CN115005159A - Breeding method for maintaining intestinal health of piglets and application of breeding method - Google Patents
Breeding method for maintaining intestinal health of piglets and application of breeding method Download PDFInfo
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- CN115005159A CN115005159A CN202210672530.5A CN202210672530A CN115005159A CN 115005159 A CN115005159 A CN 115005159A CN 202210672530 A CN202210672530 A CN 202210672530A CN 115005159 A CN115005159 A CN 115005159A
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- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 230000037356 lipid metabolism Effects 0.000 description 1
- 210000005228 liver tissue Anatomy 0.000 description 1
- 150000004668 long chain fatty acids Chemical class 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229940057917 medium chain triglycerides Drugs 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229960005010 orotic acid Drugs 0.000 description 1
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 210000003240 portal vein Anatomy 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- HKEAFJYKMMKDOR-VPRICQMDSA-N puerarin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=CC(C2=O)=C1OC=C2C1=CC=C(O)C=C1 HKEAFJYKMMKDOR-VPRICQMDSA-N 0.000 description 1
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 1
- 238000002407 reforming Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000011506 response to oxidative stress Effects 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 229940088197 riboflavin 3 mg Drugs 0.000 description 1
- 150000004671 saturated fatty acids Chemical class 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 150000004666 short chain fatty acids Chemical class 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 235000019163 vitamin B12 Nutrition 0.000 description 1
- 239000011715 vitamin B12 Substances 0.000 description 1
- 235000019158 vitamin B6 Nutrition 0.000 description 1
- 239000011726 vitamin B6 Substances 0.000 description 1
- QYSXJUFSXHHAJI-YRZJJWOYSA-N vitamin D3 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C\C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-YRZJJWOYSA-N 0.000 description 1
- 235000005282 vitamin D3 Nutrition 0.000 description 1
- 239000011647 vitamin D3 Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 235000019168 vitamin K Nutrition 0.000 description 1
- 239000011712 vitamin K Substances 0.000 description 1
- 150000003721 vitamin K derivatives Chemical class 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
- 229940021056 vitamin d3 Drugs 0.000 description 1
- 229940046010 vitamin k Drugs 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
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- Botany (AREA)
- Environmental Sciences (AREA)
- Sustainable Development (AREA)
- Animal Behavior & Ethology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention relates to a vitaminThe breeding method for protecting the intestinal health of the piglets comprises the following steps: feeding special feed for piglets: the special feed for the piglets comprises a base material, a fat compound, a fermentation compound and a premix; feeding mode: intermittent feeding limitation; and (3) environmental regulation: controlling temperature, humidity and CO in pigsty 2 Concentration and NH 3 And (4) concentration. The breeding method for promoting the intestinal health of the piglets organically combines the feeding mode, the environmental temperature, the air quality and the nutrition regulation, and a more reasonable and scientific feeding management method is prepared, so that the metabolism of organisms can be effectively promoted, pathogenic bacteria can be effectively killed or prefabricated, free radicals in the bodies can be eliminated, inflammation and oxidation can be prevented, energy and essential fatty acid can be effectively provided for the intestinal tracts, the intestinal flora can be regulated, and the intestinal health can be maintained.
Description
Technical Field
The invention belongs to the technical field of pig breeding, relates to a breeding method for maintaining intestinal health of piglets and application thereof, and particularly relates to a breeding method for adjusting intestinal flora, maintaining intestinal health and improving piglet breeding benefit and application thereof.
Background
In order to improve the breeding benefit, the genetic improvement of the pigs always focuses on improving the growth speed of the pigs and obtains certain improvement effect. However, as the digestive system of the piglets is not developed completely, the intestinal flora is not established completely, and the nutrition can not be absorbed effectively, the intestinal injury of the piglets is caused by the long-term high-load nutrition intake, so that the health of the piglets is affected. Meanwhile, external factors such as weaning, diseases, high temperature, feed mildew and the like can cause a large amount of free radicals to be generated in the bodies of the piglets to induce oxidative stress reaction. Oxidative stress breaks the redox balance state of animal intestinal tracts and influences the content and activity of oxidase secreted by intestinal mucosa. The intestinal tract of weaned pigs is incompletely developed, the stress resistance of the organism is weak, and when the weaned pigs are subjected to oxidative stress, the barrier function of the intestinal tract is easily damaged, so that the secretion of digestive enzymes in the intestinal tract is insufficient, and the digestion and absorption of nutrient substances are influenced.
The intestinal tract is the main place for digesting and absorbing nutrient substances, is also a natural physiological barrier for preventing external harmful substances from entering animal bodies, and is an important immune organ of a body defense system. In the growth stage of young piglets, the intestinal tracts of the piglets are easily attacked by harmful substances, pathogenic bacteria and the like, so that oxidative stress injury is caused, the barrier function of the intestinal tracts is disordered, and the piglets have poor appetite and slow growth.
The factors influencing the intestinal health of the piglets are many and comprise heredity, nutrition, feeding and the like. Among them, nutrition is the most studied as an important influencing factor at present. For example, CN102870993A discloses a compound feed additive for effectively improving the intestinal health of pigs, the feed can achieve good diarrhea resistance through scientific compounding, simultaneously effectively improve the immunity of piglets, reduce the use of antibiotic products, improve the growth quality of the piglets, and lay a foundation for realizing the production potential of the piglets; meanwhile, the phenomena of hair growth of piglets in the nursery stage and growth and development obstruction after the nursery stage are avoided; the environmental pollution is reduced.
However, in the research of piglets, the feeding management of piglets is not paid enough attention, and more, the feeding management and the breeding technology are neglected aiming at the improvement of nutrition on the growth of piglets, so that the breeding benefit of the piglets is not expected.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a breeding method for maintaining the intestinal health of piglets and application thereof, and particularly relates to a breeding method for adjusting intestinal flora, maintaining the intestinal health and improving the breeding benefit of piglets and application thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the present invention provides a breeding method for maintaining intestinal health of piglets, comprising:
feeding special feed for piglets: the special feed for the piglets comprises a base material, a fat compound, a fermentation compound and a premix;
feeding mode: intermittent feeding limitation;
and (3) environmental regulation: controlling temperature, humidity and CO in pigsty 2 Concentration and NH 3 And (4) concentration.
The breeding method for promoting the intestinal health of the piglets organically combines the feeding mode, the environmental temperature, the air quality and the nutrition regulation, and a more reasonable and scientific feeding management method is prepared, so that the metabolism of organisms can be effectively promoted, pathogenic bacteria can be effectively killed or prefabricated, free radicals in the bodies can be eliminated, inflammation and oxidation can be prevented, energy and essential fatty acid can be effectively provided for the intestinal tracts, the intestinal flora can be regulated, and the intestinal health can be maintained.
In order to pursue the maximum growth speed, the piglets in the current farm mainly adopt free feeding, but the intestinal tracts of the piglets can be in a high-load state for a long time by adopting the long-term free feeding, so that the absorption of the intestinal tracts is influenced.
The invention can provide optimum environment for piglets by regulating and controlling the environmental temperature, humidity and air quality of the pigsty, reduce the influence of environmental pathogenic factors on the immune system of the piglets and promote the development and maturity of the digestive system of the piglets.
The feed formula provided by the invention is characterized in that a fat compound and a fermentation compound are further added into a base material, wherein the fat compound is used as an energy supply substance to provide energy and essential fatty acid for intestinal tracts, so that the energy can be rapidly provided for the intestinal tracts of piglets, the intestinal villus recovery and growth are promoted, and the intestinal health is maintained; the fermented compound is rich in various bioactive substances and high-quality fibers after being fermented, can obviously improve the micro-ecological environment of intestinal tracts of organisms, can effectively increase the growth of beneficial bacteria, reduce the field planting of harmful bacteria, protect intestinal mucosa barriers, and avoid the problems of intestinal metabolic dysfunction, intestinal injury and the like.
Preferably, the intermittent feeding limitation mode is as follows: 3-7 days (e.g., 3 days, 4 days, 5 days, 6 days, 7 days) are fed freely, and 1 day is fed to 70-80% (e.g., 70%, 72%, 75%, 78%, 80%, etc.) of the free food intake.
The intermittent feeding limiting mode can be selected to effectively regulate the metabolism of the organism, relieve the intestinal burden of piglets and maintain the intestinal health.
Preferably, the feeding restriction mode is timed feeding, and feeding is respectively carried out once in the morning, the middle and the evening, for example, feeding is carried out once at about 8:00, about 14:00 and about 20: 00.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
Preferably, the temperature in the pigsty is controlled to be 22-26 ℃, such as 22 ℃, 23 ℃, 24 ℃, 25 ℃, 26 ℃ and the like; the humidity is controlled at 60-70%, such as 60%, 62%, 64%, 65%, 67%, 68%, 70%, etc.
Preferably, the CO in the pig house 2 The concentration is controlled at 1500- 3 For example 1500mg/m 3 、1600mg/m 3 、1700mg/m 3 、1800mg/m 3 、1900mg/m 3 、2000mg/m 3 Etc.; NH (NH) 3 The concentration is controlled at 0.2-0.6mg/m 3 For example 0.2mg/m 3 、0.3mg/m 3 、0.4mg/m 3 、0.5mg/m 3 、0.6mg/m 3 And the like.
Other specific values within the above ranges can be selected, and are not described in detail herein.
Selecting the above specific pigsty temperature, humidity and CO 2 Concentration, NH 3 The concentration regulation and control numerical range can better promote the development and maturity of the digestive system of the piglets, thereby promoting the intestinal flora balance and the intestinal health of the piglets.
Preferably, the fat complex comprises antarctic krill phospholipids, an OMO structural fat, inulin butyrate, a coated plant essential oil, an amino acid, and a carrier.
The phospholipid is characterized in that saturated fatty acid is connected to the sn-1 position of a glycerol skeleton of the phospholipid of Antarctic krill, unsaturated fatty acid is connected to the sn-2 position of the glycerol skeleton, the phospholipid is stable in oxidability, is rich in n-3PUFAs, and PUFAs such as EPA, DHA and DPA are concentrated on the sn-2 position of the glycerol skeleton and are fatty acid indispensable for maintaining the homeostasis of piglets. In addition, the antarctic krill phospholipid can also improve the activity of intrahepatic lipid acyltransferase, regulate lipid metabolism and synthesis genes, has an anti-inflammatory effect, and maintains the intestinal health of piglets.
The OMO structural ester is 1, 3-dioleic acid-2-medium chain fatty acid (OMO) structural ester, is symmetrical medium-long chain triglyceride containing oleic acid at sn-1 and 3 positions and medium-chain fatty acid at sn-2 positions, and is a novel structural ester similar to human milk structural ester OPO. The unique fatty acid profile facilitates nutrient absorption, medium chain fatty acids are more readily digested and metabolized in the body than long chain fatty acids, and in particular, medium chain fatty acids in the form of sn-2 monoglycerides can be esterified to triglycerides without re-esterificationAnd then absorbing. It has the advantages that oleic acid can be effectively absorbed after the sn-1 and 3 positions are hydrolyzed, and medium-chain fatty acid can rapidly provide energy on the sn-2 position. The metabolism of medium chain triglyceride and long chain triglyceride in vivo is different, and the long chain triglyceride is decomposed and transported to mucous membrane cells through lymph to form new triglyceride; the medium chain triglycerides are hydrolyzed by lipase to medium chain fatty acids, enter the liver from the portal vein, and are oxidized to H in the liver 2 O、CO 2 And energy or carcass formation, not readily storable in adipocytes or liver tissue. The OMO structural ester combines the advantages of the OMO structural ester and the OMO structural ester in vivo and can overcome the defects, and medium-chain fatty acid combined on the sn-2 position of the OMO structural ester can rapidly provide energy and avoid reforming triglyceride.
The inulin butyrate can improve the defect of directly supplementing short-chain fatty acid (butyric acid), and can convey the butyric acid to the intestinal tract to promote the growth and development of intestinal villi of piglets. Inulin is not easy to digest in oral cavity, stomach and small intestine, and can only reach colon and be fermented under the action of a large amount of beneficial bacteria to generate SCFA, so that the supplement of inulin can obviously promote the proliferation of intestinal probiotics such as bifidobacterium, lactobacillus and the like, and the fermentation of generated SCFA under the action of intestinal microorganisms can improve intestinal flora, promote the growth and development of intestinal villus of piglets, absorb mineral substances and the like.
Wherein the plant essential oil and amino acid can assist in promoting fat metabolism and further promote intestinal health.
Preferably, the fat complex comprises, by weight, 10-20 parts of antarctic krill phospholipid, 20-40 parts of OMO structural lipid, 5-10 parts of inulin butyrate, 0.1-1 part of coated plant essential oil, 5-15 parts of amino acid and 30-60 parts of carrier based on the total weight of the fat complex.
The weight parts of the antarctic krill phospholipid can be selected from 10 parts, 12 parts, 15 parts, 18 parts, 20 parts and the like.
The weight parts of the inulin butyrate can be selected from 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts and the like.
The parts by weight of the OMO structural grease can be selected from 20 parts, 23 parts, 25 parts, 30 parts, 32 parts, 35 parts, 38 parts, 40 parts and the like.
The coating plant essential oil can be selected from 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 1 part and the like in parts by weight.
The amino acid can be selected from 5 parts, 6 parts, 7 parts, 8 parts, 10 parts, 12 parts, 13 parts, 14 parts, 15 parts and the like.
The weight parts of the carrier can be selected from 30 parts, 35 parts, 38 parts, 40 parts, 42 parts, 45 parts, 48 parts, 50 parts, 52 parts, 55 parts, 58 parts, 60 parts and the like.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
Preferably, the medium-chain fatty acid in the OMO structural lipid comprises caprylic acid, capric acid, lauric acid and myristic acid, and the mass ratio of caprylic acid, capric acid, lauric acid and myristic acid is (1-3): (1-5): (1-2): (3-5).
Preferably, the coated plant essential oil comprises anise essential oil and capsicum essential oil.
Preferably, the amino acids include leucine and valine.
Preferably, the carrier comprises puffed corn flour and/or puffed corncob flour.
Preferably, the fat complex is prepared by a preparation method comprising the following steps:
heating and dissolving Antarctic krill phospholipid and OMO structure lipid, mixing with inulin butyrate and amino acid, mixing with carrier, adsorbing, cooling, and mixing with coated plant essential oil.
Preferably, the heating means heating to 60-100 ℃, such as 60 ℃, 65 ℃, 70 ℃, 75 ℃, 80 ℃, 85 ℃, 90 ℃, 95 ℃, 100 ℃ etc.
Preferably, the cooling means cooling to 15-40 ℃, such as 15 ℃, 20 ℃, 25 ℃, 30 ℃, 35 ℃, 40 ℃ and the like.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
Preferably, the fermentation complex is a product obtained by microbial fermentation of a fermentation feedstock;
the fermentation raw material comprises at least two of soybean flour, antarctic krill powder, moringa oleifera leaf powder, radix puerariae powder, jerusalem artichoke powder and macleaya cordata leaf powder.
Preferably, the fermentation raw material comprises a combination of soybean meal, antarctic krill meal, moringa oleifera leaf meal, pueraria lobata meal, jerusalem artichoke meal and macleaya cordata leaf meal.
The moringa leaf powder is rich in moringa lignin and has antibacterial and anti-inflammatory effects; the radix Puerariae powder is rich in puerarin, can directly remove free radicals in organism, and has antiinflammatory and antioxidant effects; the jerusalem artichoke powder is rich in inulin and water-soluble dietary fiber, can hardly be hydrolyzed and digested by gastric acid, and is only utilized by beneficial microorganisms in colon, so that the intestinal environment is improved; the Macleaya cordata leaf powder contains alkaloid as main active component, and has antibacterial and anti-inflammatory effects.
Preferably, the fermentation raw material comprises the following components in parts by weight based on the total weight of the fermentation raw material: 10-30 parts of soybean meal, 1-10 parts of antarctic krill powder, 30-50 parts of moringa oleifera leaf powder, 20-40 parts of radix puerariae powder, 5-10 parts of jerusalem artichoke powder and 10-30 parts of macleaya cordata leaf powder.
When the fermentation raw materials are combined in the specific mode, the growth of beneficial bacteria is increased, the planting of harmful bacteria is reduced, the intestinal mucosa barrier is protected, and the effects of avoiding intestinal metabolic dysfunction and intestinal injury are more obvious.
The soybean powder can be selected from 10 parts, 12 parts, 15 parts, 18 parts, 20 parts, 22 parts, 25 parts, 28 parts, 30 parts and the like.
The weight parts of the antarctic krill powder can be selected from 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 7 parts, 8 parts, 9 parts, 10 parts and the like.
The moringa leaf powder can be selected from 30 parts, 32 parts, 35 parts, 38 parts, 40 parts, 42 parts, 45 parts, 48 parts, 50 parts and the like in parts by weight.
The kudzu root powder can be selected from 20 parts, 22 parts, 25 parts, 28 parts, 30 parts, 32 parts, 35 parts, 38 parts, 40 parts and the like.
The weight parts of the jerusalem artichoke powder can be selected from 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts and the like.
The macleaya cordata leaf powder can be selected from 10 parts, 12 parts, 15 parts, 18 parts, 20 parts, 22 parts, 25 parts, 28 parts, 30 parts and the like.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
Preferably, the microorganism comprises a combination of enterococcus faecalis, bacillus subtilis and aspergillus oryzae.
Preferably, the microorganism comprises 10-30 parts of enterococcus faecalis, 20-40 parts of bacillus subtilis and 20-40 parts of aspergillus oryzae in parts by weight based on the total weight of the microorganism.
The invention creatively carries out the combined fermentation treatment of bacteria and mold, and selects the three specific strains, and the effect of the invention on the aspect of regulating the intestinal health is more excellent.
The weight parts of the enterococcus faecalis can be selected from 10 parts, 12 parts, 15 parts, 18 parts, 20 parts, 22 parts, 25 parts, 28 parts, 30 parts and the like.
The weight parts of the bacillus subtilis can be selected from 20 parts, 22 parts, 25 parts, 28 parts, 30 parts, 32 parts, 35 parts, 38 parts, 40 parts and the like.
The Aspergillus oryzae can be selected from 20 parts, 22 parts, 25 parts, 28 parts, 30 parts, 32 parts, 35 parts, 38 parts, 40 parts and the like.
Preferably, the total weight of the microorganism is 5-10% of the total weight of the fermentation feedstock, e.g., 5%, 6%, 7%, 8%, 9%, 10%, etc.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
Preferably, the fermentation complex is prepared by the following method:
mixing the fermentation raw materials with bacillus subtilis and aspergillus oryzae for aerobic fermentation, then mixing with enterococcus faecalis for anaerobic fermentation, and drying to obtain the product.
Preferably, the aerobic fermentation conditions are: the fermentation temperature is 25-40 deg.C (such as 25 deg.C, 30 deg.C, 32 deg.C, 33 deg.C, 35 deg.C, 37 deg.C, 38 deg.C, 40 deg.C), the water content is 30-45% (such as 30%, 32%, 35%, 38%, 40%, 43%, 45%, etc.), and the fermentation time is 24-72h (such as 24h, 48h, 52h, 55h, 60h, 70h, 72h, etc.).
Preferably, the anaerobic fermentation conditions are: the fermentation temperature is 25-40 deg.C (such as 25 deg.C, 30 deg.C, 32 deg.C, 33 deg.C, 35 deg.C, 37 deg.C, 38 deg.C, 40 deg.C), the water content is 30-45% (such as 30%, 32%, 35%, 38%, 40%, 43%, 45%, etc.), and the fermentation time is 48-96h (such as 48h, 52h, 55h, 60h, 70h, 80h, 90h, 96h, etc.).
The fermentation compound is prepared by the specific fermentation process, and has a more remarkable effect in promoting the intestinal health of piglets.
Preferably, the base material comprises corn.
Preferably, the premix comprises a vitamin premix and a trace element premix.
Preferably, the vitamin premix comprises any one or a combination of at least two of vitamin a, vitamin D3, vitamin E, vitamin K, vitamin B1, vitamin B6, vitamin B12, biotin, choline, riboflavin, folic acid, or nicotinic acid.
Preferably, the trace element premix comprises any one or a combination of at least two of I, Fe, Mn, Se, or Zn.
Preferably, the preparation method of the special feed for piglets comprises the following steps:
mixing the base material, the fat compound, the fermentation compound and the premix uniformly.
In a second aspect, the invention provides the use of the breeding method for maintaining the intestinal health of piglets according to the first aspect in improving the breeding benefits of the piglets.
In a third aspect, the invention provides a feed for maintaining intestinal health of piglets, which comprises a base material, a fat compound, a fermentation compound and a premix.
Preferably, the fat complex comprises antarctic krill phospholipids, an OMO structural lipid, inulin butyrate, a coated plant essential oil, an amino acid, and a carrier.
Preferably, the fat complex comprises 10-20 parts of antarctic krill phospholipid, 20-40 parts of OMO structural lipid, 5-10 parts of inulin butyrate, 0.1-1 part of coated vegetable essential oil, 5-15 parts of amino acid and 30-60 parts of carrier in parts by weight based on the total weight of the fat complex.
Preferably, the coated plant essential oil comprises anise essential oil and capsicum essential oil.
Preferably, the amino acids include leucine and valine.
Preferably, the carrier comprises puffed corn flour and/or puffed corncob flour.
Preferably, the fat complex is prepared by a preparation method comprising the following steps:
heating and dissolving Antarctic krill phospholipid and OMO structure lipid, mixing with inulin butyrate and amino acid, mixing with carrier, adsorbing, cooling, and mixing with coated plant essential oil.
Preferably, the fermentation complex is a product obtained by microbial fermentation of a fermentation feedstock;
the fermentation raw material comprises at least two of soybean flour, antarctic krill powder, moringa oleifera leaf powder, radix puerariae powder, jerusalem artichoke powder and macleaya cordata leaf powder.
Preferably, the fermentation raw material comprises a combination of soybean meal, antarctic krill meal, moringa oleifera leaf meal, pueraria lobata meal, jerusalem artichoke meal and macleaya cordata leaf meal.
Preferably, the fermentation raw material comprises the following components in parts by weight based on the total weight of the fermentation raw material: 10-30 parts of soybean meal, 1-10 parts of antarctic krill powder, 30-50 parts of moringa oleifera leaf powder, 20-40 parts of radix puerariae powder, 5-10 parts of jerusalem artichoke powder and 10-30 parts of macleaya cordata leaf powder.
Preferably, the microorganism comprises a combination of enterococcus faecalis, bacillus subtilis and aspergillus oryzae.
Preferably, the microorganism comprises 10-30 parts of enterococcus faecalis, 20-40 parts of bacillus subtilis and 20-40 parts of aspergillus oryzae in parts by weight based on the total weight of the microorganism.
Preferably, the total weight of the microorganism is 5-10% of the total weight of the fermentation feedstock.
Preferably, the fermentation complex is prepared by the following method:
mixing the fermentation raw materials with bacillus subtilis and aspergillus oryzae for aerobic fermentation, then mixing with enterococcus faecalis for anaerobic fermentation, and drying to obtain the product.
Compared with the prior art, the invention has the following beneficial effects:
the breeding method for promoting the intestinal health of the piglets organically combines the feeding mode, the environmental temperature, the air quality and the nutrition regulation, and a more reasonable and scientific feeding management method is prepared, so that the metabolism of organisms can be effectively promoted, pathogenic bacteria can be effectively killed or prefabricated, free radicals in the bodies can be eliminated, inflammation and oxidation can be prevented, energy and essential fatty acid can be effectively provided for the intestinal tracts, the intestinal flora can be regulated, and the intestinal health can be maintained.
The intermittent feeding limiting mode adopted by the invention can regulate the metabolism of the organism, relieve the intestinal burden of piglets and maintain the intestinal health. The invention can provide optimum environment for piglets by regulating and controlling the environmental temperature, humidity and air quality of the pigsty, reduce the influence of environmental pathogenic factors on the immune system of the piglets and promote the development and maturity of the digestive system of the piglets. The feed formula provided by the invention is characterized in that a fat compound and a fermentation compound are further added into a base material, wherein the fat compound is used as an energy supply substance to provide energy and essential fatty acid for intestinal tracts, so that the energy can be rapidly provided for the intestinal tracts of piglets, the intestinal villus recovery and growth are promoted, and the intestinal health is maintained; the fermented compound is rich in various bioactive substances and high-quality fibers after being fermented, can obviously improve the micro-ecological environment of intestinal tracts of organisms, can effectively increase the growth of beneficial bacteria, reduce the field planting of harmful bacteria, protect intestinal mucosa barriers, and avoid the problems of intestinal metabolic dysfunction, intestinal injury and the like.
Detailed Description
To further illustrate the technical means and effects of the present invention, the following further describes the technical solution of the present invention with reference to the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.
The raw materials for the following preparations and examples and comparative examples are commercially available or obtained by conventional techniques known to those skilled in the art through conventional disclosures and common general knowledge.
The viable count of the enterococcus faecalis raw material related to the following preparation example was 10 11 CFU/g, the viable count in the raw material of the bacillus subtilis is 10 11 The number of spores in raw materials of CFU/g and Aspergillus oryzae is 2 multiplied by 10 10 CFU/g。
Antarctic krill phospholipids are products purchased from Sievast Biotechnology GmbH.
Inulin butyrate is a product available from Biotech, Inc., a source of North river.
The star anise essential oil is a product purchased from the biological technology company Limited of Dufu, Qingdao.
The capsicum essential oil is a product purchased from Qingdao Aidifu biotechnology limited.
The moringa oleifera leaf powder is a product purchased from western safe Olympic Biotech limited.
The radix Puerariae powder is a product purchased from Shanxi Fuzhu Biotech limited.
Jerusalem artichoke powder is a product purchased from Sierra's bioengineering Co.
The Macleaya cordata leaf powder is a product purchased from Hibiscus Biotech Co.
Preparation example 1
The preparation example provides a special feed for piglets, which is prepared by uniformly mixing 50 parts of corn, 5 parts of fat compound, 40 parts of fermentation compound and 5 parts of premix in parts by weight;
the preparation method of the fat compound takes the total weight of the fat compound as a reference, and comprises the following steps: mixing 10 parts of Antarctic krill phospholipid, 30 parts of OMO structural lipid and 10 parts of inulin butyrate, heating to 70 ℃ for complete dissolution, mixing with 40 parts of puffed corn flour for adsorption, cooling to 25 ℃, and then uniformly mixing with 5 parts of leucine, 5 parts of valine, 0.5 part of anise essential oil and 0.5 part of pepper essential oil to obtain the composition; wherein the medium-chain fatty acid raw materials in the OMO structural fat comprise caprylic acid, capric acid, lauric acid and myristic acid in a mass ratio of 2:4:1: 4;
based on the total weight of the fermentation compound, the preparation method of the fermentation compound comprises the following steps: mixing 20 parts of soybean meal, 10 parts of antarctic krill powder, 40 parts of moringa oleifera leaf powder, 30 parts of kudzu root powder, 10 parts of jerusalem artichoke powder and 20 parts of macleaya cordata leaf powder with 30 parts of bacillus subtilis and 30 parts of aspergillus oryzae, carrying out aerobic fermentation at 35 ℃ for 48 hours, then mixing with 20 parts of enterococcus faecalis, carrying out anaerobic fermentation at 35 ℃ for 40 percent of water content, and carrying out fermentation for 72 hours; drying to obtain the product;
the premix comprises VA 15000IU/kg, VD 33000 IU/kg, VE 100mg/kg, VK 2mg/kg, vitamin B13 mg/kg, VB 61 mg/kg, VB 1230 mu g/kg, biotin 0.5mg/kg, choline 0.4g/kg, riboflavin 3mg/kg, folic acid 3mg/kg, nicotinic acid 20mg/kg, I0.3 mg/kg, Fe 80mg/kg, Mn 30mg/kg, Se 0.5mg/kg and Zn 60mg/kg in terms of concentration in the feed.
Preparation example 2
The preparation example provides a special feed for piglets, and the composition formula of the special feed is different from that of the preparation example 1 only in that the special feed is a fermentation raw material of a fermentation compound, and the special feed is composed of 50 parts of kudzuvine root powder, 17 parts of jerusalem artichoke powder and 33 parts of macleaya cordata leaf powder. The other raw materials and the preparation process were the same as in example 1.
Preparation example 3
The preparation example provides a special feed for piglets, which is only different from the preparation example 1 in the composition formula of a fermentation raw material of a fermentation compound and comprises 57 parts of moringa oleifera leaf powder, 14 parts of jerusalem artichoke powder and 29 parts of macleaya cordata leaf powder. The other raw materials and the preparation process were the same as in example 1.
Preparation example 4
The preparation example provides a special feed for piglets, and the composition formula of the special feed is different from that of the preparation example 1 only in that the special feed is a fermentation raw material of a fermentation compound, and the special feed comprises 44 parts of moringa oleifera leaf powder, 33 parts of pueraria lobata powder and 23 parts of macleaya cordata leaf powder. The other raw materials and the preparation process were the same as in example 1.
Preparation example 5
The preparation example provides a special feed for piglets, and the composition formula of the special feed is different from that of the preparation example 1 only in that the fermentation raw materials of the fermentation compound are different, and the special feed comprises 50 parts of moringa oleifera leaf powder, 37.5 parts of kudzu root powder and 12.5 parts of jerusalem artichoke powder. The other raw materials and the preparation process were the same as in example 1.
Preparation example 6
The preparation example provides a special feed for piglets, the composition formula of the special feed is different from that of the preparation example 1 only in the fermentation process of a fermentation compound, and the preparation method of the fermentation compound comprises the following steps: mixing 40 parts of moringa oleifera leaf powder, 30 parts of radix puerariae powder, 10 parts of jerusalem artichoke powder and 20 parts of macleaya cordata leaf powder with 60 parts of bacillus subtilis, carrying out aerobic fermentation at 35 ℃ for 48 hours of water content, then mixing with 20 parts of enterococcus faecalis, and carrying out anaerobic fermentation at 35 ℃ for 72 hours of water content 40%; and (5) drying to obtain the product. The other raw materials and the preparation process were the same as in example 1.
Preparation example 7
The preparation example provides a special feed for piglets, the composition formula of the special feed is different from that of the preparation example 1 only in the fermentation process of the fermentation compound, and the preparation method of the fermentation compound is as follows: mixing 40 parts of moringa leaf powder, 30 parts of kudzu root powder, 10 parts of jerusalem artichoke powder and 20 parts of macleaya cordata leaf powder with 60 parts of aspergillus oryzae, carrying out aerobic fermentation at 35 ℃ for 48 hours with the water content, then mixing with 20 parts of enterococcus faecalis, and carrying out anaerobic fermentation at 35 ℃ for 72 hours with the water content of 40%; and (5) drying to obtain the product. The other raw materials and the preparation process were the same as in example 1.
Preparation example 8
The preparation example provides a special feed for piglets, and the composition formula of the special feed is only different from that of the preparation example 1 in that the raw materials of the fat compound, namely 10 parts of Antarctic krill phospholipid, 30 parts of OMO structural fat and 10 parts of inulin butyrate, are replaced by 37.5 parts of OMO structural fat and 12.5 parts of inulin butyrate, and other raw materials and preparation processes are consistent with those of the preparation example 1.
Preparation example 9
The preparation example provides a special feed for piglets, and the difference between the composition formula of the special feed and the preparation example 1 is only that the preparation raw materials of the fat complex, namely 10 parts of Antarctic krill phospholipid, 30 parts of OMO structural fat and 10 parts of inulin butyrate are replaced by 25 parts of Antarctic krill phospholipid and 25 parts of inulin butyrate, and other raw materials and preparation processes are consistent with those in the example 1.
Preparation example 10
The preparation example provides a special feed for piglets, and the composition formula of the special feed is only different from that of the preparation example 1 in that the raw materials of the fat compound, namely 10 parts of Antarctic krill phospholipid, 30 parts of OMO structural fat and 10 parts of inulin butyrate, are replaced by 12.5 parts of Antarctic krill phospholipid and 37.5 parts of OMO structural fat, and other raw materials and preparation processes are consistent with those of the preparation example 1.
Preparation example 11
The preparation example provides a special feed for piglets, and the composition formula of the special feed is only different from that of the preparation example 1 in that the raw materials for preparing the fat compound, namely 0.5 part of star anise essential oil and 0.5 part of pepper essential oil, are replaced by 1 part of star anise essential oil, and other raw materials and preparation processes are consistent with those of the preparation example 1.
Preparation example 12
The preparation example provides a special feed for piglets, and the composition formula of the special feed is only different from that of the preparation example 1 in that the raw materials for preparing the fat compound, namely 0.5 part of anise essential oil and 0.5 part of pepper essential oil, are replaced by 1 part of pepper essential oil, and other raw materials and preparation processes are consistent with those in the example 1.
Example 1
The embodiment provides a breeding method for maintaining intestinal health of piglets, which comprises the following steps:
feeding the special feed for the fattening pigs in the preparation example 1;
the following breeding modes are adopted: feeding freely in 5 days, and feeding at a fixed time (1 time for each of 8:00, 14:00, and 20: 00) for 1 day, wherein the feeding amount is 75% of the free feeding amount.
The environment regulation mode is as follows: controlling the temperature in the pigsty at 23 ℃; the humidity is controlled to be 65%; CO 2 2 The concentration is controlled at 1700mg/m 3 ；NH 3 The concentration is controlled at 0.4mg/m 3 。
Example 2
The embodiment provides a breeding method for maintaining intestinal health of piglets, which comprises the following steps:
feeding the special feed for the fattening pigs in the preparation example 1;
the following breeding modes are adopted: feeding freely in 7 days, and feeding at a fixed time (1 time for each of 8:00, 14:00, and 20: 00) for 1 day, wherein the feeding amount is 70% of the free feeding amount.
The environment regulation mode is as follows: controlling the temperature in the pigsty at 24 ℃; controlling the humidity at 70%; CO 2 2 The concentration is controlled at 1500mg/m 3 ；NH 3 The concentration is controlled at 0.3mg/m 3 。
Examples 3 to 13
The present example provides a breeding method for maintaining intestinal health of piglets, which is different from example 1 only in that the special feed for piglets in preparation example 1 is replaced by the special feed for piglets in preparation examples 2 to 12, and other operations are kept unchanged.
Comparative example 1
The comparative example provides a piglet breeding method, which comprises the following steps:
feeding commercial fattening pig batch (proud biotechnology limited, Guangzhou);
the following breeding modes are adopted: feeding freely in 5 days, and feeding at a fixed time (1 time for each of 8:00, 14:00, and 20: 00) for 1 day, wherein the feeding amount is 75% of the free feeding amount.
The environment regulation mode is as follows: controlling the temperature in the pigsty at 23 ℃; the humidity is controlled to be 65%; CO 2 2 The concentration is controlled at 1700mg/m 3 ；NH 3 The concentration is controlled at 0.4mg/m 3 。
Comparative example 2
The comparative example provides a piglet breeding method, which comprises the following steps:
feeding the special feed for the fattening pigs in the preparation example 1;
adopting a free feeding mode;
the environment regulation and control mode is as follows: controlling the temperature in the pigsty at 23 ℃; the humidity is controlled to be 65%; CO 2 2 The concentration is controlled at 1700mg/m 3 ；NH 3 The concentration is controlled at 0.4mg/m 3 。
Comparative example 3
The comparative example provides a piglet breeding method, which comprises the following steps:
feeding the special feed for the fattening pigs in the preparation example 1;
the following breeding modes are adopted: feeding freely in 5 days, and feeding at a fixed time (1 time for each of 8:00, 14:00, and 20: 00) for 1 day, wherein the feeding amount is 75% of the free feeding amount.
The environment regulation mode is as follows: controlling the temperature in the pigsty at 28 ℃; controlling the humidity at 55%; CO 2 2 The concentration is controlled at 1700mg/m 3 ；NH 3 The concentration is controlled at 0.4mg/m 3 。
Comparative example 4
The comparative example provides a piglet breeding method, which comprises the following steps:
feeding the special feed for the fattening pigs in the preparation example 1;
the following breeding modes are adopted: feeding freely in 5 days, and feeding at a fixed time (1 time for each of 8:00, 14:00, and 20: 00) for 1 day, wherein the feeding amount is 75% of the free feeding amount.
The environment regulation and control mode is as follows: controlling the temperature in the pigsty at 23 ℃; the humidity is controlled to be 65%; is not to CO 2 Concentration and NH 3 The concentration is controlled.
Test example
170 healthy weaned-Du-growing three-way crossbred pigs with the weight of about 8kg are selected, randomly divided into 17 treatment groups, and cultured by adopting the culture modes of examples 1-13 and comparative examples 1-4 respectively, wherein the test period is 28 days.
(1) Growth performance was counted and the statistical results are shown in table 1 (presented as mean).
TABLE 1
(2) At the end of the test, 3 piglets with approximate average body weight are selected for slaughtering each group, the contents of the caecum and the colon are respectively collected in an aseptic operation mode, the cecum and the colon are evenly shaken in sterilized physiological saline, the quantity of intestinal lactobacilli and escherichia coli is counted by a flat culture counting method, and the quantity of flora is counted according to the quantity of each gram of intestinesLogarithm of the total number of bacterial colonies contained in the content of the tract (lgCFU/g)]And (4) showing. Weighing 1g of the content in a sterile operation table, shaking up in 99mL of sterilized normal saline with glass beads, putting 1mL of the content in a test tube, adding 9mL of sterile 0.9% normal saline, shaking and mixing uniformly, and sequentially diluting to 10 times in equal ratio according to 10 times -7 . Wherein the lactobacillus is inoculated on a lactobacillus selective medium (LBS) for culture, and is subjected to colony counting after anaerobic culture for 48 hours in a biochemical incubator at 37 ℃; escherichia coli was inoculated on eosin methylene blue agar medium and cultured in a 37 ℃ biochemical incubator for 24 hours, and then colony counting was performed. The statistical results are shown in table 2 (presented as mean).
TABLE 2
(3) After the test is finished, collecting blood of the anterior vena cava of each group of pigs, standing at 25 ℃, centrifuging at 1500 Xg for 10min, collecting serum, and freezing and storing at-80 ℃ to be tested. Serum cytokines were measured using a commercial pig-specific ELISA kit, and serum cytokine (IL-1. beta., IL-2, IL-6, and TNF-. alpha.) levels were measured following the protocol of the kit. The statistical results are shown in table 3 (presented as mean).
TABLE 3
As can be seen from the data in tables 1-3: compared with the feeding mode of a comparative example, the feeding mode can more effectively promote the metabolism of organisms, effectively kill or prefabricate pathogenic bacteria, clear free radicals in vivo, resist inflammation and oxidation, regulate intestinal flora and maintain intestinal health, thereby obviously reducing the feed ratio and improving the growth performance of piglets. And the fermentation compound, the fat compound, the feeding mode and the environmental factors all influence the technical effect.
The applicant states that the present invention is described by the above embodiments, but the present invention is not limited to the above embodiments, i.e. the present invention must not be implemented by the above embodiments. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.
It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.
Claims (10)
1. A breeding method for maintaining intestinal health of piglets is characterized by comprising the following steps:
feeding special feed for piglets: the special feed for the piglets comprises a base material, a fat compound, a fermentation compound and a premix;
feeding mode: intermittent feeding limitation;
and (3) environmental regulation: controlling temperature, humidity and CO in pigsty 2 Concentration and NH 3 And (4) concentration.
2. The breeding method for maintaining intestinal health of piglets according to claim 1, wherein the intermittent feeding limitation mode is as follows: 3-7 days, the feed intake is freely limited to 70-80% of the free feed intake in 1 day.
3. The cultivation method for maintaining the intestinal health of the piglets according to claim 1 or 2, wherein the temperature in the pigsty is controlled at 22-26 ℃ and the humidity is controlled at 60-70%;
preferably, the CO in the pig house 2 The concentration is controlled at 1500-2000mg/m 3 ，NH 3 The concentration is controlled to be 0.2-0.6mg/m 3 。
4. The breeding method for maintaining intestinal health of piglets according to any one of claims 1-3, wherein the fat complex comprises antarctic krill phospholipids, OMO structural lipids, inulin butyrate, coated plant essential oils, amino acids and carriers;
preferably, the fat complex comprises 10-20 parts of antarctic krill phospholipid, 20-40 parts of OMO structural lipid, 5-10 parts of inulin butyrate, 0.1-1 part of coated vegetable essential oil, 5-15 parts of amino acid and 30-60 parts of carrier in parts by weight based on the total weight of the fat complex;
preferably, the medium-chain fatty acid in the OMO structural lipid comprises caprylic acid, capric acid, lauric acid and myristic acid, and the mass ratio of the caprylic acid, the capric acid, the lauric acid and the myristic acid is (1-3): (1-5): (1-2): (3-5);
preferably, the coated plant essential oil comprises anise essential oil and capsicum essential oil;
preferably, the amino acids include leucine and valine;
preferably, the carrier comprises puffed corn flour and/or puffed corncob flour.
5. The breeding method for maintaining intestinal health of piglets according to claim 4, wherein the fat complex is prepared by a preparation method comprising the following steps:
heating and dissolving phospholipid and OMO structure lipid of Euphausia superba, mixing with inulin butyrate and amino acid, mixing with carrier, adsorbing, cooling, and mixing with coated plant essential oil;
preferably, the heating means heating to 60-100 ℃;
preferably, said cooling means cooling to 15-40 ℃.
6. The cultivation method for maintaining the intestinal health of piglets according to any one of claims 1 to 5, wherein the fermentation compound is a product obtained by microbial fermentation of a fermentation raw material;
the fermentation raw material comprises at least two of soybean meal, antarctic krill meal, moringa oleifera leaf meal, pueraria lobata powder, jerusalem artichoke powder and macleaya cordata leaf meal;
preferably, the fermentation raw material comprises a combination of soybean meal, antarctic krill meal, moringa oleifera leaf meal, pueraria lobata powder, jerusalem artichoke powder and macleaya cordata leaf meal;
preferably, the fermentation raw material comprises the following components in parts by weight based on the total weight of the fermentation raw material: 10-30 parts of soybean meal, 1-10 parts of antarctic krill powder, 30-50 parts of moringa oleifera leaf powder, 20-40 parts of radix puerariae powder, 5-10 parts of jerusalem artichoke powder and 10-30 parts of macleaya cordata leaf powder.
7. The method of claim 6, wherein the microorganisms comprise a combination of enterococcus faecalis, Bacillus subtilis, and Aspergillus oryzae;
preferably, the microorganism comprises 10-30 parts of enterococcus faecalis, 20-40 parts of bacillus subtilis and 20-40 parts of aspergillus oryzae in parts by weight based on the total weight of the microorganism;
preferably, the total weight of the microorganism is 5-10% of the total weight of the fermentation raw material;
preferably, the fermentation complex is prepared by the following method:
mixing the fermentation raw materials with bacillus subtilis and aspergillus oryzae for aerobic fermentation, then mixing with enterococcus faecalis for anaerobic fermentation, and drying to obtain the compound bacillus subtilis preparation;
preferably, the aerobic fermentation conditions are: the fermentation temperature is 25-40 ℃, the water content is 30-45%, and the fermentation time is 24-72 h;
preferably, the anaerobic fermentation conditions are: the fermentation temperature is 25-40 ℃, the water content is 30-45%, and the fermentation time is 48-96 h.
8. The method of claim any one of claims 1-7, wherein the substrate comprises corn;
preferably, the premix comprises a vitamin premix and a trace element premix.
9. The cultivation method for maintaining the intestinal health of the piglets according to any one of claims 1 to 8, wherein the preparation method of the special feed for the piglets comprises the following steps:
mixing the base material, the fat compound, the fermentation compound and the premix uniformly.
10. Use of a method according to any one of claims 1 to 9 for maintaining the intestinal health of piglets for improving the breeding efficiency of piglets.
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| JPH11289994A (en) * | 1998-04-09 | 1999-10-26 | Hideaki Tanaka | Formula feed containing additive having balanced nutrition |
| BR0104925A (en) * | 2001-10-26 | 2003-08-26 | Gilson Cesar Vituri | Animal food |
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