CN101629150A - Compound bacteria additive for sugar beet pulp silages and preparation method and application thereof - Google Patents
Compound bacteria additive for sugar beet pulp silages and preparation method and application thereof Download PDFInfo
- Publication number
- CN101629150A CN101629150A CN200910072720A CN200910072720A CN101629150A CN 101629150 A CN101629150 A CN 101629150A CN 200910072720 A CN200910072720 A CN 200910072720A CN 200910072720 A CN200910072720 A CN 200910072720A CN 101629150 A CN101629150 A CN 101629150A
- Authority
- CN
- China
- Prior art keywords
- beet pulp
- silages
- sugar beet
- lactobacillus
- compound bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
A compound bacteria additive for sugar beet pulp silages and a preparation method and application thereof relate to a compound bacteria additive for ruminant silages and a preparation method and application thereof, solving the problem that lactobacillus is insufficient in the production of existing sugar beet pulp silages. The compound bacteria additive comprises one strain of lactococcus lactis and one strain of lactobacillus. The preparation method comprises the following steps: 1. preparation of fermentation liquor; 2. separation and purification of lactococcus lactis and lactobacillus; 3. activation of lactococcus lactis and lactobacillus; 4. preparation of microbial additive. The application is as follows: during silaging, 10-15ml of the additive is added into every kilogram of sugar beet pulp with moisture content of 65-75% through drying treatment. Through 45 days of fermentation of the sugar beet pulp treated by the additive of the invention, the silaging effect of the sugar beet pulp can be obviously improved. Comparison between a test group and a blank control group shows that in the test group, the pH value and the quantities of the mould and the yeasts are obviously reduced and the ratio of ammoniacal nitrogen to total nitrogen is obviously reduced, meanwhile, the proportion of lactic acid to total acid is obviously increased and the content of butyric acid is obviously reduced. The invention can obviously improve the silaging effect of the sugar beet pulp.
Description
Technical field
The present invention relates to ruminating animal silage composite bacterial additive and its production and application.
Background technology
Beet pulp is that the beet root process is soaked, and the residue after the squeezing sugaring is the byproduct after the beet sugar manufacture.Its output height, nutritious.Beet pulp also contains pectin, alkaloid, crude protein, crude fat, robust fibre, calcium, phosphorus and multiple amino acids except that being rich in a large amount of carbohydrate, be the high-quality feed source of ruminating animals such as ox, sheep, deer.It mainly is ensiling and granulation that at present domestic and international beet pulp utilizes mode, and the particle dregs of rice nutritive value that the back of granulating produces is very high, but costs an arm and a leg, and is mainly used in outlet.Comparatively speaking, the ensiling treatment process is simple and cost is lower, is convenient to directly promote towards the raiser.The subject matter of restriction sugar beet pulp silages has two aspects at present, and the one, water content is too high, about 90%, easily causes mildew and rot during the fermentation usually; The 2nd, lactic acid bacteria number quantity not sufficient in the beet pulp makes that beet pulp acidity descends slowly after the ensiling, and harmful microorganism such as mould and yeast restraining effect are reduced, and causes that ensiling beet pulp quality is not high.Beet pulp moisture content height can be solved by the method for the low raw material of water content such as oven dry or interpolation stalk, and the lactic acid bacteria number quantity not sufficient is the key issue that sugar beet pulp silages is badly in need of solution.
Summary of the invention
The lactic acid bacteria number quantity not sufficient causes the not high problem of ensiling beet pulp quality in the existing sugar beet pulp silages production in order to solve in the present invention, and a kind of compound bacteria additive for sugar beet pulp silages that provides and its production and application.
Compound bacteria additive for sugar beet pulp silages is formed by separating streptococcus acidi lactici and each 1 strain of lactobacillus of obtaining the fermented liquid of making from beet pulp, specifically be with streptococcus acidi lactici and lactobacillus respectively in the MRS liquid nutrient medium after the activation culture, activatory streptococcus acidi lactici and lactobacillus are pressed 40-60%: the weight percent of 40-60% mixes, and makes the sugar beet pulp silages microbe additive.Wherein, contain streptococcus acidi lactici 2.5 * 10 at least in every mlMRS liquid nutrient medium
7Cfu and lactobacillus 3.0 * 10
7Cfu.
Compound bacteria additive for sugar beet pulp silages prepares according to the following steps: step 1, beet pulp preparation of fermentation liquid: get fresh beet pulp, weight ratio in fresh beet pulp and distilled water is 2: 1 ratio thorough mixing, leave standstill 1-1.5h, after double gauze filters, filtered liquid adds by weight being beet pulp: mix behind the glucose of glucose=10: 1 ratio, fully stir and be placed on 30 ℃ of fermentation 48h, make fermented liquid; The separating of step 2, streptococcus acidi lactici and lactobacillus, purifying: doubly with beet pulp fermented liquid dilution 50-100, place the separate solid substratum, the medium pH value is 5.7-6.1, temperature is cultivated 48h down at 37-40 ℃, select that molten calcium circle is big, 2 of the bacterium colonies of substratum flavescence, be placed on MRS solid medium purifying respectively, through qualification test, obtain each 1 strain of streptococcus acidi lactici and lactobacillus, streptococcus acidi lactici behind the purifying and lactobacillus are preserved with the test tube that the MRS solid medium is housed; The activation of step 3, streptococcus acidi lactici and lactobacillus: streptococcus acidi lactici that purifying is good and lactobacillus are placed in the MRS liquid nutrient medium and activate, activation temperature is 35 ℃, soak time 24h contains streptococcus acidi lactici 2.5 * 107cfu and lactobacillus 3.0 * 107cfu in every mlMRS liquid nutrient medium when activation is finished at least; The weight percent of the preparation of step 4, sugar beet pulp silages microbe additive: activatory streptococcus acidi lactici and lactobacillus are pressed 40-60%: 40-60% mixes, and makes the sugar beet pulp silages microbe additive.Wherein, the separate solid culture medium prescription described in the step 2 is: extractum carnis 1g, and peptone 1g, yeast extract paste 1g, glucose 1g, tomato juice 20%, tween 80 0.05%, lime carbonate 2g, tetrabromo-mcresolsulfonphthalein 0.01%, agar 1.5g, pH are 5.7-6.1.MRS solid culture based formulas described in the step 2 is: extractum carnis 1g, peptone 1g, yeast extract paste 0.5g, glucose 2g, tween 80 0.1%, lime carbonate 2g, K
2HPO
40.2g, MgSO4.7H
2O 0.058g, MnSO
4.4H
2O 0.025g, sodium acetate 0.5g, dibasic ammonium citrate 0.2g, agar 1.5g, pH are 6.2,121 ℃ of sterilization 15min.MRS liquid culture based formulas described in the step 3 is: extractum carnis 1g, peptone 1g, yeast extract paste 0.5g, glucose 2g, tween 80 0.1%, lime carbonate 2g, K
2HPO40.2g, MgSO
4.7H
2O 0.058g, MnSO
4.4H
2O 0.025g, sodium acetate 0.5g, dibasic ammonium citrate 0.2g, pH are 6.2,121 ℃ of sterilization 15min.
The application of compound bacteria additive for sugar beet pulp silages: beet pulp makes its moisture content at 65-75% through after the drying treatment, and the amount that adds 10-15ml according to every kg beet pulp adds compound bacteria additive for sugar beet pulp silages, the application of fermenting through 40-50 days at normal temperatures.
Characteristics of the present invention are: (1) streptococcus acidi lactici and lactobacillus have very strong specific aim by separating in the beet pulp to sugar beet pulp silages; (2) streptococcus acidi lactici and lactobacillus are mixed in proportion, the two is all the dominant microflora in the sugar beet pulp silages process, and synergy obviously.
Beneficial effect of the present invention: comparative test result shows that the beet pulp of handling with compound bacteria additive for sugar beet pulp silages of the present invention fermented through 45 days, can significantly improve the sugar beet pulp silages effect:
(1) descends rapidly between test group pH value yeast phase, significantly be lower than the blank group.Test group pH value and blank group pH value are respectively 3.94 and 4.58, P＜0.05;
(2) test group significantly reduces than blank group mould and yeast quantity, is respectively 3.52 * 10
7Cfu/g and 5.87 * 10
7Cfu/g, P＜0.05;
(3) the test group ammonia-state nitrogen accounts for the ratio of total nitrogen, and promptly to compare difference extremely remarkable for VBN/TN and blank group, is respectively 10.48% and 14.67%, P＜0.01;
(4) test group lactic acid accounts for the ratio of total acid than blank group raising 9.24%, P＜0.05; Butyric acid content is than the remarkable reduction by 8.31% of blank group, P＜0.05.
Embodiment
Embodiment one: the present embodiment compound bacteria additive for sugar beet pulp silages is formed by separating streptococcus acidi lactici and each 1 strain of lactobacillus of obtaining the fermented liquid of making from beet pulp, specifically be that the two is respectively after the MRS liquid nutrient medium is cultivated, mix by the streptococcus acidi lactici of 40-60% and the lactobacillus of 40-60% according to weight percent, wherein, contain streptococcus acidi lactici 2.5 * 10 at least in every mlMRS liquid nutrient medium
7Cfu and lactobacillus 3.0 * 10
7Cfu.
Embodiment two: what present embodiment and embodiment one were different is: mixed by 40% streptococcus acidi lactici and 60% lactobacillus according to weight percent.Other parameter is identical with embodiment one.
Embodiment three: what present embodiment and embodiment one were different is: mixed by 50% streptococcus acidi lactici and 50% lactobacillus according to weight percent.Other parameter is identical with embodiment one.
Embodiment four: what present embodiment and embodiment one were different is: mixed by 60% streptococcus acidi lactici and 40% lactobacillus according to weight percent.Other parameter is identical with embodiment one.
Embodiment five: compound bacteria additive for sugar beet pulp silages prepares by following steps: step 1, beet pulp preparation of fermentation liquid: get fresh beet pulp, weight ratio in fresh beet pulp and distilled water is 2: 1 ratio thorough mixing, leave standstill 1-1.5h, after double gauze filters, filtered liquid adds by weight being beet pulp: mix behind the glucose of glucose=10: 1 ratio, fully stir and be placed on 30 ℃ of fermentation 48h, make fermented liquid; The separating of step 2, streptococcus acidi lactici and lactobacillus, purifying: doubly with beet pulp fermented liquid dilution 50-100, place the separate solid substratum, the medium pH value is 5.7-6.1, temperature is cultivated 48h down at 37-40 ℃, select that molten calcium circle is big, 2 of the bacterium colonies of substratum flavescence, be placed on MRS solid medium purifying respectively, through qualification test, obtain each 1 strain of streptococcus acidi lactici and lactobacillus, streptococcus acidi lactici behind the purifying and lactobacillus are preserved with the test tube that the MRS solid medium is housed; The activation of step 3, streptococcus acidi lactici and lactobacillus: streptococcus acidi lactici that purifying is good and lactobacillus are placed in the MRS liquid nutrient medium and activate, activation temperature is 35 ℃, soak time 24h contains streptococcus acidi lactici 2.5 * 10 in every mlMRS liquid nutrient medium when activation is finished at least
7Cfu and lactobacillus 3.0 * 10
7Cfu; The weight percent of the preparation of step 4, sugar beet pulp silages microbe additive: activatory streptococcus acidi lactici and lactobacillus are pressed 40-60%: 40-60% mixes, and makes the sugar beet pulp silages microbe additive.Wherein, the separate solid culture medium prescription described in the step 2 is: extractum carnis 1g, and peptone 1g, yeast extract paste 1g, glucose 1g, tomato juice 20%, tween 80 0.05%, lime carbonate 2g, tetrabromo-mcresolsulfonphthalein 0.01%, agar 1.5g, pH are 5.7-6.1.The described MRS solid culture of step 2 based formulas is: extractum carnis 1g, peptone 1g, yeast extract paste 0.5g, glucose 2g, tween 80 0.1%, lime carbonate 2g, K
2HPO
40.2g, MgSO
4.7H
2O 0.058g, MnSO
4.4H
2O 0.025g, sodium acetate 0.5g, dibasic ammonium citrate 0.2g, agar 1.5g, pH are 6.2,121 ℃ of sterilization 15min.MRS liquid culture based formulas described in the step 3 is: extractum carnis 1g, peptone 1g, yeast extract paste 0.5g, glucose 2g, tween 80 0.1%, lime carbonate 2g, K
2HPO
40.2g, MgSO4.7H2O0.058g, MnSO
4.4H
2O 0.025g, sodium acetate 0.5g, dibasic ammonium citrate 0.2g, pH are 6.2,121 ℃ of sterilization 15min.
Embodiment six: beet pulp makes its moisture content at 65-75% through after the drying treatment, and the amount that adds 10-15ml according to every kg beet pulp adds compound bacteria additive for sugar beet pulp silages, the application of fermenting through 40-50 days at normal temperatures.
Embodiment seven: beet pulp makes its moisture content 70% through after the drying treatment, and the amount that adds 12ml according to every kg beet pulp adds compound bacteria additive for sugar beet pulp silages, the application of fermenting through 45 days at normal temperatures.
Claims (7)
1, a kind of compound bacteria additive for sugar beet pulp silages is characterized in that compound bacteria additive for sugar beet pulp silages is mixed by the streptococcus acidi lactici of 40-60% and the lactobacillus of 40-60% according to weight percent.
2, a kind of compound bacteria additive for sugar beet pulp silages according to claim 1 is characterized in that compound bacteria additive for sugar beet pulp silages is mixed by 40% streptococcus acidi lactici and 60% lactobacillus by weight percentage.
3, a kind of compound bacteria additive for sugar beet pulp silages according to claim 1 is characterized in that compound bacteria additive for sugar beet pulp silages is mixed by 50% streptococcus acidi lactici and 50% lactobacillus by weight percentage.
4, a kind of compound bacteria additive for sugar beet pulp silages according to claim 1 is characterized in that compound bacteria additive for sugar beet pulp silages is mixed by 60% streptococcus acidi lactici and 40% lactobacillus by weight percentage.
5, a kind of preparation method of compound bacteria additive for sugar beet pulp silages, it is characterized in that compound bacteria additive for sugar beet pulp silages prepares according to the following steps: step 1, beet pulp preparation of fermentation liquid: get fresh beet pulp, weight ratio in fresh beet pulp and distilled water is 2: 1 ratio thorough mixing, leave standstill 1-1.5h, after double gauze filters, filtered liquid adds by weight being beet pulp: mix behind the glucose of glucose=10: 1 ratio, fully stir and be placed on 30 ℃ of fermentation 48h, make fermented liquid; The separating of step 2, streptococcus acidi lactici and lactobacillus, purifying: doubly with beet pulp fermented liquid dilution 50-100, place the separate solid substratum, the medium pH value is 5.7-6.1, temperature is cultivated 48h down at 37-40 ℃, select that molten calcium circle is big, 2 of the bacterium colonies of substratum flavescence, be placed on MRS solid medium purifying respectively, through qualification test, obtain each 1 strain of streptococcus acidi lactici and lactobacillus, streptococcus acidi lactici behind the purifying and lactobacillus are preserved with the test tube that the MRS solid medium is housed; The activation of step 3, streptococcus acidi lactici and lactobacillus: streptococcus acidi lactici that purifying is good and lactobacillus are placed in the MRS liquid nutrient medium and activate, activation temperature is 35 ℃, soak time 24h contains streptococcus acidi lactici 2.5 * 10 in every mlMRS liquid nutrient medium when activation is finished at least
7Cfu and lactobacillus 3.0 * 10
7Cfu; The weight percent of the preparation of step 4, sugar beet pulp silages microbe additive: activatory streptococcus acidi lactici and lactobacillus are pressed 40-60%: 40-60% mixes, and makes the sugar beet pulp silages microbe additive.
6, a kind of application of compound bacteria additive for sugar beet pulp silages, it is characterized in that beet pulp makes its moisture content at 65-75% through after the drying treatment, the amount that adds 10-15ml according to every kg beet pulp adds compound bacteria additive for sugar beet pulp silages, the application of fermenting through 40-50 days at normal temperatures.
7, the application of a kind of compound bacteria additive for sugar beet pulp silages according to claim 6, it is characterized in that beet pulp makes its moisture content 70% through after the drying treatment, the amount that adds 12ml according to every kg beet pulp adds compound bacteria additive for sugar beet pulp silages, the application of fermenting through 45 days at normal temperatures.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910072720A CN101629150A (en) | 2009-08-21 | 2009-08-21 | Compound bacteria additive for sugar beet pulp silages and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910072720A CN101629150A (en) | 2009-08-21 | 2009-08-21 | Compound bacteria additive for sugar beet pulp silages and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101629150A true CN101629150A (en) | 2010-01-20 |
Family
ID=41574430
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910072720A Pending CN101629150A (en) | 2009-08-21 | 2009-08-21 | Compound bacteria additive for sugar beet pulp silages and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101629150A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102450525A (en) * | 2010-10-25 | 2012-05-16 | 董志国 | Leguminous forage silage complex bacteria additive |
| CN103652497A (en) * | 2013-12-18 | 2014-03-26 | 青岛百瑞吉生物工程有限公司 | Feed additive for aquatic organisms and preparation method of feed additive |
| RU2511994C2 (en) * | 2012-06-14 | 2014-04-10 | Общество с ограниченной ответственностью "БИОТРОФ" | Method of conservation of beet pulp |
| CN103859154A (en) * | 2014-02-26 | 2014-06-18 | 铜仁职业技术学院 | Production method of silage |
| CN105238698A (en) * | 2015-08-17 | 2016-01-13 | 南京师范大学 | Method for transferring beet powder into enzyme yeast and health care products by using eurotium |
| CN106418498A (en) * | 2016-08-31 | 2017-02-22 | 唐妙红 | Method for preparing enzyme by using lemons and galangal as main materials |
-
2009
- 2009-08-21 CN CN200910072720A patent/CN101629150A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102450525A (en) * | 2010-10-25 | 2012-05-16 | 董志国 | Leguminous forage silage complex bacteria additive |
| RU2511994C2 (en) * | 2012-06-14 | 2014-04-10 | Общество с ограниченной ответственностью "БИОТРОФ" | Method of conservation of beet pulp |
| CN103652497A (en) * | 2013-12-18 | 2014-03-26 | 青岛百瑞吉生物工程有限公司 | Feed additive for aquatic organisms and preparation method of feed additive |
| CN103652497B (en) * | 2013-12-18 | 2015-08-05 | 青岛百瑞吉生物工程有限公司 | A kind of aquatic biological feed additive and preparation method thereof |
| CN103859154A (en) * | 2014-02-26 | 2014-06-18 | 铜仁职业技术学院 | Production method of silage |
| CN105238698A (en) * | 2015-08-17 | 2016-01-13 | 南京师范大学 | Method for transferring beet powder into enzyme yeast and health care products by using eurotium |
| CN106418498A (en) * | 2016-08-31 | 2017-02-22 | 唐妙红 | Method for preparing enzyme by using lemons and galangal as main materials |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108041289B (en) | Silage additive special for paper mulberry and application thereof | |
| CN101629150A (en) | Compound bacteria additive for sugar beet pulp silages and preparation method and application thereof | |
| CN103238672A (en) | Dilute cream cheese and preparation method thereof | |
| CN1224333C (en) | Special-purpose additive for ensiling corn | |
| CN102907572A (en) | Banana silage microbial additive and preparation method thereof | |
| CN106190693A (en) | A kind of fruit wine preparation method rich in anthocyanidin fruit | |
| CN1324129C (en) | Multiple strain microorganism ferment production method and its uses in cow fine fodder | |
| CN102250807A (en) | Microbial agent for Chinese silvergrass ensilage as well as preparation method and application thereof | |
| CN109770060A (en) | A method of preparing soybean stalk feed | |
| CN113693144A (en) | Kangpu tea mother liquor and preparation method thereof | |
| CN111362736B (en) | Preparation method of grape waste fruit enzyme for fertilizer, prepared grape waste fruit enzyme and application of grape waste fruit enzyme | |
| CN113796455A (en) | Process for producing feed by using crop straws | |
| CN113261609A (en) | Production method of fermented feed | |
| CN105961850A (en) | A fermentation accelerant for feed prepared by utilizing air-dried bagasse | |
| CN111642640A (en) | Broussonetia papyrifera feed vegetable protein starter culture for pigs and preparation method thereof | |
| CN112266883A (en) | Zymophyte liquid for whole-plant fermentation of hybrid paper mulberry, and preparation method and application thereof | |
| CN105695287B (en) | A kind of selenium-rich agaric mulberry health care black vinegar | |
| CN105581218A (en) | Wild blueberry juice flavor and quality improving method by using lactobacillus plantarum fermentation | |
| CN113519705A (en) | Silage and preparation method thereof | |
| CN110973353B (en) | Solid-state fermentation alfalfa meal feed and preparation method and application thereof | |
| CN114946997A (en) | Method for producing compound feed by fermenting mixed strains | |
| CN107549557A (en) | A kind of processing method of flavor blueberry juice | |
| CN112772770A (en) | Preparation method of stevia rebaudiana fermentation product and milch goat feed | |
| CN1320106C (en) | Preparation method of composite bacterial additive of alfalfa ensilage | |
| CN111202168B (en) | Bioactive feed fermented by soybean yellow serofluid and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20100120 |