AU2016297405B2 - Bacillus pumilus strain and microbial ecological combined agent thereof for weaned piglet - Google Patents
Bacillus pumilus strain and microbial ecological combined agent thereof for weaned piglet Download PDFInfo
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- AU2016297405B2 AU2016297405B2 AU2016297405A AU2016297405A AU2016297405B2 AU 2016297405 B2 AU2016297405 B2 AU 2016297405B2 AU 2016297405 A AU2016297405 A AU 2016297405A AU 2016297405 A AU2016297405 A AU 2016297405A AU 2016297405 B2 AU2016297405 B2 AU 2016297405B2
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- 230000000813 microbial effect Effects 0.000 title claims description 30
- 241000194103 Bacillus pumilus Species 0.000 title claims description 18
- 230000001580 bacterial effect Effects 0.000 claims description 16
- 241000193749 Bacillus coagulans Species 0.000 claims description 13
- 229940054340 bacillus coagulans Drugs 0.000 claims description 13
- 241000894007 species Species 0.000 claims description 13
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 12
- 241000194108 Bacillus licheniformis Species 0.000 claims description 8
- 244000063299 Bacillus subtilis Species 0.000 claims description 8
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 3
- 206010012735 Diarrhoea Diseases 0.000 description 16
- 238000012360 testing method Methods 0.000 description 15
- 238000002360 preparation method Methods 0.000 description 13
- 239000000047 product Substances 0.000 description 11
- 239000003795 chemical substances by application Substances 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 239000006041 probiotic Substances 0.000 description 3
- 235000018291 probiotics Nutrition 0.000 description 3
- 108020004465 16S ribosomal RNA Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000036528 appetite Effects 0.000 description 2
- 235000019789 appetite Nutrition 0.000 description 2
- 235000021052 average daily weight gain Nutrition 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 235000019621 digestibility Nutrition 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000006996 mental state Effects 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 235000019786 weight gain Nutrition 0.000 description 2
- 230000004584 weight gain Effects 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 241000193171 Clostridium butyricum Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- 230000009102 absorption Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 229940089206 anhydrous dextrose Drugs 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000007596 consolidation process Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 231100000517 death Toxicity 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000008376 long-term health Effects 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
Provided is a
Description
BACILLUS PUMILUS STRAIN AND MICROBIAL ECOLOGICAL
COMBINED AGENT THEREOF FOR WEANED PIGLET
TECHNICAL FIELD [0001] The present invention relates to the field of microbial technology, in particular to a
Bacillus pumilus strain, and an application thereof beneficial for a weaned piglet.
BACKGROUND [0002] A nursery piglet is referred to as a weaned piglet, the pig at this stage is very fragile, and various diseases, even massive deaths easily occur, since the maternal antibody disappears, the new immune system has not yet been established, besides, there are other reasons, such as a rapid growing speed and many stress factors. Although antibiotics and chemicals can solve the emerging problem in a relatively short period of time, drug-resistance and drug residue problems caused therefrom may have an impact on the late growth of the piglet. How to improve the health condition, survival rate, and uniformity of the weaned piglet as well as how to fully play aftereffects of growth are problems commonly concerned by every farmer.
[0003] The microbial ecological preparation has roles of regulating and controlling animal intestinal microbial balance, proliferating beneficial bacteria, inhibiting harmful bacteria, reducing intestinal PH values, improving the enzyme activity in the digestive tract, nutrient digestibility and the organism immunity, and etc. Reports on the application of the microbial ecological preparation in piglet breeding are increasing. CN 101703136B reports an application of the Lactobacillus plantarum viable bacteria preparation in preventing and treating piglet diarrhea, the efficiency rate for the piglet diarrhea is more than 90% and the cure rate is more than 80%. CN 103255080B reports an application of the Bacillus coagulans (Bacillus coagulans HM-09) in the preparation of feedstuff for improving the productive performance and diarrhea of piglets, the occurrence rate of diarrhea of a weaned piglet can be reduced, thus the occurrence of enterogenous diseases can be reduced, the feedstuff digestibility is improved, and the utilization rate of the piglet feedstuff is improved. CN 103205373B reports a strain of Clostridium butyricum for breeding a piglet, which can antagonize animal pathogenic bacteria in the intestinal tract of the piglet, so as to maintain and regulate the microbial ecological balance, enhance the immune function of the organism, produce amylase and vitamins, and promote digestion and absorption and nutrition effects of the animals on the feedstuff.
[0004] In general, the microbial ecological preparation is slow in effect and capable of receiving a good effect after being used consistently in the livestock breeding process. Therefore, there is a need to develop a microbial ecological preparation capable of a long-term health care, but also quickly solving problems of piglet diarrhea, loose bowels, etc., and it is particularly necessary for realizing stable production of the weaned piglets.
SUMMARY [0005] In view of the above problems in the prior art, the applicant provides a Bacillus pumilus strain and a microbial ecological combined agent for a weaned piglet. Through the present invention, not only problems of piglet diarrhea, loose bowels, etc can be solved quickly, but also the health level of a weaned piglet can be improved during a long-term use. [0006] Technical solutions of the present invention are as follows:
[0007] A Bacillus pumilus strain ZS008 is deposited in the China Center for Type Culture Collection (CCTCC) on July 13, 2015 with a deposit number of CCTCC No. M2015451.
[0008] A microbial ecological Bacillus combined agent for a weaned piglet is combined and formulated by the following bacterial species: a Bacillus pumilus (CCTCC No. M2015451), a Bacillus coagulans (CCTCC No. M2015273), a Bacillus subtilis (CCTCC No. M2011443) and a. Bacillus licheniformis (CCTCC No. M2012458).
[0009] The microbial ecological Bacillus combined agent has an effective viable count of
10
1.4x 10 -7.0x 10 cfu/g, and the bacterial species composition is as follows:
Bacterial species
Bacillus pumilus CCTCC No. M2015451
Viable count of bacterial species per gram of product, in cfu/g
O.4xlO8-2.OxlO10
Bacillus coagulans CCTCC No. M2015273
Bacillus subtilis CCTCC No. M 2011443
O.4x1O8-2.Ox1O10
O.4x1O8-2.Ox1O10
O.2x1O8-1.Ox1O10
Bacillus licheniformis CCTCC No. M 2012458 [0010] VcizBacillus coagulans is Bacillus coagulans ZS007 is deposited in the China
Center for Type Culture Collection with a deposit number of CCTCC No. M2015273.
[0011] An application of the microbial ecological Bacillus combined agent for a weaned piglet is used for improving the productive performance of the weaned piglet. The dosage thereof is 1.0-5.Og/head/day.
[0012] Single bacterium powders of Bacillus subtilis CCTCC No. M 2011443 and
Bacillus licheniformis CCTCC No. M 2012458 are commercially available from Taizhou
Lvshengyuan Biotechnology Co., Ltd. The product specifications thereof are both 5.OxlOlocfu/g.
[0013] The beneficial technical effects of the prevent invention are as follows:
[0014] 1) Problems of piglet diarrhea, loose bowels, etc can be solved quickly;
[0015] 2) After a long-term use, the health level of a weaned piglet can be improved, and thus the effect of improving production capacity of the weaned piglet can be achieved.
DETAILED DESCRIPTION [0016] 1. Screening of Bacillus pumilus [0017] 2g of soil sample near Jiangsu Wuxi Fangdingding Pig Farm is weighed, and added with 98 ml of sterile physiological saline, after heat treatment for 10 minutes at the temperature of 80°C, oscillated in a rotary shaker(120 rpm) at 37°C for 20 min, 1 ml of the supernatant is aspirated and added to LB culture medium for dilution and coating, so as to isolate bacterial strains. Genuses and species of bacterial species are determined effectively and accurately taking means, such as morphological observation, physiological biochemical experiment, 16S rDNA analysis on bacterial strains.
[0018] Finally, a strain with a serial number of ZS008 is obtained, and identified as a Bacillus pumilus. The strain is deposited in the China Center for Type Culture Collection on July 13, 2015 with a deposit number of CCTCC No. M2015451.
[0019] 2. Screening of Bacillus coagulans [0020] 2g of soil sample near a certain dairy farm in Mashan, Wuxi, Jiangsu is weighed, and added with 98 ml of sterile physiological saline, after heat treatment for 10 minutes at the temperature of 80°C, oscillated in a rotary shaker(120 rpm) at 37°C for 20 min, 1 ml of the supernatant is aspirated and added to LB culture medium for dilution and coating, so as to isolate bacterial strains. Genuses and species of bacterial species are determined effectively and accurately taking means, such as morphological observation, physiological biochemical experiment, 16S rDNA analysis on bacterial strains.
[0021] Finally, a strain with a serial number of ZS007 is obtained, and identified as a Bacillus coagulans.
[0022] ZS007is deposited in the China Center for Type Culture Collection on May 3, 2015 with a deposit number of CCTCC M 2015273.
[0023] 3. Preparation of a microbial ecological combined agent [0024] The microbial ecological combined agent is combined and formulated by a Bacillus pumilus (CCTCC No. M2015451), a Bacillus coagulans (CCTCC No. M2015273), a Bacillus subtilis (CCTCC No. M2011443) and a Bacillus licheniformis (CCTCC No. M2012458).
[0025] In particular, single bacterium powders of Bacillus subtilis CCTCC No. M 2011443 and Bacillus licheniformis CCTCC No. M 2012458 are commercially available from Taizhou Lvshengyuan Biotechnology Co., Ltd. The product specifications thereof are both 5.OxlOlocfir/g.
[0026] With respect to microbial ecological preparation products prepared in Embodiment 1 to Embodiment 3, corresponding microbial contents are as shown in Table 1 below:
[0027] Table 1
Bacterial species
Viable count of bacterial species per gram of product, in cfu/g
Bacillus pumilus CCTCC No.
M2015451
Bacillus coagulans CCTCC No.M
2015273
Bacillus subtilis CCTCC No. M
2011443
Embodiment 1
l.OxlO8
l.OxlO8
l.OxlO8
Bacillus licheniformis CCTCC No. M 0 ' l.OxlO8
2012458
Viable count 4.0xl08
Embodiment 2
l.OxlO9
l.OxlO9
l.OxlO9
l.OxlO9
4.0xl09
Embodiment 3
l.OxlO10
2.0xl0w
2.0xl0w
2.0xl0w
7-OxlO10 [0028] Single bacterium powders are uniformly mixed in a given proportion, and added with anhydrous dextrose as a carrier, to supplement to 100% of the total weight, by taking Bacillus as an effective component, so that the total weight of the product can meet that the
O final viable count reaches 4.0*10 cfu/g, after the carrier is added, and the mixture is uniformly mixed for later use.
[0029] Make sure the operating environment is clean during processing, so as to avoid contamination. The environment humidity is controlled below 70%, the moisture content of each raw material is lower than 13%, and the prepared product can be stored at normal temperature for 24 months, and the storage life can reach more than 60 months at -18°C, and the viable count is stable within the shelflife.
[0030] 4. Application effect of probiotic bacteria combined agent on a weaned piglet [0031] 90 Duroc X Landrace X Yorkshire three-way crossbred weaned piglets are selected from the same batch of self-reproductive self-breading piglets, and divided into three groups A, B, C in the same or similar proportions of parity, weight and gender, Group A is a control group, Group B is a test group 1, Group C is a test group 2, each group has 2 replicates with 15 piglets per replicate, piglets are divided randomly and numbered one by one; each group is fed with the same basic rations (Zhengda complete feedstuff), with consistent nutritional levels. No enzyme preparation is added in Group A, a microbial ecological combined agent containing a Bacillus pumilus (Embodiment 1) is added on the basis of basic rations in Group
B, and Group C is the same with Group B, except that there is no Bacillus pumilus', the dosage of the microbial ecological preparation is 1.0-5.Og/head/day. The piglets are weaned at 21 days old under an average weight of 6.5kg, the pre-test is 7 days and feeding test is 30 days. The breeding site is a certain breeding cooperative in Nanjing.
[0032] Test results are as shown in Table 2 to Table 4 below.
[0033] Table 2 Weight gain in the test group and average daily weight gain within the test period
| Group | Average initial weight/(kg/head) | Average final weight/(kg/head) | Average daily weight gain/(g/head) |
| Control group | 7.46±0.33 | 21.43±0.82 | 467±22 |
| Test group 1 | 7.41±0.28 | 23.79±0.94 | 544±28 |
| Test group 2 | 7.43±0.31 | 22.64±0.84 | 502±27 |
[0034] Table 3 Feed conversion efficiency in each test group after feeding enzyme-added
| probiotics | ||||
| Group | Total feedstuff consumption/kg | Daily feedstuff consumption/kg | Total weight gain/kg | Feed/gain ratio |
| Control group | 653.4 | 21.78 | 420.57 | 1.55 |
| Test group 1 | 676.7 | 22.56 | 489.78 | 1.38 |
| Test group 2 | 665.3 | 22.18 | 456.36 | 1.46 |
| [0035] Table | 4 Frequency of | piglet diarrhea | in each test group | after feeding |
enzyme-added probiotics
| Group | Daily diarrhea headage | Daily feeding headage | Diarrhea rate (%) |
| Control group | 53 | 900 | 5.89 |
| Test group 1 | 23 | 900 | 2.56 |
| Test group 2 | 34 | 900 | 3.78 |
[0036] After piglet diarrhea occurs, 5g of microbial ecological preparation product is used to each piglet for treatment. After being stirred with 10 mL of cold boiled water, the product is drawn into a syringe, and drenched in the corner of the piglet’s mouth with the syringe. When 15 the microbial ecological preparation product contains Bacillus pumilus, the mental state of the piglet gets better after 4 to 6 hours, the piglet diarrhea disappears within 24 hours, and the appetite returns to normal. When the microbial ecological preparation product doesn’t contain Bacillus pumilus, the mental state of the piglet gets better after 12 hours; consolidation treatment is carried out once, the piglet diarrhea disappears after about 2 days, and the 5 appetite returns to normal.
Claims (6)
- THE CLAIMS DEFINING THE INVENTION ARE AS FOLLOWS:1. A Bacillus pumilus strain ZS008, deposited in the China Center for Type Culture Collection on July 13, 2015 with a deposit number of CCTCC No. M2015451, in an isolated form.
- 2. A microbial ecological Bacillus combined agent for a weaned piglet, characterized in that, being combined and formulated by the following bacterial species: a Bacillus pumilus strain ZS008, deposited in the China Center for Type Culture Collection on July 13, 2015 with a deposit number of CCTCC No. M2015451, a Bacillus coagulans (CCTCC No. M2015273), a Bacillus subtilis (CCTCC No. M2011443) and a Bacillus licheniformis (CCTCC No. M2012458).
- 3. The microbial ecological Bacillus combined agent for a weaned piglet according to claim 2, characterized in that the microbial ecological Bacillus combined agent has an effective viable count of 1.4x10 -7.0χ10 cfu/g, and the bacterial species composition is as follows:Bacterial speciesBacillus pumilus CCTCC No. M2015451Bacillus coagulans CCTCC No. M2015273Bacillus subtilis CCTCC No. M 2011443Bacillus licheniformis CCTCC No. M 2012458Viable count of bacterial species per gram of product, in cfu/g O.4xlO8-2.OxlO10O.4x1O8-2.Ox1O10O.4x1O8-2.Ox1O10O.2x1O8-1.Ox1O10
- 4. The microbial ecological Bacillus combined agent for a weaned piglet according to claim 2, characterized in that the Bacillus coagulans is Bacillus coagulans ZS007, deposited in the China Center for Type Culture Collection with a deposit number of CCTCC No.M2015273.
- 5. An application of the microbial ecological Bacillus combined agent for a weaned piglet according to claim 2, characterized in that the application of the microbial ecological Bacillus combined agent for a weaned piglet is used for improving the productive2016297405 13 Dec 2018 performance of the weaned piglet.
- 6. The application of the microbial ecological Bacillus combined agent for a weaned piglet according to claim 5, characterized in that the microbial ecological Bacillus combined agent has a dosage of 1.0-5.Og/head/day.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510436127.2 | 2015-07-22 | ||
| CN201510436127.2A CN104974967B (en) | 2015-07-22 | 2015-07-22 | One bacillus pumilus and its child care piglet compound micro-ecological preparation |
| PCT/CN2016/090899 WO2017012573A1 (en) | 2015-07-22 | 2016-07-21 | Bacillus pumilus strain and microbial ecological combined agent thereof for weaned piglet |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2016297405A1 AU2016297405A1 (en) | 2017-09-21 |
| AU2016297405B2 true AU2016297405B2 (en) | 2019-01-03 |
Family
ID=54271934
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2016297405A Ceased AU2016297405B2 (en) | 2015-07-22 | 2016-07-21 | Bacillus pumilus strain and microbial ecological combined agent thereof for weaned piglet |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN104974967B (en) |
| AU (1) | AU2016297405B2 (en) |
| WO (1) | WO2017012573A1 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104974967B (en) * | 2015-07-22 | 2018-04-17 | 江南大学 | One bacillus pumilus and its child care piglet compound micro-ecological preparation |
| TWI569731B (en) * | 2015-12-30 | 2017-02-11 | 聯發生物科技股份有限公司 | Bacterial additive for animal food and producing method thereof |
| CN106922951A (en) * | 2015-12-30 | 2017-07-07 | 联发生物科技股份有限公司 | Feed addition microbial inoculum and preparation method thereof, bacterial strain screening method |
| EP3745876A1 (en) * | 2018-02-02 | 2020-12-09 | Novozymes A/S | Management of pathogenic lawsonia |
| CN111548965B (en) * | 2020-05-20 | 2022-03-18 | 山东省农业科学院 | Donkey-derived bacillus pumilus and application thereof in preparation of medicine for treating diarrhea of donkey colt |
| CN114272552A (en) * | 2021-12-06 | 2022-04-05 | 紫茎泽兰科技(云南)有限公司 | Method for detoxifying Eupatorium adenophorum |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013029013A1 (en) * | 2011-08-24 | 2013-02-28 | Dupont Nutrition Biosciences Aps | Enzyme producing bacillus strains |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101669576B (en) * | 2008-09-12 | 2013-02-20 | 安琪酵母股份有限公司 | Yeast composition and feed |
| CN102505003B (en) * | 2011-12-19 | 2013-06-26 | 湖南省微生物研究所 | High-density fermenting method of bacillus coagulans for livestock and poultry, as well as preparation prepared by method and application thereof |
| CN103255080B (en) * | 2013-03-22 | 2015-05-27 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for breeding piglets and application thereof |
| CN105211638A (en) * | 2014-05-26 | 2016-01-06 | 瑞普（天津）生物药业有限公司 | A kind of probiotics of prevention and therapy breast piglet bacillary diarrhoea |
| CN104171756B (en) * | 2014-09-02 | 2015-11-18 | 双胞胎（集团）股份有限公司 | Family's pig farm nursery-stage pig feed and feeding pattern thereof |
| CN104263683B (en) * | 2014-09-15 | 2017-09-29 | 中国农业科学院饲料研究所 | Bacillus pumilus 315 and its application with prebiotic effect |
| CN104472862A (en) * | 2014-12-29 | 2015-04-01 | 江苏千药堂国医研究院有限公司 | Biological feed additive and preparation method thereof |
| CN104974967B (en) * | 2015-07-22 | 2018-04-17 | 江南大学 | One bacillus pumilus and its child care piglet compound micro-ecological preparation |
| CN105341451A (en) * | 2015-10-26 | 2016-02-24 | 滁州市南谯区施集镇兴牧生态养殖场 | Special feed capable of promoting digestion and preventing diarrhoea for weaned piggies |
-
2015
- 2015-07-22 CN CN201510436127.2A patent/CN104974967B/en active Active
-
2016
- 2016-07-21 WO PCT/CN2016/090899 patent/WO2017012573A1/en active Application Filing
- 2016-07-21 AU AU2016297405A patent/AU2016297405B2/en not_active Ceased
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013029013A1 (en) * | 2011-08-24 | 2013-02-28 | Dupont Nutrition Biosciences Aps | Enzyme producing bacillus strains |
Non-Patent Citations (1)
| Title |
|---|
| DAI, RONGGUO et al., "To Compare the Effect of Four Kind Bacilli Composites on Performance of Piglets", GANSU ANIMAL AND VETERINARY SCIENCES, (2006-01-31), vol. 36, no. 1, ISSN 1006-799X, pages 14 - 16 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017012573A1 (en) | 2017-01-26 |
| CN104974967A (en) | 2015-10-14 |
| AU2016297405A1 (en) | 2017-09-21 |
| CN104974967B (en) | 2018-04-17 |
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