CN106615629A - Bee larva probiotic oral liquid for animal healthcare - Google Patents
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- CN106615629A CN106615629A CN201611205874.6A CN201611205874A CN106615629A CN 106615629 A CN106615629 A CN 106615629A CN 201611205874 A CN201611205874 A CN 201611205874A CN 106615629 A CN106615629 A CN 106615629A
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- 230000000529 probiotic effect Effects 0.000 title abstract description 4
- 238000000855 fermentation Methods 0.000 claims abstract description 20
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 26
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- 235000014655 lactic acid Nutrition 0.000 claims description 13
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- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 9
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- 238000002360 preparation method Methods 0.000 claims description 6
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/515—Animalis
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A bee larva probiotic oral liquid for animal healthcare is prepared by fermenting bee larva homogenate pretreated with animal probiotics disclosed in Lists of Feed Additives by Department of Agriculture, the original nutritional composition and functional active substances in bee honey are retained, and the bee larva proteins are fermented by animal probiotics and hydrolyzed into small-molecular bioactive peptide so that the health-care function of the oral liquid is enhanced. In addition, rich flavor matters are produced during fermentation, the flavor is improved, and good attracting effect is provided. Animal experiments prove that the bee larva probiotic oral liquid for animal healthcare is capable of lowering the chances of piglet diarrhea significantly and decreasing feed-gain ratio and input-gain ratio significantly, and average daily weight increase is increased.
Description
Technical field
The invention discloses a kind of animal health queen bee tire probio oral liquid, belongs to feed addictive and animal-breeding
Field.
Background technology
Queen bee tire, also known as queen bee nit, is that the embryonated egg Jing workers bee of honeybee are fed with the larva that new fresh royal jelly grows
Body.Nutritional studies show that queen bee tire is rich in protein, amino acid, chitin polysaccharide, vitamin, trace element, enzyme, nucleic acid, Huang
Ketone etc., nutritive value is high.Bioactivity research shows that queen bee mold has suppression or delaying cell aging, enhancing organism to exempt from
The health-care efficacies such as epidemic disease function.Therefore, it is a kind of natural health resource of worth Devoting Major Efforts To Developing.
China is time-honored bee-keeping big country, and bee colony number and bee product yield are world prostatitis, have possessed 800 at present
Many ten thousand groups of honeybees, queen bee tire annual production is up to thousands of tons of.But used as the byproduct of production royal jelly, the whole nation is annual to be processed profit
Queen bee tire only has 50 tons or so, causes the significant wastage of resource.In food processing field, minority is had at present to queen bee tire
Developmental research, such as University Of Agriculture and Forestry In Fujian Liu Xiu is beautiful and Zhu Haibo is also carried out to the preparation of queen bee fetus oral liquid and buccal tablet
Corresponding research.Domestic patent ZL93100883.2 and CN01107658.5 disclose respectively the related invention of queen bee foetus wine.And incite somebody to action
Queen bee tire is used for field of fodder, there is not yet relevant report.
The invention discloses a kind of animal health queen bee tire probio oral liquid based on the queen bee tire that ferments.Queen bee tire
After being fermented, protein is broken down into small-molecular peptides with amino acid, is more beneficial for human consumption's absorption, and overcomes queen bee tire
The problem of the easy thermal denaturation of protein.Importantly, fermentation energy makes the acid amides bond fission of anaphylactogen, its molecular mass is reduced,
And then reduction anaphylaxis；Fermentation can also change the tertiary structure of allergen epitope so as to be blanked or eliminate so as to reduce sensitization
Property.Additionally, queen bee tire probio oral liquid also has two important functions to ensure intestine of young pigs health, reduce diarrhoea：1）Wherein
Bacillus in animal body especially enteron aisle back segment can produce a large amount of endogenous enzymes, enteron aisle leading portion can not digested completely
Nutrient fully carries out secondary digestion, to improve digesting and assimilating for nutrient, it is to avoid intestines back segments（Such as colon, rectum）Eutrophy
Change, it is to avoid the breeding of harmful bacteria here produces toxin, affects body health；2）Peptides antibacterial is produced after the hydrolysis of queen bee fetoprotein
Matter, can directly kill such as C.perfringens harmful bacteria, it is ensured that intestinal health.
For health care of the animal probio to animal, academic circles at present is reached common understanding.Research shows that animal is prebiotic
Bacterium is entered after animal intestinal, on the one hand can promote the propagation of intrinsic beneficial bacterium in enteron aisle, on the other hand can effectively suppress grape
The pathogenic bacteria such as coccus, candida albicans, Pseudomonas aeruginosa, Escherichia coli, shigella dysenteriae and Salmonella typhi and the breeding of spoilage organisms, so as to
Ensure animal intestinal health.Therefore, people develop a series of treatments or prevent all kinds of animal probiotics preparations of intestines problem.
But have not yet to see the research and development of the animal probiotic products with queen bee fetoprotein as fermentation substrate.
The content of the invention
Its health-care effect and biological characteristics are waved for fully exploitation queen bee tire resource concurrence, the present invention provides a kind of animal and protects
Strong to use queen bee tire probio oral liquid, its preparation process is as follows：
1 adds lactose in queen bee tire homogenate, and pasteurization is carried out after mixing.Wherein Inclusion of Lactose is in mass ratio：Honeybee
King's tire homogenate:Lactose=100:1~100:2；Pasteurisation parameter is 55-60 DEG C, minute time 25-30.
2 per milliliter of pretreated queen bee tire homogenates add animal probio based on total plate count 105~107 CFU.It is dynamic
Thing probio includes bacillus（It is aerobic）The bacillus licheniformis and bacillus subtilis of class, and lactic acid bacteria（Anaerobism）Class
Enterococcus faecalis, lactoenterococcus, lactobacillus acidophilus, Lactobacillus plantarum, animal bifidobacteria.Fermented bacterium is above-mentioned bud in use
At least include a kind of bacillus and a kind of lactic acid bacteria in spore bacillus and the combination of lactic acid bacteria, i.e. fermented bacterium.
3 carry out aerobic fermentation, and round sealed thermal insulating, this stage does not ventilate in addition, Bacillus（It is aerobic）Using and disappear
The oxygen in round is consumed, is next stage to cause anaerobic environment.30 ~ 35 DEG C of fermentation temperature, 16 ~ 24h of fermentation time.
4 carry out anaerobic fermentation, lactic acid bacteria（Anaerobism）Being consumed in the round of oxygen in early stage carries out anaerobism
Ferment.35 ~ 37 DEG C of fermentation temperature, 18 ~ 20h of fermentation time.
The principle of institute's foundation of the present invention is：Add appropriate fermentable saccharide after queen bee tire is homogenized（Lactose）, using agricultural
Portion《Catalogue of feed additive varieties》The animal probio of middle announcement is fermented to pretreated queen bee tire homogenate, early stage
During aerobic fermentation, breaks down proteins into small-molecular peptides, are generated biologically active peptide by bacillus, while allergenic protein of degrading.
Under anaerobic, lactic acid bacteria can growth and breeding wherein so as to ferment, carbohydrate is hydrolyzed into into lactic acid etc., remove polypide fishy smell.
Meanwhile, inherently a kind of important beneficial bacteria of intestinal tract of lactic acid bacteria reaches animal intestinal position after intake, there is regulating intestinal canal flora
The effect of balance.
Compared with traditional simple animal probiotics preparation, beneficial effects of the present invention have it is following some：
（1）Queen bee fetoprotein by fermentation after, albumen therein is broken down into amino acid and small peptide, it is easier to inhaled by animal
Receive；
（2）Queen bee tire itself contained bioactivator such as chitin polysaccharide, vitamin, trace element, nucleic acid, flavones etc. are able to
Retain and become a part for prebiotics.
（3）First double section fermentation methods of aerobic anaerobism again, on the one hand allow Bacillus and lactic acid bacteria all sufficiently to be grown generation
Thank, on the other hand save extra anaerobic environment regulation and control, reduce preparation cost.
Specific embodiment
Technical scheme is further elaborated with reference to embodiment.
Embodiment 1
The step of present embodiments providing a kind of animal health queen bee tire probio oral liquid, the present embodiment is as follows：
（1）Pretreatment：Weigh 6kg queen bee tire homogenates, add 97g lactose, pasteurize 30 minutes at 55 DEG C after mixing.
（2）Pretreated queen bee tire homogenate is cooled to into less than 40 DEG C, the animal of the CFU/g of 6g 1.5 × 106 is added
The bacillus subtilis of Bifidobacterium powder, the CFU/g lactoenterococcus powder of 3 g 3.2 × 106 and 1 × 107CFU/g of 4g,
Stir.
（3）Postvaccinal queen bee tire homogenate is poured in closed round, in 34 DEG C of environment bottom fermentation 20h.So
After adjust the temperature to 37 DEG C, carry out anaerobic fermentation 18h, obtain the prebiotic oral liquid finished product of animal health queen bee tire.
Embodiment 2
Present embodiments provide application of the animal health of present invention announcement with queen bee tire probio oral liquid on piglet feed
1 materials and methods
1.1 test material
Queen bee tire probio oral liquid, main component queen bee fetoprotein zymotic fluid, containing animal bifidobacteria, lactoenterococcus and withered
Careless bacillus, total viable count 5.0 × 1011 CFU/L。
Experimental animal：The healthy tri-crossbreeding of body weight about 5.0kg 44, integrally sees that pig is in a good state of health.
1.2 experimental design
44 test pig parity are close, body weight is close, and the principle of male and female half and half is divided into 2 groups, per group of 3 repetitions, often repeatedly 6 ~
8.Control group fed basal diet（Original formula）；Test group（Queen bee tire probio oral liquid group）Feed in addition and drink 100mL
/ kg body weight queen bee tire probio oral liquids.Experimental design scheme is shown in Table 1.
1.3 test daily rations
Test daily ration is groove feed for pigling, feed composition and trophic level（Slightly）.
1.4 feeding and management
Test is carried out altogether 10 days.On-test is directly entered just tries the phase, does not do transition, feeds testing material.Conventinal breeding is managed,
Free choice feeding and drinking-water, immune disinfectant program is carried out according to pig farm conventional method.During test, observation each group swinery feeding daily
And health condition, accurate recording feed intake and diarrhoea situation.During off-test, weigh on an empty stomach by repetition.Calculating average daily gain,
Equal day feeding, feed-weight ratio and diarrhea rate.
1.5 data processing
Data processing and statistical analysis are carried out using SPSS statistical softwares, result of the test is represented with mean+SD, significantly
Property inspection adopt duncan's new multiple range method.
2 test results and analysis
Queen bee tire probio oral liquid is shown in Table 2 to the impact result of the test of Production Performance of Weaning Pigs and diarrhea rate
From table 2：Control group is compared, and queen bee tire probio oral liquid group feed-weight ratio and money meat ratio reduce respectively 15%（P<
0.01) with 9.11% (P<0.01), diarrhea rate significantly reduces 41.78% (P<0.01), average daily gain raises 19.2% (P<
0.05)。
3. conclusion
This result of the test shows, with queen bee tire probio oral liquid control group is substituted, and significantly improves the average daily gain of piglet,
Feed-weight ratio and money meat ratio are significantly reduced, and grice diarrhoea is substantially reduced.
Finally it should be noted that：Above specific description of embodiments of the present invention is not intended to limit the present invention, the technology of this area
Personnel carry out various modifications and improvements under the correlated condition of the present invention, should be included within the scope of the present invention.
Claims (5)
1. a kind of animal health queen bee tire probio oral liquid, is characterized in that, its preparation process is as follows：
1）, in queen bee tire homogenate add lactose, pasteurization is carried out after mixing；
2）, per milliliter of pretreated queen bee tire homogenate add animal probio based on total plate count 105~107 CFU；
3）, carry out aerobic fermentation, round sealed thermal insulating, this stage does not ventilate in addition, and Bacillus utilize and consume fermentation
Oxygen in container, is next stage to cause anaerobic environment.
2. 30 ~ 35 DEG C of fermentation temperature, 16 ~ 24h of fermentation time；
4）, carry out anaerobic fermentation, lactic acid bacteria has been consumed in the round of oxygen and has carried out anaerobic fermentation in early stage.
3. 35 ~ 37 DEG C of fermentation temperature, 18 ~ 20h of fermentation time.
4. animal health according to claim 1 queen bee tire probio oral liquid, is characterized in that described animal is prebiotic
Bacterium bag includes the bacillus licheniformis and bacillus subtilis of bacillus category, and enterococcus faecalis, the lactic acid intestines ball of lactic acid bacteria class
Bacterium, lactobacillus acidophilus, Lactobacillus plantarum, animal bifidobacteria, fermented bacterium is above-mentioned bacillus and lactic acid bacteria in use
Combination, i.e., at least include a kind of bacillus and a kind of lactic acid bacteria in fermented bacterium.
5. animal health according to claim 1 queen bee tire probio oral liquid, is characterized in that described pretreated
Queen bee tire homogenate, pretreatment mode is to add lactose in queen bee tire homogenate, and pasteurization, wherein lactose are carried out after mixing
Addition is in mass ratio：Queen bee tire homogenate:Lactose=100:1~100:2；Pasteurisation parameter is 55-60 DEG C, time 25-
30 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611205874.6A CN106615629A (en) | 2016-12-23 | 2016-12-23 | Bee larva probiotic oral liquid for animal healthcare |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611205874.6A CN106615629A (en) | 2016-12-23 | 2016-12-23 | Bee larva probiotic oral liquid for animal healthcare |
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| CN106615629A true CN106615629A (en) | 2017-05-10 |
Family
ID=58828146
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|---|---|---|---|
| CN201611205874.6A Pending CN106615629A (en) | 2016-12-23 | 2016-12-23 | Bee larva probiotic oral liquid for animal healthcare |
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|---|---|---|---|---|
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