetes for animals can be added into a livestock compound feed or a mixed ration according to the proportion of 0.5-1.0% for application; adding the feed into poultry compound feed or mixed ration according to the proportion of 1.0-2.0% for application; 0.5-1.5% of the additive is added into aquatic animal feed for application. The solid microbial feed additive product for animals can be added into a livestock compound feed or a mixed ration according to the proportion of 0.25-0.5 percent for application; adding the feed into poultry compound feed or daily ration according to the proportion of 1.0-2.0 percent; 0.5-1.5% of the additive is added into aquatic animal feed for application.

After the step (2), the following steps are continued to prepare the bio-organic fertilizer product:

(10) combining the filter residue obtained in the step (2) and the filter residue obtained in the step (4) with the solid-phase precipitate obtained in the step (7)

Mixing to obtain mixed filter residue material;

(11) mixing the filter residue mixed material obtained in the step (10) with the dried animal manure, the oil seed solid meal and the composite bacteria starter in a weight percentage of 25-35% of the filter residue mixture, 42-60% of the dried animal manure, 7-13% of the oil seed solid meal and 8-15% of the composite bacteria starter, and adding a proper amount of water to adjust the water content to 40-45% to obtain a solid culture base material;

(12) putting the solid culture base material prepared in the step (11) into a fermentation tank for fermentation for 40-45 days, turning the pile in time when the fermentation temperature rises to 40 ℃, avoiding overhigh temperature, pouring out of the fermentation tank after the fermentation is finished, airing, crushing, and sieving by a sieve of 50-60 meshes to obtain fermented material powder;

(13) and (3) mixing the fermented material powder obtained in the step (12) and the composite humate according to the mass ratio of 82-90% of the fermented material powder to 10-18% of the composite humate, uniformly mixing, and then carrying out batching, granulation, screening and packaging according to a conventional method to obtain the bio-organic fertilizer product.

According to the thought of the invention, the aim is to obtain the animal feed additive by carrying out enzymolysis, fermentation and other treatments on plant leaves and vinasse, and further process and treat filter residues (wet materials) and solid-phase precipitates (wet materials) generated by the animal feed additive to obtain the beneficial microbial organic fertilizer. The main raw materials of the biological organic fertilizer prepared by the method are a basic material base consisting of filter residue and solid-phase precipitate for preparing the feed additive for animals, oil seed solid meal substances and dried animal manure, and the basic material base is fermented by a compound bacteria fermenting agent consisting of saccharomycetes, lactic acid bacteria and bacillus, so that organic components in the material base can be beneficially and fully converted, particularly, the contents of amino acid, B vitamins and active microbial bacteria are increased, the vitamins, amino acid, protein, nucleic acid, inorganic substances, easily-oxidized organic matters, sugar, crude fat, active microbial bacteria and the like in the product are enriched, particularly, the protein which cannot be directly utilized by plants is beneficially converted through long-term fermentation biochemical conversion, the protein components are converted into the amino acid, further, the amino acid is converted into small peptide or short peptide, and the biological efficiency of the organic fertilizer is improved, the use effect is improved. The characteristic that sodium, potassium and ammonium humate are easy to dissolve in water is utilized in the preparation of the bio-organic fertilizer, so that the bio-organic fertilizer has quick application effect and strong fertility, the organic matter capacity of the bio-organic fertilizer treated by the technical scheme of the invention is high and can reach more than 78 percent, and the bio-organic fertilizer is rich in nutrition, is rich in protein, amino acid, carbohydrate, B vitamins, active microbial bacteria, mycoprotein and mineral trace elements, and is suitable for fertilizer selection in green planting production.

The filter residue mixed material is a wet material, and the weight percentage of the filter residue mixed material is the weight percentage of the wet material.

According to the present invention, "%" which is not specifically designated in the present invention is a weight percentage.

According to the invention, the technical scheme is further optimized and/or selected:

the plant leaf in the step (1) is pumpkin leaf or watermelon leaf or white gourd leaf or cucumber leaf or towel gourd leaf or melon leaf or north melon leaf or cucurbit leaf or gourd leaf or garlic leaf or ginger leaf or leek leaf or welsh onion leaf or celery leaf or cauliflower leaf or dried moss leaf or lettuce leaf or radish leaf or carrot leaf or mustard leaf or chilli leaf or eggplant leaf or bud leaf or pea leaf or cowpea leaf or hyacinth bean leaf or broad bean leaf or soybean leaf or red bean leaf or sesame leaf or cassava leaf or burdock leaf or cloth residue leaf or lotus leaf or water chestnut leaf or tomato leaf or sweet potato leaf or chicory leaf or jerusalem artichoke leaf or kochia leaf or cabbage leaf or spinach leaf or water chestnut leaf or taro leaf or oat leaf or tea leaf or yam leaf or common yam leaf or beef leaf or argy wormwood leaf or clover leaf or leech leaf or asparagus leaf or purple sweet potato leaf or asparagus leaf or purple sweet potato leaf or black leaf or purple sweet potato Or folium Ginseng or radix Codonopsis leaf or folium Angelicae sinensis or folium Cassiae or folium Ophiopogonis or radix Salviae Miltiorrhizae leaf or folium Fagopyri Tatarici or folium Hordei Germinatus or folium Tritici Aestivi or herba Taraxaci leaf or herba Houttuyniae leaf or herba Schizonepetae or folium Carthami tinctorii or caulis Lonicerae or folium Ginkgo or folium Aloe or folium Polygoni Multiflori or folium Amorphophalli or folium Rubi Parvifolii or caulis et folium Puerariae Lobatae or folium Glycyrrhizae or folium Jujubae or folium Elaeagni Angustifoliae or folium Hippophae or folium Lycii or folium Persicae or folium Armeniacae amarum or folium Pini or folium Psidii Guajavae or folium Momordicae Charantiae or folium Paeoniae alba or folium Ilicis Purpureae or folium Chrysanthemi Indici or folium fici or folium Ilicis or folium Castaneae or folium Pruni Bidentis or folium Mali Pumilae or folium Mali or folium Broussonetiae or folium Brassica chinensis or caulis et folium Brassicae or caulis Opuntiae or folium Eucalypti Globueli or folium Chrysanthemi or folium Melissae or folium et folium Opuntiae or folium Chrysanthemi or folium Ponciri or folium Bean leaf or white atractylodes rhizome leaf or arborvitae leaf or mint leaf or psoralea fruit leaf or atractylodes rhizome leaf or red peony root leaf or acanthopanax leaf or lophatherum gracile leaf or finger citron leaf or galangal rhizome leaf or rhodiola root leaf or mangnolia officinalis leaf or fenugreek seed leaf or sophora japonica leaf or sealwort leaf or asiatic pennywort leaf or gynostemma pentaphyllum leaf or platycodon grandiflorum leaf or tartarian buckwheat leaf or papaya leaf or myrtle leaf or ligustrum japonicum leaf or privet leaf or astragalus membranaceus leaf or madder leaf or cardamom leaf or mulberry leaf or sour bean leaf or asparagus cochinchinensis leaf or acanthopanax bark leaf or common cephalum leaf or motherwort leaf or alpinia oxyphylla leaf or sweet stevia leaf or rubus leaf or polygala leaf or bilberry leaf or sour orange leaf or caulis cirrhosae leaf or senna leaf or common anemarrhena leaf or black ginseng leaf or phellodendron amurense leaf or rhubarb leaf or bupleurum leaf or cyrtomium rhizome leaf or forsythia fruit leaf or bitter orange leaf or vally leaf or gentian leaf or yellow sophora root leaf or common fennel leaf or green bean leaf or common fennel leaf or common fen One or two or more of euphorbia pekinensis leaf, negundo chastetree leaf, sanguisorba officinalis leaf and pomegranate leaf can be combined randomly.

The pumpkin leaf or watermelon leaf or white gourd leaf or cucumber leaf or towel gourd leaf or melon leaf or northern melon leaf or cucurbit leaf or gourd leaf or garlic leaf or ginger leaf or leek leaf or welsh onion leaf or celery leaf or cauliflower leaf or dried moss leaf or lettuce leaf or carrot leaf or mustard leaf or chili leaf or eggplant leaf or sprout leaf or pea leaf or cowpea head leaf or hyacinth bean leaf or broad bean leaf or red bean leaf or sesame leaf or cassava leaf or burdock leaf or cloth residue leaf or lotus leaf or water chestnut leaf or sweet potato leaf or chicory leaf or jerusalem artichoke leaf or kochia leaf or sweet potato leaf or garlic leaf or spinach leaf or water chestnut leaf or fur leaf or taro leaf or oat leaf or tea leaf or potato leaf or asparagus leaf or safflower leaf or dogwood leaf or asparagus leaf or safflower leaf or common or asparagus leaf or purple sweet potato leaf or common yam leaf or purple sweet potato leaf or purple flower leaf or purple leaf or Angelica sinensis leaf or Cassia tora leaf or ophiopogon japonicus leaf or salvia miltiorrhiza leaf or tartary buckwheat leaf or barley leaf or wheat leaf or dandelion leaf or houttuynia cordata leaf or schizonepeta leaf or safflower leaf or honeysuckle leaf or ginkgo leaf or aloe leaf or polygonum multiflorum leaf or konjak leaf or purslane stem leaf or pueraria lobata leaf or liquorice leaf or jujube leaf or russianolive leaf or wild jujube leaf or olea europaea leaf or echinacea leaf or fructus hippophae leaf or fructus lycii leaf or peach leaf or apricot leaf or pine needle leaf or guava leaf or momordica grosvenori leaf or peony leaf or holly leaf or chrysanthemum leaf or fig leaf or chestnut leaf or fructus physali leaf or apple leaf or pear leaf or Broussonetia leaf or potato leaf or cane leaf or mango leaf or eucommia bark leaf or holly leaf or elm leaf or eucalyptus leaf or persimmon leaf or loquat leaf or artemisia japonica leaf or artemisia japonica leaf or asparagus leaf or atractylodes macrocephala white fungus leaf or asparagus leaf or yellow mea Leaf or mint leaf or psoralea fruit leaf or atractylodes rhizome leaf or red peony root leaf or acanthopanax leaf or lophatherum gracile or finger citron leaf or lesser galangal rhizome leaf or rhodiola root leaf or magnolia bark leaf or fenugreek seed leaf or sophora flower leaf or polygonatum rhizome leaf or asiatic pennywort leaf or gynostemma pentaphyllum leaf or platycodon grandiflorum leaf or fagopyrum cymosum leaf or rose leaf or papaya leaf or geranium leaf or privet leaf or astragalus membranaceus leaf or madder leaf or cardamon leaf or mulberry leaf or sour bean leaf or asparagus leaf or acanthopanax bark leaf or common cephalanoplos leaf or motherwort leaf or alpinia oxyphylla leaf or stevia rebaudiana leaf or yunnanensis leaf or cowberry leaf or sour orange leaf or citrus reticulata leaf or vine leaf or senna leaf or rhizoma anemarrhenae leaf or brown ginseng leaf or phellodendron bark leaf or barberry leaf or morinda officinalis leaf or rhubarb leaf or bupleurum leaf or cyrtomium rhizome leaf or bitter ginseng leaf or forsythia fruit leaf or stemona leaf or valsa leaf or gentian leaf or yellow sophora leaf or yellow meadowrue leaf or common clubmoss herb leaf or yellow sophora leaf or common Folium Ulmi Pumilae or folium Granati is dry or fresh leaf; preferably, the application is dry leaves.

The vinasse in the step (3) is one or a mixture of dry white spirit vinasse, dry yellow wine vinasse, dry brewer's grain, dry alcohol grain [ DDG ], dry alcohol grain soluble substance [ DDS ], dry whole alcohol grain [ DDGS ], wet alcohol grain [ DWG ] and wet alcohol grain soluble substance [ DWS ].

The dry alcohol tank [ DDG ] is any one of a barley dry alcohol tank, a rice dry alcohol tank, a corn dry alcohol tank, a sorghum dry alcohol tank, a wheat dry alcohol tank, a rye dry alcohol tank, a cereal dry alcohol tank and a potato dry alcohol tank sold in the market.

The dry alcohol tank soluble substance [ DDS ] is any one of a barley dry alcohol tank soluble substance, a rice dry alcohol tank soluble substance, a corn dry alcohol tank soluble substance, a sorghum dry alcohol tank soluble substance, a wheat dry alcohol tank soluble substance, a rye dry alcohol tank soluble substance, a grain dry alcohol tank soluble substance and a potato dry alcohol tank soluble substance which are sold in the market.

The dry total distillers ' grains [ DDGS ] are any one of barley dry distillers ' grains containing soluble substances, rice dry distillers ' grains containing soluble substances, corn dry distillers ' grains containing soluble substances, sorghum dry distillers ' grains containing soluble substances, wheat dry distillers ' grains containing soluble substances, rye dry distillers ' grains containing soluble substances, cereal dry distillers ' grains containing soluble substances and potato dry distillers ' grains containing soluble substances which are sold in the market.

The wet distillers ' grains [ DWG ] are any one of barley wet distillers ' grains, rice wet distillers ' grains, corn wet distillers ' grains, sorghum wet distillers ' grains, wheat wet distillers ' grains, rye wet distillers ' grains, cereal wet distillers ' grains and potato wet distillers ' grains which are available in the market.

The wet distillers ' grains soluble substance [ DWS ] is any one of barley wet distillers ' grains soluble substance, rice wet distillers ' grains soluble substance, corn wet distillers ' grains soluble substance, sorghum wet distillers ' grains soluble substance, wheat wet distillers ' grains soluble substance, rye wet distillers ' grains soluble substance, grain wet distillers ' grains soluble substance and potato wet distillers ' grains soluble substance which are commercially available.

The grain stillage products described above are products obtained from two or more kinds of grain seeds, such as grain dry distillers ' grains, grain dry distillers ' grains solubles, grain dry distillers ' grains containing solubles, grain wet distillers ' grains, and grain wet distillers ' grains solubles.

The drying in the step (3) can be selected from hot air drying, far infrared drying and microwave drying, and preferably microwave drying is applied.

The mixed bacteria in the step (6) are prepared from saccharomycetes (Saccharomyces) with viable bacteria concentration of not less than 200 million per gram, lactobacillus (Lactobacillus) with viable bacteria concentration of not less than 200 million per gram and Bacillus (Bacillus) with viable bacteria concentration of not less than 200 million per gram according to the weight ratio: lactic acid bacteria: bacillus = 2: 1.5: 1.5 ratio.

The yeast (Saccharomyces) can be selected from any one of Candida utilis (Candida utilis), brewer's yeast (Saccharomyces cerevisiae), red yeast (Rhodotorula), beer yeast (Saccharomyces cerevisiae) or feed yeast (feed yeast) with viable bacteria concentration of not less than 200 million per gram.

The Lactobacillus (Lactobacillus) is one or more of Lactobacillus fermentum (Lactobacillus fermentum), Streptococcus lactis (Streptococcus lactis), Lactobacillus lactis (Lactobacillus lactis), Lactobacillus plantarum (Lactobacillus plantarum) and Lactobacillus bulgaricus (Lactobacillus bulgaricus) in any combination.

The Bacillus (Bacillus) is one or more of Bacillus licheniformis (Bacillus licheniformis), Bacillus lentus (Bacillus lentus), Bacillus pumilus (Bacillus pumilus), Bacillus subtilis (Bacillus subtilis) and Bacillus coagulans (Bacillus coagulogenins) which are combined optionally.

As for the yeast, the lactic acid bacteria and the bacillus, the yeast, the lactic acid bacteria and the bacillus dry powder products of which the viable bacteria concentration per gram is not less than 200 hundred million in the edible or feed sold in the market can be selected and used.

As for the dry powder preparation of yeast, lactic acid bacteria and bacillus, commercially available products can be selected, and it is not difficult to obtain the dry powder preparation by knowing the growth properties of the bacteria in the prior art, which is well known to those skilled in the art. For example, the respective strains are individually selected from the preserved strains (e.g., cryopreserved) by a conventional method, streaked and inoculated into a solid plate medium, cultured under appropriate conditions, and colonies are grown, and then inoculated into a correspondingly optimized liquid medium by using a liquid culture fermentation apparatus for shake culture, and the culture conditions (e.g., temperature, etc.) can be found in relevant manuals, such as manuals or textbooks on the culture of microorganisms.

In order to be suitable for the fermentation treatment of the material of the invention. The preparation method comprises the steps of respectively culturing Candida utilis, brewer's yeast, red yeast, beer yeast, feed yeast, lactobacillus fermentum, streptococcus lactis, lactobacillus plantarum, lactobacillus bulgaricus, bacillus subtilis, bacillus licheniformis, bacillus coagulans, bacillus lentus and bacillus pumilus in respective independent culture systems by adopting liquid culture fermentation equipment through an optimized culture medium, separating thalli in fermentation liquor by using a high-speed centrifuge, and drying bacterial sludge in a drying chamber in a cold air drying or vacuum freeze drying mode to obtain dry thalli products with viable bacteria concentration of not less than 200 billion per gram respectively, wherein the dry thalli products can be used independently according to formula requirements or can be mixed according to a proportion for use.

The yeast, the lactobacillus and the bacillus are dry powder products of which the viable bacteria concentration is not less than 200 hundred million per gram.

The yeast powder in the step (8) is any one of beer yeast dry powder products, candida dry powder products and feed yeast dry powder products, wherein each gram of yeast powder contains not less than 200 hundred million viable bacteria.

The composite microorganism bacterium in the step (9) is prepared from yeast dry powder products with viable bacteria concentration of not less than 200 hundred million per gram, lactobacillus dry powder products with viable bacteria concentration of not less than 200 hundred million per gram and bacillus dry powder products with viable bacteria concentration of not less than 200 hundred million per gram according to the weight ratio of yeast: lactic acid bacteria: bacillus = 2: 1: 1 ratio.

The yeast can be any one of Candida utilis (Candida utilis), cerevisiae fermentum (cerevisiae) or feed yeast (feed yeast) which is available in the market and used as a food additive or a feed additive and has the viable bacteria concentration of not less than 200 hundred million per gram; it is preferable to use a feed yeast (feed yeast).

The Bacillus (Bacillus) is one or more of Bacillus subtilis, Bacillus licheniformis (Bacillus licheniformis), Bacillus coagulans (Bacillus coagulosus), Bacillus stearothermophilus (Bacillus stearothermophilus) and Bacillus firmus (Bacillus firmus) which are optionally combined.

The lactic acid bacteria (Lactobacillus) may be one or more of Streptococcus thermophilus, Lactobacillus plantarum, Lactococcus lactis and Lactobacillus bulgaricus.

The Bacillus (Bacillus) and the lactic acid bacteria (Lactobacillus) can be selected from all commercially available dry powder products of the bacteria with the viable bacteria concentration of not less than 200 hundred million per gram, which are used as food additives or feed additives.

The dry powder products of yeast, bacillus and lactobacillus with the viable bacteria concentration of not less than 200 billion per gram are not only selected from commercially available edible or feeding viable bacteria products, but also can be obtained easily by knowing the growth properties of the bacteria in the prior art according to the knowledge of the skilled person. For example, the respective strains are individually selected from the preserved strains (e.g., cryopreserved) by a conventional method, streaked and inoculated into a solid plate medium, cultured under appropriate conditions, and colonies are grown, and then inoculated into a correspondingly optimized liquid medium by using a liquid culture fermentation apparatus for shake culture, and the culture conditions (e.g., temperature, etc.) can be found in relevant manuals, such as manuals or textbooks on the culture of microorganisms.

In order to be suitable for the fermentation treatment of the material of the invention. Candida utilis, beer yeast, feed yeast, bacillus subtilis, bacillus licheniformis, bacillus coagulans, bacillus stearothermophilus, bacillus firmus, streptococcus thermophilus, lactobacillus plantarum, lactococcus lactis and lactobacillus bulgaricus can be respectively cultured in independent culture systems by optimized culture media and liquid culture fermentation equipment, then thalli in fermentation liquor are separated by a high-speed centrifuge and are dried in a drying chamber by a cold air drying or vacuum freeze drying mode to obtain thalli dry powder products with viable bacteria concentration of not less than 200 billion per gram, and the thalli dry powder products can be used independently according to formula requirements and can also be mixed according to proportions.

The animal manure in the step (11) is dried at 150 ℃.

The animal manure in the step (11) is any one or a mixture of any combination of sheep manure, pig manure, cow manure, horse manure, donkey manure, camel manure and rabbit manure; preferably sheep manure, cow manure, rabbit manure or any mixture thereof.

The oil seed meal in the step (11) is one of almond meal, rapeseed meal, double-low rapeseed meal, soybean germ meal, soybean meal, tomato seed meal, olive meal, walnut kernel meal, pecan kernel meal, safflower seed meal, pepper seed meal, peanut meal, sunflower seed meal, cottonseed meal, wood cottonseed meal, grape seed meal, sea buckthorn seed meal, wild jujube meal, shinyleaf yellowhorn meal, linseed meal, coconut meal, palm seed meal, evening primrose seed meal, sesame meal, perilla seed meal, capsicum seed meal, corn germ meal, wheat germ meal and rice bran meal or any mixture thereof.

The composite bacteria starter in the step (11) is prepared by mixing lactic acid bacteria (lactococcus) with the viable bacteria content of not less than 200 hundred million per gram, Bacillus (Bacillus) with the viable bacteria concentration of not less than 200 million per gram and yeast (saccharomyces) with the viable bacteria concentration of not less than 200 million per gram according to a proportion, wherein the preferred weight ratio is lactic acid bacteria: bacillus: yeast = 1: 1: 1 ratio.

The lactic acid bacteria are selected from one or more of Streptococcus thermophilus (Streptococcus thermophilus), Streptococcus lactis (Streptococcus acidicii), Lactobacillus acidophilus (Bacillus acidophilus), Lactobacillus delbricikii and Lactobacillus reuteri (Lactobacillus rentdrini) in any combination.

The Bacillus is one or more of Bacillus licheniformis (Bacillus licheniformis), Bacillus circulans (Bacillus circulans Jordan), Bacillus subtilis (Bacillus subtilis), Bacillus firmus (Bacillus firmus) and Bacillus lentus (Bacillus lentus) which are used in the Bacillus.

The yeast may be any one of brewers' yeast (Saccharomyces cerevisiae), Candida utilis (Candida utilis) and Candida tropicalis (Candida tropicalis).

The lactic acid bacteria, the bacillus and the microzyme are dry powder products of which the viable bacteria concentration per gram is not less than 200 hundred million.

The lactobacillus, the bacillus and the yeast can be selected from lactobacillus, bacillus and yeast dry powder products which are applied to commercial biological fertilizers or feeds and contain not less than 200 hundred million viable bacteria per gram.

The lactic acid bacteria, bacillus and yeast described above can also utilize known strains of the prior art, such as those deposited in the general microbiological culture collection center of the academy of sciences of china and the collection centers of other branches or other strains available from the prior art. The composite bacteria starter can be independently cultured in advance and then mixed when in use;

the lactic acid bacteria, bacillus and yeast described above can be prepared by using the known strains in the prior art, such as those deposited in the center for general microbiological culture collection of the academy of sciences of China and the collection centers of other branches, or other strains available in the prior art. The composite bacteria starter can be independently cultured in advance and then mixed when in use; the dry powder preparation of the lactic acid bacteria, the bacillus and the yeast with the viable bacteria concentration of not less than 200 hundred million per gram is not difficult to obtain by knowing the growth property of the biological bacteria according to the prior known technology. For example, the respective strains are individually selected from the preserved strains (e.g., cryopreserved) by a conventional method, streaked and inoculated into a solid plate medium, cultured under appropriate conditions to grow colonies, and then inoculated into a correspondingly optimized liquid medium by using a liquid culture fermentation apparatus for shake culture, and the culture conditions (e.g., temperature, etc.) can be found in relevant manuals, such as manuals or textbooks on the culture of microorganisms.

In order to be suitable for stabilizing the microbial bacteria added into the material of the invention and ensure the effective survival rate of the microbial bacteria in the product, can respectively culture streptococcus thermophilus, streptococcus lactis, lactobacillus acidophilus, lactobacillus delbrueckii, lactobacillus reuteri, bacillus licheniformis, bacillus circulans, bacillus subtilis, bacillus firmus, bacillus lentus, brewer's yeast, candida utilis and candida tropicalis in respective independent culture systems by adopting a liquid culture fermentation device through an optimized culture medium, and then separating out the thalli in the fermentation liquor by high-speed centrifugation, placing the thalli in a drying chamber, and drying the bacterial sludge in a cold air drying or vacuum freeze drying mode to obtain the thalli dry powder products with viable bacteria concentration of not less than 200 hundred million per gram, wherein the thalli dry powder products can be used independently according to formula requirements or can be mixed according to a proportion for use.

The compound humate in the step (13) is sodium humate, potassium humate and ammonium humate, and the weight ratio of the compound humate to the compound humate is sodium humate: potassium humate: ammonium humate = 1: 1.5: 2, and mixing the components in a ratio of 2.

The above-mentioned equipments of the present invention are all equipments conventional in the art.

The process steps of the invention do not describe in detail the operation of the process in general knowledge of the person skilled in the art; the particular equipment not specifically illustrated in the process is well within the skill of the art and alternative conventional equipment is well within the skill of those in the art.

The technical scheme of the invention has the following beneficial effects:

1. the method comprises the steps of mixing plant leaf substances with certain nutrition and/or health care functions with vinasse substances rich in nutrients such as crude protein, crude fat and crude fiber, respectively preparing a liquid feed additive for animals, a feed additive rich in active saccharomycetes and a solid microbial feed additive for animals through enzymolysis and mixed bacteria fermentation, fermenting wet residues, dried animals and oil seed solid meal through a compound bacteria starter, and then compounding compound humate to prepare a bio-organic fertilizer product, thereby forming an industrial chain for comprehensive utilization of raw materials, providing a new method, a process and a technical route for fully utilizing comprehensive resources of plant leaves and byproducts, providing new nutrition and health care products for animal feeding and providing organic microbial fertilizers for green planting industry, providing good social benefits for production and processing enterprises and providing waste resource utilization for production and processing enterprises, a new method and idea are found for improving the economical and efficient utilization of waste resources, and meanwhile, the economic benefit of enterprises is greatly improved.

2. The method is implemented by using plant leaves and vinasse substances to be mixed and fermented, and the multi-class feed additive for animals prepared by the previous stage process is further fermented to prepare the bio-organic fertilizer from waste residues after enzymolysis, so that no waste is generated in the process, the utilization effect of 'eating, drying and squeezing out' of raw materials is realized, the comprehensive utilization of raw material resources is realized, waste is turned into wealth, the environmental pollution is reduced, the prepared product has higher nutritional effect, the method has remarkable economic and social benefits for promoting pollution-free cultivation of animals (such as livestock and poultry) and promoting agricultural green planting, and has better boosting effect on industrial sustainable development.

Detailed Description

Example 1

A comprehensive utilization method based on plant leaves and vinasse comprises the following steps:

(1) picking and selecting the pumpkin leaves and the dandelion leaves of the plant leaves, respectively drying, and mixing the pumpkin leaves and the dandelion leaves according to the weight ratio of 10: 1 proportion, crushing into 80-mesh powder, weighing 100kg of powder, placing into a reaction kettle, adding 3000kg of purified water, soaking at normal temperature for 6 hours, fully stirring to obtain uniform slurry, adjusting the pH of the slurry to 4.5 by using a sulfuric acid solution, then adding 2945g of cellulase with the activity unit of 2000 mu per gram and 7750g of pectinase with the activity unit of 20000 mu per gram into the slurry, uniformly stirring, heating to 45 ℃, preserving heat for enzymolysis for 4.5 hours, and slightly stirring for 5 minutes every 60 minutes to obtain enzymolysis slurry;

(2) heating the slurry prepared in the step (1) to 100 ℃, boiling for 30min, naturally cooling, standing for 18h, filtering the standing slurry to obtain 3038kg of filtrate, taking the filtrate for later use, and filtering the residue for later use;

(3) after the dry white spirit vinasse is subjected to microwave drying treatment, crushing the dried white spirit vinasse into 80-mesh powder, weighing 100kg of the powder, placing the powder into a reaction kettle, adding 3000kg of purified water, soaking for 4 hours, fully and uniformly stirring to obtain uniform slurry, adjusting the pH to 5.0 by using a sulfuric acid solution, then adding 2325g of cellulase with the activity unit of 2000 mu m per gram, 1705g of pectinase with the activity unit of 30000 mu m per gram and 1240g of α -amylase with the activity unit of 6000 mu m per gram into the slurry, uniformly stirring, heating to 50 ℃, carrying out heat preservation and enzymolysis for 30 hours, and slightly stirring for 10 minutes every 60 minutes during the heating to obtain enzymolysis slurry;

(4) heating the slurry prepared in the step (3) to 100 ℃, boiling for 30min, naturally cooling, standing for 24h, filtering the slurry after standing to obtain 3013.2kg of filtrate, taking the filtrate for later use, and filtering the residue for later use;

(5) mixing the filtrates obtained in step (2) and step (4), boiling for 30min, pouring into a plastic fermentation barrel, sealing the barrel cover, and naturally cooling to below 35 deg.C to obtain fermentation solution;

(6) adding the fermentation solution to be fermented in the step (5) into brewing yeast in a weight ratio of: lactobacillus fermentum: bacillus licheniformis = 2: 1.5: 48.41kg of mixed bacteria in a proportion of 1.5, drilling a small hole in a barrel cover containing liquid to be fermented, inserting a plastic hose with the inner diameter of 1.5cm, screwing the barrel cover, inserting the other end of the plastic hose into a water barrel containing clean water to facilitate the discharge of gas in the fermentation liquid, and fermenting for one week at the temperature of 28-30 ℃ to obtain the fermentation liquid for later use;

(7) filtering the fermentation liquor obtained in the step (6) to respectively obtain filtrate and solid-phase precipitate, wherein the solid-phase precipitate is reserved; heating and concentrating the filtrate to a concentrated solution with the specific gravity of 2.25-5.25, then adding 5% of streptococcus lactis with the viable bacteria concentration of not less than 200 hundred million per gram and 5% of bacillus licheniformis with the viable bacteria concentration of not less than 200 hundred million per gram according to the weight ratio of the concentrated solution, uniformly mixing, subpackaging, and storing at the temperature of 1-2 ℃ to obtain the liquid feed additive product for animals.

And (3) continuing the following steps after the step (7) to prepare the feed additive product rich in the active yeast for the animals:

(8) and (3) concentrating the concentrated solution with the specific gravity of 2.5-5.25 in the step (7) according to the following concentrated solution: the feed yeast powder weight ratio = 1: 4, uniformly mixing, drying in vacuum at the temperature of 31 ℃, granulating by using a 12-mesh sieve, drying in vacuum at the temperature of 30 ℃ until the water content is less than 7.0 percent, and sieving by using a 16-mesh sieve to obtain the feed additive rich in the active yeast for the animals;

and (3) continuing the following steps after the step (7) to prepare a solid microbial feed additive product for animals:

(9) continuously concentrating the concentrated solution with the specific weight of 2.25-5.25 in the step (7) into a dry extract, wherein the dry extract comprises the following components in parts by weight: starch: complex microorganism = 1: 4: 1, wherein the composite microbial bacteria are prepared from feed yeast dry powder with 200 hundred million per gram, streptococcus thermophilus dry powder with 200 hundred million per gram and bacillus licheniformis dry powder with 200 hundred million per gram according to the weight ratio of 2: 1: 1, granulating by using a 12-mesh sieve, drying by cold air, and sieving by using a 16-mesh sieve to obtain the solid microbial feed additive for animals;

(10) mixing the filter residues obtained in the steps (2) and (4) and the solid-phase precipitate obtained in the step (7) uniformly to obtain a filter residue mixed material for later use;

(11) taking 25kg of filter residue mixed material obtained in the step (10), mixing the filter residue mixed material with 60kg of dried rabbit manure and 7kg of rapeseed meal, and then adding the dried powder of streptococcus lactis with the viable bacteria concentration of 200 hundred million per gram, the dried powder of bacillus circulans with the viable bacteria concentration of 200 hundred million per gram and the dried powder of brewer's yeast with the viable bacteria concentration of 200 hundred million per gram according to the weight ratio of 1: 1: 8kg of composite bacteria starter mixed according to the proportion of 1, uniformly mixing, adding a proper amount of water to adjust the water content to 40-45 percent, and obtaining a solid culture base material;

(12) putting the solid culture base material prepared in the step (11) into a fermentation tank for fermentation for 45 days, turning the fermentation tank in time when the fermentation temperature rises to 40 ℃, avoiding overhigh temperature, pouring the fermented material out of the fermentation tank after the fermentation is finished, airing, crushing, and sieving by a 50-mesh sieve to obtain fermented material powder;

(13) taking 82kg of the fermented material powder obtained in the step (12), mixing with sodium humate, potassium humate and ammonium humate according to the weight ratio of 1: 1.5: and mixing 18kg of the composite humate obtained by mixing 2 weight ratios uniformly, and then carrying out batching, granulation, screening and packaging according to a conventional method to obtain the bio-organic fertilizer product.

Example 2

(1) picking and selecting watermelon leaves, garlic leaves and eggplant leaves of plant leaves, respectively drying, and mixing the watermelon leaves, the garlic leaves and the eggplant leaves according to the weight ratio of 10: 3: crushing the powder into 80-mesh powder according to a proportion of 6, weighing 100kg of the powder, placing the powder into a reaction kettle, adding 3000kg of purified water, soaking for 6 hours at normal temperature, fully stirring to obtain uniform slurry, adjusting the pH of the slurry to 5.0 by using a sulfuric acid solution, adding 2325g of cellulase with the activity unit of 2000 mu per gram and 3875g of pectinase with the activity unit of 20000 mu per gram into the slurry, uniformly stirring, heating to 50 ℃, preserving heat for enzymolysis for 3.5 hours, and slightly stirring for 5 minutes every 60 minutes during the period to obtain enzymolysis slurry;

(2) heating the slurry prepared in the step (1) to 100 ℃, boiling for 30min, naturally cooling, standing for 18h, filtering the standing slurry to obtain 3022.5kg of filtrate, taking the filtrate for later use, and filtering the residue for later use;

(3) after the dry yellow wine lees are subjected to microwave drying treatment, crushing the dry yellow wine lees into 80-mesh powder, weighing 100kg of the powder, placing the powder into a reaction kettle, adding 3000kg of purified water, soaking for 4 hours, fully and uniformly stirring to obtain uniform slurry, adjusting the pH to 5.0 by using a sulfuric acid solution, then adding 2170g of cellulase with the activity unit of 2000 mu per gram, 1550g of pectinase with the activity unit of 30000 mu per gram and 1550g of α -amylase with the activity unit of 6000 mu per gram into the slurry, uniformly stirring, heating to 55 ℃, carrying out heat preservation and enzymolysis for 24 hours, and slightly stirring for 10 minutes every 60 minutes during the period to obtain enzymolysis slurry;

(4) heating the slurry prepared in the step (3) to 100 ℃, boiling for 30min, naturally cooling, standing for 24h, filtering the slurry after standing to obtain 3022.5kg of filtrate, taking the filtrate for later use, and filtering the residue for later use;

(6) adding the liquid to be fermented in the step (5) into beer yeast in a weight ratio of: lactobacillus lactis: bacillus pumilus = 2: 1.5: 54.405kg of mixed bacteria in a proportion of 1.5, drilling a small hole in a barrel cover containing liquid to be fermented, inserting a plastic hose with the inner diameter of 1.5cm, screwing the barrel cover, inserting the other end of the plastic hose into a water barrel containing clean water to facilitate the discharge of gas in the fermentation liquid, and fermenting for one week at the temperature of 28-30 ℃ to obtain the fermentation liquid for later use;

(8) and (3) concentrating the concentrated solution with the specific gravity of 2.5-5.25 in the step (7) according to the following concentrated solution: the beer yeast powder weight ratio = 1: 4, uniformly mixing, drying in vacuum at the temperature of 31 ℃, granulating by using a 12-mesh sieve, drying in vacuum at the temperature of 30 ℃ until the water content is less than 7.0 percent, and sieving by using a 16-mesh sieve to obtain the feed additive rich in the active yeast for the animals;

(9) continuously concentrating the concentrated solution with the specific weight of 2.25-5.25 in the step (7) into a dry extract, wherein the dry extract comprises the following components in parts by weight: starch: complex microorganism = 1: 4: 1, wherein the composite microbial bacteria are prepared from beer yeast dry powder containing 200 hundred million per gram, lactobacillus thermophilus dry powder containing 200 hundred million per gram and bacillus subtilis dry powder containing 200 hundred million per gram according to the weight ratio of 2: 1: 1, granulating by using a 12-mesh sieve, drying by cold air, and sieving by using a 16-mesh sieve to obtain the solid microbial feed additive for animals;

(11) taking 35kg of filter residue mixed material obtained in the step (10), mixing the filter residue mixed material with 43kg of dried sheep manure and 10kg of bean pulp, and then adding 200 hundred million of streptococcus thermophilus dry powder containing viable bacteria per gram, 200 hundred million of bacillus licheniformis dry powder containing viable bacteria per gram and 200 hundred million of candida utilis dry powder containing viable bacteria per gram according to the weight ratio of 1: 1: 12kg of composite bacteria starter mixed according to the proportion of 1, uniformly mixing, adding a proper amount of water to adjust the water content to 40-45 percent, and obtaining a solid culture base material;

(12) putting the solid culture base material prepared in the step (11) into a fermentation tank for fermentation for 42 days, turning the fermentation tank in time when the fermentation temperature rises to 40 ℃, avoiding overhigh temperature, pouring the fermented material out of the fermentation tank after the fermentation is finished, airing, crushing, and sieving by a 55-mesh sieve to obtain fermented material powder;

(13) taking 85kg of the fermented material powder obtained in the step (12), mixing with sodium humate, potassium humate and ammonium humate according to the weight ratio of 1: 1.5: and 2, mixing 15kg of the composite humate obtained by mixing according to the weight ratio of 15kg uniformly, and then carrying out batching, granulation, screening and packaging according to a conventional method to obtain the bio-organic fertilizer product.

Example 3

(1) selecting dried ivy mosses, folium artemisiae argyi and schizonepeta leaves of plant leaves, respectively drying, and mixing the dried ivy mosses, the folium artemisiae argyi and the schizonepeta leaves according to a weight ratio of 10: 0.2: crushing the powder into 80-mesh powder according to a proportion of 0.5, weighing 100kg of the powder, placing the powder into a reaction kettle, adding 3000kg of purified water, soaking the powder for 6 hours at normal temperature, fully stirring to obtain uniform slurry, adjusting the pH of the slurry to 4.8 by using a sulfuric acid solution, then adding 2480g of cellulase with the activity unit of 2000 mu/g and 4650g of pectinase with the activity unit of 20000 mu/g into the slurry, uniformly stirring, heating to 48 ℃, carrying out heat preservation and enzymolysis for 3.6 hours, and slightly stirring for 5 minutes every 60 minutes to obtain enzymolysis slurry;

(2) heating the slurry prepared in the step (1) to 100 ℃, boiling for 30min, naturally cooling, standing for 18h, filtering the slurry after standing to obtain 3007kg of filtrate, taking the filtrate for later use, and filtering the residue for later use;

(3) after microwave drying treatment is carried out on dry brewer grains, the dry brewer grains are crushed into 80-mesh powder, 100kg of powder is weighed and placed into a reaction kettle, 3000kg of purified water is added, after soaking for 4 hours, the powder is fully and uniformly stirred to form uniform slurry, the pH value is adjusted to be 5.0 by using sulfuric acid solution, then 2170g of cellulase with the activity unit of 2000 mu m per gram, 1550g of pectinase with the activity unit of 30000 mu m per gram and 1550g of α -amylase with the activity unit of 6000 mu m per gram are added into the slurry, the mixture is uniformly stirred and heated to 58 ℃, heat preservation enzymolysis is carried out for 28 hours, and the mixture is gently stirred for 10 minutes every 60 minutes during the period, so that enzymolysis slurry is prepared;

(6) adding the liquid to be fermented in the step (5) into beer yeast in a weight ratio of: lactobacillus lactis: bacillus subtilis = 2: 1.5: 72.35kg of mixed bacteria according to the proportion of 1.5, drilling a small hole in a barrel cover containing the liquid to be fermented, inserting a plastic hose with the inner diameter of 1.5cm, screwing the barrel cover, inserting the other end of the plastic hose into a water barrel containing clean water, facilitating the discharge of gas in the fermented liquid, and fermenting for one week at the temperature of 28-30 ℃ to obtain the fermented liquid for later use;

(8) and (3) concentrating the concentrated solution with the specific gravity of 2.5-5.25 in the step (7) according to the following concentrated solution: the weight ratio of the candida powder is = 1: 4, uniformly mixing, drying in vacuum at the temperature of 31 ℃, granulating by using a 12-mesh sieve, drying in vacuum at the temperature of 30 ℃ until the water content is less than 7.0 percent, and sieving by using a 16-mesh sieve to obtain the feed additive rich in the active yeast for the animals;

(9) continuously concentrating the concentrated solution with the specific weight of 2.25-5.25 in the step (7) into a dry extract, wherein the dry extract comprises the following components in parts by weight: starch: complex microorganism = 1: 4: 1, wherein the composite microbial bacteria comprise beer yeast dry powder with 200 hundred million per gram, lactobacillus thermophilus dry powder with 200 hundred million per gram and bacillus firmus dry powder with 200 hundred million per gram according to the weight ratio of 2: 1: 1, granulating by using a 12-mesh sieve, drying by cold air, and sieving by using a 16-mesh sieve to obtain the solid microbial feed additive for animals;

(11) taking 30kg of filter residue mixed material obtained in the step (10), mixing the filter residue mixed material with 47kg of dried sheep manure and rabbit manure mixed manure and 10kg of peanut meal, and then adding a mixture of 200 hundred million of streptococcus thermophilus dry powder containing viable bacteria per gram, 200 hundred million of bacillus licheniformis dry powder containing viable bacteria per gram and 200 hundred million of candida utilis dry powder containing viable bacteria per gram according to a weight ratio of 1: 1: 13kg of composite bacteria starter mixed according to the proportion of 1, uniformly mixing, adding a proper amount of water to adjust the water content to 40-45 percent, and obtaining a solid culture base material;

(12) putting the solid culture base material prepared in the step (11) into a fermentation tank for fermentation for 43 days, turning the fermentation tank in time when the fermentation temperature rises to 40 ℃, avoiding overhigh temperature, pouring the fermented material out of the fermentation tank after the fermentation is finished, airing, crushing, and sieving by a 50-mesh sieve to obtain fermented material powder;

Example 4

(1) selecting soybean leaves, perilla leaves and peony leaves of plant leaves, respectively drying, and mixing the soybean leaves, the perilla leaves and the peony leaves according to a weight ratio of 10: 1: 8, crushing into 80-mesh powder, weighing 100kg of powder, placing into a reaction kettle, adding 3000kg of purified water, soaking at normal temperature for 6 hours, fully stirring to obtain uniform slurry, adjusting the pH of the slurry to 5.0 by using a sulfuric acid solution, then adding 2481g of cellulase with the activity unit of 2000 mu per gram and 5580g of pectinase with the activity unit of 20000 mu per gram into the slurry, uniformly stirring, heating to 48 ℃, carrying out heat preservation and enzymolysis for 4 hours, and slightly stirring for 5 minutes every 60 minutes to obtain enzymolysis slurry;

(2) heating the slurry prepared in the step (1) to 100 ℃, boiling for 30min, naturally cooling, standing for 18h, filtering the standing slurry to obtain 3034.9kg of filtrate, taking the filtrate for later use, and filtering the residue for later use;

(3) after microwave drying treatment is carried out on the barley dry alcohol vinasse, the barley dry alcohol vinasse is crushed into 80-mesh powder, 100kg of the powder is weighed and placed into a reaction kettle, 3000kg of purified water is added, after soaking for 4 hours, the barley dry alcohol vinasse is fully and uniformly stirred to form uniform slurry, the pH value is adjusted to be 5.0 by using a sulfuric acid solution, then 2325g of cellulase with the activity unit of 2000 mu m per gram, 775g of pectinase with the activity unit of 30000 mu m per gram and 1860g of α -amylase with the activity unit of 6000 mu m per gram are added into the slurry, the mixture is uniformly stirred, heated to 60 ℃, subjected to heat preservation and enzymolysis for 24 hours, and is gently stirred for 10 minutes every 60 minutes to;

(4) heating the slurry prepared in the step (3) to 100 ℃, boiling for 30min, naturally cooling, standing for 24h, filtering the slurry after standing to obtain 3025.5kg of filtrate, taking the filtrate for later use, and filtering the residue for later use;

(6) adding the fermentation solution to be fermented in the step (5) into feed yeast in a weight ratio of: lactobacillus lactis: bacillus pumilus = 2: 1.5: 60.60kg of mixed bacteria in a proportion of 1.5, drilling a small hole in a barrel cover containing liquid to be fermented, inserting a plastic hose with the inner diameter of 1.5cm, screwing the barrel cover, inserting the other end of the plastic hose into a water barrel containing clean water to facilitate the discharge of gas in the fermentation liquid, and fermenting for one week at the temperature of 28-30 ℃ to obtain the fermentation liquid for later use;

(9) continuously concentrating the concentrated solution with the specific weight of 2.25-5.25 in the step (7) into a dry extract, wherein the dry extract comprises the following components in parts by weight: starch: complex microorganism = 1: 4: 1, wherein the composite microorganism bacteria comprise 200 hundred million candida utilis dry powder per gram, 200 hundred million lactococcus lactis dry powder per gram and 200 hundred million bacillus subtilis dry powder per gram according to the weight ratio of 2: 1: 1, granulating by using a 12-mesh sieve, drying by cold air, and sieving by using a 16-mesh sieve to obtain the solid microbial feed additive for animals;

(11) taking 30kg of filter residue mixed material obtained in the step (10), mixing the filter residue mixed material with 42kg of dried cow dung and sheep dung mixed manure and 13kg of corn germ meal, and then adding 1 weight percent of lactobacillus reuteri dry powder with 200 hundred million viable bacteria concentration per gram, bacillus lentus dry powder with 200 hundred million viable bacteria concentration per gram and candida tropicalis dry powder with 200 hundred million viable bacteria concentration per gram: 1: 15kg of composite bacteria starter mixed according to the proportion of 1, uniformly mixing, adding a proper amount of water to adjust the water content to 40-45% to obtain a solid culture base material;

(12) putting the solid culture base material prepared in the step (11) into a fermentation tank for fermentation for 42 days, turning the fermentation tank in time when the fermentation temperature rises to 40 ℃, avoiding overhigh temperature, pouring the fermented material out of the fermentation tank after the fermentation is finished, airing, crushing, and sieving by a 60-mesh sieve to obtain fermented material powder;

(13) taking 90kg of the fermented material powder obtained in the step (12), mixing with sodium humate, potassium humate and ammonium humate according to the weight ratio of 1: 1.5: and 2, mixing 10kg of the composite humate obtained by mixing according to the weight ratio of 10kg uniformly, and then carrying out batching, granulation, screening and packaging according to a conventional method to obtain the bio-organic fertilizer product.

Example 5

(1) selecting and drying jujube leaves, folium kadsurae chinensis, pine needles, kudzu root leaves and salvia miltiorrhiza leaves of plant leaves respectively, wherein the weight ratio of the jujube leaves, the folium kadsurae chinensis, the pine needles, the kudzu root leaves and the salvia miltiorrhiza leaves is 10: 10: 2: 2: 1 proportion, crushing into 80-mesh powder, weighing 100kg of powder, placing into a reaction kettle, adding 3000kg of purified water, soaking at normal temperature for 6 hours, fully stirring to obtain uniform slurry, adjusting the pH of the slurry to 4.8 by using a sulfuric acid solution, then adding 2945g of cellulase with the activity unit of 2000 mu per gram and 7750g of pectinase with the activity unit of 20000 mu per gram into the slurry, uniformly stirring, heating to 50 ℃, preserving heat for enzymolysis for 4 hours, and slightly stirring for 5 minutes every 60 minutes to obtain enzymolysis slurry;

(3) after the corn wet alcohol grains are subjected to hot air drying treatment by an electrothermal drying box, the corn wet alcohol grains are crushed into 80-mesh powder, 100kg of the powder is weighed and placed into a reaction kettle, 3000kg of purified water is added, after the corn wet alcohol grains are soaked for 4 hours, the mixture is fully and uniformly stirred to form uniform slurry, the pH value is adjusted to 5.0 by using sulfuric acid solution, then 1860g of cellulase with the activity unit of 2000 mu per gram, 930g of pectinase with the activity unit of 30000 mu per gram and 1860g of α -amylase with the activity unit of 6000 mu per gram are added into the slurry, the mixture is uniformly stirred, the mixture is heated to 52 ℃, the mixture is subjected to heat preservation and enzymolysis for 30 hours, and the mixture is gently stirred for 10 minutes every 60;

(4) heating the slurry prepared in the step (3) to 100 ℃, boiling for 30min, naturally cooling, standing for 24h, filtering the slurry after standing to obtain 3025.6kg of filtrate, taking the filtrate for later use, and filtering the residue for later use;

(6) adding the liquid to be fermented in the step (5) into the mixture of red yeast: lactobacillus bulgaricus: bacillus coagulans = 2: 1.5: 66.53kg of mixed bacteria in a proportion of 1.5, drilling a small hole in a barrel cover containing liquid to be fermented, inserting a plastic hose with the inner diameter of 1.5cm, screwing the barrel cover, inserting the other end of the plastic hose into a water barrel containing clean water to facilitate the discharge of gas in the fermentation liquid, and fermenting for one week at the temperature of 28-30 ℃ to obtain the fermentation liquid for later use;

(9) continuously concentrating the concentrated solution with the specific weight of 2.25-5.25 in the step (7) into a dry extract, wherein the dry extract comprises the following components in parts by weight: starch: complex microorganism = 1: 4: 1, wherein the composite microbial bacteria are prepared from feed yeast dry powder with 200 hundred million per gram, lactobacillus plantarum dry powder with 200 hundred million per gram and bacillus subtilis dry powder with 200 hundred million per gram according to the weight ratio of 2: 1: 1, granulating by using a 12-mesh sieve, drying by cold air, and sieving by using a 16-mesh sieve to obtain the solid microbial feed additive for animals;

(11) taking 27kg of filter residue mixed material obtained in the step (10), mixing the filter residue mixed material with 50kg of dried pig manure and cow manure mixed manure and 13kg of cottonseed meal, and then adding the mixture into a mixer, wherein the mixer is prepared by mixing, by weight, 200 hundred million of streptococcus lactis dry powder containing viable bacteria per gram, 200 hundred million of bacillus circulans dry powder containing viable bacteria per gram and 200 hundred million of brewer's yeast dry powder containing viable bacteria per gram, and the mixer is characterized in that: 1: 10kg of composite bacteria starter mixed according to the proportion of 1, uniformly mixing, adding a proper amount of water to adjust the water content to 40-45% to obtain a solid culture base material;

(12) putting the solid culture base material prepared in the step (11) into a fermentation tank for fermentation for 45 days, turning the fermentation tank in time when the fermentation temperature rises to 40 ℃, avoiding overhigh temperature, pouring the fermented material out of the fermentation tank after the fermentation is finished, airing, crushing, and sieving by a 55-mesh sieve to obtain fermented material powder;

(13) taking 84kg of the fermented material powder obtained in the step (12), mixing with sodium humate, potassium humate and ammonium humate according to the weight ratio of 1: 1.5: and mixing 16kg of the compound humate obtained by mixing 2 weight ratios uniformly, and then carrying out batching, granulation, screening and packaging according to a conventional method to obtain the bio-organic fertilizer product.

Example 6

(1) picking and drying wild chrysanthemum leaf, ginkgo leaf, aloe leaf and houttuynia cordata leaf of plant leaf, respectively, wherein the weight ratio of the wild chrysanthemum leaf to the ginkgo leaf to the aloe leaf to the houttuynia cordata leaf is 10: 0.5: 0.5: 1 proportion, crushing into 80-mesh powder, weighing 100kg of powder, placing into a reaction kettle, adding 3000kg of purified water, soaking at normal temperature for 6 hours, fully stirring to obtain uniform slurry, adjusting the pH of the slurry to 4.6 by using a sulfuric acid solution, then adding 2325g of cellulase with the activity unit of 2000 mu m per gram and 3875g of pectinase with the activity unit of 20000 mu m per gram into the slurry, uniformly stirring, heating to 50 ℃, preserving heat, performing enzymolysis for 3.5 hours, and slightly stirring for 5 minutes every 60 minutes to obtain enzymolysis slurry;

(3) after microwave drying treatment is carried out on a mixture of corn dry alcohol vinasse soluble substances and barley dry alcohol vinasse containing the soluble substances according to a weight ratio of 1: 1, the mixture is crushed into 80-mesh powder, 100kg of the powder is weighed and placed into a reaction kettle, 3000kg of purified water is added, after soaking for 4 hours, the mixture is fully stirred uniformly to form uniform slurry, the pH value is adjusted to be 5.0 by using a sulfuric acid solution, 1705g of cellulase with the activity unit of 2000 mu per gram, 930g of pectinase with the activity unit of 30000 mu per gram and 1550g of α -amylase with the activity unit of 6000 mu per gram are added into the slurry, the mixture is stirred uniformly, the mixture is heated to 60 ℃, heat preservation enzymolysis is carried out for 24 hours, and during the period, the mixture is stirred slightly for 10 minutes every 60 minutes to prepare enzymolysis;

(6) adding the liquid to be fermented in the step (5) into beer yeast in a weight ratio of: lactobacillus plantarum: bacillus subtilis = 2: 1.5: 54.55kg of mixed bacteria according to the proportion of 1.5, drilling a small hole in a barrel cover containing the liquid to be fermented, inserting a plastic hose with the inner diameter of 1.5cm, screwing the barrel cover, inserting the other end of the plastic hose into a water barrel containing clean water, facilitating the discharge of gas in the fermented liquid, and fermenting for one week at the temperature of 28-30 ℃ to obtain the fermented liquid for later use;

(11) taking 30kg of the filter residue mixed material obtained in the step (10), mixing the filter residue mixed material with 48kg of dried cow dung and rabbit dung mixed manure and 11kg of sunflower seed kernel meal, and then adding 1 weight percent of lactobacillus delbrueckii dry powder with 200 hundred million viable bacteria concentration per gram, 200 hundred million bacillus licheniformis dry powder with 200 hundred million viable bacteria concentration per gram and 200 hundred million candida tropicalis dry powder with 200 hundred million viable bacteria concentration per gram: 1: 1, mixing 11kg of composite bacteria starter in proportion, uniformly mixing, adding a proper amount of water to adjust the water content to 40-45% to obtain a solid culture base material;

(12) putting the solid culture base material prepared in the step (11) into a fermentation tank for fermentation for 43 days, turning the fermentation tank in time when the fermentation temperature rises to 40 ℃, avoiding overhigh temperature, pouring the fermented material out of the fermentation tank after the fermentation is finished, airing, crushing, and sieving by a 60-mesh sieve to obtain fermented material powder;

(13) taking 88kg of the fermented material powder obtained in the step (12), mixing with sodium humate, potassium humate and ammonium humate according to the weight ratio of 1: 1.5: and after 12kg of the composite humate which is mixed according to the weight ratio of 2 is uniformly mixed, the mixture is subjected to batching, granulation, screening and packaging according to a conventional method, and the bio-organic fertilizer product is obtained.

Example 7

(1) picking and drying rhizoma polygonati leaves, leonurus leaves, folium isatidis, ligusticum wallichii leaves and poplar leaves of plant leaves respectively according to the weight ratio of the rhizoma polygonati leaves, the leonurus leaves, the folium isatidis, the ligusticum wallichii leaves and the poplar leaves of 5: 1: 1: 0.5: crushing the materials into powder of 80 meshes according to a proportion of 10, weighing 100kg of the powder, placing the powder into a reaction kettle, adding 3000kg of purified water, soaking the powder for 6 hours at normal temperature, fully stirring to obtain uniform slurry, adjusting the pH of the slurry to 4.6 by using a sulfuric acid solution, then adding 2790g of cellulase with the activity unit of 2000 mu/g and 6820g of pectinase with the activity unit of 20000 mu/g into the slurry, uniformly stirring, heating to 50 ℃, preserving heat, performing enzymolysis for 4 hours, and slightly stirring for 5 minutes every 60 minutes to obtain enzymolysis slurry;

(3) after a mixture of corn wet alcohol grains and barley wet alcohol grains according to a weight ratio of 1: 1 is subjected to hot air drying treatment by an electric heating oven, the mixture is crushed into 80-mesh powder, 100kg of the powder is weighed and placed into a reaction kettle, 3000kg of purified water is added, after the mixture is soaked for 4 hours, the mixture is fully and uniformly stirred to form uniform slurry, the pH value is adjusted to 5.0 by using a sulfuric acid solution, then cellulase 2015g with an activity unit of 2000 mu per gram, pectinase 1085g with an activity unit of 30000 mu per gram and α -amylase 1705g with an activity unit of 6000 mu per gram are added into the slurry, the mixture is uniformly stirred and heated to 55 ℃, the temperature is kept for enzymolysis for 26 hours, and the mixture is gently stirred for 10 minutes every 60 minutes during enzymolysis to obtain enzymolysis slurry;

(6) adding the fermentation solution to be fermented in the step (5) into feed yeast in a weight ratio of: lactic acid streptococcus: bacillus licheniformis = 2: 1.5: mixing bacteria 72.73kg according to the proportion of 1.5, drilling a small hole in a barrel cover containing the liquid to be fermented, inserting a plastic hose with the inner diameter of 1.5cm, screwing the barrel cover, inserting the other end of the plastic hose into a water barrel containing clean water, facilitating the discharge of gas in the fermentation liquid, and fermenting for one week at the temperature of 28-30 ℃ to obtain the fermentation liquid for later use;

(9) continuously concentrating the concentrated solution with the specific weight of 2.25-5.25 in the step (7) into a dry extract, wherein the dry extract comprises the following components in parts by weight: starch: complex microorganism = 1: 4: 1, wherein the composite microorganism bacteria comprise beer yeast dry powder with 200 hundred million per gram, lactobacillus lactis dry powder with 200 hundred million per gram and bacillus lentus dry powder with 200 hundred million per gram according to the weight ratio of 2: 1: 1, granulating by using a 12-mesh sieve, drying by cold air, and sieving by using a 16-mesh sieve to obtain the solid microbial feed additive for animals;

(11) taking 32kg of the filter residue mixed material obtained in the step (10), mixing the filter residue mixed material with 46kg of dried cow dung, bean pulp and safflower seed meal according to the weight ratio of 1: 1, adding 9kg of streptococcus thermophilus dry powder with viable bacteria concentration of 200 hundred million per gram, bacillus licheniformis dry powder with viable bacteria concentration of 200 hundred million per gram and candida utilis dry powder with viable bacteria concentration of 200 hundred million per gram according to the weight ratio of 1: 1: 13kg of composite bacteria starter mixed according to the proportion of 1, uniformly mixing, adding a proper amount of water to adjust the water content to 40-45 percent, and obtaining a solid culture base material;

(12) putting the solid culture base material prepared in the step (11) into a fermentation tank for fermentation for 40 days, turning the fermentation tank in time when the fermentation temperature rises to 40 ℃, avoiding overhigh temperature, pouring the fermented material out of the fermentation tank after the fermentation is finished, airing, crushing, and sieving by a 55-mesh sieve to obtain fermented material powder;

Example 8

(1) picking and drying potato leaves, sweet wormwood leaves, maidenhair leaves, poplar leaves and cockscomb leaves of plant leaves respectively, wherein the weight ratio of the potato leaves to the sweet wormwood leaves to the maidenhair leaves to the poplar leaves to the cockscomb leaves is 10: 0.5: 1: 8: crushing the powder into 80-mesh powder according to a proportion of 0.5, weighing 100kg of the powder, placing the powder into a reaction kettle, adding 3000kg of purified water, soaking the powder for 6 hours at normal temperature, fully stirring to obtain uniform slurry, adjusting the pH of the slurry to 4.7 by using a sulfuric acid solution, then adding 2945g of cellulase with the activity unit of 2000 mu m per gram and 7750g of pectinase with the activity unit of 20000 mu m per gram into the slurry, uniformly stirring, heating to 50 ℃, carrying out heat preservation and enzymolysis for 4.5 hours, and slightly stirring for 5 minutes every 60 minutes to obtain enzymolysis slurry;

(3) after a mixture of dry alcohol lees soluble substances and wet alcohol lees soluble substances in a weight ratio of 1: 2 is subjected to microwave drying treatment, the mixture is crushed into 80-mesh powder, 100kg of the powder is weighed and placed into a reaction kettle, 3000kg of purified water is added, after the mixture is soaked for 4 hours, the mixture is fully and uniformly stirred to form uniform slurry, the pH value is adjusted to 5.0 by using a sulfuric acid solution, 1705g of cellulase with an activity unit of 2000 mu per gram, 775g of pectinase with an activity unit of 30000 mu per gram and 1705g of α -amylase with an activity unit of 6000 mu per gram are added into the slurry, the mixture is stirred uniformly, the mixture is heated to 53 ℃, the mixture is subjected to heat preservation and enzymolysis for 28 hours, and is gently stirred for 10 minutes every 60 minutes during the enzymolysis, so that;

(4) heating the slurry prepared in the step (3) to 100 ℃, boiling for 30min, naturally cooling, standing for 24h, filtering the slurry after standing to obtain 3031.8kg of filtrate, taking the filtrate for later use, and filtering the residue for later use;

(6) adding the liquid to be fermented in the step (5) into beer yeast in a weight ratio of: lactobacillus plantarum: bacillus subtilis = 2: 1.5: 72.65kg of mixed bacteria in a proportion of 1.5, drilling a small hole in a barrel cover containing liquid to be fermented, inserting a plastic hose with the inner diameter of 1.5cm, screwing the barrel cover, inserting the other end of the plastic hose into a water barrel containing clean water to facilitate the discharge of gas in the fermentation liquid, and fermenting for one week at the temperature of 28-30 ℃ to obtain the fermentation liquid for later use;

(9) continuously concentrating the concentrated solution with the specific weight of 2.25-5.25 in the step (7) into a dry extract, wherein the dry extract comprises the following components in parts by weight: starch: complex microorganism = 1: 4: 1, wherein the composite microbial bacteria are prepared from beer yeast dry powder containing 200 hundred million per gram, lactobacillus thermophilus dry powder containing 200 hundred million per gram and bacillus stearothermophilus dry powder containing 200 hundred million per gram according to the weight ratio of 2: 1: 1, granulating by using a 12-mesh sieve, drying by cold air, and sieving by using a 16-mesh sieve to obtain the solid microbial feed additive for animals;

(11) taking 29kg of the filter residue mixed material obtained in the step (10), mixing the filter residue mixed material with 52kg of dried sheep manure, cottonseed meal and pecan meal in a weight ratio of 1: 1, adding 9kg of lactobacillus acidophilus dry powder with viable bacteria concentration of 200 hundred million per gram, bacillus licheniformis dry powder with viable bacteria concentration of 200 hundred million per gram and candida utilis dry powder with viable bacteria concentration of 200 hundred million per gram according to the weight ratio of 1: 1: 10kg of composite bacteria starter mixed according to the proportion of 1, uniformly mixing, adding a proper amount of water to adjust the water content to 40-45% to obtain a solid culture base material;

(12) putting the solid culture base material prepared in the step (11) into a fermentation tank for fermentation for 40 days, turning the fermentation tank in time when the fermentation temperature rises to 40 ℃, avoiding overhigh temperature, pouring the fermented material out of the fermentation tank after the fermentation is finished, airing, crushing, and sieving by a 50-mesh sieve to obtain fermented material powder;

(13) mixing 89kg of the fermented material powder obtained in the step (12) with sodium humate, potassium humate and ammonium humate according to the weight ratio of 1: 1.5: and (2) mixing 11kg of the composite humate obtained by mixing according to the weight ratio of 2 uniformly, and then carrying out batching, granulation, screening and packaging according to a conventional method to obtain the bio-organic fertilizer product.

Example 9

(1) picking and drying the pecan leaves, the bletilla striata leaves, the cyrtomium fortunei leaves, the rhizoma atractylodis leaves and the magnolia officinalis leaves of the plant leaves respectively, wherein the weight ratio of the pecan leaves, the bletilla striata leaves, the cyrtomium fortunei leaves, the rhizoma atractylodis leaves and the magnolia officinalis leaves is 10: 1: 1: 1: 2, crushing into 80-mesh powder, weighing 100kg of powder, placing into a reaction kettle, adding 3000kg of purified water, soaking at normal temperature for 6 hours, fully stirring to obtain uniform slurry, adjusting the pH of the slurry to 4.8 by using a sulfuric acid solution, then adding 2635g of cellulase with the activity unit of 2000 mu per gram and 6820g of pectinase with the activity unit of 20000 mu per gram into the slurry, stirring uniformly, heating to 46 ℃, performing heat preservation and enzymolysis for 4.3 hours, and slightly stirring for 5 minutes every 60 minutes to obtain enzymolysis slurry;

(2) heating the slurry prepared in the step (1) to 100 ℃, boiling for 30min, naturally cooling, standing for 18h, filtering the standing slurry to obtain 3031.8kg of filtrate, taking the filtrate for later use, and filtering the residue for later use;

(3) drying a mixture of dry grain alcohol vinasse and wet grain alcohol vinasse soluble substances according to a weight ratio of 1: 2 by microwave, crushing the mixture into 80-mesh powder, weighing 100kg of the powder, placing the powder into a reaction kettle, adding 3000kg of purified water, soaking for 4h, fully and uniformly stirring to obtain uniform slurry, adjusting the pH to 5.0 by using a sulfuric acid solution, adding 2015g of cellulase with an activity unit of 2000 mu per gram, 1085g of pectinase with an activity unit of 30000 mu per gram and α -amylase 1395g with an activity unit of 6000 mu per gram into the slurry, uniformly stirring, heating to 50 ℃, carrying out heat preservation and enzymolysis for 30h, and slightly stirring for 10min every 60min during the period to obtain enzymolysis slurry;

(6) adding the liquid to be fermented in the step (5) into the mixture of red yeast: lactobacillus bulgaricus: bacillus licheniformis = 2: 1.5: 60.57kg of mixed bacteria in a proportion of 1.5, drilling a small hole in a barrel cover containing liquid to be fermented, inserting a plastic hose with the inner diameter of 1.5cm, screwing the barrel cover, inserting the other end of the plastic hose into a water barrel containing clean water to facilitate the discharge of gas in the fermentation liquid, and fermenting for one week at the temperature of 28-30 ℃ to obtain the fermentation liquid for later use;

(9) continuously concentrating the concentrated solution with the specific weight of 2.25-5.25 in the step (7) into a dry extract, wherein the dry extract comprises the following components in parts by weight: starch: complex microorganism = 1: 4: 1, wherein the composite microbial bacteria are prepared from beer yeast dry powder containing 200 hundred million per gram, lactococcus lactis dry powder containing 200 hundred million per gram and bacillus subtilis dry powder containing 200 hundred million per gram according to the weight ratio of 2: 1: 1, granulating by using a 12-mesh sieve, drying by cold air, and sieving by using a 16-mesh sieve to obtain the solid microbial feed additive for animals;

(11) taking 26kg of filter residue mixed material obtained in the step (10), 50kg of dried rabbit manure and cow manure mixed manure, and wheat germ meal and corn germ meal in a weight ratio of 1: 1, adding lactobacillus acidophilus dry powder with the viable bacteria concentration of 200 hundred million per gram, bacillus licheniformis dry powder with the viable bacteria concentration of 200 hundred million per gram and candida tropicalis dry powder with the viable bacteria concentration of 200 hundred million per gram according to the weight ratio of 1: 1: 12kg of composite bacteria starter mixed according to the proportion of 1, uniformly mixing, adding a proper amount of water to adjust the water content to 40-45 percent, and obtaining a solid culture base material;

(13) taking 83kg of the fermented material powder obtained in the step (12), mixing with sodium humate, potassium humate and ammonium humate according to the weight ratio of 1: 1.5: and mixing 17kg of the composite humate obtained by mixing the components in a weight ratio of 2 uniformly, and then carrying out batching, granulation, screening and packaging according to a conventional method to obtain the bio-organic fertilizer product.

Example 10

(1) selecting and selecting sunflower leaves, paulownia leaves, acanthopanax leaves and angelica dahurica leaves of plant leaves, respectively drying, and mixing the sunflower leaves, the paulownia leaves, the acanthopanax leaves and the angelica dahurica leaves according to a weight ratio of 5: 10: 2: 1 proportion, crushing into 80-mesh powder, weighing 100kg of powder, placing into a reaction kettle, adding 3000kg of purified water, soaking at normal temperature for 6 hours, fully stirring to obtain uniform slurry, adjusting the pH of the slurry to 5.0 by using a sulfuric acid solution, then adding 2945g of cellulase with the activity unit of 2000 mu per gram and 7440g of pectinase with the activity unit of 20000 mu per gram into the slurry, uniformly stirring, heating to 47 ℃, performing heat preservation and enzymolysis for 4.5 hours, and slightly stirring for 5 minutes every 60 minutes to obtain enzymolysis slurry;

(3) after microwave drying treatment is carried out on the soluble-containing grain dry alcohol vinasse, the grain dry alcohol vinasse is crushed into 80-mesh powder, 100kg of the powder is weighed and placed into a reaction kettle, 3000kg of purified water is added, after soaking for 4 hours, the mixture is fully and uniformly stirred to form uniform slurry, 1705g of sulfuric acid solution, 775g of pectinase with the activity unit of 30000 mu m per gram and 1395g of α -amylase with the activity unit of 6000 mu m per gram are used for uniformly stirring, the mixture is heated to 58 ℃, heat preservation and enzymolysis are carried out for 26 hours, and slight stirring is carried out for 10 minutes every 60 minutes during the period, so that enzymolysis slurry is prepared;

(6) adding the fermentation solution to be fermented in the step (5) into feed yeast in a weight ratio of: lactobacillus bulgaricus: bacillus subtilis = 2: 1.5: 54.489kg of mixed bacteria in a proportion of 1.5, drilling a small hole in a barrel cover containing liquid to be fermented, inserting a plastic hose with the inner diameter of 1.5cm, screwing the barrel cover, inserting the other end of the plastic hose into a water barrel containing clean water to facilitate the discharge of gas in the fermentation liquid, and fermenting for one week at the temperature of 28-30 ℃ to obtain the fermentation liquid for later use;

(9) continuously concentrating the concentrated solution with the specific weight of 2.25-5.25 in the step (7) into a dry extract, wherein the dry extract comprises the following components in parts by weight: starch: complex microorganism = 1: 4: 1, wherein the composite microorganism bacteria comprise 200 hundred million candida utilis dry powder per gram, 200 hundred million lactobacillus plantarum dry powder per gram and 200 hundred million bacillus firmus dry powder per gram according to the weight ratio of 2: 1: 1, granulating by using a 12-mesh sieve, drying by cold air, and sieving by using a 16-mesh sieve to obtain the solid microbial feed additive for animals;

(11) taking 32kg of filter residue mixed material obtained in the step (10), 44kg of dried pig manure and rabbit manure mixed manure, and rapeseed meal and wild jujube meal according to a weight ratio of 8: 2, then adding lactobacillus acidophilus dry powder with the viable bacteria concentration of 200 hundred million per gram, bacillus subtilis dry powder with the viable bacteria concentration of 200 hundred million per gram and candida utilis dry powder with the viable bacteria concentration of 200 hundred million per gram according to the weight ratio of 1: 1: 12kg of composite bacteria starter mixed according to the proportion of 1, uniformly mixing, adding a proper amount of water to adjust the water content to 40-45 percent, and obtaining a solid culture base material;

Example 11

(1) picking and selecting chrysanthemum leaves, barley leaves, safflower leaves and angelica leaves of plant leaves, respectively drying, and mixing the chrysanthemum leaves, the barley leaves, the safflower leaves and the angelica leaves according to the weight ratio of 10: 10: 1: 1, crushing into 80-mesh powder, weighing 100kg of powder, placing into a reaction kettle, adding 3000kg of purified water, soaking at normal temperature for 6 hours, fully stirring to obtain uniform slurry, adjusting the pH of the slurry to 4.5 by using a sulfuric acid solution, then adding 2480g of cellulase with the activity unit of 2000 mu per gram and 4030g of pectinase with the activity unit of 20000 mu into the slurry, stirring uniformly, heating to 46 ℃, performing heat preservation and enzymolysis for 4 hours, and slightly stirring for 5 minutes every 60 minutes to obtain enzymolysis slurry;

(2) heating the slurry prepared in the step (1) to 100 ℃, boiling for 30min, naturally cooling, standing for 18h, filtering the standing slurry to obtain 3025.6kg of filtrate, taking the filtrate for later use, and filtering the residue for later use;

(3) after microwave drying treatment is carried out on a mixture of sorghum dry alcohol vinasse soluble matters and rye dry alcohol vinasse containing the soluble matters according to a weight ratio of 1: 1, the mixture is crushed into 80-mesh powder, 100kg of the powder is weighed and placed into a reaction kettle, 3000kg of purified water is added, after soaking for 4 hours, the mixture is fully stirred uniformly to form uniform slurry, the pH value is adjusted to be 5.0 by using a sulfuric acid solution, 2480g of cellulase with the activity unit of 2000 mu per gram, 775g of pectinase with the activity unit of 30000 mu per gram and 1550g of α -amylase with the activity unit of 6000 mu per gram are added into the slurry, the mixture is stirred uniformly, the mixture is heated to 52 ℃, the temperature is kept for enzymolysis for 28 hours, and the mixture is stirred slightly for 10 minutes every 60 minutes during enzymolysis, so as to obtain;

(6) adding the liquid to be fermented in the step (5) into beer yeast in a weight ratio of: lactobacillus plantarum: bacillus lentus = 2: 1.5: 54.46kg of mixed bacteria in a proportion of 1.5, drilling a small hole in a barrel cover containing liquid to be fermented, inserting a plastic hose with the inner diameter of 1.5cm, screwing the barrel cover, inserting the other end of the plastic hose into a water barrel containing clean water to facilitate the discharge of gas in the fermentation liquid, and fermenting for one week at the temperature of 28-30 ℃ to obtain the fermentation liquid for later use;

(9) continuously concentrating the concentrated solution with the specific weight of 2.25-5.25 in the step (7) into a dry extract, wherein the dry extract comprises the following components in parts by weight: starch: complex microorganism = 1: 4: 1, wherein the composite microorganism bacteria comprise 200 hundred million candida utilis dry powder per gram, 200 hundred million lactococcus lactis dry powder per gram and 200 hundred million bacillus coagulans dry powder per gram according to the weight ratio of 2: 1: 1, granulating by using a 12-mesh sieve, drying by cold air, and sieving by using a 16-mesh sieve to obtain the solid microbial feed additive for animals;

(11) taking 27kg of the filter residue mixed material obtained in the step (10), 50kg of dried sheep manure and pig manure mixed manure and peanut meal and rapeseed meal according to the weight ratio of 1: 1, adding lactobacillus acidophilus dry powder with the viable bacteria concentration of 200 hundred million per gram, bacillus licheniformis dry powder with the viable bacteria concentration of 200 hundred million per gram and brewing yeast dry powder with the viable bacteria concentration of 200 hundred million per gram according to the weight ratio of 1: 1: 15kg of composite bacteria starter mixed according to the proportion of 1, uniformly mixing, adding a proper amount of water to adjust the water content to 40-45% to obtain a solid culture base material;

(12) putting the solid culture base material prepared in the step (11) into a fermentation tank for fermentation for 40 days, turning the fermentation tank in time when the fermentation temperature rises to 40 ℃, avoiding overhigh temperature, pouring the fermented material out of the fermentation tank after the fermentation is finished, airing, crushing, and sieving by a 60-mesh sieve to obtain fermented material powder;

Example 12

(1) picking sweet potato leaves, chicory leaves and beet leaves of plant leaves, respectively drying, and mixing the sweet potato leaves, the chicory leaves and the beet leaves according to the weight ratio of 10: 1: crushing the raw materials into powder of 80 meshes according to a proportion of 6, weighing 100kg of the powder, placing the powder into a reaction kettle, adding 3000kg of purified water, soaking the raw materials for 6 hours at normal temperature, fully stirring to obtain uniform slurry, adjusting the pH of the slurry to 4.9 by using a sulfuric acid solution, then adding 2790g of cellulase with the activity unit of 2000 mu/g and 7130g of pectinase with the activity unit of 20000 mu/g into the slurry, stirring uniformly, heating to 48 ℃, performing heat preservation and enzymolysis for 4.2 hours, and slightly stirring for 5 minutes every 60 minutes to obtain enzymolysis slurry;

(3) after a mixture of dry white wine lees and dry beer lees in a weight ratio of 1: 1 is subjected to microwave drying treatment, the mixture is crushed into 80-mesh powder, 100kg of the powder is weighed and placed into a reaction kettle, 3000kg of purified water is added, after the mixture is soaked for 4 hours, the mixture is fully and uniformly stirred to form uniform slurry, the pH value is adjusted to 5.0 by using a sulfuric acid solution, then 2325g of cellulase with an activity unit of 2000 mu per gram, 1705g of pectinase with an activity unit of 30000 mu per gram and 1860g of α -amylase with an activity unit of 6000 mu per gram are added into the slurry, the mixture is uniformly stirred, the mixture is heated to 55 ℃, the mixture is subjected to heat preservation and enzymolysis for 29 hours, and is gently stirred for 10 minutes every 60 minutes during the enzymolysis, so;

(6) adding the liquid to be fermented in the step (5) into the mixture of red yeast: lactobacillus plantarum: bacillus subtilis = 2: 1.5: 72.43kg of mixed bacteria in a proportion of 1.5, drilling a small hole in a barrel cover containing liquid to be fermented, inserting a plastic hose with the inner diameter of 1.5cm, screwing the barrel cover, inserting the other end of the plastic hose into a water barrel containing clean water to facilitate the discharge of gas in the fermentation liquid, and fermenting for one week at the temperature of 28-30 ℃ to obtain the fermentation liquid for later use;

(11) taking 20kg of the filter residue mixed material obtained in the step (10), mixing the filter residue mixed material with 60kg of dried rabbit manure, sesame meal and rapeseed meal according to the weight ratio of 2: 8, adding 200 hundred million of lactobacillus delbrueckii dry powder with viable bacteria concentration per gram, 200 hundred million of bacillus licheniformis dry powder with viable bacteria concentration per gram and 200 hundred million of candida tropicalis dry powder with viable bacteria concentration per gram according to the weight ratio of 1: 1: 10kg of composite bacteria starter mixed according to the proportion of 1, uniformly mixing, adding a proper amount of water to adjust the water content to 40-45% to obtain a solid culture base material;

(12) putting the solid culture base material prepared in the step (11) into a fermentation tank for fermentation for 45 days, turning the fermentation tank in time when the fermentation temperature rises to 40 ℃, avoiding overhigh temperature, pouring the fermented material out of the fermentation tank after the fermentation is finished, airing, crushing, and sieving by a 60-mesh sieve to obtain fermented material powder;

(13) taking 87kg of the fermented material powder obtained in the step (12), mixing the fermented material powder with sodium humate, potassium humate and ammonium humate according to the weight ratio of 1: 1.5: and mixing 13kg of the compound humate obtained by mixing 2 weight ratios uniformly, and then carrying out batching, granulation, screening and packaging according to a conventional method to obtain the bio-organic fertilizer product.

Example 13

(1) selecting and selecting hawthorn leaves, microcos paniculata leaves, stevia rebaudiana leaves and okra leaves of plant leaves, respectively drying, and mixing the hawthorn leaves, microcos paniculata leaves, stevia rebaudiana leaves and okra leaves according to a weight ratio of 1: 1: 0.5: crushing the powder into 80-mesh powder according to a proportion of 0.5, weighing 100kg of the powder, placing the powder into a reaction kettle, adding 3000kg of purified water, soaking the powder for 6 hours at normal temperature, fully stirring to obtain uniform slurry, adjusting the pH of the slurry to 5.0 by using a sulfuric acid solution, then adding 2945g of cellulase with the activity unit of 2000 mu m per gram and 7750g of pectinase with the activity unit of 20000 mu m per gram into the slurry, uniformly stirring, heating to 50 ℃, carrying out heat preservation and enzymolysis for 4.5 hours, and slightly stirring for 5 minutes every 60 minutes to obtain enzymolysis slurry;

(3) after a mixture of dry yellow wine lees and dry potato alcohol lees in a weight ratio of 1: 1 is subjected to microwave drying treatment, the mixture is crushed into 80-mesh powder, 100kg of the powder is weighed and placed into a reaction kettle, 3000kg of purified water is added, after the mixture is soaked for 4 hours, the mixture is fully and uniformly stirred to form uniform slurry, the pH value is adjusted to 5.0 by using a sulfuric acid solution, 2170g of cellulase with an activity unit of 2000 mu per gram, 1395g of pectinase with an activity unit of 30000 mu per gram and 1705g of α -amylase with an activity unit of 6000 mu per gram are added into the slurry, the mixture is uniformly stirred, the mixture is heated to 55 ℃, the mixture is subjected to heat preservation and enzymolysis for 30 hours, and the mixture is lightly stirred for 10 minutes every 60 minutes during the enzymolysis, so;

(6) adding the liquid to be fermented in the step (5) into beer yeast in a weight ratio of: lactobacillus plantarum: bacillus subtilis = 2: 1.5: 72.54kg of mixed bacteria in a proportion of 1.5, drilling a small hole in a barrel cover containing liquid to be fermented, inserting a plastic hose with the inner diameter of 1.5cm, screwing the barrel cover, inserting the other end of the plastic hose into a water barrel containing clean water to facilitate the discharge of gas in the fermentation liquid, and fermenting for one week at the temperature of 28-30 ℃ to obtain the fermentation liquid for later use;

(11) taking 35kg of the filter residue mixed material obtained in the step (10), mixing the filter residue mixed material with 45kg of dried sheep manure, bean pulp and wheat germ pulp in a weight ratio of 1: 1, adding 8kg of streptococcus lactis dry powder with viable bacteria concentration of 200 hundred million per gram, bacillus licheniformis dry powder with viable bacteria concentration of 200 hundred million per gram and candida utilis dry powder with viable bacteria concentration of 200 hundred million per gram according to the weight ratio of 1: 1: 12kg of composite bacteria starter mixed according to the proportion of 1, uniformly mixing, adding a proper amount of water to adjust the water content to 40-45 percent, and obtaining a solid culture base material;

(13) taking 86kg of the fermented material powder obtained in the step (12), mixing with sodium humate, potassium humate and ammonium humate according to the weight ratio of 1: 1.5: and after 14kg of the compound humate which is mixed according to the weight ratio of 2 is uniformly mixed, the materials are proportioned, granulated, screened and packaged according to a conventional method, and the bio-organic fertilizer product is obtained.

Example 14

(1) picking and drying Chinese yam leaves, carrot leaves, garlic leaves, celery leaves and pepper leaves of plant leaves respectively, wherein the weight ratio of the Chinese yam leaves, the carrot leaves, the garlic leaves, the celery leaves and the pepper leaves is 10: 10: 1: 2: crushing the powder into 80-mesh powder according to a proportion of 0.5, weighing 100kg of the powder, placing the powder into a reaction kettle, adding 3000kg of purified water, soaking the powder for 6 hours at normal temperature, fully stirring to obtain uniform slurry, adjusting the pH of the slurry to 5.0 by using a sulfuric acid solution, then adding 2883g of cellulase with an activity unit of 2000 mu m per gram and 7130g of pectinase with an activity unit of 20000 mu m per gram into the slurry, uniformly stirring, heating to 50 ℃, carrying out heat preservation and enzymolysis for 4 hours, and slightly stirring for 5 minutes every 60 minutes to obtain enzymolysis slurry;

(3) after microwave drying treatment is carried out on a mixture of the rice dry alcohol vinasse and the wheat dry alcohol vinasse soluble substances according to a weight ratio of 1: 1, the mixture is crushed into 80-mesh powder, 100kg of the powder is weighed and placed into a reaction kettle, 3000kg of purified water is added, after soaking for 4 hours, the mixture is fully and uniformly stirred to form uniform slurry, the pH value is adjusted to 5.0 by using a sulfuric acid solution, 2170g of cellulase with an activity unit of 2000 mu per gram, 1395g of pectinase with an activity unit of 30000 mu per gram and 1705g of α -amylase with an activity unit of 6000 mu per gram are added into the slurry, the mixture is uniformly stirred, the mixture is heated to 53 ℃, heat preservation enzymolysis is carried out for 30 hours, and the mixture is lightly stirred for 10 minutes every 60 minutes during enzymolysis, so as;

(4) heating the slurry prepared in the step (3) to 100 ℃, boiling for 30min, naturally cooling, standing for 24h, filtering the slurry after standing to obtain 3016.3kg of filtrate, taking the filtrate for later use, and filtering the residue for later use;

(6) adding the fermentation solution to be fermented in the step (5) into feed yeast in a weight ratio of: lactobacillus lactis: bacillus subtilis = 2: 1.5: 66.46kg of mixed bacteria in a proportion of 1.5, drilling a small hole in a barrel cover containing liquid to be fermented, inserting a plastic hose with the inner diameter of 1.5cm, screwing the barrel cover, inserting the other end of the plastic hose into a water barrel containing clean water to facilitate the discharge of gas in the fermentation liquid, and fermenting for one week at the temperature of 28-30 ℃ to obtain the fermentation liquid for later use;

(11) taking 33kg of the filter residue mixed material obtained in the step (10), mixing the filter residue mixed material with 52kg of dried cow dung, bean pulp and rapeseed meal according to the weight ratio of 1: 1, adding dried streptococcus thermophilus powder with viable bacteria concentration of 200 hundred million per gram, dried bacillus subtilis powder with viable bacteria concentration of 200 hundred million per gram and dried candida tropicalis powder with viable bacteria concentration of 200 hundred million per gram according to a weight ratio of 1: 1: 8kg of composite bacteria starter mixed according to the proportion of 1, uniformly mixing, adding a proper amount of water to adjust the water content to 40-45 percent, and obtaining a solid culture base material;

Example 15

(1) selecting and selecting okra leaves, rabdosia rubescens leaves, tartary buckwheat leaves, agastache leaves and tea leaves of plant leaves, respectively drying, and mixing the okra leaves, the rabdosia rubescens leaves, the tartary buckwheat leaves, the agastache leaves and the tea leaves according to the weight ratio of 10: 10: 5: 1: 1 proportion, crushing into 80-mesh powder, weighing 100kg of powder, placing into a reaction kettle, adding 3000kg of purified water, soaking at normal temperature for 6 hours, fully stirring to obtain uniform slurry, adjusting the pH of the slurry to 4.8 by using a sulfuric acid solution, then adding 2945g of cellulase with the activity unit of 2000 mu per gram and 7750g of pectinase with the activity unit of 20000 mu per gram into the slurry, uniformly stirring, heating to 50 ℃, performing heat preservation and enzymolysis for 4.5 hours, and slightly stirring for 5 minutes every 60 minutes to obtain enzymolysis slurry;

(2) heating the slurry prepared in the step (1) to 100 ℃, boiling for 30min, naturally cooling, standing for 18h, filtering the standing slurry to obtain 3013.2kg of filtrate, taking the filtrate for later use, and filtering the residue for later use;

(3) mixing the soluble substances in the dry rice alcohol vinasse and the dry corn alcohol vinasse containing the soluble substances according to a weight ratio of 1: 1, drying the mixture by microwave, crushing the mixture into 80-mesh powder, weighing 100kg of the powder, placing the powder into a reaction kettle, adding 3000kg of purified water, soaking for 4 hours, fully and uniformly stirring to obtain uniform slurry, adjusting the pH to 5.0 by using a sulfuric acid solution, adding 2170g of cellulase with an activity unit of 2000 mu per gram, 930g of pectinase with an activity unit of 30000 mu per gram and 1860g of α -amylase with an activity unit of 6000 mu per gram into the slurry, uniformly stirring, heating to 50 ℃, carrying out heat preservation and enzymolysis for 30 hours, and slightly stirring for 10 minutes every 60 minutes during the enzymolysis to obtain enzymolysis slurry;

(4) heating the slurry prepared in the step (3) to 100 ℃, boiling for 30min, naturally cooling, standing for 24h, filtering the slurry after standing to obtain 3007kg of filtrate, taking the filtrate for later use, and filtering the residue for later use;

(6) adding the fermentation solution to be fermented in the step (5) into brewing yeast in a weight ratio of: lactobacillus bulgaricus: bacillus subtilis = 2: 1.5: 54.18g of mixed bacteria in a proportion of 1.5, drilling a small hole in a barrel cover containing liquid to be fermented, inserting a plastic hose with the inner diameter of 1.5cm, screwing the barrel cover, inserting the other end of the plastic hose into a water barrel containing clean water to facilitate the discharge of gas in the fermentation liquid, and fermenting for one week at the temperature of 28-30 ℃ to obtain the fermentation liquid for later use;

(9) continuously concentrating the concentrated solution with the specific weight of 2.25-5.25 in the step (7) into a dry extract, wherein the dry extract comprises the following components in parts by weight: starch: complex microorganism = 1: 4: 1, wherein the composite microbial bacteria are prepared from beer yeast dry powder containing 200 hundred million per gram, lactococcus lactis dry powder containing 200 hundred million per gram and bacillus licheniformis dry powder containing 200 hundred million per gram according to the weight ratio of 2: 1: 1, granulating by using a 12-mesh sieve, drying by cold air, and sieving by using a 16-mesh sieve to obtain the solid microbial feed additive for animals;

(11) taking 35kg of the filter residue mixed material obtained in the step (10), mixing with 50kg of dried rabbit manure, soybean germ meal, peanut meal and grape seed meal according to the weight ratio of 1: 1: 1, adding lactobacillus acidophilus dry powder with the viable bacteria concentration of 200 hundred million per gram, bacillus licheniformis dry powder with the viable bacteria concentration of 200 hundred million per gram and brewing yeast dry powder with the viable bacteria concentration of 200 hundred million per gram according to the weight ratio of 1: 1: 8kg of composite bacteria starter mixed according to the proportion of 1, uniformly mixing, adding a proper amount of water to adjust the water content to 40-45 percent, and obtaining a solid culture base material;

Example 16

(1) picking and selecting cassava leaves, tomato leaves and soybean leaves of plant leaves, respectively drying, and mixing the cassava leaves, the tomato leaves and the soybean leaves according to the weight ratio of 10: 5: crushing the powder into 80-mesh powder in a proportion of 5, weighing 100kg of the powder, placing the powder into a reaction kettle, adding 3000kg of purified water, soaking the powder for 6 hours at normal temperature, fully stirring to obtain uniform slurry, adjusting the pH of the slurry to 5.0 by using a sulfuric acid solution, then adding 2945g of cellulase with the activity unit of 2000 mu per gram and 7750g of pectinase with the activity unit of 20000 mu per gram into the slurry, uniformly stirring, heating to 50 ℃, performing heat preservation and enzymolysis for 4.5 hours, and slightly stirring for 5 minutes every 60 minutes to obtain enzymolysis slurry;

(3) after a mixture of dried white vinasse and dried yellow vinasse in a weight ratio of 1: 1 is subjected to microwave drying treatment, the mixture is crushed into 80-mesh powder, 100kg of the powder is weighed and placed into a reaction kettle, 3000kg of purified water is added, after the mixture is soaked for 4 hours, the mixture is fully and uniformly stirred to form uniform slurry, the pH value is adjusted to 5.0 by using a sulfuric acid solution, then 2325g of cellulase with an activity unit of 2000 mu per gram, 1705g of pectinase with an activity unit of 30000 mu per gram and 1860g of α -amylase with an activity unit of 6000 mu per gram are added into the slurry, the mixture is uniformly stirred, the mixture is heated to 55 ℃, heat preservation and enzymolysis are performed for 30 hours, and the mixture is lightly stirred for 10 minutes every 60 minutes during the enzymolysis, so;

(6) adding the liquid to be fermented in the step (5) into the mixture of red yeast: lactobacillus plantarum: bacillus coagulans = 2: 1.5: 48.28kg of mixed bacteria in a proportion of 1.5, drilling a small hole in a barrel cover containing liquid to be fermented, inserting a plastic hose with the inner diameter of 1.5cm, screwing the barrel cover, inserting the other end of the plastic hose into a water barrel containing clean water to facilitate the discharge of gas in the fermentation liquid, and fermenting for one week at the temperature of 28-30 ℃ to obtain the fermentation liquid for later use;

(11) taking 25kg of filter residue mixed material obtained in the step (10), and mixing the filter residue mixed material with dried rabbit manure, sheep manure and cow manure according to a weight ratio of 1: 1: 1, mixing 60kg of mixed manure with 7kg of cottonseed meal, and adding 200 hundred million lactobacillus acidophilus dry powder containing viable bacteria per gram, 200 hundred million bacillus subtilis dry powder containing viable bacteria per gram and 200 hundred million brewer's yeast dry powder containing viable bacteria per gram according to the weight ratio of 1: 1: 8kg of composite bacteria starter mixed according to the proportion of 1, uniformly mixing, adding a proper amount of water to adjust the water content to 40-45 percent, and obtaining a solid culture base material;

Claims

1. The comprehensive utilization method based on plant leaves and vinasse is characterized by comprising the following steps of:

(3) drying vinasse, crushing the vinasse into 80-mesh powder, adding water 30 times the weight of the vinasse into the powder, soaking the vinasse for 4 hours, fully stirring the mixture to form uniform slurry, adjusting the pH to 5.0 by using a sulfuric acid solution, then adding cellulase with the activity of 2000 mu/g in a weight range of 0.55-0.75 thousandth, pectinase with the activity unit of 30000 mu/g in a weight range of 0.25-0.55 thousandth and α -amylase with the activity unit of 6000 mu/g in a weight range of 0.4-0.6 thousandth into the slurry, uniformly stirring the mixture, heating the mixture to 50-60 ℃, carrying out heat preservation enzymolysis for 24-30 hours, and slightly stirring the mixture for 10 minutes every 60 minutes to prepare enzymolysis slurry;

(7) filtering the fermentation liquor obtained in the step (6) to respectively obtain filtrate and solid-phase precipitate, wherein the solid-phase precipitate is reserved; heating and concentrating the filtrate to a concentrated solution with the specific gravity of 2.25-5.25, then adding 5% of Streptococcus lactis (Streptococcus lactis) with the viable bacteria content concentration of not less than 200 hundred million per gram and 5% of Bacillus licheniformis (Bacillus licheniformis) with the viable bacteria content of not less than 200 hundred million per gram according to the weight ratio of the concentrated solution, uniformly mixing, subpackaging, and storing at the temperature of 1-2 ℃ to obtain the liquid feed additive product for animals.

2. The method for comprehensive utilization of plant leaves and distiller's grains according to claim 1, wherein the following steps are continued after the step (7) to obtain the active yeast enriched feed additive product for animals:

3. The method for comprehensive utilization of plant leaves and distiller's grains according to claim 1, wherein the following steps are continued after the step (7) to produce the solid microbial feed additive product for animals:

4. The comprehensive utilization method based on plant leaves and vinasse according to claim 1, characterized in that the following steps are continued after the step (2) to prepare a bio-organic fertilizer product;

5. The method for comprehensive utilization based on plant leaves and distiller's grains according to claim 1 or 2 or 3 or 4, wherein the plant leaves in step (1) are pumpkin leaves or watermelon leaves or white gourd leaves or cucumber leaves or luffa leaves or melon leaves or pumpkin leaves or cucurbit leaves or garlic leaves or ginger leaves or leek leaves or welsh onion leaves or celery leaves or cauliflower leaves or dried mosses leaves or lettuce leaves or radish leaves or carrot leaves or mustard leaves or capsicum leaves or solanum nigrum leaves or bud leaves or pea leaves or hyacinth bean leaves or broad bean leaves or soybean leaves or red bean leaves or sesame leaves or cassava leaves or burdock leaves or cloth dregs leaves or lotus rhizome leaves or water chestnut leaves or tomato leaves or sweet potato leaves or chicory leaves or jerusalem leaves or kom leaves or spinach leaves or cabbage leaves or taro leaves or yam leaves or tea leaves or water chestnut leaves or potato leaves or asparagus leaves or yellow grass leaves or black beans leaves or tomato leaves or asparagus leaves or yellow rice leaves or black beans leaves or tomato leaves Folium mume or ramie leaf or folium allii macrostemi or plantain leaf or folium Perillae or peony leaf or five-flavor cotyledon or dogwood leaf or pig hair leaf or ginseng leaf or radix et caulis Opuntiae Dillenii leaf or angelica sinensis leaf or cassia tora leaf or radix Ophiopogonis leaf or radix Salviae Miltiorrhizae leaf or bitter buckwheat leaf or barley leaf or wheat leaf or dandelion leaf or houttuynia cordata leaf or herba Schizonepetae leaf or safflower leaf or honeysuckle leaf or folium Ginkgo or aloe leaf or polygonum multiflorum leaf or konjac leaf or purslane stem leaf or radix Puerariae leaf or liquorice leaf or jujube leaf or russianolive leaf or fructus Ziziphi Spinosae leaf or fructus Hippophae leaf or fructus lycii leaf or peach leaf or apricot leaf or pine needle leaf or guava leaf or fructus momordicae leaf or peony leaf or folium Ilicis Purpureae or flos Chrysanthemi leaf or fructus fici leaf or chestnut leaf or semen Castaneae leaf or fructus alpiniae fruit leaf or apple leaf or pear leaf or folium Broussonetiae chinensis leaf or folium Brassicae campestris leaf or folium artemisiae chinensis leaf or folium ilicis leaf or folium ilicis Tagetes erecta leaf or maidenhair leaf or gardenia jasminoides leaf or paulownia leaf or poplar leaf or sunflower leaf or grape leaf or asparagus leaf or lablab album leaf or bighead atractylodes rhizome leaf or biota orientalis leaf or mint leaf or psoralea corylifolia leaf or atractylodes rhizome leaf or red peony root leaf or acanthopanax senticosus leaf or lophatherum gracile leaf or finger citron leaf or lesser galangal rhizome leaf or rhodiola root leaf or magnolia bark leaf or fenugreek leaf or sophora leaf or polygonatum rhizome leaf or asiatic centella leaf or gynostemma pentaphylla leaf or platycodon grandiflorum leaf or fagopyrum tataricum leaf or rose leaf or papaya leaf or myrcia oleracea leaf or privet folium or astragalus mongholicus leaf or rubia madder leaf or cardamon leaf or mulberry leaf or sour bean leaf or asparagus root leaf or acanthopanax senticosus leaf or motherwort leaf or alpinia oxyphylla leaf or stevia rebaudiana leaf or phyllostachys leaf or polygala leaf or cowberry leaf or sour orange leaf or tendril leaf or vine leaf or jerusalem artichoke leaf or bletilla striata leaf or sophora leaf or One or more of folium Lini, semen Sojae Atricolor, semen Phaseoli Radiati, cortex Cinnamomi, rhizoma Ligustici Chuanxiong, flos Celosiae Cristatae, folium Cnidii, folium Foeniculi, radix Morindae officinalis, folium Viticis negundo, folium Ulmi Pumilae, and folium Granati.

6. The method for comprehensive utilization based on plant leaves and distiller's grains according to claim 5, wherein said pumpkin leaves or watermelon leaves or white gourd leaves or cucumber leaves or towel gourd leaves or melon leaves or northern melon leaves or cucurbit leaves or gourd leaves or garlic leaves or ginger leaves or hotbed chives leaves or onion leaves or celery leaves or cauliflower leaves or dried moss leaves or lettuce leaves or radish leaves or carrot leaves or mustard leaves or hot pepper leaves or eggplant leaves or bud bean leaves or pea leaves or cowpea leaves or hyacinth bean leaves or broad bean leaves or soybean leaves or red bean leaves or sesame leaves or cassava leaves or burdock leaves or cloth dregs leaves or lotus root leaves or water chestnut leaves or sweet potato leaves or chicory leaves or jerusalem leaves or broom cypress leaves or cabbage leaves or spinach leaves or water chestnut leaves or oat leaves or tea leaves or yam leaves or ginger leaves or Chinese mugwort leaves or garlic leaves or asparagus leaves or black beans leaves or purple sweet potato leaves or asparagus leaves or black beans leaves or purple sweet potato leaves or black beans leaves or purple sweet potato leaves or purple flowers or black beans or purple flowers leaves Folium Perillae or folium moutan or folium Schisandrae chinensis or folium Corni or folium Salsolae Collinae or folium Ginseng or folium Codonopsis Lanceolatae or folium Angelicae sinensis or folium Cassiae or folium Ophiopogonis or folium Salviae Miltiorrhizae or folium Fagopyri Dibotryis or fructus Hordei Germinatus or folium Taraxaci or folium Houttuyniae or folium Schizonepetae or folium Carthami tinctorii or caulis Lonicerae or folium Ginkgo or folium Aloe or radix Polygoni Multiflori or folium Amorphophalli or caulis Portulacae or folium Puerariae Lobatae or folium Jujubae or folium Elaeagni Angustifoliae or folium Ziziphi Spinosae or folium Hippophae or folium Ilicis Punicae or folium Hippophae or folium Psidii Guajavae or folium Ilicis Rotundae or folium Et folium Artemisiae Argyi or folium Brassicae chinensis or folium Artemisiae Argyi or folium Melissae or folium Artemisiae Argyi or folium Melissae or folium Or poplar leaf or sunflower leaf or grape leaf or dragon's hair vine leaf or white hyacinth bean leaf or white atractylodes rhizome leaf or arborvitae twig or peppermint leaf or psoralea fruit leaf or atractylodes rhizome leaf or red peony root leaf or acanthopanax leaf or lophatherum gracile leaf or finger citron leaf or lesser galangal rhizome leaf or rhodiola rhizome leaf or magnolia bark leaf or fenugreek seed leaf or sophora flower leaf or polygonatum rhizome leaf or asiatic pennywort leaf or gynostemma pentaphyllum leaf or platycodon grandiflorum leaf or tartarian buckwheat leaf or rose flower leaf or papaya leaf or myrtle leaf or glossy privet leaf or astragalus mongholicus leaf or astragalus membranaceus leaf or madder leaf or cardamon leaf or mulberry leaf or sour bean leaf or asparagus leaf or acanthopanax bark leaf or common cephalanoplos leaf or common motherwort leaf or alpinia leaf or stevia rebaudiana leaf or yuba yunnanensis leaf or polygala yunnanensis leaf or bilberry leaf or orange leaf or vine leaf or sun flower leaf or gentian leaf or anemarrhena leaf or peanut leaf or black ginseng leaf or phellodendron bark leaf or hyacinth leaf or bletilla striata leaf or hyacinth bletilla hyacinosa leaf or black sophora leaf or black bean leaf or bletilla striata leaf or bletilla hyacinthinleaf or bletilla hyacinosa leaf or bletilla hyacin Or dry or fresh rhizoma Ligustici Chuanxiong leaf or folium Celosiae Cristatae or folium Cnidii or fructus Foeniculi leaf or radix Morindae officinalis leaf or folium Viticis negundo or folium Ulmi Pumilae or folium Granati.

7. The method for comprehensive utilization based on plant leaves and distiller's grains according to claim 6, wherein said pumpkin leaves or watermelon leaves or white gourd leaves or cucumber leaves or towel gourd leaves or melon leaves or northern melon leaves or cucurbit leaves or gourd leaves or garlic leaves or ginger leaves or hotbed chives leaves or onion leaves or celery leaves or cauliflower leaves or dried moss leaves or lettuce leaves or radish leaves or carrot leaves or mustard leaves or hot pepper leaves or eggplant leaves or bud bean leaves or pea leaves or cowpea leaves or hyacinth bean leaves or broad bean leaves or soybean leaves or red bean leaves or sesame leaves or cassava leaves or burdock leaves or cloth dregs leaves or lotus root leaves or water chestnut leaves or sweet potato leaves or chicory leaves or jerusalem leaves or broom cypress leaves or cabbage leaves or spinach leaves or water chestnut leaves or oat leaves or tea leaves or yam leaves or ginger leaves or Chinese mugwort leaves or garlic leaves or asparagus leaves or black beans leaves or purple sweet potato leaves or asparagus leaves or black beans or purple potatoes or purple sweet potato leaves or purple sweet potato leaves or black beans or black leaves or black beans leaves or purple sweet potato leaves or black beans or purple leaves or purple flowers or black beans or purple flowers leaves or black beans or purple sweet potato Folium Perillae or folium moutan or folium Schisandrae chinensis or folium Corni or folium Salsolae Collinae or folium Ginseng or folium Codonopsis Lanceolatae or folium Angelicae sinensis or folium Cassiae or folium Ophiopogonis or folium Salviae Miltiorrhizae or folium Fagopyri Dibotryis or fructus Hordei Germinatus or folium Taraxaci or folium Houttuyniae or folium Schizonepetae or folium Carthami tinctorii or caulis Lonicerae or folium Ginkgo or folium Aloe or radix Polygoni Multiflori or folium Amorphophalli or caulis Portulacae or folium Puerariae Lobatae or folium Jujubae or folium Elaeagni Angustifoliae or folium Ziziphi Spinosae or folium Hippophae or folium Ilicis Punicae or folium Hippophae or folium Psidii Guajavae or folium Ilicis Rotundae or folium Et folium Artemisiae Argyi or folium Brassicae chinensis or folium Artemisiae Argyi or folium Melissae or folium Artemisiae Argyi or folium Melissae or folium Or poplar leaf or sunflower leaf or grape leaf or dragon's hair vine leaf or white hyacinth bean leaf or white atractylodes rhizome leaf or arborvitae twig or peppermint leaf or psoralea fruit leaf or atractylodes rhizome leaf or red peony root leaf or acanthopanax leaf or lophatherum gracile leaf or finger citron leaf or lesser galangal rhizome leaf or rhodiola rhizome leaf or magnolia bark leaf or fenugreek seed leaf or sophora flower leaf or polygonatum rhizome leaf or asiatic pennywort leaf or gynostemma pentaphyllum leaf or platycodon grandiflorum leaf or tartarian buckwheat leaf or rose flower leaf or papaya leaf or myrtle leaf or glossy privet leaf or astragalus mongholicus leaf or astragalus membranaceus leaf or madder leaf or cardamon leaf or mulberry leaf or sour bean leaf or asparagus leaf or acanthopanax bark leaf or common cephalanoplos leaf or common motherwort leaf or alpinia leaf or stevia rebaudiana leaf or yuba yunnanensis leaf or polygala yunnanensis leaf or bilberry leaf or orange leaf or vine leaf or sun flower leaf or gentian leaf or anemarrhena leaf or peanut leaf or black ginseng leaf or phellodendron bark leaf or hyacinth leaf or bletilla striata leaf or hyacinth bletilla hyacinosa leaf or black sophora leaf or black bean leaf or bletilla striata leaf or bletilla hyacinthinleaf or bletilla hyacinosa leaf or bletilla hyacin Or dry leaf of rhizoma Ligustici Chuanxiong, leaf of cockscomb, leaf of cnidium monnieri (L.) DC, leaf of Foeniculum vulgare, leaf of Morinda officinalis, leaf of negundo chastetree, leaf of Ulmus davidiana var, or leaf of punica granatum.

8. The method according to claim 1 or 2 or 3 or 4, wherein the distiller's grains in step (3) are one or more of dried distiller's grains, dried yellow distiller's grains, dried brewer's grains, dried distillers ' grains [ DDG ], dried distillers ' grains solubles [ DDS ], dried distillers ' grains with whole spirit [ DDGS ], wet distillers ' grains [ DWG ] and wet distillers ' grains solubles [ DWS ].

9. The method for comprehensive utilization of plant leaves and distiller's grains according to claim 8, wherein the dry distillers ' grains [ DDG ] are any one of commercial dry distillers ' grains of barley, rice, corn, sorghum, wheat, rye, grains and potatoes; the dry alcohol tank soluble substance [ DDS ] is any one of a barley dry alcohol tank soluble substance, a rice dry alcohol tank soluble substance, a corn dry alcohol tank soluble substance, a sorghum dry alcohol tank soluble substance, a wheat dry alcohol tank soluble substance, a rye dry alcohol tank soluble substance, a grain dry alcohol tank soluble substance and a potato dry alcohol tank soluble substance which are sold in the market; the dry total distillers ' grains [ DDGS ] are any one of barley dry distillers ' grains containing soluble substances, rice dry distillers ' grains containing soluble substances, corn dry distillers ' grains containing soluble substances, sorghum dry distillers ' grains containing soluble substances, wheat dry distillers ' grains containing soluble substances, rye dry distillers ' grains containing soluble substances, cereal dry distillers ' grains containing soluble substances and potato dry distillers ' grains containing soluble substances which are sold in the market; the wet distillers ' grains [ DWG ] are any one of barley wet distillers ' grains, rice wet distillers ' grains, corn wet distillers ' grains, sorghum wet distillers ' grains, wheat wet distillers ' grains, rye wet distillers ' grains, cereal wet distillers ' grains and potato wet distillers ' grains sold in the market; the wet distillers ' grains soluble substance [ DWS ] is any one of barley wet distillers ' grains soluble substance, rice wet distillers ' grains soluble substance, corn wet distillers ' grains soluble substance, sorghum wet distillers ' grains soluble substance, wheat wet distillers ' grains soluble substance, rye wet distillers ' grains soluble substance, grain wet distillers ' grains soluble substance and potato wet distillers ' grains soluble substance which are commercially available.

10. The method of claim 9, wherein the grain stillage product is a dry grain stillage, a dry grain stillage solubles, a dry grain stillage with solubles, a wet grain stillage and a wet grain stillage solubles obtained from two or more types of grain seeds.

11. The comprehensive utilization method based on plant leaves and vinasse according to claim 1, wherein the drying in the step (3) comprises hot air drying, far infrared drying and microwave drying; preferably microwave drying is used.

12. The method for comprehensively utilizing plant leaves and distiller's grains according to claim 1, wherein the mixed bacteria in step (6) are yeast (Saccharomyces), lactobacillus (Lactobacillus) and Bacillus (Bacillus) in weight ratio: lactic acid bacteria: bacillus = 2: 1.5: 1.5 in proportion.

13. The method of claim 12, wherein the yeast (Saccharomyces) is any one of commercially available Candida utilis (Candida utilis), brewer's yeast (Saccharomyces cerevisiae), Rhodotorula rubra (Rhodotorula rubra), brewer's yeast (Saccharomyces cerevisiae) or fodder yeast (feed yeast); the Lactobacillus (Lactobacillus) is one or any combination of more than two of Lactobacillus fermentum (Lactobacillus fermentum), Streptococcus lactis (Streptococcus lactis), Lactobacillus lactis (Lactobacillus lactis), Lactobacillus plantarum (Lactobacillus plantarum) and Lactobacillus bulgaricus (Lactobacillus bulgaricus); the Bacillus (Bacillus) is one or more of Bacillus licheniformis (Bacillus licheniformis), Bacillus lentus (Bacillus lentus), Bacillus pumilus (Bacillus pumilus), Bacillus subtilis (Bacillus subtilis) and Bacillus coagulans (Bacillus coagulans) in any combination.

14. The method for comprehensive utilization of plant leaves and distiller's grains according to claim 2, wherein the yeast powder in the step (8) is any one of brewer's yeast, candida and feed yeast.

15. The method for comprehensive utilization of plant leaves and distiller's grains according to claim 3, wherein the composite microorganism strain of step (9) is prepared from yeast, lactic acid bacteria and bacillus by weight: lactic acid bacteria: bacillus = 2: 1: 1 proportion of the raw materials.

16. The method for comprehensive utilization of plant leaves and distiller's grains according to claim 15, wherein the yeast is any one of commercially available edible or feeding Candida utilis (Candida utilis), brewer's yeast (cerevisiaereferrum) or feed yeast (feed yeast); it is preferable to use a feed yeast (feed yeast); the Lactobacillus is one or any combination of more than two of Streptococcus thermophilus (Streptococcus thermophilus), Lactobacillus thermophilus (Lactobacillus thermophilus), Lactobacillus plantarum (Lactobacillus plantarum), lactococcus lactis (Lactobacillus lactis) and Lactobacillus bulgaricus (Lactobacillus bulgaricus); the Bacillus is one or the combination of more than two of Bacillus subtilis, Bacillus licheniformis, Bacillus coagulans, Bacillus stearothermophilus and Bacillus firmus.

17. The comprehensive utilization method based on plant leaves and vinasse as claimed in claim 4, wherein the animal manure in step (1) is any one or more of mixed manure of sheep manure, pig manure, cow manure, horse manure, donkey manure, camel manure and rabbit manure; the animal manure is subjected to drying treatment at 150 ℃.

18. The method for comprehensive utilization of plant leaves and distiller's grains according to claim 17, wherein the animal manure is mixed manure of sheep manure, cow manure, rabbit manure, or any combination thereof.

19. The method according to claim 4, wherein the oil seed meal in step (11) is one or a mixture of almond meal, rapeseed meal, canola meal, soybean germ meal, soybean meal, tomato seed meal, olive meal, walnut kernel meal, pecan kernel meal, safflower seed meal, pepper seed meal, peanut meal, sunflower seed meal, cottonseed meal, wood cottonseed meal, grape seed meal, sea buckthorn seed meal, wild jujube meal, shinyleaf yellowhorn seed meal, linseed meal, coconut meal, palm seed meal, evening primrose seed meal, sesame meal, perilla seed meal, capsicum seed meal, corn germ meal, wheat germ meal, and rice bran meal.

20. The method for comprehensive utilization of plant leaves and distiller's grains according to claim 4, wherein the composite bacteria starter in step (11) is prepared from lactic acid bacteria, bacillus and yeast by weight: bacillus: yeast = 1: 1: 1 proportion of the components.

21. The method for comprehensive utilization of plant leaves and distiller's grains according to claim 20, wherein the lactic acid bacteria are one or more of Streptococcus thermophilus (Streptococcus thermophilus), Streptococcus lactis (Streptococcus acidophilus), Lactobacillus acidophilus (Lactobacillus delbri), Lactobacillus delbri, and Lactobacillus reuteri (Lactobacillus rentdril); the Bacillus is one or the arbitrary combination of more than two of Bacillus licheniformis (Bacillus licheniformis), Bacillus circulans (Bacillus circulans Jordan), Bacillus subtilis (Bacillus subtilis), Bacillus firmus (Bacillus firmus) and Bacillus lentus (Bacillus lentus); the yeast is any one of brewers yeast (Saccharomyces cerevisiae), Candida utilis (Candida utilis) and Candida tropicalis (Candida tropicalis).

22. The comprehensive utilization method based on plant leaves and vinasse according to claim 4, wherein the compound humate in the step (13) is sodium humate, potassium humate and ammonium humate, and the compound humate is sodium humate: potassium humate: ammonium humate = 1: 1.5: 2, and mixing the components in a ratio of 2.

23. The method for comprehensively utilizing plant leaves and distiller's grains according to claim 1, 2, 3 or 4, wherein the yeast, lactic acid bacteria and bacillus are commercially available dry yeast powder products with viable bacteria concentration of not less than 200 million per gram, dry lactic acid bacteria powder products with viable bacteria concentration of not less than 200 million per gram and dry bacillus powder products with viable bacteria concentration of not less than 200 million per gram.