CN112369528A - Microbial preparation for improving intestinal environment of poultry and preparation method thereof - Google Patents
Microbial preparation for improving intestinal environment of poultry and preparation method thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 30
- 230000000813 microbial effect Effects 0.000 title claims abstract description 23
- 244000144977 poultry Species 0.000 title claims abstract description 22
- 230000000968 intestinal effect Effects 0.000 title claims abstract description 20
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 13
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 12
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 12
- 241000194032 Enterococcus faecalis Species 0.000 claims abstract description 12
- 229940032049 enterococcus faecalis Drugs 0.000 claims abstract description 12
- 241000193749 Bacillus coagulans Species 0.000 claims abstract description 11
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 11
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims abstract description 11
- 229940054340 bacillus coagulans Drugs 0.000 claims abstract description 11
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims abstract description 11
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 claims abstract description 10
- 241000081271 Phaffia rhodozyma Species 0.000 claims abstract description 10
- 235000013793 astaxanthin Nutrition 0.000 claims abstract description 10
- 239000001168 astaxanthin Substances 0.000 claims abstract description 10
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 claims abstract description 10
- 229940022405 astaxanthin Drugs 0.000 claims abstract description 10
- 238000004519 manufacturing process Methods 0.000 claims abstract description 4
- 239000002207 metabolite Substances 0.000 claims abstract description 3
- 238000000855 fermentation Methods 0.000 claims description 39
- 230000004151 fermentation Effects 0.000 claims description 39
- 239000001963 growth medium Substances 0.000 claims description 39
- 238000011218 seed culture Methods 0.000 claims description 34
- 241000894006 Bacteria Species 0.000 claims description 27
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 241000186660 Lactobacillus Species 0.000 claims description 18
- 229940039696 lactobacillus Drugs 0.000 claims description 18
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 16
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- 239000001888 Peptone Substances 0.000 claims description 12
- 108010080698 Peptones Proteins 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 12
- 235000019319 peptone Nutrition 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 11
- 230000001954 sterilising effect Effects 0.000 claims description 11
- 244000199885 Lactobacillus bulgaricus Species 0.000 claims description 10
- 239000002609 medium Substances 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 9
- 239000004310 lactic acid Substances 0.000 claims description 8
- 235000014655 lactic acid Nutrition 0.000 claims description 8
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 6
- 235000015278 beef Nutrition 0.000 claims description 6
- 229940041514 candida albicans extract Drugs 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 229940099596 manganese sulfate Drugs 0.000 claims description 6
- 239000011702 manganese sulphate Substances 0.000 claims description 6
- 235000007079 manganese sulphate Nutrition 0.000 claims description 6
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 239000012138 yeast extract Substances 0.000 claims description 6
- 235000000346 sugar Nutrition 0.000 claims description 4
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- 244000061456 Solanum tuberosum Species 0.000 claims description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 3
- 230000003213 activating effect Effects 0.000 claims description 3
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 3
- KLOIYEQEVSIOOO-UHFFFAOYSA-N carbocromen Chemical compound CC1=C(CCN(CC)CC)C(=O)OC2=CC(OCC(=O)OCC)=CC=C21 KLOIYEQEVSIOOO-UHFFFAOYSA-N 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 235000012015 potatoes Nutrition 0.000 claims description 3
- 239000001632 sodium acetate Substances 0.000 claims description 3
- 235000017281 sodium acetate Nutrition 0.000 claims description 3
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 2
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 2
- 238000004321 preservation Methods 0.000 claims description 2
- 230000001502 supplementing effect Effects 0.000 claims description 2
- 241000272496 Galliformes Species 0.000 claims 2
- 241000271566 Aves Species 0.000 claims 1
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- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 14
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- 241000186672 Lactobacillus delbrueckii subsp. bulgaricus Species 0.000 abstract 1
- 235000013594 poultry meat Nutrition 0.000 description 14
- 241000287828 Gallus gallus Species 0.000 description 7
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- 238000009395 breeding Methods 0.000 description 4
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- 244000005700 microbiome Species 0.000 description 4
- 241001148470 aerobic bacillus Species 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 235000013330 chicken meat Nutrition 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
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- 241000238557 Decapoda Species 0.000 description 1
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- 229930182558 Sterol Natural products 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000003975 animal breeding Methods 0.000 description 1
- 235000010208 anthocyanin Nutrition 0.000 description 1
- 239000004410 anthocyanin Substances 0.000 description 1
- 229930002877 anthocyanin Natural products 0.000 description 1
- 150000004636 anthocyanins Chemical class 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
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- PGBHMTALBVVCIT-VCIWKGPPSA-N framycetin Chemical compound N[C@@H]1[C@@H](O)[C@H](O)[C@H](CN)O[C@@H]1O[C@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](N)C[C@@H](N)[C@@H]2O)O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CN)O2)N)O[C@@H]1CO PGBHMTALBVVCIT-VCIWKGPPSA-N 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 208000028774 intestinal disease Diseases 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
Abstract
The invention discloses a microbial preparation for improving the intestinal environment of poultry, which is characterized by comprising the following components in part by weight: comprises candida utilis, bacillus subtilis, bacillus coagulans, bacillus licheniformis, lactobacillus bulgaricus, enterococcus faecalis and metabolites thereof and phaffia rhodozyma astaxanthin. The probiotics of the invention can improve the production performance of animals and disease resistance by improving the micro-ecological environment of animal intestinal tracts and improving the immunity of the animals, and has no residue in animal products and good economic and ecological benefits.
Description
Technical Field
The invention relates to a microbial preparation for improving the intestinal environment of poultry and a preparation method thereof, belonging to the field of animal breeding preparations.
Background
At present, poultry breeding is developed towards intensive breeding, because of high breeding density and high infectious disease incidence, people usually adopt antibiotics to inhibit or kill harmful microorganisms in the digestive tract so as to improve the production performance of poultry. But simultaneously, a large number of drug-resistant bacterial strains are formed, so that the control and treatment of animal diseases are more difficult, and the microecological balance of the gastrointestinal tract can be disturbed, so that the sensitivity of animals, particularly young animals, on pathogenic microorganisms is increased, and the occurrence of digestive tract diseases is increased.
The microbial preparation is a novel green preparation developed in recent years, is non-toxic and residue-free, can regulate animal intestinal flora, inhibit harmful bacteria, promote growth and development, improve the ecological environment of livestock and poultry breeding, achieve the aim of ecological prevention and control, and has good economic benefit and ecological benefit.
Disclosure of Invention
In view of the above technical problems, the present invention aims to provide a microbial preparation for improving the intestinal environment of poultry, and the present invention also provides a preparation method of the microbial preparation.
In order to achieve the purpose, the technical scheme of the invention is as follows: a microbial preparation for improving the intestinal environment of poultry is characterized in that: comprises candida utilis, bacillus subtilis, bacillus coagulans, bacillus licheniformis, lactobacillus bulgaricus, enterococcus faecalis and metabolites thereof and phaffia rhodozyma astaxanthin.
In the scheme, the method comprises the following steps: comprises 1-4mg/L astaxanthin produced by phaffia rhodozyma and 1x10 or more Candida utilis8cfu/ml, bacillus subtilis is not less than 1x108cfu/ml, Bacillus coagulans not less than 1x108cfu/ml, bacillus licheniformis is not less than 1x108cfu/ml, Lactobacillus bulgaricus not less than 1x1010cfu/ml, enterococcus faecalis not less than 1x109cfu/ml。
The second object of the invention is achieved by: the preparation method of the microbial preparation for improving the intestinal environment of the poultry is characterized by comprising the following steps:
1) preparing a spore strain seed culture medium, a lactic acid bacteria seed culture medium and a yeast seed culture solution, and sterilizing at 121 ℃;
2) inoculating strains into a seed culture medium, wherein bacillus subtilis, bacillus coagulans, bacillus licheniformis and the like are inoculated into the bacillus strain seed culture medium in mass, and are cultured in a heat preservation box at the temperature of 30 ℃ for 18-24 hours; inoculating lactobacillus bulgaricus, enterococcus faecalis, etc. in lactobacillus seed culture medium, and standing at 37 deg.C for 18-24 hr; inoculating Candida utilis activating bacteria in yeast seed liquid, and anaerobic culturing at 37 deg.c for 1-2 days;
3) preparing a spore bacteria fermentation culture medium, sterilizing at 121 ℃, preparing a lactic acid bacteria fermentation culture medium, sterilizing at 121 ℃, fermenting a yeast fermentation culture medium, and sterilizing at 121 ℃;
4) pouring the spore strain seed culture solution into a spore strain fermentation culture medium, and fermenting for 24 hours at 30 ℃; pouring the lactobacillus seed culture solution into a lactobacillus fermentation culture medium, and standing for 24 hours; pouring the yeast seed liquid into yeast fermentation culture medium, and fermenting at 30 deg.C for 1-2 days;
5) and after the fermentation is finished, mixing the culture solution, adding the phaffia rhodozyma to produce the astaxanthin, and subpackaging for later use.
In the scheme, the method comprises the following steps: the spore bacterium seed culture medium comprises the following components in percentage by mass: 0.3% of beef extract powder, 1% of peptone, 0.5% of sodium chloride and the balance of water.
In the scheme, the method comprises the following steps: the lactobacillus seed culture medium comprises the following components in percentage by mass: peptone 1%, beef extract 1%, yeast extract 0.5%, diammonium hydrogen citrate 0.2%, glucose 2%, magnesium sulfate 0.05%, manganese sulfate 0.025%, calcium carbonate 0.5%, and water in balance.
In the scheme, the method comprises the following steps: the spore bacterium fermentation medium comprises the following components in percentage by weight: 0.2% of glucose, 0.16% of peptone, 0.1% of yeast extract powder, 0.1% of sodium chloride and the balance of water.
In the scheme, the method comprises the following steps: the lactobacillus fermentation medium comprises the following components in percentage by weight: 3% of brown sugar, 1% of bean pulp, 1% of yeast powder, 0.05% of magnesium sulfate, 0.05% of manganese sulfate, 0.5% of sodium acetate, 0.2% of dipotassium hydrogen phosphate and the balance of water.
In the scheme, the method comprises the following steps: the yeast seed culture solution comprises the following components in percentage by weight: 20% of potatoes, 2% of glucose and the balance of water.
In the scheme, the method comprises the following steps: the yeast fermentation medium comprises the following components in percentage by weight: 2% of glucose, 1% of peptone and the balance of water.
In the scheme, the method comprises the following steps: during fermentation, the pH value of the yeast fermentation liquid is not required to be adjusted, the pH value of the spore bacteria fermentation liquid is kept at 7.0, and the pH value of the lactobacillus fermentation liquid is kept at about 6.5 by supplementing ammonia water.
The order of the bacteria colonization in the digestive tract is as follows: aerobic bacteria → facultative anaerobes → obligate anaerobes. The final colonization of obligate anaerobes is due to the fact that the environment is rich in oxygen, and only after the aerobic bacteria and the facultative anaerobes grow for a period of time, the oxygen in the surrounding environment is consumed in a large amount, so that conditions for the growth and colonization of the anaerobes can be created. The microbial preparation of the invention has the synergistic effect of aerobic bacteria, facultative anaerobic bacteria and obligate anaerobic bacteria, so that the anaerobic bacteria in the rear end of the large intestine always have absolute advantages in quantity. The microorganisms in the whole intestinal tract are in dynamic balance, and the organism can always maintain a normal physiological state. Finally, after the beneficial microorganisms enter the intestinal tract, the beneficial microorganisms can rapidly form dominant microbial flora together with beneficial bacteria in the intestinal tract, inhibit the growth of harmful bacteria in the intestinal tract, prevent the colonization of the harmful bacteria and adjust the microecological balance of the intestinal tract. The astaxanthin produced by the phaffia rhodozyma has extremely strong oxidation resistance, has biological functions of inhibiting tumorigenesis, enhancing immunologic function and the like, can regulate and control the free radical metabolism of oxidative stress animals, and reduces the damage of poultry to the intestinal tract of the poultry in a stress state. The bacillus subtilis can quickly consume the oxygen content in the intestinal tract, promote the proliferation of beneficial bacteria such as lactic acid bacteria and the like, the lactic acid bacteria can generate a large amount of lactic acid to inhibit the growth of pathogenic bacteria, enterococcus faecalis can be reactivated in the intestinal tract quickly to produce acid and enzyme, and the intestinal microecological balance is quickly established. Candida utilis contains abundant proteins, lipids, sugars, various vitamins and the like, and can produce enzymes, coenzymes, ribonucleic acids, sterols and the like and some metabolic intermediates in the fermentation process, so that the Candida utilis is widely applied to animal husbandry. The probiotics of the invention improve the production performance of the poultry and disease resistance by improving the micro-ecological environment of the intestinal canal of the poultry and improving the immunity of the poultry, and have no residue in poultry products, thereby having good economic benefit and ecological benefit.
Detailed Description
The invention is further illustrated by the following examples:
example 1
The preparation method of the microbial preparation for improving the intestinal environment of the poultry comprises the following steps:
the spore bacterium seed culture medium comprises, by mass, 0.3% of beef extract powder, 1% of peptone, 0.5% of sodium chloride, and the balance water.
The lactobacillus seed culture medium comprises the following components in percentage by mass: peptone 1%, beef extract 1%, yeast extract 0.5%, diammonium hydrogen citrate 0.2%, glucose 2%, magnesium sulfate 0.05%, manganese sulfate 0.025%, calcium carbonate 0.5%, and water in balance.
The spore bacterium fermentation medium comprises the following components in percentage by weight: 0.2% of glucose, 0.16% of peptone, 0.1% of yeast extract powder, 0.1% of sodium chloride and the balance of water.
The lactobacillus fermentation medium comprises the following components in percentage by weight: 3% of brown sugar, 1% of bean pulp, 1% of yeast powder, 0.05% of magnesium sulfate, 0.05% of manganese sulfate, 0.5% of sodium acetate, 0.2% of dipotassium hydrogen phosphate and the balance of water.
The yeast seed culture solution comprises the following components in percentage by weight: 20% of potatoes, 2% of glucose and the balance of water.
The yeast fermentation medium comprises the following components in percentage by weight: 2% of glucose, 1% of peptone and the balance of water.
1) 6L of spore strain seed culture medium, 10L of lactobacillus seed culture medium and 6L of yeast seed culture solution are prepared and sterilized for 15 minutes at 121 ℃.
2) Inoculating strains into a seed culture medium, wherein bacillus subtilis, bacillus coagulans, bacillus licheniformis and the like are inoculated into the bacillus strain seed culture medium in mass, and are cultured in an incubator at 30 ℃ and 150RPM for 18-24 hours; inoculating lactobacillus bulgaricus, enterococcus faecalis, etc. in lactobacillus seed culture medium, and standing at 37 deg.C for 18-24 hr; the Candida utilis activating bacteria are inoculated in yeast seed culture solution and anaerobically cultured at 37 deg.c for 1-2 days.
3) Preparing 250 liters of spore bacteria fermentation culture medium, sterilizing for 15 minutes at 121 ℃, preparing 500 liters of lactic acid bacteria fermentation culture medium, sterilizing for 15 minutes at 121 ℃, preparing 250 liters of yeast fermentation culture medium, and sterilizing for 15 minutes at 121 ℃.
4) Pouring the spore strain seed culture solution into a spore strain fermentation culture medium, fermenting at 30 ℃ for 24 hours, and controlling the pH value to be 7.0. Pouring the lactobacillus seed culture solution into a lactobacillus fermentation culture medium, standing and culturing for 24 hours, and keeping the pH value at about 6.5. The yeast seed liquid is poured into yeast fermentation culture medium and fermented for 1-2 days at 30 ℃, without regulating and controlling pH.
5) After fermentation, the culture solution is mixed and subpackaged for standby use, wherein the culture solution contains 1mg/L astaxanthin produced by phaffia rhodozyma and 1x10 Candida utilis8cfu/ml, Bacillus subtilis 1x108cfu/ml, Bacillus coagulans 1X108cfu/ml, Bacillus licheniformis 1x108cfu/ml, Lactobacillus bulgaricus 1x1010cfu/ml, enterococcus faecalis 1x109cfu/ml。
Example 2
The other steps are the same as example 1, the astaxanthin content of the yeast phaffia rhodozyma is 4mg/L, the Candida utilis content is not less than 1.2x108cfu/ml, Bacillus subtilis no less than 1.2x108cfu/ml, Bacillus coagulans not less than 1.2x108cfu/ml, Bacillus licheniformis is not less than 1.2x108cfu/ml, Lactobacillus bulgaricus not less than 1.2x1010cfu/ml, enterococcus faecalis not less than 1.2x109cfu/ml。
Example 3
Other examples were conducted in the same manner as example 1 except that the shrimp-producing strain contains Phaffia rhodozyma2mg/L of anthocyanin and not less than 1.15x10 of candida utilis8cfu/ml, bacillus subtilis not less than 1.15x108cfu/ml, Bacillus coagulans not less than 1.15x108cfu/ml, bacillus licheniformis is not less than 1.15x108cfu/ml, Lactobacillus bulgaricus not less than 1.15x1010cfu/ml, enterococcus faecalis not less than 1.15x109cfu/ml。
Application examples
About 50g of young broilers 300 are selected for experiments, randomly divided into 5 groups of 20 broilers, and three broilers in each group are fed: basic ration (formulated according to nutritional ingredients required for chicken growth), basic ration + 3% of probiotics according to the invention in example 1, basic ration + 3% of probiotics according to the invention in example 2, basic ration + neomycin sulfate (mixed drinking, 50-70mg per liter of water), and feeding time 45 d. And the growth was recorded, the results of which are shown in the following table:
the disease is mainly manifested as digestive tract diseases.
Therefore, the invention can reduce intestinal diseases of chickens, promote nutrient absorption, ensure the healthy growth of the broilers, and has good chicken quality and no residue.
The present invention is not limited to the above-described embodiments, and those skilled in the art will understand that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.
Claims (10)
1. A microbial preparation for improving the intestinal environment of poultry is characterized in that: comprises candida utilis, bacillus subtilis, bacillus coagulans, bacillus licheniformis, lactobacillus bulgaricus, enterococcus faecalis and metabolites thereof and phaffia rhodozyma astaxanthin.
2. The method of claim 1 for improving gut function in avianAn environmental microbial preparation characterized by: comprises 1-4mg/L astaxanthin produced by phaffia rhodozyma and 1x10 or more Candida utilis8cfu/ml, bacillus subtilis is not less than 1x108cfu/ml, Bacillus coagulans not less than 1x108cfu/ml, bacillus licheniformis is not less than 1x108cfu/ml, Lactobacillus bulgaricus not less than 1x1010cfu/ml, enterococcus faecalis not less than 1x109cfu/ml。
3. A method for preparing a microbial preparation for improving the intestinal environment of an avian according to any one of claims 1 to 2, comprising the steps of:
1) preparing a spore strain seed culture medium, a lactic acid bacteria seed culture medium and a yeast seed culture solution, and sterilizing at 121 ℃;
2) inoculating strains into a seed culture medium, wherein bacillus subtilis, bacillus coagulans, bacillus licheniformis and the like are inoculated into the bacillus strain seed culture medium in mass, and are cultured in a heat preservation box at the temperature of 30 ℃ for 18-24 hours; inoculating lactobacillus bulgaricus, enterococcus faecalis, etc. in lactobacillus seed culture medium, and standing at 37 deg.C for 18-24 hr; inoculating Candida utilis activating bacteria in yeast seed liquid, and anaerobic culturing at 37 deg.c for 1-2 days;
3) preparing a spore bacteria fermentation culture medium, sterilizing at 121 ℃, preparing a lactic acid bacteria fermentation culture medium, sterilizing at 121 ℃, fermenting a yeast fermentation culture medium, and sterilizing at 121 ℃;
4) pouring the spore strain seed culture solution into a spore strain fermentation culture medium, and fermenting for 24 hours at 30 ℃; pouring the lactobacillus seed culture solution into a lactobacillus fermentation culture medium, and standing for 24 hours; pouring the yeast seed liquid into yeast fermentation culture medium, and fermenting at 30 deg.C for 1-2 days;
5) and after the fermentation is finished, mixing the culture solution, adding the phaffia rhodozyma to produce the astaxanthin, and subpackaging for later use.
4. The method for preparing the microbial preparation for improving intestinal environment of poultry according to claim 3, wherein the microbial preparation comprises the following steps: the spore bacterium seed culture medium comprises the following components in percentage by mass: 0.3% of beef extract powder, 1% of peptone, 0.5% of sodium chloride and the balance of water.
5. The method for preparing the microbial preparation for improving intestinal environment of poultry according to claim 3, wherein the lactobacillus seed culture medium comprises the following components by mass percent: peptone 1%, beef extract 1%, yeast extract 0.5%, diammonium hydrogen citrate 0.2%, glucose 2%, magnesium sulfate 0.05%, manganese sulfate 0.025%, calcium carbonate 0.5%, and water in balance.
6. The method for preparing a microbial preparation for improving intestinal environment of poultry according to claim 3, wherein the spore bacteria fermentation medium comprises the following components by weight percent: 0.2% of glucose, 0.16% of peptone, 0.1% of yeast extract powder, 0.1% of sodium chloride and the balance of water.
7. The method for preparing a microbial preparation for improving intestinal environment of poultry according to claim 3, wherein the lactobacillus fermentation medium comprises the following components by weight percent: 3% of brown sugar, 1% of bean pulp, 1% of yeast powder, 0.05% of magnesium sulfate, 0.05% of manganese sulfate, 0.5% of sodium acetate, 0.2% of dipotassium hydrogen phosphate and the balance of water.
8. The method for preparing a microbial preparation for improving intestinal environment of fowls according to claim 3, wherein the yeast seed culture solution comprises the following components in percentage by weight: 20% of potatoes, 2% of glucose and the balance of water.
9. The method for preparing a microbial preparation for improving intestinal environment of fowls according to claim 3, wherein the yeast fermentation medium comprises the following components in percentage by weight: 2% of glucose, 1% of peptone and the balance of water.
10. The process for preparing a microbial preparation for improving the intestinal environment of poultry according to any one of claims 3 to 9, wherein: during fermentation, the pH value of the yeast fermentation liquid is not required to be adjusted, the pH value of the spore bacteria fermentation liquid is kept at 7.0, and the pH value of the lactobacillus fermentation liquid is kept at about 6.5 by supplementing ammonia water.
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| CN113897299A (en) * | 2021-10-19 | 2022-01-07 | 武安市益微益生物科技有限公司 | Composite microecological preparation and application thereof |
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| CN103060247A (en) * | 2013-01-25 | 2013-04-24 | 赵颖 | Probiotics microbial agent for regulating livestock intestinal flora and preparation method thereof |
| CN105985916A (en) * | 2015-01-28 | 2016-10-05 | 河南惠通天下动物药业有限公司 | Composite microorganism preparation and preparation method thereof |
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| CN101803682A (en) * | 2009-12-30 | 2010-08-18 | 沈阳科丰牧业科技有限公司 | Compound microbial agent and production method and application thereof |
| CN103060247A (en) * | 2013-01-25 | 2013-04-24 | 赵颖 | Probiotics microbial agent for regulating livestock intestinal flora and preparation method thereof |
| CN105985916A (en) * | 2015-01-28 | 2016-10-05 | 河南惠通天下动物药业有限公司 | Composite microorganism preparation and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113897299A (en) * | 2021-10-19 | 2022-01-07 | 武安市益微益生物科技有限公司 | Composite microecological preparation and application thereof |
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