CN107007633A - A kind of probiotics polysaccharide composite formulation preparation method - Google Patents
A kind of probiotics polysaccharide composite formulation preparation method Download PDFInfo
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- CN107007633A CN107007633A CN201610064248.3A CN201610064248A CN107007633A CN 107007633 A CN107007633 A CN 107007633A CN 201610064248 A CN201610064248 A CN 201610064248A CN 107007633 A CN107007633 A CN 107007633A
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- 239000006041 probiotic Substances 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 36
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 36
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 32
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 32
- 239000000203 mixture Substances 0.000 title claims abstract description 17
- 238000009472 formulation Methods 0.000 title claims abstract description 15
- 239000002131 composite material Substances 0.000 title claims abstract description 13
- 150000004676 glycans Chemical class 0.000 title abstract 7
- 235000006533 astragalus Nutrition 0.000 claims abstract description 20
- 239000000843 powder Substances 0.000 claims abstract description 19
- 241001061264 Astragalus Species 0.000 claims abstract description 18
- 150000001875 compounds Chemical class 0.000 claims abstract description 18
- 210000004233 talus Anatomy 0.000 claims abstract description 18
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 12
- 229920001491 Lentinan Polymers 0.000 claims abstract description 12
- 229940115286 lentinan Drugs 0.000 claims abstract description 12
- 239000003094 microcapsule Substances 0.000 claims abstract description 12
- 230000000529 probiotic effect Effects 0.000 claims abstract description 10
- 241000193755 Bacillus cereus Species 0.000 claims abstract description 8
- 241000726221 Gemma Species 0.000 claims abstract description 8
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 7
- 241000186660 Lactobacillus Species 0.000 claims abstract description 7
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 7
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 6
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 5
- 239000002775 capsule Substances 0.000 claims abstract description 4
- 241001037822 Bacillus bacterium Species 0.000 claims abstract description 3
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 3
- 239000000047 product Substances 0.000 claims description 25
- 150000004804 polysaccharides Chemical class 0.000 claims description 19
- 241000894006 Bacteria Species 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 239000012043 crude product Substances 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 238000013329 compounding Methods 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 241000045403 Astragalus propinquus Species 0.000 claims description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- 239000000783 alginic acid Substances 0.000 claims description 2
- 229920000615 alginic acid Polymers 0.000 claims description 2
- 229960001126 alginic acid Drugs 0.000 claims description 2
- 235000010443 alginic acid Nutrition 0.000 claims description 2
- 150000004781 alginic acids Chemical class 0.000 claims description 2
- 229940107666 astragalus root Drugs 0.000 claims description 2
- 239000011575 calcium Substances 0.000 claims description 2
- 229910052791 calcium Inorganic materials 0.000 claims description 2
- 239000001110 calcium chloride Substances 0.000 claims description 2
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- 238000005119 centrifugation Methods 0.000 claims description 2
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- 238000001914 filtration Methods 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 238000002386 leaching Methods 0.000 claims description 2
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- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 238000001694 spray drying Methods 0.000 claims description 2
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- 238000003756 stirring Methods 0.000 claims description 2
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- 238000005660 chlorination reaction Methods 0.000 claims 1
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- 206010059866 Drug resistance Diseases 0.000 abstract description 4
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- 238000011160 research Methods 0.000 description 3
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- 206010012735 Diarrhoea Diseases 0.000 description 2
- 239000009636 Huang Qi Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
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- 241001148471 unidentified anaerobic bacterium Species 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241000304886 Bacilli Species 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
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- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000003124 biologic agent Substances 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 230000002301 combined effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 208000032625 disorder of ear Diseases 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000002434 immunopotentiative effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000009018 li Nutrition 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- -1 polysaccharide compound Chemical class 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Zoology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention relates to a kind of probiotics polysaccharide composite formulation preparation method, using modern biotechnology, probiotics preparation is prepared, mixes produce by a certain percentage with astragalus polyose, lentinan afterwards.Specifically include：1st, prepared by compound probiotic.Pig source lactobacillus capsule, bacillus subtilis, waxy gemma bar bacillus bacterium powder are prepared first, Bacillus acidi lactici microcapsules, bacillus subtilis bacterium powder and bacillus cereus bacterium powder are mixed according to the ratio of mass ratio 3: 1: 1 with appropriate calcium carbonate, compound probiotic product is made.2nd, prepared by complex polysaccharide.Astragalus polyose and lentinan are extracted, astragalus polyose and lentinan root are mixed according to the ratio of mass ratio 1: 1, complex polysaccharide is produced.3rd, probiotics polysaccharide composite preparation is prepared.Compound probiotic product and complex polysaccharide are mixed according to the ratio of mass ratio 3: 1, probiotics polysaccharide composite preparation is obtained.The present invention is pure natural, pollution-free, have no drug resistance.
Description
Technical field
The invention belongs to veterinary medicine, in particular it is a kind of enhancing pigling immunity, adjusts in piglet body
Beneficial bacteria growing, a kind of probiotics-polysaccharide composite preparation for promoting piglet development and preparation method thereof.
Background technology
Probiotics widely uses in China with Chinese medicine individually as green feed additive.Probiotics is not
Only there is regulation animal itself microecological balance, increase internal profitable strain quantity, suppress or kill harmful bacteria,
Strengthen immunity of organisms, preserve the ecological environment wait effect, but also with promote animal digestion, improve produce
Performance, enhancing immunity of organism and premunition, improve the effect such as environmental pollution.Chinese medicine contains multiple nutritional components
And bioactive substance, the immunologic function of body can not only be strengthened, the digestive utilization ratio of nutriment is improved,
And there is the features such as antibacterial, antiviral, Small side effects and noresidue.But in clinical practice, probiotics
Due to poor environment resistance it is poor, the factors such as viable bacteria content in difficulty, and product is relatively low are colonized in enteron aisle
And have impact on the effect of product；Chinese medicine then is difficult to be absorbed because drug effect plays slow, some active ingredients,
The problems such as beneficial bacteria growing may be suppressed and limit its popularization in practice.
Research shows：There is certain synergy between probiotics and Chinese medicine, during one side probiotics can promote
The absorption and utilization of herbal medicine, many Chinese herbal medicines are not effective medicine in itself, but as the precursor of medicine,
After by oral administration, contacted before absorbing with the digested enzyme of alimentary canal, the microorganism species then through alimentary canal bottom add
Could be by enteron aisle selective absorbing after work, transformation；Another aspect chemical composition of Chinese materia medica is complicated, wherein containing
Alkaloid, glucoside, volatile oil and tannin etc. there is therapeutic action, can be that the propagation of probiotics be removed the obstacles；
Grease, resin, carbohydrate, protein and pigment etc. mainly play trophism, and what is had is even inherently prebiotic
The constituent of bacterium cell, can promote proliferation of probiotics.
Li Bing, Wang Linyu et al. research show：The Radix Astragali is as immunopotentiator and antiviral agent, in animal husbandry
Clinic has good using effect.Liu Yang, Japanese plum canopy et al. research demonstrate Chinese herbal medicine astragalus active ingredient pair
Probiotics has growth-promoting functions, and astragalus polyose probiotics symphysis unit can be remarkably reinforced Immune Profile In Chicks function.
The content of the invention
The present invention is by compound probiotic (pig source lactobacillus microcapsules, bacillus subtilis bacterium powder and yeast bacterium powder)
Mixed by a certain percentage with the astragalus polyose using plant polyose as representative and the lentinan using fungi polysaccharide as representative
Compounding is closed, pure natural, no drug residue, pollution-free, the green veterinary medicine that has no drug resistance, have no toxic side effect is extracted
Product-probiotics polysaccharide composite biological agent.
The cardinal principle and technical scheme of the present invention：A kind of probiotics polysaccharide composite formulation preparation method, existing
On the basis of being combined for molecular immunology and traditional Chinese medicine, using modern biotechnology, probiotics preparation is prepared,
Mix produce by a certain percentage with astragalus polyose, lentinan afterwards.Preparation method comprises the following steps：
(1) prepared by compound probiotic.
1. pig source lactobacillus capsule is prepared.
The pig source lactobacillus liquid for cultivating 20h is taken into bacterium mud after 4000r/min centrifugations 20min；With alginic acid
Sodium is coating wall material, using calcium chloride as solidify liquid, and both are respectively in 121 DEG C of sterilizing 20min；It will cool down
After wall material solution mixes 30 minutes uniformly with bacterium solution in two-way constant temperature blender with magnetic force, chlorine is injected with syringe
Change calcium solution, microcapsule granule is collected through 350r/min low-speed centrifugals 10min, taken out after washing 3 times
A small amount of sample determines embedding efficiency and embedding yield；Remaining product is placed in -20 DEG C of pre-freeze 2h, then carries out
Freeze-drying process.
Embedding efficiency/%=(viable count in 1- surface of microcapsule viable count/product) × 100%
Surface of microcapsule viable count is determined：Directly with brine microcapsules top layer thalline, viable bacteria is carried out
Count.
The measure of viable count in product：Product is dissolved in the broken of 0.1mol/L phosphate buffers (PH=7.0)
In wall material, count plate is carried out until completely dissolved.
Embed viable count × 100% of viable count/addition in yield/%=products.
2. bacillus subtilis and the preparation of waxy gemma bar bacillus bacterium powder
By culture 18h bacillus subtilis seed liquor, bacillus cereus seed liquor respectively with 4% (volume
Fraction) 40L liquid fermentation mediums are inoculated in, fermented and cultured, condition of culture are carried out in 50L fermentation tanks
For：1.5% (volume fraction) defoamer is added, rotating speed is 350r/min, and throughput is 10L/min,
Initial p h7.0,35 DEG C of temperature；Sampled 1 time every 2h, the production gemma rate in determination sample, when production gemma
Rate can stop fermentation when reaching more than 90%；The tunning of acquisition is spray-dried, is spray-dried
The inlet temperature of device is set to 146 DEG C, and outlet temperature is set to 110 DEG C；Spray drying measures product after terminating
Quality, and utilize the viable count (cfu/g) in dilution-plate method analytical unit quality product.
3. the compounding of compound probiotic product
By Bacillus acidi lactici microcapsules, bacillus subtilis bacterium powder and bacillus cereus bacterium powder according to mass ratio
3: 1: 1 ratios are mixed with appropriate calcium carbonate, it is ensured that and the total bacteria in product is approximately equal to 6 ×
1010cfu/g。
(2) prepared by complex polysaccharide
1. the extraction of astragalus polyose.Astragalus root is crushed using micronizer, 300 mesh sieve tulles is crossed and is prepared into
In astragalus membranaceus powder, the distilled water for adding 5-8 times, 1-3h is leached at a temperature of 80 DEG C -90 DEG C, filtering to leach
Liquid, leaching liquid is placed after room temperature, under appropriate speed stirring, in 95% alcohol for immersing 2 times of volumes,
Astragalus polyose is precipitated out, and uses 95% ethanol wash, is dried, is drawn astragalus polyose crude product.
2. lentinan is extracted.Extracted, purified according to the step of 1. after mushroom is crushed.
3. complex polysaccharide product configuration.Astragalus polyose and lentinan root are mixed according to the ratio of mass ratio 1: 1.
(3) probiotics polysaccharide composite preparation is prepared
Compound probiotic product and complex polysaccharide are mixed according to the ratio of mass ratio 3: 1, probiotics-polysaccharide is obtained
Compound formulation.
Lactobacillus capsule was being grown in view of lactic acid bacteria is generally anaerobic bacteria or facultative anaerobic bacteria in the present invention
Cheng Zhong, does not form gemma, and resistance is poor, and most acid resistances are poor, and microencapsulation coating is carried out to Bacillus acidi lactici
Afterwards, the resistance of Bacillus acidi lactici can be improved.
The compound formulation application method of the present invention：, can be postnatal 24 hours in newborn piglet in terms of prevention
It is interior, 1.0-1.5 grams of this product is gavaged before colostrum, relatively gently, substantially the piglet of morbid state can fill for weak, body weight
1.5-2.5 grams/head is taken, piglet maternal antibody level, the general level of the health and the digestion power of piglet can be improved；
This preparation 0.3-0.4% is added in piglet premix, pigling immunity can be significantly improved, promotes piglet life
It is long, feedstuff-meat ratio is reduced, the generation of various diseases is reduced；In piglet immunological aftosa, swine fever, highly pathogenic
3-5 days oral 2.5-3 grams of this product/heads, are used in conjunction 3 days, can significantly improve piglet before the epidemic diseases such as pig blue-ear disease
Immune antiboidy level；Treat piglet and occur enteron aisle diarrhoea, can be taken orally in 1-2 week old 2-2.5 grams/time,
3-4 week old 2.5-4.5 grams/time, 5 5-8 grams/of week old above piglets time, can per average daily 3 times
To be fed in compound, also dissolve in cold water and gavage, increase and decrease dose is required according to animal doctor.This product dilutes
After should take as early as possible, can not be heated at high temperature and use, closed, shady place is preserved, storage life 12-18 months.
Through a large amount of it was verified that using this preparation before piglet colostrum, 1-2 can be made using compound formulation of the present invention
Week old cub survival rate brings up to more than 96%；This product can make cub nursing period as piglet premix additive
Foreshorten to 25-28 days, make weanling pig increased weight 15-25%, weanling pig survival rate bring up to 90% with
On；This preparation is used before piglet immunological, 30 days after being immunized, detection aftosa, swine fever, highly pathogenic blue
Otopathy mean antibody levels, its qualification rate improves 15%-20%.The cure rate that this preparation is suffered from diarrhoea to intestine of young pigs
It can reach more than 85%.In addition, farrowing sow can significantly improve the maternal antibody level of piglet using this preparation
And the general level of the health.
Main advantages of the present invention and effect：
1st, compound formulation of the invention directly can be taken for piglet, and probiotics preparation and polysaccharide formulation all pass through
Purity is very high after extraction purification, is easy to accurately calculate piglet usage amount；Probiotics preparation can increase body
Interior beneficial bacterium quantity, suppresses or kills harmful bacteria, promote animal digestion to improve production performance, strengthen body
Immunity, overcomes the drug resistance of conventional antibiotic and chemical synthetic drug antimicrobial；Polysaccharide it is disease-resistant
Toxic action, can resist invasion and attack of each viroid to animal body, it is to avoid antiviral chemicals etc. is violated
About use of the product in Animal husbandry production,
2nd, the present invention is by a large amount of clinical tests, screening study probiotics preparation, effective group of polysaccharide formulation
Divide and optimal prescription, herbal control, Tiny ecosystem preventing and treating and the immunity of organisms combination of enhancing piglet are existed
Together, probiotics preparation can promote the absorption and utilization of the Radix Astragali and mushroom complex polysaccharide first；Polysaccharide composite
Preparation has growth-promoting functions as immunopotentiator and antiviral agent to probiotics；In compound preparation of the present invention,
Probiotics and complex polysaccharide compound formulation can produce the combined effect of complexity, it will be apparent that enhancing piglet immunological work(
Can, weaned piglets are improved, and overcome the above two respective shortcomings and drawback.
3rd, probiotics-polysaccharide of the invention meets the base that preparation is combined in modern molecular immunology and traditional Chinese medicine
On plinth, using modern biotechnology and high-new physics and chemistry process technology, a kind of collection immunopotentiation refined,
Chinese herb prevention and the pure natural, no drug residue of microecology one, it is pollution-free, have no drug resistance, have no side effect
Novel green veterinary drug preparation.
Brief description of the drawings
Fig. 1 makes a living galactopoiesis acidfast bacilli microcapsules flow chart；
Fig. 2 is production bacillus subtilis bacterium powder flow chart；
Fig. 3 is production bacillus cereus bacterium powder flow chart；
Fig. 4 is production astragalus polyose flow chart；
Fig. 5 is production lentinan flow chart.
Embodiment
With reference to embodiment, the invention will be further described.
Embodiment 1：This example is that invention prescription is applied into newborn piglet.
(1) the compound formulation formula of this example：
600 grams of Bacillus acidi lactici microcapsules
200 grams of bacillus subtilis bacterium powder
200 grams of bacillus cereus bacterium powder
160 grams of astragalus polyose crude product
160 grams of lentinan crude product
(2) preparation flow is as follows：
1. produce Bacillus acidi lactici microcapsules according to Fig. 1 flows；
2. produce bacillus subtilis bacterium powder according to Fig. 2 flows；
3. produce bacillus cereus bacterium powder according to Fig. 3 flows；
4. produce astragalus polyose crude product according to Fig. 4 flows；
5. produce lentinan crude product according to Fig. 5 flows.
6. pressing in (one) takes respective mass mixing uniform.
(3) using effect of probiotics-polysaccharide composite preparation
1. effect and purposes
This formulation application can improve piglet maternal antibody level, the general level of the health and the digestible energy of piglet in newborn piglet
Power.
2. usage and dosage
Every newborn piglet uses 1.0-1.5 grams, and for weak, body weight, relatively gently, substantially the piglet of morbid state can gavage
1.5-2.5 grams/head, every gram is diluted with 5-8 milliliters of cold water or triumph salt solution, is shaken up, is sucked the breast after piglet birth
It is preceding to gavage immediately.
3. prevention effect
1. protected using the age in days aftosa of this preparation newborn piglet 7, highly pathogenic PRRS, swine fever maternal antibody
Level 8%-11% higher than control group.Refer to following table
Note：Using this preparation and blank control group, sow feeding environment, immune state, trophic level are completely the same；
It is completely the same using this preparation and blank control group feeding piglet environment, trophic level；Use this preparation and sky
The white class epidemic disease antibody detection method of control group three is completely the same.
Claims (1)
1. a kind of probiotics polysaccharide composite formulation preparation method, it is characterised in that in modern molecular immunology and the traditional Chinese medical science
On the basis of be combined, produced using modern biotechnology, preparation method comprises the following steps：
It is prepared by I, compound probiotic：
1. pig source lactobacillus capsule is prepared
The pig source lactobacillus liquid for cultivating 20h is taken into bacterium mud after 4000r/min centrifugations 20min；With alginic acid
Sodium is coating wall material, using calcium chloride as solidify liquid, and both are respectively in 121 DEG C of sterilizing 20min；Wall will be cooled down
After material solution mixes 30 minutes uniformly with bacterium solution in two-way constant temperature blender with magnetic force, chlorination is injected with syringe
Calcium solution, microcapsule granule is collected through 350r/min low-speed centrifugals 10min, takes out a small amount of after washing 3 times
Sample determines embedding efficiency and embedding yield；Remaining product is placed in -20 DEG C of pre-freeze 2h, is then freezed
Drying process；
2. bacillus subtilis and the preparation of waxy gemma bar bacillus bacterium powder
By culture 18h bacillus subtilis seed liquor, bacillus cereus seed liquor respectively with volume fraction
4% is inoculated in 40L liquid fermentation mediums, and fermented and cultured is carried out in 50L fermentation tanks, and condition of culture is：
The defoamer of volume fraction 1.5% is added, rotating speed is 350r/min, throughput is 10L/min, initial p h7.0,
35 DEG C of temperature；Every 2h sample 1 time, the production gemma rate in determination sample, when production gemma rate reach 90% with
Fermentation can be stopped when upper；The tunning of acquisition is spray-dried, the inlet temperature of spray dryer
146 DEG C are set to, outlet temperature is set to 110 DEG C；Spray drying measures product quality after terminating, and utilizes
Viable count in dilution-plate method analytical unit quality product；
3. the compounding of compound probiotic product
By Bacillus acidi lactici microcapsules, bacillus subtilis bacterium powder and bacillus cereus bacterium powder according to mass ratio
3: 1: 1 ratios are mixed with appropriate calcium carbonate, it is ensured that the total bacteria in product is approximately equal to 6 × 1010cfu/g；
It is prepared by II, complex polysaccharide
1. the extraction of astragalus polyose, astragalus root is crushed using micronizer, is crossed 300 mesh sieve tulles and is prepared into
In astragalus membranaceus powder, the distilled water for adding 5-8 times, 1-3h is leached at a temperature of 80 DEG C -90 DEG C, filtering to leach
Liquid, leaching liquid is placed after room temperature, yellow in 95% alcohol for immersing 2 times of volumes under appropriate speed stirring
Astragalus polysaccharides are precipitated out, and use 95% ethanol wash, are dried, are drawn astragalus polyose crude product；
2. lentinan is extracted, and is extracted, purified according to the step of 1. after mushroom is crushed；
3. complex polysaccharide product configuration, astragalus polyose and lentinan root are mixed according to the ratio of mass ratio 1: 1；
III, probiotics polysaccharide composite preparation are prepared
Compound probiotic product and complex polysaccharide are mixed according to the ratio of mass ratio 3: 1, probiotics-polysaccharide is obtained
Compound formulation.
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Application publication date: 20170804 |