CN103919816A - Composite probiotic spray for preventing and treating newborn livestock diarrhea and preparation method of spray - Google Patents
Composite probiotic spray for preventing and treating newborn livestock diarrhea and preparation method of spray Download PDFInfo
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- CN103919816A CN103919816A CN201410176927.0A CN201410176927A CN103919816A CN 103919816 A CN103919816 A CN 103919816A CN 201410176927 A CN201410176927 A CN 201410176927A CN 103919816 A CN103919816 A CN 103919816A
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Abstract
The invention discloses a composite probiotic spray for preventing and treating newborn livestock diarrhea, and discloses a preparation method of the spray. The composite probiotic spray for preventing and treating newborn livestock diarrhea is prepared from the following raw materials in parts by weight: 200-1,000 parts of adsorptive support, 400-2,000 parts of coated support and 100-200 parts of probiotic composition. The preparation has the effects of regulating intestinal micro-ecological balance of newborn young livestock, keeping normal physiological functions of intestines, enhancing immunity of organisms, improving the disease resistance of organisms, and in particular effectively preventing physiological and pathological diarrhea of young livestock. The composite probiotic spray is unique in dosage form, convenient and efficient to apply, more suitable for newborn young livestock, and is suitably popularized and applied in animal husbandry production.
Description
Technical field
The invention belongs to veterinary drug technical field, be specifically related to a kind of compound probiotic spray of preventing and treating newborn diarrhea in livestock and preparation method thereof.
Background technology
Newborn domestic animal (comprising pig, cattle, sheep, horse etc.) diarrhoeal diseases is one of difficult problem of puzzlement aquaculture always, is also the principal disease that endangers at present aquaculture.According to statistics; pig farm and sheep raising field in scale; the sickness rate of newborn piglet and lamb diarrhoea disease is up to 75% and 62%; the generation of diarrhoeal diseases not only causes that young stock mortality rate increases, survival rate declines; and the reduction of young stock growth performance, feed conversion rate are declined; increased aquaculture cost, reduced culture benefit, the economic loss of bringing thus every year can billions of units.The reason that causes newborn young stock diarrhoea is many-sided, the first, and pathogenicity factor, comprises that the pathogenic infections such as antibacterial, virus, parasite cause; The second, irritability factor: comprise the change of environment, extraneous stimulation etc.The 3rd, Physiological factors, because young stock lacks innate immunity power and digestive function growth imperfection.Wherein the factor of body self accounts for leading, because immune system and the digestive system of newborn young stock are not grown completely, especially the normal flora fauna in digestive tract is not set up completely, resistance is to external world very weak, young stock is contacting or is eating with the external world in newborn process, and contact pathogenic microorganism causes diarrhoea.This diarrhoea period of disease is for birth was to latter 1 month of wean.At present, in aquaculture, to the control of newborn young stock diarrhoea, be mainly vaccine prevention and antibiotic treatment, because vaccine immunity is specific immunity, in addition cause of disease easily morphs, therefore the effect of vaccine prevention is limited, and cost is higher, in pig industry, other aquaculture is not is not prevented and treated this disease with vaccine; Present stage, in aquaculture, very general with antibiotic prophylaxis and treatment young stock diarrhoea, but along with antibiotic extensive use, the series of problems of drawing thus causes people's great attention, especially young stock is used the growth of antibiotic meeting Immunosuppression organ for a long time or in a large number, destroys the organa parenchymatosums such as liver, kidney, causes immunosuppressant.In addition, antibiotic use can have no the profitable strain that kills of selecting, and causes alteration of intestinal flora, thereby brings out the generation of diarrhoea and Other diseases.In recent years, research is found to use antibiotic can bring out antibiotic-associated diarrhea at young animal, therefore countries in the world are taked compulsory measure one after another, the aspects such as feeding antibiotic kind, using method, dosage are strictly limited, and some American-European countries prohibite to add in animal cultivation and feedstuff and use antibiotic.Therefore, in aquaculture production, be badly in need of a kind of preparation efficient, that have no side effect and prevent and treat white scours.
Probiotics preparation is a quasi-microorganism active bacteria formulation, it has the field planting and breeding, the enhancing human body immunity function that maintain intestinal microecology balance, suppress pathogen, produces useful metabolite and reduce the effects such as harmful substance generation such as amine, indole, now by people, is familiar with gradually and becomes one of antibiotic substitute products.In recent years, having developed many probiotics preparations both at home and abroad, is main single or compound formulation mainly with lactobacillus, bacillus bifidus and bacillus cereus etc.Dissimilar preparation is different for the effect of the animal generation of different physiological stages, as some preparation has good effect to young animal, but to adults poor effect.We have looked into newly Chinese patent and pertinent literature about probiotics preparation aspect at present, find that the large form mainly with feed additive occurs, and newborn young stock can't eat feedstuff, therefore such probiotics preparation is restricted in newborn young stock application.
Summary of the invention
The object of this invention is to provide a kind of compound probiotic spray of preventing and treating newborn diarrhea in livestock, to overcome the deficiency of existing product, being more suitable for newborn domestic animal uses, the present invention can make profitable strain field planting as early as possible in young stock intestinal, reach the balance of intestinal microecology, thus the generation of prevention and treatment white scours.
In order to realize above object, a kind of compound probiotic spray provided by the invention is that the raw material by following weight portion is prepared from: absorption carrier 200-1000 part, coated carrier 400-2000 part, probiotic bacteria 100-200 part.
Wherein, described probiotic bacteria comprises Bacillus subtillis, Bacillus licheniformis, Bacillus cereus, lactobacillus, bacillus bifidus, enterococcus faecalis, Lactobacillus plantarum, yeast, the compositions of at least three kinds in streptococcus thermophilus.To achieve the object of the present invention, strain used is pure culture, and the viable count of cultivation bacterium liquid is not less than 2 * 10
8cFU/ml.
Absorption carrier of the present invention be in defatted milk powder, soybean protein powder, trehalose, sodium lactate, cysteine any, two or more.
Coated carrier of the present invention be in sucrose, glucose, soluble starch, dextrin, essence, mannose, zeolite powder, sodium alginate any, two or more.
The present invention also aims to provide a kind of preparation method of preventing and treating the compound probiotic spray of newborn diarrhea in livestock.
The preparation method of the compound probiotic spray of the newborn diarrhea in livestock of control of the present invention comprises:
1) preparation of fermentation liquid: by Bacillus subtillis, Bacillus licheniformis, Bacillus cereus, lactobacillus, bacillus bifidus, enterococcus faecalis, Lactobacillus plantarum, yeast, the probiotic bacterias such as streptococcus thermophilus are inoculated into respectively separately in optimum medium, cultivate under suitable condition and within 18-48 hour, reach its exponential phase, then being transferred in fermentation tank and cultivating in a large number, is that in fermentation liquid, viable bacteria concentration reaches 2 * 10 when reaching exponential phase
8when CFU/ml is above, stop cultivating.
2) preparation of thalline: getting fermentation liquid and carry out high speed centrifugation, is 3000～6000 revs/min by rotating speed, collects thalline in centrifugal 15～30 minutes standby.
3) by absorption carrier 110 ℃ of autoclavings 15 minutes, cooling stand-by; Press certain weight ratio by step 2) the different thalline of gained mix, and get its 100～200 parts and 200～1000 parts of mixing of absorption carrier, and the speed by 50 revs/min in the environment lower than 20 ℃ stirs 30～60 minutes, and thalline is thoroughly adsorbed onto on carrier.
4) get 400～2000 parts, coated carrier and step 3) gained mixture mixes by a certain percentage, and by step 3) method stir 30 minutes, (volume ratio 1: 10～20) sprays into 2%～8% sucrose solution in mixture more in proportion, continue to stir 30 minutes, room temperature aeration-drying, packs and get final product.
The applying step of spray of the present invention is that the ratio of spray of the present invention 1: 50 in mass ratio～200 and water mixing are shaken up, and packs in aerosol apparatus, is directly sprayed onto breast portion and the body surface of female animal when young stock is born rear and suckling.Also the present invention can be sprayed on to feedstuff surface, for young stock free choice feeding.
The invention has the advantages that: 1, the preferred probiotic bacteria of the present invention is nonpathogenic probiotics, can field planting arrive in the intestinal of newborn young stock, and breed in a large number, by dominant microflora, biological antagonist, effects such as metabolite that generation is useful, improve intestine microenvironment, maintain the normal physiological function of intestinal.Thereby can effectively prevent newborn young stock physiological and pathologic diarrhoea.2, preparation technology of the present invention selects double-deck coating technique, can effectively protect probiotic bacteria to enter young stock gastric and by gastric acid and protease, not destroyed, and then play a role in arrival intestinal; This technique can extend the pot-life of probiotic active simultaneously, overcomes existing short shortcoming of probiotic products pot-life.3, dosage form of the present invention is unique, and application mode is simply effective, is particularly suitable for the use of newborn young stock.
By clinical trial of the present invention, find out, the present invention's curative effect aspect the newborn pneumoenteritis of calves of control is certain, the present invention prevents and treats diarrhoea with probiotic bacteria, the problems such as drug resistance, drug residue and weak curative effect of effectively having avoided application antibiotic and chemicals to produce.The present invention simultaneously combines the feature of newborn young stock medication, and medicine is made to spray, when application, is directly sprayed onto on the body surface and breast of female animal, and young stock just can contact probiotic bacteria when eating colostrum, reaches the object of rapid onset.Application process of the present invention is simple, convenient, effective, is adapted at applying in animal husbandry production.
The specific embodiment
Below in conjunction with example, the present invention will be further described, but the present invention is not limited.
Embodiment 1
The preparation method of the compound probiotic spray of the present embodiment is as follows:
1) Bacillus subtillis, Bacillus cereus, enterococcus faecalis, yeast are inoculated into respectively separately in optimum medium, cultivate 24 hours for 37 ℃, respectively get culture fluid 100ml and be transferred in fermentation tank and carry out amplification culture, in fermentation liquid, viable bacteria concentration reaches 2 * 10
8when CFU/ml is above, stop cultivating.Get fermentation liquid and carry out centrifugally, 3000 revs/min are carried out centrifugal 20 minutes, collect thalline.
2) by above-mentioned thalline, be 1: 1: 2.5 by weight: 2 evenly mix, get 100 parts, mixture, 300 parts of defatted milk powder, 50 parts of mannose, 50 parts of evenly mixing of cysteine, and stir 30 minutes by the speed of 50 revs/min, thalline is thoroughly adsorbed onto on carrier.
3) get 0 part of DEXTRIN, 400 parts of sodium alginates and step 2) gained mixture mixes, and by step 2) method stir 30 minutes, then to spraying into 100 parts of 5% sucrose solutions in mixture, continue to stir 30 minutes, room temperature aeration-drying, packs and get final product.
Embodiment 2
The preparation method of the compound probiotic spray of the present embodiment is as follows:
1) Bacillus licheniformis, lactobacillus, bacillus bifidus, streptococcus thermophilus are inoculated into respectively separately in optimum medium, cultivate 36 hours for 37 ℃, respectively get culture fluid 100ml and be transferred in fermentation tank and carry out amplification culture, in fermentation liquid, viable bacteria concentration reaches 2 * 10
8when CFU/ml is above, stop cultivating.Get fermentation liquid and carry out centrifugally, 4000 revs/min are carried out centrifugal 15 minutes, collect thalline.
2) by above-mentioned thalline, be 0.5: 1.5: 2 by weight: 1 evenly mixes, get 100 parts, mixture, 200 parts of defatted milk powder, 200 parts of soybean protein powders, 50 parts of evenly mixing of sodium lactate, and stir 30 minutes by the speed of 50 revs/min, thalline is thoroughly adsorbed onto on carrier.
3) get 600 parts of soluble starches, 200 parts of mannose, 200 parts of zeolite powders and step 2) mixing of gained mixture, and by step 2) method stir 30 minutes, then to spraying into 300 parts of 8% sucrose solutions in mixture, continue to stir 30 minutes, room temperature aeration-drying, packs and get final product.
Embodiment 3
The preparation method of the compound probiotic spray of the present embodiment is as follows:
1) Bacillus cereus, bacillus bifidus, enterococcus faecalis, Lactobacillus plantarum, streptococcus thermophilus are inoculated into respectively separately in optimum medium, cultivate 24 hours for 37 ℃, respectively get culture fluid 100ml and be transferred in fermentation tank and carry out amplification culture, in fermentation liquid, viable bacteria concentration reaches 2 * 10
8when CFU/ml is above, stop cultivating.Get fermentation liquid and carry out centrifugally, 4500 revs/min are carried out centrifugal 30 minutes, collect thalline.
2) by above-mentioned thalline, be 1: 2: 2.5 by weight: evenly mix at 1: 1, get 200 parts, mixture, 100 parts of defatted milk powder, 300 parts of soybean protein powders, 100 parts of evenly mixing of cysteine, and stir 30 minutes by the speed of 50 revs/min, thalline is thoroughly adsorbed onto on carrier.
3) get 600 parts of glucoses, 600 parts of soluble starches, 300 parts of sodium alginates and step 2) mixing of gained mixture, and by step 2) method stir 30 minutes, then to spraying into 400 parts of 2% sucrose solutions in mixture, continue to stir 30 minutes, room temperature aeration-drying, packs and get final product.
Test example 1:
This test example is the effect test of compound probiotic spray to newborn piglet that prevents and treat newborn diarrhea in livestock according to prepared a kind of of embodiment.
1.1 test material
Tested medicine: the spray of the embodiment of the present invention, the probiotic composition in embodiment (Bacillus subtillis, Bacillus cereus, enterococcus faecalis, yeast), coated carrier (dextrin, sodium alginate), absorption carrier (defatted milk powder, mannose, cysteine), coated carrier and absorption carrier mixture, carrier mixed proportion is all prepared according to embodiment.
Experimental animal: the long x Dasanyuan hybridization of 1 age in days Du x piglet.
1.2 test method
1.2.1 animal grouping is divided into 5 groups at random by 75 the long x Dasanyuan hybridization of 1 age in days Du x piglets, be experimental group I (preparation of the present invention), experimental group II (coated carrier), experimental group III (absorption carrier), experimental group IV (coated carrier and absorption carrier mixture) and matched group, 15 every group.
1.2.2 test method
Medicine in test is evenly mixed with water in the ratio of 1: 100, pack in aerosol apparatus, be directly sprayed on breast and the body surface of sow, every day 4 times, successive administration is until wean (30 age in days).In the diarrhoea situation of the overall process observed and recorded piglet of testing, calculate diarrhea rate and diarrhea frequency; Before test and after finishing, respectively to each group piglet measured body weight, determine gain in weight.Diarrhea rate=diarrhoea number/total number * 100%, diarrhea frequency=(diarrhoea number * diarrhoea natural law)/(test number * test natural law) * 100%.
1.3 result of the tests:
Various embodiments of the present invention test effect is in Table 1, as can be seen from Table 1, after using product of the present invention, can significantly reduce incidence rate and the diarrhea frequency of newborn piglet diarrhoeal diseases, and aspect the average weight gain of piglet, test group I is significantly higher than other test group and matched group.
Conclusion: composite probiotics preparations of the present invention makes probiotics field planting rapidly in newborn piglet intestinal, adjust intestinal microecology balance, improve immunity and the resistance against diseases of piglet, can effectively prevent and treat newborn piglet diarrhoea, can significantly improve the growth performance of newborn piglet simultaneously.Dosage form of the present invention is spray, has overcome the shortcomings such as the administration of existing dosage form is inconvenient, and dosage is large, drug cost height, is suitable for applying in aquaculture.
Claims (5)
1. a compound probiotic spray of preventing and treating newborn diarrhea in livestock, is characterized in that: the raw material by following weight portion is prepared from: absorption carrier 200-1000 part, coated carrier 400-2000 part, probiotic combinations 100-200 part.
2. according to the compound probiotic spray of preventing and treating newborn diarrhea in livestock described in claim 1, it is characterized in that: described probiotic bacteria is the combination of at least three kinds in Bacillus subtillis, Bacillus licheniformis, Bacillus cereus, lactobacillus, bacillus bifidus, enterococcus faecalis, Lactobacillus plantarum, yeast and streptococcus thermophilus.
3. according to the compound probiotic spray of preventing and treating newborn diarrhea in livestock described in claim 1, it is characterized in that: described absorption carrier is defatted milk powder, soybean protein powder, trehalose, sodium lactate, any in cysteine, two or more.
4. according to the compound probiotic spray of preventing and treating newborn diarrhea in livestock described in claim 1, it is characterized in that: described coated carrier be in sucrose, glucose, soluble starch, dextrin, essence, mannose, zeolite powder, sodium alginate any, two or more.
5. the preparation method of preventing and treating the compound probiotic spray of newborn diarrhea in livestock described in claim 1, is characterized in that:
1) probiotic bacteria is inoculated into separately in optimum medium, cultivates under suitable condition after 18-48 hour and be transferred in fermentation tank and cultivate in a large number, in fermentation liquid, viable bacteria concentration reaches 2 * 10
8during CFU/ml, get fermentation liquid and carry out high speed centrifugation, rotating speed is 3000～6000 revs/min, after collection thalline, mixes by a certain percentage.
2) by absorption carrier 110 ℃ of autoclavings 15 minutes, by certain weight ratio and step 1) gained probiotic bacteria mixes, the speed by 50 revs/min in the environment lower than 20 ℃ stirs 30～60 minutes, and thalline is thoroughly adsorbed onto on carrier.
3) get coated carrier and step 2) gained mixture mixes by a certain percentage, speed by 50 revs/min in the environment lower than 20 ℃ stirs 30 minutes, (volume ratio 1: 10～20) sprays into 2%～8% sucrose solution in mixture more in proportion, continue to stir 30 minutes, after room temperature aeration-drying, pack and get final product.
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| CN106635917A (en) * | 2017-01-13 | 2017-05-10 | 浙江恒然环保科技有限公司 | Functional compound fungicide used for kitchen waste and having low-temperature efficient degradation capability |
| CN107007633A (en) * | 2016-01-27 | 2017-08-04 | 聊城市畜牧兽医局 | A kind of probiotics polysaccharide composite formulation preparation method |
| CN107279469A (en) * | 2017-07-03 | 2017-10-24 | 青岛玛斯特生物技术有限公司 | A kind of complex microorganism preparations and its after aquatic feeds spray in apply |
| CN109007409A (en) * | 2018-07-06 | 2018-12-18 | 华南农业大学 | A kind of hatching Broiler chicks Synbiotics mouthspray preparation and preparation method thereof |
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| CN103349186A (en) * | 2013-08-01 | 2013-10-16 | 正大康地（澄海）有限公司 | Weaned piglet feed additive and preparation method thereof |
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2014
- 2014-04-24 CN CN201410176927.0A patent/CN103919816B/en active Active
Patent Citations (3)
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| CN101112397A (en) * | 2007-08-09 | 2008-01-30 | 天津科技大学 | Viable prebiotics bacteria microcapsule preparations for livestock and method for preparing the same |
| CN103283975A (en) * | 2013-03-27 | 2013-09-11 | 广州格拉姆生物科技有限公司 | A method of using spray drying for preparing probiotics micro-capsules |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107007633A (en) * | 2016-01-27 | 2017-08-04 | 聊城市畜牧兽医局 | A kind of probiotics polysaccharide composite formulation preparation method |
| CN106635917A (en) * | 2017-01-13 | 2017-05-10 | 浙江恒然环保科技有限公司 | Functional compound fungicide used for kitchen waste and having low-temperature efficient degradation capability |
| CN107279469A (en) * | 2017-07-03 | 2017-10-24 | 青岛玛斯特生物技术有限公司 | A kind of complex microorganism preparations and its after aquatic feeds spray in apply |
| CN107279469B (en) * | 2017-07-03 | 2021-05-28 | 青岛玛斯特生物技术有限公司 | Compound microbial preparation and application thereof in post-spraying of aquatic feed |
| CN109007409A (en) * | 2018-07-06 | 2018-12-18 | 华南农业大学 | A kind of hatching Broiler chicks Synbiotics mouthspray preparation and preparation method thereof |
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