CN107410363A - New bio control worm agent and preparation method thereof - Google Patents
New bio control worm agent and preparation method thereof Download PDFInfo
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- CN107410363A CN107410363A CN201710178064.4A CN201710178064A CN107410363A CN 107410363 A CN107410363 A CN 107410363A CN 201710178064 A CN201710178064 A CN 201710178064A CN 107410363 A CN107410363 A CN 107410363A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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Abstract
New bio control worm agent of the present invention and preparation method thereof is related to a kind of biocide for insect pest of the plant preventing and treating.Its purpose is to provide a kind of to control that worm effect is good, safe and non-toxic new bio control worm agent and preparation method thereof.New bio control worm agent of the present invention includes 0.05~0.15 part of propylene glycol alginate, 0.5~1.5 part of chitosan and 94~104 parts of prebiotic bacterium solutions according to mass fraction；The prebiotic bacterium solution includes according to mass fraction：89.5 parts of 6.5 parts of brown sugar, 6.5 parts of compound probiotic strain, 0.08 part of compound amino acid, 0.08 part of phosphoamide, 0.08 part of NaCl, 0.15 part of composite trace element, 0.08 part of Chinese herbal medicine and water.The product preparation method of the present invention, control worm effect is good and safe and non-toxic, is advantageous to environmental protection and sustainable development.The present invention is used for insect pest of the plant prevention and control field.
Description
Technical field
The present invention relates to a kind of biocide, more particularly to a kind of biocide for insect pest of the plant preventing and treating.
Background technology
It is at present to use agricultural chemicals for the most common way of insect pest, the most frequently used agricultural chemicals has Hostathion, phoxim, pyrrole worm
Quinoline, AVM, matrine etc..Studies in China development and application and formed commercially produced product mainly have bacterium insecticides,
Fungi insecticides, viral insecticides and antibiotic insecticide.
For the highly effective fruit of improvement of insect pest, but the resistance to the action of a drug of insect strengthens these medicaments year by year, and sprays medicine
Problem of environmental pollution, health problem caused by agent have caused everybody attention, and the low medicament even without toxicity of toxicity
But the effect of deinsectization is not reached.The biological insecticides sold on the market now are broadly divided into Dipel, insect viruses, plant
The major class of thing extract solution 3., can not for the insect pest occurred in agricultural environment but most of is selective pest control
Administered comprehensively.
The content of the invention
The technical problem to be solved in the present invention is to provide it is a kind of control good, the safe and non-toxic new bio control worm agent of worm effect and
Its preparation method, product safety of the invention is nontoxic, is advantageous to environmental protection and sustainable development.
The present invention relates to a kind of new bio control worm agent, the control worm agent includes 0.05~0.15 portion of algae according to mass fraction
Acid propylene glycol ester, 0.5~1.5 part of chitosan and 94~104 parts of prebiotic bacterium solutions；
The prebiotic bacterium solution includes according to mass fraction：6.5 parts of brown sugar, 6.5 parts of compound probiotic strain, compound amino acid
89.5 parts of 0.08 part, 0.08 part of phosphoamide, 0.08 part of NaCl, 0.15 part of composite trace element, 0.08 part of Chinese herbal medicine and water.
Preferably, it is described control worm agent according to mass fraction include 0.08~0.12 part of propylene glycol alginate, 0.8~1.2 part
Chitosan and 97~102 parts of prebiotic bacterium solutions.
Preferably, the control worm agent includes 0.1 part of propylene glycol alginate, 1 part of chitosan and 99 parts of benefits according to mass fraction
Raw bacterium solution.
Preferably, prebiotic bacterium solution includes acetobacter (Acetobacterium Balch et al.), saccharomyces cerevisiae
(Sac-charomycescerevisiae), lactobacillus acidophilus (Lactobacillus acidophilus), Lactobacillus casei
(L.casei), thermophilic salt actinomyces and Paenibacillus polymyxa (Paenibacillus polymyxa).
Preferably, the thermophilic salt actinomyces include rose pink mold cyst bacterium (Streptosporangium roseum), newly added
The polymorphic actinomyces in slope (Actinopolymorpha singaporensis) and salt tolerant nocardia (Nocardiopsis
halotolerans Al-Zarbanet al)。
The invention further relates to a kind of preparation method of new bio control worm agent, the described method comprises the following steps：
(1) 6.5 parts of brown sugar, 6.5 parts of compound probiotic strain, compound amino acid 0.08 are weighed respectively according to mass fraction
Part, 0.08 part of phosphoamide, 0.08 part of NaCl, 0.15 part of composite trace element, 0.08 part of Chinese herbal medicine and 89.5 parts of water；
(2) into brown sugar and compound amino acid plus water, while add waterside to stir, then add phosphoamide, composite trace element,
Chinese herbal medicine, NaCl, it is uniformly mixing to obtain mixed liquor 1；
(3) compound probiotic strain is added into mixed liquor 1, stirs 30min；
(4) ferment 40d, that is, prebiotic bacterium solution is made；
(5) 0.05~0.15 part of propylene glycol alginate, 0.5~1.5 part of chitosan and 94 are weighed respectively according to mass fraction
~104 parts of prebiotic bacterium solutions；
(6) prebiotic bacterium solution is placed in fermentation tank, starts to stir, be heated to 35~45 DEG C and keep, add alginic acid the third two
Alcohol ester, 1.5~2.5h is stood, is then stirred for 20~40min, 20~40min is again stirring for after 1.5~2.5h, stop adding
Heat, stand 20~28h；
(3) chitosan is added while stirring (to add in strain powdered chitosan in dispersed with spoon, make sure to keep in mind to allow
Chitosan is in lumps in strain), continue 30~40min of stirring, stand 4~6h, then stir 15~25min；
(4) new bio control worm agent of the invention is made after standing 10~14h.
Preferably, prebiotic bacterium solution includes acetobacter (Acetobacterium Balch et al.), saccharomyces cerevisiae
(Sac-charomycescerevisiae), lactobacillus acidophilus (Lactobacillus acidophilus), Lactobacillus casei
(L.casei), thermophilic salt actinomyces and Paenibacillus polymyxa (Paenibacillus polymyxa).
Preferably, the thermophilic salt actinomyces include rose pink mold cyst bacterium (Streptosporangium roseum), newly added
The polymorphic actinomyces in slope (Actinopolymorpha singaporensis) and salt tolerant nocardia (Nocardiopsis
halotolerans Al-Zarbanet al)。
Preferably, the mass fraction of propylene glycol alginate is 0.08~0.12 part, the quality of chitosan in the step (1)
Number is 0.8~1.2 part and the mass fraction of prebiotic bacterium solution is 97~102 parts.
Preferably, in the step (1) mass fraction of propylene glycol alginate be 0.1 part, the mass fraction of chitosan be 1
The mass fraction of part and prebiotic bacterium solution is 99 parts.
New bio control worm agent of the present invention and preparation method thereof difference from prior art is：
1 the present invention product be directed to all kinds of insect pests between difference, develop biology control worm agent series products, safety without
On the basis of poison, reach the effect of control insect pest.
The product utilization emplastic of 2 present invention, wraps insect, blocks insect esophagus and respiratory tract, insect is suffocated
It is and dead.The materials such as chitin, alginic acid contained by it can strengthen the system of defense of plant, improve crop resistance, and chitin
There is obvious action of contace poison to various aphids.
Contained probiotics can also promote plant growth in 3 products of the present invention, strengthen crop growing state.But also it can produce
Antibacterial material, suppress harmful microorganism breeding.The decomposition of soil surface organic matter can also be promoted in soil surface by spraying, and be suppressed
Soil surface characters grow.
The product of 4 present invention tackles the generation of insect pest by preventing, controlling two aspects, can not only solve farmland and currently occur
Pest problem, can also improve the resistance of crop itself, reduce insect pest incidence, ensure healthy growth of crops.
Embodiment
Made by the new bio control worm agent of following examples and checking test to the present invention and preparation method thereof further
Explanation.
Embodiment 1
The new bio control worm agent of the present embodiment is prepared according to the following steps：
(1) brown sugar 6.5kg, compound probiotic strain 6.5kg, compound amino acid 0.08kg, phosphoamide are weighed respectively
0.08kg, NaCl 0.08kg, composite trace element 0.15kg, Chinese herbal medicine 0.08kg and water 89.5kg；
(2) into brown sugar and compound amino acid plus water, while add waterside to stir, then add phosphoamide, composite trace element,
Chinese herbal medicine, NaCl, it is uniformly mixing to obtain mixed liquor 1；
(3) compound probiotic strain is added into mixed liquor 1, stirs 30min；
(4) ferment 40d, that is, prebiotic bacterium solution is made；
(5) 0.05kg propylene glycol alginates, 1.5kg chitosans and the prebiotic bacterium solutions of 94kg are weighed respectively；
(6) prebiotic bacterium solution is placed in fermentation tank, starts to stir, be heated to 35 DEG C and keep, add propylene glycol alginate,
1.5h is stood, is then stirred for 35min, 35min is again stirring for after 1.5h, stops heating, stands 26h；
(7) chitosan is added while stirring (to add in microbial inoculum powdered chitosan in dispersed with spoon, make sure to keep in mind to allow
Chitosan is in lumps in strain), continue to stir 30min, stand 6h, then stir 15min；
(8) new bio control worm agent of the invention is made after standing 13h.
The selection of strain is as shown in table 1 in prebiotic bacterium solution in the present embodiment.
Embodiment 2
The new bio control worm agent of the present embodiment is prepared according to the following steps：
(1) 0.15kg propylene glycol alginates, 0.5kg chitosans and the prebiotic bacterium solutions of 104kg are weighed respectively；
(2) prebiotic bacterium solution is placed in fermentation tank, starts to stir, be heated to 40 DEG C and keep, add propylene glycol alginate,
2h is stood, is then stirred for 30min, 25min is again stirring for after 2.5h, stops heating, stands 28h；
(3) chitosan is added while stirring (to add in microbial inoculum powdered chitosan in dispersed with spoon, make sure to keep in mind to allow
Chitosan is in lumps in strain), continue to stir 40min, stand 5h, then stir 25min；
(4) new bio control worm agent of the invention is made after standing 11h.
The selection of strain is as shown in table 1 in prebiotic bacterium solution in the present embodiment, preparation method and the phase of embodiment 1 of prebiotic bacterium solution
Together.
Embodiment 3
The new bio control worm agent of the present embodiment is prepared according to the following steps：
(1) 0.12kg propylene glycol alginates, 1kg chitosans and the prebiotic bacterium solutions of 99kg are weighed respectively；
(2) prebiotic bacterium solution is placed in fermentation tank, starts to stir, be heated to 38 DEG C and keep, add propylene glycol alginate,
1.8h is stood, is then stirred for 25min, 40min is again stirring for after 2h, stops heating, stands 22h；
(3) chitosan is added while stirring (to add in microbial inoculum powdered chitosan in dispersed with spoon, make sure to keep in mind to allow
Chitosan is in lumps in strain), continue to stir 35min, stand 5h, then stir 18min；
(4) new bio control worm agent of the invention is made after standing 14h.
The selection of strain is as shown in table 1 in prebiotic bacterium solution in the present embodiment, preparation method and the phase of embodiment 1 of prebiotic bacterium solution
Together.
Embodiment 4
The new bio control worm agent of the present embodiment is prepared according to the following steps：
(1) 0.08kg propylene glycol alginates, 1.2kg chitosans and the prebiotic bacterium solutions of 97kg are weighed respectively；
(2) prebiotic bacterium solution is placed in fermentation tank, starts to stir, be heated to 45 DEG C and keep, add propylene glycol alginate,
2.5h is stood, is then stirred for 40min, 30min is again stirring for after 1.5h, stops heating, stands 24h；
(3) chitosan is added while stirring (to add in microbial inoculum powdered chitosan in dispersed with spoon, make sure to keep in mind to allow
Chitosan is in lumps in strain), continue to stir 38min, stand 4h, then stir 20min；
(4) new bio control worm agent of the invention is made after standing 12h.
The selection of strain is as shown in table 1 in prebiotic bacterium solution in the present embodiment, preparation method and the phase of embodiment 1 of prebiotic bacterium solution
Together.
Embodiment 5
The new bio control worm agent of the present embodiment is prepared according to the following steps：
(1) 0.1kg propylene glycol alginates, 0.8kg chitosans and the prebiotic bacterium solutions of 102kg are weighed respectively；
(2) prebiotic bacterium solution is placed in fermentation tank, starts to stir, be heated to 42 DEG C and keep, add propylene glycol alginate,
2.2h is stood, is then stirred for 20min, 20min is again stirring for after 2.5h, stops heating, stands 20h；
(3) chitosan is added while stirring (to add in microbial inoculum powdered chitosan in dispersed with spoon, make sure to keep in mind to allow
Chitosan is in lumps in strain), continue to stir 32min, stand 6h, then stir 15min；
(4) new bio control worm agent of the invention is made after standing 10h.
The selection of strain is as shown in table 1 in prebiotic bacterium solution in the present embodiment, preparation method and the phase of embodiment 1 of prebiotic bacterium solution
Together.
In the embodiment 1-5 of table 1 in prebiotic bacterium solution strain selection
Note：Do not filled out as nothing in above table.
By the product being prepared in above-described embodiment 1-5 by sprayed after 500 times of volume dilution prevented and treated in Kiwi berry it is small green
Leafhopper, compared with spraying isoprocarb missible oil.Next day is observed, and sprays in the Kiwi berry fruit tree of isoprocarb missible oil and there are about 10-15%
Smaller green leaf hopper, the smaller green leaf hopper that there are about 10% or so on the 3rd is constant afterwards.Spray product next day fruit tree prepared by embodiment
In there are about 25% or so smaller green leaf hopper, second day there are about 10% or so, do not find then within 3rd.
After one month, the quantity for spraying smaller green leaf hopper in isoprocarb missible oil starts to increase, but sprays the region of this preparation then
Do not find smaller green leaf hopper.
The product being prepared in above-described embodiment 1-5 is prevented and treated into dish by being sprayed after 500 times of volume dilution in Chinese cabbage
Blue or green worm, it is very notable for the prevention effect of cabbage caterpillar compared with spraying mineral oil.Finally still there is 25- using the region of mineral oil
30% or so cabbage caterpillar is present, but the presence of cabbage caterpillar is hardly visible using the region of this product.
The product being prepared in above-described embodiment 1-5 is prevented and treated into white powder by being sprayed after 500 times of volume dilution in cucumber
Lice, made comparisons with spraying buprofezin missible oil (by 1000 times of volume dilution).Spraying the region of buprofezin missible oil can only see in a short time
To fragmentary trialeurodes vaporariorum, after 7-10, the quantity of trialeurodes vaporariorum gradually increases.Spray the region of this product white powder after 1-2 days
Lice is hardly visible, and does not still change after 7-10 days.
Empirical tests, the product for spraying the present invention inhale class formula insect, chewing pests and underground class pest for thorn
There is good prevention effect.
Although the foregoing describing the embodiment of the present invention, it will be appreciated by those of skill in the art that these
It is merely illustrative of, protection scope of the present invention is defined by the appended claims.Those skilled in the art is not carrying on the back
On the premise of principle and essence from the present invention, various changes or modifications can be made to these embodiments, but these are changed
Protection scope of the present invention is each fallen within modification.
Claims (10)
- A kind of 1. new bio control worm agent, it is characterised in that：The control worm agent includes 0.05~0.15 portion of algae according to mass fraction Acid propylene glycol ester, 0.5~1.5 part of chitosan and 94~104 parts of prebiotic bacterium solutions；The prebiotic bacterium solution includes according to mass fraction：6.5 parts of brown sugar, 6.5 parts of compound probiotic strain, compound amino acid 0.08 Part, 0.08 part of phosphoamide, 0.08 part of NaCl, 0.15 part of composite trace element, 0.08 part of Chinese herbal medicine and 89.5 parts of water.
- 2. new bio control worm agent according to claim 1, it is characterised in that：The control worm agent includes according to mass fraction 0.08~0.12 part of propylene glycol alginate, 0.8~1.2 part of chitosan and 97~102 parts of prebiotic bacterium solutions.
- 3. new bio control worm agent according to claim 2, it is characterised in that：The control worm agent includes according to mass fraction 0.1 part of propylene glycol alginate, 1 part of chitosan and 99 parts of prebiotic bacterium solutions.
- 4. the biological protection agent for disease control according to any one claim in claim 1-3, its feature It is：The prebiotic bacterium solution includes acetobacter, saccharomyces cerevisiae, lactobacillus acidophilus, Lactobacillus casei, thermophilic salt actinomyces and more Viscous series bacillus.
- 5. new bio control worm agent according to claim 4, it is characterised in that：The thermophilic salt actinomyces include rose chain spore Capsule bacterium, the polymorphic actinomyces of Singapore and salt tolerant nocardia.
- A kind of 6. preparation method of new bio control worm agent, it is characterised in that：It the described method comprises the following steps：(1) 6.5 parts of brown sugar, 6.5 parts of compound probiotic strain, 0.08 part of compound amino acid, carbon are weighed respectively according to mass fraction 89.5 parts of 0.08 part of acid amides, 0.08 part of NaCl, 0.15 part of composite trace element, 0.08 part of Chinese herbal medicine and water；(2) add water into brown sugar and compound amino acid, while adding waterside to stir, then add phosphoamide, composite trace element, medium-height grass Medicine, NaCl, it is uniformly mixing to obtain mixed liquor 1；(3) compound probiotic strain is added into mixed liquor 1, stirs 30min；(4) ferment 40d, that is, prebiotic bacterium solution is made；(5) weighed respectively according to mass fraction 0.05~0.15 part of propylene glycol alginate, 0.5~1.5 part of chitosan and 94~ 104 parts of prebiotic bacterium solutions；(6) prebiotic bacterium solution is placed in fermentation tank, starts to stir, be heated to 35~45 DEG C and keep, add propylene glycol alginate, 1.5~2.5h is stood, is then stirred for 20~40min, 20~40min is again stirring for after 1.5~2.5h, stops heating, it is quiet Put 20~28h；(7) chitosan is added while stirring, continues 30~40min of stirring, stands 4~6h, then stirs 15~25min；(8) new bio control worm agent of the invention is made after standing 10~14h.
- 7. the preparation method of new bio control worm agent according to claim 6, it is characterised in that：Wrapped in the prebiotic bacterium solution Include acetobacter, saccharomyces cerevisiae, lactobacillus acidophilus, Lactobacillus casei, thermophilic salt actinomyces and Paenibacillus polymyxa.
- 8. the preparation method of new bio control worm agent according to claim 7, it is characterised in that：The thermophilic salt unwrapping wire bacterium bag Include rose pink mold cyst bacterium, the polymorphic actinomyces of Singapore and salt tolerant nocardia.
- 9. the preparation method of new bio control worm agent according to claim 6, it is characterised in that：Algae in the step (1) The mass fraction of acid propylene glycol ester is 0.08~0.12 part, the mass fraction of chitosan is 0.8~1.2 part and prebiotic bacterium solution Mass fraction is 97~102 parts.
- 10. the preparation method of new bio control worm agent according to claim 9, it is characterised in that：Algae in the step (1) The mass fraction of acid propylene glycol ester is 0.1 part, the mass fraction of chitosan is 1 part and the mass fraction of prebiotic bacterium solution is 99 parts.
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| CN108902202A (en) * | 2018-05-07 | 2018-11-30 | 北京百丰天下生物科技有限公司 | It is exclusively used in the biological prevention and control agent and preparation method thereof of preventing and controlling subterranean pest |
| CN109258694A (en) * | 2018-09-13 | 2019-01-25 | 北京百丰天下生物科技有限公司 | It is exclusively used in the biology control worm agent and preparation method thereof of sucking pest prevention and control |
| CN109156482A (en) * | 2018-09-27 | 2019-01-08 | 宁夏普惠农业生物科技有限公司 | Plant growth promoter and preparation method thereof and application method |
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Application publication date: 20171201 |