CN104974967A - Bacillus pumilus strain and composite microecological preparation for nursing baby pigs - Google Patents

Bacillus pumilus strain and composite microecological preparation for nursing baby pigs Download PDF

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Publication number
CN104974967A
CN104974967A CN201510436127.2A CN201510436127A CN104974967A CN 104974967 A CN104974967 A CN 104974967A CN 201510436127 A CN201510436127 A CN 201510436127A CN 104974967 A CN104974967 A CN 104974967A
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bacillus
cctcc
child care
preparation
bacillus pumilus
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CN104974967B (en
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张梁
张君胜
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Jiangnan University
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Jiangnan University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Abstract

The invention provides a Bacillus pumilus ZS008 strain which is collected by China Center for Type Culture Collection, wherein the collection number is CCTCC NO.M2015451, and the collection date is July 13th, 2015. The invention also provides a composite microecological Bacillus preparation for nursing baby pigs, which is prepared from the following strains: Bacillus pumilus CCTCC No.M2015451, Bacillus coagulans CCTCC No.M2015273, Bacillus subtilis CCTCC No.M 2011443 and Bacillus licheniformis CCTCC No.M 2012458. The Bacillus pumilus strain can quickly solve the problems of dysentery, diarrhea and the like in baby pigs, and can enhance the health level of the nursing baby pigs in the long-term usage process.

Description

One bacillus pumilus and child care piglet compound micro-ecological preparation thereof
Technical field
The present invention relates to microbial technology field, especially relate to a bacillus pumilus, and be of value to the application in child care piglet.
Background technology
Child care piglet, be called for short weanling pig, the pig in this stage is quite fragile, and maternal antibody disappears, and new immunity system is not also set up, and adds the reasons such as fast growth, stressors are many, is easy to that various disease occurs and even occurs Large Scale Death.Although microbiotic, pharmaceutical chemicals can solve produced problem, the drug resistance caused thus and drug residue problem within a short period of time, likely can have an impact to piglet late growing stage.How improving child care piglet healthy state, surviving rate, reguarity, give full play to the rear validity of growth, is each raiser's questions of common interest.
Probiotics has regulation and control animal intestinal microbial balance, propagation probiotics, suppresses harmful bacteria, reduces gut pH, improves the effects such as digestive tract enzyme activity, nutritive digestibility and immunity of organisms, increasing about the application report of probiotics in piglet cultivation.CN 101703136B reports the application of plant lactobacillus active bacteria formulation on control baby pig diarrhea, and baby pig diarrhea is efficient more than 90%, curative ratio more than 80%.CN 103255080B reports Bacillus coagulans (Bacillus coagulans HM-09) and is preparing the application improved in the feed of suckling piglet production performance and diarrhoea, the incidence of diarrhea of weaned piglets can be reduced, and then reduce the generation of intestines source property disease, improve feed digestibility, improve pig starter feed utilization ratio.CN 103205373B reports the clostridium butylicum that a strain cultivates for suckling piglet, can in suckling piglet enteron aisle antagonism animal pathogen, maintain and microecosystem regulation balance, enhancing body immunologic function, and produce starch plum, VITAMIN, promote animal digesting and assimilating and trophism feed.
In general, probiotics onset is slow, needs to adhere to using just can receive good effect in livestock and poultry cultivation process.Therefore, develop a kind of can long effectively healthful with, again can the probiotics of the problem such as quick solution baby pig diarrhea, diarrhea, steadily produce seem particularly necessary for realizing child care piglet.
Summary of the invention
For the problems referred to above that prior art exists, the applicant provides a bacillus pumilus and child care piglet compound micro-ecological preparation thereof.The present invention can the problem such as quick solution baby pig diarrhea, diarrhea, can promote again the health level of child care piglet in life-time service process.
Technical scheme of the present invention is as follows:
One bacillus pumilus (Bacillus pumilus) ZS008, is preserved in China typical culture collection center, and deposit number is CCTCC No.M2015451, and preservation date is on July 13rd, 2015.Depositary institution address is China. Wuhan. and Wuhan University.
A kind of child care piglet compound microecological bacillus preparation, is prepared by following strain combination and becomes composite fungus agent: bacillus pumilus CCTCC No.M2015451, Bacillus coagulans CCTCC No.M2015273, subtilis CCTCC No.M 2011443, Bacillus licheniformis CCTCC No.M 2012458.
The living bacteria count of described compound microecological bacillus preparation is 1.4 × 10 8-7.0 × 10 10cfu/g, the moiety of each bacterial classification is as follows:
Described Bacillus coagulans is Bacillus coagulans (Bacillus coagulans) ZS007, is preserved in China typical culture collection center, and deposit number is CCTCC NO:M 2015273.
Described child care piglet is with the application of compound microecological bacillus preparation the production performance promoting child care piglet.Usage quantity is 1.0 ~ 5.0g/ previous day.
Single bacterium powder of subtilis CCTCC No.M 2011443, Bacillus licheniformis CCTCC No.M 2012458 is purchased from Lv Shengyuan bio tech ltd, Taizhou, and its product specification is 5.0 × 10 10cfu/g
The technique effect that the present invention is useful is:
(1) can the problem such as quick solution baby pig diarrhea, diarrhea;
(2) after life-time service, the health level of child care piglet can be promoted, thus reach the effect promoting child care piglet throughput.
Embodiment
1, the screening of bacillus pumilus
2g is taken from the pedotheque near great pig farm, jiangsu wuxi side, add 98ml stroke-physiological saline solution, after 80 DEG C of thermal treatment 10min, 37 DEG C of shaking tables vibrate under 120rpm condition 20min, Aspirate supernatant 1ml add dilution spread in LB substratum and carry out strains separation.The genus kind of bacterial classification effectively and is accurately determined by carrying out the means such as morphological observation, bio-chemical characteristics, 16S rDNA analysis to bacterial strain.
Finally obtain the bacterial classification being numbered ZS008, be accredited as bacillus pumilus (Bacillus pumilus).This culture presevation is in China typical culture collection center, and deposit number is CCTCC No.M2015451, and preservation date is on July 13rd, 2015.
2, the screening of Bacillus coagulans
2g is taken from the pedotheque near the dairy cow farm of jiangsu wuxi Mashan, add 98ml stroke-physiological saline solution, after 80 DEG C of thermal treatment 10min, 37 DEG C of shaking tables vibrate under 120rpm condition 20min, Aspirate supernatant 1ml add dilution spread in LB substratum and carry out strains separation.The genus kind of bacterial classification effectively and is accurately determined by carrying out the means such as morphological observation, bio-chemical characteristics, 16S rDNA analysis to bacterial strain.
Finally obtain the bacterial classification being numbered ZS-007, be accredited as Bacillus coagulans (Bacillus coagulans).
ZS-007 is preserved in China typical culture collection center on May 3rd, 2015, and deposit number is CCTCC M 2015273.
3, the preparation of compound micro-ecological preparation
Compound micro-ecological preparation by bacillus pumilus CCTCC No.M2015451, Bacillus coagulans CCTCC No.M 2015273, subtilis CCTCC No.M 2011443, Bacillus licheniformis CCTCC No.M 2012458 is composite forms.
Wherein single bacterium powder buying of subtilis CCTCC No.M 2011443, Bacillus licheniformis CCTCC No.M 2012458 is from Lv Shengyuan bio tech ltd, Taizhou, and its product specification is 5.0 × 10 10cfu/g.
The probiotics product that embodiment 1 ~ 3 is prepared, each content of microorganisms is as following table 1:
Table 1
Single bacterium powder is according to after both certainty ratio mixes, and add dextrose anhydrous as carrier, add to gross weight 100% (using genus bacillus as effective constituent, makes product gross weight meet final viable count and reaches 4.0 × 10 after adding carrier 8cfu/g, mixes rear for subsequent use.
The course of processing should ensure the clean of operating environment, avoids polluting.Control environment humidity less than 70%, and each moisture content of raw material is lower than 13%, and this product is preserved 24 months under making rear normal temperature, and at-18 DEG C, the storage life can reach more than 60 months, and in the quality guaranteed period, viable count is stablized.
4, the effect of compound probiotic agent on child care piglet
Select from numerous autotrophy with Du × length criticized × Dasanyuan hybridization weanling pig 90.According to parity, body weight, the same or analogous principle of male and female ratio, piggy is divided into A, B, C3 group, wherein A group is control group, and B test group 1, C group is test group 2, and often group establishes 2 repetitions, each repetition 15, random packet, separately numbering; Each group of basal diet identical (honest complete diet pellet), trophic level is consistent.A group does not add any zymin, the basis of B group basal diet is added the compound micro-ecological preparation (embodiment 1) containing bacillus pumilus, and C group is not with B group but containing bacillus pumilus; The consumption that probiotics consumption is is the 1.0g/ previous day.Piglet 21 age in days, mean body weight 6.5kg weans, preliminary experiment 7 days, feeding experiment 30 days.Raise Nanjing, place cultivation cooperative society.
Test-results is as shown in table 2 ~ 4.
Table 2 tests pig weightening finish and average daily gain in trial period
Table 3 enzyme preparation and protics of feeding tests each group of price of deed situation
Table 4 enzyme preparation and protics of feeding tests the frequency of each group of grice diarrhoea
Piglet has loose bowels after phenomenon, uses probiotics product to treat by the deal of every pig 5g.After product stirs with cold boiling water 10mL, suction syringe, and gavage along piglet bicker with syringe.Time in probiotics product containing bacillus pumilus, after piglet 4-6hr, spirit takes a turn for the better, termination of having loose bowels in 24hr, recovers appetite.Time in probiotics product not containing bacillus pumilus, after piglet 12hr, spirit takes a turn for the better; Once, termination of having loose bowels for about 2 days, recovers appetite in after treatment.

Claims (6)

1. a bacillus pumilus (Bacillus pumilus) ZS008, is preserved in China typical culture collection center, and deposit number is CCTCC NO.M2015451, and preservation date is on July 13rd, 2015.
2. a child care piglet compound microecological bacillus preparation, is characterized in that being prepared by following strain combination becoming composite fungus agent: bacillus pumilus CCTCC NO.M2015451, Bacillus coagulans CCTCCNO.M2015273, subtilis CCTCC NO.M 2011443, Bacillus licheniformis CCTCCNO.M 2012458.
3. child care piglet compound microecological bacillus preparation according to claim 2, is characterized in that the living bacteria count of described compound microecological bacillus preparation is 1.4 × 10 8-7.0 × 10 10cfu/g, the moiety of each bacterial classification is as follows:
4. child care piglet compound microecological bacillus preparation according to claim 2, it is characterized in that described Bacillus coagulans is Bacillus coagulans (Bacillus coagulans) ZS007, be preserved in China typical culture collection center, deposit number is CCTCC NO:M 2015273.
5. the child care piglet according to claim 2 application of compound microecological bacillus preparation, is characterized in that the production performance promoting child care piglet.
6. the child care piglet according to claim 5 application of compound microecological bacillus preparation, is characterized in that the usage quantity of described compound microecological bacillus preparation is 1.0 ~ 5.0g/ previous day.
CN201510436127.2A 2015-07-22 2015-07-22 One bacillus pumilus and its child care piglet compound micro-ecological preparation Active CN104974967B (en)

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PCT/CN2016/090899 WO2017012573A1 (en) 2015-07-22 2016-07-21 Bacillus pumilus strain and microbial ecological combined agent thereof for weaned piglet
AU2016297405A AU2016297405B2 (en) 2015-07-22 2016-07-21 Bacillus pumilus strain and microbial ecological combined agent thereof for weaned piglet

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017012573A1 (en) * 2015-07-22 2017-01-26 江南大学 Bacillus pumilus strain and microbial ecological combined agent thereof for weaned piglet
TWI569731B (en) * 2015-12-30 2017-02-11 聯發生物科技股份有限公司 Bacterial additive for animal food and producing method thereof
CN106922951A (en) * 2015-12-30 2017-07-07 联发生物科技股份有限公司 Feed addition microbial inoculum and preparation method thereof, bacterial strain screening method
CN111548965A (en) * 2020-05-20 2020-08-18 山东省农业科学院生物技术研究中心 Donkey-derived bacillus pumilus and application thereof in preparation of medicine for treating diarrhea of donkey colt
CN111801020A (en) * 2018-02-02 2020-10-20 诺维信公司 Management of pathogenic Lawsonia

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114272552A (en) * 2021-12-06 2022-04-05 紫茎泽兰科技(云南)有限公司 Method for detoxifying Eupatorium adenophorum

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CN102505003A (en) * 2011-12-19 2012-06-20 湖南省微生物研究所 High-density fermenting method of bacillus coagulans for livestock and poultry, as well as preparation prepared by method and application thereof
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CN104171756A (en) * 2014-09-02 2014-12-03 双胞胎（集团）股份有限公司 Conservation pig feed used on family pig farm and feeding mode of conservation pig feed
CN104263683A (en) * 2014-09-15 2015-01-07 中国农业科学院饲料研究所 Bacillus pumilus 315 with probiotic effect and application thereof

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CN103255080B (en) * 2013-03-22 2015-05-27 内蒙古和美科盛生物技术有限公司 Excellent bacillus coagulans for breeding piglets and application thereof
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CN104472862A (en) * 2014-12-29 2015-04-01 江苏千药堂国医研究院有限公司 Biological feed additive and preparation method thereof
CN104974967B (en) * 2015-07-22 2018-04-17 江南大学 One bacillus pumilus and its child care piglet compound micro-ecological preparation
CN105341451A (en) * 2015-10-26 2016-02-24 滁州市南谯区施集镇兴牧生态养殖场 Special feed capable of promoting digestion and preventing diarrhoea for weaned piggies

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CN101669576A (en) * 2008-09-12 2010-03-17 安琪酵母股份有限公司 Yeast composition and feed
WO2013029013A1 (en) * 2011-08-24 2013-02-28 Dupont Nutrition Biosciences Aps Enzyme producing bacillus strains
CN102505003A (en) * 2011-12-19 2012-06-20 湖南省微生物研究所 High-density fermenting method of bacillus coagulans for livestock and poultry, as well as preparation prepared by method and application thereof
CN104171756A (en) * 2014-09-02 2014-12-03 双胞胎（集团）股份有限公司 Conservation pig feed used on family pig farm and feeding mode of conservation pig feed
CN104263683A (en) * 2014-09-15 2015-01-07 中国农业科学院饲料研究所 Bacillus pumilus 315 with probiotic effect and application thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017012573A1 (en) * 2015-07-22 2017-01-26 江南大学 Bacillus pumilus strain and microbial ecological combined agent thereof for weaned piglet
TWI569731B (en) * 2015-12-30 2017-02-11 聯發生物科技股份有限公司 Bacterial additive for animal food and producing method thereof
CN106922951A (en) * 2015-12-30 2017-07-07 联发生物科技股份有限公司 Feed addition microbial inoculum and preparation method thereof, bacterial strain screening method
CN111801020A (en) * 2018-02-02 2020-10-20 诺维信公司 Management of pathogenic Lawsonia
CN111548965A (en) * 2020-05-20 2020-08-18 山东省农业科学院生物技术研究中心 Donkey-derived bacillus pumilus and application thereof in preparation of medicine for treating diarrhea of donkey colt
CN111548965B (en) * 2020-05-20 2022-03-18 山东省农业科学院 Donkey-derived bacillus pumilus and application thereof in preparation of medicine for treating diarrhea of donkey colt

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WO2017012573A1 (en) 2017-01-26
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