CN112219937A

CN112219937A - Feed additive

Info

Publication number: CN112219937A
Application number: CN202011151522.3A
Authority: CN
Inventors: 汤海江; 汤先伟
Original assignee: Pizhou City Xiaohe Technology Development Co ltd; Jiangsu Xiaohe Biotechnology Research Institute Co ltd
Current assignee: Pizhou City Xiaohe Technology Development Co ltd; Jiangsu Xiaohe Biotechnology Research Institute Co ltd
Priority date: 2020-10-25
Filing date: 2020-10-25
Publication date: 2021-01-15

Abstract

The invention specifically discloses a feed additive, which is prepared from lactobacillus, bacillus, saccharomycetes and a grain material filler in a weight ratio of (2.5-5.0): 1.5-3.0: 1.5-2.5: 10-20 parts by weight; the filling material for the vinasse material is prepared from the following raw materials in parts by weight: purslane: hawthorn fruit: orange peel: herba seu radix Cirsii Japonici: herba Cirsii: seaweed: vinasse for brewing beer: beer =1:1:2:0.5:0.25:0.25:0.25:2:6, by cleaning, microwave drying, grinding, soaking in the vinasse solution, vacuum drying, pulverizing, and sieving. The feed additive disclosed by the invention not only has the efficacy of probiotics, but also enhances various nutritional and medicinal effects of products, and improves the application value of the products.

Description

Feed additive

Technical Field

The invention belongs to the technical field of feed processing, and particularly relates to a feed additive.

Background

In recent years, most of chemical drugs such as antibiotics are limited or forbidden in the breeding industry, disease prevention is achieved by utilizing the dominant flora of probiotics or enhancing nonspecific immunity, and the probiotics become the first choice in the breeding industry. The feed additive rich in probiotics is nontoxic to animal products, has no side effect on human bodies, and is the most vital green feed additive in the future breeding industry. However, most of the feed additives rich in probiotics currently supplied in the market are not considered from the perspective of nutritional value, and the selected excipients (also called fillers or diluents) are most stone powder, calcium carbonate, shell powder, wheat bran, rice hull powder, corn cob meal and the like, so that how to select and use raw materials with rich nutrition or functional health care as fillers and have the stability of live probiotics while considering the viable bacteria content of the probiotic feed additives is important for the research of containing the probiotic feed additives, and the improvement of the comprehensive efficacy and the stability in production and storage of the probiotic feed additives are the problems and development directions to be solved by the probiotic feed additives.

Disclosure of Invention

The invention aims to provide a novel feed additive, which consists of living probiotics and a grain material filling material, has the advantages of stable thalli and long survival time of the thalli, has better nutritional effect of the grain material filling material, has certain adhesion performance, and can be adsorbed on animal feed when the feed additive rich in the probiotics is added into the animal feed for mixing and stirring, so that the animals can take the feed conveniently, and the waste is reduced.

The invention relates to a feed additive, which is characterized in that the feed additive is prepared from lactobacillus, bacillus, saccharomycetes and a grain material filling material according to the weight ratio of 2.5-5.0: 1.5-3.0: 1.5-2.5: 10 to 20 parts by weight.

The lactobacillus is any one of enterococcus lactis, lactobacillus acidophilus, lactobacillus casei, lactobacillus delbrueckii subsp lactis (original name: lactobacillus lactis), lactobacillus plantarum, pediococcus acidilactici, pediococcus pentosaceus, streptococcus thermophilus, lactobacillus reuteri, lactobacillus cellobiosus, lactobacillus fermentum, lactobacillus delbrueckii subsp bulgaricus (original name: lactobacillus bulgaricus), lactobacillus buchneri and lactobacillus paracasei.

The bacillus is any one of bacillus licheniformis, bacillus subtilis, bacillus lentus, bacillus pumilus, bacillus coagulans and bacillus laterosporus (original name: bacillus laterosporus).

The yeast is any one of candida utilis, saccharomyces cerevisiae, feed yeast and rhodopseudomonas palustris.

The lactobacillus, the bacillus and the yeast are dry powder preparations of thallus containing viable bacteria, the dry powder preparations of the thallus can use all active thallus preparations which are sold in the market and used as feed additives, and the lactobacillus, the bacillus and the yeast can be respectively cultured in a liquid fermentation device by adopting a known method through an optimized culture medium, then the thallus in a fermentation liquid is separated by a high-speed centrifugation method, and then the bacterial sludge is dried by a hot air drying or vacuum freeze drying method to obtain the dry powder of the thallus with the viable bacteria concentration of 200 hundred million/g.

The preparation method of the feed additive is a conventional preparation method, and the feed additive product is prepared by premixing lactobacillus dry powder, bacillus dry powder and saccharomycete dry powder according to the proportion of the feed additive and then uniformly mixing the premixed lactobacillus dry powder, bacillus dry powder and saccharomycete dry powder with a grain material filling material.

The grain material is prepared from the following raw materials in parts by weight: purslane: hawthorn fruit: orange peel: herba seu radix Cirsii Japonici: herba Cirsii: seaweed: vinasse for brewing beer: beer =1:1:2:0.5:0.25:0.25:0.25:2:6, prepared by the following steps:

step (I): respectively selecting high-quality medicinal materials of dandelion, purslane, hawthorn, orange peel, Japanese thistle herb, common cephalanoplos herb and seaweed, cleaning, drying by microwave, and grinding into 10-mesh powder;

step (II): taking vinasse for brewing beer, and mixing the vinasse according to the proportion of 1:2, adding purified water according to the proportion, soaking for 1 day at the temperature of 30 ℃, and then filtering to obtain a soak solution;

step (three): mixing the soaking solution filtrate with beer to obtain a vinasse solution;

step (IV): putting the lees liquid obtained in the step (three) into a pickling jar or a barrel, adding the powder obtained in the step (one), stirring uniformly, and soaking for 2 weeks (14 days) at the temperature of 28 ℃;

step (V): vacuum drying the material after the grains are processed until the water content is less than or equal to 6 percent (by weight), crushing, and sieving by a 80-mesh sieve to prepare the filling material for the grains.

The invention has the advantages of overcoming the defects that the existence of probiotics contained in the beneficial microorganism bacterium preparation feed additive is singly highlighted, the nutritional value or the nutritional efficacy of the filling material is neglected, and the stability of the thallus of the viable bacterium preparation is neglected. The filling material for the vinasse material adopted by the invention is prepared from dandelion, purslane, hawthorn, orange peel, Japanese thistle herb, common cephalanoplos herb and seaweed by vinasse for brewing beer and brewing the beer.

The use method of the additive comprises the following steps: the piglet is added and used in a matching way according to 1.5-3.0% of the weight of the feed; the weight of the pigs below 50kg is added according to the proportion of 1-3% of the weight of the feed, and the weight of the pigs above 50kg is added according to the proportion of 1-2% of the weight of the feed. The poultry is added and proportioned according to 2-3% of the weight of the feed.

The herba Taraxaci is dried whole plant of Taraxacum mongolicum hand-Mazz, Taraxacum borealisinsense Kitam, or plants of the same genus. Dandelion is bitter, sweet and cold in nature. It enters liver and stomach meridians. Has the functions of clearing away heat and toxic material, eliminating swelling, dissipating stagnation, inducing diuresis and treating stranguria. The main treatment is as follows: sores and abscess, lung abscess, conjunctival congestion, pharyngalgia, damp-heat yellow gallbladder and heat stranguria.

Herba Portulacae is dry aerial part of Portulaca oleracea L. Purslane is sour and cold in flavor. It enters liver and large intestine meridians. Has the functions of clearing away heat and toxic material, cooling blood and stopping dysentery. The main treatment is as follows: diarrhea due to toxic heat, sore, yellow swelling, stranguria with heat, hematuria, hematochezia, snake and insect bite.

The fructus crataegi is dried mature fruit of Crataegus Pinnatafida Bge, var. major N.E.Br. or Crataegus Pinnatafida Bge. Haw is sour and sweet in nature and slightly warm in taste. It enters spleen, stomach and liver meridians. Has effects in promoting digestion, eliminating food stagnation, promoting qi circulation, and dispelling blood stasis. The main treatment is as follows: impairment of appetite, abdominal distension, dyspepsia, postpartum lochiorrhea, etc.

The pericarpium Citri Tangerinae is dried mature pericarp of Citrus reticulata Blanco of Rutaceae and its cultivar. Orange peel is bitter, pungent and warm in nature. It enters lung and spleen meridians. Has the functions of regulating qi, strengthening spleen, eliminating dampness and phlegm. The main treatment is as follows: appetite decrease, abdominal pain, abdominal distension, diarrhea, phlegm-dampness cough.

Cirsium japonicum is the dried aerial part of Cirsium japonicum Fisch. Circium japonicum is sweet, bitter and cool in nature. It enters heart and liver meridians. Has the functions of cooling blood, stopping bleeding, dissipating blood stasis and eliminating swelling. The main treatment is as follows: epistaxis, hematochezia, hematuria, metrorrhagia, traumatic hemorrhage, sore, yellow furuncle.

Herba Cephalanoploris is dry aerial part of Cirsium setosum (Willd.) MB. of Compositae. Herba Cephalanoploris is sweet, bitter and cool in nature. It enters heart and liver meridians. Has the functions of cooling blood, stopping bleeding, removing blood stasis and relieving swelling. It can be used for treating epistaxis, hematuria, carbuncle, swelling, sore, and traumatic hemorrhage.

The seaweed is dried algae of Sargassum Pallidum (Turn.) C.Ag. or Sargassum fusiforme (Harv.) Setch. Seaweed is bitter, salty and cold in nature. It enters liver, stomach and kidney meridians. Has the effects of softening and resolving hard mass, inducing diuresis and relieving swelling. Its composition contains carbohydrate 35-60%, protein 6-8%, mineral elements of iodine, potassium, calcium, magnesium, manganese, iron and zinc, etc. which are not comparable with terrestrial plant, and rich vitamins, alginic acid and mannitol, etc. The pharmacological action is as follows: has anticoagulant, blood lipid reducing, blood pressure lowering, microcirculation improving, antiulcer, antitumor, and antiinfectious effects.

The "%" in the present invention is a weight percentage.

The invention has the advantages of

The lees material filling material contains Chinese herbal medicine components with the functions of clearing away heat and toxic materials, cooling blood and stopping dysentery, helping digestion and removing stasis, regulating qi and strengthening spleen, dissipating blood stasis and reducing swelling, cooling blood and stopping bleeding, inducing diuresis and reducing swelling and the like, so that the product not only embodies the efficacy of beneficial bacteria, but also enhances various nutrition and medication effects of the product, and improves the application value of the product.

The purslane, hawthorn and seaweed components in the grain material contain rich mucilage and colloidal substances, and form a layer adhesive film on the surface of the feed, so that the adhesive force of the additive and the feed is increased, and the utilization rate of the additive is improved; the animal immunity can be improved and the quality of meat and eggs can be improved by the rich nutrient components such as polysaccharide, mineral elements, iodine, mannitol, vitamins and the like and various bioactive substances in the Chinese herbal medicines; the vinasse material is rich in macroelements, microelements, vitamins, amino acids and other rich nutrient substances, can improve the nutrient medium of probiotics, promote the metabolism of animals, improve the disease resistance of organisms and promote the growth of the animals, thereby improving the economic benefit; the Chinese herbal medicine components in the vinasse material, such as hawthorn, seaweed and the like, contain acidic substances, so that the digestion function of animals can be improved, and an acidic environment of animal intestinal tracts is created for probiotics, so that the functional effect of the probiotics is facilitated; the filling material of the vinasse material is rich in nutrient substances, and the survival rate of the mixed product of the filling material and probiotics in the closed package of thalli can be obviously improved.

When in use, the feed additive and the feed are fully mixed, a small amount of water can be properly added, and the mixture is uniformly stirred, so that the contained probiotics are adhered to the surface of the feed, the waste of living bacteria of animals is reduced in the feeding process, and the utilization rate of the probiotics can be improved.

The product of the invention theoretically can improve the nutritive value of the microorganism bacteria, promote the metabolism of animals, improve the digestive function, improve the disease resistance of organisms and promote the growth of the animals. In order to research the using effect of the product, the product is used for testing and researching the influence of the product on the growth of piglets and broilers.

Respectively selecting 150 healthy three-element hybrid piglets with Duda length, 3000 broilers and 3000 laying hens in a pig farm of Yanghu Zhenghuacun and a Yang XX poultry farm of Chenolou Zhenghuacun in China, wherein the three-element hybrid piglets are divided into three groups: tests 1 (antibiotic group), 2 (blank group) and 3 (additive product of the invention is added), wherein each group is set to 5 times for repeating, the tests are carried out, the complete compound feed used in the tests 1-3 is produced by Xiao river science and technology development Limited company in the city of Bizhou, and the antibiotic group 1 is prepared by antibiotic-added complete feed; 2 groups of blank groups are prepared by complete feed without antibiotics; 3 groups of the additive product of the invention are added with 2-3 percent.

The pig test results are as follows: daily gain/g: trial 1 group 631.2; trial 2 group 604.1; run 3 group 656.1. From the test results, the piglets in the 3 groups have the largest weight gain, the piglets in the 1 group have the second weight gain, the piglets in the 2 groups have the lowest weight gain, and the differences between the 3 groups and the 2 groups are obvious through the difference significance test.

The feed intake of the test 3 group was increased by 5.3% and 5.5% compared to the test 1 group and the test 2 group, respectively.

Combining the above experimental results, the weight gain and feed intake of the test 3 groups were higher than those of the other two groups.

2.5-3% of the additive product is added into the complete feed for chickens, the daily gain is improved by 2.6-17.2% compared with a test 1 group (antibiotic group), is improved by 3.6-21.7% compared with a test 2 group (blank group), the feed is saved by 6.3-10.5%, the laying rate is improved by 2.2-6.8%, and the test result shows that the additive product is superior to the antibiotic group. Through the observation of the test process, the livestock and poultry have normal performance, smooth and bright fur, normal appetite and active and normal activity, and the pathology examination test group is basically the same as the blank group, so that the additive product of the invention has no adverse effect on the mechanism of the livestock and poultry.

Detailed Description

The present invention is further illustrated by the following examples, which should not be construed as limiting the invention.

Example 1

Respectively culturing enterococcus lactis, lactobacillus acidophilus, lactobacillus casei, lactobacillus lactis, lactobacillus plantarum, pediococcus lactis, pediococcus pentosaceus, streptococcus thermophilus, lactobacillus reuteri, lactobacillus cellobiosus, lactobacillus fermentum, lactobacillus bulgaricus, lactobacillus buchneri and lactobacillus paracasei by using corresponding optimized culture medium through liquid fermentation equipment, separating thalli in fermentation liquor by using a high-speed centrifuge, drying the centrifuged bacterial sludge in a hot air or vacuum freeze drying mode to obtain enterococcus lactis dry powder with 200 hundred million/g of viable bacteria, lactobacillus acidophilus dry powder with 200 hundred million/g of viable bacteria, lactobacillus casei dry powder with 200 hundred million/g of viable bacteria, lactobacillus lactis dry powder with 200 hundred million/g of viable bacteria, lactobacillus plantarum dry powder with 200 hundred million/g of viable bacteria, lactobacillus casei dry powder with 200 million/g of viable bacteria, lactobacillus paracasei, The main raw materials of the lactobacillus and the lactobacillus paracasei powder comprise pediococcus acidilactici dry powder with 200 hundred million/g of viable bacteria, streptococcus thermophilus dry powder with 200 hundred million/g of viable bacteria, lactobacillus reuteri dry powder with 200 hundred million/g of viable bacteria, cellobiose lactobacillus powder with 200 hundred million/g of viable bacteria, lactobacillus fermentum dry powder with 200 hundred million/g of viable bacteria, lactobacillus bulgaricus dry powder with 200 hundred million/g of viable bacteria, lactobacillus buchneri dry powder with 200 hundred million/g of viable bacteria and lactobacillus paracasei dry powder with 200 hundred million/g of viable bacteria.

Example 2

Respectively culturing bacillus licheniformis, bacillus subtilis, bacillus lentus, bacillus pumilus, bacillus coagulans and bacillus laterosporus by using a corresponding optimized culture medium through liquid fermentation equipment, separating thalli in fermentation liquor by using a high-speed centrifuge, and drying the centrifuged bacterial sludge in a hot air or vacuum freeze drying mode to obtain 200 hundred million/g of bacillus licheniformis dry powder, 200 hundred million/g of bacillus subtilis dry powder, 200 hundred million/g of bacillus lentus dry powder, 200 hundred million/g of bacillus pumilus dry powder, 200 hundred million/g of bacillus coagulans dry powder and 200 hundred million/g of bacillus laterosporus dry powder.

Example 3

The method comprises the steps of using corresponding optimized culture medium, respectively culturing Candida utilis, saccharomyces cerevisiae, feed yeast and rhodopseudomonas palustris by liquid fermentation equipment, then separating out thalli in fermentation liquor by using a high-speed centrifuge, and drying the centrifuged bacterial sludge in a hot air or vacuum freeze drying mode to obtain dried Candida utilis powder with the viable bacteria content of 200 hundred million/g, dried saccharomyces cerevisiae powder with the viable bacteria content of 200 hundred million/g, dried feed yeast powder with the viable bacteria content of 200 hundred million/g and dried rhodopseudomonas palustris powder with the viable bacteria content of 200 hundred million/g.

Example 4

Preparation of the vinasse filling material:

step (I): respectively selecting high-quality medicinal materials of 1 kg of dandelion, 1 kg of purslane, 2 kg of hawthorn, 0.5 kg of orange peel, 0.25 kg of Japanese thistle herb, 0.25 kg of common cephalanoplos herb and 0.25 kg of seaweed, cleaning, drying by microwave, and crushing into 10-mesh powder;

step (II): taking 2 kg of vinasse for brewing beer, adding 4 kg of purified water, soaking for 1 day at 30 ℃, and then squeezing and filtering to obtain a soaking solution;

step (three): adding 6 kilograms of beer into the soak solution obtained in the step (II) and mixing to obtain a vinasse solution;

step (IV): putting the vinasse liquid obtained in the step (three) into a pickling tank or a barrel, adding the powder obtained in the step (one), uniformly stirring, and soaking for 14 days at the temperature of 28 ℃;

step (V): and (3) placing the material after being pickled into a vacuum drier for vacuum drying to ensure that the moisture content of the material is less than or equal to 6 percent, crushing the dried material, and sieving the crushed material with a 80-mesh sieve to prepare the filling material for the pickled material.

Example 5

Weighing 250g of enterococcus lactis dry powder obtained in example 1, 150g of bacillus licheniformis dry powder obtained in example 2 and 150g of candida utilis dry powder obtained in example 3, mixing the powders into uniform thallus dry powder, weighing 1000g of the grain material filling material obtained in example 4, mixing the mixed thallus dry powder and the grain material filling material, putting the mixture into a stirrer, fully stirring and uniformly mixing, and sealing and packaging to obtain the feed additive product.

The mass ratio of the thalli enterococcus lactate dry powder, the bacillus licheniformis dry powder and the candida utilis dry powder to the filler in the product of the embodiment is 2.5: 1.5: 1.5: 10, after the sample retention and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thalli and the control group thalli are respectively 83 percent and 61 percent of the lactic acid enterococcus; 86% and 70% of bacillus licheniformis and 92% and 89% of candida utilis. The control group was identical to the product of the example except that all the fillers were bran.

Example 6

Weighing 500g of enterococcus lactis dry powder obtained in example 1, 300g of bacillus licheniformis dry powder obtained in example 2 and 250g of candida utilis dry powder obtained in example 3, mixing the powder into uniform thallus dry powder, weighing 1000g of the distiller's grains filling material obtained in example 4, mixing the mixed thallus dry powder and the distiller's grains filling material, putting the mixture into a stirrer, fully stirring and uniformly mixing, and sealing and packaging to obtain the feed additive product.

The mass ratio of the thalli enterococcus lactate dry powder, the bacillus licheniformis dry powder and the candida utilis dry powder to the filler in the product of the embodiment is 5: 3: 2.5: 10, after the sample retention and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thalli and the control group thalli are 93 percent and 64 percent respectively for the enterococcus lactis; 89% and 68% of bacillus licheniformis and 95% and 90% of candida utilis. The control group was identical to the product of the example except that all the fillers were bran.

Example 7

Weighing 500g of lactobacillus acidophilus dry powder obtained in example 1, 300g of bacillus subtilis dry powder obtained in example 2 and 250g of saccharomyces cerevisiae dry powder obtained in example 3, mixing the powder into uniform thallus dry powder, weighing 2000g of the spent grain material filling material obtained in example 4, mixing the mixed thallus dry powder and the spent grain material filling material, putting the mixture into a stirrer, fully stirring and uniformly mixing the mixture, and sealing and packaging the mixture to obtain the feed additive product.

The mass ratio of the thallus lactobacillus acidophilus dry powder, the bacillus subtilis dry powder and the saccharomyces cerevisiae dry powder to the filling material in the product of the embodiment is 5: 3: 2.5: 20, after the sample retention and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thalli and the control group thalli are respectively 89 percent and 63 percent of lactobacillus acidophilus; 89% and 72% of bacillus subtilis and 96% and 91% of saccharomyces cerevisiae. The control group was identical to the product of the example except that all the fillers were bran.

Example 8

Weighing 250g of the pediococcus acidilactici dry powder obtained in example 1, 150g of the bacillus lentus dry powder obtained in example 2 and 250g of the feed yeast dry powder obtained in example 3, mixing the dry powder into uniform thallus dry powder, weighing 1000g of the spent grain material filling material obtained in example 4, mixing the mixed thallus dry powder and the spent grain material filling material, putting the mixture into a stirrer, fully stirring and uniformly mixing the mixture, and sealing and packaging the mixture to obtain the feed additive product.

The mass ratio of the thallus pediococcus acidilactici dry powder, the bacillus lentus dry powder, the feed yeast dry powder and the filler in the product of the embodiment is 2.5: 1.5: 2.5: 10, after the retained sample and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thallus and the control group thallus are respectively 90 percent and 68 percent of pediococcus acidilactici; 89% and 72% of Bacillus lentus and 94% and 90% of feed yeast. The control group was identical to the product of the example except that all the fillers were bran.

Example 9

Weighing 500g of lactobacillus plantarum dry powder obtained in example 1, 150g of bacillus pumilus dry powder obtained in example 2 and 250g of saccharomyces cerevisiae dry powder obtained in example 3, mixing the powders into uniform thallus dry powder, weighing 1000g of the spent grain material filling material obtained in example 4, mixing the mixed thallus dry powder and the spent grain material filling material, putting the mixture into a stirrer, fully stirring and uniformly mixing the mixture, and sealing and packaging the mixture to obtain the feed additive product.

The mass ratio of the thallus lactobacillus plantarum dry powder, the bacillus pumilus dry powder and the saccharomyces cerevisiae dry powder to the filling material in the product of the embodiment is 5: 1.5: 2.5: 10, after the retained sample and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thalli and the control group thalli are respectively 86 percent and 62 percent of the lactobacillus plantarum; 89% and 68% of Bacillus pumilus and 94% and 90% of Saccharomyces cerevisiae. The control group was identical to the product of the example except that all the fillers were bran.

Example 10

375g of streptococcus thermophilus dry powder obtained in the embodiment 1, 225g of bacillus subtilis dry powder obtained in the embodiment 2 and 200g of candida utilis dry powder obtained in the embodiment 3 are weighed and mixed into uniform thallus dry powder, 1000g of the lees material filling material obtained in the embodiment 4 is weighed and mixed, the mixed thallus dry powder and the lees material filling material are put into a stirrer to be fully stirred and uniformly mixed, and then the mixture is sealed and packaged to obtain the feed additive product.

The mass ratio of the thallus streptococcus thermophilus dry powder, the bacillus subtilis dry powder and the candida utilis dry powder to the filler in the product of the embodiment is 3.75: 2.25: 2: 10, after the retained sample and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thalli and the control group thalli are respectively 85 percent and 60 percent of streptococcus thermophilus; 88% and 70% of bacillus subtilis and 91% and 88% of candida utilis. The control group was identical to the product of the example except that all the fillers were bran.

Example 11

Weighing 300g of the lactobacillus fermentum dry powder obtained in the embodiment 1, 300g of the bacillus licheniformis dry powder obtained in the embodiment 2 and 200g of the saccharomyces cerevisiae dry powder obtained in the embodiment 3, mixing the powder into uniform thallus dry powder, weighing 1000g of the spent grain material filling material obtained in the embodiment 4, mixing the mixed thallus dry powder and the spent grain material filling material, putting the mixture into a stirrer, fully stirring and uniformly mixing the mixture, and sealing and packaging the mixture to obtain the feed additive product.

The mass ratio of the thalli lactobacillus fermentum dry powder, the bacillus licheniformis dry powder, the saccharomyces cerevisiae dry powder and the filling material in the product of the embodiment is 3: 3: 2: 10, after the preserved sample and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thalli and the control group thalli are respectively 84 percent and 60 percent of lactobacillus fermentum; 85% and 68% of bacillus licheniformis and 94% and 90% of saccharomyces cerevisiae. The control group was identical to the product of the example except that all the fillers were bran.

Example 12

Weighing 500g of the lactobacillus bulgaricus dry powder obtained in the embodiment 1, 300g of the bacillus subtilis dry powder obtained in the embodiment 2 and 250g of the rhodopseudomonas palustris dry powder obtained in the embodiment 3, mixing the powders into uniform thallus dry powder, weighing 2000g of the lees material filling material obtained in the embodiment 4, mixing the mixed thallus dry powder and the lees material filling material, putting the mixture into a stirrer, fully stirring and uniformly mixing the mixture, and sealing and packaging the mixture to obtain the feed additive product.

The mass ratio of the thallus lactobacillus bulgaricus dry powder, the bacillus subtilis dry powder, the rhodopseudomonas palustris dry powder to the filler in the product of the embodiment is 5: 3: 2.5: 20, after the preserved sample and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thalli and the control group thalli are 82 percent and 60 percent of the lactobacillus bulgaricus respectively; 87% and 68% of bacillus subtilis and 89% and 78% of rhodopseudomonas palustris. The control group was identical to the product of the example except that all the fillers were bran.

Example 13

Weighing 300g of lactobacillus reuteri dry powder obtained in example 1, 300g of bacillus licheniformis dry powder obtained in example 2 and 250g of feed yeast dry powder obtained in example 3, mixing the powder into uniform thallus dry powder, weighing 1500g of the spent grain material filling material obtained in example 4, mixing the mixed thallus dry powder and the spent grain material filling material, putting the mixture into a stirrer, fully stirring and uniformly mixing the mixture, and sealing and packaging the mixture to obtain the feed additive product.

The mass ratio of the lactobacillus reuteri dry powder, the bacillus licheniformis dry powder, the feed yeast dry powder and the filling material in the product of the embodiment is 3: 3: 2.5: 15, after the preserved sample and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thalli and the control group thalli are respectively 86 percent and 68 percent of the lactobacillus reuteri; 88% and 71% of Bacillus licheniformis and 93% and 78% of feed yeast. The control group was identical to the product of the example except that all the fillers were bran.

Example 14

Weighing 300g of the pediococcus acidilactici dry powder obtained in example 1, 200g of the bacillus coagulans dry powder obtained in example 2 and 200g of the feed yeast dry powder obtained in example 3, mixing the powder into uniform thallus dry powder, weighing 1300g of the spent grain material filling material obtained in example 4, mixing the mixed thallus dry powder and the spent grain material filling material, putting the mixture into a stirrer, fully stirring and uniformly mixing the mixture, and sealing and packaging the mixture to obtain the feed additive product.

The mass ratio of the thallus pediococcus acidilactici dry powder, the bacillus coagulans dry powder, the feed mother dry powder and the filling material in the product of the embodiment is 3: 2: 2: 13, after the retained sample and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thallus and the control group thallus are 82 percent and 63 percent of pediococcus acidilactici respectively; the bacillus coagulans accounts for 83% and 66%, and the feed yeast accounts for 90% and 85%. The control group was identical to the product of the example except that all the fillers were bran.

Example 15

500g of the cellobiose lactobacillus dry powder obtained in the embodiment 1, 300g of the bacillus subtilis dry powder obtained in the embodiment 2 and 200g of the candida utilis dry powder obtained in the embodiment 3 are weighed and mixed to form uniform thallus dry powder, 1000g of the grain material filling material obtained in the embodiment 4 is weighed and mixed, the mixed thallus dry powder and the grain material filling material are placed in a stirrer to be fully stirred and uniformly mixed, and then the mixture is sealed and packaged to obtain the feed additive product.

The mass ratio of the thalli lactobacillus cellobiose dry powder, the bacillus subtilis dry powder and the candida utilis dry powder to the filler in the product of the embodiment is 5: 3: 2: 10, after the retained sample and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thalli and the control group thalli are 84 percent and 62 percent respectively for the lactobacillus cellobiose; 88% and 67% of bacillus subtilis and 91% and 86% of candida utilis. The control group was identical to the product of the example except that all the fillers were bran.

Example 16

Weighing 500g of lactobacillus casei dry powder obtained in example 1, 250g of bacillus pumilus dry powder obtained in example 2 and 250g of feed yeast dry powder obtained in example 3, mixing the powder into uniform thallus dry powder, weighing 2000g of the spent grain material filling material obtained in example 4, mixing the mixed thallus dry powder and the spent grain material filling material, putting the mixture into a stirrer, fully stirring and uniformly mixing the mixture, and sealing and packaging the mixture to obtain the feed additive product.

The mass ratio of the lactobacillus casei dry powder, the bacillus pumilus dry powder, the feed yeast dry powder and the filling material in the product of the embodiment is 5: 2.5: 2.5: 20, after the retained sample and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thalli and the control group thalli are respectively 89 percent and 72 percent of lactobacillus casei; 88% and 67% of Bacillus pumilus and 93% and 87% of feed yeast. The control group was identical to the product of the example except that all the fillers were bran.

Example 17

Weighing 450g of lactobacillus lactis dry powder obtained in example 1, 300g of bacillus coagulans dry powder obtained in example 2 and 200g of saccharomyces cerevisiae dry powder obtained in example 3, mixing the powder into uniform thallus dry powder, weighing 1800g of the spent grain material filling material obtained in example 4, mixing the mixed thallus dry powder and the spent grain material filling material, putting the mixture into a stirrer, fully stirring and uniformly mixing, and sealing and packaging to obtain the feed additive product.

The mass ratio of the thallus lactobacillus lactis dry powder, the bacillus coagulans dry powder and the saccharomyces cerevisiae dry powder to the filler in the product of the embodiment is 4.5: 3: 2: 18, after the sample retention and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thalli and the control group thalli are respectively 85 percent and 63 percent of the lactobacillus lactis; the bacillus coagulans is 87% and 69%, and the saccharomyces cerevisiae is 94% and 87%. The control group was identical to the product of the example except that all the fillers were bran.

Example 18

Weighing 400g of the lactobacillus fermentum dry powder obtained in the embodiment 1, 200g of the bacillus pumilus dry powder obtained in the embodiment 2 and 200g of the rhodopseudomonas palustris dry powder obtained in the embodiment 3, mixing the powder into uniform thallus dry powder, weighing 1200g of the spent grain material filling material obtained in the embodiment 4, mixing the mixed thallus dry powder and the spent grain material filling material, putting the mixture into a stirrer, fully stirring and uniformly mixing the mixture, and sealing and packaging the mixture to obtain the feed additive product.

The mass ratio of the thalli lactobacillus fermentum dry powder, the bacillus pumilus dry powder, the rhodopseudomonas palustris dry powder and the filler in the product of the embodiment is 4: 2: 2: 12, after the sample retention and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thalli and the control group thalli are respectively 85 percent and 62 percent of that of the lactobacillus fermentum; 87% and 66% of bacillus pumilus and 90% and 84% of rhodopseudomonas palustris. The control group was identical to the product of the example except that all the fillers were bran.

Example 19

Weighing 450g of lactobacillus lactis dry powder obtained in example 1, 250g of bacillus licheniformis dry powder obtained in example 2 and 200g of candida utilis obtained in example 3, mixing the powder into uniform thallus dry powder, weighing 1100g of the spent grain material filling material obtained in example 4, mixing the mixed thallus dry powder and the spent grain material filling material, putting the mixture into a stirrer, fully stirring and uniformly mixing, and sealing and packaging to obtain the feed additive product.

The mass ratio of the thallus lactobacillus lactis dry powder, the bacillus licheniformis dry powder and the candida utilis dry powder to the filler in the product of the embodiment is 4.5: 2.5: 2: 11, after the retained sample and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thalli and the control group thalli are respectively 85 percent and 62 percent of lactobacillus lactis; 88% and 71% of bacillus licheniformis and 93% and 88% of candida utilis. The control group was identical to the product of the example except that all the fillers were bran.

Example 20

Weighing 500g of lactobacillus fermentum dry powder obtained in example 1, 300g of bacillus subtilis dry powder obtained in example 2 and 250g of feed yeast dry powder obtained in example 3, mixing the two powders into uniform thallus dry powder, weighing 1500g of the spent grain material filling material obtained in example 4, mixing the mixed thallus dry powder and the spent grain material filling material, putting the mixture into a stirrer, fully stirring and uniformly mixing the mixture, and sealing and packaging the mixture to obtain the feed additive product.

The mass ratio of the lactobacillus fermentum dry powder, the bacillus subtilis dry powder, the feed yeast dry powder and the filler in the product of the embodiment is 5: 3: 2.5: 15, after the retained sample and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thalli and the control group thalli are respectively 86 percent and 61 percent of lactobacillus fermentum; 84 percent and 65 percent of bacillus subtilis and 96 percent and 87 percent of feed yeast. The control group was identical to the product of the example except that all the fillers were bran.

Example 21

Weighing 250g of the lactobacillus thermophilus dry powder obtained in the embodiment 1, 250g of the bacillus coagulans dry powder obtained in the embodiment 2 and 250g of the candida utilis dry powder obtained in the embodiment 3, mixing the powders into uniform thallus dry powder, weighing 1000g of the lees material filling material obtained in the embodiment 4, mixing the mixed thallus dry powder and the lees material filling material, putting the mixture into a stirrer, fully stirring and uniformly mixing the mixture, and sealing and packaging the mixture to obtain the feed additive product.

The mass ratio of the thalli lactobacillus thermophilus dry powder, the bacillus coagulans dry powder and the candida utilis dry powder to the filler in the product of the embodiment is 2.5: 2.5: 2.5: 10, after the retained sample and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thalli and the control group thalli are respectively 86 percent and 60 percent of lactobacillus thermophilus; 85% and 67% of bacillus coagulans and 94% and 88% of candida utilis. The control group was identical to the product of the example except that all the fillers were bran.

Example 22

Weighing 500g of lactobacillus lactis dry powder obtained in example 1, 150g of bacillus pumilus dry powder obtained in example 2 and 150g of saccharomyces cerevisiae dry powder obtained in example 3, mixing the powder into uniform thallus dry powder, weighing 1000g of the spent grain material filling material obtained in example 4, mixing the mixed thallus dry powder and the spent grain material filling material, putting the mixture into a stirrer, fully stirring and uniformly mixing the mixture, and sealing and packaging the mixture to obtain the feed additive product.

The mass ratio of the thallus lactobacillus lactis dry powder, the bacillus pumilus dry powder and the saccharomyces cerevisiae dry powder to the filler in the product of the embodiment is 5: 1.5: 1.5: 10, after the preserved sample and the control group are stored for 12 months at the temperature of 25 ℃, the survival rates of the product thalli and the control group thalli are respectively 84 percent and 61 percent of lactobacillus lactis; 87% and 66% of Bacillus pumilus and 95% and 89% of Saccharomyces cerevisiae. The control group was identical to the product of the example except that all the fillers were bran.

Claims

1. A feed additive is characterized by comprising lactobacillus, bacillus, saccharomycetes and a grain material filling material in a weight ratio of 2.5-5.0: 1.5-3.0: 1.5-2.5: 10-20 parts by weight; the filling material for the vinasse material is prepared from the following raw materials in parts by weight: purslane: hawthorn fruit: orange peel: herba seu radix Cirsii Japonici: herba Cirsii: seaweed: vinasse for brewing beer: beer =1:1:2:0.5:0.25:0.25:0.25:2:6, prepared by the following steps:

step (IV): putting the lees liquid obtained in the step (three) into a pickling jar or a barrel, adding the powder obtained in the step (one), stirring uniformly, and soaking at 28 ℃ for 2 weeks;

step (V): vacuum drying the material after the grains are processed until the water content is less than or equal to 6%, crushing, and sieving with a 80-mesh sieve to obtain the filling material for the grains.

2. The feed additive of claim 1, wherein: the lactobacillus is any one of enterococcus lactis, lactobacillus acidophilus, lactobacillus casei, lactobacillus delbrueckii subsp lactis, lactobacillus plantarum, pediococcus acidilactici, pediococcus pentosaceus, streptococcus thermophilus, lactobacillus reuteri, lactobacillus cellobiosus, lactobacillus fermentum, lactobacillus delbrueckii subsp bulgaricus, lactobacillus buchneri and lactobacillus paracasei.

3. The feed additive of claim 1, wherein: the bacillus is any one of bacillus licheniformis, bacillus subtilis, bacillus lentus, bacillus pumilus, bacillus coagulans and bacillus laterosporus.

4. The feed additive of claim 1, wherein: the yeast is any one of candida utilis, saccharomyces cerevisiae, feed yeast and rhodopseudomonas palustris.

5. The feed additive according to claim 2, 3 or 4, wherein: the lactobacillus, the bacillus and the microzyme are dry powder preparations of which each gram contains 200 hundred million viable bacteria.