CN104313104B - Ionic protein peptide and preparation method and application thereof - Google Patents
Ionic protein peptide and preparation method and application thereof Download PDFInfo
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 17
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- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 239000002994 raw material Substances 0.000 claims abstract description 16
- 150000002500 ions Chemical class 0.000 claims abstract description 14
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- 240000008042 Zea mays Species 0.000 claims abstract description 7
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- 230000000091 immunopotentiator Effects 0.000 claims abstract description 7
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- 239000004575 stone Substances 0.000 claims abstract description 7
- 238000000855 fermentation Methods 0.000 claims description 30
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- 235000019743 Choline chloride Nutrition 0.000 claims description 5
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
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- 239000004115 Sodium Silicate Substances 0.000 claims description 5
- 238000010564 aerobic fermentation Methods 0.000 claims description 5
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- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims 1
- 229910052796 boron Inorganic materials 0.000 claims 1
- 230000002708 enhancing effect Effects 0.000 claims 1
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- BXMVKQIIJSXIBU-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) diphenyl phosphate Chemical compound O=C1CCC(=O)N1OP(=O)(OC=1C=CC=CC=1)OC1=CC=CC=C1 BXMVKQIIJSXIBU-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
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- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses an ionic protein peptide which is prepared by fermenting an immunopotentiation solution and a starter with a protein raw material as a carrier. The invention also discloses a preparation method and an application of the above ionic protein peptide. The invention also discloses a special biological feed for growing and fattening pigs on a large-scale pig farm. The special biological feed is composed of raw materials, including corn, ionic protein peptide, soybean meal, wheat bran, stone flour, calcium hydrogen phosphate, salt, a premix and the like. Due to addition of the ionic protein peptide, the feed contains a lot of probiotics and ionic protein peptide and also contains an ion immunopotentiator. Thus, intestinal health of pigs can be improved, growth of pigs can be promoted, and immunity and anti-stress capability can be enhanced.
Description
Technical field
The invention belongs to livestock breeding industry, and in particular to a kind of ion-protein peptide and its preparation method and application.
Background technology
High-quality feed contg. animal protein, its digestibility is high, absorb, good palatability.But Britain occurred in 1997
Rabid ox disease and then betide Holland two evil because of event after, the security of feed contg. animal protein falls under suspicion.At present I
Country be still barred from the animal-based protein matter feed of import foreign countries most area, such as meat meal tankage, chicken meal feed is former
Material.Therefore, people make great efforts to seek that other resources of feed contg. animal protein can be substituted.
Vegetable protein feed can partly substitute animal protein source by deep processing, such as FSPC, big
Beans separate albumen etc., but due to these deep processed products on the one hand due to processing cost the problems such as, the market price is expensive；It is another
Aspect, because the palatability of FSPC is poor, influences the feed intake of piglet, so as to limit such product adding in feed
Dosage.
Functional protein raw material is that cub, poult and part aquatic livestock well grow necessary raw material.I
State's functional feed protein matter raw material edible at present mainly including the animal blood product such as SDPP, globulin powder,
The soybean further process product such as the dairy products such as skimmed milk power, whole milk powder and FSPC, these product prices are high.Dregs of beans
As large quality plant protein feed, contain substantial amounts of ANFs, such as soybean agglutinin, protease inhibitor.
These protide ANFs can reduce utilization rate of the animal to nutrient in dregs of beans, and its anti-nutrition is made for piglet
With more notable, addition crosses conference and causes grice diarrhoea, influences the speed of growth of piglet.Therefore, dregs of beans is limited in piglet
Addition in daily ration.Microbe Fermented Soybean Meal is presently considered to be one kind that is green and efficiently reducing dregs of beans ANFs content
Containing abundant probiotics, Soybean Peptide, organic acid isoreactivity composition in effective way, and fermented product, in livestock and poultry cultivation
The effects such as raising livestock and poultry production performance being played, improved carcass quality, improved immunity of livestock, is a kind of functional protein source
Feed, is widely used in Production of Livestock and Poultry at present.
The content of the invention
Goal of the invention：In order to overcome at present during large-scale pig farm uses fermented feed, operation link is more, easily dirty
The shortcoming that dye miscellaneous bacteria, fermentation efficiency are low, leavening consumption is big, quality is difficult to ensure.The present invention by add ion immune-enhancing effect liquid and
A kind of leavening, there is provided preparation method of ion-protein peptide, and be applied in animal and fowl fodder.
Technical scheme：In order to solve the above-mentioned technical problem, the invention provides a kind of ion-protein peptide, the ion-protein
Peptide is formed by ion immune-enhancing effect liquid and leavening by carrier fermentation of protein raw materials.
Wherein, above-mentioned ion immune-enhancing effect liquid is made up of following raw material by weight：Sodium metasilicate 30-60 parts, it is thio
Sodium sulphate 0.1-0.5 parts, reduced sugar 20-30 parts, sodium chloride 0.1-0.2 parts, borax 0.1-0.2 parts, distilled water 30-50 parts.
Wherein, above-mentioned leavening includes bacillus subtilis, the aerobic bacteria of brewer's yeast composition and Lactobacillus plantarum group
Into anaerobic bacteria.
Wherein, the preparation method of above-mentioned ion-protein peptide, comprises the following steps：
1）100 kilograms of protein raw materials are added 10 ~ 20 kilograms of 10-100 that add water of ion immune-enhancing effect liquid by first step aerobic fermentation
Kilogram, plus 20 ~ 200 grams of aerobic bacteria stirs, and loads common woven bag and ferments 2 ~ 6 days；
2）Second step anaerobic fermentation, mixer is poured into by the material that the first step is fermented, and add water 10 ~ 50 kilograms, plus anaerobism
20 ~ 200 grams of bacterium, after being sufficiently stirred for, loads fermentation in the fermentation bag with check valve, and fermentation obtains ion-protein peptide in two days；
3）Ion-protein peptide after anaerobic fermentation is dried and is obtained final product naturally.
Application of the above-mentioned ion-protein peptide in terms of biological feedstuff is prepared.
A kind of fattening pannage for adding ion-protein peptide, the feed includes the raw material of following ratio of weight and number：It is beautiful
60 ~ 70 parts of ground rice, 2 ~ 10 parts of ion-protein peptide, 15 ~ 20 parts of dregs of beans, 5 ~ 10 parts of wheat bran, 1 ~ 2 part of stone flour, calcium monohydrogen phosphate 0.5 ~ 1.5
Part, 0.3 ~ 0.4 part of salt, 1 part of premix, 0.1 part of Choline Chloride.
Further, the premix is offer in every kilogram of diet:VA 5000 IU；VD3 600 ~800IU；VE
25mg；VK32.5 mg；VB11 mg；VB2 2.5~5mg；VB61~1.5mg；VB120.02mg；The mg of nicotinic acid 15；7 ~ 10mg of pantothenic acid；
The mg of biotin 0.05；The mg of folic acid 0.5；Lys 120 mg；Cu 8~12mg；Mn 20~30mg；Fe 50 mg； Zn 50 mg；I
0.35mg；Se 0.3mg.
During the ion-protein peptide added in pig feed produces this swine rationses preparation technique containing ion-protein peptide, ion
Protein peptides be that fermentation process is crossed or fermentation process after air-dry.Such as daily ration is carried out using the air dried condition of ion-protein peptide to match somebody with somebody
Close, daylight drying or shady place natural air drying should be kept away below 55 DEG C.
Beneficial effect：Present invention employs above-mentioned technical proposal, to delivering for sale when pig is fattened with corn flour, fermentation ion
Based on the concentrated feeds such as protein peptides, dregs of beans, wheat bran, while with the addition of the premix containing vitamin and mineral matter necessary to pig growth
Material, contains substantial amounts of beneficial bacterium, ion polypeptide, immunopotentiator in feed.Due to adding ion-protein peptide in daily ration, not only make
Contain substantial amounts of probiotic in feed, ion polypeptide also contains immunopotentiator, improves pig intestinal health, while pig can be promoted
Growth, strengthen immunity and anti-stress ability, improve food conversion ratio.
Specific embodiment
With reference to embodiment, the present invention will be further described.
Embodiment 1：
Ion-protein peptide makes：
100 kilograms of dregs of beans are added 10 kilograms of ion immune-enhancing effect liquid to add water 10 kilograms and add aerobic bacteria by first step aerobic fermentation
20 grams of bacillus subtilis and brewer's yeast stir, and load common woven bag two days summers of fermentation, six days winters.
Wherein, ionic immunopotentiator is made up of following raw material by weight：30 parts of sodium metasilicate, sodium thiosulfate
0.1 part, 20 parts of reduced sugar, 0.1 part of sodium chloride, 0.1 part of borax, 30 parts of distilled water；
Second step anaerobic fermentation, mixer is poured into by the material that the first step is fermented.Add water 10 kilograms, plus anaerobic bacteria plant
20 grams of lactobacillus.After being sufficiently stirred for, load fermentation in the fermentation bag with check valve, ferment two days.
Ion-protein peptide after anaerobic fermentation is dried naturally.
Growing and fattening pigs biological feedstuff includes the component of following parts by weight：
60 parts of corn flour, 2 parts of ion-protein peptide, 20 parts of dregs of beans, 5 parts of wheat bran, 1.2 parts of stone flour, 0.8 part of calcium monohydrogen phosphate, food
0.3 part of salt, 1 part of premix, [50%] 0.1 part of Choline Chloride；, the premix is offer in every kilogram of diet:VA 5000
IU；VD3 800 IU；VE 25mg；VK3 2.5 mg；VB11 mg；VB2 2.5mg；VB6 1.5mg；VB12 0.02mg；Nicotinic acid 15
mg；The mg of pantothenic acid 10；The mg of biotin 0.05；The mg of folic acid 0.5；Lys 120g；Cu 12mg；Mn 20mg；Fe 50 mg；
Zn 50 mg；I 0.35mg；Se 0.3mg.
Above-mentioned each component is mixed in proportion, conventional method is fed.
Embodiment 2：
Ion-protein peptide makes：
100 kilograms of dregs of beans are added 15 kilograms of ion immune-enhancing effect liquid to add water 40 kilograms, plus aerobic bacteria by first step aerobic fermentation
50 grams of bacillus subtilis and brewer's yeast stir, and load common woven bag two days summers of fermentation, six days winters；
Wherein, ionic immunopotentiator is made up of following raw material by weight：60 parts of sodium metasilicate, sodium thiosulfate
0.5 part, 30 parts of reduced sugar, 0.2 part of sodium chloride, 0.2 part of borax, 50 parts of distilled water；
Second step anaerobic fermentation, mixer is poured into by the material that the first step is fermented.Add water 30 kilograms, plus anaerobic bacteria plant
100 grams of lactobacillus.After being sufficiently stirred for, load fermentation in the fermentation bag with check valve, ferment two days；
Ion-protein peptide after anaerobic fermentation is dried naturally.
Growing and fattening pigs biological feedstuff includes the component of following parts by weight：
70 parts of corn flour, 6 parts of ion-protein peptide, 16 parts of dregs of beans, 8 parts of wheat bran, 1 part of stone flour, 1.5 parts of calcium monohydrogen phosphate, salt
0.4 part, 1 part of premix, [50%] 0.1 part of Choline Chloride；The premix is offer in every kilogram of diet:VA 5000 IU；
VD3 800 IU；VE 25mg；VK3 2.5 mg；VB11 mg；VB2 2.5mg；VB6 1.5mg；VB12 0.02mg；The mg of nicotinic acid 15；
The mg of pantothenic acid 10；The mg of biotin 0.05；The mg of folic acid 0.5；Lys 120g；Cu 12mg；Mn 20mg；Fe 50 mg； Zn
50 mg；I 0.35mg；Se 0.3mg.
Above-mentioned each component is mixed in proportion, conventional method is fed.
Embodiment 3：
Ion-protein peptide makes：
100 kilograms of dregs of beans are added 20 kilograms of ion immune-enhancing effect liquid to add water 100 kilograms by first step aerobic fermentation, plus aerobic
200 grams of bacterium bacillus subtilis and brewer's yeast stir, and load common woven bag two days summers of fermentation, six days winters；Its
In, ionic immunopotentiator is made up of following raw material by weight：45 parts of sodium metasilicate, 0.3 part of sodium thiosulfate, reduction
25 parts of sugar, 0.15 part of sodium chloride, 0.15 part of borax, 40 parts of distilled water；
Second step anaerobic fermentation, mixer is poured into by the material that the first step is fermented.Add water 50 kilograms, plus anaerobic bacteria plant
200 grams of lactobacillus.After being sufficiently stirred for, load fermentation in the fermentation bag with check valve, ferment two days；
Ion-protein peptide after anaerobic fermentation is dried naturally.
Growing and fattening pigs biological feedstuff includes the component of following parts by weight：
65 parts of corn flour, 10 parts of ion-protein peptide, 15 parts of dregs of beans, 10 parts of wheat bran, 2 parts of stone flour, 0.5 part of calcium monohydrogen phosphate, food
0.3 part of salt, 1 part of premix, [50%] 0.1 part of Choline Chloride；, the premix is offer in every kilogram of diet:VA 5000
IU；VD3 800 IU；VE 25mg；VK3 2.5 mg；VB11 mg；VB2 2.5mg；VB6 1.5mg；VB12 0.02mg；Nicotinic acid 15
mg；The mg of pantothenic acid 10；The mg of biotin 0.05；The mg of folic acid 0.5；Lys 120g；Cu 12mg；Mn 20mg；Fe 50 mg；
Zn 50 mg；I 0.35mg；Se 0.3mg.
Above-mentioned each component is mixed in proportion, conventional method is fed.
Test example：The growing and fattening pigs biological feedstuff feeding experiment of embodiment 2.
1st, material and method
1.1 experimental animals and method：DLY three way cross, age in days is close, the later stage of body weight 70kg or so fattens the stage
Fattening pig 48, is randomly divided into 2 treatment, each 3 repetition for the treatment of, and each repeats 8.Control group fed Basic drawing, raw material
Composition：70 parts of corn flour, 22 parts of dregs of beans, 8 parts of wheat bran, 1 part of stone flour, 1.5 parts of calcium monohydrogen phosphate, 0.4 part of salt, 1 part of premix, chlorine
Change [50%] 0.1 part of choline.Test group substitutes 6% dregs of beans with ion-protein peptide, and other compositions content is constant.
1.2 feedings and managements：Experimental period 40d.Whole feeding process is fed by same poultry raiser, Manual material feeding, free choice feeding,
Free water, each group gives identical feeding and management and environmental condition.
1.3 observation indexs：In on-test with the end of, early morning weighs on an empty stomach.At the end of feeding experiment, selected at random per column
2 pigs are butchered.Influence of the observation ion-protein peptide to indexs such as Growth of Pigs performance, feed intake and carcass traits.
1.4 result of the tests
Influence of the ion-protein peptide of table 1 to Growth of Pigs performance
| Project | Average daily gain（kg/d） | Average daily gain（g/d） | Feed-weight ratio |
| Control group | 2.24 | 762.63 | 2.94 |
| Test group | 2.38 | 876.42 | 2.71 |
Test group pig average daily gain improves 6.25% (P compared with control group>0.05), average daily gain is improved compared with control group
14.92%(P<0.05), feed-weight ratio declines 6.8% (P>0.05).
Influence of the ion-protein peptide of table 2 to fattening pig Slaughter
| Project | Dressing percentage/% | The thickness of backfat/cm | Eye muscle area/cm2 |
| Control group | 66.32 | 12.18 | 24.58 |
| Test group | 67.61 | 11.78 | 26.84 |
Ion-protein peptide fattens dressing percentage of hog, eye muscle area to improve, and reduces the thickness of backfat, but the not notable (P of difference
>0.05)。
Influence of the ion-protein peptide of table 3 to fattening pork mass
| Project | pH45min | pH24h | Color Score |
| Control group | 6.30 | 5.46 | 2.40 |
| Test group | 6.42 | 5.50 | 2.59 |
Compared with control group, ion-protein peptide significantly improves growing and fattening pigs pork 45min, 24h longissimus dorsi muscle pH, significantly carries
Growing and fattening pigs pork color scoring (P high>0.05).
Illustrated by above test example, the ion-protein peptide that the present invention is provided can significantly improve daily gain in pigs, pork
45min, 24h longissimus dorsi muscle pH and Color Score, have promotion feeding, improve the price of deed, reduce the thickness of backfat, improve eye muscle area
With the trend of dressing percentage.In general, the ion-protein peptide that the present invention is provided can improve growing and fattening pigs weightening and improve meat.
Claims (1)
1. a kind of fattening pannage of ion-protein peptide, it is characterised in that：The feed includes the original of following ratio of weight and number
Material：60 ~ 70 parts of corn flour, 2 ~ 10 parts of ion-protein peptide, 15 ~ 20 parts of dregs of beans, 5 ~ 10 parts of wheat bran, 1 ~ 2 part of stone flour, calcium monohydrogen phosphate
0.5 ~ 1.5 part, 0.3 ~ 0.4 part of salt, 1 part of premix, 0.1 part of Choline Chloride；The ion-protein peptide is immunized by ionic
Enhancing liquid and leavening are fermented with protein raw materials as carrier, the ionic immunopotentiator be by weight by with
Lower raw material composition：Sodium metasilicate 30-60 parts, sodium thiosulfate 0.1-0.5 parts, reduced sugar 20-30 parts, sodium chloride 0.1-0.2 parts, boron
Sand 0.1-0.2 parts, distilled water 30-50 parts, the leavening include bacillus subtilis, brewer's yeast constitute aerobic bacteria and
The anaerobic bacteria of Lactobacillus plantarum composition；The premix is offer in every kilogram of diet:VA 5000 IU；VD3 600 ~800IU；
VE 25mg；VK32.5 mg；VB11 mg；VB2 2.5~5mg；VB61~1.5mg；VB120.02mg；The mg of nicotinic acid 15；Pantothenic acid 7 ~
10mg；The mg of biotin 0.05；The mg of folic acid 0.5；Lys 120 mg；Cu 8~12mg；Mn 20~30mg；Fe 50 mg； Zn 50
mg；I 0.35mg；Se 0.3mg；
Wherein, the preparation method of the ion-protein peptide is comprised the following steps：1）First step aerobic fermentation, by 100 kilograms of albumen
Raw material adds 10 ~ 20 kilograms of ion immune-enhancing effect liquid to add water 10-100 kilograms, plus 20 ~ 200 grams of aerobic bacteria stirs, and loads common
Woven bag ferments 2 ~ 6 days；2）Second step anaerobic fermentation, mixer is poured into by the material that the first step is fermented, and add water 10 ~ 50 public affairs
Jin, plus 20 ~ 200 grams of anaerobic bacteria, after being sufficiently stirred for, load fermentation in the fermentation bag with check valve, and fermentation obtains ion in two days
Protein peptides；3）Ion-protein peptide after anaerobic fermentation is dried and is obtained final product naturally.
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| CN107801862A (en) * | 2017-11-17 | 2018-03-16 | 河南牧翔动物药业有限公司 | A kind of method of bio-resonance catalystic, fermentative turmeric |
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| CN108850614A (en) * | 2018-08-06 | 2018-11-23 | 杭州光启医疗科技发展有限公司 | A kind of disease-resistant fattening pannage of health care |
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| KR100331952B1 (en) * | 2000-11-23 | 2002-04-09 | 최수일 | The Composition Of Multipurpose High-Functional Alkali Solution, Preparation Thereof, And For The Use Of Nonspecific Immunostimulator |
| ATE400189T1 (en) * | 2001-03-23 | 2008-07-15 | Advanced Bionutrition Corp | DELIVERY OF DISEASE CONTROL AGENTS IN AQUACULTURE USING YEAST CONTAINING BIOACTIVE PROTEINS |
| CN101904432B (en) * | 2009-06-05 | 2013-01-16 | 哈尔滨青禾科技有限公司 | Feed for enhancing disease resistance of piglets and accelerating growth of piglets and preparation method thereof |
| CN101642187B (en) * | 2009-09-02 | 2012-05-30 | 张培举 | Protein feed rich in small peptide and multienzyme thallus and preparation method thereof |
| CN102239959B (en) * | 2010-05-11 | 2013-05-15 | 姜明一 | Preparation method of ion activation liquid |
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| CN107801862A (en) * | 2017-11-17 | 2018-03-16 | 河南牧翔动物药业有限公司 | A kind of method of bio-resonance catalystic, fermentative turmeric |
| CN107801862B (en) * | 2017-11-17 | 2021-07-02 | 河南牧翔动物药业有限公司 | Method for catalytically fermenting turmeric through biological resonance |
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Inventor after: Wang Shanfeng Inventor after: Zou Xinhua Inventor after: Luo Guilan Inventor after: Xing Jun Inventor after: Chen Jun Inventor after: Ma Guofu Inventor after: Chen Chao Inventor before: Wang Shanfeng Inventor before: Luo Guilan Inventor before: Xing Jun Inventor before: Chen Jun Inventor before: Ma Guofu Inventor before: Chen Chao |
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Effective date of registration: 20180103 Address after: 341000 Yingbin Road, Ganzhou economic and Technological Development Zone, Jiangxi Province Patentee after: Boon biotechnology Limited by Share Ltd Address before: 212400 Wenchang East Road, Jiangsu, No. 19, Jiangsu Polytechnic College of Agriculture and Forestry,, Zhenjiang Patentee before: Jiangsu Polytechnic College of Agriculture and Forestry |
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Address after: 341000 Yingbin Road, Ganzhou economic and Technological Development Zone, Jiangxi Province Patentee after: Boon Group Co., Ltd Address before: 341000 Yingbin Road, Ganzhou economic and Technological Development Zone, Jiangxi Province Patentee before: BOEN BIOTECHNOLOGY Co.,Ltd. |