CN107114618A - A kind of preparation of Penaeus Vannmei fermented feed and its application method - Google Patents
A kind of preparation of Penaeus Vannmei fermented feed and its application method Download PDFInfo
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- CN107114618A CN107114618A CN201710491781.2A CN201710491781A CN107114618A CN 107114618 A CN107114618 A CN 107114618A CN 201710491781 A CN201710491781 A CN 201710491781A CN 107114618 A CN107114618 A CN 107114618A
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Abstract
The present invention relates to a kind of preparation of Penaeus Vannmei fermented feed and its application method, this method is that bacillus licheniformis, Lactobacillus plantarum, candida utili are expanded into culture respectively, then according to volume ratio 1：1：The ratio of addition brown sugar (2g/1000mL) and running water in 1 mixing, mixed bacteria liquid, strain liquid and water is 1：80, it is well mixed, and prawn mixed feed, according to 2：5 ratio stirs, the 28h of plastic sheeting sealing and fermenting 18, and fermented feed is made.Present invention also offers the application method of the fermented feed, the fermented feed of making is fed into Penaeus Vannmei, once a day, feeding volume is the 20 30% of day total feeding volume.Fermented feed prepared by the present invention, prawn nitric oxide synthase, superoxide dismutase activity, activity of catalase, Phenoloxidase Activities, glutathione peroxidase activity, TAC can be significantly improved, hence it is evident that reduction mda content.It is obviously improved the flora composition of gut of shrimp, hence it is evident that improve prawn culturing survival rate.
Description
Technical field
The invention belongs to feed processing technology field, and in particular to be a kind of Penaeus Vannmei fermented feed preparation and
Its application method.
Background technology
It with microorganism etc. is biological feed fermentation agent strain that fermented feed, which is, and feedstuff is converted into microbial cells egg
In vain, the biological fermentation feed that bioactive micro peptide amino acid, microbial activity probiotics etc. are integrated.
Environment of Litopenaeus vannamei Low is introduced into China from eighties of last century late nineteen eighties, so far the existing history of more than 30 years, with
The progress of science and technology, cultivation density and intensive degree more and more higher, the yield of aquatic products is on the one hand improved, promotes
The development of aquaculture；On the other hand the Tough questions that can not be ignored also are brought --- the deterioration of breeding environment, add
The degeneration of prawn elite germplasm, causes neurological susceptibility increase of the shrimp body to disease, and disease is grown, it has also become restriction shrimp farming development
Maximum bottleneck.
In order to control the disease wreaked havoc, antibiotic is widely used, although originally achieving obvious effect, long
Phase, immoderate abuse of antibiotics to animal in itself, cultured product and environment bring a series of problem.Breeding resistant variety
Disease-resistant method with modification inhereditary material is also extremely immature, along with prawn animal forms solely during long-term life concern
Some immune systems, mainly with nonSing ularity it is immune based on, such raising prawn autoimmunity and to communicable disease resistance
Active demand makes people that sight more to have been invested to the development of novel fodder.Experimental results demonstrate fermented feed is in animal diseases
Effect in preventing and treating is notable, and because its safety and environmental friendliness is widely used in culture fishery.
By retrieval, following 2 patent documents related to present patent application are found：1st, the invention discloses one kind
Penaeus Vannmei microbiological feed addictive special and preparation method thereof (CN201510726597.2), belongs to microbe additive
Field.Its preparation method is：(1) by corn flour, maltodextrin, seawood meal disinfection by ultraviolet light 10s；(2) by corn flour, soybean
Powder, maltodextrin and seawood meal are well mixed, add water and stir to pasty state, be gradually heated to 80 DEG C, add carragheen, continue to stir
Mix, be incubated simultaneously, obtain mixing paste mixing；(3) casein sodium, edible salt, vitamin are added into the mixing paste mixing of step (2)
C, black nightshade fruit juice, disodium succinate and fishbone dust, then add bacillus and nitrobacteria；5min is stirred, mixture is squeezed
Into graininess, dry.2nd, the invention discloses a kind of Environment of Litopenaeus vannamei Low fermented feed and its production method
(CN201611252298.0), comprise the technical steps that：(1) defatted rice bran is first subjected to ultramicro grinding in micronizer
Reach 80 mesh fineness；(2) by defatted rice bran made from brown sugar, Saccharomyces cerevisiae, EM stostes, only son's acid glyceride and step (1)
It is well mixed by weight percentage；(3) mixture made from step (2) is transferred in the environment of sealing anaerobic, in 20-37 DEG C of bar
Ferment 20~30h under part.By comparing, the technical scheme and extraction object of patent document disclosed above have with present patent application
The difference of essence, present patent application has novelty.
The content of the invention
The purpose of the present invention be in view of the shortcomings of the prior art, and propose a kind of Penaeus Vannmei fermented feed preparation and
Its application method.
The present invention solves its technical problem and takes following technical scheme to realize：
A kind of preparation method of Penaeus Vannmei fermented feed, including step are as follows：
(1) bacillus licheniformis, Lactobacillus plantarum and candida utili seed culture fluid are prepared；
(2) bacillus licheniformis, Lactobacillus plantarum and candida utili seed culture fluid are inoculated into culture medium respectively
In, expand culture, then according to volume ratio 1：1：1 mixing；
(3) add running water according to Jun Zhong Ye ﹕ water for 1 ﹕ 80-100 ratio in above-mentioned mixed bacteria liquid to be well mixed, so
Brown sugar is added according to 2g/1000mL concentration afterwards, is then stirred again with prawn mixed feed according to 2 ﹕ 5 ratio, plastics
Diaphragm seal fermentation 18-28h, obtains the fermented feed.
Moreover, bacillus licheniformis culture medium is the agar medium of seawater 2216 in the step (2)；Lactobacillus plantarum is trained
It is MRS culture mediums to support base；It is malt extract medium to produce Ruan's Candida seed culture medium.
Moreover, in the fermentation process of the step (3), temperature control is in the range of 25-33 DEG C.
A kind of application method of Penaeus Vannmei fermented feed as described above, the Penaeus Vannmei fermented feed is thrown
Penaeus Vannmei is fed, once a day, feeding volume is the 20-30% of day total feeding volume.
Advantages and positive effects of the present invention are：
1st, the present invention is the disease during the culture of Penaeus vannamei that sternness is solved the problems, such as by microbial technique, specifically
It is to utilize efficient microorganism, metabolite and its forage degradation active material, into after prawn digestive system, increasing can be bred rapidly
The immunity of strong prawn body, improves the survival rate of cultivated animals, reduces the incidence of disease.
2nd, the beneficial bacteria discharged through gut of shrimp can also be by the animal excrements in breeding water body, residual bait, biological residuum
Carbon dioxide, nitrate, phosphate, sulfate etc. are decomposed into rapidly Deng organic matter, are promoted unicellular alga breeding and are grown, are
Cultivate object and oxygen is provided.Follow this reciprocal, constitute a benign ecological circulation, make cultivated animals bacterium phase mutually reached with algae it is flat
Weighing apparatus, builds good aquatic environment.
Brief description of the drawings
Fig. 1 is impact effect figure of the fermented feed to Penaeus Vannmei survival rate in example 1；
Fig. 2 is impact effect figure of the fermented feed to Penaeus Vannmei survival rate in example 2；
Fig. 3 is impact effect figure of the fermented feed to Penaeus Vannmei survival rate in example 3.
Embodiment
The embodiment of the present invention is further described below：It is emphasized that embodiment of the present invention is explanation
Property, rather than it is limited, therefore the present invention is not limited to the embodiment described in embodiment, it is every by this area
The other embodiment that technical staff's technique according to the invention scheme is drawn, also belongs to the scope of protection of the invention.
Embodiment 1
A kind of preparation of Penaeus Vannmei fermented feed and its application method, comprise the following steps：
(1) bacillus licheniformis, Lactobacillus plantarum, candida utili are expanded into culture respectively, then according to volume ratio
1：1：The ratio of addition brown sugar (2g/1000mL) and running water in 1 mixing, mixed bacteria liquid, strain liquid and water is 1：80, mixing is equal
It is even, and prawn mixed feed, according to 2：5 ratio stirs, plastic sheeting sealing and fermenting 22h, and 28 DEG C of fermentation temperature is made
Fermented feed.
(2) fermented feed of making is fed into Penaeus Vannmei, once a day, feeding volume is the 20- of day total feeding volume
30%, prawn culturing survival rate improves 15.62%, as shown in Figure 1.
Embodiment 2
A kind of preparation of Penaeus Vannmei fermented feed and its application method, comprise the following steps：
(1) bacillus licheniformis, Lactobacillus plantarum, candida utili are expanded into culture respectively, then according to volume ratio
1：1：The ratio of addition brown sugar (2g/1000mL) and running water in 1 mixing, mixed bacteria liquid, strain liquid and water is 1：80, mixing is equal
It is even, and prawn mixed feed, according to 2：5 ratio stirs, plastic sheeting sealing and fermenting 24h, and 30 DEG C of fermentation temperature is made
Fermented feed.
(2) fermented feed of making is fed into Penaeus Vannmei, once a day, feeding volume is the 20- of day total feeding volume
30%, prawn culturing survival rate improves 16.15%, as shown in Figure 2.
Embodiment 3
A kind of preparation of Penaeus Vannmei fermented feed and its application method, comprise the following steps：
(1) bacillus licheniformis, Lactobacillus plantarum, candida utili are expanded into culture respectively, then according to volume ratio
1：1：The ratio of addition brown sugar (2g/1000mL) and running water in 1 mixing, mixed bacteria liquid, strain liquid and water is 1：80, mixing is equal
It is even, and prawn mixed feed, according to 2：5 ratio stirs, plastic sheeting sealing and fermenting 20h, and 30 DEG C of fermentation temperature is made
Fermented feed.
(2) fermented feed of making is fed into Penaeus Vannmei, once a day, feeding volume is the 20- of day total feeding volume
30%, prawn culturing survival rate improves 16.07%, as shown in Figure 3.
Claims (4)
1. a kind of preparation method of Penaeus Vannmei fermented feed, it is characterised in that as follows including step：
(1) bacillus licheniformis, Lactobacillus plantarum and candida utili seed culture fluid are prepared；
(2) bacillus licheniformis, Lactobacillus plantarum and candida utili seed culture fluid are inoculated into culture medium respectively, expanded
Big culture, then according to volume ratio 1：1：1 mixing；
(3) add running water for 1 ﹕ 80-100 ratio according to Jun Zhong Ye ﹕ water in above-mentioned mixed bacteria liquid to be well mixed, then press
Concentration according to 2g/1000mL adds brown sugar, is then stirred again with prawn mixed feed according to 2 ﹕ 5 ratio, plastic sheeting
Sealing and fermenting 18-28h, obtains the fermented feed.
2. the preparation method of Penaeus Vannmei fermented feed according to claim 1, it is characterised in that：The step (2)
Middle bacillus licheniformis culture medium is the agar medium of seawater 2216；Lactobacillus plantarum culture medium is MRS culture mediums；Produce the false silk of Ruan
Yeast starter culture medium is malt extract medium.
3. the preparation method of Penaeus Vannmei fermented feed according to claim 1, it is characterised in that：The step (3)
Fermentation process in, temperature control is in the range of 25-33 DEG C.
4. a kind of application method of Penaeus Vannmei fermented feed as described in power 1, it is characterised in that：By the Penaeus Vannmei
Fermented feed feeds Penaeus Vannmei, once a day, and feeding volume is the 20-30% of day total feeding volume.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108271952A (en) * | 2018-03-07 | 2018-07-13 | 浙江汇丰世邦生物工程有限公司 | A kind of fish ferment and the preparation method and application thereof |
| CN108402336A (en) * | 2018-03-07 | 2018-08-17 | 浙江汇丰世邦生物工程有限公司 | A kind of common eel ferment and the preparation method and application thereof |
| CN108402337A (en) * | 2018-03-07 | 2018-08-17 | 浙江汇丰世邦生物工程有限公司 | One seed shrimp ferment and the preparation method and application thereof |
| CN109619280A (en) * | 2018-12-24 | 2019-04-16 | 天津科技大学 | A kind of Penaeus Vannmei special nutritional health-care agent |
| CN109619324A (en) * | 2018-12-05 | 2019-04-16 | 广西壮族自治区水产科学研究院 | A kind of aquatic feeds predigestion method and products thereof and application |
| CN110915735A (en) * | 2019-11-20 | 2020-03-27 | 广东金阳生物技术有限公司 | Efficient healthy culture method for prawns |
| CN111820337A (en) * | 2020-07-14 | 2020-10-27 | 华南农业大学 | Fermented feed and simple preparation method thereof |
| CN112704167A (en) * | 2019-10-27 | 2021-04-27 | 湛江嘉田科技有限公司 | Special material formula for marking thickness of penaeus vannamei boone seedlings and use method thereof |
| CN114223810A (en) * | 2021-12-13 | 2022-03-25 | 南京宝辉生物饲料有限公司 | Soft pellet feed for freshwater shrimp culture and preparation method thereof |
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| CN100998369A (en) * | 2006-12-27 | 2007-07-18 | 辽宁东亚种业有限公司 | Bo-gen double micro-organism protein forage |
| CN101352197A (en) * | 2007-07-27 | 2009-01-28 | 严明 | Method for producing fermented feed and anaerobic fermentation bag thereof |
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2017
- 2017-06-26 CN CN201710491781.2A patent/CN107114618A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100998369A (en) * | 2006-12-27 | 2007-07-18 | 辽宁东亚种业有限公司 | Bo-gen double micro-organism protein forage |
| CN101352197A (en) * | 2007-07-27 | 2009-01-28 | 严明 | Method for producing fermented feed and anaerobic fermentation bag thereof |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108271952A (en) * | 2018-03-07 | 2018-07-13 | 浙江汇丰世邦生物工程有限公司 | A kind of fish ferment and the preparation method and application thereof |
| CN108402336A (en) * | 2018-03-07 | 2018-08-17 | 浙江汇丰世邦生物工程有限公司 | A kind of common eel ferment and the preparation method and application thereof |
| CN108402337A (en) * | 2018-03-07 | 2018-08-17 | 浙江汇丰世邦生物工程有限公司 | One seed shrimp ferment and the preparation method and application thereof |
| CN109619324A (en) * | 2018-12-05 | 2019-04-16 | 广西壮族自治区水产科学研究院 | A kind of aquatic feeds predigestion method and products thereof and application |
| CN109619280A (en) * | 2018-12-24 | 2019-04-16 | 天津科技大学 | A kind of Penaeus Vannmei special nutritional health-care agent |
| CN112704167A (en) * | 2019-10-27 | 2021-04-27 | 湛江嘉田科技有限公司 | Special material formula for marking thickness of penaeus vannamei boone seedlings and use method thereof |
| CN110915735A (en) * | 2019-11-20 | 2020-03-27 | 广东金阳生物技术有限公司 | Efficient healthy culture method for prawns |
| CN111820337A (en) * | 2020-07-14 | 2020-10-27 | 华南农业大学 | Fermented feed and simple preparation method thereof |
| CN114223810A (en) * | 2021-12-13 | 2022-03-25 | 南京宝辉生物饲料有限公司 | Soft pellet feed for freshwater shrimp culture and preparation method thereof |
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