CN109258694A - It is exclusively used in the biology control worm agent and preparation method thereof of sucking pest prevention and control - Google Patents
It is exclusively used in the biology control worm agent and preparation method thereof of sucking pest prevention and control Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N53/00—Biocides, pest repellants or attractants, or plant growth regulators containing cyclopropane carboxylic acids or derivatives thereof
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- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/08—Alkali metal chlorides; Alkaline earth metal chlorides
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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Abstract
The present invention relates to a kind of biology control worm agent for being exclusively used in sucking pest prevention and control, and the mass fraction of each component is as follows, and compound probiotic agent is 95.64-105 parts, biological adhesion agent is 0.05-0.2 parts, alkaloid is 1-2 parts.The preparation method of biology control worm agent includes the following steps, carbon dioxide is poured into heating system and is vented and is added honey and wheat bran, heats and keeps the temperature；Compound probiotic strain, sealing stirring are added after the mixture of aforementioned preparation is cooled down；The mixed liquor of aforementioned preparation is transferred in fermentor through centrifugal pump and carries out temperature-variable fermentation, obtains compound probiotic agent；Compound probiotic agent is placed in fermentor, heat and is kept the temperature, biological adhesion agent stirring is added；Heating system constant temperature is opened to 40 DEG C, stirs and be added alkaloid, obtains the biology control worm agent for being exclusively used in sucking pest prevention and control.Biology control worm agent of the invention and preparation method thereof, formulation ingredients are simple, control worm effect is good, quick, toxicity is low, can promote plant growth, improve the disease and insect resistance of crop.
Description
Technical field
The invention belongs to agricultural pests Control Technology fields, and in particular to a kind of biology for being exclusively used in sucking pest prevention and control
Control worm agent and preparation method thereof.
Background technique
Sucking pest refers to that using aphid, aleyrodid etc. be main mouthpart as the one of pierce-suck type or file suction, piercing and sucking plant juice
A little pests, individual is small, and in field, the aggrieved shape of early period of origination is often unobvious, is easily ignored by people.But sucking pest is numerous
Grow it is very fast, can in a short period of time it is exponential rise, quantity it is extremely more, often infest spray, leaf, bud, bud and
On fruit, water is drawn, robs its nutrition, causes branches and leaves and steamed twisted roll song or even whole strain withered or dead, while disease can be induced
Evil, not only seriously affects economic benefit, pest itself is the communication media of virosis sometimes.
Currently, in order to increase control efficiency, usually using the high toxicity pesticide of internal-suction type, but inevitably increase after use and make
The pesticide residue content of object juice or fruit, seriously affects agricultural product security, although having yellow plate, pure biological agent etc., due to
Usage mode, the limitation in region etc., using effect is difficult to achieve the effect that desired.
Alkaloid is a kind of important natural organic compounds containing nitrogen, and about 20%~30% higher plant and part are low
Contain alkaloid in plant.Alkaloid can act on insect by various ways such as poisoning, deterrent, food refusal, inhibition growth and development.
Alkaloid with insecticidal activity is easy degradation in the environment, and the mode of action is unique, to beneficial organism safety, is not easy to pest
It develops drug resistance.But there are following major defects for current alkaloid insecticide: 1. most natural products compound structures are complicated, no
It is easily-synthesized or synthesis cost is too high；2. formulation ingredients are complicated, it is not easy to standardize；3. most plants insecticide plays drug effect
Slowly, some friends peasant is caused to think that used insecticide does not have effect etc..
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of low cost, formulation ingredients are simple, control worm effect is good, take effect
Fastly, hypotoxicity, the biology control worm agent that is exclusively used in sucking pest prevention and control non-hazardous to biology outside target and Environmental security, this hair
Bright product is also divided using complex microorganism for the facilitation of plant and to toxicant other than the prevention and control to pest
Solution effect, promotes the growth of crop, improves the disease and insect resistance of crop, plays the purpose of multiple-effect unification.
It is special the present invention relates to a kind of biology control worm agent for being exclusively used in sucking pest prevention and control, including compound probiotic agent
Sign is: further including biological adhesion agent and alkaloid, the mass fraction of each component is as follows in the biology control worm agent, compound prebiotic
Microbial inoculum is 95.64-105 parts, biological adhesion agent is 0.05-0.2 parts, alkaloid is 1-2 parts.
The compound probiotic agent includes 4-6 parts of honey, 4-6 parts of compound probiotic strains, 0.4-0.6 according to mass fraction
Part wheat bran, 0.08-0.12 parts of carbamides, 0.08-0.12 parts of sodium chloride, 0.008-0.012 parts of Chinese herbal medicines, 0.08-0.15 portions of paddy
Propylhomoserin sodium and 87-92 parts of water.
Preferably, the compound probiotic agent according to mass fraction include 4.5-5.5 parts of honey, 4.5-5.5 parts it is compound
Probiotics strain, 0.45-0.55 part wheat bran, 0.09-0.11 parts of carbamides, 0.09-0.11 parts of sodium chloride, 0.009-0.011 parts
Chinese herbal medicine, 0.09-0.13 part sodium glutamate and 89-91 parts of water.
In any of the above-described scheme preferably, the compound probiotic agent according to mass fraction include 5.5 parts of honey,
5.5 parts of compound probiotic strains, 0.5 part of wheat bran, 0.09 part of carbamide, 0.11 part of sodium chloride, 0.009 part of Chinese herbal medicine, 0.12 part
Sodium glutamate and 89 parts of water.
In any of the above-described scheme preferably, the Chinese herbal medicine includes one of Radix Isatidis, fructus lycii, ginseng or several
Kind.
In any of the above-described scheme preferably, the compound probiotic strain includes acetobacter, saccharomyces cerevisiae, acidophilus
One or more of lactobacillus, Lactobacillus casei, methamidophos, thermophilic salt actinomyces and Paenibacillus polymyxa.
In any of the above-described scheme preferably, the compound probiotic strain include 2% viable count >=30,000,000,000/gram
Acetobacter, 27% viable count >=20,000,000,000/gram saccharomyces cerevisiae, 6% viable count >=60,000,000,000/gram lactobacillus acidophilus, 51%
Viable count >=30,000,000,000/gram Lactobacillus casei, 12% viable count >=20,000,000,000/gram methamidophos, 1% viable count >=80
Hundred million/gram thermophilic salt actinomyces and 1% viable count >=10,000,000,000/gram Paenibacillus polymyxa.
In any of the above-described scheme preferably, the alkaloid includes one of pyrethrin, matrine, margosine
Or it is several.
In any of the above-described scheme preferably, in the alkaloid mass fraction of pyrethrin be 0.3-0.7 part, hardship
The mass fraction for joining alkali is 0.3-0.7 parts, the mass fraction of margosine is 0.4-0.6 parts.
In any of the above-described scheme preferably, the mass fraction of pyrethrin is 0.3 part, matrine in the alkaloid
Mass fraction be 0.3 part, the mass fraction of margosine is 0.4 part.
Prevention and treatment of the pyrethrin as alkaloid for sanitary insect pest is with a long history, is mostly with mosquito control, flea, louse
It is main；Matrine is a kind of wide spectrum minuent insecticide, has to pest and tags and stomach poison function, pest once contact medicament, will
Make it finally because stomata is blocked due to death by vivo protein；Margosine is that a kind of have stomach toxicity, tag and Antifeedant Effects, pest take
After food and contact chinaberry element, can the conduction of block nerves maincenter, destroy midgut tissue and various Chlorogenic Acids and respiratory metabolism, shadow
It rings and digests and assimilates, lose to food taste function, cause pest growth and development abnormal and dead with food refusal, it can also be in husking
It forms lopsided polypide and goes into a coma lethal.Usual margosine has very high bioactivity to a variety of lepidoptera pests, but to piercing and sucking
Formula mouthpart insect is without preventive effect.Single present invention cooperated using three kinds of alkaloids, has been made a kind of and has been exclusively used in sucking pest prevention and control
Biology control worm agent, worm effect is good for control, quick, hypotoxicity, non-hazardous to biology and Environmental security outside target.
In any of the above-described scheme preferably, the biology adhesion agent includes guar gum, sodium alginate, in chitosan
It is one or more of.
Any of the above-described biological preparation method for controlling worm agent for being exclusively used in sucking pest prevention and control is produced the present invention relates to a kind of,
According to sequencing the following steps are included:
(1) weighed respectively according to mass fraction honey, compound probiotic strain, wheat bran, carbamide, sodium chloride, Chinese herbal medicine,
Sodium glutamate and water；
(2) carbon dioxide is poured into heating system is vented and is added honey and wheat bran, it is close when temperature rises to 85 DEG C
Seal 30min；Then it ventilates plus water persistently stirs, be then separately added into the originals such as carbamide, sodium chloride, Chinese herbal medicine, sodium glutamate
Material；
(3) compound probiotic strain, sealing stirring are added after the mixture of above-mentioned steps (2) step being cooled to 38 DEG C
30min；
(4) mixed liquor in above-mentioned steps (3) is transferred to progress temperature-variable fermentation 20-30d in fermentor through centrifugal pump, i.e.,
Compound probiotic agent is made；
(5) pyrethrin, matrine, margosine are weighed respectively according to mass fraction, alkaloid is made；
(6) weighed respectively according to mass fraction compound probiotic agent made from above-mentioned steps (1)-(4) step, biology it is sticky
Agent, alkaloid；
(7) compound probiotic agent is placed in fermentor, starts to stir, be heated to 35~45 DEG C and kept, biology is added
Adhesion agent persistently stirs 60min, stirs 30min after standing 10min, stops heating and stirring；
(8) it places and is again turned on heating system constant temperature afterwards for 24 hours to 40 DEG C, be slowly added to the biology got ready after stirring 20min
Alkali obtains the biology control worm agent for being exclusively used in sucking pest prevention and control of the invention after at least stirring 30min after addition；
(9) finished product stores.
It preferably, include acetobacter (Acetobacterium Balch et al.), wine brewing ferment in compound probiotic kind
Mother (Sac-charomyces cerevisiae), lactobacillus acidophilus (Lactobacillus acidophilus), cheese cream bar
Bacterium (L.casei), thermophilic salt actinomyces and Paenibacillus polymyxa (Paenibacilluspolymyxa).
Preferably, the thermophilic salt actinomyces include rose pink mold cyst bacterium (Streptosporangium roseum), newly add
The polymorphic actinomyces in slope (Actinopolymorpha singaporensis) and salt tolerant nocardia (Nocardiopsis
halotoleransAl-Zarban etal)。
The preparation method difference from prior art of the biology control worm agent for being exclusively used in sucking pest prevention and control of the invention
It is:
1 product of the invention reaches on the basis of safe and non-toxic specifically for the biology control worm agent that sucking pest is researched and developed
To the effect of efficient control insect pest；
2 product utilization biology adhesion agents of the invention wrap pest, block pest esophagus and respiratory tract, stop up pest
Breath and it is dead；
3 alkaloids of the invention include pyrethrin, matrine, margosine, surmount the prevention and treatment that the prior art reaches best
The effect of pest；
Compound probiotic agent contained in 4 product of the present invention can also promote plant growth, enhance crop growing state.But also
Antibacterial material can be generated, harmful microorganism breeding is inhibited.Point of soil surface organic matter can also be promoted in soil surface by spraying
Solution inhibits soil surface characters to breed；
5 products of the invention cope with the generation of insect pest by preventing, controlling two aspects, not only can solve farmland and currently occur
Pest problem, can also improve the resistance of crop itself, reduce insect pest incidence, guarantee healthy growth of crops.
All multi-parameters involved in technical solution of the present invention, need to comprehensively consider the synergistic effect between parameters,
Beneficial effects of the present invention and marked improvement could be obtained.And the value range of parameters is all through excessive in technical solution
Amount test just obtains, and for the intercombination of each parameter and parameters, inventor has recorded a large number of experiments number
According to, as space is limited, underground specific test data herein.
Specific embodiment
The biology control worm agent for being exclusively used in sucking pest prevention and control to of the invention one by following embodiment and verification test
And preparation method thereof be further described.
Embodiment 1
A kind of biology control worm agent being exclusively used in sucking pest prevention and control of the present embodiment is prepared according to the following steps:
(1) honey 4kg, compound probiotic strain 4kg, wheat bran 0.4kg, carbamide 0.08kg, sodium chloride are weighed respectively
0.08kg, Chinese herbal medicine 0.008kg, sodium glutamate 0.08kg and water 87kg；
(2) carbon dioxide is poured into heating system is vented and is added honey and wheat bran, it is close when temperature rises to 85 DEG C
Seal 30min；Then it ventilates plus water persistently stirs, be then separately added into the originals such as carbamide, sodium chloride, Chinese herbal medicine, sodium glutamate
Material；
(3) compound probiotic strain, sealing stirring 30min are added after the mixture of above-mentioned (2) step being cooled to 38 DEG C；
(4) mixed liquor in above-mentioned (3) is transferred to progress temperature-variable fermentation 20d in fermentor through centrifugal pump, obtained multiple
Close probiotics；
(5) 0.6 part of pyrethrin, 0.3 part of matrine, 0.6 part of margosine obtained biology are weighed respectively according to mass fraction
Alkali；
(6) compound probiotic agent 96kg, guar gum 0.1kg, alkaloid made from above-mentioned (1)-(4) step are weighed respectively
1.5kg；
(7) compound probiotic agent is placed in fermentor, the content of each bacterium and addition number in compound probiotic strain
As shown in table 1, start to stir, be heated to 35 DEG C and keep, biological adhesion agent is added and persistently stirs 60min, is stirred after standing 10min
30min is mixed, heating and stirring are stopped；
(8) it places and is again turned on heating system constant temperature afterwards for 24 hours to 40 DEG C, be slowly added to the biology got ready after stirring 20min
Alkali obtains the biology control worm agent for being exclusively used in sucking pest prevention and control of the invention after at least stirring 30min after addition；
(9) finished product stores.
Chinese herbal medicine is Radix Isatidis, fructus lycii and the ginseng that mass ratio is 1:1:1 in the present embodiment；Compound probiotic strain is vinegar
Acidfast bacilli (Acetobacterium Balch), saccharomyces cerevisiae (Sac-charomyces cerevisiae), lactobacillus acidophilus
(Lactobacillus acidophilus), methamidophos (Zygosaccharomyces rouxii), thermophilic salt actinomyces,
Lactobacillus casei (L.casei) and Paenibacillus polymyxa (Paenibacilluspolymyxa)；Wherein, thermophilic salt unwrapping wire bacterium bag
Include rose pink mold cyst bacterium (Streptosporangium roseum), the polymorphic actinomyces (Actinopolymorpha of Singapore
Singaporensis), salt tolerant nocardia (Nocardiopsis halotoleransAl-Zarban etal).
Embodiment 2
A kind of biology control worm agent being exclusively used in sucking pest prevention and control of the present embodiment is prepared according to the following steps:
(1) honey 6kg, compound probiotic strain 6kg, wheat bran 0.6kg, carbamide 0.12kg, sodium chloride are weighed respectively
0.12kg, Chinese herbal medicine 0.012kg, sodium glutamate 0.15kg and water 92kg；
(2) carbon dioxide is poured into heating system is vented and is added honey and wheat bran, it is close when temperature rises to 85 DEG C
Seal 30min；Then it ventilates plus water persistently stirs, be then separately added into the originals such as carbamide, sodium chloride, Chinese herbal medicine, sodium glutamate
Material；
(3) compound probiotic strain, sealing stirring 30min are added after the mixture of above-mentioned (2) step being cooled to 38 DEG C；
(4) mixed liquor in above-mentioned (3) is transferred to progress temperature-variable fermentation 25d in fermentor through centrifugal pump, obtained multiple
Close probiotics；
(5) 0.7 part of pyrethrin, 0.4 part of matrine, 0.6 part of margosine obtained biology are weighed respectively according to mass fraction
Alkali；
(6) compound probiotic agent 98.2kg, guar gum 0.05, alginic acid made from above-mentioned (1)-(4) step are weighed respectively
Sodium 0.05kg, alkaloid 1.7kg；
(7) compound probiotic agent is placed in fermentor, the content of each bacterium and addition number in compound probiotic strain
As shown in table 1, start to stir, be heated to 40 DEG C and keep, biological adhesion agent is added and persistently stirs 60min, is stirred after standing 10min
30min is mixed, heating and stirring are stopped；
(8) it places and is again turned on heating system constant temperature afterwards for 24 hours to 40 DEG C, be slowly added to the biology got ready after stirring 20min
Alkali obtains the biology control worm agent for being exclusively used in sucking pest prevention and control of the invention after at least stirring 30min after addition；
(9) finished product stores.
Chinese herbal medicine is Radix Isatidis, fructus lycii and the ginseng that mass ratio is 1:2:1 in the present embodiment；Compound probiotic strain is vinegar
Acidfast bacilli (Acetobacterium Balch), saccharomyces cerevisiae (Sac-charomyces cerevisiae), lactobacillus acidophilus
(Lactobacillus acidophilus), methamidophos (Zygosaccharomyces rouxii), thermophilic salt actinomyces,
Lactobacillus casei (L.casei) and Paenibacillus polymyxa (Paenibacilluspolymyxa)；Wherein, thermophilic salt unwrapping wire bacterium bag
Include rose pink mold cyst bacterium (Streptosporangium roseum), the polymorphic actinomyces (Actinopolymorpha of Singapore
Singaporensis), salt tolerant nocardia (Nocardiopsis halotoleransAl-Zarban etal).
Embodiment 3
A kind of biology control worm agent being exclusively used in sucking pest prevention and control of the present embodiment is prepared according to the following steps:
(1) honey 4.5kg, compound probiotic strain 4.5kg, wheat bran 0.45kg, carbamide 0.09kg, chlorination are weighed respectively
Sodium 0.09kg, Chinese herbal medicine 0.009kg, sodium glutamate 0.09kg and water 89kg；
(2) carbon dioxide is poured into heating system is vented and is added honey and wheat bran, it is close when temperature rises to 85 DEG C
Seal 30min；Then it ventilates plus water persistently stirs, be then separately added into the originals such as carbamide, sodium chloride, Chinese herbal medicine, sodium glutamate
Material；
(3) compound probiotic strain, sealing stirring 30min are added after the mixture of above-mentioned (2) step being cooled to 38 DEG C；
(4) mixed liquor in above-mentioned (3) is transferred to progress temperature-variable fermentation 30d in fermentor through centrifugal pump, obtained multiple
Close probiotics；
(5) 0.7 part of pyrethrin, 0.7 part of matrine, 0.6 part of margosine obtained biology are weighed respectively according to mass fraction
Alkali；
(6) compound probiotic agent 100kg, guar gum 0.1kg, alginic acid made from above-mentioned (1)-(4) step are weighed respectively
Sodium 0.05kg, chitosan 0.05kg, alkaloid 2kg；
(7) compound probiotic agent is placed in fermentor, the content of each bacterium and addition number in compound probiotic strain
As shown in table 1, start to stir, be heated to 45 DEG C and keep, biological adhesion agent is added and persistently stirs 60min, is stirred after standing 10min
30min is mixed, heating and stirring are stopped；
(8) it places and is again turned on heating system constant temperature afterwards for 24 hours to 40 DEG C, be slowly added to the biology got ready after stirring 20min
Alkali obtains the biology control worm agent for being exclusively used in sucking pest prevention and control of the invention after at least stirring 30min after addition；
(9) finished product stores.
Chinese herbal medicine is Radix Isatidis, fructus lycii and the ginseng that mass ratio is 1:1:2 in the present embodiment；Compound probiotic strain is vinegar
Acidfast bacilli (Acetobacterium Balch), saccharomyces cerevisiae (Sac-charomyces cerevisiae), lactobacillus acidophilus
(Lactobacillus acidophilus), methamidophos (Zygosaccharomyces rouxii), thermophilic salt actinomyces,
Lactobacillus casei (L.casei) and Paenibacillus polymyxa (Paenibacilluspolymyxa)；Wherein, thermophilic salt unwrapping wire bacterium bag
Include rose pink mold cyst bacterium (Streptosporangium roseum), the polymorphic actinomyces (Actinopolymorpha of Singapore
Singaporensis), salt tolerant nocardia (Nocardiopsis halotoleransAl-Zarban etal).
Embodiment 4
A kind of biology control worm agent being exclusively used in sucking pest prevention and control of the present embodiment is prepared according to the following steps:
(1) honey 5.5kg, compound probiotic strain 5.5kg, wheat bran 0.5kg, carbamide 0.09kg, chlorination are weighed respectively
Sodium 0.11kg, Chinese herbal medicine 0.009kg, sodium glutamate 0.12kg and water 89kg；
(2) carbon dioxide is poured into heating system is vented and is added honey and wheat bran, it is close when temperature rises to 85 DEG C
Seal 30min；Then it ventilates plus water persistently stirs, be then separately added into the originals such as carbamide, sodium chloride, Chinese herbal medicine, sodium glutamate
Material；
(3) compound probiotic strain, sealing stirring 30min are added after the mixture of above-mentioned (2) step being cooled to 38 DEG C；
(4) mixed liquor in above-mentioned (3) is transferred to progress temperature-variable fermentation 30d in fermentor through centrifugal pump, obtained multiple
Close probiotics；
(5) 0.3 part of pyrethrin, 0.3 part of matrine, 0.4 part of margosine obtained biology are weighed respectively according to mass fraction
Alkali；
(6) compound probiotic agent 95kg, guar gum 0.05kg, alginic acid made from above-mentioned (1)-(4) step are weighed respectively
Sodium 0.03kg, chitosan 0.02kg, alkaloid 1kg；
(7) compound probiotic agent is placed in fermentor, the content of each bacterium and addition number in compound probiotic strain
As shown in table 1, start to stir, be heated to 45 DEG C and keep, biological adhesion agent is added and persistently stirs 60min, is stirred after standing 10min
30min is mixed, heating and stirring are stopped；
(8) it places and is again turned on heating system constant temperature afterwards for 24 hours to 40 DEG C, be slowly added to the biology got ready after stirring 20min
Alkali obtains the biology control worm agent for being exclusively used in sucking pest prevention and control of the invention after at least stirring 30min after addition；
(9) finished product stores.
Chinese herbal medicine is Radix Isatidis, fructus lycii and the ginseng that mass ratio is 2:1:1 in the present embodiment；Compound probiotic strain is vinegar
Acidfast bacilli (Acetobacterium Balch), saccharomyces cerevisiae (Sac-charomyces cerevisiae), lactobacillus acidophilus
(Lactobacillus acidophilus), methamidophos (Zygosaccharomyces rouxii), thermophilic salt actinomyces,
Lactobacillus casei (L.casei) and Paenibacillus polymyxa (Paenibacilluspolymyxa)；Wherein, thermophilic salt unwrapping wire bacterium bag
Include rose pink mold cyst bacterium (Streptosporangium roseum), the polymorphic actinomyces (Actinopolymorpha of Singapore
Singaporensis), salt tolerant nocardia (Nocardiopsis halotoleransAl-Zarban etal).
The content of each bacterium in 1 compound probiotic strain of table
Verification test
Confirmatory experiment 1
Place: positioned at the Si Kailande ecological, environmental protective garden in Beijing Haidian
In garden carry out celery plantation when, due to climatic factor, aphid is caused largely to be broken out, young leaves is rolled up, growth by
Resistance after growing area is divided into two, sprays said preparation in East.After 3 days, hence it is evident that see that young leaves starts to sprout, the harm of aphid
Pass by, after two weeks, celery growing way is vigorous, does not obtain aphid again later.It reviews and does not spray region, until beginning to whole not put
The harm of de- aphid, the death rate is more than 40%, and harvest is below 5%.
Confirmatory experiment 2
Place: Huailai, Hebei farm
All kinds of vanillas of grower in a region on farm, early growth is vigorous, but with suffering from trialeurodes vaporariorum calamity
Evil, the growth of vanilla is hindered, even if spray other medicaments, sprays the same day effectively, but second day trialeurodes vaporariorum is just returned again
?.After having sprayed said preparation, due to the presence of adhesion agent, aleyrodid movement is obstructed, and after sprinkling is primary, just solves trialeurodes vaporariorum
The problem of.
Although specific embodiments of the present invention have been described above, it will be appreciated by those of skill in the art that these
It is merely illustrative of, protection scope of the present invention is defined by the appended claims.Those skilled in the art is not carrying on the back
Under the premise of from the principle and substance of the present invention, various changes or modifications can be made to these embodiments, but these are changed
Protection scope of the present invention is each fallen with modification.
Claims (10)
1. a kind of biology control worm agent for being exclusively used in sucking pest prevention and control, including compound probiotic agent, it is characterised in that: further include
Biological adhesion agent and alkaloid, the mass fraction that the biology controls each component in worm agent is as follows, and compound probiotic agent is 95.64-
105 parts, biological adhesion agent be 0.05-0.2 parts, alkaloid is 1-2 parts.
2. the biology control worm agent according to claim 1 for being exclusively used in sucking pest prevention and control, it is characterised in that: described compound
Probiotics include 4-6 parts of honey, 4-6 parts of compound probiotic strains, 0.4-0.6 parts of wheat brans, 0.08-0.12 according to mass fraction
Part carbamide, 0.08-0.12 parts of sodium chloride, 0.008-0.012 parts of Chinese herbal medicines, 0.08-0.15 parts of sodium glutamates and 87-92 parts
Water.
3. the biology control worm agent according to claim 2 for being exclusively used in sucking pest prevention and control, it is characterised in that: described compound
Probiotics include 4.5-5.5 parts of honey, 4.5-5.5 parts of compound probiotic strains, 0.45-0.55 parts of brans according to mass fraction
Skin, 0.09-0.11 part carbamide, 0.09-0.11 parts of sodium chloride, 0.009-0.011 parts of Chinese herbal medicines, 0.09-0.13 parts of glutamic acid
Sodium and 89-91 parts of water.
4. the biology control worm agent according to claim 3 for being exclusively used in sucking pest prevention and control, it is characterised in that: the medium-height grass
Medicine includes one or more of Radix Isatidis, fructus lycii, ginseng.
5. being exclusively used in the biology control worm agent of sucking pest prevention and control described in any one of -4 according to claim 1, feature exists
In: the compound probiotic strain include acetobacter, saccharomyces cerevisiae, lactobacillus acidophilus, Lactobacillus casei, methamidophos,
One or more of thermophilic salt actinomyces and Paenibacillus polymyxa.
6. the biology control worm agent according to claim 5 for being exclusively used in sucking pest prevention and control, it is characterised in that: described compound
Probiotics strain include 2% viable count >=30,000,000,000/gram acetobacter, 27% viable count >=20,000,000,000/gram saccharomyces cerevisiae,
6% viable count >=60,000,000,000/gram lactobacillus acidophilus, 51% viable count >=30,000,000,000/gram Lactobacillus casei, 12% viable count >=
20000000000/gram methamidophos, 1% viable count >=8,000,000,000/gram thermophilic salt actinomyces and 1% viable count >=10,000,000,000/gram it is more
Viscous series bacillus.
7. the biology control worm agent according to claim 1 for being exclusively used in sucking pest prevention and control, it is characterised in that: the biology
Alkali includes one or more of pyrethrin, matrine, margosine.
8. the biology control worm agent according to claim 7 for being exclusively used in sucking pest prevention and control, it is characterised in that: the deinsectization
The mass fraction of chrysanthemum element is 0.3-0.7 parts, and the mass fraction of the matrine is 0.3-0.7 parts, the mass parts of the margosine
Number is 0.4-0.6 parts.
9. the biology control worm agent according to claim 1 for being exclusively used in sucking pest prevention and control, it is characterised in that: the biology
Adhesion agent includes one or more of guar gum, sodium alginate, chitosan.
10. the preparation side of the biology control worm agent according to claim 1 to 9 for being exclusively used in sucking pest prevention and control
Method, according to sequencing the following steps are included:
(1) honey, compound probiotic strain, wheat bran, carbamide, sodium chloride, Chinese herbal medicine, paddy ammonia are weighed respectively according to mass fraction
Sour sodium and water；
(2) carbon dioxide is poured into heating system is vented and is added honey and wheat bran, when temperature rises to 85 DEG C, sealing
30min；Then it ventilates plus water persistently stirs, be then separately added into the raw materials such as carbamide, sodium chloride, Chinese herbal medicine, sodium glutamate；
(3) compound probiotic strain, sealing stirring 30min are added after the mixture of above-mentioned steps (2) being cooled to 38 DEG C；
(4) mixed liquor in above-mentioned steps (3) is transferred to progress temperature-variable fermentation 20-30d in fermentor through centrifugal pump, obtained
Compound probiotic agent；
(5) pyrethrin, matrine, margosine are weighed respectively according to mass fraction, alkaloid is made；
(6) compound probiotic agent made from above-mentioned steps (1)-(4), biological adhesion agent, biology are weighed respectively according to mass fraction
Alkali；
(7) compound probiotic agent is placed in fermentor, starts to stir, be heated to 35~45 DEG C and kept, it is sticky that biology is added
60min is persistently stirred in agent, stirs 30min after standing 10min, stops heating and stirring；
(8) it places and is again turned on heating system constant temperature afterwards for 24 hours to 40 DEG C, be slowly added to the alkaloid got ready after stirring 20min, add
The biology control worm agent for being exclusively used in sucking pest prevention and control is obtained after at least stirring 30min after adding；
(9) finished product stores.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811069108.0A CN109258694A (en) | 2018-09-13 | 2018-09-13 | It is exclusively used in the biology control worm agent and preparation method thereof of sucking pest prevention and control |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811069108.0A CN109258694A (en) | 2018-09-13 | 2018-09-13 | It is exclusively used in the biology control worm agent and preparation method thereof of sucking pest prevention and control |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104273179A (en) * | 2013-07-11 | 2015-01-14 | 青岛罗素生物技术有限公司 | Preparation method of oxymatrine aqueous solution |
| CN107410363A (en) * | 2017-03-23 | 2017-12-01 | 北京百丰天下生物科技有限公司 | New bio control worm agent and preparation method thereof |
| CN107568249A (en) * | 2017-07-19 | 2018-01-12 | 北京百丰天下生物科技有限公司 | Bioprotective applied to prevention and cure of viruses and preparation method thereof |
-
2018
- 2018-09-13 CN CN201811069108.0A patent/CN109258694A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104273179A (en) * | 2013-07-11 | 2015-01-14 | 青岛罗素生物技术有限公司 | Preparation method of oxymatrine aqueous solution |
| CN107410363A (en) * | 2017-03-23 | 2017-12-01 | 北京百丰天下生物科技有限公司 | New bio control worm agent and preparation method thereof |
| CN107568249A (en) * | 2017-07-19 | 2018-01-12 | 北京百丰天下生物科技有限公司 | Bioprotective applied to prevention and cure of viruses and preparation method thereof |
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Application publication date: 20190125 |