CN108094708A - A kind of feed addictive of the slag containing Cortex Eucommiae and preparation method thereof - Google Patents
A kind of feed addictive of the slag containing Cortex Eucommiae and preparation method thereof Download PDFInfo
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
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- A23K50/00—Feeding-stuffs specially adapted for particular animals
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- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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Abstract
Feed addictive the present invention relates to a kind of slag containing Cortex Eucommiae and preparation method thereof, including following raw material：Cortex Eucommiae slag, herba houttuyniae powder and Canton love-pea vine powder, its preparation method are：(1) Cortex Eucommiae slag, herba houttuyniae powder and Canton love-pea vine powder are mixed, obtains mixture；(2) step (1) made mixture is added in together with zymophyte, water, glucose in solid-state fermenter and fermented；(3) fermentate obtained by step (2) is manufactured into the particle that grain size is 0.5~1cm, it is dry, both feed addictive of the present invention.A kind of feed addictive of slag containing Cortex Eucommiae of the present invention, take full advantage of active ingredient contained in Cortex Eucommiae slag, pass through the method for solid state fermentation, make in feed addictive rich in enzymes such as amylase, acid protease, cellulase, pectases, complex enzyme formulation needed for alternative feed, and significantly improve the protein content in feed addictive.By feed addictive of the present invention for layer breeding, laying rate of laying hen and egg size can be significantly improved.
Description
Technical field
The present invention relates to feed additive fields, and in particular to a kind of feed addictive of slag containing Cortex Eucommiae and its preparation side
Method.
Background technology
With the fast development of aquaculture, feedstuff industry also accelerate its develop step, and feed addictive be feed feed
Foster necessary component, the use of additive are directly related to the quality of meat, egg, milk.The existing feed addictive overwhelming majority
Containing antibiotic, and after antibiotic takes in human body by animal for a long time, the immunity of animal organism can be made gradually to weaken, so as to lead
The chronic disease of actuating object increases, and the edible livestock product fed containing antibiotic feed will also result in health hazard；And it abuses
Antibiotic can cause flora imbalance and autogenous infection in animal body, increase aquaculture cost.As people are to nutrition, food
The attention of safety and environmental quality, more and more consumingly requirement is forbidden using antibiotic and someization in food, animal feed
Learn synthetic drug.European Union has completely forbidden makees feed addictive using antibiotic, substitutes chemical synthetic drug with Chinese herbal medicine, resists
Raw element class additive has extensive prospect.During although the medicated feed additive of some current enterprises of China is used for
Herbal medicine, but antibiotic and chemical synthetic drug still account for the major part of feed addictive.
Cortex Eucommiae is China's parts of generic medicinal plants, containing chlorogenic acid, flavones, polysaccharide, crude protein, crude fibre etc. it is a variety of it is medicinal into
Point and nutritional ingredient, be both included in the Ministry of Public Health can be used for health food item categories, be also put into Ministry of Agriculture's new edition《Feed is former
Expect catalogue (2012)》.China's eucommia resource enriches, also relatively more as the plant extract enterprise of raw material using Cortex Eucommiae and folium cortex eucommiae,
The substantial amounts of Cortex Eucommiae dregs of a decoction have been discarded in annual processing, not only take away a large amount of leaching liquors, and are consumed waste residue transport and concentrated landfill
A large amount of expenses of processing.To prevent the dregs of a decoction from flowing into illegal channels, requirement Chinese medicine manufacturing enterprise of State Food and Drug Administration
It should strengthen the management to the dregs of a decoction after extraction, promote reasonable employment, establish dregs of a decoction disposal system, specify dregs of a decoction quantity, flow direction, use
Way etc., foundation can trace even reading registration archives, the dregs of a decoction that can isolate complete medicinal material or medicine materical crude slice form should be taken and unmake
Measure prevents from flowing into medicinal material or medicine materical crude slice intermediate links.It would therefore be highly desirable to the dregs of a decoction discarded to Cortex Eucommiae extraction are strengthened management and utilized.
" a kind of folium cortex eucommiae fermented feed and its production method " disclosed in Chinese patent application
(CN201510668036.1), it is Cortex Eucommiae wet dreg after folium cortex eucommiae is extracted, smashs to pieces, addition dregs of beans, rapeseed meal, corn flour, wheat
Bran is uniformly mixed；Zymocyte liquid is added in, is piled up at shady and cool drying and is compacted, sealing and fermenting is incorporated with plastic envelope two days later
In feedbag, sealing is compressed, anaerobic fermentation at shady and cool drying is placed in and forms for 14-16 days, the zymocyte liquid is brown sugar and plant
The aqueous solution of object lactobacillus+saccharomyces cerevisiae, gained fermented feed can not only improve livestock and poultry diseases prevention ability, but also can improve poultry
Poultry matter, but since zymocyte liquid combination is incomplete, it is impossible to a greater extent using the active ingredient in Cortex Eucommiae slag.
The content of the invention
Feed addictive it is an object of the invention to provide a kind of slag containing Cortex Eucommiae and preparation method thereof, the present invention is to Du
The Cortex Eucommiae dregs of a decoction after Zhong Ye extractions carry out secondary use, take full advantage of active ingredient contained in Cortex Eucommiae slag, are sent out by solid-state
The method of ferment makes in feed addictive rich in the enzymes such as amylase, acid protease, cellulase, pectase, alternative feed
Required complex enzyme formulation, and significantly improve the protein content in feed addictive.Feed addictive of the present invention is used
In layer breeding, laying rate of laying hen and egg size can be significantly improved.
The present invention is achieved through the following technical solutions：
The feed addictive of a kind of slag containing Cortex Eucommiae, including following raw material：Cortex Eucommiae slag, herba houttuyniae powder and Canton love-pea vine powder, Du
The weight ratio of secondary slag, herba houttuyniae powder and Canton love-pea vine powder is (4-5)：(1-2)： (1-2).
Preferably, the feed addictive of a kind of slag containing Cortex Eucommiae, including following raw material：Cortex Eucommiae slag, herba houttuyniae powder and chicken bone
Grass meal, the weight ratio of Cortex Eucommiae slag, herba houttuyniae powder and Canton love-pea vine powder is 5： 1：1.
Preferably, the feed addictive of a kind of slag containing Cortex Eucommiae, the Cortex Eucommiae slag are the Cortex Eucommiae dregs of a decoction after folium cortex eucommiae extraction.
Preferably, the preparation method of the feed addictive of a kind of slag containing Cortex Eucommiae, the herba houttuyniae powder and Canton love-pea vine powder is：
By cordate houttuynia and Canton love-pea vine chopping mashing, squeezed with squeezer, collect juice, it is dry, obtain powdered substance.
The present invention also provides a kind of preparation methods of the feed addictive of slag containing Cortex Eucommiae, comprise the following steps：It (1) will Du
Secondary slag, herba houttuyniae powder and the mixing of Canton love-pea vine powder, form mixture；(2) by step (1) made mixture and zymophyte, water, Portugal
Grape sugar is added in solid-state fermenter together to ferment；(3) fermentate obtained by step (2) is manufactured into that grain size is 0.5~1cm
Grain, it is dry, both feed addictive of the present invention.
Preferably, the preparation method of the feed addictive of a kind of slag containing Cortex Eucommiae, the zymogenic strain combination are：It is black
Aspergillus, bacillus subtilis, the inoculative proportion of saccharomyces cerevisiae are 1：1： 2；Zymogenic inoculum concentration is 2 × 106A/g.
Preferably, the preparation method of the feed addictive of a kind of slag containing Cortex Eucommiae, glucose additive amount in the step (2)
For the 2% of mixture quality, solid-liquid ratio is 1 in solid-state fermenter:2 (g/mL), fermentation condition are：Initial pH is 5, fermentation
35 DEG C of temperature, fermentation time 90h.
Aspergillus niger：Deuteromycotina, Hyphomycetes, hyphomycetales, Moniliaceae, a Common Species in aspergillus fungi.Extensively
In the general grain being distributed in all over the world, plant product and soil.Aspergillus niger can produce amylase, acid protease, fiber
Plain enzyme, pectase, glucose oxidase, citric acid, gluconic acid and gallic acid etc. can be provided greatly using fermentation of Aspergillus niger
Measure organized enzyme.
Bacillus subtilis：Bacillus subtilis is one kind of bacillus, is streptococcus pneumonia.Without pod membrane, Zhousheng
Flagellum can move.Gemma is in ellipticity or column, and central or slightly inclined positioned at thalline, thalline does not expand after sporulation.Bacterium colony
Rough surface is opaque, and when growing in liquid medium, wrinkle mould is commonly formed in dirty white or yellowish.Bacillus subtilis can
Using protein, a variety of sugar and starch, decompose tryptophan and form indoles.Bacillus subtilis can promote beneficial to the micro- life of anaerobism
The growth and breeding of object maintains intestinal eubiosis, can effectively prevent animal diarrhea and bacillary dysentery.
Cortex Eucommiae：Also known as bakelite, it is Eucommiaceae plant, contains that there are many amino acid, chlorogenic acid, vitamin, micro in folium cortex eucommiae
The several functions substance such as element, not only with spectrum antibacterial, raising animal body immunity and the speed of growth, improvement meat
The function of quality, also with the multi-efficiencies such as anti-inflammatory, antibacterial, antifatigue, anti-oxidant, hypoglycemic, enhancing immune function.
Cordate houttuynia：Cordate houttuynia is the herbal medicine that Chinese Pharmacopoeia is included, and herbal medicine source is the dry ground of saururaceae plant houttuynia cordata
Upper part.Cordate houttuynia contains volatile oil about 0.005%, wherein main antibacterial material is decanoyl acetaldehyde, meridian distribution of property and flavor is pungent, is slightly cold,
It is distributed in lung channel.Its primary efficacy is clearing heat and detoxicating, inducing diuresis for removing edema.Decanoyl acetaldehyde has a broad antifungal spectrum in herba houttuyniae powder, to pneumonia streptococcus
Bacterium, staphylococcus aureus (including Penicillin-resistant bacterial strain), Diplococcus pneumopniae, the inhibitory action of hemolytic streptococcus are strong,
It is weaker to micrococcus catarrhalis effect, it is especially stronger to the inhibitory action of Bacillus influenzae, typhoid bacillus to Gram-negative bacteria, to certain
A little pathogenic funguses also have certain inhibitory action, and the immunity of animal can be enhanced by being added to feed.
Canton love-pea vine：Canton love-pea vine is the drying complete stool of legume abrus cantoniensis Hance.Sweet, slight bitter, it is cool.There is removing dampness through diuresis and removing jaundice, clearly
The effect of thermal detoxification, dispersing the depressed liver-qi and alleviating pain.Canton love-pea vine plays an important role of to remove free radical and inhibits nitrosation, has protection to liver
Effect, the immunity of animal can be enhanced by being added to feed.
Beneficial effects of the present invention：
(1) in a kind of feed addictive of the slag containing Cortex Eucommiae of the present invention, the Cortex Eucommiae dregs of a decoction after being extracted to folium cortex eucommiae carry out secondary
It utilizes, takes full advantage of chlorogenic acid contained in Cortex Eucommiae slag, flavones, amino acid isoreactivity ingredient, make feed addictive of the present invention
Play an important role of that anti-inflammatory is antibacterial.
(2) a kind of feed addictive of the slag containing Cortex Eucommiae of the present invention is compared Cortex Eucommiae slag, herba houttuyniae powder and Canton love-pea vine powder as certain
Example mixed fermentation so that feed addictive of the present invention, can rich in enzymes such as amylase, acid protease, cellulase, pectases
The complex enzyme formulation needed for feed is substituted, and improves feed addictive protein content.
(3) feed addictive of the slag of the invention containing Cortex Eucommiae can not only enhance body's immunity, effectively facilitate animal life
It is long, efficiency of feed utilization is improved, and drug resistance is not generated, it is a kind of green, the functional feedstuff additive of safety.By the present invention
Feed addictive is used for layer breeding, can significantly improve laying rate of laying hen and egg size.
Specific embodiment
Embodiment 1
The feed addictive of a kind of slag containing Cortex Eucommiae, including following raw material：Cortex Eucommiae slag, herba houttuyniae powder and Canton love-pea vine powder, Du
The weight ratio of secondary slag, herba houttuyniae powder and Canton love-pea vine powder is 4：1：2.
Cortex Eucommiae slag is the Cortex Eucommiae dregs of a decoction after folium cortex eucommiae extraction in the feed addictive of the above-mentioned slag containing Cortex Eucommiae.
The preparation method of herba houttuyniae powder and Canton love-pea vine powder is in the feed addictive of the above-mentioned slag containing Cortex Eucommiae：By cordate houttuynia and
Canton love-pea vine chopping mashing, is squeezed with squeezer, collects juice, dry, obtains powdered substance.
A kind of preparation method of the feed addictive of slag containing Cortex Eucommiae, comprises the following steps：
(1) it is 4 by weight by Cortex Eucommiae slag, herba houttuyniae powder and Canton love-pea vine powder:1:2 are mixed, and form mixture；
(2) mixture in step (1) is taken to be added in together with zymophyte, water, glucose in solid-state fermenter, solid-state hair
Solid-liquid ratio is 1 in fermentation tank:2 (g/mL), glucose additive amount are the 2% of said mixture quality, and temperature is adjusted to 35 DEG C, PH
Initial value is adjusted to 5, wherein zymogenic strain combination is：Aspergillus niger, bacillus subtilis, the inoculative proportion of saccharomyces cerevisiae
For 1：1：2；Zymogenic inoculum concentration is 2 × 106A/g, ferment 90h；
(3) fermentate obtained by step (2) is manufactured into the particle that grain size is 0.5cm, it is dry, both it is of the present invention
Feed addictive.
Embodiment 2
The feed addictive of a kind of slag containing Cortex Eucommiae, including following raw material：Cortex Eucommiae slag, herba houttuyniae powder and Canton love-pea vine powder, Du
The weight ratio of secondary slag, herba houttuyniae powder and Canton love-pea vine powder is 5：1：1.
Cortex Eucommiae slag is the Cortex Eucommiae dregs of a decoction after folium cortex eucommiae extraction in the feed addictive of the above-mentioned slag containing Cortex Eucommiae.
The preparation method of herba houttuyniae powder and Canton love-pea vine powder is in the feed addictive of the above-mentioned slag containing Cortex Eucommiae：By cordate houttuynia and
Canton love-pea vine chopping mashing, is squeezed with squeezer, collects juice, dry, obtains powdered substance.
A kind of feed addictive preparation method of slag containing Cortex Eucommiae comprises the following steps：
(1) it is 5 by Cortex Eucommiae slag, herba houttuyniae powder and Canton love-pea vine powder weight ratio:1:1 is mixed, and forms mixture；
(2) mixture in step (1) is taken to be added in together with zymophyte, water, glucose in solid-state fermenter, solid-state hair
Solid-liquid ratio is 1 in fermentation tank:2 (g/mL), glucose additive amount are the 2% of said mixture quality, and temperature is adjusted to 35 DEG C, PH
Initial value is adjusted to 5, wherein zymogenic strain combination is：Aspergillus niger, bacillus subtilis, the inoculative proportion of saccharomyces cerevisiae
For 1：1：2；Zymogenic inoculum concentration is 2 × 106A/g, ferment 90h；
(3) fermentate obtained by step (2) is manufactured into the particle that grain size is 0.8cm, it is dry, both it is of the present invention
Feed addictive.
Embodiment 3
The feed addictive of a kind of slag containing Cortex Eucommiae, including following raw material：Cortex Eucommiae slag, herba houttuyniae powder and Canton love-pea vine powder, Du
The weight ratio of secondary slag, herba houttuyniae powder and Canton love-pea vine powder is 5：1：2.
Cortex Eucommiae slag is the Cortex Eucommiae dregs of a decoction after folium cortex eucommiae extraction in the feed addictive of the above-mentioned slag containing Cortex Eucommiae.
The preparation method of herba houttuyniae powder and Canton love-pea vine powder is in the feed addictive of the above-mentioned slag containing Cortex Eucommiae：By cordate houttuynia and
Canton love-pea vine chopping mashing, is squeezed with squeezer, collects juice, dry, obtains powdered substance.
A kind of feed addictive preparation method of slag containing Cortex Eucommiae comprises the following steps：
(1) it is 5 by Cortex Eucommiae slag, herba houttuyniae powder and Canton love-pea vine powder weight ratio:1:2 are mixed, and form mixture；
(2) mixture in step (1) is taken to be added in together with zymophyte, water, glucose in solid-state fermenter, solid-state hair
Solid-liquid ratio is 1 in fermentation tank:2 (g/mL), glucose additive amount are the 2% of said mixture quality, and temperature is adjusted to 35 DEG C, PH
Initial value is adjusted to 5, wherein zymogenic strain combination is：Aspergillus niger, bacillus subtilis, the inoculative proportion of saccharomyces cerevisiae
For 1：1：2；Zymogenic inoculum concentration is 2 × 106A/g, ferment 90h；
(3) fermentate obtained by step (2) is manufactured into the particle that grain size is 1cm, it is dry, both feeding of the present invention
Feed additives.
Comparative example 1：With differing only in for embodiment 1, in feed addictive without addition Cortex Eucommiae slag, other conditions and
Preparation method is same as Example 1.
Comparative example 2：With differing only in for embodiment 1, no addition Canton love-pea vine powder, other conditions in feed addictive
It is same as Example 1 with preparation method.
Comparative example 3：With differing only in for embodiment 1, no addition herba houttuyniae powder, other conditions in feed addictive
It is same as Example 1 with preparation method.
Comparative example 4：With differing only in for embodiment 1, no addition herba houttuyniae powder and Canton love-pea vine in feed addictive
Powder, other conditions and preparation method are same as Example 1.
Application Example 1
Bacteriostatic test
Experiment material：The feed addictive of the slag containing Cortex Eucommiae, beef extract-peptone.
For trying bacterium：Staphylococcus glucose bacterium, streptococcus pneumonia, Escherichia coli.
Minimum inhibitory concentration (MIC) determines：Mixed plate dilution method
The preparation of bacteria suspension：Staphylococcus glucose bacterium is taken to be inoculated on beef-protein medium inclined-plane, it is living at 37 DEG C
Change culture for 24 hours, choose bacterium from inclined-plane with oese and suspension is made into 100mL sterile waters, then make 10 times of dilutions, and lead to
Overmulling plate count determines the dilution factor of bacterium solution, makes last bacteria suspension institute bacteria containing amount in 1x106~1x107cfu/mL。
The feed addictive solution allocation of the slag containing Cortex Eucommiae：The feed addictive 40mg of the slag containing Cortex Eucommiae in embodiment 1 is weighed,
Add sterile pure water 1mL, be configured to the feed addictive solution of the slag containing Cortex Eucommiae of mass concentration 40mg/mL, ice-bath ultrasonic 40min.
After the feed addictive solution of the slag containing Cortex Eucommiae is diluted to 1000 μ g/mL of mass concentration with sterile pure water, then to being serially diluted again
Into various concentration by test solution, i.e. 500.00 μ g/mL, 250.00 μ g/mL, 125.00 μ g/mL, 62.50 μ g/mL, 31.25 μ g/
mL。
Under inoculated and cultured sterile working, the feed addition of 10mL bacterium solutions and the 10mL slag containing Cortex Eucommiae is drawn respectively with liquid getting device
Agent solution is injected into the small centrifuge tube of sterilizing, and after abundant mixing, rapid 200 μ L mixed liquors of drawing are injected into sterilizing plates,
Do two it is parallel, blank is made with the mixed liquor of 10mL bacterium solutions and the sterile water of same volume, can also be directly injected into 100 μ L of bacterium solution work
Blank；Pour into 10~12mL of culture medium that is sterilized, being placed in constant temperature at 45 DEG C (culture medium pouring volumes in experiment plate every time
Should be consistent) in, rocking plate makes mixed liquor fully scatter, and is evenly distributed on plate, after culture medium solidification, is placed in
1~2d is cultivated in 37 DEG C of incubators, colony growth situation is observed, and bacterium colony is counted.
Bacteriostasis rate=(blank control clump count-sample treatment clump count)/blank control clump count × 100%.
Table 1
To the minimum inhibitory concentration (MIC) of staphylococcus aureus
Table 2
To the minimum inhibitory concentration (MIC) of streptococcus pneumonia
Table 3
To the minimum inhibitory concentration (MIC) of Escherichia coli
By table 1, table 2, table 3 as it can be seen that a kind of feed of slag containing Cortex Eucommiae of the present invention is to the minimum inhibitory concentration for supplying examination bacterium
400μg/mL.Meanwhile with the increase of feed addictive concentration, to also increasing for the bacteriostasis rate of examination bacterium, when feed addictive
When concentration has reached 500 μ g/mL, 99.5% is reached to the bacteriostasis rate of staphylococcus aureus, to the antibacterial of streptococcus pneumonia
Rate has reached 99.5%, has reached 99.5% to the bacteriostasis rate of Escherichia coli.By this experiment as it can be seen that one kind of the present invention is containing Du
The feed of secondary slag has good scavenging action to staphylococcus aureus, streptococcus pneumonia, Escherichia coli.
Application Example 2
Measure the feed addictive protein content of the slag containing Cortex Eucommiae
Reagent and equipment
Reagent：The concentrated sulfuric acid；30% hydrogenperoxide steam generator；10M sodium hydroxides；0.01M standard hydrochloric acid；Standard ammonium sulfate
(0.3mg nitrogen/mL)；Catalyst：Copper sulphate：Potassium sulfate=1：4 mixing, it is finely ground.
Indicator：0.1% methyl red ethanol solution.
Equipment：Micro kjeldahl apparatus；Pipette 1mL；Pipette 2mL；Microburet 5mL beakers 200mL；Graduated cylinder
10mL；Conical flask 150mL；Kjeldahl flask 50mL, rubber pipette bulb；Electric furnace；Assay balance.
Test sample：1~4 made feed addictive of Examples 1 to 3 and comparative example
Test method：
1~4 each 50mg of made feed addictive of Examples 1 to 3 and comparative example is weighed, is separately added into 14 kelvin beakers
In, another 2 kelvin beakers are blank control, are not added with sample.Respectively about 500mg potassium sulfates-sulphur is added in each beginning beaker
Sour copper mixture, then add the 5mL concentrated sulfuric acids.
Above 16 kelvin beakers are heated, treats that water in bottle decatize is complete, suitably increases firepower, liquid in bottle is made to boil slightly
It rises, maintains 2~3h.After digestive juice becomes brown, beaker is removed, it is slightly cold, 30% hydrogenperoxide steam generator 1~2 is added drop-wise to
In beaker, becoming pale blue green until digestive juice is again faint yellow, cooling pours into liquid in bottle in 50mL volumetric flasks, and to steam
Distilled water washs flask several times, washing lotion is incorporated in volumetric flask, constant volume is spare.
Take 50mL conical flasks several, respectively plus 25mL0.01M standards HCL and 1~2 drips indicator, is covered with surface plate standby
With.2mL is taken to dilute, reative cell is added in by small funnel.One conical flask equipped with 0.01M standards HCL and indicator is placed on cold
Under solidifying pipe, condenser pipe lower end is made to be immersed in liquid.
5mL10M sodium hydroxides are taken, small funnel is poured into, sodium hydroxide is allowed to flow slowly into reative cell, when not flowing to end completely
Step up clip, 5mL distilled water is added in into small funnel, reative cell is slowly put into, a small amount of water is stayed to make water seal in funnel.Heating
Vapor reactor closes collector piston after boiling.It treats that ammonia distillation is complete, conical flask is removed, with 0.01M sodium hydroxide marks
Quasi- solution titration, writes down consumed volume.
X is sample protein matter content (g/100g), and A is the sodium hydroxide mL numbers that titration blank is spent, and B is titration sample
The sodium hydroxide mL numbers spent, c are standard HCL concentration, and M is the quality of sample, and F is the coefficient that nitrogen is scaled protein, wherein
C=0.01M, F=5.7.
Feed addictive protein content is shown in Table 4
Table 4
Comparative example 1~4 is significantly higher than by the protein content of 4 visible Examples 1 to 3 of table.It can be seen that the present invention is a kind of
The feed addictive protein content of the slag containing Cortex Eucommiae is significantly improved.
Application Example 3
A kind of application test of the feed addictive of slag containing Cortex Eucommiae of the present invention in egg feedstuff.
A kind of feed addictive of slag containing Cortex Eucommiae of the 50g present invention is added in every kilogram of basic egg feedstuff.
160 healthy egg-laying peak laying hens are selected, are randomly divided into 8 processing groups, laying hen is 20 in each processing group
Only.It is one~experimental group of experimental group three to number respectively, one~contrast groups of contrast groups five.
The egg feedstuff of the feed addictive of one laying hen feeding of the experimental group addition 1 made slag containing Cortex Eucommiae of embodiment.
The egg feedstuff of the feed addictive of two laying hen feeding of the experimental group addition 2 made slag containing Cortex Eucommiae of embodiment.
The egg feedstuff of the feed addictive of three laying hen feeding of the experimental group addition 3 made slag containing Cortex Eucommiae of embodiment.
The egg feedstuff of the feed addictive of one laying hen feeding of the contrast groups addition 1 made slag containing Cortex Eucommiae of comparative example.
The egg feedstuff of the feed addictive of two laying hen feeding of the contrast groups addition 2 made slag containing Cortex Eucommiae of comparative example.
The egg feedstuff of the feed addictive of three laying hen feeding of the contrast groups addition 3 made slag containing Cortex Eucommiae of comparative example.
The egg feedstuff of the feed addictive of four laying hen feeding of the contrast groups addition 4 made slag containing Cortex Eucommiae of comparative example.
Five laying hen feeding of contrast groups is not added with the basic egg feedstuff of the feed addictive of the slag containing Cortex Eucommiae.
One month each experimental group of separate collection and contrast groups laying hen descend egg, and calculate average egg weight.
Experimental group one~tri-, one~five egg production of contrast groups and egg always see the table below 5
Table 5
By table 5 as it can be seen that the egg production and egg size of one~experimental group of experimental group three are significantly higher than contrast groups 1~5, thus may be used
When seeing that a kind of feed addictive of the slag containing Cortex Eucommiae of the present invention is applied to egg feedstuff, can significantly improve laying hen laying rate and
Egg size.
Above-described embodiment only illustrates embodiment of the present invention, and there is no the limitations having to the present invention in any form；Appoint
What is engaged in professional and technical personnel, all has the ability to make some changes or modification using the technology contents disclosed, becomes equivalent
The equivalent embodiment of variation, technical spirit, carries out simple modification, equivalent change and modification to the present invention, belongs to according to the present invention
In the range of technical solution of the present invention.
Claims (7)
1. the feed addictive of a kind of slag containing Cortex Eucommiae, which is characterized in that including following raw material：Cortex Eucommiae slag, herba houttuyniae powder and chicken
Bone grass meal, the weight ratio of Cortex Eucommiae slag, herba houttuyniae powder and Canton love-pea vine powder is (4-5)：(1-2)：(1-2).
2. the feed addictive of a kind of slag containing Cortex Eucommiae according to claim 1, which is characterized in that including following raw material：
Cortex Eucommiae slag, herba houttuyniae powder and Canton love-pea vine powder, the weight ratio of Cortex Eucommiae slag, herba houttuyniae powder and Canton love-pea vine powder is 5：1：1.
3. the feed addictive of a kind of slag containing Cortex Eucommiae according to claim 2, which is characterized in that the Cortex Eucommiae slag is Cortex Eucommiae
The Cortex Eucommiae dregs of a decoction after leaf extraction.
4. the feed addictive of a kind of slag containing Cortex Eucommiae according to claim 2, which is characterized in that the herba houttuyniae powder and chicken
The preparation method of bone grass meal is：Cordate houttuynia and Canton love-pea vine are shredded into mashing respectively, squeezed with squeezer, collects juice, it is dry, point
Powdered substance is not obtained.
5. a kind of a kind of preparation method of the feed addictive of slag containing Cortex Eucommiae as described in claim any one of 1-4, including following
Step：(1) Cortex Eucommiae slag, herba houttuyniae powder and Canton love-pea vine powder are mixed, forms mixture；(2) by step (1) made mixture and hair
Yeast-like fungi, water, glucose are added in solid-state fermenter together to ferment；(3) fermentate obtained by step (2) is manufactured into grain size as 0.5
The particle of~1cm, it is dry, both feed addictive of the present invention.
A kind of 6. preparation method of the feed addictive of slag containing Cortex Eucommiae according to claim 5, which is characterized in that the step
(2) zymogenic strain combination is：Aspergillus niger, bacillus subtilis, saccharomyces cerevisiae, aspergillus niger, bacillus subtilis, wine brewing ferment
Female inoculative proportion is 1：1：2；Zymogenic inoculum concentration is 2 × 106A/g.
A kind of 7. preparation method of the feed addictive of slag containing Cortex Eucommiae according to claim 5, which is characterized in that the step
Suddenly glucose additive amount is the 2% of mixture quality in (2), and solid-liquid ratio is 1 in solid-state fermenter:2 (g/mL), fermentation condition
For：Initial pH is 5,35 DEG C of fermentation temperature, fermentation time 90h.
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