A kind of waste matter of fermenting produces the apparatus and method for of high activity high nutrition feed
Technical field
The invention belongs to the preparing technical field of feed, and in particular to one kind fermentation waste matter production high activity high nutrition feed
Equipment, the invention further relates to it is a kind of ferment waste matter produce high activity high nutrition feed method.
Background technology
China is the first in the world herding and aquaculture big country, and national meat total output is more than 80,000,000 tons, birds, beasts and eggs yield
Up to 30,000,000 tons, 32,900,000 tons of milk yield, aquatic products annual production is up to 51,000,000 tons;By intensive culture feed efficiency point
Wei 5:1 (meat), 3:1 (egg), 1:3 (milk) and 2:1 (aquaculture product) is calculated, and national year consumption concentrated feed is 7980*5+
Hundred million tons of 2940*3+3290/3+5100*70%*2=5.7, in theory the grain grease type resource equivalent to China be essentially available for
Aquaculture.This be China about 1,000,000 tons of fish meal of annual import, 50,000,000 tons of dregs of beans (or soybean), 10,000,000 tons of corns base
On plinth, traditional cultural method is carried forward largely using the development aquaculture such as stalk, waste matter, forage, makes the lance between grain and feed
Shield reaches balance, and cracks the prophesy of " Chinese grain supports the Chinese that are unlikely to survive " that American says.
China's waste matter resource very abundant, often produces about 60,000,000 tons of grouts, more than 100,000,000 tons of poor slag per year;With food and agriculture
The development of Product processing industry, the byproduct grouts of all kinds of oil seed productions and food, light industry, Chinese patent drug processing the poor slag of byproduct by
Year increase.The Land use systems of the current poor slag in various regions and grouts inferior are as feed, fertilizer and fuel etc..It is to meet as feed
The most scientific application process of China's national situation (resource scarcity of feed grain);But the crude fiber content of poor slag and grouts inferior is high, egg
White matter content is low, and effective energy value is low, and protein (amino acid) digestive utilization ratio is low;Have, grouts especially inferior, also containing anti-
The toxic components such as trophic factors, it is impossible to or can not feeding animals in large quantities.China produces 1.8 hundred million tons of mixed feed per year in recent years, with
The propulsion of modern cultural technique, the ratio of mixed feed will increase year by year, and the supply of feedstuff turns into restriction China feed work
The bottleneck of industry and aquaculture development.The fundamental solution solved the problems, such as is the exploitation of waste matter.The feed of the current poor slag of China
Industrial utilization is less than 10%, and the feed industry of grouts inferior utilizes about 30%;Therefore, the feed development of waste matter utilizes the market space
Have a high potential.
Improve waste matter quality at present, the most common method for improving nutritive value is biofermentation, and fermented bacterium is mainly
Bacillus (bacillus subtilis, bacillus licheniformis, bafillus natto), saccharomycete (candida tropicalis, Candida utilis ferment
Female and saccharomyces cerevisiae, geotrichum candidum), mould (aspergillus, trichoderma, head mold etc.) and lactic acid bacteria etc..Wherein fungal cellulases are higher, have
Help the degraded of cellulose;Brood cell bacterium carbohydrase, protease equal size are higher, contribute to macromolecular sugar, the degraded of protein, carry
High digestibility;Saccharomycete thalline is big, protein content is high, sugar and nitrogen synthesising thalli protein can be made into protein in tunning
Content improve, quality improving;Lactic acid bacteria produces the acids such as substantial amounts of lactic acid, is conducive to the fresh-keeping of fermented feed and improvement
Palatability.The reasonable combination fermentation of these strains, can be improved the feed nutritive value of waste matter, while providing benefit for animal
Raw bacterium and digestive ferment, are conducive to the disease-resistant growth-promoting of animal.General use single culture or composite bacteria on fermentation process
Solid fermentation method, aerobic and anaerobism, one time fermentation or secondary fermentation;The ununified standard of Zymolysis Equipment, mostly barreled or pond
Fill (stir or do not stir) fermentation, a small number of solid-state fermentation tank fermentations.
Edible mushroom is the nutraceutical of high-quality, but the fructification production cycle is long, yields poorly, and carries out mycelium extensively in recent years
Fermenting and producing health food and health medicine.Mycelium protein matter content height (20-50%), nutriment enriches, and grows numerous
Grow fast, enzyme activity is high, active polysaccharide polypeptide can be produced with the lignocellulosic in decomposition and inversion culture matrix and macromolecule protein etc.
And other functional mass;Therefore protein content in feed can both have been improved with its roughage of fermenting, the immunity of raising animal (subtracts
Lack disease) and the speed of growth (increasing economic efficiency), the quality of meat can also be improved.But with edible fungus fermented waste matter etc.
Roughage, especially the research report with other fungies and bacterium mixed fermentive are less;Invention edible fungus of the invention and life
The agricultural byproducts such as the strong other strain combined fermentation waste matters of complementary activities are managed, high-activity biological feed is produced, to improve very
Advise the utilization rate of feed.
There is problems with existing fermentation:1st, the problem of fermented bacterium:Current feed bio-fermentation agent is special due to strain
Property limitation (such as lactic acid bacteria production acid too high, saccharomyces cerevisiae production alcohol is too high, the biological enzyme activity of Bacillus is low, mold fermentation is easy
The harmful bacterias such as aspergillus flavus mix) or strain between combination matching it is improper etc.;During the poor slag of fermentation, except being formed
Outside more active probiotic, organic acid, alcohol and biology enzyme, also there is certain degraded to the ANFs of grouts, but to grain
Macromolecular substances in the dregs of rice, especially cellulose degradation rate are very low, unfavorable nonruminant digestion;Meanwhile, fermentation temperature is high (35 DEG C
More than), energy loss is big;Also it is easy to miscellaneous bacteria (being especially harmful to mould and spoilage organisms) pollution, feed, which goes rotten, to go bad, and causes dynamic
Thing is poisoned.If the fresh feeding of fermented feed often occurs because the production such as lactic acid bacteria is organic, hyper acid, causes animal body acid-base imbalance;Or wine brewing
The concentration of yeast production alcohol is big, causes animal alcoholism.If feedstuff is made through drying in fermentation material, in the drying process
The volatile materials such as substantial amounts of low molecular organic acidses, alcohol are vapored away with heat, cause the serious damage of nutriment, especially energy
Lose.2nd, the problem of zymotechnique:At present in the production technology of biological fermentation feed, one is to use anaerobic ferment process, that is, is used
The anaerobism such as lactic acid bacteria, Bacillus, yeast or amphimicrobian strain, waste matter is put in sealing container and fermented;With aerobic fermentation ratio, life
Thing reaction is slow, and efficiency is low, and the effect of fermentation is poor.Two be that with aerobic bacterias such as moulds, waste matter is put into unlimited fermentation bed or fermentation vat
Middle progress aeration type fermentation;Its fermentation efficiency is greatly improved, but condition is difficult to control to, and easy living contaminants, effect is unstable.Three
It is the aerobic fermentation that the short time is first carried out with aerobic bacteria, then secondary anaerobic fermentation is carried out with anaerobic bacteria;But because of complex process, in life
Using less in production.3rd, Zymolysis Equipment device problem:Waste matter fermentation is solid fermentation, and existing solid fermentation apparatus removes traditional hair
Outside ferment bucket and fermentation vat, having shallow tray fermentation reactor, (mass-and heat-transfer speed is low, causes high pollution risk and pallet utilization rate low
Shortcoming), fluid bed fermentation reactor (reactor volume rate is low), (material lumps and glues wall, reactor rotary drum fermentation reactor
The problem of plot ratio is low), disc type fermentation reactor (being unable to sterile working), stirring-type fermentation reactor (be only applicable to small rule
Mould ferments), air pressure oscillation fermenter reactor (equipment requirement is high, and material prepares difficulty etc.);Also have been reported that box type solid material
Zymolysis Equipment (application number:200420026272.0), the equipment is designed as ladder conveyer belt type, and floor space is big and grows, and is applicable
Property is poor.In summary, in various solid-state reactors, what is be industrially applied also only has disc type, rotary drum and stirring-type
Reactor, the domestic development to various performance solid-state reactors has also just started.Due to the Product management model of reactor must be solved,
Prevent fermentation process from polluting, and a series of problems, such as process monitoring, when considering development of new solid-state fermentation reactor, force logical
Wind, temperature control, material should not remain static for a long time, can heat, and mechanization degree is high, it is easy to operates, is easy to cleaning and sterilizing,
The factors such as small investment are crucial.Up to the present there is not yet relevant industrially scalable solid-state fermentation reactor product, feed hair
Ferment with rotary drum or stirred-tank fermenter in addition to strain is produced, and commodity production is fermented using fermentation vat (groove), fermenter mostly,
Stir or do not stir in fermentation, fermentation materials not sterilization, fermentation process is difficult to control to, and easily contaminates miscellaneous bacteria, product quality is not
It is stable;Zymolysis Equipment influences bio-fermentation process and effect, is the bottleneck for restricting solid fermentation industrial development.
The content of the invention
To overcome the shortcomings of above mentioned problem, the present invention provides a kind of setting for waste matter production high activity high nutrition feed that ferments
It is standby.
It is a further object of the present invention to provide a kind of method that waste matter of fermenting produces high activity high nutrition feed.
First technical scheme of the present invention is, the equipment that a kind of waste matter of fermenting produces high activity high nutrition feed,
Including casing, the inside top of casing is provided with vent window, atomizer mechanism and ultraviolet lamp tube, and the top outer of casing is provided with
Cistern, mixing bunker, the top of mixing bunker are provided with cooling zone, and cooling zone is connected with microwave sterilization conveyer, mixing bunker
Portion is disposed with mixer and is connected with fermentation bed mechanism from top to bottom, and fermentation bed mechanism is arranged in casing;The bottom of casing
Portion is provided with ventilation input pipe and electric heater, and ventilation input pipe is connected with air cleaner, air conditioner in turn, and (summer temperature is high
Shi Jiangwen use) and air blower, several air outlets are provided with ventilation input pipe;Electric heater is at least provided with 2, electrical heating
Device is arranged at the both sides of ventilation input pipe;The side of casing and discharging window is provided with below mixer, is set at discharging window
There is the 3rd conveyer belt of movable type;Another of casing is provided with maintenance door sideways;Another of casing is provided with ozone sideways
Into pipe, ozone connects into pipe with ozone generator, is provided with outside casing in switch board, switch board and is provided with temperature humidity sense
Answer control module.
In the above-mentioned technical solutions, fermentation bed mechanism includes the first fermentation bed and the second fermentation bed, the first fermentation bed and the
By first conveyer belt and the second conveyer belt between two fermentation beds, one end of first conveyer belt is arranged at the first fermentation bed bottom,
The other end of first conveyer belt is arranged at the top of the second fermentation bed, and one end of the second conveyer belt is arranged on the bottom of the second fermentation bed
Portion, the other end of the second conveyer belt is arranged at the top of the first fermentation bed.
First fermentation bed and the second fermentation bed are provided with 3-7 layers of fermentation crawler belt layer, length 1-10m or more;Every layer of hair
Ferment crawler belt layer is rotated by motor or speed changer regulation;Speed changer electric wire connects with the switch board case outside case.
Atomizer mechanism includes being provided with several shower nozzles in mist pipe, mist pipe.
Vent window and ultraviolet lamp tube are at least provided with 2.
Temperature humidity sensing control module includes single-chip microcomputer, and single-chip microcomputer is connected with temperature sensor by wire respectively, wet
Spend sensor, electric heater and atomizer mechanism.
Second technical scheme of the present invention is, a kind of method that waste matter of fermenting produces high activity high nutrition feed,
The equipment that high activity high nutrition feed is produced using the fermentation waste matter described in the claims, is comprised the following steps:
Step 1, from edible fungus, fodder yeast, mould or prebiotic Bacillus, prepare liquid spawn, solid spawn or
Person's composite bacteria;
Step 2, waste matter fermentation process is carried out to the above-mentioned strain for preparing;
Step 3, the waste matter fermented is dried and crushed, prepare feedstuff product.
In the above-mentioned technical solutions, liquid spawn specifically prepares as follows in step 1:
1), prepared by edible fungus species:By in the hypha of edible fungus inoculation comprehensive PDA culture medium activated by flat board, it is trained
Foster condition is:15 DEG C -28 DEG C of cultivation temperature, shaking speed 60-180r/min, incubation time 48-96h;
2), prepared by mold species:By the inoculation Cha Shi trainings of the filamentous fungis such as the aspergillus activated through flat board, head mold, trichoderma, mould
Support in base, its condition of culture is:20 DEG C -28 DEG C of cultivation temperature, shaking speed 100-180r/min, incubation time 24-48h;
3), prepared by yeast seeds:By yeast such as the candida utili, saccharomyces cerevisiae, the geotrichum candidums that are activated by flat board
In strain inoculation malt extract medium, its condition of culture is:20 DEG C -32 DEG C of cultivation temperature, shaking speed 120-180r/min, training
Support time 12-36h;
4), prepared by Bacillus:By in the Bacillus inoculation nutrient broth medium activated by flat board, it is trained
Foster condition is:25 DEG C -37 DEG C of cultivation temperature, shaking speed 120-180r/min, incubation time 12-24h;
Solid spawn specifically prepares as follows:
1) prepared by edible fungus species:By the edible bacterium solution such as the flat mushroom prepared, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius
Body seed accesses solid medium by 5%-20% inoculum concentrations, and (solid medium is:20-40% corn flour, 30-50% wheat brans,
15-25% rice brans, 5-15% dregs of beans, 0.1%MgSO4, 0.1%KH2PO4) in, 15 DEG C -28 DEG C, quiescent culture time 48-96h,
Mycelial density reaches ++++(mycelia is covered with);
2) prepared by mold species:By the filamentous fungi liquid seeds such as the aspergillus prepared, head mold, trichoderma, mould by 2%-
15% inoculum concentration accesses solid medium, and culture medium is with edible mushroom seed culture medium, and 20 DEG C of -28 DEG C of culture 24-48h, mycelia is close
Degree reaches ++++(mycelia is covered with);
3) prepared by yeast seeds:By the candida utili prepared, button capsule laminating adhesive yeast, saccharomyces cerevisiae, geotrichum candidum
It is linked into Deng fodder yeast bacteria liquid seed by 1%-10% in solid medium, culture medium is with edible mushroom seed culture medium, 20
DEG C of -32 DEG C culture 12-24h, inoculum density is up to 1 × 1010More than cfu/g;
4) prepared by Bacillus:By the bacillus subtilis prepared, bacillus licheniformis, natto gemma bar gemma
Bacteria liquid seed is linked into solid fermentation culture medium by 1%-5%, and solid medium is:20-50% wheat brans, 20-40% meter
Chaff, 20-40% dregs of beans, 10-30% corn flour, 1% glucose, 0.5% (NH4)2SO4, 0.1%MgSO4, 0.3%K2HPO4、
0.15KH2PO4, 0.5%NaCl, 0.01%FeSO4·H2O, 25 DEG C -37 DEG C, cultivates 12-24h, strain density is up to 1 × 1011cfu/g
More than;
Composite bacteria is specifically prepared as follows:Cultured solid spawn or liquid spawn press edible mushroom or mould
Bacterium:Saccharomycete:Bacillus=3-10:1-3:0.5-2 mass ratio is with being mixed and made into composite bacteria or singly deposit structure combination strain
Kind.
Carrying out fermentation process to the above-mentioned strain prepared in step 2 is specially:
Step 2.1, preparation fermentation base-material:The base-material that ferments includes poor slag compost, grouts compost;
Step 2.2, sterilization:By the directly application of free of contamination fermentation base-material prepare, fresh or sterilized go out
Bacterium;The equipment of waste matter of fermenting production high activity high nutrition feed carries out sealing sterilization before charging fermentation with ultraviolet and ozone
More than 2h;
Step 2.3, inoculation and fermentation:Fermentation base-material to be fermented once admixes 5%-20% complex solids strain or liquid
Strain, or by growth speed, strain is combined in inoculation by several times, and specially growth is slow is first connect, and growth is fast to be followed by, between the time
Every 12-24h, stir, fill tray, expect thickness 5-15cm, lead under the conditions of 20-35 DEG C of temperature, relative humidity 65-100%
Gas is fermented, and 1-12h is stirred once, or continuous driving and conveying belt stirring at a slow speed, and ferment 36-96h, treats to cover with white bacterium on compost
Body is degree.
The waste matter fermented is dried and crushed in step 3, preparing feedstuff product is specially:Will fermentation
Good waste matter feed is using 50-85 DEG C of instantaneous quick drying apparatus;Dried fermentation waste matter carries out being crushed to 20-80 mesh, wraps
Dress, i.e. feedstuff product, are produced for mixed feed;Feedstuff product is divided into feature energy feed and functional protein
Feed:Poor slag fermentation protein content is feature energy feed below 25%;Grouts ferment crude protein content 25% with
On, it is functional protein feed.
The beneficial effects of the invention are as follows:With that existing bacteria combination, bacterium combine etc. with yeast and filamentous fungi is half logical
Difference between aerobe fermentation ratio, composite bacteria of the present invention, strain is big, and respective advantage is protruded, with strong complementarity, and using whole oxygen supply hair
Ferment, fermentation is abundant, and fiber testing is high, and albumen increase rate is big, and active material (fungi polysaccharide, polypeptide etc.) content is high, and fermentation
Temperature is low, and energy loss is few;The equipment of the present invention meets technological requirement, than existing solid fermentation apparatus ratio:Interior environmental conditions
Control and (solve the technical bottleneck that solid biologic fermentation temperature, humidity and oxygen supply control difficult and fungal mycelium to stir),
The sterilizing and bacterial system of low energy consumption, can meet the requirement of production, and integral device automaticity is high, is particularly suitable for extensive life
Production, saving of work and time, efficiency high.(existing fermenter, pond or groove, equipment are simple, but the control of fermentation condition is difficult, ferment effect
Difference;Existing solid fermentation can system, it is expensive, and the condition control effect such as temperature, humidity of fermentation is poor, and it is inadaptable greatly
Large-scale production).
Brief description of the drawings
Fig. 1 is the structural representation of the equipment of present invention fermentation waste matter production high activity high nutrition feed;
Fig. 2 is the structural representation of fermentation bed mechanism of the present invention;
Fig. 3 is that the temperature humidity of the present invention senses the structural representation of control module.
In figure, 1. culture materials, 2. microwave sterilization conveyers, 3. mixers, 4. cooling zones, 5. strains add mouth, and 6. lead to
Air regulator, 7. cisterns, 8. atomizer mechanisms, 9. maintenance doors, 10. air cleaners, 11. air blowers or air-cooler, 12. electrical heating
Device, 13. ventilation input pipes, 14. ozone, which enter, manages, 15. ozone generators, 16. discharging windows, 17. the 3rd conveyer belts, and 18. is ultraviolet
Fluorescent tube, 19. humidity sensors, 20. casings, 21. mixing bunkers, 22. first fermentation beds, 23. first conveyer belts, 24. second fermentations
Bed, 25. fermentation bed mechanisms, 26. second conveyer belts, 27. temperature humidities sensing control module, 28. single-chip microcomputers, 29. TEMPs
Device, 30. fermentation crawler belt layers.
Embodiment
With reference to embodiment, the present invention is described in detail.
The present invention provides the equipment that a kind of waste matter of fermenting produces high activity high nutrition feed, as shown in figure 1, including casing
20, the inside top of casing 20 is provided with vent window 6, atomizer mechanism 8 and ultraviolet lamp tube 18, and the top outer of casing 20 is set
There are cistern 7, mixing bunker 21, the top of mixing bunker 21 is provided with cooling zone 4, and cooling zone 4 is connected with microwave sterilization conveyer 2,
The bottom of mixing bunker 21 is disposed with mixer 3 and is connected with fermentation bed mechanism 25 from top to bottom, and fermentation bed mechanism 25 is set
In in casing 20;The bottom of casing 20 is provided with ventilation input pipe 13 and electric heater 12, and ventilation input pipe 13 is connected with turn
Air cleaner 10, air blower or air-cooler 11 are (in air cleaner 10 and air blower or cold when summer temperature height needs to cool
Be provided between blower fan 11 air conditioner be used for cool), ventilation input pipe 13 on be provided with several air outlets;Electric heater 12 to
2 are provided with less, and electric heater 12 is arranged at the both sides of ventilation input pipe 13;The side of casing 20 and in the second fermentation bed 24
Bottom is provided with discharging window 16, discharging window 16 and is provided with the 3rd conveyer belt 17, and another of casing 20 is provided with maintenance sideways
Door 9;Another of casing 20 is provided with ozone into pipe 14 sideways, and ozone connects into pipe 14 with ozone generator 15, casing
Outer be provided with switch board, switch board is provided with temperature humidity sensing control module 27.
As shown in Fig. 2 fermentation bed mechanism 25 includes the first fermentation bed 22 and the second fermentation bed 24, the first fermentation bed 22 and the
Respectively by the conveyer belt 26 of first conveyer belt 23 and second between the two ends of two fermentation beds 24, one end of first conveyer belt 23 is set
In the bottom of the first fermentation bed 22, the other end of first conveyer belt 23 is arranged at the top of the second fermentation bed 24, the second conveyer belt 26
One end be arranged on the bottom of the second fermentation bed 24, the other end of the second conveyer belt 26 is arranged on the top of the first fermentation bed 22.
First fermentation bed 22 and the second fermentation bed 24 are provided with 3-7 layers of fermentation crawler belt layer 30, length 1-10m or more;Every layer of fermentation
Crawler belt layer is rotated by motor or speed changer regulation;Motor or speed changer electric wire connect with the switch board case outside case.
Atomizer mechanism 8 includes being provided with several shower nozzles in mist pipe, mist pipe.Vent window 6 and ultraviolet lamp tube 18 to
2 are provided with less.
As shown in figure 3, temperature humidity sensing control module 27 includes single-chip microcomputer 28, single-chip microcomputer 28 is connected by wire respectively
There are temperature sensor 29, humidity sensor 19, electric heater 12 and atomizer mechanism 8.
The equipment of fermentation waste matter production high activity high nutrition feed is by casing and Accessory Members, fermentation bed mechanism, sterilizing
Decontamination system, temperature humidity and air handling system, material transmission and the corollary equipments such as system composition of stirring:
First, casing and Accessory Members:Casing 20 is made by metal or other corrosion resistant insulation materials, its length, width
Degree is determined with height by the size of internal layer frame of fermenting with how many;Top sets vent window 6, atomizer sprayer 8 and ultraviolet lamp tube 18
Respectively about dry.The top of one end of casing 20 sets mouth under fermentation base-material and strain mixing bunker 21, storehouse and installs mixer 3, for bacterium
Plant the mixing with base-material.The bottom center of casing 20 longitudinally sets ventilation input pipe 13, is connected outside with air blower or air-cooler 11, leads to
Output about dry, air port in the both sides of wind input pipe 13.The bottom of casing 20 sets electric heater 12, for heating.The other end of casing 20
Middle part is provided with maintenance door 9, is easy to the maintenance of internal unit;Material is set out at moveable 3rd conveyer belt 17 in casing one end bottom
Window 16, the handling of material and the 3rd conveyer belt 17 after fermentation are spread out of for the 3rd conveyer belt 17.
Second, fermentation bed mechanism:Fermentation bed mechanism is divided to two groups (the first fermentation bed 22 and second fermentation beds 24), every group of 3-7
Layer (every layer of 30-50cm height) fermentation crawler belt layer 30, rubber, plastics or other materials system that fermentation crawler belt layer 30 is lost by heat-proof corrosion resistant
Into band edge conveyer belt make, bandwidth 0.5-5.0m or more, the high 5-15cm in side;In the one or both ends peace of fermentation crawler belt layer 30
Fill reducing motor, the rotation of driving fermentation crawler belt layer 30.Set and incline greatly between the two ends of first fermentation bed 22 and the second fermentation bed 24
Angle conveyer belt (conveyer belt 26 of first conveyer belt 23 and second), the base-material that will ferment is transported to another group of top by one group of lower floor
Layer, carries out cycle operation.
Third, sterilization system:1. installed in the uviol lamp 8 and the ozone generator 15 of casing 20 of casing 20, it is used for
The disinfection of space and equipment in case;2. microwave sterilization conveyer 2 is used for the sterilizing of base-material;3. air inlet duct mouthful is pacified
Air cleaner 10 is filled, the sterilization for supply is degerming.
Fourth, temperature humidity and air handling system (temperature humidity senses control module 27):Temperature control:Electric heater
12 open in fermentation initial period, are allowed to be rapidly achieved design temperature;Then by the heat supply gas of ventilation input pipe 13 of bottom or confession
Cold air, with reference to the switch of top vent window, reaches temperature constant.The spraying nozzle that humidity is installed by case top and the hydraulic pamp being connected
(or elevated tank) is adjusted.Air is also by the ventilation duct regulation supply (filtration, purification) of bottom.Humiture is controlled by single-chip microcomputer 28,
Single-chip microcomputer 28 is connected with the temperature sensor 29 and humidity sensor 19 in casing 20, realizes constant control.
Fifth, material transmission and system of stirring:The entrance of fermentation materials is completed with output by conveyer belt.Fermentation bed material
Stir by 30 timing rotation of fermentation crawler belt layer (starting switch during to conveying motor power setting) or continuously run at a slow speed, by first
Fermentation bed 22 (feeding mouth rises) upper materials successively pass to lower floor, and second is transported to through high inclination-angle conveyer belt (first conveyer belt 23)
The top layer of fermentation bed 24;The material of second fermentation bed 24 is same to phoresy bottom by top layer, then by (the second conveying of high inclination-angle conveyer belt
Band 26) lifting, to first group of top layer, so stirs again and again;Installed about at the top of every layer of fermentation crawler belt layer 30 simultaneously
Rake device is turned in dry lifting, can realize stirring for material while fermentation bed is operated according to being lifted the need for fermentation
Or do not stir.The material finally fermented is spread out of at the bottom end of second group of conveyer belt bed through the window that discharges by conveyer belt.
The solid fermentation apparatus that the present invention is designed, according to fermented bacterium, particularly edible fungal hypha it is aerobic it is sufficient without
The characteristics of preferably stirring more, takes " the circulating fermenting case of biserial layer frame endless track (fermentation bed mechanism) " (principle can also set accordingly
It is calculated as multiple row), temperature automatically controlled damping;Indoor sterilization sterilization and external microwave or steam high-temperature material that ultraviolet, ozone is combined
Disinfection, makes miscellaneous bacteria breeding be reduced to bottom line, ensures the abundant breeding and fermentation of beneficial bacterium;Hot blast/cold wind is equipped with to supply
To device, and it is interior set sprayer unit, automatic ventilation, temperature control, damping ensure the environment of solid state fermentation;Crawler belt induction system makes pre-
The base-material of processing is automatically entered, and is mixed by mixer with strain, and pedrail self-regulating timing rotation or low speed are continuously rotated, and realization is turned over
Material and transmission, can automatically spread out of after fermentation, can continuously ferment, also can Batch fermentation.Present apparatus temperature, wet, gas auto-control;Production
Energy consumption is low, and nutriment loss is few;High-volume or consecutive production;Fermentation is abundant, and functional mass content is high, and quality is significantly carried
Height, good combination property.
The present invention also provides a kind of method that waste matter of fermenting produces high activity high nutrition feed, using above-mentioned fermentation waste matter
The equipment for producing high activity high nutrition feed, comprises the following steps:
(1) strain and its preparation
1st, the selection of strain:The strain that the present invention is screened is four classes:Eat (medicine) (mould with fungi, fodder yeast, filamentous fungi
Bacterium), prebiotic Bacillus, be aerobic bacteria, middle pyschrophile.The standard of screening is the non-toxic suspicion of tool, and growth and breeding is fast, adaptability
By force, generation active material is more, and concertedness is good between strain, the features such as biological function is with strong complementarity.
(1) edible mushroom:Grow fast, albumen and the high mesophile kind of essential amino acids content, including flat mushroom, Pleurotus nebrodensis, tea
Set mushroom, pleurotus eryngii, delicious lactarius, mushroom, Hericium erinaceus, straw mushroom, phoenix-tail mushroom etc.;
(2) saccharomycete:Breeding is fast, high conversion rate, mycoprotein and vitamin equal size are high, including saccharomyces cerevisiae, the Chinese are inferior
Saccharomycete, Pichia pastoris, cloth Laplace saccharomycete, candidiasis, Endomycopsis Fibnligera, geotrichum candidum etc.;
(3) filamentous fungi (mould):Zymogenic rate is high, and the lower growth of middle temperature is fast, including aspergillus niger, aspergillus oryzae, monascus, wood
Mould, head mold, mould etc.;
(4) gemma probiotics:Zymogenic rate is high, Anti-bacterium power is strong, the lower growth and breeding of middle temperature is fast etc., including bacillus subtilis
Bacterium, bacillus licheniformis, bacillus coagulans, bafillus natto etc.;
Above strain screens excellent bacterial strain and combinations thereof by single and combination culture experiment.
2nd, prepared by strain:
(1) preparation of liquid spawn
It is prepared by A, edible fungus species:The comprehensive potato juice culture medium of hypha of edible fungus inoculation is waited by what is activated by flat board
In (as PDA culture medium signified in routine techniques), its condition of culture is:15 DEG C -28 DEG C of cultivation temperature, shaking speed 60-
180r/min, incubation time 48-96h;
It is prepared by B, filamentous fungi (mould) strain:The filamentous fungis such as aspergillus, head mold, trichoderma, mould are inoculated with Czapek's medium
In, its condition of culture is:20 DEG C -28 DEG C of cultivation temperature, shaking speed 100-180r/min, incubation time 24-48h;
It is prepared by C, yeast seeds:By saccharomycete such as the candida utili, saccharomyces cerevisiae, the geotrichum candidums that are activated by flat board
It is inoculated with malt extract medium, its condition of culture is:20 DEG C -32 DEG C of cultivation temperature, shaking speed 120-180r/min, during culture
Between 24-36h;
It is prepared by D, Bacillus:By in the Bacillus inoculation nutrient broth medium activated by flat board, it is trained
Foster condition is:25 DEG C -37 DEG C of cultivation temperature, shaking speed 150-180r/min, incubation time 12-24h;
(2) preparation of solid spawn
It is prepared by A, edible fungus species:By the edible bacterium solution such as the flat mushroom prepared, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius
Body seed accesses solid medium by 5%-20% inoculum concentrations, and (solid medium is:30% corn flour, 40% wheat bran, 20% meter
Chaff, 10% dregs of beans, 0.1%MgSO4,0.1%KH2PO4) in, 15 DEG C -28 DEG C, incubation time 48-96h, mycelial density reaches ++++
(mycelia is covered with).
It is prepared by B, mold species:The filamentous fungi such as aspergillus, head mold, trichoderma, mould liquid seeds press 2%-10% inoculum concentrations
Access in solid medium (culture medium is with edible mushroom seed culture medium), 20 DEG C of -28 DEG C of culture 24-48h, mycelial density reaches ++++
(mycelia is covered with).
It is prepared by C, yeast seeds:By ferment such as the candida utili prepared, button capsule yeast, saccharomyces cerevisiae, geotrichum candidums
Female bacteria liquid seed is linked into solid medium (culture medium is with edible mushroom seed culture medium) by 2%-10%, 20 DEG C -32 DEG C
12-24h is cultivated, inoculum density is up to 1 × 1010More than cfu/g.
It is prepared by D, Bacillus:By the bacillus subtilis prepared, bacillus licheniformis, bafillus natto etc.
Bacillus liquid seeds are linked into solid fermentation culture medium by 1%-5%, and (solid medium is:40% wheat bran, 35% dregs of beans,
10% rice bran, 12.5% corn flour, 1% glucose, 0.5% ammonium sulfate, 0.1%MgSO4, 0.3%K2HPO4, 0.5%NaCl,
0.01%FeSO4·H2O in), 25 DEG C -37 DEG C, 12-24h is cultivated, strain density is up to 1 × 1011More than cfu/g.
3rd, (or combination) strain is combined to prepare:Cultured solid spawn (or liquid spawn) presses edible mushroom or mould:Ferment
Female bacterium:Bacillus=3-10:1-3:0.5-2 mass ratio is configured to composite bacteria.Also can be to the strain carry out group for preparing
Close, i.e., be inoculated with successively according to growth speed, grow first connecing for slow edible mushroom, slower filamentous fungi second connects, growth is fast
Yeast and probiotics be most followed by, time interval 12-24h, to ensure the growth times of various bacterium as degree.
(2) ferment:
1st, the preparation of fermentation base-material:
(1) poor slag compost:One kind of the poor slag such as new fresh bean dreg, schlempe, residue of soya, acid-sludge, sweet potato waste, pomace, Chinese medicine slag
Or a variety of (depending on resource situations), the one or more of the siccatives such as appropriate grass meal, rice bran, maize cob meal, wheat bran are added,
Regulate and control moisture 75-45%.Single variety grain slag, which is prepared, should be noted that:The high poor slag of carbohydrate content (potato slag etc.) adds 0.5-
2% ammonium sulfate etc. is inorganic nitrogen-sourced, or with grouts mixed preparing;Wood fibre cellulose content high poor slag (Chinese medicine slag, pomace, cassava
Slag etc.) 5-30% corn flour, wheat bran etc. are added, to ensure the starter nutrient of microorganism growth.
(2) grouts compost:In the grouts such as cotton dregs, rapeseed meal, dregs of beans, Extracted From Oil-tea-cake, peanut meal, sunflower seed dregs, palm kernel meal
One or more (depending on raw materials and product objective), after crushing, add the starch materials such as 3-20% corn flour, wheat bran,
Well mixed, add water the appropriate water content (70-40%) of modulation.
2nd, fermenting procedure
(1) sterilization:Raw material (poor slag compost or grouts compost) to be fermented, directly application (especially fresh nothing
The raw material of pollution) or sterilized sterilizing (saturated vapor high temperature or microwave disinfection).Fermenting case (room) is before charging fermentation, with ultraviolet
Line and ozone carry out sealing sterilization more than 2h.
(2) inoculation and fermentation:Fermentation base-material to be fermented once admixes 5%-20% complex solids strain (or liquid bacteria
Kind), stir, fill tray, expect thickness 5-15cm, or strain (the slow strain of growth is admixed according to growth speed by several times
First admix, grow it is slower it is placed in the middle admixes, fast last of growth is admixed, time interval using ensure the fermentation times of all kinds of strains as
Degree), the aerobic fementation under the conditions of 20-35 DEG C of temperature, relative humidity 65-100%, 1-12h is stirred once, or continuous transmission at a slow speed
Conveyer belt stirring, ferment 36-96h, treats that it is degree that white thalline is covered with compost.
(3) dry with crushing
Using 50-85 DEG C of instantaneous quick drying apparatus, (pneumatic conveying dryer or roller drying are set the waste matter feed fermented
It is standby etc.).Dried fermentation waste matter carries out being crushed to 20-60 mesh, packs, i.e. feedstuff product, is produced for mixed feed.
Product can be divided into feature energy feed and functional protein feed:General grain slag fermentation protein content is work(below 25%
Can property energy feed;Grouts ferment crude protein content is functional protein feed more than 25%.
Embodiment 1
A kind of method that waste matter of fermenting produces high activity high nutrition feed, comprises the following steps:
Step 1, prepare strain:
(1) preparation of liquid spawn:It is prepared by A, edible fungus species:The hypha of Pleurotus ostreatus activated by flat board is inoculated with and integrated
In potato juice culture medium, its condition of culture is:22 DEG C -28 DEG C of cultivation temperature, shaking speed 150-180r/min, incubation time
48h;It is prepared by B, mold species:In the aspergillus oryzae inoculation Czapek's medium activated through flat board, its condition of culture is:Cultivation temperature
20 DEG C -28 DEG C, shaking speed 150-180r/min, incubation time 48h;It is prepared by C, yeast seeds:By what is activated by flat board
In Candida inoculation malt extract medium, its condition of culture is:25 DEG C -30 DEG C of cultivation temperature, shaking speed 150-180r/
Min, incubation time 24h;It is prepared by D, Bacillus:The Bacillus activated by flat board is inoculated with nutrient broth medium
In, its condition of culture is:30 DEG C -37 DEG C of cultivation temperature, shaking speed 150-180r/min, incubation time 24h;
(2) preparation of solid spawn:It is prepared by A, edible fungus species:By the flat mushroom edible fungus liquid seed prepared by 5%-
(solid medium is 20% inoculum concentration access solid medium:30% corn flour, 40% wheat bran, 20% rice bran, 10% dregs of beans,
0.1%MgSO4, 0.1%KH2PO4) in, 22 DEG C -28 DEG C, quiescent culture time 48-72h, mycelial density reaches ++++(mycelia length
It is full).It is prepared by B, mold species:The aspergillus oryzae liquid seeds prepared access solid medium (culture by 2%-10% inoculum concentrations
Base is with edible mushroom seed culture medium) in, 20 DEG C of -28 DEG C of culture 24-48h, mycelial density reaches ++++(mycelia is covered with);C, saccharomycete
It is prepared by strain:The maternity leave prepared silk saccharomycete liquid seeds are linked into solid medium (the same food of culture medium by 2%-10%
Use bacterium seed culture medium) in, 25 DEG C of -32 DEG C of culture 24h, inoculum density is up to 1 × 1010More than cfu/g;D, Bacillus system
It is standby:By 1%-5% the bacillus subtilis liquid seeds prepared are linked into solid fermentation culture medium, and (solid medium is:
50% wheat bran, 37.5% dregs of beans, 12.5% corn flour, 1% glucose, 1% ammonium sulfate) in, 28 DEG C -37 DEG C, cultivate 24h, bacterium
Density is up to 1 × 1011More than cfu/g.
(3) composite bacteria is prepared:Cultured solid spawn presses edible fungus (or filamentous fungi):Saccharomycete:Gemma bar
Bacterium=6:1.5:0.5 mass ratio is configured to composite bacteria, or constitutes combination strain in proportion.
Step 2, take the poor slag of new fresh bean dreg, schlempe, residue of soya, acid-sludge, sweet potato waste, pomace, add appropriate grass meal, rice bran,
Maize cob meal, wheat bran siccative, regulate and control moisture 75-50%, poor slag compost are made.By the fermentation base-material prepared directly application or
The sterilized sterilizing of person;The equipment of waste matter of fermenting production high activity high nutrition feed is entered before charging fermentation with ultraviolet and ozone
Row sealing sterilization more than 2h;Fermentation base-material to be fermented once admixes 5-15% complex solid strains, or admixes combination strain by several times
Kind, stir, fill tray, expect thickness 5-15cm, the aerobic fementation under the conditions of 22-30 DEG C of temperature, relative humidity 75-100%,
1-12h is stirred once, or continuous driving and conveying belt stirring at a slow speed, and ferment 48-72h, treats that it is degree that white thalline is covered with compost.
Step 3, by the waste matter feed fermented use 50-85 DEG C of expansion drying or roller drying;Dried fermentation grain
The dregs of rice carry out being crushed to 20-60 mesh, pack, i.e. feedstuff product, are produced for mixed feed;Poor slag fermentation protein content
Less than 25%, it is feature energy feed.
Embodiment 2
A kind of method that waste matter of fermenting produces high activity high nutrition feed, comprises the following steps:
Step 1, prepare strain:
(1) preparation of liquid spawn:It is prepared by A, edible fungus species:The pleurotus nebrodensis edible mushroom mycelia that will be activated by flat board
It is inoculated with potato juice culture medium, its condition of culture is:22 DEG C -28 DEG C of cultivation temperature, shaking speed 150-180r/min, culture
Time 48h;It is prepared by B, mold species:In the black-koji mould inoculation Czapek's medium activated through flat board, its condition of culture is:Culture
20 DEG C -28 DEG C of temperature, shaking speed 150-180r/min, incubation time 48h;It is prepared by C, yeast seeds:It will be lived by flat board
In the geotrichum candidum inoculation malt extract medium of change, its condition of culture is:25 DEG C -32 DEG C of cultivation temperature, shaking speed 150-180r/
Min, incubation time 24h;It is prepared by D, Bacillus:By the inoculation nutrient broth such as natto Bacillus activated by flat board
In culture medium, its condition of culture is:28 DEG C -37 DEG C of cultivation temperature, shaking speed 150-180r/min, incubation time 24h;
(2) preparation of solid spawn:It is prepared by A, edible fungus species:By the pleurotus nebrodensis edible mushroom liquid seeds prepared by
(solid medium is 5%-20% inoculum concentrations access solid medium:30% corn flour, 40% wheat bran, 20% rice bran, 10% beans
The dregs of rice, 0.1%MgSO4, 0.1%KH2PO4) in, 26 DEG C -28 DEG C, quiescent culture time 48h, mycelial density reaches ++++(mycelia length
It is full).It is prepared by B, mold species:The aspergillus niger liquid seeds prepared access solid medium (culture by 2%-10% inoculum concentrations
Base is with edible mushroom seed culture medium) in, 20 DEG C of -28 DEG C of culture 24-48h, mycelial density reaches ++++(mycelia is covered with);C, saccharomycete
It is prepared by strain:The geotrichum candidum liquid seeds prepared are linked into solid medium (the same edible bacterium of culture medium by 2%-10%
Sub- culture medium) in, 25 DEG C of -32 DEG C of culture 24h, inoculum density is up to 1 × 1010More than cfu/g.It is prepared by C, Bacillus:Will
The Bacillus nattoSawamura liquid seeds prepared are linked into solid fermentation culture medium by 1%-5%, and (solid medium is:50% bran
Skin, 37.5% dregs of beans, 12.5% corn flour, 1% glucose, 1% ammonium sulfate) in, 28 DEG C -37 DEG C, 24h is cultivated, strain density is up to 1
×1011More than cfu/g.
(3) composite bacteria is prepared:Cultured solid spawn presses edible mushroom (or filamentous fungi):Saccharomycete:Bacillus
=10:2:1 mass ratio is configured to composite bacteria, or constitutes combination strain in proportion.
Step 2, by grouts such as cotton dregs, rapeseed meal, dregs of beans, palm kernel meals, after crushing, add 3%-20% corn flour, wheat bran
Starch materials, are well mixed, and add water the appropriate water content (70%-40%) of modulation.The fermentation base-material prepared directly should
With or sterilized sterilizing;The equipment of waste matter of fermenting production high activity high nutrition feed is before charging fermentation, with ultraviolet and smelly
Oxygen carries out sealing sterilization more than 2h;Fermentation base-material to be fermented admixes 10-20% complex solids strain or combination strain, and stirring is equal
It is even, tray is filled, thickness 5-15cm is expected, the aerobic fementation under the conditions of 22-30 DEG C of temperature, relative humidity 75-100%, 12h stirs one
It is secondary, or continuous driving and conveying belt stirring at a slow speed, ferment 48-96h, treats that it is degree that white thalline is covered with compost.
Step 3, the waste matter fermented is dried and crushed, grouts ferment crude protein content is work(more than 25%
Can property protein feed.
Embodiment 3
A kind of method that waste matter of fermenting produces high activity high nutrition feed, comprises the following steps:
Step 1, prepare strain:
(1) preparation of liquid spawn:It is prepared by A, edible fungus species:The Morciiella Esculeuta Mycelia activated by flat board is inoculated with horse
In bell potato juice culture medium, its condition of culture is:22 DEG C -28 DEG C of cultivation temperature, shaking speed 150-180r/min, incubation time
48h;It is prepared by B, filamentous fungus strain:The monascus activated through flat board is inoculated with Czapek's medium, 20 DEG C -28 DEG C, shaking table turns
Fast 150-180r/min, incubation time, 36h;It is prepared by C, yeast seeds:By the cloth Laplace saccharomycete inoculation activated by flat board
In malt extract medium, its condition of culture is:25 DEG C -32 DEG C of cultivation temperature, shaking speed 150-180r/min, incubation time
24h;It is prepared by D, Bacillus:By in the Bacillus inoculation nutrient broth medium activated by flat board, it cultivates bar
Part is:30 DEG C -37 DEG C of cultivation temperature, shaking speed 150-180r/min, incubation time 24h;
(2) preparation of solid spawn:It is prepared by A, edible fungus species:By the hickory chick liquid seeds prepared by 5%-20%
(solid medium is inoculum concentration access solid medium:30% corn flour, 40% wheat bran, 20% rice bran, 10% dregs of beans, 0.1%
MgSO4, 0.1%KH2PO4) in, 26 DEG C -28 DEG C, quiescent culture time 48h, mycelial density reaches ++++(mycelia is covered with).It is B, thread
It is prepared by fungi strain:The monascus liquid seeds prepared are inoculated into solid medium by 5-10% (to train with edible mushroom seed
Support base) in, 25 DEG C -28 DEG C, 48h is cultivated, mycelial density reaches ++++(mycelia is covered with).It is prepared by C, yeast seeds:It will prepare
Cloth Laplace yeast liquid body seed be linked into by 2%-10% in solid medium (with edible mushroom seed culture medium), 26 DEG C-
28 DEG C of culture 24h, inoculum density is up to 1 × 1010More than cfu/g.It is prepared by D, Bacillus:By the lichens brood cell's bar prepared
Bacteria liquid seed is linked into solid fermentation culture medium by 1%-5%, and (solid medium is:50% wheat bran, 37.5% dregs of beans,
12.5% corn flour, 1% glucose, 1% ammonium sulfate) in, 28 DEG C -35 DEG C, 24h is cultivated, strain density is up to 1 × 1011Cfu/g with
On.
(3) combination strain is prepared:Cultured solid (or liquid) strain presses edible mushroom:Filamentous fungi:Saccharomycete:Gemma
Bacillus=10:2:1.5::1 mass ratio is with composition combination strain.
Step 2, a kind of health care Chinese medicine (ginseng, the Radix Astragali, the fruit of Chinese wolfberry, the fruit of glossy privet, Semen Cuscutae, the fruit of Cherokee rose, barrenwort, the root of herbaceous peony,
Malt, sealwort etc.) dregs of a decoction add 15-20% edible fungus solid culture base-materials, and regulate and control moisture 50%-75%, be configured to Chinese medicine slag
Compost.By the sterilized sterilizing of the Chinese medicine slag compost prepared, 10% edible fungus species are admixed, are stirred, fill tray, material
Thickness 5-15cm, the aerobic fementation under the conditions of 28-32 DEG C of temperature, relative humidity 75-100%, 1-12h is stirred once, or at a slow speed
After continuous driving and conveying belt stirring, fermentation 24-48h, admix after 2% red yeast rice strain, fermentation 12-24h, inoculate saccharomycete and
Bacillus, ferment 12-24h, treats that it is degree that red thalline is covered with compost.
Step 3, by the Chinese medicine dreg fodder fermented use 50-85 DEG C of expansion drying or fluidized bed drying;Dried hair
The ferment dregs of a decoction carry out being crushed to 20-60 mesh, pack, as functional nutrient feed, though protein content is below 20%, crude fibre is near
20%, but rich in probiotics, amino acid, vitamin, active polysaccharide and polypeptide and health medicine composition, heath-function battalion can be made
Feed addictive is supported, is produced for mixed feed.
Illustrate the beneficial effect of this preparation method with reference to specific experimental data:
1st, fermented bacterium and combination research:
(1) food (medicine) with bacterium (flat mushroom, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius etc.) with fodder yeast (Candida,
Brewer's yeast, geotrichum candidum) concertedness experiment.
The fodder yeast of table 1 and syntrophism situation of the edible mushroom in PDA culture medium
Note:According to bacterium colony and microscopy:+ expression can grow, ++, +++ represent that growth is preferably and fine, ++++represent that growth is special
It is not good
As shown in Table 1, food (medicine) grows no inhibitory action with bacterium to fodder yeast, and Three feed saccharomycete grows good
It is good, illustrate that edible fungus and fodder yeast concertedness are preferable.
(2) concertedness of edible mushroom (flat mushroom, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius etc.) and bacillus is tested.
The edible mushroom of table 2 and syntrophism situation of the bacillus in PDA culture medium
Note:According to bacterium colony and microscopy:+ expression can grow, ++, +++ represent that growth is preferably and fine, ++++represent that growth is special
It is not good
The micromycete of table 3 and bacillus syntrophism situation in PDA culture medium
Note:According to bacterium colony and microscopy:+ expression can grow, ++, +++ represent that growth is preferably and fine, ++++represent that growth is special
It is not good
2nd, poor residue fermented feed experiment is mixed
(1) ferment base-material
Protein 15 % low nutrition formula 1 and egg is prepared by different proportion with brewex's grains, white wine slag, bean dregs, residue of soya, acid-sludge
White 20% hyperalimentation formula 2, for fermentation test.
(2) nutrient component determining of single culture and the poor slag of mixing of composite bacteria 2 kinds of trophic level of fermentation:
The nutrient component determining result of the poor slag of mixing of table 4 different strain combined fermentation, 2 kinds of trophic level
From upper table 3 and table 4, using flat mushroom, phoenix-tail mushroom and delicious lactarius as main bacteria seed, with geotrichum candidum, Candida and
Bacillus is auxiliary bacteria, the poor slag of two mixing of high and low trophic level is fermented, to the feed nutrition quality of poor slag
It is greatly improved, protein improves 34.35-50% and 10.5-32.39%, crude fibre reduction 2.3-20.25% and 3.4-
20.0%, gross energy is slightly reduced, rate of descent 0.3-1.8% and 1.5-2.7% (caused by mainly fat declines).It is aobvious from result
Show:Edible mushroom plays a major role to coarse-fibred degraded, and the degradation of particularly flat mushroom is most strong, next to that phoenix-tail mushroom, pine breast
Mushroom is worst;The raising of geotrichum candidum, Candida and bacillus to crude protein plays a major role.Resultant effect is with flat mushroom and in vain
Preferably, next to that phoenix-tail mushroom is combined, delicious lactarius combination is worst for mould, Candida, brood cell bacterium combined effect.
(3) the poor slag active matter that ferments is determined
Method:Fermented feed sample adds after 10 times of water mill slurries, ultrasonication 10min, in cold soaking mistake in 1-4 DEG C of refrigerator
At night, filtrate is filtered to take, determine bioactive substance:Protease Forint phenol method, determination of amylase dinitrosalicylic acid system is fine
The plain enzyme filter paper method of dimension;Probiotics measure dilution plate culture colony counting method;Polysaccharide determines with polypeptide and uses phenol-sulphur respectively
Acid system and determined by ultraviolet spectrophotometry, the results are shown in Table 5.
The activity substance content of table 5 is detected
Note:★ is enzymatic determination transparent circle
(4) the poor slag antimicrbial power that ferments is determined
Method:The water-soluble substances of fermented feed is carried by upper method Dip, it is determined with Odontothrips loti to pathogen (large intestine bar
Bacterium, salmonella, staphylococcus etc.) inhibitory action (inhibition zone).
Inhibitory action (unit of the fermentation waste matter feed extract of table 6 to pathogen:cm)
Fermented bacterium is combined |
Escherichia coli |
Salmonella |
Staphylococcus |
Flat mushroom+geotrichum candidum+fodder yeast+bacillus |
0.6 |
0.8 |
1.1 |
Phoenix-tail mushroom+geotrichum candidum+fodder yeast+bacillus |
0.5 |
0.9 |
1.2 |
Delicious lactarius+geotrichum candidum+fodder yeast+bacillus |
0.9 |
1.0 |
0.9 |
2nd, grouts fermentation test
Method:After leached tea oil slag, rapeseed dregs, cotton dregs are crushed, 10% wheat bran and 10% corn flour are added respectively, is added water to aqueous
Amount is moderate, 15 pounds of sterilizing 15min, and the compound bacteria (geotrichum candidum of the edible fungus of strain -70%+30% is combined by 10% inoculation:Production member
Candida:Bacillus subtilis is 2:2:1), put in tray, 25-28 DEG C, ferment 2-4d, by standard method determine crude protein,
Amino acid, ANFs (gossypol, sulphur glycosides, Tea Saponin), probiotics and Anti-bacterium activity.Experimental result is as follows:
(1) cotton dregs fermenting experiment
Gossypol content is determined using national standard GBl3086-9l methods, and protein determination is surveyed with Kjeldahl's method, and experimental result is shown in
Table 7;Probiotics assay dilution plate culture colony counting method, antibacterial power measure Odontothrips loti the results are shown in Table 8.
The cotton dregs different strain combined fermentation gossypol of table 7 and crude protein content change
Note:/ it is not survey.
Delicious lactarius and toadstool ferment effect are represented from upper table experimental result preferably, are especially fermented with probiotic combination,
Gossypol content reduces by 85.8% and 76.5% respectively, and protein content improves 8.64% and 5.51%.
Prebiotic bacterial content and suppression pathogen determination of activity in the fermentation cotton dregs of table 8
From upper table 7 and table 8, cotton dregs are through eating (medicine) with after fungi and compound combined ferment, and 2 kinds of prebiotic bacterial contents reach
107More than ufc/g, especially delicious lactarius and hickory chick joint probiotics fermention effect are good;Result shows that fermentation material is to disease simultaneously
Opportunistic pathogen has good inhibition.
(2) Rapeseed Meal by Aspergillus Fermentation is tested
By cotton dregs fermentation process, rapeseed dregs delicious lactarius, Agrocybe chaxingu, hickory chick, phoenix-tail mushroom, antrodia, Linzhi, flat mushroom etc. are more
Plant fungi and make culture experiment, only delicious lactarius can well adapt to rapeseed dregs growth, and then make combined fermentation experiment, and determine phase
Index (sulphur glycosides, which is determined, uses the ultraviolet colorimetric method of thiocarbamide, protein determination Kjeldahl's method) is closed, its experiment the results are shown in Table 9.
Sulphur glycosides and crude protein content situation of change after the Rapeseed Meal by Aspergillus Fermentation of table 9
Upper table 9 shows the degradation rate of 2 kinds of strain combinations fermentation rapeseed dregs sulphur glycosides distinguish me 52.02% and 65.35%, thick egg
It is white to improve 4.93% 6.01%.
(3) tea seed cake fermentation test
Ibid method, determines culture of a variety of foods (medicine) with fungi to tea seed cake, as a result filters out 2 kinds of Agrocybe chaxingu and delicious lactarius
Fungi can well adapt to tea seed cake growth, and then make combined fermentation test and determine index of correlation (Tea Saponin measure use vanilla
Aldehyde-sulfuric acid process, protein determination Kjeldahl's method), it the results are shown in Table 10.
Tea Saponin and crude protein content situation of change after the fermentation of the tea seed cake of table 10
Upper table 10 shows 3 kinds of edible funguses and its strain combination fermentation Oil-tea-cake, degradation rate of 3 kinds of food fungi bacterias to Tea Saponin
Agrocybe chaxingu > hickory chick > delicious lactariuses, the edible degradation rate that Tea Saponin is further improved with compound probiotic combined fermentation, tea firewood
The degradation rate of mushroom and compound bacteria combined fermentation Tea Saponin is up to 65.44%;To crude protein content increase rate delicious lactarius > hickory chicks >
Agrocybe chaxingu, edible mushroom is further improved with compound probiotic combined fermentation crude protein content, and especially hickory chick is prebiotic with being combined
Bacterium combined fermentation, crude protein content individually ferments compared with hickory chick improves (62.8/26.5) more than 1 times.
(4) grouts fermentation amino acid content change
The grouts of table 11 fermentation amino acid content situation of change
Note:" pine, sheep, tea " is respectively delicious lactarius, hickory chick and agrocybe;" closing bacterium " is geotrichum candidum, the first Candida of production
Culture with 3 kinds of bacterium of bacillus subtilis is with mass ratio 2:2:1 mixed bacteria.
As seen from Table 11, after three kinds of grouts are fermented, total amino acid content increases with the raising of protein content, and edible
Bacterium+composite probiotics ferment is higher than the content of independent edible fungus fermented amino acid;Cotton dregs amino acid content at most improves 13.5%
(hickory chick+compound bacteria);The raising 1.3% (delicious lactarius+compound bacteria) of rapeseed meal at most;Tea cake increase rate is maximum to be reached
22.02% (hickory chick+compound bacteria).Compared to total amino acid, it is necessary to the lifting amplitude of amino acid (8 kinds determine 7 kinds) total content
It is bigger, about the 2 of total amino acid increase rate times.The increase rate of various essential amino acids:Cotton dregs with hickory chick, hickory chick with
Composite probiotics ferment increase rate is maximum, and methionine, leucine, isoleucine, lysine improve more than 20%;Fermentation rapeseed meal
Mainly methionine improves most, up to 16%;Hickory chick and combinations thereof fermentation tea cake is several must amino raising 17.5-44.5%.
Half required arginine content also has raising by a relatively large margin, and the maximum increase rate of three kinds of grouts is respectively up to 32.2%, 10.8% and
66.8%.As can be seen here after grouts fermentation, obvious improvement occurs for the composition of amino acid, and nutritional quality obtains big lifting.
3rd, health care Chinese medicine slag fermentation test
A kind of health care Chinese medicine dregs of a decoction add 15% edible fungus solid fermentation base-material, fermentation substrate of breeding, with hickory chick (or pine
Newborn mushroom), monascus, the compound bacteria of cloth Laplace saccharomycete and bacillus, 28-32 DEG C of fermentation;Fermentate is determined by standard method
Main physical and chemical index and influence to Immune Function In Animals;
(1) physicochemical character is determined:
Feed nutrition and functional component situation of change before and after the compound Chinese medicine slag combined fermentation of table 12
As seen from Table 11, health care Chinese medicine slag is with 2 groups of bacterium ferment crude proteins about more than 50%, crude fibre reduction by more than 45%,
Amino acid and other active components are increased substantially.
(2) influence of the fermented tcm slag to mouse immunity is determined
The fermented tcm slag of table 13 feeds influence of the mouse to immunity and determined
As seen from Table 13, two groups of fermented tcm slags feed mouse 2 weeks with 0.5% mix fodder, and big teach of its immunologic function increases
By force.
4th, fermented soybean dregs feed feeding live pig experiment
(1) the whole feeding experiment of fermented soybean dregs
Method:Diet Formula:Testing material:Corn 660kg, dregs of beans 100kg, premix 40kg, fermented soybean dregs (siccative)
200kg, CP13.62%,;DE13.15MJ/kg;To taking care of:Fermented soybean dregs are substituted with dry bean dregs.
Test pig and packet:Greatly enhance Dasanyuan hybridization (the white Daweizi pig of Yorkshire × length) piglet 20, stochastic averagina
It is divided into 2 groups;Feeding experiment material and to taking care of, feed period 123d, i.e., from piglet to delivering for sale respectively.
As a result:Fermented soybean dregs feed piglet to effect is delivered for sale significantly, and with non-fermented soybean dregs ratio, daily gain improves 11.2%,
Feed-weight ratio 14.9% is improved, feeding cost 6.2% is reduced.It is shown in Table 12.
The fermented soybean dregs feed of table 14 whole process raising experiment result
Group |
Head number |
Starting weight/kg |
End weight/kg |
Expect consumption/kg |
Daily gain/g |
Feedstuff-meat ratio |
Money meat ratio |
Test group |
10 |
198.0 |
1214 |
2795 |
826±58 |
2.75 |
7.26 |
Control group |
10 |
198.5 |
1113 |
2890 |
743±63 |
3.16 |
7.71 |
Note:1. feed cost valency:2.6 yuan/kg of corn;4.4 yuan/kg of dregs of beans;6 yuan/kg of premix;Ferment dry 2 yuan of bean dregs/
Kg, do not ferment dry 1 yuan/kg of bean dregs.
(2) experiment of later stage pig is fattened in fermented soybean dregs feeding
Method:Dasanyuan (the white Daweizi pig of Yorkshire × length) hybridized pig 16 is greatly enhanced, 2 groups is randomly divided into, uses respectively
Above-mentioned testing material and the commodity material of certain producer's similar nutrient level are fed, feed period 15d (2012/3/20-4/5).
As a result:Table 13 is shown:With fermented soybean dregs autogamy pig feed and the big pig feed ratio of certain producer, effect significantly, improves day
Increase weight 158g, and increase rate is up to 18.65%.Though feed-weight ratio reduction by 4.9%, average weight gain feed cost reduction by 15.7%.
The experiment of later stage pig is fattened in the feeding of the fermented soybean dregs feed of table 15
Group |
Head number |
Starting weight/kg |
End weight/kg |
Daily gain/g |
Feed consumption/kg |
Feed-weight ratio |
Money meat ratio |
Control |
8 |
480 |
582 |
847±91 |
292 |
2.86:1 |
9.72:1 |
Experiment |
8 |
476 |
597 |
1005±93 |
363 |
3.0:1 |
8.4:1 |
Note:Compare the big pig complete diet pellet with certain producer, 3400 yuan/ton;2800 yuan/ton of testing material.
(3) feeding experiment of fermentation residual forage different amounts
Method:Test pig and packet:20kg or so sizes greatly enhance Dasanyuan hybridized pig (the white big defensive wall of Yorkshire × length
Pig) 50, it is randomly divided into 5 groups, every group 10;
Feed and feeding:Test group design fermented soybean dregs are basic, normal, high, 4 kinds of consumptions of superelevation Diet Formula, i.e., middle amount group
For corn and dregs of beans 76% (corn 66%, dregs of beans 10%), premix 4%, ferment dry bean dregs 20%, other consumptions are by fermentation beans
Slag increases or decreases 10%, and corn-soybean meal accordingly reduces or increased and decreased 10%.Feeding time:30d(2014、10、8—2012、11、
7)。
As a result:The result of table 14 shows the middle amount group that 20% fermented soybean dregs are added in daily ration, increases weight most fast, more than certain manufacturer's product
The weightening of feed;And feed conversion rate highest, feed cost is minimum, only the 74% of commodity material.Low amounts group daily gain and commodity material
It is close, but feed consumption and feed cost are lower than commodity material by 10.3% and 29.7% respectively.A large amount group daily gain is lower than commodity material
1.2%, feed-weight ratio is approached, but feed price is low, weightening feed cost reduction by 23.5%.Superelevation amount group, daily gain is not enough other
The 50% of test group and control group, feed-weight ratio and feed cost reach highest.As can be seen here, ferment consumption of the poor slag in swine rationses
Preferably in 10%-30%, 20% is optimised quantity.
The influence that different amounts of fermented soybean dregs increase weight to piggy in the daily ration of table 16
Note:Control group is with certain factory's complete diet pellet.
5th, fermented health-care Chinese medicine slag feeding sow experiment
With 0.2%, the fermented health-care Chinese medicine slag spice of 0.5% and 1% 3 dosage feed later stage of pregnancy sow, sow
Constitution strengthens, and Recovery is accelerated, and postweaning estrus is done sth. in advance, weaned piglet increased weight, and is improved with the increase of dosage.Knot
Fruit is shown in Table 17 and table 18.
The situation that the sow postpartum body condition of table 17 recovers
The produce surviving of son of table 18 and the situation of piglet growth
The waste matter layer frame crawler type shallow tray fermentation of the present invention produces the apparatus and method for of high activity high nutrition feed, and existing
Fermented feed compare, have the advantage that:
1st, employ edible fungus and be combined composite bacteria with, filamentous fungi, saccharomycete, bacillus etc., have complementary functions
Property is strong.Edible mushroom production lignocellulosic enzyme activity is high, the lignocellulosic formation small-molecule substance fully in degraded waste matter, favourable dynamic
Thing digests, and in addition to the nutriments such as bacterium protein, the vitamin for forming a large amount of high-quality, also produce substantial amounts of polysaccharide,
Polypeptide, biology enzyme isoreactivity material, are conducive to animal health;Filamentous fungi enzyme activity is high, is conducive to polymer substance to degrade and disappear
Change and absorb, monascus etc. also produces active function material, is conducive to animal health;Candida and geotrichum candidum breeding are fast, can profit
With non-nutritious matter (nonprotein nitrogen, methane etc.) synthesising thalli protein, nutritive value is improved;Bacillus breeding is fast, and enzyme activity is high,
Toxin and ANFs in the waste matter that can degrade, and microbial population of animal intestinal tract is adjusted as probiotics, resist germ.Excellent bacterium
Combination is planted, the fermented product of high-quality is produced.
2nd, this method whole process is aerobic fermentation, and fermented bacterium breeding is fast, and biologicak efficiency (biodegradable and conversion ratio) is high, phase
To anaerobic fermentation, fermentation is rapid, and fully.
3rd, biological characteristics of this method based on fermented bacterium, with mesophilic digestion, temperature control energy resource consumption is few, is conducive to energy-conservation
Emission reduction;Meanwhile, the fermentative bioconversion energy consumption is also few, overcomes the bad work that energy in normal fermentation product significantly declines
With the quality of guarantee product.
4th, the resultant effect of present device and technology biofermentation is good, and fermented feed quality is increased substantially, toxin and
ANFs is able to sufficient Degradation and Transformation, and the polymeric carbohydrate such as cellulose and high molecular weight protein are obtained to the limit
Degraded, the nutriment such as protein and essential amino acid increases substantially;Meanwhile, fermented feed is rich in biology enzyme, activity benefit
Raw bacterium, amino acid, small peptide, edible fungi polysaccharide and polypeptide etc., are favorably improved digestibility, the premunition of animal, improve cultivation effect
Rate and benefit.
5th, fermented product preparation livestock and poultry fish adequate diet feeding effect of the present invention is especially good:It is such as (dry with 20% fermented soybean dregs
Product) to prepare adequate diet feeding three way cross piglet 123d and deliver for sale, and non-fermented soybean dregs group ratio, daily gain improves 11,2%, carries
High feed-weight ratio 14.9%, reduces feed cost 6.2%.With 20% fermented soybean dregs autogamy pig feed and the big pig feed of commodity of certain producer
Than, effect also extremely significantly, daily gain daily gain 158g is improved, increase rate is up to 18.65%.Though feed-weight ratio reduction by 4.9%, puts down
Weightening feed cost reduction by 15.7%.The poor slag of different amounts (10%-50%) feeding experiment display fermentation is in swine rationses
Consumption is preferably in 10%-30%, and 20% resultant effect is optimised quantity.Suitable Chinese medicine slag fermentation can turn into functional feed addition
Agent, with the somatotrophic function of health care.