CN103621798A - Feed prepared by using domestic garbage and preparation method of feed - Google Patents
Feed prepared by using domestic garbage and preparation method of feed Download PDFInfo
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Abstract
The invention provides feed for feeding piglets. Raw materials of the feed comprise biodegradable domestic garbage, microbial degradation bacterium, traditional Chinese medicine residue additives, vitamin E, vitamin A, vitamin C, calcium chloride, calcium hydrophosphate, salt, copper lysine and zinc methionine. The feed is used for feeding piglets; the feed is prepared by a product which is obtained by effectively degrading the domestic garbage, killing harmful infectious microbes such as escherichia coli, and drying, so that the domestic garbage is reused, thus the problems of large occupied area and serious pollution caused by the current domestic garbage treatment process; Chinese herbal medicine is added in the feed, so that the immunity of the piglets can be improved; the gastrointestinal tract micro ecological balance of the piglets can be adjusted; the healthy growth of the piglets can be ensured; the anti-stress capability of the animal and the capability of defending against attack of foreign microorganisms of the organism can be improved; the weight increasing speed and the feed conversion rate can be improved.
Description
Technical field
The present invention relates to environmental protection technical field, relate in particular to a kind of method of utilizing house refuse to prepare feed.
Background technology
Along with the raising of people's material life quality, house refuse has become the serious problems that modern society must face.Data show according to the relevent statistics, China's rubbish annual average rate of increase reaches more than 10%, become one of the most serious country of garbage-surrounded city in the world, so China is in the urgent need to house refuse is effectively processed, to control the pollution problem of house refuse to environment.
Existing generally have landfill method, burning method and composting process to the processing method of house refuse.These methods objectively exist the problem of land occupation, contaminated environment, waste resource.
How can house refuse effectively be processed, to make full use of house refuse, make it reenter life production cycle, become a very important topic.And house refuse is carried out to microbial degradation, and will degrade resulting product as fertilizer sources or feed.Therefore, people recycle after utilizing microorganism to house refuse degraded, prepare feed, more and more come into one's own, and have very large prospect.
The application is exactly that a kind of novel house refuse of utilizing proposing is on this basis prepared into feed, the method of nutrition purposes, especially for feeding animals, by utilize microorganism be house refuse degradation bacterial agent at normal temperature to the overall process of high temperature, house refuse is carried out to fermentative degradation processing, and by the mode stirring, realized the effective degraded to house refuse, and in this process, completed harmful miscellaneous bacterias such as Escherichia coli and the dry process killed, product is prepared into feed, thereby realized recycling of house refuse, and solved the large and with serious pollution problem of the existing occupation of land that life refuse processing method is brought.
The contained effect degradation bacteria of solid fungicide for house refuse degraded providing in the application can be accelerated refuses biodegradation, can effectively the degrade organic matter of difficult degradation in percolate of percolate degradation bacteria, thereby effectively accelerate the stabilisation process of rubbish, the time of degraded has been shortened greatly.
In addition, Chinese herbal medicine generally all contains the nutritional labelings such as protein, sugar, fat, starch, vitamin, mineral matter, trace element, has abundant trophism; Polysaccharide in Chinese herbal medicine, organic acid, alkaloids, volatile oil all have immune effect, can avoid Western medicine immunoprophylaxis and animal body tissue be had to the drawbacks such as cross reaction side effect, play the immunization that strengthens body; Chinese herbal medicine can play the similar effect of hormone, and can alleviate or prevent, eliminate pheromone sample agent, is considered to surpass the hormone-like effect thing of hormone; Chinese herbal medicine have improve body defence resistance and regulate relax stress effect, improve the phagocytic activity of zooblast, the formation of enhancing antibody, and can pre-anti-virus, fungi, protozoon and spirochetal infection; Chinese herbal medicine also has dual regulation and complex function, anti-feed oxide and going mouldy.
By add Chinese herbal medicine in feed, can improve the immunity of animal, adjust the micro ecology of gastrointestinal tract balance of animal, assurance animal health is grown up, and improves animal anti-stress ability and strengthens the ability that body is resisted external microbiological attack, improves its speed of weight increment and feed conversion rate.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of novel utilize house refuse and Chinese medicine composition and is prepared into the method for feed, and the feed that this is prepared is for feeding piglet, by the effective degraded to house refuse, and in this process, completed harmful miscellaneous bacterias such as Escherichia coli and the dry process killed, product is prepared into feed, thereby realized recycling of house refuse, and solved the large and with serious pollution problem of the existing occupation of land that life refuse processing method is brought, by add Chinese herbal medicine in feed, can improve the immunity of piglet, adjust the micro ecology of gastrointestinal tract balance of animal, guarantee that pig grows up healthy and sound, improve piglet anti-stress ability and strengthen the ability that body is resisted external microbiological attack, improve its speed of weight increment and feed conversion rate.
For solving the problems of the technologies described above, the invention provides for feeding the feed of pig, its raw material comprises: biodegradable house refuse, microbial degradation bacterium, Chinese herbal feed additive, vitamin E, vitamin A, vitamin C, calcium chloride, calcium monohydrogen phosphate, salt, Copper lysinate, zinc methionine.
Wherein, the weight portion of described various raw materials is respectively biodegradable house refuse 120 weight portion～130 weight portions, microbial degradation bacterium 1 weight portion～3 weight portion, Chinese herbal feed additive 10 weight portion～20 weight portions, 0.5 weight portion～1.5 weight portion vitamin E, 0.5 weight portion～1.5 weight portion vitamin A, 0.5 weight portion～1.5 weight portion vitamin C, calcium chloride 3 weight portion～5 weight portions, calcium monohydrogen phosphate 3 weight portion～5 weight portions, salt 4 weight portion～6 weight portions, Copper lysinate 0.5 weight portion～1.5 weight portion, zinc methionine 0.5 weight portion～1.5 weight portion.
Wherein, the raw material of described Chinese herbal feed additive comprises the bighead atractylodes rhizome, the stem of noble dendrobium, wilsonii, Radix Codonopsis, Semen Lablab Album, the root of Dahurain angelica, great burdock achene, selfheal, giant knotweed, moutan bark, the fruit of summer cypress, Buddha's hand, Fructus Hordei Germinatus, radish seed, the red sage root, motherwort, sealwort, the root of herbaceous peony, Radix Liriopes and the fruit of Chinese wolfberry.
Wherein, in described bulk drug mixture, the weight portion of each raw material is respectively the bighead atractylodes rhizome 50 weight portion～60 weight portions, the stem of noble dendrobium 25 weight portion～35 weight portions, wilsonii 30 weight portion～40 weight portions, Radix Codonopsis 70 weight portion～80 weight portions, Semen Lablab Album 30 weight portion～40 weight portions, the root of Dahurain angelica 20 weight portion～30 weight portions, great burdock achene 20 weight portion～30 weight portions, selfheal 10 weight portion～20 weight portions, giant knotweed 20 weight portion～30 weight portions, moutan bark 20 weight portion～30 weight portions, the fruit of summer cypress 10 weight portion～20 weight portions, Buddha's hand 10 weight portion～20 weight portions, Fructus Hordei Germinatus 20 weight portion～30 weight portions, radish seed 20 weight portion～30 weight portions, the red sage root 20 weight portion～30 weight portions, motherwort 15 weight portion～25 weight portions, sealwort 40 weight portion～50 weight portions, the root of herbaceous peony 20 weight portion～30 weight portions, Radix Liriopes 30 weight portion～40 weight portions and the fruit of Chinese wolfberry 25 weight portion～35 weight portions.
Wherein, described Chinese herbal feed additive is specifically prepared as:
Each component in described Chinese herbal feed additive is mixed in proportion, add subsequently the water with respect to 3～5 times of mixture qualities, decoct 2～4 hours, filter, obtain the first extract, filter residue adds the water with respect to 1～3 times of its quality again, decoct 1～2 hour, filter, obtain the second extract, extract is merged, reduced pressure concentration, except anhydrating, obtains dry lotion, adopts super-fine powder mill to pulverize, grinding particle size 200 order～300 orders, obtain Powdered Chinese herbal feed additive.
Wherein, described microbial degradation bacterium is by Lactobacillus plantarum agent and bacillus licheniformis agent, and described Lactobacillus plantarum agent and bacillus licheniformis agent mass ratio are 1:1.
Wherein, the preparation of described Lactobacillus plantarum agent is specially:
The first step activates Lactobacillus plantarum after 24h at 32～36 ℃, is inoculated in enrichment culture liquid, 32～36 ℃ of temperature, under rotating speed 220～250rpm condition, cultivates 24h;
Second step, then by the bacterial classification after enrichment by volume mark 2～5% be inoculated in and in aerobic fermentation nutrient solution, carry out aerobic fermentation cultivation, condition of culture is: 32～36 ℃ of temperature, rotating speed 170～200rpm, Ventilation Rate 0.8～1.0L ∕ Lmin, the air passing into is crossed 0.45 μ m filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 20h～24h, last natural air drying or 30 ℃ of oven dry, obtain solid fungicide, and the bacteria containing amount in solid fungicide is 10
10-11cfu/g, described enrichment culture liquid composition is sodium chloride 5.0～6.0g, peptone 5.0～6.0g, powdered beef 2.5～3.0g, distilled water 1000mL, pH7.0～7.2, described fermentation culture composition is glucose 45g～50g, magnesium sulfate 10g～20g, potassium dihydrogen phosphate 10g～30g, dipotassium hydrogen phosphate 10g～50g, distilled water 1000mL, regulating pH value is 7.0～7.2.
Wherein, the preparation of described bacillus licheniformis agent is specially:
The first step activates bacillus licheniformis after 24h at 20～30 ℃, is inoculated in enrichment culture liquid, 20～30 ℃ of temperature, under rotating speed 250～280rpm condition, cultivates 24h;
Second step, then by the bacterial classification after enrichment by volume mark 2～5% be inoculated in and in aerobic fermentation nutrient solution, carry out aerobic fermentation cultivation, condition of culture is: 20～30 ℃ of temperature, rotating speed 170～200rpm, Ventilation Rate 1.0～1.2L ∕ Lmin, the air passing into is crossed 0.45 μ m filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 20h～24h, last natural air drying or 30 ℃ of oven dry, obtain solid fungicide, and the bacteria containing amount in solid fungicide is 10
10-11cfu/g, described enrichment culture liquid composition is potato 180.0g～220.0g, glucose 18.0～22.0g, distilled water 1000ml, pH6.0～6.2, described fermentation culture composition is corn flour 5.0g～5.5g, beancake powder 5.0g～5.5g, peanut powder 5.0g～5.5g, KH
2pO
42.5g～3.0g, MgSO
40.25g～0.3g, CaCl
20.25g～0.3g, urea 0.24g～0.3g, brown sugar 5.0g～5.3g, pH5.5～6.0, distilled water 1000ml.
Wherein, the present invention also provides the preparation method of above-mentioned feed, and it comprises:
The first step, the sorting classification of house refuse, collects degradable house refuse;
Second step, by degradable house refuse and the mixing in proportion of microbial degradation bacterium, salt, Copper lysinate, zinc methionine, carries out biodegradation fermentation, obtains fermented feed;
The 3rd step, adds Chinese herbal feed additive, vitamin E, vitamin A, vitamin C, calcium chloride in proportion in the feed after fermentation, calcium monohydrogen phosphate, is uniformly mixed, and obtains product feed.
In described second step, sweat is segmented process, advanced person's aerobe fermentation of acting charitably, carry out again anaerobic fermentation, further, degradable house refuse and salt, Copper lysinate, zinc methionine are mixed completely in proportion, add subsequently microbial degradation bacterium, remaining on temperature is 33～35 ℃, relative humidity is aerlbic culture 12～16 hours under 60%～70% condition, in proceeding to airtight polybag, continues anaerobic fermentation 10～12 days under the condition of 40～45 ℃, obtains fermented feed.
Beneficial effect of the present invention:
Feed provided by the invention is used for feeding piglet, by the effective degraded to house refuse, and in this process, completed harmful miscellaneous bacterias such as Escherichia coli and the dry process killed, product is prepared into feed, thereby realized recycling of house refuse, and solved the large and with serious pollution problem of the existing occupation of land that life refuse processing method is brought, by add Chinese herbal medicine in feed, can improve the immunity of piglet, adjust the micro ecology of gastrointestinal tract balance of piglet, guarantee that pig grows up healthy and sound, improve animal anti-stress ability and strengthen the ability that body is resisted external microbiological attack, improve its speed of weight increment and feed conversion rate.
The specific embodiment
The invention provides for feeding the feed of pig, its raw material comprises: biodegradable house refuse, microbial degradation bacterium, Chinese herbal feed additive, vitamin E, vitamin A, vitamin C, calcium chloride, calcium monohydrogen phosphate, salt, Copper lysinate, zinc methionine.
Further, described feed only consists of above-mentioned raw materials.
The weight portion of described various raw materials is respectively biodegradable house refuse 120 weight portion～130 weight portions, microbial degradation bacterium 1 weight portion～3 weight portion, Chinese herbal feed additive 10 weight portion～20 weight portions, 0.5 weight portion～1.5 weight portion vitamin E, 0.5 weight portion～1.5 weight portion vitamin A, 0.5 weight portion～1.5 weight portion vitamin C, calcium chloride 3 weight portion～5 weight portions, calcium monohydrogen phosphate 3 weight portion～5 weight portions, salt 4 weight portion～6 weight portions, Copper lysinate 0.5 weight portion～1.5 weight portion, zinc methionine 0.5 weight portion～1.5 weight portion.
The weight portion of described various raw materials is respectively biodegradable house refuse 125 weight portions, microbial degradation bacterium 2 weight portions, Chinese herbal feed additive 15 weight portions, 1 weight portion vitamin E, 1 weight portion vitamin A, 1 weight portion vitamin C, calcium chloride 4 weight portions, calcium monohydrogen phosphate 4 weight portions, salt 5 weight portions, Copper lysinate 1 weight portion, zinc methionine 1 weight portion.
The raw material of described Chinese herbal feed additive comprises the bighead atractylodes rhizome, the stem of noble dendrobium, wilsonii, Radix Codonopsis, Semen Lablab Album, the root of Dahurain angelica, great burdock achene, selfheal, giant knotweed, moutan bark, the fruit of summer cypress, Buddha's hand, Fructus Hordei Germinatus, radish seed, the red sage root, motherwort, sealwort, the root of herbaceous peony, Radix Liriopes and the fruit of Chinese wolfberry.
Further, described Chinese herbal feed additive only consists of above-mentioned raw materials.
In described bulk drug mixture, the weight portion of each raw material is respectively the bighead atractylodes rhizome 50 weight portion～60 weight portions, the stem of noble dendrobium 25 weight portion～35 weight portions, wilsonii 30 weight portion～40 weight portions, Radix Codonopsis 70 weight portion～80 weight portions, Semen Lablab Album 30 weight portion～40 weight portions, the root of Dahurain angelica 20 weight portion～30 weight portions, great burdock achene 20 weight portion～30 weight portions, selfheal 10 weight portion～20 weight portions, giant knotweed 20 weight portion～30 weight portions, moutan bark 20 weight portion～30 weight portions, the fruit of summer cypress 10 weight portion～20 weight portions, Buddha's hand 10 weight portion～20 weight portions, Fructus Hordei Germinatus 20 weight portion～30 weight portions, radish seed 20 weight portion～30 weight portions, the red sage root 20 weight portion～30 weight portions, motherwort 15 weight portion～25 weight portions, sealwort 40 weight portion～50 weight portions, the root of herbaceous peony 20 weight portion～30 weight portions, Radix Liriopes 30 weight portion～40 weight portions and the fruit of Chinese wolfberry 25 weight portion～35 weight portions.
Further, in described bulk drug mixture, the weight portion of each raw material is respectively the bighead atractylodes rhizome 55 weight portions, the stem of noble dendrobium 30 weight portions, wilsonii 35 weight portions, Radix Codonopsis 75 weight portions, Semen Lablab Album 35 weight portions, the root of Dahurain angelica 25 weight portions, great burdock achene 25 weight portions, selfheal 15 weight portions, giant knotweed 25 weight portions, moutan bark 25 weight portions, the fruit of summer cypress 15 weight portions, Buddha's hand 15 weight portions, Fructus Hordei Germinatus 25 weight portions, radish seed 25 weight portions, the red sage root 25 weight portions, motherwort 20 weight portions, sealwort 45 weight portions, the root of herbaceous peony 25 weight portions, Radix Liriopes 35 weight portions and the fruit of Chinese wolfberry 30 weight portions.
Described Chinese herbal feed additive is specifically prepared as:
Each component in described Chinese herbal feed additive is mixed in proportion, add subsequently the water with respect to 3～5 times of mixture qualities, decoct 2～4 hours, filter, obtain the first extract, filter residue adds the water with respect to 1～3 times of its quality again, decoct 1～2 hour, filter, obtain the second extract, extract is merged, reduced pressure concentration, except anhydrating, obtains dry lotion, adopts super-fine powder mill to pulverize, grinding particle size 200 order～300 orders, obtain Powdered Chinese herbal feed additive.
The present invention also provides the preparation method of above-mentioned feed, and it comprises:
The first step, the sorting classification of house refuse, collects degradable house refuse;
Second step, by degradable house refuse and the mixing in proportion of microbial degradation bacterium, salt, Copper lysinate, zinc methionine, carries out biodegradation fermentation, obtains fermented feed;
The 3rd step, adds Chinese herbal feed additive, vitamin E, vitamin A, vitamin C, calcium chloride in proportion in the feed after fermentation, calcium monohydrogen phosphate, is uniformly mixed, and obtains product feed.
In described second step, sweat is segmented process, advanced person's aerobe fermentation of acting charitably, carry out again anaerobic fermentation, further, degradable house refuse and salt, Copper lysinate, zinc methionine are mixed completely in proportion, add subsequently microbial degradation bacterium, remaining on temperature is 33～35 ℃, relative humidity is aerlbic culture 12～16 hours under 60%～70% condition, in proceeding to airtight polybag, continues anaerobic fermentation 10～12 days under the condition of 40～45 ℃, obtains fermented feed.
Described microbial degradation bacterium is by Lactobacillus plantarum agent and bacillus licheniformis agent, and described Lactobacillus plantarum agent and bacillus licheniformis agent mass ratio are 1:1.
The preparation of described Lactobacillus plantarum agent is specially:
The first step activates Lactobacillus plantarum after 24h at 32～36 ℃, is inoculated in enrichment culture liquid, 32～36 ℃ of temperature, under rotating speed 220～250rpm condition, cultivates 24h;
Second step, then by the bacterial classification after enrichment by volume mark 2～5% be inoculated in and in aerobic fermentation nutrient solution, carry out aerobic fermentation cultivation, condition of culture is: 32～36 ℃ of temperature, rotating speed 170～200rpm, Ventilation Rate 0.8～1.0L ∕ Lmin, the air passing into is crossed 0.45 μ m filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 20h～24h, last natural air drying or 30 ℃ of oven dry, obtain solid fungicide, and the bacteria containing amount in solid fungicide is 10
10-11cfu/g, described enrichment culture liquid composition is sodium chloride 5.0～6.0g, peptone 5.0～6.0g, powdered beef 2.5～3.0g, distilled water 1000mL, pH7.0～7.2, described fermentation culture composition is glucose 45g～50g, magnesium sulfate 10g～20g, potassium dihydrogen phosphate 10g～30g, dipotassium hydrogen phosphate 10g～50g, distilled water 1000mL, regulating pH value is 7.0～7.2.
The preparation of described bacillus licheniformis agent is specially:
The first step activates bacillus licheniformis after 24h at 20～30 ℃, is inoculated in enrichment culture liquid, 20～30 ℃ of temperature, under rotating speed 250～280rpm condition, cultivates 24h;
Second step, then by the bacterial classification after enrichment by volume mark 2～5% be inoculated in and in aerobic fermentation nutrient solution, carry out aerobic fermentation cultivation, condition of culture is: 20～30 ℃ of temperature, rotating speed 170～200rpm, Ventilation Rate 1.0～1.2L ∕ Lmin, the air passing into is crossed 0.45 μ m filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 20h～24h, last natural air drying or 30 ℃ of oven dry, obtain solid fungicide, and the bacteria containing amount in solid fungicide is 10
10-11cfu/g, described enrichment culture liquid composition is potato 180.0g～220.0g, glucose 18.0～22.0g, distilled water 1000ml, pH6.0～6.2, described fermentation culture composition is corn flour 5.0g～5.5g, beancake powder 5.0g～5.5g, peanut powder 5.0g～5.5g, KH
2pO
42.5g～3.0g, MgSO
40.25g～0.3g, CaCl
20.25g～0.3g, urea 0.24g～0.3g, brown sugar 5.0g～5.3g, pH5.5～6.0, distilled water 1000ml.
Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure of reaching technique effect can fully understand and implement according to this.
The preparation of embodiment 1 Lactobacillus plantarum solid fungicide
Lactobacillus plantarum (Shanghai enzyme joins biological Consumables Co., Ltd. to be provided) is activated after 24h at 33 ℃, be inoculated in enrichment culture liquid, described enrichment culture liquid composition is sodium chloride 5.0g, peptone 5.0g, powdered beef 2.5g, distilled water 1000mL, pH7.0, 33 ℃ of temperature, under rotating speed 250rpm condition, cultivate 24h, then by the bacterial classification after enrichment by volume mark 3% be inoculated in and in aerobic fermentation nutrient solution, carry out aerobic fermentation cultivation, described fermentation culture composition is: glucose 50g, magnesium sulfate 20g, potassium dihydrogen phosphate 20g, dipotassium hydrogen phosphate 30g, distilled water 1000mL, regulating pH value is 7.0, condition of culture is: 33 ℃ of temperature, rotating speed 200rpm, Ventilation Rate 1.0L ∕ Lmin, the air passing into is crossed 0.45 μ m filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 24h, finally 30 ℃ of oven dry, obtain solid fungicide, bacteria containing amount in solid fungicide is 10
10-11cfu/g.
The preparation of embodiment 2 bacillus licheniformis solid fungicides
Bacillus licheniformis (North Sea Qun Lin biotech firm provides) is activated after 24h at 25 ℃, be inoculated in enrichment culture liquid, 25 ℃ of temperature, under rotating speed 260rpm condition, cultivate 24h, then by the bacterial classification after enrichment by volume mark 5% be inoculated in and in aerobic fermentation nutrient solution, carry out aerobic fermentation cultivation, condition of culture is: 25 ℃ of temperature, rotating speed 180rpm, Ventilation Rate 1.2L ∕ Lmin, the air passing into is crossed 0.45 μ m filter membrane and is carried out filtration sterilization, and aerobic fermentation incubation time is 24h, last natural air drying, obtain solid fungicide, the bacteria containing amount in solid fungicide is 10
10-11cfu/g, described enrichment culture liquid composition is potato 200.0g, glucose 20.0g, distilled water 1000ml, pH6.0, described fermentation culture composition is corn flour 5.2g, beancake powder 5.2g, peanut powder 5.2g, KH
2pO
42.5g, MgSO
40.25g, CaCl
20.25g, urea 0.28g, brown sugar 5.0g, pH5.5, distilled water 1000ml.
The preparation of embodiment 3 Chinese herbal feed additives
By bighead atractylodes rhizome 55g, stem of noble dendrobium 30g, wilsonii 35g, Radix Codonopsis 75g, Semen Lablab Album 35g, root of Dahurain angelica 25g, great burdock achene 25g, selfheal 15g, giant knotweed 25g, moutan bark 25g, fruit of summer cypress 15g, Buddha's hand 15g, Fructus Hordei Germinatus 25g, radish seed 25g, red sage root 25g, motherwort 20g, sealwort 45g, root of herbaceous peony 25g, Radix Liriopes 35g and fruit of Chinese wolfberry 30g, mix, the water that adds subsequently 2.4kg, decoct 4 hours, filter, obtain the first extract, filter residue adds the water with respect to 3 times of its quality again, decoct 2 hours, filter, obtain the second extract, extract is merged, reduced pressure concentration is except anhydrating, obtain dry lotion, employing super-fine powder mill is pulverized, grinding particle size 200 orders, obtain Powdered Chinese herbal feed additive.
The preparation of embodiment 4 feeds 1
By the sorting classification of house refuse, collect degradable house refuse, mud is by artificial or mechanical sorting, by the salt of degradable house refuse 12.5kg and 0.5kg, Copper lysinate 0.1kg, zinc methionine 0.1kg mixes completely, the Lactobacillus plantarum solid fungicide 0.1kg that adds subsequently embodiment 1 preparation, the bacillus licheniformis solid fungicide 0.1kg of embodiment 2 preparations, remaining on temperature is 35 ℃, relative humidity is aerlbic culture 16 hours under 70% condition, in proceeding to airtight polybag, under the condition of 45 ℃, continue anaerobic fermentation 12 days, obtain fermented feed, the Chinese herbal feed additive 1.5kg that adds embodiment 3 preparations in feed after fermentation, 0.1kg vitamin E, 0.1kg vitamin A, 0.1kg vitamin C, 0.4kg calcium chloride, 0.4kg calcium monohydrogen phosphate, be uniformly mixed, obtain product feed 1.
The preparation of comparative example 1 feed 2
By the sorting classification of house refuse, collect degradable house refuse, mud is by artificial or mechanical sorting, by the salt of degradable house refuse 12.5kg and 0.5kg, Copper lysinate 0.1kg, zinc methionine 0.1kg mixes completely, the Lactobacillus plantarum solid fungicide 0.1kg that adds subsequently embodiment 1 preparation, the bacillus licheniformis solid fungicide 0.1kg of embodiment 2 preparations, remaining on temperature is 35 ℃, relative humidity is aerlbic culture 16 hours under 70% condition, in proceeding to airtight polybag, under the condition of 45 ℃, continue anaerobic fermentation 12 days, obtain fermented feed, in feed after fermentation, add 0.1kg vitamin E, 0.1kg vitamin A, 0.1kg vitamin C, 0.4kg calcium chloride, 0.4kg calcium monohydrogen phosphate, be uniformly mixed, obtain product feed 2.
Feed efficiency
150 of 30～35 age in days DLY three way cross piglets choosing clinical examination health, are divided into 2 groups at random according to sex and body weight, and 75 every group, piglet wean on the 24th, goes to nursery house after wean, and raise in semi-enclosed pig house on single hurdle, free choice feeding, drinking-water.
Experiment feed
Experimental group adopts the feed 1 of embodiment 4 preparations to feed, and control group adopts the feed 2 of comparative example 1 preparation to feed, and adds conventional antibiotic and chemicals.
Main agents
The former installed reagents of serum immune globulin reagent Wei Deling company's import, lot number: (2010) No. 3402801; Liquaemin, RMPI-1640 nutrient solution, the 96 flat Tissue Culture Plates in hole, Hank ' s liquid, PHA, MTT, DMSO solution; Live vaccines of hog cholera (Zhu Wenkang), Fuzhou Dabeinong Bioisystech Co., Ltd produces, lot number 101324.
Zoopery design
Test arrangement carries out in Haikang, Shanghai breeding stock Co., Ltd pasture, and the time is from August, 2010～2011 year October (wherein trial test is 1 week).Experimental animal is the DLY ternary weanling pig of same nursery house, and initial body weight is close, and group difference is not remarkable.In same nursery house, be divided at random 2 groups, 75 of control groups, the basal diet of feeding (adding conventional antibiotic and chemicals), 75 of test group, the feed 1 of embodiment 4 preparations of feeding.The vaccine injection time: the 2nd day pseudo-mad dog live vaccine of injection after test, test hog cholera vaccine head after 1 week and exempt from, after 5 weeks, hog cholera vaccine two is exempted from, and two groups of vaccinate conditionally completes are consistent.
Feeding and management
Pig house, raising condition, hygienic conditions, feed quality and other feedings and managements etc. are all identical, free choice feeding and drinking-water.
Blood specimen collection
Before test, test after the stage, test after two stages the mode of three blood samplings is taked in blood specimen collection.Early morning on an empty stomach, respectively from two groups of living body blood drawings, every group random 10, venous blood collection, every pig aseptic collection 10mL blood.Be divided in 3 centrifuge tubes, wherein, after the standing 1h of 2 pipe, 3000r/min is centrifugal, gets upper serum and is sub-packed in eppendorf pipe, saves backup, and 1 pipe (adding 1% liquaemin anti-freezing) send experimental determination immediately.
Test item and method
The mensuration of serum immune globulin
The level of serum immune globulin (IgG, IgM, IgA), is all used the full-automatic specific protein analyzer (BNP) of moral spirit, adopts terminal scattering turbidimetry method to measure.
The mensuration of lymphocyte transformation rate
Adopt colorimetric method (mtt assay) to detect Function of lymphocyte transform.Mtt assay is a kind of method of newer check lymphocyte function.Get anticoagulation 3mL, the dilution of equivalent Hank ' s liquid, is added on 3mL lymphocyte separation medium upper strata, the centrifugal 10min of 2000r/min, draw buffy coat, move into aseptic centrifuge tube, appropriate Hank ' s liquid washing 2 times, the centrifugal 10min of 2000r/min, then cell is suspended in (containing 100ml/L calf serum) in RMPI-1640 complete culture solution, 6g/L trypan blue exclusion, viable count (>95%), adjusting cell concentration is 5 * 10
6individual/mL.The cell suspension of dilution is cultivated on 96 porocyte culture plates, and every hole adds 50 μ L, and control group adds 50 μ LRMPI-1640 complete culture solutions, and every hole to add concentration be 20 μ g/mLConA is established 5 repetitions, put 5%CO for every group
2, 37 ℃ of cultivations, cultivate 72h.Cultivation finishes front 3h, adds 10g/LMTT10 μ L, continues to cultivate.After cultivation finishes, the centrifugal 10min of 2000r/min under room temperature, the supernatant that inclines, adds 150 μ LDMSO solution, after purple crystal is dissolved completely, detects each hole OD value with ELIASA under 570nm wavelength.By following formula, calculate lymphocyte transformation rate: lymphocyte transformation rate (%)=(test group OD value-control group OD value)/control group OD value * 100%.
Data processing
Adopt statistics software SPASS17.0 basis statistics, data represent with mean+SD (XD), significance test of difference employing one-way analysis of variance.
Results and analysis
The impact of feed of the present invention on weanling pig serum immune globulin, the results are shown in Table 1.
Table 1 experimental group and the control group impact (unit: g/L) on weanling pig serum immune globulin
| Project | Group | 1 stage | 2 stages |
| IgG | Control group | 3.673±0.469 | 3.781±0.553 |
| ? | Chinese drug-treated group | 4.293±0.511 | 5.492±0.507 |
| IgM | Control group | 0.792±0.091 | 1.039±0.173 |
| ? | Chinese drug-treated group | 0.937±0.069 | 1.295±0.129 |
| IgA | Control group | 0.211±0.053 | 0.239±0.019 |
| ? | Chinese drug-treated group | 0.231±0.033 | 0.302±0.021 |
As can be seen from Table 1, Feed Energy of the present invention significantly improves the humoral immune function of body, strengthens immunity of organisms.
The impact of feed of the present invention on weanling pig lymphocyte transformation rate, the results are shown in Table 2.
Table 2 experimental group and the control group impact (unit: %) on weanling pig lymphocyte transformation rate
| Group | 1 stage | 2 stages |
| Control group | / | 1.37±0.31 |
| Experimental group | / | 1.63±0.34 |
Note: table intermediate value=actual value * 100
-1
Feed of the present invention can significantly improve the lymphocyte transformation rate of weanling pig, can significantly improve the specific cellular immunity function of body.
The impact of feed on piglet growth performance, the results are shown in Table 3.
The impact of table 3 piglet growth performance
As can be seen from Table 3, adopt the average end of Chinese medicine composition of the present invention to be heavily significantly higher than control group, Chinese drug-treated group average daily gain and average daily ingestion amount are also significantly higher than control group, and P<0.05, proves that this feed can significantly improve piglet growth performance.
All above-mentioned these intellectual properties of primary enforcement, do not set restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.
The above, be only preferred embodiment of the present invention, is not the present invention to be done to the restriction of other form, and any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the equivalent embodiment of equivalent variations.But every technical solution of the present invention content that do not depart from, any simple modification, equivalent variations and the remodeling above embodiment done according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (9)
1. one kind for feeding the feed of pig, it is characterized in that: the raw material of described feed comprises biodegradable house refuse, microbial degradation bacterium, Chinese herbal feed additive, vitamin E, vitamin A, vitamin C, calcium chloride, calcium monohydrogen phosphate, salt, Copper lysinate, zinc methionine.
2. feed as claimed in claim 1, it is characterized in that: the weight portion of described various raw materials is respectively biodegradable house refuse 120 weight portion～130 weight portions, microbial degradation bacterium 1 weight portion～3 weight portion, Chinese herbal feed additive 10 weight portion～20 weight portions, 0.5 weight portion～1.5 weight portion vitamin E, 0.5 weight portion～1.5 weight portion vitamin A, 0.5 weight portion～1.5 weight portion vitamin C, calcium chloride 3 weight portion～5 weight portions, calcium monohydrogen phosphate 3 weight portion～5 weight portions, salt 4 weight portion～6 weight portions, Copper lysinate 0.5 weight portion～1.5 weight portion, zinc methionine 0.5 weight portion～1.5 weight portion.
3. feed as claimed in claim 1 or 2, is characterized in that: the raw material of described Chinese herbal feed additive comprises the bighead atractylodes rhizome, the stem of noble dendrobium, wilsonii, Radix Codonopsis, Semen Lablab Album, the root of Dahurain angelica, great burdock achene, selfheal, giant knotweed, moutan bark, the fruit of summer cypress, Buddha's hand, Fructus Hordei Germinatus, radish seed, the red sage root, motherwort, sealwort, the root of herbaceous peony, Radix Liriopes and the fruit of Chinese wolfberry.
4. the feed as described in claims 1 to 3, it is characterized in that: in described bulk drug mixture, the weight portion of each raw material is respectively the bighead atractylodes rhizome 50 weight portion～60 weight portions, the stem of noble dendrobium 25 weight portion～35 weight portions, wilsonii 30 weight portion～40 weight portions, Radix Codonopsis 70 weight portion～80 weight portions, Semen Lablab Album 30 weight portion～40 weight portions, the root of Dahurain angelica 20 weight portion～30 weight portions, great burdock achene 20 weight portion～30 weight portions, selfheal 10 weight portion～20 weight portions, giant knotweed 20 weight portion～30 weight portions, moutan bark 20 weight portion～30 weight portions, the fruit of summer cypress 10 weight portion～20 weight portions, Buddha's hand 10 weight portion～20 weight portions, Fructus Hordei Germinatus 20 weight portion～30 weight portions, radish seed 20 weight portion～30 weight portions, the red sage root 20 weight portion～30 weight portions, motherwort 15 weight portion～25 weight portions, sealwort 40 weight portion～50 weight portions, the root of herbaceous peony 20 weight portion～30 weight portions, Radix Liriopes 30 weight portion～40 weight portions and the fruit of Chinese wolfberry 25 weight portion～35 weight portions.
5. the feed as described in claim 1 to 4, is characterized in that: described Chinese herbal feed additive is specifically prepared as:
Each component in described Chinese herbal feed additive is mixed in proportion, add subsequently the water with respect to 3～5 times of mixture qualities, decoct 2～4 hours, filter, obtain the first extract, filter residue adds the water with respect to 1～3 times of its quality again, decoct 1～2 hour, filter, obtain the second extract, extract is merged, reduced pressure concentration, except anhydrating, obtains dry lotion, adopts super-fine powder mill to pulverize, grinding particle size 200 order～300 orders, obtain Powdered Chinese herbal feed additive.
6. the feed as described in claim 1 to 5, is characterized in that: described microbial degradation bacterium is by Lactobacillus plantarum agent and bacillus licheniformis agent, and described Lactobacillus plantarum agent and bacillus licheniformis agent mass ratio are 1:1.
7. the feed as described in claim 1 to 6, is characterized in that: the preparation of described Lactobacillus plantarum agent is specially,
The first step activates Lactobacillus plantarum after 24h at 32～36 ℃, is inoculated in enrichment culture liquid, 32～36 ℃ of temperature, under rotating speed 220～250rpm condition, cultivates 24h;
Second step, then by the bacterial classification after enrichment by volume mark 2～5% be inoculated in and in aerobic fermentation nutrient solution, carry out aerobic fermentation cultivation, condition of culture is: 32～36 ℃ of temperature, rotating speed 170～200rpm, Ventilation Rate 0.8～1.0L ∕ Lmin, the air passing into is crossed 0.45 μ m filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 20h～24h, last natural air drying or 30 ℃ of oven dry, obtain solid fungicide, and the bacteria containing amount in solid fungicide is 10
10-11cfu/g, described enrichment culture liquid composition is sodium chloride 5.0～6.0g, peptone 5.0～6.0g, powdered beef 2.5～3.0g, distilled water 1000mL, pH7.0～7.2, described fermentation culture composition is glucose 45g～50g, magnesium sulfate 10g～20g, potassium dihydrogen phosphate 10g～30g, dipotassium hydrogen phosphate 10g～50g, distilled water 1000mL, regulating pH value is 7.0～7.2.
8. the feed as described in claim 1 to 7, is characterized in that: the preparation of described bacillus licheniformis agent is specially,
The first step activates bacillus licheniformis after 24h at 20～30 ℃, is inoculated in enrichment culture liquid, 20～30 ℃ of temperature, under rotating speed 250～280rpm condition, cultivates 24h;
Second step, then by the bacterial classification after enrichment by volume mark 2～5% be inoculated in and in aerobic fermentation nutrient solution, carry out aerobic fermentation cultivation, condition of culture is: 20～30 ℃ of temperature, rotating speed 170～200rpm, Ventilation Rate 1.0～1.2L ∕ Lmin, the air passing into is crossed 0.45 μ m filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 20h～24h, last natural air drying or 30 ℃ of oven dry, obtain solid fungicide, and the bacteria containing amount in solid fungicide is 10
10-11cfu/g, described enrichment culture liquid composition is potato 180.0g～220.0g, glucose 18.0～22.0g, distilled water 1000ml, pH6.0～6.2, described fermentation culture composition is corn flour 5.0g～5.5g, beancake powder 5.0g～5.5g, peanut powder 5.0g～5.5g, KH
2pO
42.5g～3.0g, MgSO
40.25g～0.3g, CaCl
20.25g～0.3g, urea 0.24g～0.3g, brown sugar 5.0g～5.3g, pH5.5～6.0, distilled water 1000ml.
9. the preparation method of feed described in claim 1 to 8, is characterized in that, comprising:
The first step, the sorting classification of house refuse, collects degradable house refuse;
Second step, by degradable house refuse and the mixing in proportion of microbial degradation bacterium, salt, Copper lysinate, zinc methionine, carries out biodegradation fermentation, obtains fermented feed;
The 3rd step, adds Chinese herbal feed additive, vitamin E, vitamin A, vitamin C, calcium chloride in proportion in the feed after fermentation, calcium monohydrogen phosphate, is uniformly mixed, and obtains product feed.
In described second step, sweat is segmented process, advanced person's aerobe fermentation of acting charitably, carry out again anaerobic fermentation, further, degradable house refuse and salt, Copper lysinate, zinc methionine are mixed completely in proportion, add subsequently microbial degradation bacterium, remaining on temperature is 33～35 ℃, relative humidity is aerlbic culture 12～16 hours under 60%～70% condition, in proceeding to airtight polybag, continues anaerobic fermentation 10～12 days under the condition of 40～45 ℃, obtains fermented feed.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107149045A (en) * | 2017-06-20 | 2017-09-12 | 宁国市仙之居家庭农场 | A kind of Chinese medicine pig feed including sealwort and the stem of noble dendrobium and preparation method thereof |
| CN107223765A (en) * | 2017-07-26 | 2017-10-03 | 合肥梵腾环保科技有限公司 | A kind of ecological environmental-protection cultivation feed and preparation method thereof |
| CN114436486A (en) * | 2022-01-28 | 2022-05-06 | 广西惟邦环境科技有限公司 | Preparation method for harmless disposal and resource utilization of urban building garbage |
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| CN102172389A (en) * | 2011-01-12 | 2011-09-07 | 涂传荣 | Chinese medicinal preparation having functions of promoting digestion and improving growth and development |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107149045A (en) * | 2017-06-20 | 2017-09-12 | 宁国市仙之居家庭农场 | A kind of Chinese medicine pig feed including sealwort and the stem of noble dendrobium and preparation method thereof |
| CN107223765A (en) * | 2017-07-26 | 2017-10-03 | 合肥梵腾环保科技有限公司 | A kind of ecological environmental-protection cultivation feed and preparation method thereof |
| CN114436486A (en) * | 2022-01-28 | 2022-05-06 | 广西惟邦环境科技有限公司 | Preparation method for harmless disposal and resource utilization of urban building garbage |
| CN114436486B (en) * | 2022-01-28 | 2023-10-20 | 广西惟邦环境科技有限公司 | Preparation method for harmless disposal and recycling of urban construction waste |
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| CN103621798B (en) | 2015-03-04 |
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Effective date of registration: 20170711 Address after: 221200 A District, South Ring Road, Suining County, Xuzhou, Jiangsu Patentee after: XUZHOU TIANYI ANIMAL PHARMACEUTICAL CO.,LTD. Address before: 224051 Jiangsu province Yancheng City Yingbin Road No. 9 Patentee before: YANCHENG INSTITUTE OF TECHNOLOGY |
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Address after: No. 1, Jiuxu Avenue, Suining Economic Development Zone, Xuzhou City, Jiangsu Province, 221200 Patentee after: Xuzhou Tianyi Animal Pharmaceutical Co.,Ltd. Address before: 221200 A District, South Ring Road Industrial Park, Suining County, Xuzhou, Jiangsu. Patentee before: XUZHOU TIANYI ANIMAL PHARMACEUTICAL CO.,LTD. |