CN110140663A - A kind of environmentally friendly pig raising fermenting bed padding and preparation method thereof - Google Patents
A kind of environmentally friendly pig raising fermenting bed padding and preparation method thereof Download PDFInfo
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- 241000894006 Bacteria Species 0.000 claims abstract description 34
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 28
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 20
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
- A01K1/0152—Litter
- A01K1/0154—Litter comprising inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
- A01K1/0152—Litter
- A01K1/0155—Litter comprising organic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Environmental Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Biodiversity & Conservation Biology (AREA)
- General Health & Medical Sciences (AREA)
- Animal Husbandry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Botany (AREA)
- Inorganic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The environmentally friendly pig raising fermenting bed padding of one kind is prepared using fermenting agent and fermentation substrate as raw material by heap fermentation;Fermentation substrate includes following parts by weight material: 15-35 parts of cotton stalk, 10-40 parts of vinegar grain, 10-40 parts of peanut shell, 10-20 parts of Pleurotus eryngii mushroom bran;The parts by weight of fermenting agent are 1-5 part, living bacteria count >=10 in fermenting agent8Cfu/g, efficient bacterium group therein are combined into bacillus+saccharomycete+lactic acid bacteria+actinomyces, and meet following living bacteria count ratio: bacillus: saccharomycete: lactic acid bacteria: actinomyces=1-5:0.5-1.5:1-3:1-3.The feeding environment that live pig can be effectively improved using fermenting bed padding obtained after fermentation substrate of the invention and fermenting agent progress heap fermentation, is reduced pig house peculiar smell, improves the productivity effect of live pig.
Description
Technical field
The invention belongs to technical field of animal husbandry, and in particular, to a kind of environmentally friendly pig raising fermentation mattress
Material and preparation method thereof.
Background technique
Pig raising is the important component of China's agricultural production, is the important means that increasing peasant income are got rich, pork annual output
50000000 t or so, rank first in the world.It is counted according to the Ministry of Agriculture, in October, 2011, whole nation live pig livestock on hand was up to 4.71 hundred million.However,
While pig breeding industry is grown rapidly, problem of environmental pollution caused by pig raising waste excretion, which also becomes, hinders China's live pig industry can
An important factor for sustainable development.Fermentation bed to raise pig technology is to comprehensively utilize microbiology, life using completely new natural agriculture theory
State, Fermentation Engineering principle, a kind of environmental protection using active function microorganism as matter energy " conversion maincenter ", safely, have
The ecologically raising pigs mode of effect.Fermentation bed to raise pig technology is a large amount of to be inoculated with as a kind of pollution-free, zero-emission organic agriculture technology
Probiotics and pig manure in normal flora constitute fermentation bed dominant microflora, with crops such as sawdust, wheat bran, rice husks according to certain
Ratio cooperation be used as padding, after fermented, in fermentation bed ira situ degradation, consumption fecaluria.
In traditional cement floor pig house, odorant caused by pig manure fermentation up to more than 160, these odorants
Mainly include volatile fatty acid, phenols, indoles, ammonia, volatile amine and sulfur-containing compound etc., can reduce pig resistance and
Immunity, weakens the metabolism degree of pig, so that production capacity be caused to decline, improves to the neurological susceptibility of disease.People lives in for a long time
In the stench environment on pig farm periphery, it is also more susceptible to suffer from the respiratory diseases such as tracheitis, bronchitis and pneumonia.
Microbial fermentation bed pig house is Full-open pig house, and the excreta of pig is dropped rapidly by the bacterium in padding as nutrition
Solution, digestion, pig house are interior without obvious peculiar smell.The fermentation bed of good course of fermentation can reduce urea, ammonia, nitrous oxide, hydrogen sulfide,
The stink substancess such as indoles and 3-methyl indols generate and volatilization, reduce many gases beastly and generate.
The source of fermenting bed padding and selection are one of the core link of fermentation bed to raise pig technology, classical fermenting bed padding system
Need to consume a large amount of husk and wood sawdust, husk and wood sawdust resource are limited, constrain the popularization of fermenting bed padding technology
With application.
Summary of the invention
The object of the present invention is to provide a kind of environmentally friendly pig raising fermenting bed paddings and preparation method thereof, on solving
State at least one of technical problem.
According to an aspect of the present invention, a kind of environmentally friendly pig raising fermenting bed padding is provided, using fermenting agent
With fermentation substrate as raw material, prepared by heap fermentation;Fermentation substrate includes following parts by weight material: cotton stalk 15-35
Part, 10-40 parts of vinegar grain, 10-40 parts of peanut shell, 10-20 parts of Pleurotus eryngii mushroom bran;The parts by weight of fermenting agent are 1-5 part, fermentation
Living bacteria count >=10 in microbial inoculum8Cfu/g, efficient bacterium group therein are combined into bacillus+saccharomycete+lactic acid bacteria+unwrapping wire
Bacterium, and meet following living bacteria count ratio: bacillus: saccharomycete: lactic acid bacteria: actinomyces=1-5:0.5-1.5:1-3:1-3.
Preferably, the parts by weight of fermenting agent are 3 parts, further include 3-5 parts of brown sugar;Fermentation substrate include 25 parts of vinegar grain,
30 parts of peanut shell, 15 parts of Pleurotus eryngii mushroom bran, 0-10 part of calcium monohydrogen phosphate.
Preferably, the living bacteria count in fermenting agent meets: bacillus: saccharomycete: lactic acid bacteria: actinomyces=2:1:
2:1.
Preferably, bacillus is selected from bacillus subtilis, bacillus licheniformis, bacillus coagulans, waxy gemma bar
One of bacterium, bacillus pumilus, clostridium butyricum are a variety of.
Preferably, bacillus is made of bacillus subtilis and bacillus coagulans, and meets following living bacteria count
Than: bacillus subtilis: bacillus coagulans=1:1.
According to another aspect of the present invention, a kind of preparation side of above-mentioned environmentally friendly pig raising fermenting bed padding is provided
Method, comprising the following steps: fermenting agent is added 20-30h of activation culture in brown sugar water with the amount of 5wt%, obtained by step (1)
Activate zymocyte liquid;Step (2), material needed for weighing fermentation substrate stir and evenly mix, obtain fermentation substrate;Step (3), will
Activate zymocyte liquid inoculation fermentation substrate;Step (4) adds water to adjust fermentation substrate, makes the water content 55-of fermentation substrate
65%;Step (5), heap fermentation 6-8 days.
Preferably, in step (2), the parts by weight of cotton stalk and calcium monohydrogen phosphate is controlled, the carbon nitrogen of fermentation substrate is made
Than C/N=25-40:1, carbon-phosphorus ratio C/P=60-80:1.
Preferably, in step (4), after the water content adjusting for completing fermentation substrate, the pH value of fermentation substrate is adjusted, is made
Its pH=7-9.
Preferably, in step (4), the water content of fermentation substrate is controlled 60% ± 1.5%.
Preferably, in step (5), when fermentation temperature rises to not less than 50 DEG C and continues 2 days, to fermentation system
Material carries out continuing to ferment after stirring stirring.
Microbial population composition in the pairs of fermentation bed of the group of fermenting bed padding is closely related, and the present invention is using present invention choosing
Selecting vinegar grain, peanut shell and Pleurotus eryngii mushroom bran cooperates other auxiliary materials as fermenting bed padding is prepared, with cotton stalk, vinegar grain, peanut
The fermentation substrate of shell and Pleurotus eryngii mushroom bran composition can provide organic matter abundant for microorganism therein, by above-mentioned fermentation bottom
Fermenting bed padding made of after object heap fermentation meets nutrition complement principle, pig by arch food padding, can absorb it is abundant,
Balanced nutrition.And respectively possessed peculiar fragrance being capable of provocative test for the vinegar grain and Pleurotus eryngii mushroom bran for forming fermenting bed padding
The appetite of pig.In addition, Pleurotus eryngii mushroom bran stimulation live pig secretes various digestive ferments, improves live pig and feed is digested and absorbed
Rate.And vinegar grain can also play the role of inhibiting harmful pathogen, reduce the etiology in pig house.The fermentation limited by the present invention
Fermenting substrate works well, and higher environment can be provided for live pig using the fermentation bed of the fermenting bed padding building thus prepared
Temperature increases the production welfare of live pig.
In addition, the present invention forms selection according to the fermentation substrate for preparing fermenting bed padding there is specific efficient bacterium to combine
Fermenting agent, fermenting agent is added in fermentation substrate and carries out heap fermentation jointly, a variety of probiotics in fermenting agent
Synergy, can keep higher activity in fermentation system, and organic substance, which is converted into, in promotion fermentation substrate is conducive to give birth to
The nutriment of pig growth and development inhibits pathogenic bacteria sending out additionally it is possible to play the role of adjusting fermentation bed body flora composition
It is proliferated in ferment bed, makes probiotics rich in fermentation bed.The abundant probiotics of fermentation bed body can effectively decompose pig excrement
Dirt improves pig house environment, in addition, encircleing food padding by live pig, the abundant probiotics in padding enters in live pig body, can cause
Chitling road anaerobic environment reduces pH value, activates pepsin, some probiotics therein itself also can produce a variety of hydrolases
Class and probiotic, promote the digestion and absorption of nutriment, to improve the production performance of pig.
Generally speaking, the present invention is carried out by being defined to the fermentation substrate and fermenting agent that prepare fermenting bed padding
Fermenting bed padding obtained can effectively improve the feeding environment of live pig after heap fermentation, reduce pig house peculiar smell, improve live pig
Productivity effect.
Specific embodiment
It in order to enable those skilled in the art to better understand the solution of the present invention, below will be to the skill in the embodiment of the present invention
Art scheme is clearly and completely described, it is clear that and the described embodiment is only a part of the embodiment of the present invention, without
It is whole embodiments.
Embodiment 1
6 test groups are arranged in the present embodiment, successively labeled as according to processing, processing I, processing II, processing III, processing IV and place
Reason V carries out feeding live pig test.Wherein, I-V is handled for the feeding experiment pig in the pig house for being equipped with fermentation bed, at control
Reason is the feeding experiment pig in traditional cement floor pig house.
1. making fermenting bed padding
Each processing group prepares fermentation substrate using corresponding material according to table 1, the C/N=30 for the fermentation substrate thus matched
± 2, carbon-phosphorus ratio C/P=72 ± 2.
Raw material needed for table 1 prepares the fermentation substrate of each test group and its parts by weight (part)
In addition to above-mentioned fermentation substrate, the raw material that each test group of the present embodiment prepares fermenting bed padding further includes fermenting agent 3
Part and 4 parts of brown sugar.Effective total viable count >=10 in the fermenting agent8Cfu/g, effective viable bacteria is by bacillus subtilis, solidifying
Bacillus, saccharomycete, lactic acid bacteria and actinomyces composition are tied, viable count therein is than meeting: bacillus subtilis: condensation gemma
Bacillus: saccharomycete: lactic acid bacteria: actinomyces=1:1:1:2:1.
The step of each test group prepares fermenting bed padding is as follows:
(1) above-mentioned fermenting agent is added brown sugar water activation culture 24 hours with the amount of 5wt%, obtains zymocyte liquid;
(2) it weighed vinegar grain, peanut shell, Pleurotus eryngii mushroom bran, cotton stalk and calcium monohydrogen phosphate will mix according to parts by weight,
It is stirred well to and is uniformly distributed, obtain fermentation substrate;
(3) zymocyte liquid that activation is sprayed into fermentation substrate stirs fermentation substrate while spraying;
(4) plus the water content of water adjusting fermentation substrate is to 60% ± 1.5%, then adjusts fermentation using acidity-basicity regulator
The pH value of substrate makes its pH=7.6;
(5) heap fermentation 7 days, when fermentation temperature rises to not less than 50 DEG C and continues 2 days, to the material of fermentation system
It carries out continuing to ferment after stirring stirring.
2. constructing fermentation bed
Adding water to adjust fermenting bed padding to the water content for completing to ferment is 50%, utilizes acidity-basicity regulator adjustment fermentation bed
The pH value of padding is to pH=7.8.Fermenting bed padding Jing Guo above-mentioned processing is spread out in culturing area, laying with a thickness of
70cm, next day is into pig.
3. feeding management
It is " Ternary Pig " the three way cross live pig 60 of 30kg or so that test pig, which selects weight, and parity is consistent, kind phase
Together, age in days is close, according to the fifty-fifty group technology of male and female, is randomly divided into 6 groups, every group of 10 repetitions, each repetition 1 is tested
Pig.Test pig house sets 7 days preliminary trial periods before into Antiepidemic sterilizing work is carried out before pig, testing formal start, during prerun, according to
The normal feeding management process of farm carries out the work such as vaccine injection, expelling parasite and earmarking to test pig.Experimental period is 60 days,
During test: the test pig of control treatment is freely eaten in cement floor pig house, drinks water, and does clear mode and cleans excrement;Processing
I-V test pig is freely eaten in fermentation bed, drinks water and defecation, feeds the composition of daily ration used by six groups of test pigs such as
Shown in table 2.It in experimental period, turns over take off I-V fermentation bed surface layer padding of processing daily, pad the fecaluria for being in padding surface layer originally
Material is covered and is buried, and turns over comprehensively take off after the 50cm of upper layer that monthly thoroughly to plough deeply padding primary weekly.Whenever padding height declines about 10cm, addition
The fermenting bed padding of same composition supplements, and when charging, virgin material and old material will be sufficiently mixed to reach strain and be uniformly dispersed, and be adjusted simultaneously
Moisture content has been saved to 50%.
The daily ration of 2 feeding experiment pig of table forms
4. index test
4.1 production performance
Start day and closing day in experimental period, weighs on an empty stomach respectively at early morning to each group test pig, record examination in experimental period
The feed intake of pig is tested, average feed intake, average daily gain and feed-weight ratio are calculated.
The temperature test of 4.2 fermenting bed paddings
Entering the 10th, 20,30,40,50 and 60 day of experimental period in each test group house, by the selection of uniform stationing principle
East, West, South, North, in 5 points first with about 20cm in iron rod insertion padding then use thermometer measure temperature, the data obtained asks
Average value.
The monitoring of 4.3 pig house environments
Entering the 60th day of experimental period, in each test group house, by uniform stationing principle select East, West, South, North, in 5
It is a, at away from ground 0.3~0.5m height, in morning 6:00 or so timing portable ammonia analyzer (S-450, the U.S.
Sai Li Mann) and hydrogen sulfide detector (HL-240, Dreager Medical Devices (Shanghai) Co., Ltd.) measure respectively ammonia and
The concentration of hydrogen sulfide detects total suspension with particle concentration monitor (ZH6822, Beijing Zhong Huitiancheng Science and Technology Ltd.)
Grain concentration.Total number of bacteria measurement using plate exposure method, by nutrient agar plate opening be individually placed to each test group She Neidong,
West, south, north, in 5 angles cover plate lid after 5min is placed in exposure, take back laboratory, 37 DEG C of culture 48h, counting plate
Upper bacteria colony count, and it is used into Austria Mei Liang Si base formula scales:
Y=(A × 50000)/(S × t);
In formula, y is total number of bacteria (CFU/m in air3);A is average bacteriological clump count on plate;S is platen area
(cm2);T is exposure duration (min).
5. test result
5.1 production performance test results
Do not occur swine fever case in experimental period without there is dead example yet.As shown in table 3, the average feeding of control treatment
Amount, average daily gain are significantly relatively low, and its corresponding feed-weight ratio has reached the maximum value in all test groups.Control treatment exists
Feeding experiment pig in traditional cement floor pig house, sanitary condition is relatively poor, influences the appetite of test pig, also reduces test
The nutrition conversion ratio of pig, compared with each processing group, the yard feeding pattern of control treatment is unfavorable for the growth and development of test pig.Each processing
The pig of group has all formed the habit that food fermenting bed padding is directly discharged feces and encircleed in fermentation bed after entering experimental period.And
The feed-weight ratio of processing I is substantially less than other processing groups, and leading to such reason may be the original for preparing fermenting bed padding for handling I
Expect that type is relatively fewer, nutriment is more single.In general, the production performance for handling V test pig is best, processing V
The raw material type of fermenting bed padding is used to prepare compared with horn of plenty, and vinegar grain included in it and Pleurotus eryngii mushroom bran are respectively had
The peculiar fragrance having is capable of the appetite of provocative test pig.
The production performance of 3 the present embodiment test pig of table
The temperature test of 5.2 fermenting bed paddings
The fermenting bed padding temperature test result of processing I-V is as shown in table 4.The heating rate of the fermenting bed padding of processing I
More slowly, the 30th day after entering experimental period, padding temperature is not higher than 35 DEG C, and in entire experimental period, maximum temperature is not high
In 40 DEG C.It into after experimental period, handles V fermenting bed padding temperature and increases rapidly, entering the 30th day experimental period to experimental period
At the end of, temperature maintains in the range of 45 DEG C ± 2 DEG C.Therefore, test pig lies down in the fermentation bed of processing V, leads to it
Abdomen sendible temperature is higher than the test pig of other processing groups, to increase the test pig welfare of processing V.
Temperature test result (DEG C) at the fermenting bed padding depth 20cm of the processing of table 4 I-V
5.3 pig house environment monitoring results
The results are shown in Table 5 for control treatment and the pig house environment index test of processing I-V.Have in the pig house of control treatment
Pungent excrement stink, ammonia concentration, concentration of hydrogen sulfide are significantly higher than I-V group of processing.Compared with control treatment, processing I-V
It is laid with fermentation bed in pig house, can be effectively reduced concentration of suspended particles and total number of bacteria in pig house.Due to pig house inner ring
Border is improved, and the state of mind of the test pig of processing I-V is better than the test pig of control treatment.
5 pig house environment quality-monitoring result of table
According to the feeding experiment of the present embodiment setting as a result, being formed using handling V corresponding fermentation substrate raw material as this
Embodiment prepares the best fermentation substrate raw material composition of fermenting bed padding.
Embodiment 2
3 test groups are arranged in the present embodiment, successively labeled as according to processing, processing A, processing B, carry out feeding live pig test.
Wherein, processing A and processing B is the feeding experiment pig in the pig house for being equipped with fermentation bed, and control treatment is in traditional cement floor
Feeding experiment pig in pig house.
1. making fermenting bed padding
Processing A and the fermentation bottom that processing B prepares the fermentation substrate of fermenting bed padding use and the processing V of embodiment 1 uses
Object composition is identical.In addition to above-mentioned fermentation substrate, it further includes fermenting agent 3 that the present embodiment processing A, which prepares the raw material of fermenting bed padding,
Part and 4 parts of brown sugar.Effective viable bacteria composition of the fermenting agent is identical as the fermenting agent that the processing V of embodiment 1 uses.Handle A
According to
The fermenting bed padding that processing A is used is prepared in accordance with the following steps:
(1) above-mentioned fermenting agent is added brown sugar water activation culture 24 hours with the amount of 5wt%, obtains zymocyte liquid;
(2) it weighed vinegar grain, peanut shell, Pleurotus eryngii mushroom bran, cotton stalk and calcium monohydrogen phosphate will mix according to parts by weight,
It is stirred well to and is uniformly distributed, obtain fermentation substrate;
(3) zymocyte liquid that activation is sprayed into fermentation substrate stirs fermentation substrate while spraying;
(4) plus the water content of water adjusting fermentation substrate is to 60%, then adjusts fermentation substrate using acidity-basicity regulator
PH value makes its pH=7.6;
(5) heap fermentation 7 days, when fermentation temperature rises to not less than 50 DEG C and continues 2 days, to the material of fermentation system
It carries out continuing to ferment after stirring stirring.
The fermenting bed padding that processing B is used is prepared in accordance with the following steps:
(1) it weighed vinegar grain, peanut shell, Pleurotus eryngii mushroom bran, cotton stalk and calcium monohydrogen phosphate will mix according to parts by weight,
It is stirred well to and is uniformly distributed, obtain fermentation substrate;
(2) plus the water content of water adjusting fermentation substrate is to 60%, then adjusts fermentation substrate using acidity-basicity regulator
PH value makes its pH=7.6;
(3) heap fermentation 7 days, when fermentation temperature rises to not less than 50 DEG C and continues 2 days, to the material of fermentation system
It carries out continuing to ferment after stirring stirring.
2. constructing fermentation bed
Adding water to adjust fermenting bed padding to the water content for completing to ferment is 50%, utilizes acidity-basicity regulator adjustment fermentation bed
The pH value of padding is to pH=7.8.Fermenting bed padding Jing Guo above-mentioned processing is spread out in culturing area, laying with a thickness of
70cm, next day is into pig.
3. feeding management
It is " Ternary Pig " the three way cross live pig 30 of 30kg or so that test pig, which selects weight, and parity is consistent, kind phase
Together, age in days is close, according to the fifty-fifty group technology of male and female, is randomly divided into 3 groups, every group of 10 repetitions, each repetition 1 is tested
Pig.Test pig house sets 7 days preliminary trial periods before into Antiepidemic sterilizing work is carried out before pig, testing formal start, during prerun, according to
The normal feeding management process of farm carries out the work such as vaccine injection, expelling parasite and earmarking to test pig.Experimental period is 60 days,
During test: the test pig of control treatment is freely eaten in cement floor pig house, drinks water, and does clear mode and cleans excrement;Handle A
Is freely eaten, drink water and defecation in fermentation bed with the test pig of processing B, daily ration composition used by three groups of test pigs of feeding
As shown in the table 2 of embodiment 1.It in experimental period, turns over take off processing A and processing B fermentation bed surface layer padding daily, make originally in padding
The fecaluria on surface layer is covered by padding to be buried, and turns over comprehensively take off after the 50cm of upper layer that monthly thoroughly to plough deeply padding primary weekly.Whenever padding height
Decline about 10cm, the fermenting bed padding supplement of same composition is added, when charging, virgin material and old material will be sufficiently mixed to reach strain
It is uniformly dispersed, while regulating moisture content to 50%.
4. index test
4.1 production performance
Start day and closing day in experimental period, weighs on an empty stomach respectively at early morning to each group test pig, record examination in experimental period
The feed intake of pig is tested, average feed intake, average daily gain and feed-weight ratio are calculated.
The measurement of 4.2 physiochemical indices
Off-test the last week randomly selects 3 test pigs in every group and carries out the blood sampling of empty stomach vena cava anterior, 3000r/
Min centrifugation 5min takes serum to measure for Serum Indexes.Growth hormone (GH), cortisol (Cor), heat shock protein70 (HSP-
70) kit measurement (enzyme-linked immunization) produced by Wuhan Bei Yinlai Biotechnology Co., Ltd.
5. test result
As shown in table 6, compared with control treatment, processing A and processing B use the pig house feeding experiment pig for being laid with fermentation bed,
The production performance of test pig can comprehensively be improved.However, adding zymophyte when preparing fermenting bed padding compared with handling B
The production performance of the processing A of agent has obtained further raising.
The production performance of 6 the present embodiment test pig of table
As shown in table 7, compared with control treatment, processing A is reduced in test pig blood sample with the processing respectively different degree of B
Cor, HSP70 and AKP content, Cor, HSP70 and AKP all be reflect live pig stress index.Cor, HSP70 and AKP's
The stress that content shows that test pig is subject to during the growth process is slight, and the nutrient of pig feeding is bad for resisting extraneous factor
The ratio of influence reduces, the nutrients ratio relative increase for growth.In three groups of test groups of the present embodiment, the examination of A is handled
The content of Cor, HSP70 and AKP for testing in pig blood sample respectively reach minimum between the group of corresponding index, in conjunction with handling A's in table 6
Feed-weight ratio reaches the minimum value in all three groups of experimental groups, and fermenting agent system used by the present embodiment is added into fermentation substrate
Standby fermenting bed padding, can further improve the productivity effect of test pig.
The Serum Indexes of 7 the present embodiment test pig of table are tested
Finally it should be noted that the above embodiments are merely illustrative of the technical scheme of the present invention and are not intended to be limiting thereof, to the greatest extent
Invention is explained in detail referring to above-described embodiment for pipe, it should be understood by a person of ordinary skill in the art that technology
Personnel read present specification after still can with modifications or equivalent substitutions are made to specific embodiments of the invention, but this
A little modifications are changed within all without departing from the present patent application accompanying claims protection scope.
Claims (10)
1. a kind of environmentally friendly pig raising fermenting bed padding, it is characterised in that: using fermenting agent and fermentation substrate as former
Material, prepares by heap fermentation;
The fermentation substrate includes following parts by weight material: 15-35 parts of cotton stalk, 10-40 parts of vinegar grain, 10-40 parts of peanut shell,
10-20 parts of Pleurotus eryngii mushroom bran;
The parts by weight of the fermenting agent are 1-5 part, living bacteria count >=10 in the fermenting agent8Cfu/g, it is therein
Efficient bacterium group is combined into bacillus+saccharomycete+lactic acid bacteria+actinomyces, and meets following living bacteria count ratio: the gemma bar
Bacterium: the saccharomycete: the lactic acid bacteria: the actinomyces=1-5:0.5-1.5:1-3:1-3.
2. environmentally friendly pig raising fermenting bed padding as described in claim 1, it is characterised in that: the weight of the fermenting agent
Number is 3 parts, further includes 3-5 parts of brown sugar;The fermentation substrate includes 25 parts of the vinegar grain, 30 parts of the peanut shell, the apricot
15 parts of abalone mushroom mushroom bran, 0-10 part of calcium monohydrogen phosphate.
3. environmentally friendly pig raising fermenting bed padding as described in claim 1, which is characterized in that having in the fermenting agent
It imitates viable count to meet: the bacillus: the saccharomycete: the lactic acid bacteria: the actinomyces=2:1:2:1.
4. environmentally friendly pig raising fermenting bed padding as described in claim 1, it is characterised in that: the bacillus is selected from withered
Careless bacillus, bacillus licheniformis, bacillus coagulans, bacillus cereus, bacillus pumilus, one in clostridium butyricum
Kind is a variety of.
5. environmentally friendly pig raising fermenting bed padding as claimed in claim 4, it is characterised in that: the bacillus is by described
Bacillus subtilis and bacillus coagulans composition, and meet following living bacteria count ratio: the bacillus subtilis: institute
State bacillus coagulans=1:1.
6. the environmentally friendly pig raising preparation method of fermenting bed padding as described in any one of claim 1-5, which is characterized in that
The following steps are included:
The fermenting agent is added 20-30h of activation culture in brown sugar water with the amount of 5wt%, obtains activation fermentation by step (1)
Bacterium solution;
Step (2) weighs the material needed for the fermentation substrate, stirs and evenly mixs, obtains the fermentation substrate
The activation zymocyte liquid is inoculated with the fermentation substrate by step (3);
Step (4) adds water to adjust the fermentation substrate, makes the water content 55-65% of the fermentation substrate;
Step (5), heap fermentation 6-8 days.
7. the environmentally friendly pig raising preparation method of fermenting bed padding as claimed in claim 6, it is characterised in that: in the step
Suddenly in (2), the parts by weight of the cotton stalk and the calcium monohydrogen phosphate are controlled, make the carbon-nitrogen ratio C/N=of the fermentation substrate
25-40:1, carbon-phosphorus ratio C/P=60-80:1.
8. the environmentally friendly pig raising preparation method of fermenting bed padding as claimed in claim 7, it is characterised in that: in the step
Suddenly in (4), after the water content adjusting for completing the fermentation substrate, the pH value of the fermentation substrate is adjusted, its pH=7-9 is made.
9. the environmentally friendly pig raising preparation method of fermenting bed padding as claimed in claim 7, it is characterised in that: in the step
Suddenly in (4), the water content of the fermentation substrate is controlled 60% ± 1.5%.
10. the environmentally friendly pig raising preparation method of fermenting bed padding as claimed in claim 7, it is characterised in that: described
In step (5), when fermentation temperature rises to not less than 50 DEG C and continues 2 days, the material of the fermentation system is stirred
Continue to ferment after stirring.
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CN111919763A (en) * | 2020-08-04 | 2020-11-13 | 安徽科技学院 | Method for researching growth of live pigs on fermentation beds by using different fermentation bed bactericides |
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KR102719682B1 (en) | 2021-12-15 | 2024-10-21 | (주)옵트바이오 | Method for manufacturing functionalized wood particle for livestock barn flooring for accelerating decomposition and improving odor and method for manufacturing compost using functionalized wood particle |
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