CN102037894A - Method for breeding and screening astragalus sinicus varieties with high selenium content - Google Patents

Method for breeding and screening astragalus sinicus varieties with high selenium content Download PDF

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Publication number
CN102037894A
CN102037894A CN2010102936836A CN201010293683A CN102037894A CN 102037894 A CN102037894 A CN 102037894A CN 2010102936836 A CN2010102936836 A CN 2010102936836A CN 201010293683 A CN201010293683 A CN 201010293683A CN 102037894 A CN102037894 A CN 102037894A
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China
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selenium
varieties
qrs
milk vetch
chinese milk
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CN2010102936836A
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汪德尚
姚自鸣
尹德柱
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汪德尚
姚自鸣
尹德柱
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Abstract

The invention relates to a method for breeding and screening astragalus sinicus varieties with high selenium content, which comprises the following steps of: screening the selenium QRS values of the conventional high-quality astragalus sinicus varieties in China by using a quantum resonance spectrometer (QRS), hybridizing varieties of which the selenium QRS values reach +3, and further screening the selenium QRS values of first filial generation; planting the first filial generation of which the selenium QRS values reach +4, spraying selenium-rich nutrient solution with different concentrations on leaf surfaces twice in the growth period, and screening the selenium QRS values of seeds after the seeds are mature, wherein varieties of which the selenium QRS values of plants and the seeds are in the range of +5 to +7 are regarded as the astragalus sinicus varieties with high selenium content, and varieties of which the selenium QRS values are less than +5 are regarded as the common selenium-rich astragalus sinicus varieties; and performing corresponding biochemical analytical determination to determine the selenium content of each variety with high selenium content. The method has the advantages that: natural astragalus sinicus varieties with high selenium enrichment capacity are planted to convert exogenous inorganic selenium into organic selenium with high bioavailability, and are taken as selenium carriers to prepare green manure, feed additives and food additives, and selenium-rich agricultural products are produced through secondary conversion, so that the problem that food is lack of selenium is solved.

Description

A kind of selenium-enriched Chinese milk vetch breed breeding screening technique
Technical field:
The present invention relates to relax enrich the selenium Chinese milk vetch No. 1, No. 6, Shu Fengfu selenium Chinese milk vetch, No. 8 kind technology of Shu Fengfu selenium Chinese milk vetch of a kind of Chinese milk vetch by Chinese milk vetch kind system finishing, intervarietal cross and selenium-enriched testcross and screening being gone out selenium-enriched and high biological transformation degree.
Background technology:
The World Health Organization (WHO) in 1973 announces that selenium is trace element essential in the animal life, it is that food enrichment has been issued standard GB 14880-1994 that China in 1994 allows to add selenium in food, FDA (Food and Drug Adminstration) (FDA) approval selenium in 2003 allows the carcinous matter that presses down of selemium nutrition product sign selenium for pressing down the cancer agent.People recognize that more and more the selenium element is to keeping the important function that the human life activity plays in recent years, selenium is the important component part of glutathione peroxidase, be again antioxidant simultaneously, can eliminate too much free radical and MDA in the body, mend selenium and can increase glutathione peroxidase (GSHPX), the activity of superoxide dismutase (SOD), thus reach the purpose that improves body's immunity, resistance against diseases, delays senility.Threaten four big diseases of human life's health: hepatopathy, cancer, cardiovascular and cerebrovascular diseases, disease of digestive tract are all relevant with scarce selenium.Therefore development and exploitation se-rich health food products and natural selenium-rich food have become a kind of trend.
The benefit selenium product and the production method thereof of selling on the domestic and international market mainly contains at present: be inorganic selenium tablet, capsule or the oral liquid of raw material processing with the mineral salt natrium nitrosum directly 1), this type of selenium product absorption of human body is poor, and bioavilability is low; 2) selenium yeast that adopts crops fermenting raw materials technology to produce is tablet, capsule or the suspended emulsion of raw material processing, and this series products needs to add chemical compositions such as auxiliary agent, auxiliary material, stabilizing agent, preservative, flavouring in process of production.Cost an arm and a leg and should not eat for a long time; 3) by plant, cereal on its blade face or seedling stage, heading stage spray selenium nutrient solution and utilize the biological transformation of plant to obtain.But this inorganic selenium technology that directly sprays exists most of crops low to selenium biological transformation degree, and inorganic selenium toxicity is too big, is difficult to grasp an amount of application difficult problem, is difficult for continuous administration, otherwise very easily causes the inorganic selenium environmental pollution harmful to people and animals, is not suitable for generally promoting.The selenium-enriched tea leaf of Tui Chuing, rice, wheat, corn, paddy rice, vegetables etc. from the rich selenium soil place of production (as Shitai County, Anhui, Ziyang, Shaanxi, enshi), all belong to this except on a small quantity in the market.4) selenium-enriched pork of the pig of culturing by Se-enriched feedstuff, ox, sheep, bird production, beef, mutton, milk, poultry meat egg etc., contain and easily be the animal selenoprotein of absorption of human body, it is best in the world at present benefit selenium functional food, but can not directly absorb inorganic selenium because animal and human's body is the same, can not use greatly animal body toxicity.Have only and change into organic selenium albumen by forage plant and could absorb be transformed by fowl poultry safety, but most of forage plants are all low to the organic transformation degree of selenium, be only green manure be again that the Chinese milk vetch of feed has stronger rich cumulative power to selenium, and organic transformation degree height is desirable livestock and poultry Se-enriched feedstuff and feed addictive.
2001~2003 years Anhui Province academy of agricultural sciences's clay fertilizer institute carries out rich selenium high yield research to more domestic main good Chinese milk vetch kinds, use an amount of sodium selenite solution aborning early stage, the bright careless output of the rich selenium Chinese milk vetch of continuous 3 years Spraying Se nutrient solutions rises to average 4634.4kg/ mu, do not increased by 1.67 times and only illustrated that external source executes selenium and Chinese milk vetch is had the effect that significantly improves per unit area yield than there being external source execute the average 1737.7kg/ mu of selenium in 1995~1997 years 3 years, selenium rich ability size between kind not being carried out examination research, is not that purpose is carried out the breed breeding screening with high richness more.We were bearing on academy of agricultural sciences, the Anhui Province clay fertilizer institute Chinese milk vetch breeding basis in 2005, in the province academy of agricultural sciences, economize micro-expert Yao Ziming, Wang Guangyi professor's prompting and lead down, to the Chinese milk vetch kind of having grasped, carry out rich selenium Chinese milk vetch kind examination seed selection by product intermolecular hybrid and test cross, find and select the general strong 2 times selenium-enriched Chinese milk vetch kind of Chinese milk vetch kind selenium rich ability.Solved and utilized selenium-enriched Chinese milk vetch secondary to transform safety problem.
The present technique characteristics are to cultivate and filter out selenium-enriched purple cloud kind, strengthen spray selenium nutrient solution twice by plantation external source blade face, make Chinese milk vetch hay Se content reach the 4.403mg/kg organic selenium and account for 77%/become that selenium-enriched food is inexpensive, the plain additive of selemium nutrition of safety, nutrition.Still there is not identical technology report at present both at home and abroad.
Summary of the invention
The present invention will solve a kind of relaxing by the Chinese milk vetch that Chinese milk vetch kind system finishing, intervarietal cross and selenium-enriched testcross and screening is gone out selenium-enriched and high biological transformation degree and enrich the selenium Chinese milk vetch.
Main feature of the present invention is: (1), domestic existing good Chinese milk vetch kind is carried out quantizing resonance instrument examination selenium QRS value, reach+3 values in species hybriding, and further an assorted generation is carried out the examination of selenium QRS value; (2), to the QRS value reach+4 first-filial generation plants, selenium-rich nutritive liquid at twice variable concentrations of foliage-spray in vegetative period, reach 40-50cm and enter the Sheng phase harvesting of blooming, carry out the QRS pH-value determination pH, again seed is carried out the examination of selenium QRS value after (3) seed maturation, plant and seed selenium QRS value are considered as selenium-enriched Chinese milk vetch kind between+5-+7, less than+5 be common rich selenium Chinese milk vetch kind, and finally carry out corresponding biochemical analysis and measure, determine each selenium-enriched kind selenium content; (4) selenium-enriched Chinese milk vetch plant harvesting back solarization dry grinding, granulation become the feed additive rich in selenium, raise the rich selenium poultry and egg of Production of Livestock and Poultry; Selenium-enriched Chinese milk vetch stub land is because the biological solid every 667m of N 2Produce the N fertilizer that is equivalent to 20kg urea, for the second stubble crop utilization, spread all over its biomass of underground root system and acrial part simultaneously and almost be equal to, its Se content and acrial part are equal in theory.
Main feature of the present invention also is: at the selenium-rich nutritive liquid of twice variable concentrations of foliage-spray in vegetative period, be period: shooting stage and flowering stage.
Main feature of the present invention also is: the selenium-rich nutritive liquid that sprays two variable concentrations vegetative period for twice is: 5g/100ml selenium nutrient solution, every 667m 2Spray 200ml and 400ml solution.
Main feature of the present invention also is: selenium-rich nutritive liquid composition comprises: inorganic selenium salt, organic zinc, surfactant.
Beneficial effect of the present invention: the Chinese milk vetch kind by natural high enrichment selenium ability is high bioavilability organic selenium through plantation conversion external source inorganic selenium, make green manure, make feed addictive, cook food additives secondary conversion production Se-rich farm products with this carrier, solve the poor scarce problem of food selenium as selenium.
Embodiment
In embodiment the present invention is described in further detail below
Chinese milk vetch variety collection → varieties of plant purification and rejuvenation → kind selenium rich ability examination → kind selenium QRS value reaches+3 above persons carry out extensively hybridization between kind → to F1 generation carry out the examination of selenium QRS value → to F1 for selenium QRS value reach+4 seed plantation → external source blade face sprays two variable concentrations (5g/100ml selenium nutrient solution, every 667m for twice 2Spray 200ml and 400ml solution) selenium nutrient solution → Chinese milk vetch enter full-bloom stage harvesting and dry and measure plant selenium QRS value, ripe results seed mensuration seed selenium QRS value reaches+5-+7 is selenium-enriched Chinese milk vetch product.
→ processing is pulverized granulation and is packaged into feed additive rich in selenium finished product or food additive rich in selenium finished product
→ make fertilizer can imbed plow layer by complete stool
→ do seed soaking agent, the broken making beating fermentation of the bright grass meal of full-bloom stage back has shell to soak 48-60 hour, nothing to soak shell 12-18 hour by mixing with natural pond liquid at 1: 1 to soak seed.
No. 1 selenium-enriched Chinese milk vetch kind of Shu Feng (QRS value+6 +Biochemical measurement contains selenium 4.403mg/kg) sowing, jointing to every mu of initial bloom stage uses selenium-rich nutritive liquid 200~400ml to be made into the aqueous solution and sprays 2 times, enters the full-bloom stage harvesting or the back results dry straw of collecting seed, and (moisture 8%) powder pure granulation packing in fully dry back becomes the animal and fowl fodder additive rich in selenium.Total Se content 200~500ug/kg, every physical and chemical index of product of the present invention is as follows after testing:
The selenium-enriched Chinese milk vetch variety production of table one Se-enriched feedstuff additive physical and chemical index
The selenium-enriched Chinese milk vetch of table two is produced Se-enriched feedstuff additive microbiological indicator

Claims (4)

1. selenium-enriched Chinese milk vetch breed breeding screening technique, it is characterized in that: (1), domestic existing good Chinese milk vetch kind is carried out quantizing resonance instrument examination selenium QRS value, reach+3 values in species hybriding, and further an assorted generation is carried out the examination of selenium QRS value; (2), to the QRS value reach+4 first-filial generation plants, selenium-rich nutritive liquid at twice variable concentrations of foliage-spray in vegetative period, reach 40-50cm and enter the Sheng phase harvesting of blooming, carry out the QRS pH-value determination pH, again seed is carried out the examination of selenium QRS value after (3) seed maturation, plant and seed selenium QRS value are considered as selenium-enriched Chinese milk vetch kind between+5-+7, less than+5 be common rich selenium Chinese milk vetch kind, and finally carry out corresponding biochemical analysis and measure, determine each selenium-enriched kind selenium content; (4) selenium-enriched Chinese milk vetch plant harvesting back solarization dry grinding, granulation become the feed additive rich in selenium, raise the rich selenium poultry and egg of Production of Livestock and Poultry; Selenium-enriched Chinese milk vetch stub land is because the biological solid every 667m of N 2Produce the N fertilizer of suitable hand 20kg urea, for the second stubble crop utilization, spread all over its biomass of underground root system and acrial part simultaneously and almost be equal to, its Se content and acrial part are equal in theory.
2. a kind of selenium-enriched Chinese milk vetch breed breeding screening technique according to claim 1, its feature also is: at the selenium-rich nutritive liquid of twice variable concentrations of foliage-spray in vegetative period, be period: shooting stage and flowering stage.
3. according to claim or 2 described a kind of selenium-enriched Chinese milk vetch breed breeding screening techniques, its feature also is: the selenium-rich nutritive liquid that sprays two variable concentrations vegetative period for twice is: 5g/100ml selenium nutrient solution, every 667m 2Spray 200ml and 400ml solution.
4. a kind of selenium-enriched Chinese milk vetch breed breeding screening technique according to claim 1, its feature also are: selenium-rich nutritive liquid composition comprises: inorganic selenium salt, organic zinc, surfactant.
CN2010102936836A 2010-09-21 2010-09-21 Method for breeding and screening astragalus sinicus varieties with high selenium content Pending CN102037894A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102423046A (en) * 2011-12-19 2012-04-25 苏州硒谷科技有限公司 Selenium-enriched milk vetch plant material, preparation method and application thereof
CN104396734A (en) * 2014-11-18 2015-03-11 湖南省土壤肥料研究所 Breeding method of milk vetch
CN104871844A (en) * 2015-06-03 2015-09-02 长江大学 Screening method of rice variety resource selenium enrichment performance
CN106993530A (en) * 2017-05-24 2017-08-01 福建农林大学 A kind of method of selenium-rich Job's tears breed breeding
CN108409472A (en) * 2018-06-11 2018-08-17 凤阳县兴科农业生态发展有限公司 A kind of herbal medicine plantation organic iron fertilizer

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
《量子共振检测(QRS)应用研究》 20071231 王广仪 《节能环保 和谐发展--2007年中国科技年会论文集(二)》 1-6 1-4 , *
余林生等: "富硒紫云英的栽培及其在养蜂业上的应用", 《蜜蜂杂志》 *
李兆勇等: "紫云英的栽培与利用", 《农业装备技术》 *
王广仪: "《节能环保 和谐发展——2007年中国科技年会论文集(二)》", 《量子共振检测(QRS)应用研究》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102423046A (en) * 2011-12-19 2012-04-25 苏州硒谷科技有限公司 Selenium-enriched milk vetch plant material, preparation method and application thereof
CN104396734A (en) * 2014-11-18 2015-03-11 湖南省土壤肥料研究所 Breeding method of milk vetch
CN104871844A (en) * 2015-06-03 2015-09-02 长江大学 Screening method of rice variety resource selenium enrichment performance
CN106993530A (en) * 2017-05-24 2017-08-01 福建农林大学 A kind of method of selenium-rich Job's tears breed breeding
CN108409472A (en) * 2018-06-11 2018-08-17 凤阳县兴科农业生态发展有限公司 A kind of herbal medicine plantation organic iron fertilizer

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Application publication date: 20110504