CN103621798B - Feed prepared by using domestic garbage and preparation method of feed - Google Patents
Feed prepared by using domestic garbage and preparation method of feed Download PDFInfo
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Abstract
The invention provides feed for feeding piglets. Raw materials of the feed comprise biodegradable domestic garbage, microbial degradation bacterium, traditional Chinese medicine residue additives, vitamin E, vitamin A, vitamin C, calcium chloride, calcium hydrophosphate, salt, copper lysine and zinc methionine. The feed is used for feeding piglets; the feed is prepared by a product which is obtained by effectively degrading the domestic garbage, killing harmful infectious microbes such as escherichia coli, and drying, so that the domestic garbage is reused, thus the problems of large occupied area and serious pollution caused by the current domestic garbage treatment process; Chinese herbal medicine is added in the feed, so that the immunity of the piglets can be improved; the gastrointestinal tract micro ecological balance of the piglets can be adjusted; the healthy growth of the piglets can be ensured; the anti-stress capability of the animal and the capability of defending against attack of foreign microorganisms of the organism can be improved; the weight increasing speed and the feed conversion rate can be improved.
Description
Technical field
The present invention relates to environmental protection technical field, particularly relate to a kind of method utilizing house refuse to prepare feed.
Background technology
Along with the raising of people's material life quality, house refuse become modern society must faced by serious problems.Data display according to the relevent statistics, China's rubbish annual average rate of increase reaches more than 10%, become one of country that garbage-surrounded city is the most serious in the world, therefore China is in the urgent need to effectively processing house refuse, to control the pollution problem of house refuse to environment.
The existing processing method to house refuse generally has landfill method, burning method and composting process.These methods objectively also exist the problem of land occupation, contaminated environment, waste resource.
How house refuse effectively can be processed, to make full use of house refuse, make it reenter life production cycle, become a very important topic.And house refuse is carried out microbial degradation, and the product obtained degrading is as fertilizer or feed.Therefore, people utilize house refuse degraded Posterior circle for utilizing microorganism, prepare feed, more and more come into one's own, have very large prospect.
The application is exactly that a kind of novel house refuse that utilizes proposed on this basis is prepared into feed, the method of nutrition purposes, especially for feeding animals, by utilizing microorganism and house refuse degradation bacterial agent in the overall process of normal temperature to high temperature, fermentative degradation process is carried out to house refuse, and the mode by stirring, achieve the effective degraded to house refuse, and complete harmful miscellaneous bacterias such as killing Escherichia coli and dry process in this process, product is prepared into feed, thus achieve recycling of house refuse, and solve the large and with serious pollution problem of the existing occupation of land that life refuse processing method is brought.
There is provided in the application for house refuse degraded solid fungicide contained by effect degradation bacteria can accelerate refuses biodegradation, percolate degradation bacteria can effectively be degraded the organic matter of difficult degradation in percolate, thus effectively accelerate the stabilisation process of rubbish, the time of degrading is substantially reduced.
In addition, Chinese herbal medicine generally all containing nutritional labelings such as protein, sugar, fat, starch, vitamin, mineral matter, trace elements, has abundant trophism; Polysaccharide in Chinese herbal medicine, organic acid, alkaloids, volatile oil all have effect of immunity, can avoid Western medicine immunoprophylaxis and have the drawbacks such as cross reaction side effect to animal body tissue, play the immunization strengthening body; Chinese herbal medicine can play the similar effect of hormone, and can alleviate or prevent, eliminates pheromone sample agent, is considered to the hormone-like effect thing surpassing hormone; Chinese herbal medicine have improve body defenses resistance and regulate relax stress effect, improve the phagocytic activity of zooblast, the formation of enhancing antibody, and can pre-anti-virus, fungi, protozoon and spirochetal infection; Chinese herbal medicine also has dual regulation and complex function, anti-feed oxide and going mouldy.
The immunity of animal can be improved by adding Chinese herbal medicine in feed, the micro ecology of gastrointestinal tract balance of adjustment animal, guarantee animal health is grown up, and improves animal anti-stress ability and strengthens the ability that body resists external microbiological attack, improve its speed of weight increment and feed conversion rate.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of novel utilize house refuse and Chinese medicine composition and is prepared into the method for feed, and the feed this prepared is used for feeding piglet, by the effective degraded to house refuse, and complete harmful miscellaneous bacterias such as killing Escherichia coli and dry process in this process, product is prepared into feed, thus achieve recycling of house refuse, and solve the large and with serious pollution problem of the existing occupation of land that life refuse processing method is brought, the immunity of piglet can be improved by adding Chinese herbal medicine in feed, the micro ecology of gastrointestinal tract balance of adjustment animal, ensure that pig grows up healthy and sound, improve piglet anti-stress ability and strengthen the ability that body resists external microbiological attack, improve its speed of weight increment and feed conversion rate.
For solving the problems of the technologies described above, the invention provides the feed for feeding pig, its raw material comprises: biodegradable house refuse, microbial degradation bacterium, Chinese herbal feed additive, vitamin E, vitamin A, vitamin C, calcium chloride, calcium monohydrogen phosphate, salt, Copper lysinate, zinc methionine.
Wherein, the weight portion of described various raw material is respectively biodegradable house refuse 120 weight portion ~ 130 weight portion, microbial degradation bacterium 1 weight portion ~ 3 weight portion, Chinese herbal feed additive 10 weight portion ~ 20 weight portion, 0.5 weight portion ~ 1.5 part by weight of vitamin E, 0.5 weight portion ~ 1.5 part by weight of vitamin A, 0.5 weight portion ~ 1.5 part by weight of vitamin C, calcium chloride 3 weight portion ~ 5 weight portion, calcium monohydrogen phosphate 3 weight portion ~ 5 weight portion, salt 4 weight portion ~ 6 weight portion, Copper lysinate 0.5 weight portion ~ 1.5 weight portion, zinc methionine 0.5 weight portion ~ 1.5 weight portion.
Wherein, the raw material of described Chinese herbal feed additive comprises the bighead atractylodes rhizome, the stem of noble dendrobium, wilsonii, Radix Codonopsis, Semen Lablab Album, the root of Dahurain angelica, great burdock achene, selfheal, giant knotweed, moutan bark, the fruit of summer cypress, Buddha's hand, Fructus Hordei Germinatus, radish seed, the red sage root, motherwort, sealwort, the root of herbaceous peony, Radix Liriopes and the fruit of Chinese wolfberry.
Wherein, in described bulk drug mixture, the weight portion of each raw material is respectively the bighead atractylodes rhizome 50 weight portion ~ 60 weight portion, the stem of noble dendrobium 25 weight portion ~ 35 weight portion, wilsonii 30 weight portion ~ 40 weight portion, Radix Codonopsis 70 weight portion ~ 80 weight portion, Semen Lablab Album 30 weight portion ~ 40 weight portion, the root of Dahurain angelica 20 weight portion ~ 30 weight portion, great burdock achene 20 weight portion ~ 30 weight portion, selfheal 10 weight portion ~ 20 weight portion, giant knotweed 20 weight portion ~ 30 weight portion, moutan bark 20 weight portion ~ 30 weight portion, the fruit of summer cypress 10 weight portion ~ 20 weight portion, Buddha's hand 10 weight portion ~ 20 weight portion, Fructus Hordei Germinatus 20 weight portion ~ 30 weight portion, radish seed 20 weight portion ~ 30 weight portion, the red sage root 20 weight portion ~ 30 weight portion, motherwort 15 weight portion ~ 25 weight portion, sealwort 40 weight portion ~ 50 weight portion, the root of herbaceous peony 20 weight portion ~ 30 weight portion, Radix Liriopes 30 weight portion ~ 40 weight portion and the fruit of Chinese wolfberry 25 weight portion ~ 35 weight portion.
Wherein, described Chinese herbal feed additive is specifically prepared as:
Component each in described Chinese herbal feed additive is mixed in proportion, adds the water relative to mixture quality 3 ~ 5 times subsequently, decoct 2 ~ 4 hours, filter, obtain the first extract, filter residue adds the water relative to its quality 1 ~ 3 times again, decoct 1 ~ 2 hour, filter, obtain the second extract, extract is merged, reduced pressure concentration, except anhydrating, obtains dry lotion, adopts super-fine powder mill to pulverize, grinding particle size 200 order ~ 300 order, obtains Powdered Chinese herbal feed additive.
Wherein, described microbial degradation bacterium is by Lactobacillus plantarum agent and bacillus licheniformis agent, and described Lactobacillus plantarum agent and bacillus licheniformis agent mass ratio are 1:1.
Wherein, the preparation of described Lactobacillus plantarum agent is specially:
The first step, after Lactobacillus plantarum is activated 24h at 32 ~ 36 DEG C, is inoculated in enrichment culture liquid, temperature 32 ~ 36 DEG C, cultivates 24h under rotating speed 220 ~ 250rpm condition;
Second step, then by the bacterial classification after enrichment by volume mark 2 ~ 5% be inoculated in aerobic fermentation nutrient solution and carry out aerobic fermentation cultivation, condition of culture is: temperature 32 ~ 36 DEG C, rotating speed 170 ~ 200rpm, Ventilation Rate 0.8 ~ 1.0L ∕ Lmin, the air passed into is crossed 0.45 μm of filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 20h ~ 24h, last natural air drying or 30 DEG C of oven dry, obtain solid fungicide, the bacteria containing amount in solid fungicide is 10
10-11cfu/g, described enrichment culture liquid composition is sodium chloride 5.0 ~ 6.0g, peptone 5.0 ~ 6.0g, powdered beef 2.5 ~ 3.0g, distilled water 1000mL, pH7.0 ~ 7.2, described fermentation culture composition is glucose 45g ~ 50g, magnesium sulfate 10g ~ 20g, potassium dihydrogen phosphate 10g ~ 30g, dipotassium hydrogen phosphate 10g ~ 50g, distilled water 1000mL, adjust ph is 7.0 ~ 7.2.
Wherein, the preparation of described bacillus licheniformis agent is specially:
The first step, after bacillus licheniformis is activated 24h at 20 ~ 30 DEG C, is inoculated in enrichment culture liquid, temperature 20 ~ 30 DEG C, cultivates 24h under rotating speed 250 ~ 280rpm condition;
Second step, then by the bacterial classification after enrichment by volume mark 2 ~ 5% be inoculated in aerobic fermentation nutrient solution and carry out aerobic fermentation cultivation, condition of culture is: temperature 20 ~ 30 DEG C, rotating speed 170 ~ 200rpm, Ventilation Rate 1.0 ~ 1.2L ∕ Lmin, the air passed into is crossed 0.45 μm of filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 20h ~ 24h, last natural air drying or 30 DEG C of oven dry, obtain solid fungicide, the bacteria containing amount in solid fungicide is 10
10-11cfu/g, described enrichment culture liquid composition is potato 180.0g ~ 220.0g, glucose 18.0 ~ 22.0g, distilled water 1000ml, pH6.0 ~ 6.2, described fermentation culture composition is corn flour 5.0g ~ 5.5g, beancake powder 5.0g ~ 5.5g, peanut powder 5.0g ~ 5.5g, KH
2pO
42.5g ~ 3.0g, MgSO
40.25g ~ 0.3g, CaCl
20.25g ~ 0.3g, urea 0.24g ~ 0.3g, brown sugar 5.0g ~ 5.3g, pH5.5 ~ 6.0, distilled water 1000ml.
Wherein, present invention also offers the preparation method of above-mentioned feed, it comprises:
The first step, the sorting classification of house refuse, collects degradable house refuse;
Second step, by degradable house refuse and the mixing in proportion of microbial degradation bacterium, salt, Copper lysinate, zinc methionine, carries out biodegradation fermentation, obtains fermented feed;
3rd step, adds Chinese herbal feed additive, vitamin E, vitamin A, vitamin C, calcium chloride in proportion in feed after fermentation, calcium monohydrogen phosphate, is uniformly mixed, and obtains product feed.
In described second step, sweat is segmented process, advanced person acts charitably aerobe fermentation, carry out anaerobic fermentation again, further, degradable house refuse and salt, Copper lysinate, zinc methionine are mixed completely in proportion, adds microbial degradation bacterium subsequently, remaining on temperature is 33 ~ 35 DEG C, relative humidity is aerlbic culture 12 ~ 16 hours under the condition of 60% ~ 70%, continues anaerobic fermentation 10 ~ 12 days proceeding in sealed plastic bag, obtain fermented feed under the condition of 40 ~ 45 DEG C.
Beneficial effect of the present invention:
Feed provided by the invention is for feeding piglet, by the effective degraded to house refuse, and complete harmful miscellaneous bacterias such as killing Escherichia coli and dry process in this process, product is prepared into feed, thus achieve recycling of house refuse, and solve the large and with serious pollution problem of the existing occupation of land that life refuse processing method is brought, the immunity of piglet can be improved by adding Chinese herbal medicine in feed, the micro ecology of gastrointestinal tract balance of adjustment piglet, ensure that pig grows up healthy and sound, improve animal anti-stress ability and strengthen the ability that body resists external microbiological attack, improve its speed of weight increment and feed conversion rate.
Detailed description of the invention
The invention provides the feed for feeding pig, its raw material comprises: biodegradable house refuse, microbial degradation bacterium, Chinese herbal feed additive, vitamin E, vitamin A, vitamin C, calcium chloride, calcium monohydrogen phosphate, salt, Copper lysinate, zinc methionine.
Further, described feed is only made up of above-mentioned raw materials.
The weight portion of described various raw material is respectively biodegradable house refuse 120 weight portion ~ 130 weight portion, microbial degradation bacterium 1 weight portion ~ 3 weight portion, Chinese herbal feed additive 10 weight portion ~ 20 weight portion, 0.5 weight portion ~ 1.5 part by weight of vitamin E, 0.5 weight portion ~ 1.5 part by weight of vitamin A, 0.5 weight portion ~ 1.5 part by weight of vitamin C, calcium chloride 3 weight portion ~ 5 weight portion, calcium monohydrogen phosphate 3 weight portion ~ 5 weight portion, salt 4 weight portion ~ 6 weight portion, Copper lysinate 0.5 weight portion ~ 1.5 weight portion, zinc methionine 0.5 weight portion ~ 1.5 weight portion.
The weight portion of described various raw material is respectively biodegradable house refuse 125 weight portion, microbial degradation bacterium 2 weight portion, Chinese herbal feed additive 15 weight portion, 1 part by weight of vitamin E, 1 part by weight of vitamin A, 1 part by weight of vitamin C, calcium chloride 4 weight portion, calcium monohydrogen phosphate 4 weight portion, salt 5 weight portion, Copper lysinate 1 weight portion, zinc methionine 1 weight portion.
The raw material of described Chinese herbal feed additive comprises the bighead atractylodes rhizome, the stem of noble dendrobium, wilsonii, Radix Codonopsis, Semen Lablab Album, the root of Dahurain angelica, great burdock achene, selfheal, giant knotweed, moutan bark, the fruit of summer cypress, Buddha's hand, Fructus Hordei Germinatus, radish seed, the red sage root, motherwort, sealwort, the root of herbaceous peony, Radix Liriopes and the fruit of Chinese wolfberry.
Further, described Chinese herbal feed additive is only made up of above-mentioned raw materials.
In described bulk drug mixture, the weight portion of each raw material is respectively the bighead atractylodes rhizome 50 weight portion ~ 60 weight portion, the stem of noble dendrobium 25 weight portion ~ 35 weight portion, wilsonii 30 weight portion ~ 40 weight portion, Radix Codonopsis 70 weight portion ~ 80 weight portion, Semen Lablab Album 30 weight portion ~ 40 weight portion, the root of Dahurain angelica 20 weight portion ~ 30 weight portion, great burdock achene 20 weight portion ~ 30 weight portion, selfheal 10 weight portion ~ 20 weight portion, giant knotweed 20 weight portion ~ 30 weight portion, moutan bark 20 weight portion ~ 30 weight portion, the fruit of summer cypress 10 weight portion ~ 20 weight portion, Buddha's hand 10 weight portion ~ 20 weight portion, Fructus Hordei Germinatus 20 weight portion ~ 30 weight portion, radish seed 20 weight portion ~ 30 weight portion, the red sage root 20 weight portion ~ 30 weight portion, motherwort 15 weight portion ~ 25 weight portion, sealwort 40 weight portion ~ 50 weight portion, the root of herbaceous peony 20 weight portion ~ 30 weight portion, Radix Liriopes 30 weight portion ~ 40 weight portion and the fruit of Chinese wolfberry 25 weight portion ~ 35 weight portion.
Further, in described bulk drug mixture, the weight portion of each raw material is respectively the bighead atractylodes rhizome 55 weight portion, the stem of noble dendrobium 30 weight portion, wilsonii 35 weight portion, Radix Codonopsis 75 weight portion, Semen Lablab Album 35 weight portion, the root of Dahurain angelica 25 weight portion, great burdock achene 25 weight portion, selfheal 15 weight portion, giant knotweed 25 weight portion, moutan bark 25 weight portion, the fruit of summer cypress 15 weight portion, Buddha's hand 15 weight portion, Fructus Hordei Germinatus 25 weight portion, radish seed 25 weight portion, the red sage root 25 weight portion, motherwort 20 weight portion, sealwort 45 weight portion, the root of herbaceous peony 25 weight portion, Radix Liriopes 35 weight portion and the fruit of Chinese wolfberry 30 weight portion.
Described Chinese herbal feed additive is specifically prepared as:
Component each in described Chinese herbal feed additive is mixed in proportion, adds the water relative to mixture quality 3 ~ 5 times subsequently, decoct 2 ~ 4 hours, filter, obtain the first extract, filter residue adds the water relative to its quality 1 ~ 3 times again, decoct 1 ~ 2 hour, filter, obtain the second extract, extract is merged, reduced pressure concentration, except anhydrating, obtains dry lotion, adopts super-fine powder mill to pulverize, grinding particle size 200 order ~ 300 order, obtains Powdered Chinese herbal feed additive.
Present invention also offers the preparation method of above-mentioned feed, it comprises:
The first step, the sorting classification of house refuse, collects degradable house refuse;
Second step, by degradable house refuse and the mixing in proportion of microbial degradation bacterium, salt, Copper lysinate, zinc methionine, carries out biodegradation fermentation, obtains fermented feed;
3rd step, adds Chinese herbal feed additive, vitamin E, vitamin A, vitamin C, calcium chloride in proportion in feed after fermentation, calcium monohydrogen phosphate, is uniformly mixed, and obtains product feed.
In described second step, sweat is segmented process, advanced person acts charitably aerobe fermentation, carry out anaerobic fermentation again, further, degradable house refuse and salt, Copper lysinate, zinc methionine are mixed completely in proportion, adds microbial degradation bacterium subsequently, remaining on temperature is 33 ~ 35 DEG C, relative humidity is aerlbic culture 12 ~ 16 hours under the condition of 60% ~ 70%, continues anaerobic fermentation 10 ~ 12 days proceeding in sealed plastic bag, obtain fermented feed under the condition of 40 ~ 45 DEG C.
Described microbial degradation bacterium is by Lactobacillus plantarum agent and bacillus licheniformis agent, and described Lactobacillus plantarum agent and bacillus licheniformis agent mass ratio are 1:1.
The preparation of described Lactobacillus plantarum agent is specially:
The first step, after Lactobacillus plantarum is activated 24h at 32 ~ 36 DEG C, is inoculated in enrichment culture liquid, temperature 32 ~ 36 DEG C, cultivates 24h under rotating speed 220 ~ 250rpm condition;
Second step, then by the bacterial classification after enrichment by volume mark 2 ~ 5% be inoculated in aerobic fermentation nutrient solution and carry out aerobic fermentation cultivation, condition of culture is: temperature 32 ~ 36 DEG C, rotating speed 170 ~ 200rpm, Ventilation Rate 0.8 ~ 1.0L ∕ Lmin, the air passed into is crossed 0.45 μm of filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 20h ~ 24h, last natural air drying or 30 DEG C of oven dry, obtain solid fungicide, the bacteria containing amount in solid fungicide is 10
10-11cfu/g, described enrichment culture liquid composition is sodium chloride 5.0 ~ 6.0g, peptone 5.0 ~ 6.0g, powdered beef 2.5 ~ 3.0g, distilled water 1000mL, pH7.0 ~ 7.2, described fermentation culture composition is glucose 45g ~ 50g, magnesium sulfate 10g ~ 20g, potassium dihydrogen phosphate 10g ~ 30g, dipotassium hydrogen phosphate 10g ~ 50g, distilled water 1000mL, adjust ph is 7.0 ~ 7.2.
The preparation of described bacillus licheniformis agent is specially:
The first step, after bacillus licheniformis is activated 24h at 20 ~ 30 DEG C, is inoculated in enrichment culture liquid, temperature 20 ~ 30 DEG C, cultivates 24h under rotating speed 250 ~ 280rpm condition;
Second step, then by the bacterial classification after enrichment by volume mark 2 ~ 5% be inoculated in aerobic fermentation nutrient solution and carry out aerobic fermentation cultivation, condition of culture is: temperature 20 ~ 30 DEG C, rotating speed 170 ~ 200rpm, Ventilation Rate 1.0 ~ 1.2L ∕ Lmin, the air passed into is crossed 0.45 μm of filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 20h ~ 24h, last natural air drying or 30 DEG C of oven dry, obtain solid fungicide, the bacteria containing amount in solid fungicide is 10
10-11cfu/g, described enrichment culture liquid composition is potato 180.0g ~ 220.0g, glucose 18.0 ~ 22.0g, distilled water 1000ml, pH6.0 ~ 6.2, described fermentation culture composition is corn flour 5.0g ~ 5.5g, beancake powder 5.0g ~ 5.5g, peanut powder 5.0g ~ 5.5g, KH
2pO
42.5g ~ 3.0g, MgSO
40.25g ~ 0.3g, CaCl
20.25g ~ 0.3g, urea 0.24g ~ 0.3g, brown sugar 5.0g ~ 5.3g, pH5.5 ~ 6.0, distilled water 1000ml.
Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.
The preparation of embodiment 1 Lactobacillus plantarum solid fungicide
After Lactobacillus plantarum (Shanghai enzyme joins biological Consumables Co., Ltd. to be provided) is activated 24h at 33 DEG C, be inoculated in enrichment culture liquid, described enrichment culture liquid composition is sodium chloride 5.0g, peptone 5.0g, powdered beef 2.5g, distilled water 1000mL, pH7.0, temperature 33 DEG C, 24h is cultivated under rotating speed 250rpm condition, then by the bacterial classification after enrichment by volume mark 3% be inoculated in aerobic fermentation nutrient solution and carry out aerobic fermentation cultivation, described fermentation culture composition is: glucose 50g, magnesium sulfate 20g, potassium dihydrogen phosphate 20g, dipotassium hydrogen phosphate 30g, distilled water 1000mL, adjust ph is 7.0, condition of culture is: temperature 33 DEG C, rotating speed 200rpm, Ventilation Rate 1.0L ∕ Lmin, the air passed into is crossed 0.45 μm of filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 24h, last 30 DEG C of oven dry, obtain solid fungicide, bacteria containing amount in solid fungicide is 10
10-11cfu/g.
The preparation of embodiment 2 bacillus licheniformis solid fungicide
After bacillus licheniformis (North Sea Qun Lin biotech firm provides) is activated 24h at 25 DEG C, be inoculated in enrichment culture liquid, temperature 25 DEG C, 24h is cultivated under rotating speed 260rpm condition, then by the bacterial classification after enrichment by volume mark 5% be inoculated in aerobic fermentation nutrient solution and carry out aerobic fermentation cultivation, condition of culture is: temperature 25 DEG C, rotating speed 180rpm, Ventilation Rate 1.2L ∕ Lmin, the air passed into is crossed 0.45 μm of filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 24h, last natural air drying, obtain solid fungicide, bacteria containing amount in solid fungicide is 10
10-11cfu/g, described enrichment culture liquid composition is potato 200.0g, glucose 20.0g, distilled water 1000ml, pH6.0, and described fermentation culture composition is corn flour 5.2g, beancake powder 5.2g, peanut powder 5.2g, KH
2pO
42.5g, MgSO
40.25g, CaCl
20.25g, urea 0.28g, brown sugar 5.0g, pH5.5, distilled water 1000ml.
The preparation of embodiment 3 Chinese herbal feed additive
By bighead atractylodes rhizome 55g, stem of noble dendrobium 30g, wilsonii 35g, Radix Codonopsis 75g, Semen Lablab Album 35g, root of Dahurain angelica 25g, great burdock achene 25g, selfheal 15g, giant knotweed 25g, moutan bark 25g, fruit of summer cypress 15g, Buddha's hand 15g, Fructus Hordei Germinatus 25g, radish seed 25g, red sage root 25g, motherwort 20g, sealwort 45g, root of herbaceous peony 25g, Radix Liriopes 35g and fruit of Chinese wolfberry 30g, mixing, add the water of 2.4kg subsequently, decoct 4 hours, filter, obtain the first extract, filter residue adds the water relative to its quality 3 times again, decoct 2 hours, filter, obtain the second extract, extract is merged, reduced pressure concentration is except anhydrating, obtain dry lotion, employing super-fine powder mill is pulverized, grinding particle size 200 order, obtain Powdered Chinese herbal feed additive.
The preparation of embodiment 4 feed 1
The sorting of house refuse is classified, collect degradable house refuse, mud is by artificial or mechanical sorting, by the salt of degradable house refuse 12.5kg and 0.5kg, Copper lysinate 0.1kg, zinc methionine 0.1kg mixes completely, add Lactobacillus plantarum solid fungicide 0.1kg prepared by embodiment 1 subsequently, bacillus licheniformis solid fungicide 0.1kg prepared by embodiment 2, remaining on temperature is 35 DEG C, relative humidity is aerlbic culture 16 hours under the condition of 70%, anaerobic fermentation is continued 12 days proceeding in sealed plastic bag under the condition of 45 DEG C, obtain fermented feed, Chinese herbal feed additive 1.5kg prepared by embodiment 3 is added in feed after fermentation, 0.1kg vitamin E, 0.1kg vitamin A, 0.1kg vitamin C, 0.4kg calcium chloride, 0.4kg calcium monohydrogen phosphate, be uniformly mixed, obtain product feed 1.
The preparation of comparative example 1 feed 2
The sorting of house refuse is classified, collect degradable house refuse, mud is by artificial or mechanical sorting, by the salt of degradable house refuse 12.5kg and 0.5kg, Copper lysinate 0.1kg, zinc methionine 0.1kg mixes completely, add Lactobacillus plantarum solid fungicide 0.1kg prepared by embodiment 1 subsequently, bacillus licheniformis solid fungicide 0.1kg prepared by embodiment 2, remaining on temperature is 35 DEG C, relative humidity is aerlbic culture 16 hours under the condition of 70%, anaerobic fermentation is continued 12 days proceeding in sealed plastic bag under the condition of 45 DEG C, obtain fermented feed, 0.1kg vitamin E is added in feed after fermentation, 0.1kg vitamin A, 0.1kg vitamin C, 0.4kg calcium chloride, 0.4kg calcium monohydrogen phosphate, be uniformly mixed, obtain product feed 2.
Feed efficiency
Choose 30 ~ 35 age in days DLY three way cross piglet 150 of clinical examination health, be divided into 2 groups at random according to sex and body weight, often organize 75, piglet 24 days wean, goes to nursery house after wean, separated raising in semi-enclosed pig house, free choice feeding, drinking-water.
Experiment feed
The feed 1 that experimental group adopts embodiment 4 to prepare is fed, and the feed 2 that control group adopts comparative example 1 to prepare is fed, and adds Conventional antibiotic and chemicals.
Main agents
Serum immune globulin reagent is the original-pack reagent of De Ling company import, lot number: (2010) No. 3402801; Liquaemin, RMPI-1640 nutrient solution, the 96 flat Tissue Culture Plates in hole, Hank ' s liquid, PHA, MTT, DMSO solution; Live vaccines of hog cholera (Zhu Wenkang), Fuzhou Dabeinong Bioisystech Co., Ltd produces, lot number 101324.
Zoopery designs
Test arrangement carries out in Haikang, Shanghai breeding stock Co., Ltd pasture, and the time is from August, 2010 ~ in October, 2011 (wherein trial test 1 week).Experimental animal is the DLY ternary weanling pig of same nursery house, and original body mass is close, and group difference is not remarkable.2 groups are divided at random, control group 75, basal diet of feeding (adding Conventional antibiotic and chemicals), test group 75, feed 1 prepared by embodiment of feeding 4 in same nursery house.The vaccine injection time: the 2nd day pseudo-mad dog live vaccine of injection after test, test hog cholera vaccine head after 1 week and exempt from, after 5 weeks, hog cholera vaccine two is exempted from, and two groups of vaccinate conditions are completely the same.
Feeding and management
Pig house, rearing conditions, hygienic conditions, feed quality and other feedings and managements etc. are all identical, free choice feeding and drinking-water.
Blood specimen collection
Three modes of taking a blood sample are taked in blood specimen collection, before namely testing, after the stage of test, after two stages of test.Early morning on an empty stomach, respectively from two groups of living body blood drawings, often organizes random 10, venous blood collection, every pig aseptic collection 10mL blood.Be divided in 3 centrifuge tubes, after wherein 2 pipes leave standstill 1h, 3000r/min is centrifugal, gets upper serum and is sub-packed in eppendorf pipe, save backup, and 1 pipe (adding 1% liquaemin anti-freezing) send experimental determination immediately.
Test item and method
The mensuration of serum immune globulin
The level of serum immune globulin (IgG, IgM, IgA), all uses moral spirit full-automatic specific protein analyzer (BNP), adopts terminal scattered light urbidmetry to measure.
The mensuration of lymphocyte transformation rate
Colorimetric method (mtt assay) is adopted to detect Function of lymphocyte transform.Mtt assay is a kind of method of newer inspection lymphocyte function.Get anticoagulation 3mL, equivalent Hank ' s liquid dilutes, and is added on 3mL lymphocyte separation medium upper strata, the centrifugal 10min of 2000r/min, draw buffy coat, move into sterile centrifugation tube, appropriate Hank ' s liquid washs 2 times, the centrifugal 10min of 2000r/min, then cell is suspended in (containing 100ml/L calf serum) in RMPI-1640 complete culture solution, 6g/L trypan blue exclusion, viable count (>95%), adjustment cell concentration is 5 × 10
6individual/mL.The cell suspension of dilution is cultivated on 96 porocyte culture plates, and every hole adds 50 μ L, and control group adds 50 μ LRMPI-1640 complete culture solutions, every hole to add concentration be 20 μ g/mLConA, and often group establishes 5 repetitions, puts 5%CO
2, 37 DEG C cultivate, cultivate 72h.Cultivation terminates front 3h, adds 10g/LMTT10 μ L, continues to cultivate.After cultivation terminates, the centrifugal 10min of 2000r/min under room temperature, incline supernatant, adds 150 μ LDMSO solution, after purple crystal is dissolved completely, detects each hole OD value with ELIASA under 570nm wavelength.By following formulae discovery lymphocyte transformation rate: lymphocyte transformation rate (%)=(test group OD value-control group OD value)/control group OD value × 100%.
Data processing
Adopt statistics software SPASS17.0 basic statistical, data represent with mean+SD (XD), and significance test of difference adopts one-way analysis of variance.
Results and analysis
Feed of the present invention, on the impact of weanling pig serum immune globulin, the results are shown in Table 1.
Table 1 experimental group and control group are on the impact (unit: g/L) of weanling pig serum immune globulin
| Project | Group | 1 stage | 2 stages |
| IgG | Control group | 3.673±0.469 | 3.781±0.553 |
| Chinese drug-treated group | 4.293±0.511 | 5.492±0.507 | |
| IgM | Control group | 0.792±0.091 | 1.039±0.173 |
| Chinese drug-treated group | 0.937±0.069 | 1.295±0.129 | |
| IgA | Control group | 0.211±0.053 | 0.239±0.019 |
| Chinese drug-treated group | 0.231±0.033 | 0.302±0.021 |
As can be seen from Table 1, Feed Energy of the present invention significantly improves the humoral immune function of body, strengthens immunity of organisms.
Feed of the present invention, on the impact of weanling pig lymphocyte transformation rate, the results are shown in Table 2.
Table 2 experimental group and control group are on the impact (unit: %) of weanling pig lymphocyte transformation rate
| Group | 1 stage | 2 stages |
| Control group | / | 1.37±0.31 |
| Experimental group | / | 1.63±0.34 |
Note: table intermediate value=actual value × 100
-1
Feed of the present invention significantly can improve the lymphocyte transformation rate of weanling pig, can significantly improve the specific cellular immunity function of body.
Feed, on the impact of piglet growth performance, the results are shown in Table 3.
The impact of table 3 piglet growth performance
As can be seen from Table 3, the average end of Chinese medicine composition of the present invention is adopted heavily to be significantly higher than control group, Chinese drug-treated group average daily gain and average daily ingestion amount are also significantly higher than control group, P<0.05, prove that this feed can significantly improve piglet growth performance.
All above-mentioned this intellectual properties of primary enforcement, not setting restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.
The above is only preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (5)
1. one kind for feeding the feed of pig, it is characterized in that: the raw material of described feed comprises biodegradable house refuse, microbial degradation bacterium, Chinese herbal feed additive, vitamin E, vitamin A, vitamin C, calcium chloride, calcium monohydrogen phosphate, salt, Copper lysinate, zinc methionine;
The weight portion of each raw material is respectively biodegradable house refuse 120 weight portion ~ 130 weight portion, microbial degradation bacterium 1 weight portion ~ 3 weight portion, Chinese herbal feed additive 10 weight portion ~ 20 weight portion, 0.5 weight portion ~ 1.5 part by weight of vitamin E, 0.5 weight portion ~ 1.5 part by weight of vitamin A, 0.5 weight portion ~ 1.5 part by weight of vitamin C, calcium chloride 3 weight portion ~ 5 weight portion, calcium monohydrogen phosphate 3 weight portion ~ 5 weight portion, salt 4 weight portion ~ 6 weight portion, Copper lysinate 0.5 weight portion ~ 1.5 weight portion, zinc methionine 0.5 weight portion ~ 1.5 weight portion;
The raw material of described Chinese herbal feed additive is the bighead atractylodes rhizome, the stem of noble dendrobium, wilsonii, Radix Codonopsis, Semen Lablab Album, the root of Dahurain angelica, great burdock achene, selfheal, giant knotweed, moutan bark, the fruit of summer cypress, Buddha's hand, Fructus Hordei Germinatus, radish seed, the red sage root, motherwort, sealwort, the root of herbaceous peony, Radix Liriopes and the fruit of Chinese wolfberry;
The weight portion of each raw material in described Chinese herbal feed additive is respectively the bighead atractylodes rhizome 50 weight portion ~ 60 weight portion, the stem of noble dendrobium 25 weight portion ~ 35 weight portion, wilsonii 30 weight portion ~ 40 weight portion, Radix Codonopsis 70 weight portion ~ 80 weight portion, Semen Lablab Album 30 weight portion ~ 40 weight portion, the root of Dahurain angelica 20 weight portion ~ 30 weight portion, great burdock achene 20 weight portion ~ 30 weight portion, selfheal 10 weight portion ~ 20 weight portion, giant knotweed 20 weight portion ~ 30 weight portion, moutan bark 20 weight portion ~ 30 weight portion, the fruit of summer cypress 10 weight portion ~ 20 weight portion, Buddha's hand 10 weight portion ~ 20 weight portion, Fructus Hordei Germinatus 20 weight portion ~ 30 weight portion, radish seed 20 weight portion ~ 30 weight portion, the red sage root 20 weight portion ~ 30 weight portion, motherwort 15 weight portion ~ 25 weight portion, sealwort 40 weight portion ~ 50 weight portion, the root of herbaceous peony 20 weight portion ~ 30 weight portion, Radix Liriopes 30 weight portion ~ 40 weight portion and the fruit of Chinese wolfberry 25 weight portion ~ 35 weight portion,
Described microbial degradation bacterium is made up of Lactobacillus plantarum agent and bacillus licheniformis agent, and described Lactobacillus plantarum agent and bacillus licheniformis agent mass ratio are 1:1.
2. as claimed in claim 1 for feeding the feed of pig, it is characterized in that: the concrete preparation method of described Chinese herbal feed additive is:
Component each in described Chinese herbal feed additive is mixed in proportion, adds the water relative to mixture quality 3 ~ 5 times subsequently, decoct 2 ~ 4 hours, filter, obtain the first extract, filter residue adds the water relative to its quality 1 ~ 3 times again, decoct 1 ~ 2 hour, filter, obtain the second extract, extract is merged, reduced pressure concentration, except anhydrating, obtains dry lotion, adopts super-fine powder mill to pulverize, grinding particle size 200 order ~ 300 order, obtains Powdered Chinese herbal feed additive.
3. as claimed in claim 1 for feeding the feed of pig, it is characterized in that: the preparation of described Lactobacillus plantarum agent is specially,
The first step, after Lactobacillus plantarum is activated 24h at 32 ~ 36 DEG C, is inoculated in enrichment culture liquid, temperature 32 ~ 36 DEG C, cultivates 24h under rotating speed 220 ~ 250rpm condition;
Second step, by the bacterial classification after enrichment by volume mark 2 ~ 5% be inoculated in aerobic fermentation nutrient solution and carry out aerobic fermentation cultivation, condition of culture is: temperature 32 ~ 36 DEG C, rotating speed 170 ~ 200rpm, Ventilation Rate 0.8 ~ 1.0L ∕ L min, the air passed into is crossed 0.45 μm of filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 20h ~ 24h, last natural air drying or 30 DEG C of oven dry, obtain solid fungicide, the bacteria containing amount in solid fungicide is 10
10-11cfu/g, described enrichment culture liquid composition is sodium chloride 5.0 ~ 6.0g, peptone 5.0 ~ 6.0g, powdered beef 2.5 ~ 3.0g, distilled water 1000mL, pH7.0 ~ 7.2, described fermentation culture composition is glucose 45g ~ 50g, magnesium sulfate 10g ~ 20g, potassium dihydrogen phosphate 10g ~ 30g, dipotassium hydrogen phosphate 10g ~ 50g, distilled water 1000mL, adjust ph is 7.0 ~ 7.2.
4. as claimed in claim 1 for feeding the feed of pig, it is characterized in that: the preparation of described bacillus licheniformis agent is specially,
The first step, after bacillus licheniformis is activated 24h at 20 ~ 30 DEG C, is inoculated in enrichment culture liquid, temperature 20 ~ 30 DEG C, cultivates 24h under rotating speed 250 ~ 280rpm condition;
Second step, by the bacterial classification after enrichment by volume mark 2 ~ 5% be inoculated in aerobic fermentation nutrient solution and carry out aerobic fermentation cultivation, condition of culture is: temperature 20 ~ 30 DEG C, rotating speed 170 ~ 200rpm, Ventilation Rate 1.0 ~ 1.2L ∕ L min, the air passed into is crossed 0.45 μm of filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 20h ~ 24h, last natural air drying or 30 DEG C of oven dry, obtain solid fungicide, the bacteria containing amount in solid fungicide is 10
10-11cfu/g, described enrichment culture liquid composition is potato 180.0g ~ 220.0g, glucose 18.0 ~ 22.0g, distilled water 1000ml, pH6.0 ~ 6.2, described fermentation culture composition is corn flour 5.0g ~ 5.5g, beancake powder 5.0g ~ 5.5g, peanut powder 5.0g ~ 5.5g, KH
2pO
42.5g ~ 3.0g, MgSO
40.25g ~ 0.3g, CaCl
20.25g ~ 0.3g, urea 0.24g ~ 0.3g, brown sugar 5.0g ~ 5.3g, pH 5.5 ~ 6.0, distilled water 1000ml.
5., as claimed in claim 1 for feeding the preparation method of the feed of pig, it is characterized in that, comprise:
The first step, the sorting classification of house refuse, collects degradable house refuse;
Second step, mixes with microbial degradation bacterium, salt, Copper lysinate, zinc methionine in proportion by degradable house refuse, carries out biodegradation fermentation, obtains fermented feed;
3rd step, adds Chinese herbal feed additive, vitamin E, vitamin A, vitamin C, calcium chloride in proportion in feed after fermentation, calcium monohydrogen phosphate, is uniformly mixed, and obtains product feed; In described second step, sweat is segmented process, and advanced person acts charitably aerobe fermentation, then carries out anaerobic fermentation.
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| CN107223765A (en) * | 2017-07-26 | 2017-10-03 | 合肥梵腾环保科技有限公司 | A kind of ecological environmental-protection cultivation feed and preparation method thereof |
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Effective date of registration: 20170711 Address after: 221200 A District, South Ring Road, Suining County, Xuzhou, Jiangsu Patentee after: XUZHOU TIANYI ANIMAL PHARMACEUTICAL CO.,LTD. Address before: 224051 Jiangsu province Yancheng City Yingbin Road No. 9 Patentee before: YANCHENG INSTITUTE OF TECHNOLOGY |
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Address after: No. 1, Jiuxu Avenue, Suining Economic Development Zone, Xuzhou City, Jiangsu Province, 221200 Patentee after: Xuzhou Tianyi Animal Pharmaceutical Co.,Ltd. Address before: 221200 A District, South Ring Road Industrial Park, Suining County, Xuzhou, Jiangsu. Patentee before: XUZHOU TIANYI ANIMAL PHARMACEUTICAL CO.,LTD. |