CN108813123A - It is a kind of improve intestine of young pigs health enteral nutrition packet and application - Google Patents

It is a kind of improve intestine of young pigs health enteral nutrition packet and application Download PDF

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CN108813123A
CN108813123A CN201810540190.4A CN201810540190A CN108813123A CN 108813123 A CN108813123 A CN 108813123A CN 201810540190 A CN201810540190 A CN 201810540190A CN 108813123 A CN108813123 A CN 108813123A
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weight
parts
enteral nutrition
acid
nutrition packet
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赵莹
黄红梅
张擎
汤飞飞
林丛梅
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SHANGHAI XINBANG BIOTECHNOLOGY Co Ltd
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SHANGHAI XINBANG BIOTECHNOLOGY Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes

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Abstract

The present invention relates to a kind of enteral nutrition packet for improving intestine of young pigs health and applications.The enteral nutrition packet, by weight, including following components：Complex enzyme formulation 2-4 parts by weight are coated with compound acidulant 20-30 parts by weight, compound probiotic 5-20 parts by weight, antibacterial peptide 5-20 parts by weight, plants essential oil 4-10 parts by weight, glutamine dipeptide 10-30 parts by weight, bile acid 4-8 parts by weight.The present invention can be improved pigling immunity, improve survival rate, daily gain and the feed conversion rate of weanling pig and child care piglet.

Description

It is a kind of improve intestine of young pigs health enteral nutrition packet and application
Technical field
The invention belongs to field of feed, and in particular to it is a kind of improve intestine of young pigs health enteral nutrition packet and answer With.
Background technique
The use of antibiotic and high zinc has apparent effect for piglet anti diar rhea, but since feeding antibiotic may The generation for inducing resistant bacterial strains, minerals caused by high-caliber inorganic zinc the reality such as are accumulated in the environment and are asked in daily ration Topic.To solve these problems, imperative is to find effective and feasible method to solve the problems, such as grice diarrhoea, it has often been said that pig raising It is exactly to support enteron aisle, therefore the health of intestine of young pigs is most important for the production of entire pig industry.
Enteron aisle is both the main place of animal digestion food absorption nutrition, and maximum immune organ in vivo.Piglet Enteron aisle has been formed in embryonic period, embryonic phase, but structure and function are all not perfect, and with the growth and development of piglet, its digestive function is gradually It is mature.
Weaning stress, childbearing period of piglets turn group reload stress, it is shown the characteristics of be feeding decline, intestinal disorder diarrhea Decline with growth performance, the death rate is high, this problem for being always puzzlement pig breeding industry and animal physiological nutrition educational circles are urgently One of proposition of solution.Wean leads to the significant changes of gastrointestinal physiology, microbial flora and immunity；Childbearing period of piglets turns group It reloads, destroys the script intestinal tissue that do not reach maturity and the active immunity not yet completely set up further And damage, and then growth performance continues to decline, or even dead.Therefore the present invention propose it is a kind of raising intestine of young pigs health it is compound Nutritional programs promote intestine of young pigs health, and then help the foundation as early as possible of its active immunity.
The patent of invention of 106666138 A of Publication No. CN specifically discloses a kind of nonreactive of promotion intestine of young pigs development Creep feed and preparation method thereof, creep feed include following components and parts by weight content：10-30 parts of corn, popcorn 10-30 Part, 10-18 parts of low albumen whey powder, 3-5 parts of fish meal, 7-12 parts of fermented bean dregs, 2-3 parts of soya-bean oil, 3-5 parts of sucrose, enzyme preparation 0.1-0.2 parts, 0.03-0.05 parts of flavouring agent, 0.05-0.1 parts of plants essential oil, 0.1-0.3 parts of compound probiotic, antibacterial peptide 0.05-0.1 parts, 0.05-0.08 parts of glutamine dipeptide, 0.04-0.06 parts of arginine and 2-5 parts of premix.The creep feed Intestinal regeneration and the development of piglet can be effectively facilitated, the digestion power and barrier ability of piglet itself is improved, diarrhea is effectively reduced Rate and disease incidence, but the space being still also improved.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide a kind of raising intestine of young pigs health Enteral nutrition packet, improve pigling immunity, improve survival rate, daily gain and the food conversion of weanling pig and child care piglet Rate.
The present invention also provides the application of the enteral nutrition packet of above-mentioned raising intestine of young pigs health simultaneously.
In order to solve the above technical problems, a kind of technical solution that the present invention takes is：
A kind of enteral nutrition packet improving intestine of young pigs health, by weight, including following components：Complex enzyme formulation 2- 4 parts by weight are coated with compound acidulant 20-30 parts by weight, compound probiotic 5-20 parts by weight, antibacterial peptide 5-20 parts by weight, plant Essential oil 4-10 parts by weight, glutamine dipeptide 10-30 parts by weight, bile acid 4-8 parts by weight.
Preferably, complex enzyme formulation includes zytase, protease, amylase, dextranase, mannonase pectin Enzyme, each component are mixed by carrier of glucose.
It is further preferred that enzymatic activity >=10000U/g of zytase, enzymatic activity >=10000U/g of protease, starch Enzymatic activity >=1000U/g of enzyme, enzymatic activity >=1000U/g of dextranase, enzymatic activity >=2500U/g of mannase, fruit Enzymatic activity >=250U/g of glue enzyme.
Complex enzyme formulation can make up for it the deficiency of piglet endogenous enzymes, to improve piglet to the digestibility and utilization of starch and protein Rate inhibits the effect of certain anti-nutritional factors, improves nutritive digestibility, improves piglet growth performance, while mannosan Enzyme, action pH range is wide, and decomposition efficiency is high, and it is good immunopotentiating agent that decomposition substrate, which is manna oligosacchride,.
Preferably, coating compound acidulant includes lactic acid, citric acid, fumaric acid, benzoic acid and phosphoric acid, have stablize, The features such as non-hygroscopic and good fluidity and Combination.
It is further preferred that the mass percent of lactic acid is 5-10%, the mass percent of citric acid is 8-10%, rich horse The mass percent of acid is 4-8%, and the mass percent of benzoic acid is 15-25%, and the mass percent of phosphoric acid is 5-10%, Remaining is carrier silicas.
The digestion of weanling pig and the piglet in small guarantor's stage and immune system also incomplete development are mature, and gastric acid secretion is not Foot is extremely difficult to PH in stomach can be made to reduce with comparable acidity level (2.0-3.5) in adult pig stomach, addition compound acidulant, Propepsin is activated, and further stimulates the secretion of trypsase, promotes the digestion and absorption of protein, and piglet digestive The interior development for keeping suitable acidity that can stimulate its digestive organs, can increase Small Intestine of Piglets relative length and relative weight.
Coating compound acidulant directly participates in energy i (in vivo) metabolism, as energy source：Lactic acid is the final production of glycolysis One of object can be released energy by gluconeogenesis as energy source, and fumaric acid, that is, fumaric acid can directly participate in three Carboxylic acid recycle energy supply.It is coated with compound acidulant and improves immunity of organisms and anti-stress effect：When body is under stress situation Lactic acid and fumaric acid can promptly synthesize ATP, guarantee the eubolism of body, improve livestock and poultry resistivity.In addition, young Acidulant is added in pannage can enhance the immune function of piglet, especially greatly enhance the immune function of red blood cell.
Preferably, effective viable bacteria content >=1 × 10 of compound probiotic10Cfu/g comprising lactic acid bacteria, bacillus subtilis Bacterium and saccharomycete, wherein viable count of lactobacillus >=1 × 109Cfu/g, bacillus subtilis viable count >=1 × 109Cfu/g, yeast Bacterium viable count >=1 × 109cfu/g。
Lactic acid bacteria is obligate anaerobe, has adhesiveness and colonization ability, is that (it is place that colonization resistance refers to composition colonization resistance The main resistance or resistance that pathogenic bacteria and potentially pathogenic organism are colonized and are bred in normal microflora) main power, The referred to as colonization resistance factor.Lactic acid bacteria is combined closely by adhesin and intestinal mucosa cells, is colonized occupy-place in intestinal mucosal surface, It is the chief component of physiology barrier.
Bacillus is aerobic bacteria, and there is biology to take oxygen ability by force, need to consume a large amount of oxygen during the growth process.Withered grass bud After spore bacillus enters animal intestinal tract, a large amount of free oxygen is consumed, enteral oxygen concentration and redox potential is reduced, improves cream The growing environment of the anaerobic bacterias such as acidfast bacilli, Bifidobacterium is conducive to the growth of anaerobic bacteria, keeps the steady of intestinal microecology system Weighing apparatus, while making the growth of aerobic bacteria enterobacteria etc. existing for script in enteron aisle because anoxic is suppressed, it is disease-resistant to improve animal body Ability reduces enterogastric diseases occurrence probability.
Saccharomycete is facultative anaerobic bacteria, enhance in intestine of young pigs brush border Disacchridase Activities, disease-resistant former adhesiving effect, with The toxin that pathogen generates competitively combines the binding site in enteron aisle, to reduce organism infection.
Compound probiotic promotes animal intestinal tract associated lymphoid tissue to be in " the immune preparation state " of height, and activation intestines are viscous Associated lymphoid tissue in film enhances SIgA antibody-secreting, improves Immune discrimination power, and induce T, bone-marrow-derived lymphocyte and macrophage Cell cytokine production activates systemic immune system by lymphocyte recirculation, to enhance the non-specificity of body And specific immune function, while can also increase the weight and size of immune organ (liver, spleen, lung).In addition, saccharomycete can also promote Into non-specific immune function, it is obviously promoted the generation of IgA and S-IgA.
Preferably, antibacterial peptide is silkworm antibacterial peptide, is one kind of host innate immune defense system generation with a variety of The micromolecule polypeptide of biological activity, silkworm antibacterial peptide potency >=160000U/g, bacillus subtilis >=1.0 × 109cfu/ g.Silkworm antibacterial peptide belongs to the strongest cecropin-B class antibacterial peptide of sterilizing power, is made of 37 amino acid residues, containing 25% α- Spiral amino acid, the amino acid of 32% stretched out structure and 43% random coil amino acid make day after gene modification Positive charge quantity in right silkworm antibacterial peptide greatly increases, and enhances its antibacterial activity, has broad spectrum antibacterial, thermal stability By force, good water solubility, be not likely to produce the features such as drug resistance, noresidue.
Preferably, plants essential oil includes following components and weight percent content：Cinnamic acid 15-25%, Thymol 3- 5%, carvacrol 3-5%, eugenol 3-5%, remaining is carrier, and carrier selects silica, gelatin, converted starch, stearic acid One of or it is a variety of, utilize closed cycle freezing granulator granulation.
Plants essential oil is the natural volatile oil with aromatic odor being extracted from plants, and there is strong wide spectrum to kill Bacterium property, the loss of feed nutrient can be slowed down by being added in feed, and stimulation poultry is fed, and reduce the micro- life of nocuousness of gastrointestinal tract Object improves intestinal beneficial microbes, improves internal digestive enzyme activity, enhance the absorption of nutriment, to improve disappearing for feed Change utilization rate and poultry speed of weight increment, reduces feedstuff-meat ratio and feeding cost.
Plants essential oil proportion of the present invention rationally, has good bactericidal ability, while having good internal antioxygen Change ability；Using gelatin, the materials such as converted starch are pelletized using closed cycle freezing granulator, are handled by special coating, Effective component can uniformly discharge at each position of animal body, it is ensured that effective component is able to enter the middle end of enteron aisle；By special Coating processing, effective component can discharge completely in animal body；Handled by special coating, effective component in storage and Storage rate is up to 95% or more in pelletization.
Glutamine dipeptide of the present invention is alanyl-glutamine, is purchased from Shanghai Chaoqiang Chemical Co., Ltd., (N- (2)- L-alanyl-L-glutamine, Ala-Gln) its molecular formula be C8H15N3O4, molecular weight 217.22, purity >=99%.
Glutamine dipeptide can hydrolyze after entering animal body by small intestinal mucosa absorbed intact, and by aminopeptidase, release Gln improves blood plasma Gln concentration, to play the special physiological function of Gln.Glutamine dipeptide is rapidly decomposed into vivo Ala and Gln principally serves as the nitrogen source of body and the energy or is utilized synthetic proteins matter.It is interrupted since Disease in Infants Gln absorbs, In addition the exogenous Gln absorptivity decline of bring, the intracorporal Gln of piglet almost exhaust due to intestinal mucosa injury, intestinal immunity Decline therewith with resistance to oxidation, intestinal mucosa injury is caused further to deteriorate.At this point, Gln, which is replenished in time, can promote piglet enteric epithelium Cell repair mitigates intestinal mucosa injury, prevents Villus atrophy.
Glutamine dipeptide provides oxygenated fuel and nitrogen source by release Gln for small intestinal mucosa.Under normal physiological condition, Gln needed for enteron aisle is derived from and is synthesized and ingest in vivo, and in stress situation, body will greatly increase the consumption of Gln, body Interior synthesis is far from meeting body needs.When the endogenous Gln from blood plasma and muscle in piglet enteric epithelium is not enough in maintenance Chrotoplast it is complete when, significant change will occur for intestinal tissue structure, show as Villus atrophy, crypts hyperplasia so that influence it is small Absorption of the intestines to nutrient.Therefore, differentiation, the increasing of Intestinal epithelial cells will be promoted to weanling pig supplement glutamine dipeptide It grows, and then protects the complete of intestinal mucosa structure and function.Glutamine dipeptide passes through up-regulation enterocyte tight junction protein The expression of Occludin and the permeability for reducing enteric epithelium.
Bile acid of the present invention is provided by Shandong Long Chang animal health-care product Co., Ltd, trade name cream energy, main component packet Include bile acid (30%).Addition bile acid can solve swinery itself secretion endogenous emulsifier it is insufficient caused by energy dissipation and because Nutritional imbalance bring health problem, reduces the retardance of piglet fat digestion, and energy dissipation induces liver and gallbladder metabolic disorder and disease The risk of disease.For high fat diet, disappearing for its energy is can be improved in the addition of the bile acid suckling piglet incomplete for hepato-biliary function Change ability, and the recovery and raising of its hepato-biliary function are dynamically assisted, promote body health, reaches the mesh for rationally utilizing energy 's.
Present invention simultaneously provides a kind of enteral nutrition packets of above-mentioned raising intestine of young pigs health in weanling pig daily ration Application, the mass ratio of enteral nutrition packet and Earlier-stage compound feed for piglets is 1-2:100, preferably 1:100.
Present invention simultaneously provides a kind of enteral nutrition packets of above-mentioned raising intestine of young pigs health in childbearing period of piglets daily ration In application, enteral nutrition packet and piglet concentration daily ration mass ratio be 1-3:100, preferably 2-3:100.
Due to the use of above technical scheme, the present invention has the following advantages that compared with prior art：
The present invention is coated with the application of compound acidulant with piglet gastric acid secretion after making up wean not by complex enzyme formulation Foot, digestive ferment hyposecretion solve the physiological defect of particular time；By compound probiotic, antibacterial peptide, plants essential oil improves intestines Microbial flora balance in road improves the problems such as Small Intestinal is weak, and solution immunity is poor；Pass through glutamine dipeptide, bile The use of acid reduces the damage of enteron aisle, accelerates the reparation of enteron aisle, and the final present invention improves piglet by improving intestine of young pigs health Immunity improves survival rate, daily gain and the feed conversion rate of weanling pig and child care piglet.
Detailed description of the invention
Fig. 1 is 1 test group intestinal villi developmental state figure of the embodiment of the present invention；
Fig. 2 is 1 control group intestinal villi developmental state figure of the embodiment of the present invention.
Specific embodiment
The invention will be further described with reference to the accompanying drawings and embodiments.It should be understood that these embodiments are only used for The bright present invention, rather than limit the scope of protection of the present invention, what technical staff made according to the present invention in practical applications changes Into and adjustment, still fall within protection scope of the present invention.
Embodiment 1
A kind of enteral nutrition packet for improving intestine of young pigs health of the present embodiment, by weight, including following components：It is compound 4 parts by weight of enzyme preparation are coated with 30 parts by weight of compound acidulant, 20 parts by weight of compound probiotic, 20 parts by weight of antibacterial peptide, plant essence Oily 10 parts by weight, 30 parts by weight of glutamine dipeptide, 8 parts by weight of bile acid.
Complex enzyme formulation includes zytase, protease, amylase, dextranase, mannonase pectase.
Enzymatic activity >=10000U/g of zytase, enzymatic activity >=10000U/g of protease, the enzymatic activity of amylase >= 1000U/g, enzymatic activity >=1000U/g of dextranase, enzymatic activity >=2500U/g of mannase, the enzymatic activity of pectase ≥250U/g。
The mass percent for being coated with lactic acid in compound acidulant is 7%, and the mass percent of citric acid is 10%, fumaric acid Mass percent be 6%, the mass percent of benzoic acid is 20%, and the mass percent of phosphoric acid is 5%, remaining is carrier two Silica.
Effective viable bacteria content >=1 × 10 of compound probiotic10Cfu/g comprising lactic acid bacteria, bacillus subtilis and yeast Bacterium, wherein viable count of lactobacillus >=1 × 109Cfu/g, bacillus subtilis viable count >=1 × 109Cfu/g, it is described Saccharomycete viable count >=1 × 109cfu/g。
Antibacterial peptide be silkworm antibacterial peptide, silkworm antibacterial peptide potency >=160000U/g, bacillus subtilis >=1.0 × 109cfu/g。
Plants essential oil includes following components and weight percent content：Cinnamic acid 20%, Thymol 4%, carvacrol 5%, eugenol 3%, remaining is carrier, and carrier selects gelatin, and converted starch is pelletized using closed cycle freezing granulator.
Glutamine dipeptide is alanyl-glutamine, is purchased from Shanghai Chaoqiang Chemical Co., Ltd..
Bile acid is provided by Shandong Long Chang animal health-care product Co., Ltd, trade name cream energy.
The present embodiment improves application of the enteral nutrition packet of intestine of young pigs health in weanling pig daily ration, the enteron aisle battalion The mass ratio for supporting packet and Earlier-stage compound feed for piglets is 1:100.
Efficacy assessments test
To verify the present embodiment effect, test specific implementation is as follows：
This test carries out on Jiangsu Taizhou Red Star pig farm, and the piglet early-stage that control group piglet feeds market routine, which cooperates, raises Material, the identical market routine Earlier-stage compound feed for piglets of test group fed control group, and match by mass fraction 1% in piglet early-stage Close the enteral nutrition packet that the present embodiment is added in feed.
Feeding management：Feeding, water supply, immune etc. are carried out according to the normal production procedure in pig farm.
1. measuring based growth index：
23 age in days of piglet is recorded to wean to the whole story weight of 35 ages in days religion trough end, daily ingestion amount, daily gain, diarrhea rate, The indexs such as feedstuff-meat ratio.
Diarrhea rate (%)=Σ (diarrhea piglet head number × Diarrhoea days)/(the total head number × overall test number of days of piglet) × 100
2. measuring protective effect of the enteron aisle protection packet to intestinal mucosa
When 35 age in days, piglet each 10 for killing test group and control group are cutd open at random, Bacillus acidi lactici contains in measurement intestines mucus The content of IgA in amount and mucus, and observe the developmental state of intestinal villi.
(1) in mucus Bacillus acidi lactici content detection
Its duodenum, jejunum, ileum, caecum and each 1cm in colon middle section or so are taken respectively, are longitudinally splitted, are gently removed Content, is rinsed 3 times with the PBS solution of sterile 0.01mol/L, is scraped surface mucus with sterile slide, is collected in EP pipe, With physiological saline doubling dilution, the content of Bacillus acidi lactici in each group lotion is detected using viable plate counts.
(2) IgA slightly proposes the detection of content in mucus
It takes test group and control group to cut open the Small Intestine of Piglets mucus killed, is separately added into the PBS of isometric 0.01mol/L, mix 3000r/min after even, centrifugation 10min take supernatant, detect wherein protein content with nucleic acid-protein instrument.
IgA's slightly mentions：1ml mucus is diluted to 10ml with PBS, and isometric saturated ammonium sulfate is added dropwise while stirring, mixes, Make the saturation degree 50% of saturated ammonium sulfate, 4 DEG C of standing 30min, then with 3000r/min, 30min is centrifuged, by precipitating physiology Salt water dilution, is further continued for that saturated ammonium sulfate is slowly added dropwise, and makes its saturation degree up to 40%, stands, and centrifugation is repeated 2 times.Precipitating life After managing salt water dilution, wherein protein content is detected with nucleic acid-protein instrument.
(3) developmental state of intestinal villus and Intestinal Mucosal Tissues
Jejunum 2cm or so is taken, is rinsed well with the PBS of 0.01mol/L, is fixed with 10% formalin, sample is through de- After water, paraffin embedding, slice and dyeing, the developmental state of enteron aisle villus and intestinal mucosa is observed under an optical microscope.
Test result
1, growth performance
2, Bacillus acidi lactici content (unit in intestines mucus：107cfu/g)
Duodenum Jejunum Ileum Caecum Colon
Control group 2.52 0.98 0.18 0.11 0.08
Test group 6.3 7.7 6.5 7.9 8.8
The duodenum of test group, jejunum, ileum, caecum and each intestinal segment of colon open-minded liquid in Bacillus acidi lactici content it is bright Aobvious to be higher than control group, bacterial content is more stable, maintains essentially in 107Cfu/g or so, and the fluctuation of the Bacillus acidi lactici of control group compared with Greatly, up to duodenum 2.52 × 107Cfu/g, and only the 0.08 × 10 of minimum colon7Cfu/g, increasingly with intestinal segment It is in reduction trend close to rectum.
Protein content is either all apparently higher than control group after slightly still slightly mentioning in advance in the mucus of test group.Slightly mention Before, protein content is 16.696mg/ml in the mucus of test group, and control group is 12.518mg/ml, higher than control group 25%.After slightly mentioning, protein content is 1.2052mg/ml in the mucus of test group, and control group is 0.786mg/ml, is compared It is high by 34.8% according to organizing.
In light microscopic observation after HE is dyed, for test group small intestinal mucosa epithelial development than more complete, villus is longer, arranges whole Neat fine and closely woven, brush border is high-visible, no congested and oedema, as shown in Figure 1.And control group epithelium has stove to fall off appearance, epithelium Slight thinning, villus is shorter, and arrangement is more random, and mucosa lamina propria is slightly congested, and oedema is in confused situation aobvious, as shown in Fig. 2.
By the test result of the present embodiment 1 it is found that test group is compared with control group, 12 days piglets day after test group wean Weight gain improves 17.7% than control group, and daily ingestion amount improves 3.3%, and feedstuff-meat ratio reduces by 10.5%, and diarrhea rate reduces by 65.9%, The death rate reduces by 3.43%, and measures enteral nutrition packet and play important function, analysis enteron aisle battalion to the protection of intestinal mucosa Feeding packet improves the quantity of Bacillus acidi lactici, it is suppressed that rapid breeding of the Escherichia coli in intestine of young pigs, to reduce large intestine Flora ratio of the bacillus in intestine of young pigs, eventually reduces diarrhea ratio, improves the survival rate of weanling pig.
Embodiment 2
A kind of enteral nutrition packet for improving intestine of young pigs health of the present embodiment, by weight, including following components：It is compound 4 parts by weight of enzyme preparation are coated with 30 parts by weight of compound acidulant, 15 parts by weight of compound probiotic, 15 parts by weight of antibacterial peptide, plant essence Oily 8 parts by weight, 30 parts by weight of glutamine dipeptide, 8 parts by weight of bile acid.
Complex enzyme formulation includes zytase, protease, amylase, dextranase, mannonase pectase.
Enzymatic activity >=10000U/g of zytase, enzymatic activity >=10000U/g of protease, the enzymatic activity of amylase >= 1000U/g, enzymatic activity >=1000U/g of dextranase, enzymatic activity >=2500U/g of mannase, the enzymatic activity of pectase ≥250U/g。
The mass percent for being coated with lactic acid in compound acidulant is 8%, and the mass percent of citric acid is 9%, fumaric acid Mass percent be 7%, the mass percent of benzoic acid is 21%, and the mass percent of phosphoric acid is 5%, remaining is carrier two Silica.
Effective viable bacteria content >=1 × 10 of compound probiotic10Cfu/g comprising lactic acid bacteria, bacillus subtilis and yeast Bacterium, wherein viable count of lactobacillus >=1 × 109Cfu/g, bacillus subtilis viable count >=1 × 109Cfu/g, it is described Saccharomycete viable count >=1 × 109cfu/g。
Antibacterial peptide be silkworm antibacterial peptide, silkworm antibacterial peptide potency >=160000U/g, bacillus subtilis >=1.0 × 109cfu/g。
Plants essential oil includes following components and weight percent content：Cinnamic acid 19%, Thymol 5%, carvacrol 4%, eugenol 4%, remaining is carrier, and carrier selects silica, gelatin, converted starch, and stearic acid is cold using closed cycle Freeze granulator granulation.
Glutamine dipeptide is alanyl-glutamine, is purchased from Shanghai Chaoqiang Chemical Co., Ltd..
Bile acid is provided by Shandong Long Chang animal health-care product Co., Ltd, trade name cream energy.
The present embodiment improves application of the enteral nutrition packet of intestine of young pigs health in childbearing period of piglets daily ration, enteral nutrition The mass ratio of packet and piglet concentration daily ration is 2:100 (adding proportion is mass fraction 0.5% in batch).
Efficacy assessments test
To verify the present embodiment effect, test specific implementation is as follows：
This test carries out on Jiangsu Taizhou Red Star pig farm, and control group piglet feeds the concentrated feed for piglet of market routine, examination The identical market routine concentrated feed for piglet of group fed control group is tested, and adds the enteron aisle battalion of the present embodiment by mass fraction 2% Packet is supported, and is configured to batch by product requirement and uses.
Feeding management：Feeding, water supply, immune etc. are carried out according to the normal production procedure in pig farm.
1, based growth index is measured：
The whole story weight that record 35 age in days of piglet wean terminates to the small child care of 55 ages in days, daily ingestion amount, daily gain, diarrhea Rate, the indexs such as feedstuff-meat ratio.
Diarrhea rate (%)=Σ (diarrhea piglet head number × Diarrhoea days)/(the total head number × overall test number of days of piglet) × 100
Test result
1, growth performance
By the test result of the present embodiment 2 it is found that test group is compared with control group, the piglet of 20 days small child care phases of test group Daily gain improves 11.6% than control group, and daily ingestion amount improves 3.65%, and feedstuff-meat ratio reduces by 9.15%, and diarrhea rate reduces 63.6%, the death rate reduces by 2.53%, because the small child care stage, still not up to adult was horizontal for intestinal growth at this time, and active immunity It does not completely set up, therefore this stage builds using enteral nutrition packet to intestine of young pigs enteron aisle and the quick of active immunity is established all Play the role of positive.
Embodiment 3
A kind of enteral nutrition packet for improving intestine of young pigs health of the present embodiment, by weight, including following components：It is compound 4 parts by weight of enzyme preparation, 30 parts by weight of compound acidulant, 10 parts by weight of compound probiotic, 10 parts by weight of antibacterial peptide, plants essential oil 8 Parts by weight, 10 parts by weight of glutamine, 4 parts by weight of bile acid.
Complex enzyme formulation includes zytase, protease, amylase, dextranase, mannonase pectase.
Enzymatic activity >=10000U/g of zytase, enzymatic activity >=10000U/g of protease, the enzymatic activity of amylase >= 1000U/g, enzymatic activity >=1000U/g of dextranase, enzymatic activity >=2500U/g of mannase, the enzymatic activity of pectase ≥250U/g。
The mass percent for being coated with lactic acid in compound acidulant is 7%, and the mass percent of citric acid is 10%, fumaric acid Mass percent be 6%, the mass percent of benzoic acid is 19%, and the mass percent of phosphoric acid is 9%, remaining is carrier two Silica.
Effective viable bacteria content >=1 × 10 of compound probiotic10Cfu/g comprising lactic acid bacteria, bacillus subtilis and yeast Bacterium, wherein viable count of lactobacillus >=1 × 109Cfu/g, bacillus subtilis viable count >=1 × 109Cfu/g, it is described Saccharomycete viable count >=1 × 109cfu/g。
Antibacterial peptide be silkworm antibacterial peptide, silkworm antibacterial peptide potency >=160000U/g, bacillus subtilis >=1.0 × 109cfu/g。
Plants essential oil includes following components and weight percent content：Cinnamic acid 17%, Thymol 5%, carvacrol 5%, eugenol 5%, remaining is carrier, selects and uses gelatin, converted starch, is pelletized using closed cycle freezing granulator.
Glutamine dipeptide is alanyl-glutamine, is purchased from Shanghai Chaoqiang Chemical Co., Ltd..
Bile acid is provided by Shandong Long Chang animal health-care product Co., Ltd, trade name cream energy.
The present embodiment improves application of the enteral nutrition packet of intestine of young pigs health in childbearing period of piglets daily ration, enteral nutrition The mass ratio of packet and piglet concentration daily ration is 3:100 (adding proportion is mass fraction 0.36% in batch).
Efficacy assessments test
To verify the present embodiment effect, test specific implementation is as follows：
This test carries out on Jiangsu Taizhou Red Star pig farm, and control group piglet feeds the concentrated feed for piglet of market routine, examination The identical market routine concentrated feed for piglet of group fed control group is tested, and adds the enteron aisle battalion of the present embodiment by mass fraction 3% Packet is supported, and is configured to batch by product requirement and uses.
Feeding management：Feeding, water supply, immune etc. are carried out according to the normal production procedure in pig farm.
1, based growth index is measured：
The whole story weight that record 55 age in days of piglet wean terminates to the small child care of 75 ages in days, daily ingestion amount, daily gain, diarrhea Rate, the indexs such as feedstuff-meat ratio.
Diarrhea rate (%)=Σ (diarrhea piglet head number × Diarrhoea days)/(the total head number × overall test number of days of piglet) × 100
Test result
1, growth performance
By the test result of the present embodiment 3 it is found that test group is compared with control group, the piglet of 20 days big child care phases of test group Daily gain improves 11.6% than control group, and daily ingestion amount improves 3.65%, and feedstuff-meat ratio reduces by 5.26%, and diarrhea rate reduces 18%, the death rate reduces by 1.53%, applies enteral nutrition packet still to play the role of the growth of piglet in this stage positive.
Comparative example
Comparative example 1
This comparative example provides documents CN106666138A, i.e., a kind of promotion intestine of young pigs hair invented before our company The nonreactive creep feed educated, including following components and parts by weight content：25 parts of corn, 18 parts of popcorn, low albumen whey powder 12 Part, 4 parts of fish meal, 10 parts of fermented bean dregs, 2 parts of soya-bean oil, 4 parts of sucrose, 0.1 part of enzyme preparation, 0.04 part of flavouring agent, plants essential oil 0.08 Part, it is 0.2 part of compound probiotic, 0.08 part of antibacterial peptide, 0.06 part of glutamine dipeptide, 3 parts of 0.05 part of arginine and premix, swollen Change 12 parts of soybean, 4 parts of glucose, 0.4 part of acidulant and 0.2 part of zinc oxide.
Acidulant is compounded by organic acid with inorganic acid, wherein organic acid is lactic acid, and inorganic acid is orthophosphoric acid.Enzyme system Agent is zytase.Flavouring agent is purchased from finished product in the happy milk flavoured type flavouring agent for reaching (Guangzhou) flavouring agent Co., Ltd.
Compound probiotic is made of lactic acid bacteria, bacillus subtilis and saccharomycete, wherein and viable count of lactobacillus >=>=1 × 109Cfu/g, bacillus subtilis viable count >=1 × 109Cfu/g, saccharomycete viable count >=1 × 109cfu/g。
Antibacterial peptide is potency >=40000 units/g and bacillus subtilis bacterium number >=1 × 109The silkworm antibacterial peptide of cfu/g.
Premix is selected from the new nation K4051 premix in commercially available Shanghai.
Comparative example 2
Commercially available routine groove feed for pigling.
Experimental example
Further to verify effect of the present invention, implement following test：
This test carries out on Jiangsu Taizhou Red Star pig farm, test piglet is divided into 3 groups, and A, B component Si Wei not comparative examples 1,2 Feed, C group feed the market routine groove feed for pigling in comparative example 2, and the enteron aisle battalion of embodiment 1 is added by mass fraction 1% Support packet.Feeding management：Feeding, water supply, immune etc. are carried out according to the normal production procedure in pig farm.
1, based growth index is measured：
Record whole story weight of 23 age in days of the piglet wean to 50 ages in days, daily ingestion amount, daily gain, diarrhea rate, feedstuff-meat ratio etc. Index.
Diarrhea rate (%)=Σ (diarrhea piglet head number × Diarrhoea days)/(the total head number × overall test number of days of piglet) × 100
Test result
1, growth performance
Project A B C
Piglet number 351 321 342
It originates in age in days (day) 23 23 23
Initial weight kg 6.7 6.6 6.6
Terminate age in days 50 50 50
End weight kg 17.8 16.1 18.1
Daily ingestion amount g 603 587 588
Daily gain g 411 352 426
Feedstuff-meat ratio 1.47 1.67 1.38
Diarrhea rate 0.9% 3.8% 0.2%
Survival rate 98.43% 98.75% 99.05%
By experimental example test result it is found that comparative example 1 can actually improve piglet compared to commercially available conventional groove feed for pigling Growth performance reduces material ratio, reduces grice diarrhoea rate.But commercially available conventional groove feed for pigling adds enteron aisle battalion of the present invention After supporting packet, piglet growth performance can be significantly improved, diarrhea is reduced, reduces material ratio, have the effect of better than comparative example 1, and The stringent optimization carried out on the composition of intestinal health protection packet and ratio, is not only limited to the creep feed stage, may extend into In the small child care stage, effect is substantially better than the test effect of comparative example 1 in test in jumpbogroup, applies enteral nutrition packet in this stage Play the role of to the healthy growth of piglet positive.
The present invention relates to a kind of enteral nutrition packets for improving intestine of young pigs health, by adding complex enzyme system in daily ration Agent is coated with the application of compound acidulant to make up piglet gastric acid secretion deficiency after wean, digestive ferment hyposecretion, when solving special The ability that enteron aisle digests and assimilates nutriment can be improved in the physiological defect of phase, supports function of intestinal canal；And compound probiotic can be with The disorder of intestinal flora is adjusted, stimulation of the enterotoxin of such as Escherichia coli secretion to enterocyte is reduced, to protect The growth and development of intestinal villi, are equally beneficial for the absorption of piglet nutrition, also promote and protect the development of villus, pass through Compound probiotic, antibacterial peptide, plants essential oil improve intestinal microflora balance, and improvement Small Intestinal is weak, and immunity is poor The problems such as；By glutamine dipeptide, the use of bile acid compound nutritional packet reduces the damage of enteron aisle, accelerates the reparation of enteron aisle. Finally of the invention pass through improves intestine of young pigs health, raising pigling immunity, the survival rate of raising weanling pig and child care piglet, Daily gain and feed conversion rate.
Under the teaching of the present invention and the above embodiments, those skilled in the art are easy to it is envisioned that cited by the present invention Or each raw material enumerated or its equivalent alterations, each processing method or its equivalent alterations can realize the present invention and each original The parameter bound value of material and processing method, interval value can realize the present invention, embodiment numerous to list herein.
The present invention is described in detail above, the explanation of embodiment be merely used to help understand method of the invention and Its core concept, its object is to allow the personage for being familiar with this field technology to can understand the content of the present invention and implement it accordingly, and The protection scope that the present invention cannot be limited in this way.Any equivalent change or modification in accordance with the spirit of the invention should all be contained Lid is within protection scope of the present invention.

Claims (10)

1. a kind of enteral nutrition packet for improving intestine of young pigs health, it is characterised in that：By weight, including following components：It is multiple Synthase preparation 2-4 parts by weight are coated with compound acidulant 20-30 parts by weight, compound probiotic 5-20 parts by weight, antibacterial peptide 5-20 weight Measure part, plants essential oil 4-10 parts by weight, glutamine dipeptide 10-30 parts by weight, bile acid 4-8 parts by weight.
2. enteral nutrition packet according to claim 1, it is characterised in that：The complex enzyme formulation includes zytase, egg White enzyme, amylase, dextranase, mannonase pectase.
3. enteral nutrition packet according to claim 2, it is characterised in that：The enzymatic activity of the zytase >=10000 U/ G, enzymatic activity >=10000 U/g of the protease, enzymatic activity >=1000 U/g of the amylase, the enzyme of the dextranase Activity >=1000 U/g, enzymatic activity >=2500 U/g of the mannase, enzymatic activity >=250 U/g of the pectase.
4. enteral nutrition packet according to claim 1, it is characterised in that：The coating compound acidulant includes lactic acid, lemon Lemon acid, fumaric acid, benzoic acid and phosphoric acid.
5. enteral nutrition packet according to claim 4, it is characterised in that：The mass percent of the lactic acid is 5-10%, institute The mass percent for stating citric acid is 8-10%, and the mass percent of the fumaric acid is 4-8%, the quality percentage of the benzoic acid Than for 15-25%, the mass percent of the phosphoric acid is 5-10%, remaining is silica.
6. enteral nutrition packet according to claim 1, it is characterised in that：Effective viable bacteria content of the compound probiotic >= 1×1010Cfu/g, wherein viable count of lactobacillus >=1 × 109Cfu/g, bacillus subtilis viable count >=1 × 109Cfu/g, saccharomycete viable count >=1 × 109 cfu/g。
7. enteral nutrition packet according to claim 1, it is characterised in that：The antibacterial peptide is silkworm antibacterial peptide, silkworm Antibacterial peptide potency >=160000U/g, bacillus subtilis >=1.0 × 109 cfu/g。
8. enteral nutrition packet according to claim 1, it is characterised in that：The plants essential oil includes following components and weight Degree：Cinnamic acid 15-25%, Thymol 3-5%, carvacrol 3-5%, eugenol 3-5%, remaining is carrier, carrier choosing With silica, gelatin, converted starch, one of stearic acid or a variety of.
9. a kind of if the enteral nutrition packet described in any item of the claim 1 to 8 for improving intestine of young pigs health is in weanling pig The mass ratio of application in daily ration, the enteral nutrition packet and Earlier-stage compound feed for piglets is 1-2:100.
10. a kind of if the enteral nutrition packet described in any item of the claim 1 to 8 for improving intestine of young pigs health is in piglet cradling The mass ratio of application in phase daily ration, the enteral nutrition packet and piglet concentration daily ration is 1-3:100.
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CN109349446A (en) * 2018-12-14 2019-02-19 江苏奥迈生物科技有限公司 A kind of milking sow premix and preparation method thereof reducing breeding process ammonia nitrogen discharge amount
CN110074279A (en) * 2019-05-24 2019-08-02 四川品信饲料有限公司 A kind of child care compound feed prescription for promoting intestine of young pigs health reduction diarrhea rate
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CN114009608A (en) * 2021-11-05 2022-02-08 四川农业大学 Compound nutrient capable of repairing damaged intestinal tracts of piglets and application thereof
CN113995055A (en) * 2021-11-09 2022-02-01 四川省畜牧科学研究院 Piglet feed additive and preparation method thereof
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