CN108743739A - A kind of preparation method of the active peptide of enhancing milk cow immunity - Google Patents
A kind of preparation method of the active peptide of enhancing milk cow immunity Download PDFInfo
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- CN108743739A CN108743739A CN201810680422.6A CN201810680422A CN108743739A CN 108743739 A CN108743739 A CN 108743739A CN 201810680422 A CN201810680422 A CN 201810680422A CN 108743739 A CN108743739 A CN 108743739A
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- active peptide
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- milk cow
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- HUTYZQWCTWWXND-NCTFTGAASA-N taraxasterol Natural products C[C@H]1[C@H]2C3=CC[C@@H]4[C@@]5(C)CC[C@H](O)C(C)(C)[C@@H]5CC[C@@]4(C)[C@]3(C)C[C@H](O)[C@@]2(C)CCC1=C HUTYZQWCTWWXND-NCTFTGAASA-N 0.000 description 1
- GGGUGZHBAOMSFJ-GADYQYKKSA-N taraxerol Chemical compound CC([C@@H]1CC2)(C)[C@@H](O)CC[C@]1(C)[C@@H]1[C@]2(C)C2=CC[C@@]3(C)CCC(C)(C)C[C@H]3[C@]2(C)CC1 GGGUGZHBAOMSFJ-GADYQYKKSA-N 0.000 description 1
- HYTFUKZLSYXRDX-UHFFFAOYSA-N taraxerol Natural products CC1CCCC2C3(C)CCC4(C)C5CC(C)(C)CCC5(C)C=CC4(C)C3C(O)CC12C HYTFUKZLSYXRDX-UHFFFAOYSA-N 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
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Abstract
The present invention relates to traditional Chinese medicine and biotechnology technical fields, specifically disclose a kind of preparation method of the active peptide of enhancing milk cow immunity.The preparation method, includes the following steps：By brassicae chinensis,semen, dandelion, Radix Astragali, Snakegourd Fruit, Chinese violet, river glutinous rehmannia, Wang Buliu, Radix Angelicae Sinensis, honeysuckle, cordate houttuynia, carries out immersion treatment with warm water, decoction processing, alcohol precipitation processing, is dried, obtain Chinese medical extract；The Chinese medical extract and chitosan, vitamin E powder and probiotics are subjected to mixing treatment, obtain active peptide.Preparation method provided by the invention, it is simple for process, it is easy to operate, it is environmentally protective, securely and reliably, and obtained active peptide, immunity of organisms is improved, mastitis for milk cows is prevented and treat, reduces incidence, reduces the body cell in milk, improves the output of milk.
Description
Technical field
The present invention relates to traditional Chinese medicine and biotechnology technical field more particularly to a kind of active peptides of enhancing milk cow immunity
Preparation method.
Background technology
Body cell (SCC) in milk is mainly to be made of leucocyte and the galactophore epithelial cell to fall off.Leucocyte has
Anti-disease infects and assists the defense function of damaged tissue repair.The rising of somatic number in milk, it is meant that the resistance of milk cow
The proportion of the decline of power, the increase of milk loss, recessive mastitis and clinical mammitis will rise.
Currently, body cell has been included in milk Prognostic factors in milk, each pasture is increasingly heavier to the control of body cell
Depending on.In addition to control breast bacterial infection, pay attention to milking other than standard operation, the feed addition of artificial addition biologically active polypeptide
Agent enhances the resistance of milk cow, reduces a kind of development trend that milk somatic cell is following.
Invention content
Decline for the resistance of existing milk cow, recessive mastitis and clinical mammitis occurs, causes body in milk thin
The problems such as rising of born of the same parents' number and milk loss amount increase, the present invention provide a kind of system of the active peptide of enhancing milk cow immunity
Preparation Method.
To achieve the above object of the invention, the embodiment of the present invention uses the following technical solution：
A kind of preparation method of the active peptide of enhancing milk cow immunity, includes the following steps：
(1) by 300-500 parts of brassicae chinensis,semens, 500-1000 portions of dandelions, 100-200 parts of Radix Astragalis, 100-150 parts of Snakegourd Fruits,
100-150 portions of Chinese violets first carry out immersion treatment with warm water, then carry out decoction processing, obtain the first extracting solution；
(2) 100-150 parts of river glutinous rehmannias, 100-150 parts of kings are not stayed, 80-150 parts of Radix Angelicae Sinensis, 80-200 parts of honeysuckles, 100-
300 portions of cordate houttuynias first carry out immersion treatment with warm water, then carry out decoction processing, obtain the second extracting solution；
(3) after first extracting solution being carried out concentration, concentrate is obtained, alcohol precipitation processing is carried out, obtains first and carry
Take object；After second extracting solution pH is adjusted to 5.5-6.5, alcohol precipitation processing is carried out, the second extract is obtained；Merge the first extract,
Second extract obtains Chinese medical extract through being dried；
(4) Chinese medical extract and 10-20 parts of chitosans, 8-15 parts of vitamin E powders and probiotics are subjected to batch mixing
Processing, it is 1.0 × 10 to make the viable bacteria amount in system6-1.0×109Cfu/g obtains active peptide.
Compared with the existing technology, the preparation method of the active peptide of enhancing milk cow immunity provided by the invention, use are a variety of
Chinese medicine reasonable compatibility, toxic side effect is small, noresidue, is not likely to produce drug resistance, and deliquescent not according to main component in Chinese medicine
Together, it extracts in different ways in batches, fully extracts the active peptides and other active ingredients in Chinese medicine, a variety of work
Property peptide, integrate " increasing breast, health care, safely ", complementation collaboration, improve immunity of organisms, prevent and treat mastitis for milk cows, drop
Low incidence reduces the body cell in milk, improves the output of milk, and this preparation method is simple for process, easy to operate, green ring
It protects, securely and reliably.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The embodiment of the present invention provides a kind of preparation method of the active peptide of enhancing milk cow immunity.The preparation method, including
Following steps：
(1) by 300-500 parts of brassicae chinensis,semens, 500-1000 portions of dandelions, 100-200 parts of Radix Astragalis, 100-150 parts of Snakegourd Fruits,
100-150 portions of Chinese violets first carry out immersion treatment with warm water, then carry out decoction processing, obtain the first extracting solution；
(2) 100-150 parts of river glutinous rehmannias, 100-150 parts of kings are not stayed, 80-150 parts of Radix Angelicae Sinensis, 80-200 parts of honeysuckles, 100-
300 portions of cordate houttuynias first carry out immersion treatment with warm water, then carry out decoction processing, obtain the second extracting solution；
(3) after first extracting solution being carried out concentration, concentrate is obtained, alcohol precipitation processing is carried out, obtains first and carry
Take object；After second extracting solution pH is adjusted to 5.5-6.5, alcohol precipitation processing is carried out, the second extract is obtained；Merge the first extract,
Second extract obtains Chinese medical extract through being dried；
(4) Chinese medical extract and 10-20 parts of chitosans, 8-15 parts of vitamin E powders and probiotics are subjected to batch mixing
Processing, it is 1.0 × 10 to make the viable bacteria amount in system6-1.0×109Cfu/g obtains active peptide.
Specifically, chitosan has the immunoregulation effect to body, the function of strengthen immunity, and chitosan and its spreads out
Biology has preferable antibacterial activity, can inhibit the growth and breeding of some fungies, bacterium and virus, at the same time it can also reduce milk cow
Neutral fat in breast and low-density lipoprotein promote the breeding of milk cow enteral beneficial bacterium, and then enhance it to nutriment
It absorbs；And vitamin E powder can enhance immunity of organisms, with Chinese medicine collective effect, achieve the purpose that improve milk cow immunity of organisms.
Preferably, the probiotics is probiotics powder agent, wherein including：Bacillus subtilis 20-30 hundred million/gram, acidophilus breast
Bacillus 1-3 hundred million/gram, bacillus licheniformis 25-32 hundred million/gram, candida utili bacterium 3-5 hundred million/gram, it is enough to ensure that probiotics has
Viable bacteria amount is completed to ferment to the depth of other components.
Preferably, in step (1), the immersion treatment is 2 times, uses the water of 9-11 times of Chinese medicine weight for the first time, for the second time
With 7-9 times of water of Chinese medicine weight, immersion treatment temperature is 30-37 DEG C twice, and the time is 25-35min；In step (2), institute
It is 3 times to state immersion treatment, and the water of 9-11 times of Chinese medicine weight, second of the water for using 7-9 times of Chinese medicine weight is used to use for the third time for the first time
The water of 5-7 times of Chinese medicine weight, immersion treatment temperature is 30-37 DEG C three times, and the time is to be decocted convenient for subsequent for 1.0-1.5h
Processing is boiled, the extraction of extract is more advantageous to.
Preferably, in step (1), decoction processing is 2 times, and time first time is 1.2-1.8h, and second of time is
0.8-1.2h；In step (2), the decoction processing is 3 times, and time first time is 1.5-2.5h, and second of time is 0.8-
1.2h, third time time are 0.8-1.2h, ensure that extraction is abundant.
Preferably, in step (1), (2), the decoction processing is heated using steam, and vapour pressure is 0.15-0.25MP,
Heat up more uniform and stable, the active ingredient in survivable Chinese medicine.
Preferably, the first extracting solution relative density is 1.2-1.25 after the concentration, for the ease of subsequent alcohol precipitation
Processing.
Preferably, pH oxalic acid (concentration 10%) or sodium bicarbonate (concentration 7%) are adjusted, the dissolubility of extract is improved,
Convenient for alcohol precipitation.
Preferably, in the alcohol precipitation processing, the ethyl alcohol of its 1-4 times of volume is added into concentrate or extracting solution, ensures to fill
Divide the impurity component in removing extracting solution.
Preferably, in the alcohol precipitation processing, by containing alcohol liquid in 5-10 DEG C, 60-75h is stood, ensures Chinese medical extract
Sufficient crystallising is precipitated.
Preferably, in step (1), (2), the warm water used in the immersion treatment is heated up to obtain by injection stage with water, injection
Grade water is handled to obtain purified water by drinking water by a two-pass reverse osmosis, and purified water is handled using distillation, obtains injection stage
Use water.Securely and reliably.
The main chemical compositions and pharmacology of Chinese medicine used in the present invention are as follows：
Brassicae chinensis,semen, chemical composition mainly have：Fat 40~50%, protein 23%, and there have a kind of glucoside that can generate to be different thio
Cyanic acid crotons alcohol ester, thiocyanate ion (SCN-) precursor, by brassicae chinensis,semen itself and the glucose in digestive juice in alimentary canal
Glycosides phenol hydrolyzes, and forms SCN-, entered in milk by blood, in milk peroxidase (LP) and the ridges Huang Ca oxidizing ferment and
The hydrogen peroxide that certain bacteriums itself generate constitutes LP/SCN-/H2O2Disinfection system, i.e. H2O2SCN is aoxidized under the catalysis of LP-,
Generate time rhodanate (OSCN-), OSCN-Continue oxidizing bacteria cell surface and carry-SH enzymes, such as bluff pool acidohydrogenase, lactic acid is de-
Hydrogen enzyme etc. is allowed to inactivate, and then inhibits the biosynthesis of bacteria cell wall, and plays certain bactericidal effect.In addition, brassicae chinensis,semen
With promoting blood circulation scattered silt effect, and under opsonification, there is the effect for promoting leucocyte to reinforce phagocytosis.
Dandelion contains taraxasterol, taraxerol, stigmasterol, sitosterol, choline, organic acid, synanthrin, rubber
Deng having significant inhibiting effect to gold-coloured staphylococci, staphylococcus epidermis, hemolytic streptococcus, micrococcus catarrhalis, have wide
Compose bacteriostasis, antibacterial action mechanism be on the one hand by inhibit Cell wall synthesis, be on the other hand by inhibit protein and
DNA's synthesizes to realize.
Cordate houttuynia, acrid flavour are slightly cold, distributed in lung channel, have the function of the clearing heat and detoxicating, carbuncle that disappears apocenosis, inducing diuresis for treating strangurtia, are mainly used for
Lung carbuncle pyemesis, phlegm heat panting cough etc..Cordate houttuynia complex chemical composition mainly contains volatile oil, alkaloid, flavones, polysaccharide, organic acid
Deng with preferable anti-inflammatory activity.
Honeysuckle is rich in volatile oil, contains flavonoids, triterpenes and organic acid etc. in addition, honeysuckle have broad-spectrum antiseptic,
The effects that antiviral, enhancing is immune and antipyretic and anti-inflammatory.
Radix Angelicae Sinensis includes mainly the ingredients such as volatile oil, organic acid, polysaccharide and flavones, Radix Angelicae Sinensis polysaccharide (Angelica
Polysaccharide, APS) it is the main active that Radix Angelicae Sinensis plays immunization, specific immunity and non-specificity are exempted from
Epidemic disease has stronger facilitation, and can enhance mononuclear phagocyte system cleans up function, can enhance gulping down for peritoneal macrophage
Function is bitten, and then enhances immune function；In addition, angelica extract also has analgesia, anti-inflammatory effect.
Radix Astragali, including astragalus polyose, astragaloside, Radix Astragali flavone constituents etc. have strengthen immunity and antiviral
Effect, wherein astragalus polyose can stimulate immune system in many aspects, on the one hand, can increase in lymphatic system and marrow
The quantity of stem cell, it is active immunocyte to promote these stem cell transformations；On the other hand, also thin to improving normal macrophage
The phagocytic function of born of the same parents, the activity of natural killer cells (NK), the differentiation and maturation for promoting the immunocytes such as antibody formation and T cell
Deng there is remarkable effect.Astragaloside IV all has an impact T, B lymphocyte proliferation, Peritoneal Macrophages Function etc..Pass through inhibition
NF-KThe expression of B activity and adhesion molecule plays anti-inflammatory effect.
River glutinous rehmannia, containing volatile oil, alkaloid, phenolic substance, organic acid, phthalide lactone and other etc. ingredients, have it is antibacterial,
Antiviral, inhibition inflammatory reaction, the effect of adjusting immune function, reinforce immunity of organism, improve resistance.
Snakegourd Fruit includes mainly grease type, sterols, flavonoids, triterpenes and amino acid, protein etc., Snakegourd Fruit Aqueous extracts
There is preferable bacteriostasis, the conversion of macrophage activity and lymphocyte can be significantly improved, and then reaches the immune work(of enhancing
Effect.
King does not stay, its major function of Chinese herbal medicine is to increase breast, is played an important roll to mammogenesis and lactation, wherein effectively
Ingredient is dibutyl phthalate.
Chinese violet contains more than 10 kinds of alkaloid.From wherein obtaining 4 kinds of corydlis bungeana A prime, B prime, C prime and D prime different lifes
Alkaloids.Another lactone containing Coumarins, steroid saponin, phenolic substance, resinene and volatile oil etc., have antibacterial, anti-inflammatory effect.
This method uses plurality of Chinese reasonable compatibility, and toxic side effect is small, noresidue, is not likely to produce drug resistance, and in
The deliquescent difference of main component, extracts in different ways in batches in medicine, and the activity fully extracted in Chinese medicine is more
Peptide and other active ingredients, various active peptide integrate " increasing breast, health care, safely ", complementation collaboration, raising immunity of organisms,
Mastitis for milk cows is prevented and treated, incidence is reduced, reduces the body cell in milk, improves the output of milk, and this preparation method work
Skill is simple, easy to operate, environmentally protective, securely and reliably.
In order to better illustrate the preparation method of the active peptide provided in an embodiment of the present invention for enhancing milk cow immunity, below
It is illustrated by the way that embodiment is further.
Embodiment 1
A kind of preparation method of the active peptide of enhancing milk cow immunity.The preparation method, includes the following steps：
(1) 500 grams of brassicae chinensis,semens, 1000 grams of dandelions, 200 grams of Radix Astragalis, 150 grams of Snakegourd Fruits, 150 grams of Chinese violets carry for 2 times
It takes, uses the injection stage water of 10 times of Chinese medicine weight for the first time, after 37 DEG C carry out immersion treatment 25min, heated and decocted using steam
Extraction 1.2h is boiled, second of the injection stage water for using 8 times of Chinese medicine weight decocts extraction after 37 DEG C carry out immersion treatment 25min
0.8h merges extracting solution, obtains the first extracting solution；
(2) 150 grams of river glutinous rehmannias, 150 grams of kings are not stayed, 150 grams of Radix Angelicae Sinensis, 200 grams of honeysuckles, 300 grams of cordate houttuynias carry for 3 times
It takes, uses the injection stage water of 10 times of Chinese medicine weight for the first time, after 37 DEG C carry out immersion treatment 1.0h, heated and decocted using steam
1.5h is extracted, extracting solution is collected, uses the injection stage water of 8 times of Chinese medicine weight for the second time, after 37 DEG C carry out immersion treatment 1.0h,
Extraction 0.8h is decocted, extracting solution is collected, uses the injection stage water of 6 times of Chinese medicine weight for the third time, immersion treatment is carried out in 37 DEG C
After 1.0h, 0.8h is extracted, merges extracting solution, obtains the second extracting solution；
(3) after being 1.2 by first extracting solution progress concentration to relative density, concentrate is obtained, to concentrate
The middle ethyl alcohol that 1 times of volume of concentrate is added, carries out alcohol precipitation processing, by containing alcohol liquid in 5-10 DEG C, stands 60h, filtering obtains the
One extract；Second extracting solution oxalic acid (concentration 10%) or sodium bicarbonate (concentration 7%), it is 6.5 to adjust pH, in changing wherein
The ethyl alcohol of 1.5 times of volumes of extracting solution is added into extracting solution for the dissolubility of medicine ingredient and impurity, alcohol precipitation processing is carried out, by containing alcohol
Liquid stands 60-75h in 5-10 DEG C, and filtering obtains the second extract；Merge the first extract, the second extract, in 50 DEG C
Dry 45min, obtains Chinese medical extract；
(4) by the Chinese medical extract and 20 grams of chitosans, 15 grams of vitamin E powders and by following probiotic group at benefit
Raw bacteria powder：Bacillus subtilis 2,000,000,000/gram, lactobacillus acidophilus 100,000,000/gram, bacillus licheniformis 2,500,000,000/gram, Candida utilis ferment
Female bacterium 300,000,000/gram, mixing treatment is carried out, it is 1.0 × 10 to make the viable bacteria amount in system6Cfu/g obtains active peptide.
Embodiment 2
A kind of preparation method of the active peptide of enhancing milk cow immunity.The preparation method, includes the following steps：
(1) 400 grams of brassicae chinensis,semens, 800 grams of dandelions, 150 grams of Radix Astragalis, 120 grams of Snakegourd Fruits, 120 grams of Chinese violets carry for 2 times
It takes, uses the injection stage water of 9 times of Chinese medicine weight for the first time, after 37 DEG C carry out immersion treatment 35min, heated and decocted using steam
1.5h is extracted, second of the injection stage water for using 7 times of Chinese medicine weight decocts extraction after 37 DEG C carry out immersion treatment 35min
1.0h merges extracting solution, obtains the first extracting solution；
(2) 120 grams of river glutinous rehmannias, 120 grams of kings are not stayed, 100 grams of Radix Angelicae Sinensis, 100 grams of honeysuckles, 200 grams of cordate houttuynias carry for 3 times
It takes, uses the injection stage water of 9 times of Chinese medicine weight for the first time, after 30 DEG C carry out immersion treatment 1.0h, heated and decocted using steam
2h is extracted, extracting solution is collected, second of the injection stage water for using 7 times of Chinese medicine weight is decocted after 30 DEG C carry out immersion treatment 1.0h
Extraction 1h is boiled, extracting solution is collected, uses the injection stage water of 5 times of Chinese medicine weight for the third time, after 30 DEG C carry out immersion treatment 1.0h,
1h is extracted, merges extracting solution, obtains the second extracting solution；
(3) after being 1.25 by first extracting solution progress concentration to relative density, concentrate is obtained, to concentrate
The middle ethyl alcohol that 1 times of volume of concentrate is added, carries out alcohol precipitation processing, by containing alcohol liquid in 5-10 DEG C, stands 75h, filtering obtains the
One extract；Second extracting solution oxalic acid (concentration 10%) or sodium bicarbonate (concentration 7%), it is 6.5 to adjust pH, in changing wherein
The ethyl alcohol of 1.5 times of volumes of extracting solution is added into extracting solution for the dissolubility of medicine ingredient and impurity, alcohol precipitation processing is carried out, by containing alcohol
Liquid stands 60h in 5-10 DEG C, and filtering obtains the second extract；Merge the first extract, the second extract, in 50 DEG C of dryings
45min obtains Chinese medical extract；
(4) by the Chinese medical extract and 15 grams of chitosans, 10 grams of vitamin E powders and by following probiotic group at benefit
Raw bacteria powder：Bacillus subtilis 2,500,000,000/gram, lactobacillus acidophilus 200,000,000/gram, bacillus licheniformis 3,000,000,000/gram, Candida utilis ferment
Female bacterium 400,000,000/gram, mixing treatment is carried out, it is 1.0 × 10 to make the viable bacteria amount in system7Cfu/g obtains active peptide.
Embodiment 3
A kind of preparation method of the active peptide of enhancing milk cow immunity.The preparation method, includes the following steps：
(1) 300 grams of brassicae chinensis,semens, 500 grams of dandelions, 100 grams of Radix Astragalis, 100 grams of Snakegourd Fruits, 100 grams of Chinese violets carry for 2 times
It takes, uses the injection stage water of 11 times of Chinese medicine weight for the first time, after 35 DEG C carry out immersion treatment 25min, heated and decocted using steam
Extraction 1.8h is boiled, second of the injection stage water for using 9 times of Chinese medicine weight decocts extraction after 35 DEG C carry out immersion treatment 25min
1.2h merges extracting solution, obtains the first extracting solution；
(2) 100 grams of river glutinous rehmannias, 100 grams of kings are not stayed, 80 grams of Radix Angelicae Sinensis, 80 grams of honeysuckles, 100 grams of cordate houttuynias carry for 3 times
It takes, uses the injection stage water of 11 times of Chinese medicine weight for the first time, after 37 DEG C carry out immersion treatment 1.0h, heated and decocted using steam
2.5h is extracted, extracting solution is collected, uses the injection stage water of 9 times of Chinese medicine weight for the second time, after 37 DEG C carry out immersion treatment 1.0h,
Extraction 1.2h is decocted, extracting solution is collected, uses the injection stage water of 7 times of Chinese medicine weight for the third time, immersion treatment is carried out in 37 DEG C
After 1.0h, 1.2h is extracted, merges extracting solution, obtains the second extracting solution；
(3) after being 1.2 by first extracting solution progress concentration to relative density, concentrate is obtained, to concentrate
The middle ethyl alcohol that 1 times of volume of concentrate is added, carries out alcohol precipitation processing, by containing alcohol liquid in 5-10 DEG C, stands 70h, filtering obtains the
One extract；Second extracting solution oxalic acid (concentration 10%) or sodium bicarbonate (concentration 7%), it is 6.0 to adjust pH, in changing wherein
The ethyl alcohol of 1.5 times of volumes of extracting solution is added into extracting solution for the dissolubility of medicine ingredient and impurity, alcohol precipitation processing is carried out, by containing alcohol
Liquid stands 75h in 5-10 DEG C, and filtering obtains the second extract；Merge the first extract, the second extract, in 50 DEG C of dryings
50min obtains Chinese medical extract；
(4) by the Chinese medical extract and 10 grams of chitosans, 8 grams of vitamin E powders and by following probiotic group at benefit
Raw bacteria powder：Bacillus subtilis 3,000,000,000/gram, lactobacillus acidophilus 300,000,000/gram, bacillus licheniformis 3,200,000,000/gram, Candida utilis ferment
Female bacterium 500,000,000/gram, mixing treatment is carried out, it is 1.0 × 10 to make the viable bacteria amount in system9Cfu/g obtains active peptide.
Embodiment 4
A kind of preparation method of the active peptide of enhancing milk cow immunity.The preparation method, includes the following steps：
(1) 350 grams of brassicae chinensis,semens, 700 grams of dandelions, 180 grams of Radix Astragalis, 150 grams of Snakegourd Fruits, 120 grams of Chinese violets carry for 2 times
It takes, uses the injection stage water of 10 times of Chinese medicine weight for the first time, after 30 DEG C carry out immersion treatment 30min, heated and decocted using steam
Extraction 1.5h is boiled, second of the injection stage water for using 8 times of Chinese medicine weight decocts extraction after 30 DEG C carry out immersion treatment 30min
1.0h merges extracting solution, obtains the first extracting solution；
(2) 120 grams of river glutinous rehmannias, 120 grams of kings are not stayed, 100 grams of Radix Angelicae Sinensis, 120 grams of honeysuckles, 180 grams of cordate houttuynias carry for 3 times
It takes, uses the injection stage water of 10 times of Chinese medicine weight for the first time, after 35 DEG C carry out immersion treatment 1.5h, heated and decocted using steam
2h is extracted, extracting solution is collected, second of the injection stage water for using 8 times of Chinese medicine weight is decocted after 35 DEG C carry out immersion treatment 1.5h
Extraction 1h is boiled, extracting solution is collected, uses the injection stage water of 6 times of Chinese medicine weight for the third time, after 35 DEG C carry out immersion treatment 1.5h,
1h is extracted, merges extracting solution, obtains the second extracting solution；
(3) after being 1.25 by first extracting solution progress concentration to relative density, concentrate is obtained, to concentrate
The middle ethyl alcohol that 1 times of volume of concentrate is added, carries out alcohol precipitation processing, by containing alcohol liquid in 5-10 DEG C, stands 60h, filtering obtains the
One extract；Second extracting solution oxalic acid (concentration 10%) or sodium bicarbonate (concentration 7%), it is 5.5 to adjust pH, in changing wherein
The ethyl alcohol of 1.5 times of volumes of extracting solution is added into extracting solution for the dissolubility of medicine ingredient and impurity, alcohol precipitation processing is carried out, by containing alcohol
Liquid stands 75h in 5-10 DEG C, and filtering obtains the second extract；Merge the first extract, the second extract, in 50 DEG C of dryings
60min obtains Chinese medical extract；
(4) by the Chinese medical extract and 17 grams of chitosans, 13 grams of vitamin E powders and by following probiotic group at benefit
Raw bacteria powder：Bacillus subtilis 2,300,000,000/gram, lactobacillus acidophilus 300,000,000/gram, bacillus licheniformis 3,200,000,000/gram, Candida utilis ferment
Female bacterium 300,000,000/gram, mixing treatment is carried out, it is 1.0 × 10 to make the viable bacteria amount in system8Cfu/g obtains active peptide.
In order to better illustrate the characteristic of active peptide provided in an embodiment of the present invention, the activity that below prepares embodiment 4
Peptide mixes in feed, and carries out feeding to illness milk cow.Choose cow in milk 246, wherein suffer from after testing mammitis and present+
++ the cow in milk of the above degree 37, to 37 illness Cow-feeding active peptides (500g/ heads/day), continuous feeding 10 days, milk
Middle somatic number and milk cow rehabilitation quantity such as 1 result of table are shown.In addition, in order to avoid with prevent other milk cow illness rates increasing
Add, to the active peptide in cows feeding 100g/ heads/day, continuous feeding 10 days effectively prevent the generation of mammitis, hence it is evident that reduce
It is newly-increased to present ++++degree and +++ the quantity of the illness milk cow of degree.
Table 1
It can be obtained by data above, illness cow in milk rehabilitation rate of the feeding added with activated peptide forage is very high, can reach 97%,
Mastitis for milk cows incidence is substantially reduced, and the body cell quantity in milk significantly reduces, in addition, the output of milk is significantly improved.It is real
The active peptide and the active peptide in embodiment 4 for applying the enhancing milk cow immunity being prepared in a 1-3 have comparable effect.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modification, equivalent replacement or improvement etc., should all be included in the protection scope of the present invention made by within refreshing and principle.
Claims (8)
1. a kind of preparation method of the active peptide of enhancing milk cow immunity, it is characterised in that：Include the following steps：
(1) by 300-500 parts of brassicae chinensis,semens, 500-1000 portions of dandelions, 100-200 parts of Radix Astragalis, 100-150 parts of Snakegourd Fruits, 100-150
Part Chinese violet, first carries out immersion treatment with warm water, then carry out decoction processing, obtains the first extracting solution；
(2) 100-150 parts of river glutinous rehmannias, 100-150 parts of kings are not stayed, 80-150 parts of Radix Angelicae Sinensis, 80-200 portions of honeysuckles, 100-300 parts
Cordate houttuynia first carries out immersion treatment with warm water, then carries out decoction processing, obtains the second extracting solution；
(3) after first extracting solution being carried out concentration, concentrate is obtained, alcohol precipitation processing is carried out, obtains the first extract；
After second extracting solution pH is adjusted to 5.5-6.5, alcohol precipitation processing is carried out, the second extract is obtained；Merge the first extract, second carry
Object is taken, through being dried, obtains Chinese medical extract；
(4) Chinese medical extract and 10-20 parts of chitosans, 8-15 parts of vitamin E powders and probiotics are subjected to mixing treatment,
It is 1.0 × 10 to make the viable bacteria amount in system6-1.0×109Cfu/g obtains active peptide.
2. the preparation method of the active peptide of enhancing milk cow immunity as described in claim 1, it is characterised in that：The probiotics
For probiotics powder agent, wherein including：Bacillus subtilis 20-30 hundred million/gram, lactobacillus acidophilus 1-3 hundred million/gram, bacillus licheniformis
25-32 hundred million/gram, candida utili bacterium 3-5 hundred million/gram.
3. the preparation method of the active peptide of enhancing milk cow immunity as described in claim 1, it is characterised in that：In step (1),
The immersion treatment is 2 times, and the water of 9-11 times of Chinese medicine weight, second of the water for using 7-9 times of Chinese medicine weight is used to soak twice for the first time
It is 30-37 DEG C to steep treatment temperature, and the time is 25-35min；In step (2), the immersion treatment is 3 times, in using for the first time
The water of 9-11 times of medicine weight, second of the water for using 7-9 times of Chinese medicine weight, uses the water of 5-7 times of Chinese medicine weight, impregnates three times for the third time
Treatment temperature is 30-37 DEG C, and the time is for 1.0-1.5h.
4. the preparation method of the active peptide of enhancing milk cow immunity as claimed in claim 3, it is characterised in that：In step (1),
The decoction processing is 2 times, and time first time is 1.2-1.8h, and second of time is 0.8-1.2h；It is described to decoct in step (2)
It is 3 times to boil processing, and time first time is 1.5-2.5h, and second of time is 0.8-1.2h, and the third time time is 0.8-1.2h.
5. the preparation method of the active peptide of enhancing milk cow immunity as described in claim 1, it is characterised in that：At the concentration
The first extracting solution relative density is 1.2-1.25 after reason.
6. the preparation method of the active peptide of enhancing milk cow immunity as described in claim 1, it is characterised in that：At the alcohol precipitation
In reason, the ethyl alcohol of its 1-4 times of volume is added into concentrate or extracting solution.
7. the preparation method of the active peptide of enhancing milk cow immunity as claimed in claim 6, it is characterised in that：At the alcohol precipitation
In reason, by containing alcohol liquid in 5-10 DEG C, 60-75h is stood.
8. the preparation method of the active peptide such as claim 1-7 any one of them enhancing milk cow immunity, it is characterised in that：Step
Suddenly in (1), (2), the warm water used in the immersion treatment is heated up to obtain by injection stage with water.
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| CN118186038A (en) * | 2024-02-07 | 2024-06-14 | 北京伍冠肽斗生物科技有限责任公司 | Dandelion compound peptide, preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104324111A (en) * | 2014-10-10 | 2015-02-04 | 青岛嘉瑞生物技术有限公司 | Chinese herbal medicine feed additive for preventing dairy cow postpartum mastitis |
| CN104491203A (en) * | 2014-12-11 | 2015-04-08 | 郑州后羿制药有限公司 | Chinese herbal medicine fermentative extract liquid for preventing and treating cow mastitis and preparation method of Chinese herbal medicine fermentative extract liquid |
| CN104800421A (en) * | 2015-04-23 | 2015-07-29 | 河南省首家中兽医药服务有限公司 | Traditional Chinese medicine powder for preventing and treating cow subclinical mastitis |
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2018
- 2018-06-27 CN CN201810680422.6A patent/CN108743739A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104324111A (en) * | 2014-10-10 | 2015-02-04 | 青岛嘉瑞生物技术有限公司 | Chinese herbal medicine feed additive for preventing dairy cow postpartum mastitis |
| CN104491203A (en) * | 2014-12-11 | 2015-04-08 | 郑州后羿制药有限公司 | Chinese herbal medicine fermentative extract liquid for preventing and treating cow mastitis and preparation method of Chinese herbal medicine fermentative extract liquid |
| CN104800421A (en) * | 2015-04-23 | 2015-07-29 | 河南省首家中兽医药服务有限公司 | Traditional Chinese medicine powder for preventing and treating cow subclinical mastitis |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118186038A (en) * | 2024-02-07 | 2024-06-14 | 北京伍冠肽斗生物科技有限责任公司 | Dandelion compound peptide, preparation method and application thereof |
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Application publication date: 20181106 |