CN109770045A - A kind of ecological, environmental protective pannage and its preparation method and application - Google Patents
A kind of ecological, environmental protective pannage and its preparation method and application Download PDFInfo
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- CN109770045A CN109770045A CN201910171929.3A CN201910171929A CN109770045A CN 109770045 A CN109770045 A CN 109770045A CN 201910171929 A CN201910171929 A CN 201910171929A CN 109770045 A CN109770045 A CN 109770045A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention discloses a kind of ecological, environmental protective pannage, including below according to the raw material for accounting for feed total weight percent: 20-30% rice chaff, 5-20% fermentation substrate, 0.0015-0.03% probiotics；Probiotics includes 0.0005-0.01% bacillus, 0.0005-0.01% saccharomycete and 0.0005-0.01% actinomyces.It can using ecological, environmental protective pannage provided by the invention feeding pig, be conducive to the strain and enzyme activity level of pig manure fermentation in pig manure by improving, by serving as the conditioner of pig manure fermentation in pig manure not to be entrained in by the rice chaff that pig digests, so that the pig manure being discharged out of pig body be enable to ferment automatically without additional addition microbial inoculum.
Description
Technical field
The invention belongs to livestock culturing field of fodder, and in particular, to a kind of ecological, environmental protective pannage and preparation method thereof
And application.
Background technique
The development of livestock and poultry breeding industry brought breeding pollution while improving people's living standard also increasingly causes people
Attention.Meanwhile containing nutritional ingredients such as nitrogen, phosphorus, potassium necessary to a large amount of crop growths in farm animal excrement and largely
Organic matter, pass it through compost fermentation curing after as organic fertilizer returning to the field be presently the most easily and economically processing utilization
Mode.
Currently, mainly promoting manure fermentation by directly adding additional microbial inoculum into feces of livestock and poultry.However, feces of livestock and poultry
As the excreta of animal, component and content are influenced by protein content in feed and probiotics.But according to existing skill
Art, influence of the feed fermentation to manure fermentation are not yet clear.Therefore, being made up of to reach setting feed keeps fowl and animal excrement automatic
The scheme of fermentation is rarely reported.
Summary of the invention
The object of the present invention is to provide a kind of ecological, environmental protective pannages and its preparation method and application, to solve above-mentioned technology
At least one of problem.
According to an aspect of the present invention, a kind of ecological, environmental protective pannage is provided, includes following according to accounting for feed gross weight
Measure the raw material of percentage: 20-30% rice chaff, 5-20% fermentation substrate, 0.015-0.18% probiotics；Probiotics includes 0.005-
0.06% bacillus, 0.005-0.06% saccharomycete and 0.005-0.06% actinomyces.
Preferably, probiotics further includes accounting for feed total weight percent 0.005-0.06% lactic acid bacteria.
Preferably, lactic acid bacteria is lactobacillus plantarum.
It preferably, further include accounting for feed total weight percent 0.0015-0.018% enzyme preparation, enzyme preparation is selected from cellulose
Enzyme, Niu Laisi enzyme and Jie Fu can one of enzymes or a variety of.
Preferably, enzyme preparation according to the group for accounting for enzyme preparation total weight percent by being grouped as below: 20-40% cellulose
Enzyme, 20-40% button Lay think enzyme and 20-40% outstanding person's richness can enzyme.
Preferably, fermentation substrate includes following according to the raw material for accounting for fermentation substrate total weight percent: 80-95% dregs of beans,
5-20% wheat-middlings.
Preferably, above-mentioned ecological, environmental protective pannage is prepared as follows: (1) by probiotics and the enzyme preparation 40
Activation is cured by oxygen therapy in DEG C water；(2) benefit is configured to according to material water volume ratio 1:0.5 addition water into liquid mixture obtained by step (1)
Raw bacterium solution, is then seeded into the fermentation substrate；(3) at 35 DEG C, solid anaerobic digestion；(4) the mixture hair of step (3)
After ferment is complete, rice chaff is added thereto.
Preferably, fermentation substrate includes following according to the raw material for accounting for fermentation substrate total weight percent: 55-70% dregs of beans,
20-30% cotton dregs, 1-5% mountain flour, 0.5-2% calcium monohydrogen phosphate, 0.5-2% sodium chloride and the more mines of 0.5-2% piggy.
Preferably, above-mentioned ecological, environmental protective pannage is prepared as follows: (1) probiotics and the enzyme preparation being existed
Activation is cured by oxygen therapy in 35-45 DEG C of water；(2) it is prepared into liquid mixture obtained by step (1) according to material water volume ratio 1:0.5 addition water
At prebiotic bacterium solution, it is then seeded into fermentation substrate；(3) at 35 DEG C, aerobic fermentation, at interval of 12 hours after fermentation 24 hours
Turning and throwing once；(4) after fermentation carries out 72 hours, the rice chaff is added into gained mixture.
According to another aspect of the present invention, a kind of method for making pig manure voluntarily ferment is provided, above-mentioned ecological, environmental protective is used
Pannage feeds pig.
Ecological, environmental protective pannage provided by the invention has the advantage that
1. the component using the rice chaff of certain dosage as pannage, can be under the premise of not influencing pig growth quality
Meet the satiety of pig, reduce feed cost, improve agricultural production waste product utilization rate, the rice chaff not digested by pig is with pig manure
It is discharged, the conditioner of pig manure fermentation can be served as by mixing the rice chaff in pig manure, promote pig manure spontaneous fermentation.
2. determining appropriate probiotic combinations by screening, it is allowed to directly raise with addition feed after fermentation substrate co-fermentation
Pig feeding only, not only contributes to maintenance Intestinal flora balance, improves pig immunity, reduces pig manure stink, moreover it is possible to by adjusting pig
Flora level and enzyme activity level in excrement, which reach, promotes pig manure spontaneous fermentation.
3. selecting component of the dregs of beans as fermentation substrate, it can sufficiently cooperate with selected probiotics, reach good hair
Ferment effect, tunning can meet the nutritional need of pig growth as the high-quality protein source of pig.
4. using the hydrolysis efficiency of a variety of enzyme-linked course of fermentation for effectively improving fermentation substrate and feed.
Detailed description of the invention
Fig. 1 be feeding formula 1. with control formula 1.-the excrement sample compost temperature line chart of test pig 4.；
Fig. 2 be feeding formula 2. with control formula 5.-the excrement sample compost temperature line chart of test pig 8.；
Fig. 3 be feeding formula 1. with control formula 1.-the excrement sample moisture content line chart of test pig 4.；
Fig. 4 be feeding formula 2. with control formula 5.-the excrement sample moisture content line chart of test pig 8.；
Specific embodiment
It in order to enable those skilled in the art to better understand the solution of the present invention, below will be to the skill in the embodiment of the present invention
Art scheme is clearly and completely described, it is clear that and the described embodiment is only a part of the embodiment of the present invention, without
It is whole embodiments.
The general raising pig mode of the embodiment of the present invention is as follows:
The selection grower pigs that health status is good, weight is close, random to be grouped, every group of 20 head growth pig of setting, setting are pre-
Examination phase and experimental period, are tested preliminary trial period 5 days, experimental period 28 days.All test grower pigs free choice feedings, free water, charging side
Formula follows a small amount of multiple principle, feeds daily referring to 6:00,11:00,16:00,21:00 timetable.Immune programme according to
Home court normal immunological program carries out, and pays attention to ventilation and temperature, guarantees that average house is warm (25 ± 3) DEG C.
It is daily to count food consumption and health status during prerun, grasp feed intake, while observe pig behavior and
Health status.It after preliminary trial period, weighs to test pig, and adjust individually, through statistics each group weight without significant difference (P >
0.05) enter formal test when.
The antagonistic experiment of 1 compound probiotic agent strain of embodiment
Using tablet face-off method, activated each bacterial strain on plate is taken, 4 kinds of bacterium are intersected into streak inoculation in common two-by-two
It on nutrient agar, is cultivated 3 days in 40 DEG C of constant temperature and humidity incubators, whether observe has suppressed and send out at right-angled intersection
The phenomenon that raw bacterium colony atrophy and disappearance, to judge whether there is Antagonism between each bacterial strain.The results are shown in Table 1, each bacterial strain it
Between without antagonism, can well be grown under same environment each other, can be used for preparing mix bacterium agent.
The antagonistic experiment result of 4 kinds of probiotics at 1 40 DEG C of table
("+" indicates Antagonism, and "-" indicates no Antagonism)
Embodiment 2 is formulated the production of 1. ecological, environmental protective pannage
It is formulated 1. each raw material of ecological, environmental protective pannage and its accounts for feed total weight percent and listed in table 2, surplus is
Thin chaff:
Table 2 is formulated the 1. component of ecological, environmental protective pannage and its corresponding weight percent
It is formulated the preparation method of 1. ecological, environmental protective pannage:
(1) probiotics and enzyme preparation are added in a certain amount of 40 DEG C of warm water, are stirred evenly, oxygen therapy activation 4 hours；
(2) it is configured to prebiotic bacterium solution according to material water volume ratio 1:0.5 addition water into liquid mixture obtained by step (1),
Then it is inoculated in fermentation vat with fermentation substrate；
(3) sealing pack, the solid anaerobic digestion at 35 DEG C；
(4) fermented mixture of step (3) adds rice chaff to strong fermentation tart flavour is generated thereto.
Embodiment 3 is formulated the production of 2. ecological, environmental protective pannage
It is formulated 2. each raw material of ecological, environmental protective pannage and its accounts for feed total weight percent and listed in table 3, surplus is
Thin chaff:
Table 3 is formulated the 2. component of ecological, environmental protective pannage and its corresponding weight percent
It is formulated the preparation method of 1. ecological, environmental protective pannage:
(1) probiotics and enzyme preparation are added in a certain amount of 40 DEG C of warm water, are stirred evenly, oxygen therapy activation 4 hours；
(2) it is configured to prebiotic bacterium solution according to material water volume ratio 1:0.5 addition water into liquid mixture obtained by step (1),
Then it is inoculated in fermentation vat with fermentation substrate, it is uniform to turn over throwing using turner；
(3) at 35 DEG C, aerobic fermentation, at interval of 12 hours turning and throwing onces after fermentation 24 hours；
(4) after fermentation carries out 72 hours, rice chaff, sealing pack are added thereto.
Comparative example 1
The present embodiment makes pannage according to formula as below, and 1. ecological, environmental protective pannage mentions the formula provided for embodiment 2
For control group:
1. the rice chaff in formula 1. ecological, environmental protective formula for pig forage is replaced with thin chaff, it is formulated remaining component and its weight hundred
Point than and feed production method and embodiment 2 it is completely the same, be labeled as control formula 1..
2. the prebiotic bacterium solution in formula 1. ecological, environmental protective formula for pig forage is replaced with pure water, 1. ecological, environmental protective will be formulated
Fermentation substrate in formula for pig forage replaces with thin chaff, the production method and implementation of remaining component and its weight percent and feed
Example 2 is completely the same, 2. labeled as control formula.
3. the fermentation substrate in formula 1. ecological, environmental protective formula for pig forage is replaced with thin chaff, remaining component and its weight hundred
Point than and feed production method and embodiment 2 it is completely the same, be labeled as control formula 3..
4. being formulated the system of remaining component and its weight percent and feed using the prebiotic bacterium solution for not containing lactobacillus plantarum
Make method and embodiment 2 is completely the same, 4. labeled as control formula.
Comparative example 2
The present embodiment makes pannage according to formula as below, and 2. ecological, environmental protective pannage mentions the formula provided for embodiment 3
For control group:
1. the rice chaff in formula 2. ecological, environmental protective formula for pig forage is replaced with thin chaff, it is formulated remaining component and its weight hundred
Point than and feed production method and embodiment 3 it is completely the same, be labeled as control formula 5..
2. the prebiotic bacterium solution in formula 2. ecological, environmental protective formula for pig forage is replaced with pure water, 1. ecological, environmental protective will be formulated
Fermentation substrate in formula for pig forage replaces with thin chaff, the production method and implementation of remaining component and its weight percent and feed
Example 3 is completely the same, 6. labeled as control formula.
3. the fermentation substrate in formula 2. ecological, environmental protective formula for pig forage is replaced with thin chaff, remaining component and its weight hundred
Point than and feed production method and embodiment 3 it is completely the same, be labeled as control formula 7..
4. being formulated the system of remaining component and its weight percent and feed using the prebiotic bacterium solution for not containing lactobacillus plantarum
Make method and embodiment 3 be completely the same, 8. labeled as control formula.
Embodiment 4
The setting of 4.1 experimental groups
Health status is good, weight is close grower pigs 120 are selected, random to be grouped, every group of 20 head growth pig of setting, point
Not with the pannage feeding of 10 kinds of different formulations of embodiment 2, embodiment 3, comparative example 1 and comparative example 2 preparation.If
It sets preliminary trial period and experimental period, tests preliminary trial period 5 days, experimental period 28 days.All test grower pigs free choice feedings, free water, add
Material mode follows a small amount of multiple principle, feeds daily referring to 6:00,11:00,16:00,21:00 timetable.Immune programme
It is carried out according to home court normal immunological program, pays attention to ventilation and temperature, guarantee that average house is warm (25 ± 3) DEG C.
It is daily to count food consumption and health status during prerun, grasp feed intake, while observe pig behavior and
Health status.It after preliminary trial period, weighs to test pig, and adjust individually, through statistics each group weight without significant difference (P >
0.05) enter formal test when.
The acquisition of 4.2 excrement samples
When test was carried out to 25 days, receives excrement 100g daily as unit of colony house, 10% dilute sulfuric acid 10mL is added, is stored in
In 4 DEG C of refrigerators.It is continuous to receive excrement 3 days and mixed as unit of colony house, it is dried in 60-70 DEG C of baking oven, it is small to take out sample resurgence 24
When, sample comminution is spare.
When test was carried out to 28 days, the acquisition of microorganism excrement sample is carried out to grower pigs as unit of colony house, with 10mL sterilize from
Heart pipe acquires fresh free of contamination excrement sample, takes in -20 DEG C of refrigerators immediately and saves.
The upgrowth situation of 5 test pig of embodiment
Respectively for using formula 1., control formula 1., formula 2. with control formula 5. corresponding pannage feeding experiment
Pig is weighed.It weighs on an empty stomach morning the 1st day on-test and the 28th day morning of off-test, and by computing repeatedly feed
Consumption, and calculate:
Average daily feed intake=(initial doses-residue doses)/(test number of days × pig's head number)；
Average weight increasing a day=(terminal weight-original body mass)/(test number of days × pig's head number)；
Feed-weight ratio=average daily feed intake/Average weight increasing a day.
The upgrowth situation statistical form of 4 test pig of table
Test result (table 4) shows that all test grower pigs original body mass differences are not significant (P > 0.05).Feed basis
The average daily feed intake of test pig and Average weight increasing a day for being formulated the pannage 1. prepared are all higher than what 1. feeding was prepared according to control formula
The test pig of pannage, compares the feed-weight ratio being calculated from the data that two groups of test pigs acquire, 1. feeding is prepared according to formula
Pannage test pig it is higher.The average daily feed intake of test pig and Average weight increasing a day of 2. pannage that feeding is prepared according to formula are all
Higher than the test pig of 5. pannage that feeding is prepared according to control formula, comparison is calculated from the data that two groups of test pigs acquire
Feed-weight ratio, feeding is suitable according to the 2. 5. test pig of pannage that formula is prepared with control formula.Illustrate, using 20-30%
Rice chaff does not bring negative influence to the upgrowth situation of test pig as pannage component, does not also reduce the appetite of test pig.
The measurement of microorganism and enzyme activity in 6 excrement sample of embodiment
1. the measurement of microorganism in excrement sample
It takes 1g excrement sample in sterile test tube in superclean bench, PBS buffer solution 9mL is added, magnetic force oscillator vibrates 5 points
Clock, this liquid are 10-1Dilution, absorption this solution of 1mL, which is loaded on to fill in 9mL sterile PBS buffer test tube, carries out 10-2Dilution, vibration
It swings 5 minutes, and successively carries out 10-3-10-6It dilutes again.It is measured using plate count method by the large intestine in diluted excrement sample
Bacillus, bacillus, saccharomycete, actinomyces and lactobacillus plantarum.
Influence of the table 4 using different formulations pannage feeding experiment pig to flora in excrement sample
2. the measurement of amylase, neutral proteinase, urase, cellulase in excrement
By sample and water by weight 1:6 dilution proportion, 0.45 μm of filtering, take filtrate illustrate to measure according to kit it is various
Enzyme activity.Kit builds up Bioengineering Research Institute purchased from Nanjing.
Influence of the table 5 using different formulations pannage feeding experiment pig to enzyme activity in excrement sample
According to the data that table 4,5 provides, to add prebiotic bacterium solution or feed pig using fermentation substrate as the pannage of component
Only, the flora and enzyme activity in pig manure can be significantly affected: (1) improving the content of lactic acid bacteria in excrement sample；(2) it reduces in excrement sample
The content of Escherichia coli；(3) enzyme activity of the cellulase, neutral proteinase, urase and amylase that improve in excrement sample is shown.Feed
In probiotics can be functioned in pig enteron aisle anaerobic environment, by inhibit pathogenic bacteria breeding, promote beneficial bacterium expand it is numerous,
So that the E. CoIi content in corresponding excrement sample reduces, content of lactic acid bacteria increases.Fermentation substrate as pannage component
It effectively ferments under the action of probiotics and enzyme preparation, it is a variety of to produce much starch enzyme, protease, urase and cellulase etc.
Enzyme preparation still can play a role by the anaerobic environment of pig gastrointestinal tract in excrement sample composting fermentation process.
The measurement of skatole level in 7 excrement sample of embodiment
The measurement of skatole level in excrement: taking 2g excrement, and Manual low-temp is homogenized after methanol is added by weight 1:9, takes
Slurries carry out scatol measurement using HPLC method by food quality inspection center, the Ministry of Agriculture.
Influence of the table 6 using different formulations pannage feeding experiment pig to skatole level in excrement sample
As shown in Table 6, respectively compared with respective control formula, using formula, 1. 2. corresponding pannage is fed with formula
The pig manure drained of test pig in skatole level significantly reduce.Wherein, comparison feeding formula 1. match by pannage and control
The square 4. corresponding excrement sample of pannage test pig, 2. 8. pannage test pig is corresponding for pannage and control formula for comparison feeding formula
Excrement sample, it is known that, the prebiotic bacterium solution containing lactobacillus plantarum is added in pannage can be effectively reduced the skatole level in excrement sample.
8 excrement sample of embodiment fermentation situation
The heap body base portion length and width of compost respectively may be about 1.9m, 1.2m, the high 0.8m of heap.When compost starts, compost excrement sample is adjusted
Moisture content to 60% or so.Compost use artificial turning, every 3 days 1 time
It is primary every sampling in 5 days (6 hours after material mixing) and later on the day of compost, it is excavated on front side of each compost
High, wide is respectively 0.7m, the sample log of 0.4m, from top to bottom vertical sampling thickness about 3-5cm, each sampling amount about 1kg.First
It is secondary to be sampled as the preceding sample that ferments, it is sampled as sample after fermentation for the last time.Every morning 8:30 is (deep by being inserted at the top of heap body
Thermometer 25cm) records compost temperature, while measuring ambient temperature and humidity.
According to Fig. 1 and Fig. 2, after starting compost, the temperature of each experimental group excrement sample is all increased rapidly, pair in addition to being free of rice chaff
According to formula 1. pannage and control formula 5. outside the corresponding excrement sample of pannage, each experimental group excrement sample after compost starts 10 days all into
High temperature fermentation stage (50 DEG C of >) is entered, has illustrated that the pig fed with the pannage containing rice chaff is conducive to pig manure caused by it
From being advanced into hot fermentation state.Respectively compared with respective control formula, 1. 2. pannage is corresponding with formula for pannage for formula
Excrement sample heating rate it is most fast and increasing extent of temperature is maximum.According to Fig. 3 and Fig. 4, after compost starts, the moisture content of each group excrement sample
All on a declining curve, the dehydrating effect of excrement sample and the correlation of excrement sample temperature are relatively high, corresponding to be formulated 1. pannage and formula 2.
The fermentation temperature of the excrement sample of pannage, just at zooming trend, and maintains high whithin a period of time after compost starts
Temperature, therefore, the moisture content for corresponding to excrement sample decline rapidly.In addition, produced with the test pig of the pannage feeding containing rice chaff
Excrement sample is in loose shape after entering composting period, is conducive to the fermentation calefaction and dehydration by evaporation of microorganism in excrement sample.According to this reality
Data measured by example are applied, the rice chaff, probiotics, enzyme preparation and fermentation substrate in pannage can promote to feed to some extent
Raise pig caused by excrement sample fermentation calefaction and dehydration by evaporation, promote effect within compost fermentation 5 to 20 days periods the most
Obviously.
To sum up, feeding is formulated excrement sample caused by the 1. test pig of the ecological, environmental protective pannage with formula 2. and is easy compost hair
Ferment, can also high-efficiency fermenting even if no longer addition external source bacterium in compost fermentation.
Finally it should be noted that the above embodiments are merely illustrative of the technical scheme of the present invention and are not intended to be limiting thereof, to the greatest extent
Invention is explained in detail referring to above-described embodiment for pipe, it should be understood by a person of ordinary skill in the art that technology
Personnel read present specification after still can with modifications or equivalent substitutions are made to specific embodiments of the invention, but this
A little modifications are changed all without departing from the present patent application accompanying claims protection scope.
Claims (10)
1. a kind of ecological, environmental protective pannage, which is characterized in that including below according to the raw material for accounting for feed total weight percent: 20-
30% rice chaff, 5-20% fermentation substrate, 0.015-0.18% probiotics；The probiotics includes 0.005-0.06% gemma bar
Bacterium, 0.005-0.06% saccharomycete and 0.005-0.06% actinomyces.
2. ecological, environmental protective pannage as described in claim 1, it is characterised in that: the probiotics further includes accounting for feed total weight hundred
Divide than 0.005-0.06% lactic acid bacteria.
3. ecological, environmental protective pannage as claimed in claim 2, it is characterised in that: the lactic acid bacteria is lactobacillus plantarum.
4. ecological, environmental protective pannage as described in claim 1, it is characterised in that: further include accounting for feed total weight percent
0.0015-0.018% enzyme preparation, the enzyme preparation is selected from cellulase, Niu Laisi enzyme and Jie Fu can one of enzyme or a variety of.
5. ecological, environmental protective pannage as claimed in claim 4, which is characterized in that the enzyme preparation is by below according to accounting for the enzyme system
The group of agent total weight percent is grouped as: 20-40% cellulase, 20-40% button Lay think enzyme and 20-40% outstanding person's richness can enzyme.
6. ecological, environmental protective pannage as described in claim 1, which is characterized in that the fermentation substrate includes described according to accounting for below
The raw material of fermentation substrate total weight percent: 80-95% dregs of beans, 5-20% wheat-middlings.
7. ecological, environmental protective pannage as claimed in claim 6, which is characterized in that be prepared as follows:
(1) probiotics and the enzyme preparation are cured by oxygen therapy to activation in 35-45 DEG C of water；
(2) it is configured to prebiotic bacterium solution according to material water volume ratio 1:0.5 addition water into liquid mixture obtained by step (1), then
It is inoculated into the fermentation substrate；
(3) at 35 DEG C, solid anaerobic digestion；
(4) after the fermented mixture of step (3) is complete, the rice chaff is added thereto.
8. ecological, environmental protective pannage ecological, environmental protective pannage as described in claim 1, which is characterized in that the fermentation substrate includes
Below according to the raw material for accounting for the fermentation substrate total weight percent: 55-70% dregs of beans, 20-30% cotton dregs, 1-5% mountain flour,
0.5-2% calcium monohydrogen phosphate, 0.5-2% sodium chloride and the more mines of 0.5-2% piggy.
9. ecological, environmental protective pannage as claimed in claim 8, which is characterized in that be prepared as follows:
(1) probiotics and the enzyme preparation are cured by oxygen therapy to activation in 35-45 DEG C of water；
(2) it is configured to prebiotic bacterium solution according to material water volume ratio 1:0.5 addition water into liquid mixture obtained by step (1), then
It is inoculated into the fermentation substrate；
(3) at 35 DEG C, aerobic fermentation, at interval of 12 hours turning and throwing onces after fermentation 24 hours；
(4) after fermentation carries out 72 hours, the rice chaff is added into gained mixture.
10. a kind of method for making pig manure voluntarily ferment, it is characterised in that: using such as any one of claim 1,2,3,4,5,6,8
The ecological, environmental protective pannage feeds pig.
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| 《农林科学实验》编辑部编: "《家畜繁殖和饲养管理》", 31 January 1985, 第122,123页 * |
| 朱飞，等: "《养猪生产技术问答 第2版》", 29 February 1984, 上海科学出版社 * |
| 浙江省农学会主编: "《农业生产科技问答 畜牧兽医及小动物饲养分册》", 31 December 1982, 浙江科学技术出版社 * |
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