CN103621792A - Feed additive containing L-lysine composite probiotics and preparation method thereof - Google Patents
Feed additive containing L-lysine composite probiotics and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a feed additive containing L-lysine composite probiotics, which is composed of the following components in percentage by weight: 1-3% of L-lysine powder and 97-99% of composite probiotic powder. The additive can enhance the utilization ratio of proteins, promote the growth of livestock and fowl, and improve the constitution of livestock and fowl. The feed product with the additive is a combination of multiple strains belonging to multiple bacterial genera, has the advantages of high strain stability, long storage time, favorable permanent planting capacity in intestinal tracts of livestock and fowl and abundant nutrition, promotes the appetite of livestock and fowl, enhances the disease resistance of livestock fowl, promotes the growth development of livestock and fowl, and increases the utilization ratio of the feed.
Description
Technical field
The present invention relates to a kind of probiotic feed additive, especially relate to feed addictive of a kind of 1B compound probiotic and preparation method thereof.
Background technology
From the fifties in last century, in animal feed, add antibiotic and significantly promoted growth of animal, and the development of intensive animal husbandry has been made to major contribution.Yet As time goes on, in feed, add antibiotic harm and day by day manifest, and be subject to social extensive concern.European Union completely forbids and in animal and fowl fodder, adds antibiotic from January, 2006.People start to seek one after another other substitute and substitute technology, unaffected to guarantee efficiency and the benefit of animal husbandry production.
Fermented feed containing probiotics technology is the novel fodder technology with many advantages of recently growing up.Probiotic feed additive is to utilize the processes such as beneficial functions microorganism mixes with premixed feed process, fermentation, low temperature drying to form the high-quality feed additive containing the safety non-pollution drug residue free of active probiotic.Because it has BA, can be good at participating in the ecological balance of animal intestines and stomach microorganism species, promote digesting and assimilating of animal, improve food conversion ratio and production performance; Strengthen the resistance against diseases of livestock and poultry simultaneously, be therefore acknowledged as the non-harmful green feed additive of a kind of novel high-quality.
Lysine is one of important component of protein, is that animal body can not self be synthetic but one of eight seed amino acids of extremely needing are a kind of good food additives.Because lacking lysine in food, so claim again " the first essential amino acid ".Lysine is added to the utilization rate that can improve protein in feed, thereby the nutrition of condensed food is greatly played enhancing development, increases appetite, is reduced disease, constitutional effect.
At present, China's additive for microbe feedstuff is of a great variety, but most products bacterial classification is more single, and only, containing a kind of bacterium, function is also more single, bacterial classification less stable, and storage life is short, and can not well field planting in animal and bird intestines, and effect is not ideal.Lysine and compound probiotic are added in feed to more rarely seen as additive simultaneously.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of feed addictive of 1B compound probiotic, and this additive, for animal feed, can be improved to the utilization rate of protein, promotes growth of animals or poultry, strengthens livestock and poultry physique.
The technical solution used in the present invention is to provide a kind of feed addictive of 1B compound probiotic, and this additive is comprised of the following component of percentage by weight, 1B powder 1-3%, compound probiotic powder 97-99%.
The present invention also provides the method for preparing this additive, it is characterized in that comprising following method:
(1) preparation of 1B powder: get corynebacterium glutamicum bacterial strain, be inoculated in fluid nutrient medium, activation, shaking table is cultivated 24h, obtains corynebacterium glutamicum nutrient solution; After water dissolves peptone, add after bean cake powder, megasse mix and blend, sterilizing 1 hour, be cooled to 30 ℃, access corynebacterium glutamicum nutrient solution expands cultivation again, and fermentation 72h, obtains 1B liquid, by ion-exchange, extract 1B, through crystallization, the dry 1B powder that obtains;
(2) preparation of compound probiotic nutrient solution: after water dissolves glucose, sucrose, potassium dihydrogen phosphate, add after bean cake powder, megasse mix and blend, in 120 ℃ of sterilizings 30 minutes, be cooled to 30 ℃, access respectively bacillus subtilis, lactobacillus acidophilus, candida utili bacterium and excrement intestines ball slant strains and carry out pure culture, after cultivation finishes, by equal-volume, mix, be mixed with compound probiotic nutrient solution;
(3) preparation of composite probiotics ferment liquid: water adds after bean cake powder, megasse mix and blend after brown sugar, potassium dihydrogen phosphate are dissolved, and sterilizing 1 hour, is cooled to 30 ℃, and access compound probiotic nutrient solution, obtains composite probiotics ferment liquid;
(4) preparation of compound probiotic powder: by corn flour, bean cake powder mixing and stirring, after water dissolves brown sugar, in 100 ℃ of sterilizings 1 hour, treat that temperature is down to 30 ℃, then after adding composite probiotics ferment liquid to mix, be evenly sprayed on the mixture of corn flour and bean cake powder and stir, make the mixture of corn flour and bean cake powder keep moisture, aerobic fermentation 5 days, and then anaerobic fermentation 2 days are dried and are pulverized to obtain compound probiotic powder at 50 ℃ of temperature;
(5) 1B powder and compound probiotic powder are mixed in proportion to the composite probiotics feed additive of making containing 1B.
Preferably, the described activation temperature of step (1) is 30-34 ℃, and described sterilising temp is 100-120 ℃, and the temperature that described nutrient solution expands cultivation is again controlled at 30-32 ℃; Preferably, described activation temperature is 30-32 ℃, and described sterilising temp is 110-120 ℃, and the temperature that described nutrient solution expands cultivation is again controlled at 30 ℃.
Preferably, the described pure culture of step (2) is, cultivates 48 hours for bacillus subtilis 45-50 ℃, cultivates 72 hours for lactobacillus acidophilus 35-37 ℃, and 25-30 ℃ of candida utili bacterium cultivated 48 hours, cultivates 48 hours for enterococcus faecalis 35-40 ℃.
Preferably, the described sterilising temp of step (3) is 100 ℃-120 ℃, and the incubation time that described access compound probiotic nutrient solution obtains composite probiotics ferment liquid is 72-80 hour, and it is 30 ℃-35 ℃ that temperature is controlled.
Preferably, the mixture moisture of the described corn flour of step (4) and bean cake powder remains on 28-30%, and temperature is controlled at 30-35 ℃ of aerobic fermentation, and temperature is controlled 25-30 ℃ of anaerobic fermentation.
The invention has the beneficial effects as follows:
(1) product of the present invention contains 1B, can improve the utilization rate of protein, promotes growth of animals or poultry, strengthens livestock and poultry physique.
(2) a plurality of strain combinations of a plurality of Pseudomonas of product of the present invention, bacterial classification is stable, and long shelf-life has good colonization ability in animal and bird intestines, nutritious, promotes appetite of livestock and poultry, strengthens livestock and poultry resistance against diseases, promotes animal growth and development, improves efficiency of feed utilization.
The addition of product of the present invention in feed is in 1% ratio of mass fraction.
The specific embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment 1
(1) preparation of 1B powder: get 4 of corynebacterium glutamicum bacterial classifications, access in 2 kilograms of fluid nutrient mediums of the bacterium of having gone out, shaking table temperature arranges 30 ℃, and rotating speed arranges 1200r/h, cultivates 24h, standby; Get each 5 kilograms of 60 object bean cake powders, megasses, 0.5 kilogram of peptone, 2 kilograms of corynebacterium glutamicum nutrient solutions, 87.5 kilograms, water.After first peptone water being dissolved, add bean cake powder, megasse mix and blend even, in 120 ℃ of sterilizings 30 minutes, be cooled to 30 ℃, access corynebacterium glutamicum nutrient solution expands cultivation again, and temperature is controlled at 30 ℃, aerobic fermentation 72h, obtain 1B liquid, by ion-exchange, extract 1B, through crystallization, the dry 1B powder product that obtains.
(2) preparation of compound probiotic nutrient solution: get 0.06 kilogram of glucose, 0.12 kilogram of sucrose, 0.06 kilogram of bean cake powder, 0.006 kilogram of potassium dihydrogen phosphate, 0.09 kilogram of megasse, each 2 of bacillus subtilis, lactobacillus acidophilus, candida utili inclined-plane bacterium and enterococcus faecalis, 2.664 kilograms, water.First water dissolves glucose, sucrose, potassium dihydrogen phosphate, then add after bean cake powder, megasse mix and blend, part classifies in three categories, 120 ℃ of sterilizings 30 minutes, be cooled to after 30 ℃, access respectively bacillus subtilis, lactobacillus acidophilus, candida utili inclined-plane bacterium and enterococcus faecalis kind, carry out pure culture; 45 ℃ of cultivations of bacillus subtilis 48 hours, 35 ℃ of cultivations of lactobacillus acidophilus 72 hours, 25 ℃ of cultivations of candida utili 48 hours, 35 ℃ of cultivations of enterococcus faecalis 48 hours, after fermentation ends, get 1 kilogram of l kilogram of bacillus subtilis liquid, l kilogram of lactobacillus acidophilus liquid, 1 kilogram of candida utili bacterium liquid, enterococcus faecalis bacterium liquid and mix, be mixed with compound probiotic nutrient solution.
(3) preparation of composite probiotics ferment liquid: get 12 kilograms of megasses, 10 kilograms, brown sugar, 2 kilograms of bean cake powders, 0.2 kilogram of potassium dihydrogen phosphate, 2 kilograms of compound probiotic nutrient solutions, 73.8 kilograms, water.After first water dissolves brown sugar, potassium dihydrogen phosphate, then add after megasse, bean cake powder mix and blend, 120 ℃ of sterilizings 1 hour, are cooled to 30 ℃, inoculation compound probiotic nutrient solution, and temperature is controlled at 30 ℃, cultivates 72 hours, obtains composite probiotics ferment liquid.
(4) preparation of compound probiotic powder: get 380 kilograms of 60 object corn flour, 300 kilograms of 60 object bean cake powders, brown sugar double centner, 20 kilograms of composite probiotics ferment liquid, 200 kilograms, water.First, by corn flour, bean cake powder mixing and stirring, then water dissolves brown sugar, 100 ℃ of sterilizings 1 hour, treat that temperature is down to 30 ℃, after adding again composite probiotics ferment liquid to mix, be evenly sprayed on the mixture of corn flour and bean cake powder and stir, make the mixture moisture of corn flour and bean cake powder remain on 28%, temperature is controlled at 30 ℃, aerobic fermentation 5 days, and then temperature is controlled to 25 ℃, anaerobic fermentation 2 days, at 50 ℃ of temperature, dry, pulverize to obtain compound probiotic powder.
(5) produce 1000 kilograms of feed addictives containing 1B compound probiotic, get 10 kilograms, 1B powder, 990 kilograms, compound probiotic powder mixes.
Embodiment 2
(1) preparation of 1B powder: get 4 of corynebacterium glutamicum bacterial classifications, access in 2 kilograms of fluid nutrient mediums of the bacterium of having gone out, shaking table temperature arranges 32 ℃, and rotating speed arranges 1200r/h, cultivates 24h, standby; Get each 5 kilograms of 60 object bean cake powders, megasses, 0.5 kilogram of peptone, 2 kilograms of corynebacterium glutamicum nutrient solutions, 87.5 kilograms, water.After first peptone water being dissolved, add bean cake powder, megasse mix and blend even, in 110 ℃ of sterilizings 30 minutes, be cooled to 30 ℃, access corynebacterium glutamicum nutrient solution expands cultivation again, and temperature is controlled at 31 ℃, aerobic fermentation 72h, obtain 1B liquid, by ion-exchange, extract 1B, through crystallization, the dry 1B powder product that obtains.
(2) preparation of compound probiotic nutrient solution: get 0.06 kilogram of glucose, 0.12 kilogram of sucrose, 0.06 kilogram of bean cake powder, 0.006 kilogram of potassium dihydrogen phosphate, 0.09 kilogram of megasse, each 2 of bacillus subtilis, lactobacillus acidophilus, candida utili inclined-plane bacterium and enterococcus faecalis, 2.664 kilograms, water.First water dissolves glucose, sucrose, potassium dihydrogen phosphate, then add after bean cake powder, megasse mix and blend, part classifies in three categories, 120 ℃ of sterilizings 30 minutes, be cooled to after 30 ℃, access respectively bacillus subtilis, lactobacillus acidophilus, candida utili inclined-plane bacterium and enterococcus faecalis kind, carry out pure culture; 48 ℃ of cultivations of bacillus subtilis 48 hours, 36 ℃ of cultivations of lactobacillus acidophilus 72 hours, 28 ℃ of cultivations of candida utili 48 hours, 38 ℃ of cultivations of enterococcus faecalis 48 hours, after fermentation ends, get 1 kilogram of l kilogram of bacillus subtilis liquid, l kilogram of lactobacillus acidophilus liquid, 1 kilogram of candida utili bacterium liquid, enterococcus faecalis bacterium liquid and mix, be mixed with compound probiotic nutrient solution.
(3) preparation of composite probiotics ferment liquid: get 12 kilograms of megasses, 10 kilograms, brown sugar, 2 kilograms of bean cake powders, 0.2 kilogram of potassium dihydrogen phosphate, 2 kilograms of compound probiotic nutrient solutions, 73.8 kilograms, water.After first water dissolves brown sugar, potassium dihydrogen phosphate, then add after megasse, bean cake powder mix and blend, 110 ℃ of sterilizings 1 hour, are cooled to 30 ℃, inoculation compound probiotic nutrient solution, and temperature is controlled at 32 ℃, cultivates 76 hours, obtains composite probiotics ferment liquid.
(4) preparation of compound probiotic powder: get 380 kilograms of 60 object corn flour, 300 kilograms of 60 object bean cake powders, brown sugar double centner, 20 kilograms of composite probiotics ferment liquid, 200 kilograms, water.First, by corn flour, bean cake powder mixing and stirring, then water dissolves brown sugar, 100 ℃ of sterilizings 1 hour, treat that temperature is down to 30 ℃, after adding again composite probiotics ferment liquid to mix, be evenly sprayed on the mixture of corn flour and bean cake powder and stir, make the mixture moisture of corn flour and bean cake powder remain on 29%, temperature is controlled at 33 ℃, aerobic fermentation 5 days, and then temperature is controlled to 28 ℃, anaerobic fermentation 2 days, at 50 ℃ of temperature, dry, pulverize to obtain compound probiotic powder.
(5) produce 1000 kilograms of feed addictives containing 1B compound probiotic, get 20 kilograms, 1B powder, 980 kilograms, compound probiotic powder mixes.
Embodiment 3
(1) preparation of 1B powder: get 4 of corynebacterium glutamicum bacterial classifications, access in 2 kilograms of fluid nutrient mediums of the bacterium of having gone out, shaking table temperature arranges 34 ℃, and rotating speed arranges 1200r/h, cultivates 24h, standby; Get each 5 kilograms of 60 object bean cake powders, megasses, 0.5 kilogram of peptone, 2 kilograms of corynebacterium glutamicum nutrient solutions, 87.5 kilograms, water.After first peptone water being dissolved, add bean cake powder, megasse mix and blend even, in 100 ℃ of sterilizings 30 minutes, be cooled to 30 ℃, access corynebacterium glutamicum nutrient solution expands cultivation again, and temperature is controlled at 32 ℃, aerobic fermentation 72h, obtain 1B liquid, by ion-exchange, extract 1B, through crystallization, the dry 1B powder product that obtains.
(2) preparation of compound probiotic nutrient solution: get 0.06 kilogram of glucose, 0.12 kilogram of sucrose, 0.06 kilogram of bean cake powder, 0.006 kilogram of potassium dihydrogen phosphate, 0.09 kilogram of megasse, each 2 of bacillus subtilis, lactobacillus acidophilus, candida utili inclined-plane bacterium and enterococcus faecalis, 2.664 kilograms, water.First water dissolves glucose, sucrose, potassium dihydrogen phosphate, then add after bean cake powder, megasse mix and blend, part classifies in three categories, 120 ℃ of sterilizings 30 minutes, be cooled to after 30 ℃, access respectively bacillus subtilis, lactobacillus acidophilus, candida utili inclined-plane bacterium and enterococcus faecalis kind, carry out pure culture; 50 ℃ of cultivations of bacillus subtilis 48 hours, 37 ℃ of cultivations of lactobacillus acidophilus 72 hours, 30 ℃ of cultivations of candida utili 48 hours, 40 ℃ of cultivations of enterococcus faecalis 48 hours, after fermentation ends, get 1 kilogram of l kilogram of bacillus subtilis liquid, l kilogram of lactobacillus acidophilus liquid, 1 kilogram of candida utili bacterium liquid, enterococcus faecalis bacterium liquid and mix, be mixed with compound probiotic nutrient solution.
(3) preparation of composite probiotics ferment liquid: get 12 kilograms of megasses, 10 kilograms, brown sugar, 2 kilograms of bean cake powders, 0.2 kilogram of potassium dihydrogen phosphate, 2 kilograms of compound probiotic nutrient solutions, 73.8 kilograms, water.After first water dissolves brown sugar, potassium dihydrogen phosphate, then add after megasse, bean cake powder mix and blend, 100 ℃ of sterilizings 1 hour, are cooled to 30 ℃, inoculation compound probiotic nutrient solution, and temperature is controlled at 34 ℃, cultivates 80 hours, obtains composite probiotics ferment liquid.
(4) preparation of compound probiotic powder: get 380 kilograms of 60 object corn flour, 300 kilograms of 60 object bean cake powders, brown sugar double centner, 20 kilograms of composite probiotics ferment liquid, 200 kilograms, water.First, by corn flour, bean cake powder mixing and stirring, then water dissolves brown sugar, 100 ℃ of sterilizings 1 hour, treat that temperature is down to 30 ℃, after adding again composite probiotics ferment liquid to mix, be evenly sprayed on the mixture of corn flour and bean cake powder and stir, make the mixture moisture of corn flour and bean cake powder remain on 30%, temperature is controlled at 35 ℃, aerobic fermentation 5 days, and then temperature is controlled to 30 ℃, anaerobic fermentation 2 days, at 50 ℃ of temperature, dry, pulverize to obtain compound probiotic powder.
(5) produce 1000 kilograms of feed addictives containing 1B compound probiotic, get 30 kilograms, 1B powder, 970 kilograms, compound probiotic powder mixes.
Test example 1
Select 28 age in days weanling pigs, according to body weight, sex, be divided at random 4 processing, each processes 6 repetitions, and each repeats 6 pigs, male and female balanced proportion.
Table 1 experimental design
| ? | Additive | Concentration | A piglet number |
| Process 1(blank) | - | - | 36 |
| Process 2 | Embodiment 1 | 1% | 36 |
| Process 3 | Embodiment 2 | 2% | 36 |
| Process 4 | Embodiment 3 | 3% | 36 |
Testing regulations:
Test during 28 age in days left and right, is carried out altogether 35 days during from weaned piglet, during 63 age in days, finishes.When on-test, end, weigh piglet body weight, carry out morning on an empty stomach, and off-test is cleaned out feeder for piglet in first 12 hours, and piglet is weighed after hungry 12 hours.
Average daily gain (ADG)=(off-test litter weight-on-test litter weight)/test pig's head number/test number of days
Average day feed consumption (ADFI)=consumption feed/test pig's head number/test number of days
Feedstuff-meat ratio (F/G)=ADFI/ADG
Feed 1B composite bacteria agent capable 35 days of table 2 weanling pig is produced performance statistics
| ? | ADG(kg) | ADFI（kg） | F/G |
| Process 1(blank) | 0.41 | 0.65 | 1.59 |
| Process 2 | 0.48 | 0.60 | 1.25 |
| Process 3 | 0.45 | 0.61 | 1.36 |
| Process 4 | 0.43 | 0.60 | 1.40 |
As can be seen from the above table, the production performance of adding the processed group piglet of 1B compound probiotic is all better than blank group, illustrate that adding 1B compound probiotic can maintain weanling pig intestinal flora balance as additive, promote its digest food, improve the utilization rate of protein, promote animal growth and development, strengthen livestock and poultry physique, improve efficiency of feed utilization.
Claims (6)
1. containing a feed addictive for 1B compound probiotic, it is characterized in that, comprise the component of following weight percentage: 1B powder 1-3%, compound probiotic powder 97-99%.
2. the preparation method of the feed addictive containing 1B compound probiotic according to claim 1, is characterized in that, comprises the steps:
(1) preparation of 1B powder: get corynebacterium glutamicum bacterial strain, be inoculated in fluid nutrient medium, activation, shaking table is cultivated 24h, obtains corynebacterium glutamicum nutrient solution; After water dissolves peptone, add after bean cake powder, megasse mix and blend, sterilizing 1 hour, be cooled to 30 ℃, access corynebacterium glutamicum nutrient solution expands cultivation again, and fermentation 72h, obtains 1B liquid, by ion-exchange, extract 1B, through crystallization, the dry 1B powder that obtains;
(2) preparation of compound probiotic nutrient solution: after water dissolves glucose, sucrose, potassium dihydrogen phosphate, add after bean cake powder, megasse mix and blend, in 120 ℃ of sterilizings 30 minutes, be cooled to 30 ℃, access respectively bacillus subtilis, lactobacillus acidophilus, candida utili bacterium and excrement intestines ball slant strains and carry out pure culture, after cultivation finishes, by equal-volume, mix, be mixed with compound probiotic nutrient solution;
(3) preparation of composite probiotics ferment liquid: water adds after bean cake powder, megasse mix and blend after brown sugar, potassium dihydrogen phosphate are dissolved, and sterilizing 1 hour, is cooled to 30 ℃, and access compound probiotic nutrient solution, obtains composite probiotics ferment liquid;
(4) preparation of compound probiotic powder: by corn flour, bean cake powder mixing and stirring, after water dissolves brown sugar, in 100 ℃ of sterilizings 1 hour, treat that temperature is down to 30 ℃, then after adding composite probiotics ferment liquid to mix, be evenly sprayed on the mixture of corn flour and bean cake powder and stir, make the mixture of corn flour and bean cake powder keep moisture, aerobic fermentation 5 days, and then anaerobic fermentation 2 days are dried and are pulverized to obtain compound probiotic powder at 50 ℃ of temperature;
(5) 1B powder and compound probiotic powder are mixed in proportion to the composite probiotics feed additive of making containing 1B.
3. preparation method according to claim 2, is characterized in that: the described activation temperature of step (1) is 30-34 ℃, and described sterilising temp is 100-120 ℃, and the temperature that described nutrient solution expands cultivation is again controlled at 30-32 ℃; Preferably, described activation temperature is 30-32 ℃, and described sterilising temp is 110-120 ℃, and the temperature that described nutrient solution expands cultivation is again controlled at 30 ℃.
4. preparation method according to claim 2, it is characterized in that: the described pure culture of step (2) is, cultivate 48 hours for bacillus subtilis 45-50 ℃, cultivate 72 hours for lactobacillus acidophilus 35-37 ℃, 25-30 ℃ of cultivation of candida utili bacterium 48 hours, cultivates 48 hours for enterococcus faecalis 35-40 ℃.
5. preparation method according to claim 2, it is characterized in that: the described sterilising temp of step (3) is 100 ℃-120 ℃, the incubation time that described access compound probiotic nutrient solution obtains composite probiotics ferment liquid is 72-80 hour, and it is 30 ℃-35 ℃ that temperature is controlled.
6. preparation method according to claim 2, is characterized in that: the mixture moisture of the described corn flour of step (4) and bean cake powder remains on 28-30%, and temperature is controlled at 30-35 ℃ of aerobic fermentation, and temperature is controlled 25-30 ℃ of anaerobic fermentation.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103960484A (en) * | 2014-05-16 | 2014-08-06 | 四川农业大学 | Compound nutrient for enhancing immunity of ruminant livestock |
| CN103960552A (en) * | 2014-05-16 | 2014-08-06 | 四川农业大学 | Coarse fiber and urea mixed condensation compound for livestock |
| CN110214853A (en) * | 2019-06-21 | 2019-09-10 | 赣州市畜牧研究所 | A kind of method that the fermentation of navel orange slag prepares high-content lysine feed |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101321860A (en) * | 2005-12-08 | 2008-12-10 | Cj第一制糖株式会社 | Fermentation process for preparing L-lysine |
| CN101803681A (en) * | 2009-12-30 | 2010-08-18 | 沈阳科丰牧业科技有限公司 | Oligopolymerization chitosan-astragalus polysaccharide ecological feed additive |
| CN101803679A (en) * | 2009-12-30 | 2010-08-18 | 沈阳科丰牧业科技有限公司 | Micro-ecological feed additive for enhancing color of pet fish and preparation method thereof |
| CN101810249A (en) * | 2010-01-08 | 2010-08-25 | 沈阳科丰牧业科技有限公司 | Method for producing complex-probiotic group by solid fermentation |
| CN102461735A (en) * | 2010-11-15 | 2012-05-23 | 黑龙江省农业科学院畜牧研究所 | Active probiotics feed additive |
-
2013
- 2013-11-29 CN CN201310631023.8A patent/CN103621792A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101321860A (en) * | 2005-12-08 | 2008-12-10 | Cj第一制糖株式会社 | Fermentation process for preparing L-lysine |
| CN101803681A (en) * | 2009-12-30 | 2010-08-18 | 沈阳科丰牧业科技有限公司 | Oligopolymerization chitosan-astragalus polysaccharide ecological feed additive |
| CN101803679A (en) * | 2009-12-30 | 2010-08-18 | 沈阳科丰牧业科技有限公司 | Micro-ecological feed additive for enhancing color of pet fish and preparation method thereof |
| CN101810249A (en) * | 2010-01-08 | 2010-08-25 | 沈阳科丰牧业科技有限公司 | Method for producing complex-probiotic group by solid fermentation |
| CN102461735A (en) * | 2010-11-15 | 2012-05-23 | 黑龙江省农业科学院畜牧研究所 | Active probiotics feed additive |
Non-Patent Citations (1)
| Title |
|---|
| 林文銮等: "福建甘蔗废糖蜜生物合成L-赖氨酸的研究", 《华侨大学学报(自然科学版)》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103960484A (en) * | 2014-05-16 | 2014-08-06 | 四川农业大学 | Compound nutrient for enhancing immunity of ruminant livestock |
| CN103960552A (en) * | 2014-05-16 | 2014-08-06 | 四川农业大学 | Coarse fiber and urea mixed condensation compound for livestock |
| CN110214853A (en) * | 2019-06-21 | 2019-09-10 | 赣州市畜牧研究所 | A kind of method that the fermentation of navel orange slag prepares high-content lysine feed |
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Application publication date: 20140312 |