CN115005159B - Breeding method for maintaining intestinal health of piglets and application thereof - Google Patents
Breeding method for maintaining intestinal health of piglets and application thereof Download PDFInfo
- Publication number
- CN115005159B CN115005159B CN202210672530.5A CN202210672530A CN115005159B CN 115005159 B CN115005159 B CN 115005159B CN 202210672530 A CN202210672530 A CN 202210672530A CN 115005159 B CN115005159 B CN 115005159B
- Authority
- CN
- China
- Prior art keywords
- parts
- fermentation
- piglets
- powder
- intestinal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000007413 intestinal health Effects 0.000 title claims abstract description 39
- 238000009395 breeding Methods 0.000 title claims abstract description 28
- 238000000855 fermentation Methods 0.000 claims abstract description 80
- 230000004151 fermentation Effects 0.000 claims abstract description 72
- 150000001875 compounds Chemical class 0.000 claims abstract description 51
- 210000001035 gastrointestinal tract Anatomy 0.000 claims abstract description 20
- 239000000463 material Substances 0.000 claims abstract description 15
- 230000007613 environmental effect Effects 0.000 claims abstract description 13
- 239000000843 powder Substances 0.000 claims description 92
- 238000002360 preparation method Methods 0.000 claims description 74
- 239000002994 raw material Substances 0.000 claims description 36
- 239000000341 volatile oil Substances 0.000 claims description 29
- 238000000034 method Methods 0.000 claims description 27
- 238000002156 mixing Methods 0.000 claims description 27
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 claims description 21
- 229920001202 Inulin Polymers 0.000 claims description 20
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 claims description 20
- 229940029339 inulin Drugs 0.000 claims description 20
- 244000179886 Moringa oleifera Species 0.000 claims description 19
- 235000011347 Moringa oleifera Nutrition 0.000 claims description 19
- 241000239366 Euphausiacea Species 0.000 claims description 18
- 240000008892 Helianthus tuberosus Species 0.000 claims description 18
- 235000003230 Helianthus tuberosus Nutrition 0.000 claims description 18
- 240000007849 Macleaya cordata Species 0.000 claims description 18
- 150000003904 phospholipids Chemical class 0.000 claims description 18
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 claims description 17
- 244000005700 microbiome Species 0.000 claims description 15
- 241000194032 Enterococcus faecalis Species 0.000 claims description 14
- 229940032049 enterococcus faecalis Drugs 0.000 claims description 14
- 240000006439 Aspergillus oryzae Species 0.000 claims description 13
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims description 13
- 244000063299 Bacillus subtilis Species 0.000 claims description 13
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 13
- 241000196324 Embryophyta Species 0.000 claims description 13
- 244000046146 Pueraria lobata Species 0.000 claims description 13
- 235000010575 Pueraria lobata Nutrition 0.000 claims description 13
- 150000001413 amino acids Chemical class 0.000 claims description 13
- 241000239370 Euphausia superba Species 0.000 claims description 11
- 235000010469 Glycine max Nutrition 0.000 claims description 11
- 244000068988 Glycine max Species 0.000 claims description 10
- GHVNFZFCNZKVNT-UHFFFAOYSA-N decanoic acid Chemical compound CCCCCCCCCC(O)=O GHVNFZFCNZKVNT-UHFFFAOYSA-N 0.000 claims description 10
- POULHZVOKOAJMA-UHFFFAOYSA-N dodecanoic acid Chemical compound CCCCCCCCCCCC(O)=O POULHZVOKOAJMA-UHFFFAOYSA-N 0.000 claims description 10
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- YWWVWXASSLXJHU-AATRIKPKSA-N (9E)-tetradecenoic acid Chemical compound CCCC\C=C\CCCCCCCC(O)=O YWWVWXASSLXJHU-AATRIKPKSA-N 0.000 claims description 8
- 240000008574 Capsicum frutescens Species 0.000 claims description 8
- 240000007232 Illicium verum Species 0.000 claims description 8
- 235000008227 Illicium verum Nutrition 0.000 claims description 8
- 238000010564 aerobic fermentation Methods 0.000 claims description 8
- 230000008901 benefit Effects 0.000 claims description 8
- 150000004667 medium chain fatty acids Chemical class 0.000 claims description 8
- 240000008042 Zea mays Species 0.000 claims description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 7
- 235000005822 corn Nutrition 0.000 claims description 7
- 230000036541 health Effects 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 6
- 239000005632 Capric acid (CAS 334-48-5) Substances 0.000 claims description 5
- 239000005635 Caprylic acid (CAS 124-07-2) Substances 0.000 claims description 5
- 235000002566 Capsicum Nutrition 0.000 claims description 5
- 239000005639 Lauric acid Substances 0.000 claims description 5
- 239000001390 capsicum minimum Substances 0.000 claims description 5
- 235000013312 flour Nutrition 0.000 claims description 5
- 229960002446 octanoic acid Drugs 0.000 claims description 5
- YWWVWXASSLXJHU-UHFFFAOYSA-N 9E-tetradecenoic acid Natural products CCCCC=CCCCCCCCC(O)=O YWWVWXASSLXJHU-UHFFFAOYSA-N 0.000 claims description 4
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims description 4
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims description 4
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims description 4
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims description 4
- 238000001179 sorption measurement Methods 0.000 claims description 4
- 239000004474 valine Substances 0.000 claims description 4
- 235000019742 Vitamins premix Nutrition 0.000 claims description 3
- 235000012054 meals Nutrition 0.000 claims description 3
- 239000011573 trace mineral Substances 0.000 claims description 3
- 235000013619 trace mineral Nutrition 0.000 claims description 3
- 230000000968 intestinal effect Effects 0.000 abstract description 26
- 230000001105 regulatory effect Effects 0.000 abstract description 11
- 230000001488 breeding effect Effects 0.000 abstract description 10
- 235000016709 nutrition Nutrition 0.000 abstract description 8
- 230000035764 nutrition Effects 0.000 abstract description 8
- 238000007726 management method Methods 0.000 abstract description 7
- 230000004060 metabolic process Effects 0.000 abstract description 7
- 230000003110 anti-inflammatory effect Effects 0.000 abstract description 6
- 244000052616 bacterial pathogen Species 0.000 abstract description 5
- 230000001276 controlling effect Effects 0.000 abstract description 5
- 235000004626 essential fatty acids Nutrition 0.000 abstract description 5
- 230000001737 promoting effect Effects 0.000 abstract description 5
- 230000003078 antioxidant effect Effects 0.000 abstract description 3
- 239000000047 product Substances 0.000 description 13
- 239000000203 mixture Substances 0.000 description 12
- 230000000052 comparative effect Effects 0.000 description 11
- 150000002148 esters Chemical class 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 241000282887 Suidae Species 0.000 description 7
- 230000009286 beneficial effect Effects 0.000 description 6
- 238000011161 development Methods 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 6
- 230000004888 barrier function Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 241000186660 Lactobacillus Species 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 4
- 210000004347 intestinal mucosa Anatomy 0.000 description 4
- 229940039696 lactobacillus Drugs 0.000 description 4
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 210000002249 digestive system Anatomy 0.000 description 3
- 239000003797 essential amino acid Substances 0.000 description 3
- 239000012467 final product Substances 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 230000035800 maturation Effects 0.000 description 3
- 230000003818 metabolic dysfunction Effects 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 230000036542 oxidative stress Effects 0.000 description 3
- 239000002504 physiological saline solution Substances 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 2
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 2
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 2
- 108090000695 Cytokines Proteins 0.000 description 2
- 102000004127 Cytokines Human genes 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 2
- 239000005642 Oleic acid Substances 0.000 description 2
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- 229930003451 Vitamin B1 Natural products 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 2
- 229960001231 choline Drugs 0.000 description 2
- 210000001072 colon Anatomy 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 229960000304 folic acid Drugs 0.000 description 2
- 235000019152 folic acid Nutrition 0.000 description 2
- 239000011724 folic acid Substances 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N glycerol group Chemical group OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 208000037817 intestinal injury Diseases 0.000 description 2
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 229960003512 nicotinic acid Drugs 0.000 description 2
- 235000001968 nicotinic acid Nutrition 0.000 description 2
- 239000011664 nicotinic acid Substances 0.000 description 2
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- 229960003495 thiamine Drugs 0.000 description 2
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 2
- 235000010374 vitamin B1 Nutrition 0.000 description 2
- 239000011691 vitamin B1 Substances 0.000 description 2
- TWJNQYPJQDRXPH-UHFFFAOYSA-N 2-cyanobenzohydrazide Chemical compound NNC(=O)C1=CC=CC=C1C#N TWJNQYPJQDRXPH-UHFFFAOYSA-N 0.000 description 1
- 108700016155 Acyl transferases Proteins 0.000 description 1
- 102000057234 Acyl transferases Human genes 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 238000008157 ELISA kit Methods 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 206010053759 Growth retardation Diseases 0.000 description 1
- 108010002352 Interleukin-1 Proteins 0.000 description 1
- 108010002350 Interleukin-2 Proteins 0.000 description 1
- 108090001005 Interleukin-6 Proteins 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 235000021360 Myristic acid Nutrition 0.000 description 1
- TUNFSRHWOTWDNC-UHFFFAOYSA-N Myristic acid Natural products CCCCCCCCCCCCCC(O)=O TUNFSRHWOTWDNC-UHFFFAOYSA-N 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 241000219780 Pueraria Species 0.000 description 1
- RXUWDKBZZLIASQ-UHFFFAOYSA-N Puerarin Natural products OCC1OC(Oc2c(O)cc(O)c3C(=O)C(=COc23)c4ccc(O)cc4)C(O)C(O)C1O RXUWDKBZZLIASQ-UHFFFAOYSA-N 0.000 description 1
- 108700005078 Synthetic Genes Proteins 0.000 description 1
- 229930003779 Vitamin B12 Natural products 0.000 description 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 229930003448 Vitamin K Natural products 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 210000001789 adipocyte Anatomy 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical group 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000002180 anti-stress Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- OGBUMNBNEWYMNJ-UHFFFAOYSA-N batilol Chemical class CCCCCCCCCCCCCCCCCCOCC(O)CO OGBUMNBNEWYMNJ-UHFFFAOYSA-N 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 1
- 230000000112 colonic effect Effects 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 230000023011 digestive tract development Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000006153 eosin methylene blue Substances 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 210000004211 gastric acid Anatomy 0.000 description 1
- 210000003736 gastrointestinal content Anatomy 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000034659 glycolysis Effects 0.000 description 1
- 231100000001 growth retardation Toxicity 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 210000005027 intestinal barrier Anatomy 0.000 description 1
- 230000007358 intestinal barrier function Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 229940106134 krill oil Drugs 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 230000037356 lipid metabolism Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000005228 liver tissue Anatomy 0.000 description 1
- 150000004668 long chain fatty acids Chemical class 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 229940057917 medium chain triglycerides Drugs 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 210000003240 portal vein Anatomy 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- HKEAFJYKMMKDOR-VPRICQMDSA-N puerarin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=CC(C2=O)=C1OC=C2C1=CC=C(O)C=C1 HKEAFJYKMMKDOR-VPRICQMDSA-N 0.000 description 1
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000011506 response to oxidative stress Effects 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 229940088197 riboflavin 3 mg Drugs 0.000 description 1
- 150000004671 saturated fatty acids Chemical class 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 150000004666 short chain fatty acids Chemical class 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 235000019163 vitamin B12 Nutrition 0.000 description 1
- 239000011715 vitamin B12 Substances 0.000 description 1
- 235000019158 vitamin B6 Nutrition 0.000 description 1
- 239000011726 vitamin B6 Substances 0.000 description 1
- QYSXJUFSXHHAJI-YRZJJWOYSA-N vitamin D3 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C\C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-YRZJJWOYSA-N 0.000 description 1
- 235000005282 vitamin D3 Nutrition 0.000 description 1
- 239000011647 vitamin D3 Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 235000019168 vitamin K Nutrition 0.000 description 1
- 239000011712 vitamin K Substances 0.000 description 1
- 150000003721 vitamin K derivatives Chemical class 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
- 229940021056 vitamin d3 Drugs 0.000 description 1
- 229940046010 vitamin k Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Birds (AREA)
- Mycology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Sustainable Development (AREA)
- Animal Behavior & Ethology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention relates to a breeding method for maintaining intestinal health of piglets and application thereof, wherein the breeding method for maintaining intestinal health of piglets comprises the following steps: special feed for feeding piglets: the special feed for the piglets comprises a base material, a fat compound, a fermentation compound and a premix; feeding mode: intermittent feeding limiting; and (3) environmental regulation: controlling temperature, humidity and CO in pig house 2 Concentration and NH 3 Concentration. The breeding method for promoting the intestinal health of the piglets organically combines the four modes of breeding, the ambient temperature, the air quality and the nutrition regulation and control, so that a more reasonable and scientific breeding management method is prepared, the metabolism of organisms can be effectively promoted, pathogenic bacteria can be effectively killed or prefabricated, free radicals in the bodies can be eliminated, the anti-inflammatory and antioxidant effects can be realized, and in addition, energy and essential fatty acid can be effectively provided for the intestinal tracts, intestinal flora can be regulated, and the intestinal health can be maintained.
Description
Technical Field
The invention belongs to the technical field of pig breeding, relates to a breeding method for maintaining intestinal health of piglets and application thereof, and in particular relates to a breeding method for regulating intestinal flora, maintaining intestinal health and improving piglet breeding benefits and application thereof.
Background
In order to improve the breeding benefit, the genetic improvement of pigs is always focused on improving the growth speed of the pigs, and a certain improvement effect is achieved. However, since the development of the digestive system of the piglets is not perfect, the intestinal flora is not fully established, and nutrition is often not absorbed effectively, so long-term high-load nutrition intake often causes intestinal damage of the piglets, and the health of the piglets is affected. Meanwhile, external factors such as weaning, diseases, high temperature, feed mildew and the like can cause a large number of free radicals to be generated in piglets, and oxidative stress reaction is induced. Oxidative stress breaks the redox balance state of animal intestinal tracts, and influences the content and activity of intestinal mucosa secretion oxidase. The weaned pig has incomplete intestinal development and weak self-body anti-stress capability, and when the weaned pig is subjected to oxidative stress, the barrier function of the intestinal tract is extremely easily damaged, so that the secretion of intestinal digestive enzymes is insufficient, and the digestion and absorption of nutrient substances are influenced.
The intestinal tract is a main place for digestion and absorption of nutrient substances, is also a natural physiological barrier for preventing external harmful substances from entering the animal body, and is an important immune organ of the body defense system. In the young growth stage, the intestinal tracts of piglets are extremely vulnerable to invasion of harmful substances, pathogenic bacteria and the like, oxidative stress injury is caused, intestinal barrier dysfunction is caused, and the intestinal tracts show inappetence and slow growth.
Many factors influence the intestinal health of piglets, including genetics, nutrition, feeding, etc. Nutrition, among them, is the most studied at present as an important influencing factor. For example, CN102870993A discloses a compound feed additive for effectively improving intestinal health of pigs, and the feed can achieve good diarrhea resistance through scientific compounding, effectively improve immunity of piglets, reduce use of antibiotic products, improve growth quality of the piglets, and lay a foundation for realization of production potential of the piglets; meanwhile, the phenomenon of growth retardation of piglets in the nursery stage and after the nursery stage is avoided; the environmental pollution is reduced.
The raising management of the piglets often affects the health and growth of the pigs, so the production management of the piglets is particularly important, however, in the research of the piglets, the raising management of the piglets is not paid attention to enough, more or the improvement of the growth of the piglets aiming at nutrition is realized, and the raising management and the raising technology are omitted, so that the raising benefit of the piglets often cannot reach expectations.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a breeding method for maintaining the intestinal health of piglets and application thereof, in particular to a breeding method for regulating intestinal flora, maintaining the intestinal health and improving the breeding benefit of piglets and application thereof.
In order to achieve the aim of the invention, the invention adopts the following technical scheme:
in a first aspect, the invention provides a method for maintaining intestinal health of piglets, which comprises the following steps:
special feed for feeding piglets: the special feed for the piglets comprises a base material, a fat compound, a fermentation compound and a premix;
feeding mode: intermittent feeding limiting;
and (3) environmental regulation: controlling temperature, humidity and CO in pig house 2 Concentration and NH 3 Concentration.
The breeding method for promoting the intestinal health of the piglets organically combines the four modes of breeding, the ambient temperature, the air quality and the nutrition regulation and control, so that a more reasonable and scientific breeding management method is prepared, the metabolism of organisms can be effectively promoted, pathogenic bacteria can be effectively killed or prefabricated, free radicals in the bodies can be eliminated, the anti-inflammatory and antioxidant effects can be realized, and in addition, energy and essential fatty acid can be effectively provided for the intestinal tracts, intestinal flora can be regulated, and the intestinal health can be maintained.
In order to pursue the maximum growth speed, piglets in a farm are mainly fed freely at present, but long-term free feeding can enable intestinal tracts of the piglets to be in a high-load state for a long time, so that the absorption of the intestinal tracts is affected.
The invention can provide optimum environment for piglets by regulating and controlling the environmental temperature, humidity and air quality of the pigsty, reduce the influence of environmental pathogenic factors on the immune system of the piglets and promote the development and maturation of the digestive system of the piglets.
The formula of the feed is characterized in that a fat compound and a fermentation compound are further added into a base material, wherein the fat compound is used as an energy supply substance to supply energy and essential fatty acid for intestinal tracts, so that the energy can be quickly supplied for the intestinal tracts of piglets, the recovery and growth of intestinal villi are promoted, and the health of the intestinal tracts is maintained; the fermentation compound is rich in various bioactive substances and high-quality fibers through fermentation, so that the intestinal microecological environment of an organism can be obviously improved, the growth of beneficial bacteria can be effectively increased, the field planting of harmful bacteria can be reduced, the intestinal mucosa barrier can be protected, and the problems of intestinal metabolic dysfunction, intestinal injury and the like can be avoided.
Preferably, the intermittent feeding limiting mode is as follows: free feeding is performed for 3-7 days (e.g. 3 days, 4 days, 5 days, 6 days, 7 days), and 1 day of feeding is limited to 70-80% (e.g. 70%, 72%, 75%, 78%, 80% etc.) of free feeding.
The intermittent feeding limiting mode is selected, so that the metabolism of organisms can be regulated more effectively, the intestinal burden of piglets is relieved, and the intestinal health is maintained.
Preferably, the feeding limiting mode is timing feeding, and the feeding is performed once in the morning, in the middle and in the evening, for example, the feeding is performed once in the range of about 8:00, about 14:00 and about 20:00.
Other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
Preferably, the temperature in the pig house is controlled at 22-26 ℃, such as 22 ℃, 23 ℃, 24 ℃,25 ℃, 26 ℃, etc.; humidity is controlled at 60-70%, e.g., 60%, 62%, 64%, 65%, 67%, 68%, 70%, etc.
Preferably, the CO in the pig house 2 The concentration is controlled at 1500-2000mg/m 3 For example 1500mg/m 3 、1600mg/m 3 、1700mg/m 3 、1800mg/m 3 、1900mg/m 3 、2000mg/m 3 Etc.; NH (NH) 3 The concentration is controlled to be 0.2-0.6mg/m 3 For example 0.2mg/m 3 、0.3mg/m 3 、0.4mg/m 3 、0.5mg/m 3 、0.6mg/m 3 Etc.
Other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
Selecting the above specific temperature, humidity and CO of pig house 2 Concentration, NH 3 The regulating and controlling numerical range of the concentration can better promote the elimination of pigletsThe development and maturation of the system are promoted, and the balance of intestinal flora and the intestinal health of piglets are further promoted.
Preferably, the fat complex comprises antarctic krill phospholipids, OMO structural lipids, inulin butyrate, coated plant essential oils, amino acids and a carrier.
Wherein the sn-1 position of the glycerin skeleton of the antarctic krill phospholipid is connected with saturated fatty acid, the sn-2 position is connected with unsaturated fatty acid, the oxidation is stable, and the PUFAs rich in n-3PUFAs, EPA, DHA, DPA and the like are concentrated at the sn-2 position of the glycerin skeleton and are the fatty acid essential for maintaining the internal balance of piglets. In addition, the antarctic krill phospholipid can also improve the activity of the intrahepatic lipid acyltransferase, regulate lipid metabolism and synthetic genes, has anti-inflammatory effect, and maintains the intestinal health of piglets.
The OMO structural ester is 1, 3-dioleoyl-2-medium chain fatty acid (OMO) structural ester, is symmetric medium-long chain triglyceride containing oleic acid at sn-1,3 positions and medium chain fatty acid at sn-2 positions, and is novel structural ester similar to breast milk structural ester OPO. This unique fatty acid profile aids in the absorption of nutrients and medium chain fatty acids are more readily digested and metabolized in the body than long chain fatty acids, especially in the form of sn-2 monoglycerides, which can be directly absorbed without re-esterification to triglycerides. The method has the advantages that oleic acid can be effectively absorbed after being hydrolyzed at sn-1 and 3 positions, and medium chain fatty acid can rapidly provide energy at sn-2 positions. The metabolic pathways of medium chain triglyceride and long chain triglyceride in vivo are different, and the long chain triglyceride is transported to mucous membrane cells through lymph after being decomposed to form new triglyceride; whereas medium chain triglycerides are hydrolysed by lipases to medium chain fatty acids which enter the liver from the portal vein where they are oxidized to H 2 O、CO 2 And energy or carcass formation, not readily stored in adipocytes or liver tissue. The OMO structural ester combines the advantages of the OMO structural ester and the OMO structural ester in vivo, overcomes the defects of the OMO structural ester, and the medium-chain fatty acid combined on the sn-2 position of the OMO structural ester can rapidly provide energy to avoid the reformation of triglyceride.
Wherein the inulin butyrate can improve the defect of directly supplementing short chain fatty acid (butyric acid), deliver butyric acid to intestinal tracts and promote the growth and development of intestinal villi of piglets. Inulin is not easy to digest in the oral cavity, the stomach and the small intestine, and can only reach the colon to generate glycolysis under the action of a large amount of beneficial bacteria to generate SCFA, so that supplementing inulin can obviously promote proliferation of intestinal probiotics such as bifidobacteria, lactobacillus and the like, and the SCFA generated by fermentation under the action of intestinal microorganisms can improve intestinal flora, promote growth and development of intestinal villi of piglets, absorb minerals and the like.
Wherein the plant essential oil and amino acids can assist in promoting fat metabolism, and further promote intestinal health.
Preferably, the fat compound comprises, in parts by weight, 10-20 parts of antarctic krill phospholipid, 20-40 parts of OMO structural fat, 5-10 parts of inulin butyrate, 0.1-1 part of coated plant essential oil, 5-15 parts of amino acid and 30-60 parts of carrier based on the total weight of the fat compound.
The weight parts of the euphausia superba phospholipids can be 10 parts, 12 parts, 15 parts, 18 parts, 20 parts and the like.
The weight parts of the inulin butyrate can be 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts and the like.
The OMO structural fat can be selected from 20 parts by weight, 23 parts by weight, 25 parts by weight, 30 parts by weight, 32 parts by weight, 35 parts by weight, 38 parts by weight, 40 parts by weight and the like.
The weight parts of the coated plant essential oil can be selected from 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 1 part and the like.
The weight parts of the amino acid can be 5 parts, 6 parts, 7 parts, 8 parts, 10 parts, 12 parts, 13 parts, 14 parts, 15 parts and the like.
The weight parts of the carrier can be 30 parts, 35 parts, 38 parts, 40 parts, 42 parts, 45 parts, 48 parts, 50 parts, 52 parts, 55 parts, 58 parts, 60 parts and the like.
Other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
Preferably, the medium chain fatty acid in the OMO structural ester comprises caprylic acid, capric acid, lauric acid and myristoleic acid, and the mass ratio of the caprylic acid, the capric acid, the lauric acid and the myristoleic acid is (1-3): 1-5): 1-2: 3-5.
Preferably, the coated plant essential oil comprises star anise essential oil and capsicum essential oil.
Preferably, the amino acids include leucine and valine.
Preferably, the carrier comprises puffed corn flour and/or puffed corncob meal.
Preferably, the fat complex is prepared by a preparation method comprising the steps of:
heating and dissolving antarctic krill phospholipid and OMO structural fat, uniformly mixing with inulin butyrate and amino acid, mixing with a carrier for adsorption, cooling, and uniformly mixing with coated plant essential oil.
Preferably, the heating means heating to 60-100deg.C, such as 60 ℃, 65 ℃, 70 ℃, 75 ℃, 80 ℃, 85 ℃, 90 ℃, 95 ℃, 100 ℃, etc.
Preferably, the cooling means cooling to 15-40 ℃, for example 15 ℃, 20 ℃,25 ℃, 30 ℃, 35 ℃, 40 ℃, etc.
Other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
Preferably, the fermentation complex is a product obtained from microbial fermentation of a fermentation feedstock;
the fermentation raw materials comprise at least two of soybean powder, euphausia superba powder, moringa oleifera leaf powder, kudzuvine root powder, jerusalem artichoke powder or macleaya cordata leaf powder.
Preferably, the fermentation raw material comprises a combination of soybean powder, euphausia superba powder, moringa oleifera leaf powder, kudzuvine root powder, jerusalem artichoke powder and macleaya cordata leaf powder.
Wherein the moringa She Fenfu contains moringa oleifera and has antibacterial and antiinflammatory effects; the pueraria powder is rich in puerarin, and can directly remove free radicals in a machine body, and has the effects of resisting inflammation and oxidation; the jerusalem artichoke powder is rich in inulin, water-soluble dietary fibers can not be hydrolyzed and digested by gastric acid, and can be utilized by beneficial microorganisms only in colon, so that the intestinal environment is improved; the main active ingredient of the macleaya cordata leaf powder is alkaloid, and has antibacterial and anti-inflammatory effects.
Preferably, the fermentation raw materials comprise the following components in parts by weight based on the total weight of the fermentation raw materials: 10-30 parts of soybean powder, 1-10 parts of euphausia superba powder, 30-50 parts of moringa oleifera leaf powder, 20-40 parts of radix puerariae powder, 5-10 parts of jerusalem artichoke powder and 10-30 parts of macleaya cordata leaf powder.
When the fermentation raw materials are combined in the specific mode, beneficial bacteria growth is increased, field planting of harmful bacteria is reduced, intestinal mucosa barriers are protected, and effects of avoiding intestinal metabolic dysfunction and intestinal damage are more remarkable.
The weight parts of the soybean powder can be 10 parts, 12 parts, 15 parts, 18 parts, 20 parts, 22 parts, 25 parts, 28 parts, 30 parts and the like.
The weight parts of the antarctic krill powder can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 7 parts, 8 parts, 9 parts, 10 parts and the like.
The weight parts of the moringa oleifera leaf powder can be 30 parts, 32 parts, 35 parts, 38 parts, 40 parts, 42 parts, 45 parts, 48 parts, 50 parts and the like.
The weight parts of the kudzuvine root powder can be 20 parts, 22 parts, 25 parts, 28 parts, 30 parts, 32 parts, 35 parts, 38 parts, 40 parts and the like.
The jerusalem artichoke powder can be selected from 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts and the like by weight.
The weight parts of the macleaya cordata leaf powder can be 10 parts, 12 parts, 15 parts, 18 parts, 20 parts, 22 parts, 25 parts, 28 parts, 30 parts and the like.
Other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
Preferably, the microorganism comprises a combination of enterococcus faecalis, bacillus subtilis and aspergillus oryzae.
Preferably, the microorganism comprises, in parts by weight, 10-30 parts of enterococcus faecalis, 20-40 parts of bacillus subtilis and 20-40 parts of aspergillus oryzae, based on the total weight of the microorganism.
The invention creatively carries out the combined fermentation treatment of bacteria and moulds, and selects the specific three strains, thereby having more excellent effect on the aspect of regulating intestinal health.
The enterococcus faecalis can be selected from 10 parts, 12 parts, 15 parts, 18 parts, 20 parts, 22 parts, 25 parts, 28 parts, 30 parts and the like.
The weight parts of the bacillus subtilis can be 20 parts, 22 parts, 25 parts, 28 parts, 30 parts, 32 parts, 35 parts, 38 parts, 40 parts and the like.
The weight parts of the aspergillus oryzae can be 20 parts, 22 parts, 25 parts, 28 parts, 30 parts, 32 parts, 35 parts, 38 parts, 40 parts and the like.
Preferably, the total weight of the microorganisms is 5-10%, such as 5%, 6%, 7%, 8%, 9%, 10%, etc., of the total weight of the fermentation feedstock.
Other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
Preferably, the fermentation complex is prepared by the following method:
mixing the fermentation raw materials with bacillus subtilis and aspergillus oryzae for aerobic fermentation, then mixing with enterococcus faecalis for anaerobic fermentation, and drying to obtain the finished product.
Preferably, the conditions of the aerobic fermentation are: the fermentation temperature is 25-40deg.C (such as 25deg.C, 30deg.C, 32deg.C, 33deg.C, 35deg.C, 37deg.C, 38deg.C, 40deg.C, etc.), the water content is 30-45% (such as 30%, 32%, 35%, 38%, 40%, 43%, 45%, etc.), and the fermentation time is 24-72h (such as 24h, 48h, 52h, 55h, 60h, 70h, 72h, etc.).
Preferably, the anaerobic fermentation conditions are: the fermentation temperature is 25-40deg.C (such as 25deg.C, 30deg.C, 32deg.C, 33deg.C, 35deg.C, 37deg.C, 38deg.C, 40deg.C, etc.), the water content is 30-45% (such as 30%, 32%, 35%, 38%, 40%, 43%, 45%, etc.), and the fermentation time is 48-96h (such as 48h, 52h, 55h, 60h, 70h, 80h, 90h, 96h, etc.).
The fermentation compound is prepared by the specific fermentation process, and has more remarkable effect in promoting the intestinal health of piglets.
Preferably, the base comprises corn.
Preferably, the premix comprises a vitamin premix and a trace element premix.
Preferably, the vitamin premix comprises any one or a combination of at least two of vitamin a, vitamin D3, vitamin E, vitamin K, vitamin B1, vitamin B6, vitamin B12, biotin, choline, riboflavin, folic acid, or niacin.
Preferably, the trace element premix comprises any one or a combination of at least two of I, fe, mn, se or Zn.
Preferably, the preparation method of the piglet special feed comprises the following steps:
and uniformly mixing the base material, the fat compound, the fermentation compound and the premix.
In a second aspect, the invention provides the use of the method for maintaining intestinal health of piglets according to the first aspect for improving the piglet cultivation benefits.
In a third aspect, the invention provides a feed for maintaining the intestinal health of piglets, which comprises a base material, a fat compound, a fermentation compound and a premix.
Preferably, the fat complex comprises antarctic krill phospholipids, OMO structural lipids, inulin butyrate, coated plant essential oils, amino acids and a carrier.
Preferably, the fat compound comprises, in parts by weight, 10-20 parts of antarctic krill phospholipid, 20-40 parts of OMO structural fat, 5-10 parts of inulin butyrate, 0.1-1 part of coated plant essential oil, 5-15 parts of amino acid and 30-60 parts of carrier based on the total weight of the fat compound.
Preferably, the coated plant essential oil comprises star anise essential oil and capsicum essential oil.
Preferably, the amino acids include leucine and valine.
Preferably, the carrier comprises puffed corn flour and/or puffed corncob meal.
Preferably, the fat complex is prepared by a preparation method comprising the steps of:
heating and dissolving antarctic krill phospholipid and OMO structural fat, uniformly mixing with inulin butyrate and amino acid, mixing with a carrier for adsorption, cooling, and uniformly mixing with coated plant essential oil.
Preferably, the fermentation complex is a product obtained from microbial fermentation of a fermentation feedstock;
the fermentation raw materials comprise at least two of soybean powder, euphausia superba powder, moringa oleifera leaf powder, kudzuvine root powder, jerusalem artichoke powder or macleaya cordata leaf powder.
Preferably, the fermentation raw material comprises a combination of soybean powder, euphausia superba powder, moringa oleifera leaf powder, kudzuvine root powder, jerusalem artichoke powder and macleaya cordata leaf powder.
Preferably, the fermentation raw materials comprise the following components in parts by weight based on the total weight of the fermentation raw materials: 10-30 parts of soybean powder, 1-10 parts of euphausia superba powder, 30-50 parts of moringa oleifera leaf powder, 20-40 parts of radix puerariae powder, 5-10 parts of jerusalem artichoke powder and 10-30 parts of macleaya cordata leaf powder.
Preferably, the microorganism comprises a combination of enterococcus faecalis, bacillus subtilis and aspergillus oryzae.
Preferably, the microorganism comprises, in parts by weight, 10-30 parts of enterococcus faecalis, 20-40 parts of bacillus subtilis and 20-40 parts of aspergillus oryzae, based on the total weight of the microorganism.
Preferably, the total weight of the microorganisms is 5-10% of the total weight of the fermentation feedstock.
Preferably, the fermentation complex is prepared by the following method:
mixing the fermentation raw materials with bacillus subtilis and aspergillus oryzae for aerobic fermentation, then mixing with enterococcus faecalis for anaerobic fermentation, and drying to obtain the finished product.
Compared with the prior art, the invention has the following beneficial effects:
the breeding method for promoting the intestinal health of the piglets organically combines the four modes of breeding, the ambient temperature, the air quality and the nutrition regulation and control, so that a more reasonable and scientific breeding management method is prepared, the metabolism of organisms can be effectively promoted, pathogenic bacteria can be effectively killed or prefabricated, free radicals in the bodies can be eliminated, the anti-inflammatory and antioxidant effects can be realized, and in addition, energy and essential fatty acid can be effectively provided for the intestinal tracts, intestinal flora can be regulated, and the intestinal health can be maintained.
The intermittent feeding-limiting mode adopted by the invention can regulate organism metabolism, relieve the intestinal burden of piglets and maintain intestinal health. The invention can provide optimum environment for piglets by regulating and controlling the environmental temperature, humidity and air quality of the pigsty, reduce the influence of environmental pathogenic factors on the immune system of the piglets and promote the development and maturation of the digestive system of the piglets. The formula of the feed is characterized in that a fat compound and a fermentation compound are further added into a base material, wherein the fat compound is used as an energy supply substance to supply energy and essential fatty acid for intestinal tracts, so that the energy can be quickly supplied for the intestinal tracts of piglets, the recovery and growth of intestinal villi are promoted, and the health of the intestinal tracts is maintained; the fermentation compound is rich in various bioactive substances and high-quality fibers through fermentation, so that the intestinal microecological environment of an organism can be obviously improved, the growth of beneficial bacteria can be effectively increased, the field planting of harmful bacteria can be reduced, the intestinal mucosa barrier can be protected, and the problems of intestinal metabolic dysfunction, intestinal injury and the like can be avoided.
Detailed Description
In order to further describe the technical means adopted by the present invention and the effects thereof, the following describes the technical scheme of the present invention in combination with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.
The starting materials for the preparation examples and comparative examples described below are all commercially available or are obtained by conventional techniques known to those skilled in the art in combination with the disclosures of the prior art.
The viable count of the enterococcus faecalis raw material in the following preparation example is 10 11 The viable count in CFU/g and bacillus subtilis raw material is 10 11 CFU/g, number of spores in Aspergillus oryzae raw material is 2×10 10 CFU/g。
Euphausia superba phospholipids are products available from the company Sitavid biotechnology, inc.
Inulin butyrate is a product purchased from Hebei's source biotechnology Co.
The star anise essential oil is a product purchased from the company ediblet biotechnology limited in peninsula.
The capsicum essential oil is a product purchased from ediblet biotechnology limited in peninsula.
Moringa oleifera leaf powder is a product purchased from western security australian technologies.
The radix Puerariae powder is purchased from Fu Yi bang biotechnology Co., ltd.
Jerusalem artichoke powder is a product purchased from the company of the biological engineering of Siam Rayleigh.
Macleaya cordata leaf powder is a product purchased from the biological sciences company of rest of Fennernst.
Preparation example 1
The preparation example provides a piglet special feed which is prepared by uniformly mixing 50 parts of corn, 5 parts of fat compound, 40 parts of fermentation compound and 5 parts of premix in parts by weight;
based on the total weight of the fat compound, the preparation method of the fat compound comprises the following steps: mixing 10 parts of antarctic krill phospholipid, 30 parts of OMO structural fat and 10 parts of inulin butyrate, heating to 70 ℃ to dissolve completely, mixing with 40 parts of puffed corn flour for adsorption, cooling to 25 ℃, and then uniformly mixing with 5 parts of leucine, 5 parts of valine, 0.5 part of star anise essential oil and 0.5 part of capsicum essential oil to obtain the antarctic krill oil; wherein the medium chain fatty acid raw materials in the OMO structural fat are caprylic acid, capric acid, lauric acid and myristic acid with the mass ratio of 2:4:1:4;
based on the total weight of the fermentation compound, the preparation method of the fermentation compound comprises the following steps: mixing 20 parts of soybean powder, 10 parts of antarctic krill powder, 40 parts of moringa oleifera leaf powder, 30 parts of kudzuvine root powder, 10 parts of jerusalem artichoke powder and 20 parts of macleaya cordata leaf powder with 30 parts of bacillus subtilis and 30 parts of aspergillus oryzae, performing aerobic fermentation at 35 ℃ for 48 hours with water content of 35%, then mixing with 20 parts of enterococcus faecalis, performing anaerobic fermentation at 35 ℃ with water content of 40%, and performing fermentation for 72 hours; drying to obtain the final product;
the premix comprises VA 15000IU/kg, VD3 3000IU/kg, VE 100mg/kg, VK 2mg/kg, vitamin B1 3mg/kg, VB6 1mg/kg, VB12 μg/kg, biotin 0.5mg/kg, choline 0.4g/kg, riboflavin 3mg/kg, folic acid 3mg/kg, nicotinic acid 20mg/kg, I0.3 mg/kg, fe 80mg/kg, mn 30mg/kg, se 0.5mg/kg and Zn 60mg/kg.
Preparation example 2
The preparation example provides a piglet special feed, the composition formula of which is different from that of preparation example 1 only in that the fermentation raw materials of the fermentation compound are different, and the feed consists of 50 parts of kudzuvine root powder, 17 parts of jerusalem artichoke powder and 33 parts of macleaya cordata leaf powder. Other materials and preparation processes were as in example 1.
Preparation example 3
The preparation example provides a piglet special feed, the composition formula of which is different from that of preparation example 1 only in that the fermentation raw materials of the fermentation compound are different, and the feed consists of 57 parts of moringa oleifera leaf powder, 14 parts of jerusalem artichoke powder and 29 parts of macleaya cordata leaf powder. Other materials and preparation processes were as in example 1.
Preparation example 4
The preparation example provides a piglet special feed, the composition formula of which is different from that of preparation example 1 only in that the fermentation raw materials of the fermentation compound are different, and the piglet special feed consists of 44 parts of moringa oleifera leaf powder, 33 parts of kudzuvine root powder and 23 parts of macleaya cordata leaf powder. Other materials and preparation processes were as in example 1.
Preparation example 5
The preparation example provides a piglet special feed, the composition formula of which is different from that of preparation example 1 only in that the fermentation raw materials of the fermentation compound are different, and the piglet special feed consists of 50 parts of moringa oleifera leaf powder, 37.5 parts of kudzuvine root powder and 12.5 parts of jerusalem artichoke powder. Other materials and preparation processes were as in example 1.
Preparation example 6
The preparation example provides a piglet special feed, the composition formula of which is different from that of preparation example 1 only in the fermentation process of the fermentation compound, and the preparation method of the fermentation compound comprises the following steps: mixing 40 parts of moringa oleifera leaf powder, 30 parts of kudzuvine root powder, 10 parts of jerusalem artichoke powder, 20 parts of macleaya cordata leaf powder and 60 parts of bacillus subtilis, performing aerobic fermentation at 35 ℃ for 48 hours with the water content of 35%, mixing with 20 parts of enterococcus faecalis, and performing anaerobic fermentation at 35 ℃ with the water content of 40% for 72 hours; drying to obtain the final product. Other materials and preparation processes were as in example 1.
Preparation example 7
The preparation example provides a piglet special feed, the composition formula of which is different from that of preparation example 1 only in the fermentation process of the fermentation compound, and the preparation method of the fermentation compound comprises the following steps: mixing 40 parts of moringa oleifera leaf powder, 30 parts of kudzuvine root powder, 10 parts of jerusalem artichoke powder, 20 parts of macleaya cordata leaf powder and 60 parts of aspergillus oryzae, performing aerobic fermentation at 35 ℃ for 48 hours with the water content of 35%, then mixing with 20 parts of enterococcus faecalis, performing anaerobic fermentation at 35 ℃ with the water content of 40%, and performing fermentation for 72 hours; drying to obtain the final product. Other materials and preparation processes were as in example 1.
Preparation example 8
The preparation example provides a piglet special feed, the composition formula of which is different from that of preparation example 1 only in that the preparation raw materials of the fat compound, namely 10 parts of antarctic krill phospholipid, 30 parts of OMO structural fat, 10 parts of inulin butyrate, 37.5 parts of OMO structural fat and 12.5 parts of inulin butyrate, are replaced by the raw materials of the fat compound, and other raw materials and the preparation process are the same as those of the preparation example 1.
Preparation example 9
The preparation example provides a piglet special feed, the composition formula of which is different from that of preparation example 1 only in that the preparation raw materials of the fat compound, namely 10 parts of antarctic krill phospholipids, 30 parts of OMO structural fat, 10 parts of inulin butyrate, 25 parts of antarctic krill phospholipids and 25 parts of inulin butyrate, are replaced, and other raw materials and the preparation process are identical to those of the preparation example 1.
Preparation example 10
The preparation example provides a piglet special feed, the composition formula of which is different from that of preparation example 1 only in that the preparation raw materials of the fat compound, namely 10 parts of antarctic krill phospholipid, 30 parts of OMO structural fat, 10 parts of inulin butyrate, 12.5 parts of antarctic krill phospholipid and 37.5 parts of OMO structural fat, are replaced, and other raw materials and preparation processes are identical to those of the preparation example 1.
PREPARATION EXAMPLE 11
The preparation example provides a piglet special feed, the composition formula of which is different from that of the preparation example 1 only in that the preparation raw materials of the fat compound, namely 0.5 part of star anise essential oil and 0.5 part of chilli essential oil, are replaced by 1 part of star anise essential oil, and other raw materials and the preparation process are identical with those of the preparation example 1.
Preparation example 12
The preparation example provides a piglet special feed, the composition formula of which is different from that of the preparation example 1 only in that the preparation raw materials of the fat compound, namely 0.5 part of star anise essential oil and 0.5 part of chilli essential oil, are replaced by 1 part of chilli essential oil, and other raw materials and the preparation process are identical to those of the preparation example 1.
Example 1
The embodiment provides a breeding method for maintaining intestinal health of piglets, which comprises the following steps:
feeding special fattening pig feed of preparation example 1;
the following feeding modes are adopted: feeding is limited at 1 day (feeding is performed 1 time in 8:00, 14:00 and 20:00) with feeding amount being 75% of free feeding amount.
The environmental control mode is as follows: the temperature in the pigsty is controlled at 23 ℃; the humidity is controlled at 65%; CO 2 The concentration is controlled at 1700mg/m 3 ；NH 3 The concentration is controlled at 0.4mg/m 3 。
Example 2
The embodiment provides a breeding method for maintaining intestinal health of piglets, which comprises the following steps:
feeding special fattening pig feed of preparation example 1;
the following feeding modes are adopted: feeding is limited in 1 day (feeding is performed 1 time in 8:00, 14:00 and 20:00) with feeding amount of 70% of free feeding amount.
The environmental control mode is as follows: the temperature in the pigsty is controlled at 24 ℃; the humidity is controlled at 70%; CO 2 The concentration is controlled at 1500mg/m 3 ；NH 3 The concentration is controlled at 0.3mg/m 3 。
Examples 3 to 13
This example provides a method of raising piglets for maintaining intestinal health, which differs from example 1 only in that the piglet-specific feed of preparation example 1 is replaced with the piglet-specific feeds of preparations 2 to 12, and the other operations remain unchanged.
Comparative example 1
The comparative example provides a piglet breeding method, which comprises the following steps:
feeding commercial fattening pig feed (Aoni biotechnology Co., guangzhou);
the following feeding modes are adopted: feeding is limited at 1 day (feeding is performed 1 time in 8:00, 14:00 and 20:00) with feeding amount being 75% of free feeding amount.
The environmental control mode is as follows: the temperature in the pigsty is controlled at 23 ℃; the humidity is controlled at 65%; CO 2 The concentration is controlled at 1700mg/m 3 ；NH 3 The concentration is controlled at 0.4mg/m 3 。
Comparative example 2
The comparative example provides a piglet breeding method, which comprises the following steps:
feeding special fattening pig feed of preparation example 1;
adopting a free feeding mode;
the environmental control mode is as follows: the temperature in the pigsty is controlled at 23 ℃; the humidity is controlled at 65%; CO 2 The concentration is controlled at 1700mg/m 3 ；NH 3 The concentration is controlled at 0.4mg/m 3 。
Comparative example 3
The comparative example provides a piglet breeding method, which comprises the following steps:
feeding special fattening pig feed of preparation example 1;
the following feeding modes are adopted: feeding is limited at 1 day (feeding is performed 1 time in 8:00, 14:00 and 20:00) with feeding amount being 75% of free feeding amount.
The environmental control mode is as follows: the temperature in the pig house is controlled at 28 ℃; the humidity is controlled at 55%; CO 2 The concentration is controlled at 1700mg/m 3 ；NH 3 The concentration is controlled at 0.4mg/m 3 。
Comparative example 4
The comparative example provides a piglet breeding method, which comprises the following steps:
feeding special fattening pig feed of preparation example 1;
the following feeding modes are adopted: feeding is limited at 1 day (feeding is performed 1 time in 8:00, 14:00 and 20:00) with feeding amount being 75% of free feeding amount.
The environment is regulated in the following way: the temperature in the pigsty is controlled at 23 ℃; the humidity is controlled at 65%; not to CO 2 Concentration and NH 3 The concentration was controlled.
Test case
170 long and large three-way pigs with healthy weaning time and weight of about 8kg are selected and randomly divided into 17 treatment groups, and the culture modes of examples 1-13 and comparative examples 1-4 are adopted for culture, and the test lasts for 28 days.
(1) The growth performance was counted and the statistical results are shown in table 1 (presented as average values).
TABLE 1
(2) At the end of the test, 3 piglets close to average weight were slaughtered, cecal and colonic contents were collected by aseptic technique, shaken well in sterilized physiological saline, and the numbers of Lactobacillus entericus and E.coli were counted by plate culture counting method, the number of flora was expressed as logarithm of the total number of bacterial communities contained in each gram of intestinal contents (lgCFU/g)]And (3) representing. 1g of the content is weighed in a sterile operation table and uniformly shaken in 99mL of sterilized physiological saline with glass beads, then 1mL of the content is taken in a test tube, 9mL of sterile 0.9% physiological saline is added, and the mixture is uniformly shaken and diluted to 10 according to 10 times of the equal ratio -7 . Wherein lactobacillus is inoculated on a lactobacillus selective medium (LBS) for culture, and colony counting is carried out after anaerobic culture for 48 hours in a biochemical incubator at 37 ℃; coli was inoculated on eosin-methylene blue agar medium and cultured, and colony counting was performed after aerobic culture in a biochemical incubator at 37℃for 24 hours. The statistical results are shown in table 2 (presented as average values).
TABLE 2
(3) After the test, blood is collected from the anterior vena cava of each group of pigs, the pigs are kept stand at 25 ℃, are centrifuged for 10min at 1500 Xg, and serum is collected and is frozen at-80 ℃ for preservation to be tested. Serum cytokines were assayed using commercial pig-specific ELISA kits and serum cytokine (IL-1. Beta., IL-2, IL-6 and TNF-. Alpha.) levels were determined strictly according to the kit instructions. The statistical results are shown in table 3 (presented as average values).
TABLE 3 Table 3
From the data in tables 1-3, it can be seen that: compared with the feeding mode of the comparative example, the feeding mode of the invention can more effectively promote organism metabolism, effectively kill or prefabricate pathogenic bacteria, remove free radicals in vivo, resist inflammation and oxidization, regulate intestinal flora and maintain intestinal health, thereby remarkably reducing the feed ratio and improving the growth performance of piglets. And the fermentation compound, the fat compound, the feeding mode and the environmental factors can influence the technical effects.
The applicant states that the present invention is described by way of the above examples as a method of raising a piglet to maintain intestinal health and its use, but the present invention is not limited to the above examples, i.e. it is not meant that the present invention must be carried out in dependence on the above examples. It should be apparent to those skilled in the art that any modification of the present invention, equivalent substitution of raw materials for the product of the present invention, addition of auxiliary components, selection of specific modes, etc., falls within the scope of the present invention and the scope of disclosure.
The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the specific details of the above embodiments, and various simple modifications can be made to the technical solution of the present invention within the scope of the technical concept of the present invention, and all the simple modifications belong to the protection scope of the present invention.
In addition, the specific features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various possible combinations are not described further.
Claims (18)
1. The breeding method for maintaining the intestinal health of the piglets is characterized by comprising the following steps of:
special feed for feeding piglets: the special feed for the piglets comprises a base material, a fat compound, a fermentation compound and a premix;
based on the total weight of the fat compound, the fat compound comprises 10-20 parts of antarctic krill phospholipid, 20-40 parts of OMO structural fat, 5-10 parts of inulin butyrate, 0.1-1 part of coated plant essential oil, 5-15 parts of amino acid and 30-60 parts of carrier in parts by weight;
the fermentation compound is a product obtained by fermenting a fermentation raw material by microorganisms;
the fermentation raw materials comprise at least two of soybean powder, euphausia superba powder, moringa oleifera leaf powder, kudzuvine root powder, jerusalem artichoke powder or macleaya cordata leaf powder; the microorganism comprises a combination of enterococcus faecalis, bacillus subtilis and aspergillus oryzae;
the premix comprises a vitamin premix and a trace element premix;
feeding mode: intermittent feeding limiting modes are as follows: feeding freely for 3-7 days, wherein the feeding limit is 70-80% of the feeding freely for 1 day, and the feeding is reciprocated;
and (3) environmental regulation: controlling temperature in pig house at 22-26deg.C, humidity at 60-70%, and CO 2 The concentration is 1500-2000mg/m 3 、NH 3 The concentration is 0.2-0.6mg/m 3 。
2. The method for raising a piglet's intestinal health according to claim 1, wherein the medium-chain fatty acid in the OMO structural fat comprises caprylic acid, capric acid, lauric acid and myristoleic acid, and the mass ratio of caprylic acid, capric acid, lauric acid and myristoleic acid is (1-3): 1-5): 1-2: 3-5.
3. The method of claim 1, wherein the coated plant essential oil comprises star anise essential oil and capsicum essential oil.
4. The method of claim 1, wherein the amino acids include leucine and valine.
5. A method of raising a piglet intestinal tract health according to claim 1 wherein the carrier comprises puffed corn flour and/or puffed corncob meal.
6. The method of claim 1, wherein the fat complex is prepared by a preparation method comprising the steps of:
heating and dissolving antarctic krill phospholipid and OMO structural fat, uniformly mixing with inulin butyrate and amino acid, mixing with a carrier for adsorption, cooling, and uniformly mixing with coated plant essential oil.
7. A method of raising a piglet intestinal tract health according to claim 6 wherein said heating is to a temperature of 60-100 ℃.
8. A method of raising a piglet intestinal tract health according to claim 6 wherein said cooling is to 15-40 ℃.
9. The method of claim 1, wherein the fermentation feedstock comprises a combination of soy flour, euphausia superba powder, moringa oleifera leaf powder, kudzuvine root powder, jerusalem artichoke powder, and macleaya cordata leaf powder.
10. The method for raising piglets in intestinal health according to claim 9, wherein the fermented raw materials comprise, in parts by weight, based on the total weight of the fermented raw materials: 10-30 parts of soybean powder, 1-10 parts of euphausia superba powder, 30-50 parts of moringa oleifera leaf powder, 20-40 parts of radix puerariae powder, 5-10 parts of jerusalem artichoke powder and 10-30 parts of macleaya cordata leaf powder.
11. The method for raising a piglet's intestinal health according to claim 1, wherein the microorganisms comprise enterococcus faecalis 10-30 parts, bacillus subtilis 20-40 parts and aspergillus oryzae 20-40 parts in parts by weight based on the total weight of the microorganisms.
12. The method of claim 1, wherein the total weight of the microorganisms is 5-10% of the total weight of the fermentation feedstock.
13. The method of claim 1, wherein the fermentation complex is prepared by the following method:
mixing the fermentation raw materials with bacillus subtilis and aspergillus oryzae for aerobic fermentation, then mixing with enterococcus faecalis for anaerobic fermentation, and drying to obtain the finished product.
14. The method for maintaining intestinal health of piglets according to claim 13, wherein the aerobic fermentation conditions are as follows: the fermentation temperature is 25-40 ℃, the water content is 30-45%, and the fermentation time is 24-72h.
15. The method of claim 13, wherein the anaerobic fermentation conditions are: the fermentation temperature is 25-40 ℃, the water content is 30-45%, and the fermentation time is 48-96h.
16. A method of raising a piglet's intestinal health according to claim 1, wherein said base material comprises corn.
17. The method for raising piglets for maintaining intestinal health as claimed in claim 1, wherein the preparation method of the piglet special feed comprises the following steps:
and uniformly mixing the base material, the fat compound, the fermentation compound and the premix.
18. Use of a method of raising piglets according to any one of claims 1-17 for maintaining intestinal health of piglets for improving the benefits of piglet raising.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210672530.5A CN115005159B (en) | 2022-06-14 | 2022-06-14 | Breeding method for maintaining intestinal health of piglets and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210672530.5A CN115005159B (en) | 2022-06-14 | 2022-06-14 | Breeding method for maintaining intestinal health of piglets and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115005159A CN115005159A (en) | 2022-09-06 |
| CN115005159B true CN115005159B (en) | 2023-08-15 |
Family
ID=83074037
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210672530.5A Active CN115005159B (en) | 2022-06-14 | 2022-06-14 | Breeding method for maintaining intestinal health of piglets and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115005159B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11289994A (en) * | 1998-04-09 | 1999-10-26 | Hideaki Tanaka | Formula feed containing additive having balanced nutrition |
| BR0104925A (en) * | 2001-10-26 | 2003-08-26 | Gilson Cesar Vituri | Animal food |
| CN106834163A (en) * | 2016-12-20 | 2017-06-13 | 湖南赛福资源饲料科技有限公司 | Feed fermentation agent, fermented feed and preparation method thereof |
| KR101895039B1 (en) * | 2018-06-14 | 2018-09-07 | 정창호 | Bad Smell Reducing Apparatus using Complex Lactic Acid Bacteria |
| CN113632888A (en) * | 2021-08-10 | 2021-11-12 | 广州朴成生物科技有限公司 | Functional additive for reducing weaning stress of piglets and preparation method and application thereof |
| CN113994923A (en) * | 2021-11-08 | 2022-02-01 | 广州朴成生物科技有限公司 | Breeding boar propagation promoting prevention and control method |
| CN113994924A (en) * | 2021-11-08 | 2022-02-01 | 广州朴成生物科技有限公司 | Backup sow breeding promotion prevention and control method |
-
2022
- 2022-06-14 CN CN202210672530.5A patent/CN115005159B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11289994A (en) * | 1998-04-09 | 1999-10-26 | Hideaki Tanaka | Formula feed containing additive having balanced nutrition |
| BR0104925A (en) * | 2001-10-26 | 2003-08-26 | Gilson Cesar Vituri | Animal food |
| CN106834163A (en) * | 2016-12-20 | 2017-06-13 | 湖南赛福资源饲料科技有限公司 | Feed fermentation agent, fermented feed and preparation method thereof |
| KR101895039B1 (en) * | 2018-06-14 | 2018-09-07 | 정창호 | Bad Smell Reducing Apparatus using Complex Lactic Acid Bacteria |
| CN113632888A (en) * | 2021-08-10 | 2021-11-12 | 广州朴成生物科技有限公司 | Functional additive for reducing weaning stress of piglets and preparation method and application thereof |
| CN113994923A (en) * | 2021-11-08 | 2022-02-01 | 广州朴成生物科技有限公司 | Breeding boar propagation promoting prevention and control method |
| CN113994924A (en) * | 2021-11-08 | 2022-02-01 | 广州朴成生物科技有限公司 | Backup sow breeding promotion prevention and control method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115005159A (en) | 2022-09-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108813289B (en) | Application method and preparation method of preparation for efficiently biodegrading mycotoxin | |
| CN102356816B (en) | Animal health care probiotics leavening capable of improving meat quality and application thereof | |
| CN106721260A (en) | A kind of preparation and its application of anti diar rhea type piglet specific complex probiotics | |
| CN110604226A (en) | Diarrhea-preventing growth-promoting composite feed additive for weaned piglets and preparation method thereof | |
| CN101683125A (en) | Method for preparing biological fermentation feed for pigs | |
| CN103535323A (en) | Livestock and poultry breeding mode | |
| CN103005159A (en) | Preparation method of ginkgo leaf biological feed additive | |
| CN108813123A (en) | It is a kind of improve intestine of young pigs health enteral nutrition packet and application | |
| CN102356829A (en) | Growing-finishing pig later-stage microorganism fermentation antibiotics-free feed | |
| CN102422996A (en) | Preliminary microbial fermentation antibiotic-free feed for growth fattening pigs | |
| CN103766604A (en) | Preparation of composite probiotic preparation | |
| CN105851485A (en) | Liquid complex probiotic preparation and preparation method thereof | |
| CN103232959A (en) | Preparation method and application of feeding bacillus licheniformis inoculant | |
| KR101427187B1 (en) | Composition for Eco-friendly Microorganism Formulation Comprising Microorganism Probiotics as Effective Ingredient and Production Method Thereof | |
| CN106578354A (en) | Piglet compound premix, preparation method and daily ration for piglet | |
| CN101874812B (en) | Composite bacteria preparation and application thereof in weight increment of immature livestock and control of diarrhea | |
| CN108902445A (en) | A kind of improvement intestine of young pigs health additive and preparation method thereof | |
| CN115005159B (en) | Breeding method for maintaining intestinal health of piglets and application thereof | |
| CN114304413B (en) | Composite containing acidifying agent and preparation method thereof | |
| CN105961865B (en) | Composite microecological preparation and application | |
| CN109105635A (en) | A kind of additive for farm animal feed with bacteriostasis containing allicin and capsaicine | |
| CN103710413A (en) | Preparation method of lactobacillus peptide as well as application of lactobacillus peptide in animal feed | |
| CN108815201B (en) | Biological veterinary drug for preventing and treating white scour of piglets and preparation method thereof | |
| CN112369528A (en) | Microbial preparation for improving intestinal environment of poultry and preparation method thereof | |
| CN111763638A (en) | Veterinary microecological preparation and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |