CN110643523A - Formula and preparation method of fermentation microbial inoculum for fermented feed - Google Patents
Formula and preparation method of fermentation microbial inoculum for fermented feed Download PDFInfo
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Abstract
The invention discloses a formula and a preparation method of a fermentation microbial inoculum for fermented feed, belonging to the technical field of preparation of fermented feed, and comprising the following raw materials in parts by mass: 0.1-4 parts of bacillus subtilis, 0.2-5 parts of saccharomyces cerevisiae, 0.5-3 parts of enterococcus faecalis, 1-8 parts of zeolite powder serving as a carrier, 1-10 parts of lactobacillus acidophilus and 20-50 parts of lactobacillus plantarum, and a proper amount of wheat bran and rice bran are added with purified water according to a ratio of 1:1 and mixed to prepare a culture medium; adding zeolite powder into the culture medium, and uniformly stirring; preparing three culture bottles, wherein the three culture bottles are numbered as 1, 2 and 3 respectively, and respectively filling the three culture bottles with the same amount of culture medium; respectively adding saccharomyces cerevisiae into the three culture bottles; the method comprises the steps of respectively inoculating bacillus subtilis, enterococcus faecalis, lactobacillus acidophilus and lactobacillus plantarum in different proportions into three culture bottles, placing the culture bottles in an incubator for culture, detecting and evaluating fermentation after fermentation is completed, and then sealing and bagging.
Description
Technical Field
The invention relates to the technical field of preparation of fermented feed, in particular to a formula and a preparation method of a fermentation microbial inoculum of fermented feed.
Background
China is a big agricultural country, and in recent years, a plurality of problems are exposed in cultivation, such as large-scale outbreak of various infectious diseases, antibiotic abuse, low feed utilization rate, continuous rising of feed raw material price, rising of cultivation cost and the like. Therefore, how to improve the utilization rate of the feed, improve the quality of cheap protein and fat raw materials, reduce antibiotics even without antibiotics becomes a new problem faced by the modern feed industry. The biological feed is a green, safe and environment-friendly feed. By adopting a microbial fermentation technology, renewable plant resources and various agricultural and sideline product processing wastes are utilized, the feed quality can be improved, the digestibility of the feed is improved, the intestinal microbial balance of animals is maintained, the immunity of the animals is improved, the breeding cost is reduced, and the fermented feed has wide prospects in the application of the feed industry.
However, there are many problems in the development of current fermented feeds: the sources of fermentation strains are various, the product quality is uneven, and the produced fermented feed is unfavorable for the growth of animals.
Disclosure of Invention
The fermented feed produced by the fermentation inoculant prepared by the method stimulates the growth and development of animal immune organs, enhances the cellular immunity and humoral immunity functions, improves the immunity of groups, generates natural antibiotics and is beneficial to the health of organisms; meanwhile, antibacterial substances such as bacteriocin and the like can be generated, the growth of pathogenic bacteria such as escherichia coli and salmonella is inhibited, and the intestinal microenvironment is improved; can also inhibit the reproduction of urease-producing bacteria and putrefying bacteria in the intestinal tract, reduce the content of urease and endotoxin in the intestinal tract and reduce the content of ammonia and endotoxin in blood, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
the formula of the fermentation microbial inoculum for fermenting the feed comprises the following raw materials in parts by mass: 0.1-4 parts of bacillus subtilis, 0.2-5 parts of saccharomyces cerevisiae, 0.5-3 parts of enterococcus faecalis, 1-8 parts of zeolite powder serving as a carrier, 1-10 parts of lactobacillus acidophilus and 20-50 parts of lactobacillus plantarum.
Preferably, the feed comprises the following raw materials in parts by mass: 0.1 part of bacillus subtilis, 0.2 part of saccharomyces cerevisiae, 0.5 part of enterococcus faecalis, 1 part of zeolite powder used as a carrier, 1 part of lactobacillus acidophilus and 50 parts of lactobacillus plantarum.
Preferably, the method comprises the following raw materials: 2 parts of bacillus subtilis, 2.6 parts of saccharomyces cerevisiae, 1.75 parts of enterococcus faecalis, 4.5 parts of zeolite powder serving as a carrier, 5.5 parts of lactobacillus acidophilus and 35 parts of lactobacillus plantarum.
Preferably, the method comprises the following raw materials: 4 parts of bacillus subtilis, 5 parts of saccharomyces cerevisiae, 3 parts of enterococcus faecalis, 8 parts of zeolite powder serving as a carrier, 10 parts of lactobacillus acidophilus and 20 parts of lactobacillus plantarum.
Preferably, the energy feed is a mixture of wheat bran and rice bran in a ratio of 1: 1.
The invention provides another technical scheme as follows: a preparation method of a zymophyte agent for fermenting feed is characterized by comprising the following steps:
the method comprises the following steps: adding a proper amount of wheat bran and rice bran into purified water according to a ratio of 1:1, mixing while adding water, stirring uniformly, and when the mixture is held tightly by hands, finishing the preparation of a culture medium when water seeps out from finger gaps;
step two: adding zeolite powder into the prepared culture medium, and uniformly stirring;
step three: preparing three culture bottles, wherein the three culture bottles are numbered as 1, 2 and 3 respectively, and respectively filling the three culture bottles with the same amount of culture medium;
step four: respectively adding saccharomyces cerevisiae into the three culture bottles;
step five: on a clean bench, bacillus subtilis, enterococcus faecalis, lactobacillus acidophilus and lactobacillus plantarum with different proportions are respectively inoculated into three culture bottles, and the culture bottles are placed in an incubator for culture.
Step six: after fermentation is finished, detection and evaluation of the fermentation are carried out, and then the fermentation is sealed and packaged and stored in a certain dry environment.
Preferably, the amount of the culture medium added in step three is below the 100 ml mark of the culture flask.
Compared with the prior art, the invention has the beneficial effects that:
according to the formula and the preparation method of the fermentation microbial inoculum of the fermented feed, bacillus subtilis, saccharomyces cerevisiae, enterococcus faecalis, zeolite powder serving as a carrier, lactobacillus acidophilus and lactobacillus plantarum are adopted, and compared with the existing fermented feed, the fermented feed produced by the fermentation microbial inoculum prepared by the method has the advantages that the expansion effect of the prepared product feed is good, animals like to eat, the growth and development of animal immune organs can be stimulated, the cellular immunity and humoral immunity functions are enhanced, the group immunity is improved, natural antibiotics are generated, and the health of organisms is facilitated; meanwhile, antibacterial substances such as bacteriocin and the like can be generated, the growth of pathogenic bacteria such as escherichia coli and salmonella is inhibited, and the intestinal microenvironment is improved; can also inhibit the reproduction of urease-producing bacteria and putrefying bacteria in intestinal tract, reduce the content of urease and endotoxin in intestinal tract, reduce the content of ammonia and endotoxin in blood, and is beneficial to the health and growth of animals.
Detailed Description
The following examples will explain the present invention in detail, however, the present invention is not limited thereto. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the embodiment of the invention: the formula of the fermentation microbial inoculum for fermenting the feed comprises the following raw materials in parts by mass: 0.1-4 parts of bacillus subtilis, 0.2-5 parts of saccharomyces cerevisiae, 0.5-3 parts of enterococcus faecalis, 1-8 parts of zeolite powder serving as a carrier, 1-10 parts of lactobacillus acidophilus and 20-50 parts of lactobacillus plantarum.
The first embodiment is as follows:
taking raw materials: 0.1 part of bacillus subtilis, 0.2 part of saccharomyces cerevisiae, 0.5 part of enterococcus faecalis, 1 part of zeolite powder used as a carrier, 1 part of lactobacillus acidophilus and 50 parts of lactobacillus plantarum.
Based on the raw material ratio, the preparation method of the fermentation microbial inoculum for fermenting the feed comprises the following steps:
the method comprises the following steps: adding a proper amount of wheat bran and rice bran into purified water according to a ratio of 1:1, mixing while adding water, stirring uniformly, and when the mixture is held tightly by hands, finishing the preparation of a culture medium when water seeps out from finger gaps;
step two: adding zeolite powder into the prepared culture medium, and uniformly stirring;
step three: preparing 1 culture bottle with the number of 1 for distinguishing and comparison, and filling the culture bottle with the culture medium, wherein the addition amount of the culture medium is below the 100 ml scale mark of the culture bottle, so that the excessive culture medium is avoided;
step four: adding saccharomyces cerevisiae into the culture bottle;
step five: inoculating bacillus subtilis, enterococcus faecalis, lactobacillus acidophilus and lactobacillus plantarum into a culture bottle on a superclean workbench, and placing the culture bottle in an incubator for culture;
step six: after fermentation is finished, detection and evaluation of the fermentation are carried out, and then the fermentation is sealed and packaged and stored in a certain dry environment.
After the fermentation inoculum prepared by the embodiment is prepared into a fermented feed and fed, the health condition and the growth speed of animals are observed and recorded.
Example two:
taking raw materials: 2 parts of bacillus subtilis, 2.6 parts of saccharomyces cerevisiae, 1.75 parts of enterococcus faecalis, 4.5 parts of zeolite powder serving as a carrier, 5.5 parts of lactobacillus acidophilus and 35 parts of lactobacillus plantarum.
Based on the raw material ratio, the preparation method of the fermentation microbial inoculum for fermenting the feed comprises the following steps:
the method comprises the following steps: adding a proper amount of wheat bran and rice bran into purified water according to a ratio of 1:1, mixing while adding water, stirring uniformly, and when the mixture is held tightly by hands, finishing the preparation of a culture medium when water seeps out from finger gaps;
step two: adding zeolite powder into the prepared culture medium, and uniformly stirring;
step three: preparing 1 culture bottle with the number of 2 for distinguishing and comparison, and filling the culture bottle with the culture medium, wherein the addition amount of the culture medium is below the 100 ml scale mark of the culture bottle, so that the excessive culture medium is avoided;
step four: adding saccharomyces cerevisiae into the culture bottle;
step five: inoculating bacillus subtilis, enterococcus faecalis, lactobacillus acidophilus and lactobacillus plantarum into a culture bottle on a superclean workbench, and placing the culture bottle in an incubator for culture;
step six: after fermentation is finished, detection and evaluation of the fermentation are carried out, and then the fermentation is sealed and packaged and stored in a certain dry environment.
After the fermentation inoculum prepared by the embodiment is prepared into a fermented feed and fed, the health condition and the growth speed of animals are observed and recorded.
Example three:
taking raw materials: 4 parts of bacillus subtilis, 5 parts of saccharomyces cerevisiae, 3 parts of enterococcus faecalis, 8 parts of zeolite powder serving as a carrier, 10 parts of lactobacillus acidophilus and 20 parts of lactobacillus plantarum.
Based on the raw material ratio, the preparation method of the fermentation microbial inoculum for fermenting the feed comprises the following steps:
the method comprises the following steps: adding a proper amount of wheat bran and rice bran into purified water according to a ratio of 1:1, mixing while adding water, stirring uniformly, and when the mixture is held tightly by hands, finishing the preparation of a culture medium when water seeps out from finger gaps;
step two: adding zeolite powder into the prepared culture medium, and uniformly stirring;
step three: preparing 1 culture bottle, numbering 3, and loading into the culture medium, wherein the addition amount of the culture medium is below 100 ml of the scale mark of the culture bottle, so as to avoid excessive culture medium;
step four: adding saccharomyces cerevisiae into the culture bottle;
step five: inoculating bacillus subtilis, enterococcus faecalis, lactobacillus acidophilus and lactobacillus plantarum into a culture bottle on a superclean workbench, and placing the culture bottle in an incubator for culture;
step six: after fermentation is finished, detection and evaluation of the fermentation are carried out, and then the fermentation is sealed and packaged and stored in a certain dry environment.
After the fermentation inoculum prepared by the embodiment is prepared into a fermented feed and fed, the health condition and the growth speed of animals are observed and recorded.
After the fermented feed prepared from the fermentation inoculum prepared in the three embodiments is fed to animals, the sleeping and moving conditions of the animals are observed and recorded, and the weight weighing condition is carried out once in half a month, so that the product prepared in the embodiment 1 has the worst animal eating effect, and compared with the products prepared in the embodiments 1 and 3, the product prepared in the embodiment 2 has the best animal eating effect, the animals grow healthily and the weight of the animals is increased quickly. Therefore, the formulation of example 2 is the optimal formulation.
To better demonstrate the comparison with the three examples described above, three comparative examples are provided below for comparison with the examples described above:
comparative example 1: 0.2-5 parts of saccharomyces cerevisiae, 0.5-3 parts of enterococcus faecalis, 1-8 parts of zeolite powder used as a carrier, 1-10 parts of lactobacillus acidophilus and 20-50 parts of lactobacillus plantarum; the preparation method is the same as that of the examples 1-3, so the details are not repeated, and the health condition and the growth speed of animals are observed and recorded after fermented feed is prepared by adopting the zymophyte agent prepared by the comparative example and fed.
Comparative example 2: 0.1-4 parts of bacillus subtilis, 0.2-5 parts of saccharomyces cerevisiae, 1-8 parts of zeolite powder used as a carrier, 1-10 parts of lactobacillus acidophilus and 20-50 parts of lactobacillus plantarum; the preparation method is the same as that of the examples 1-3, so the details are not repeated, and the health condition and the growth speed of animals are observed and recorded after fermented feed is prepared by adopting the zymophyte agent prepared by the comparative example and fed.
Comparative example 3: 0.1-4 parts of bacillus subtilis, 0.5-3 parts of enterococcus faecalis, 1-8 parts of zeolite powder serving as a carrier, 1-10 parts of lactobacillus acidophilus and 20-50 parts of lactobacillus plantarum; the preparation method is the same as that of the examples 1-3, so the details are not repeated, and the health condition and the growth speed of animals are observed and recorded after fermented feed is prepared by adopting the zymophyte agent prepared by the comparative example and fed.
After the fermented feed prepared by the fermented microbial inoculum prepared in the three comparative examples is fed to animals, the sleeping and moving conditions of the animals are observed and recorded, and the weight weighing condition of the animals is measured once in half a month, so that the three examples are compared, the animal immunity of the product prepared by eating the comparative example 1 is relatively poor, the animal health condition of the product prepared by eating the comparative example 2 is poor, the growth is slow, the foaming and fluffing effect of the product prepared by the comparative example 3 is poor, the animal consumption is low, and the animal is not favored to eat.
In summary, the following steps: according to the formula and the preparation method of the fermentation microbial inoculum for the fermented feed, the bacillus subtilis, the saccharomyces cerevisiae, the enterococcus faecalis, the zeolite powder serving as a carrier, the lactobacillus acidophilus and the lactobacillus plantarum are adopted, and the fermented feed produced by the fermentation microbial inoculum prepared by the method is good in expansion effect compared with the existing fermented feed, animals like to eat, can stimulate the growth and development of animal immune organs, enhance the cellular immunity and humoral immunity functions, improve the group immunity, generate natural antibiotics and is beneficial to the health of organisms; meanwhile, antibacterial substances such as bacteriocin and the like can be generated, the growth of pathogenic bacteria such as escherichia coli and salmonella is inhibited, and the intestinal microenvironment is improved; can also inhibit the reproduction of urease-producing bacteria and putrefying bacteria in intestinal tract, reduce the content of urease and endotoxin in intestinal tract, reduce the content of ammonia and endotoxin in blood, and is beneficial to the health and growth of animals.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The formula of the fermentation microbial inoculum for fermenting the feed is characterized by comprising the following raw materials in parts by mass: 0.1-4 parts of bacillus subtilis, 0.2-5 parts of saccharomyces cerevisiae, 0.5-3 parts of enterococcus faecalis, 1-8 parts of zeolite powder serving as a carrier, 1-10 parts of lactobacillus acidophilus and 20-50 parts of lactobacillus plantarum.
2. The formula of the fermentation inoculant for fermented feed according to claim 1, wherein the formula comprises the following raw materials in parts by mass: 0.1 part of bacillus subtilis, 0.2 part of saccharomyces cerevisiae, 0.5 part of enterococcus faecalis, 1 part of zeolite powder used as a carrier, 1 part of lactobacillus acidophilus and 50 parts of lactobacillus plantarum.
3. The formula of the zymophyte agent for fermenting the feed according to claim 1, which is characterized by comprising the following raw materials: 2 parts of bacillus subtilis, 2.6 parts of saccharomyces cerevisiae, 1.75 parts of enterococcus faecalis, 4.5 parts of zeolite powder serving as a carrier, 5.5 parts of lactobacillus acidophilus and 35 parts of lactobacillus plantarum.
4. The formula of the zymophyte agent for fermenting the feed according to claim 1, which is characterized by comprising the following raw materials: 4 parts of bacillus subtilis, 5 parts of saccharomyces cerevisiae, 3 parts of enterococcus faecalis, 8 parts of zeolite powder serving as a carrier, 10 parts of lactobacillus acidophilus and 20 parts of lactobacillus plantarum.
5. The formulation of the fermentation inoculum for fermented feed of claim 1, wherein the energy feed is a mixture of wheat bran and rice bran in a ratio of 1: 1.
6. A method for preparing a fermentation inoculum for fermenting a feed according to any one of claims 1 to 5, comprising the steps of:
the method comprises the following steps: adding a proper amount of wheat bran and rice bran into purified water according to a ratio of 1:1, mixing while adding water, stirring uniformly, and when the mixture is held tightly by hands, finishing the preparation of a culture medium when water seeps out from finger gaps;
step two: adding zeolite powder into the prepared culture medium, and uniformly stirring;
step three: preparing three culture bottles, wherein the three culture bottles are numbered as 1, 2 and 3 respectively, and respectively filling the three culture bottles with the same amount of culture medium;
step four: respectively adding saccharomyces cerevisiae into the three culture bottles;
step five: respectively inoculating bacillus subtilis, enterococcus faecalis, lactobacillus acidophilus and lactobacillus plantarum in different proportions into three culture bottles on a superclean workbench, and placing the culture bottles in an incubator for culture;
step six: after fermentation is finished, detection and evaluation of the fermentation are carried out, and then the fermentation is sealed and packaged and stored in a certain dry environment.
7. The formula of the zymophyte agent for fermented feed according to claim 6, wherein the addition amount of the culture medium in the third step is below the 100 ml scale mark of the culture bottle.
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| CN112753891A (en) * | 2021-01-19 | 2021-05-07 | 广州飞禧特生物科技有限公司 | Novel fermented feed for hybrid raw fish fry and preparation method thereof |
| CN114797524A (en) * | 2022-05-26 | 2022-07-29 | 江苏三智生物科技有限公司 | Liquid combination proportioning device for photosynthetic bacteria and bacillus |
| WO2023187358A1 (en) * | 2022-03-28 | 2023-10-05 | Hel1X Ltd | Non-antibiotic antimicrobial compositions |
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| CN114797524A (en) * | 2022-05-26 | 2022-07-29 | 江苏三智生物科技有限公司 | Liquid combination proportioning device for photosynthetic bacteria and bacillus |
| CN114797524B (en) * | 2022-05-26 | 2023-06-27 | 江苏三智生物科技有限公司 | Liquid combination proportioning device for photosynthetic bacteria and bacillus |
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Application publication date: 20200103 |